Cucsat.G7501 (gene) Cucumber (B10) v3

Overview
NameCucsat.G7501
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmitogen-activated protein kinase kinase 2
Locationctg1528: 3537361 .. 3541875 (+)
RNA-Seq ExpressionCucsat.G7501
SyntenyCucsat.G7501
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATGAATGTAGGTGAGGCATATTTAAATTGGATTTTGCAATATTGGGAGTTCGAAAAGTTAGAATTTTGTAAGAAAAATCAAAGGAAAAAGAAAAGTCAAAGATTCAAAAGAAGAAAAGTAAACGGTCCCGCATCCTCCAATCCAATCCAACCAAGAACAGCCCTATTTTTCTTTTTCTTTTCAATGTTTAAATCAGCTCTTAGTGTCACAAAAATGGTGTAATTAGGGTTTTTGAGTTTGGATTTTTTGTAAATTCTATCAGTTTCATTCTCAACTACTGCTCAACACTTGATTAATTCATATATATAAACAAATTCTTGACATTTCTCTGCAATTATCTCTCTCTTTCTCTCTCTACATCGCAAAAGGCTTCAATCCGAGTATCAATTCTACACGGTGGTAGTCTGGAAGAGATCAGCCATCAATGAGGAAAGGAGGCTTCAGCAACAACCTCAATCTCAAGCTCAATCTTCCTAAAGAAGACCAATCCATAGCGACGTTCCTGTAATCTTTCTCTCTGTGTGCTTGTGCTTGTGTGTGTATGTGTGTAAGTGAAAATTTCTGATGAATCTGGAATCTGGGTTTGTTTTTCTTGTGTAGAACGCAGAGTGGCACGTTTAAAGATGGTGATCTGCTTGTAAACAGAGATGGGGTTCGGATTGTTTCTCAAAGCGAAGTTGAAGCAGTAAGAGTTCTTAGTTTTGTAGCTTAGTGTGACTTTAGAGGACTATAATGTGCTGTAGTTTTCAATGTTATGGCTGAGGGAATTTGGATTTTAGCTCATTAACTTTTCAAGTATGGGTTTTCTTGCTACGTATTAAGTATTTGTTTGGTTTCTGTTGAGTTGCTTAAAGTATTCTGCAGTGAAGTATGGAATTACAAATCCGTATACTATGCTTGATGTACTGTGCTCACTTCACTATGGGTTTCATTTGTGATTGGGTTTCATTTGTTACTAACTCCTAGTTAACTAAATCTCTGTGTGATATCGTTTTCATTTTTAGGTTCTATTTCTTAAAATTATGCTTGTTGCTTTATCATTTCTTTACTATGGTTTTCATTTCTCTTTAAGAATCATTTAGATTCAAAGCCAAATTCCAAATAAACAAAATCACAATCTACATTTTTTTTATTTAGTTTTCAAAAACTTGGGTTTGATAACTTTTCAAGGGAGTAGATGACAAAATAAAGGAACTCATAAGTGGAAATAGTGTTTGTAATCTTTATTTTTTAAATTTAAGAATTAGGTGGTTATCATTGAAATTCAGACTAATTGATTGAGGTATATAATACGTGAGATTCCTATACTGAACTTGTTTGTTTTTCAACACATAGGGAACAAAGTGAAAAAGTGGTTGATTATGTTGTCTTGTGAACTATCTTGTGTATATTAAGCTACTAACTTGTTTTCCTTCTACCTATGTGTTCAGCCACCTCCGATTAAGCCTACAGATGATCAGTTGAGTTTAGCGGACATAGACATTATTAAAGTCATTGGAAAAGGAAATGGTGGTACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTACTGCACAACTTAAACATTATTGAAATTCTCTGTCTCTGACTTCTATGATTTATATACTTTATAGTTGAGGCTTCAAAAAGTGGTTAGAGCATCTTAATCATCATTATATTGAAGGTATATTTTTCTTGTGAACTGAAAGATGGTTAATAATAAATAAAAAGAAAAGAAGGTATATTTTTCTTTCACCAGCAGTTTCACATTCCTATTCGTACACTACCCATTAGAAATCTTAGAATCAAGAAAAAAAGTAGTAACTATCTGCATTAATTAGTAAAAAAAATATTTCAAACCATATCAAATACCAAATCATTTCGTCTACTCTTTCAGGTAATTCAGATGAAAATTGAAGAGTCTCACCGCAAGCAGATTGCACAAGAACTGAAAATCAATCAATCAGCGCAGTGCCCTTATGTTGTTGTCTGTTACCAGTCTTTCTATGATAATGGATCAATATATATCATCCTAGAGTACATGGATGGAGGATCTTTAGCAGATTTTTTAAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCCTTTGTAAGCAGGTTGGTACAATAAGTCTTTGCTTGGATTTGGAGAATTCTTTTTTAATTAATTTATTTTTCAATTTTAGGAGATAATATAAGATTTCCTTTTTAAACCCCTTTCTCCTTCCATACAGGTACTGAAGGGGTTGTCTTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCAAATTTATTAATAAACCATAGAGGGGAAGTCAAGATTACTGACTTTGGTGTGAGTGCAATTATGGAAAACACATATGAAGAGGCTAATACTTTTGTTGGCACGTATAACTATATGTCCGTGAGTATCAATTCTCAATATATTCGGATCAATACAATTCTCGGGCTGTCTTTCCTTTATTTTTTCTGGTAAAGCTGTACGTTTTTCCTTCCATACCTATGTGTATAACTGACTGGTGTCTCTATGTATTGTCGTTTATAGCCAGAGAGAATTGTGGGAGAGGGATATGACAATAAAAGTGACATTTGGAGCTTGGGTCTGATATTACTTGAGTGTGCAACCGGAAAGTTTCCTTATTCTCCACCCGGGCAAGATGGAGGATGGGTTAATTTTTATGAGCTTATGGAAGCCATTGTTGAAGGCGAACCTCCTTCTGCTCCGGCTGACCAATTTACTCCTGAATTCTGTTCATTTATTTCTGCATGGTAAGGACCCAACTTTTCCTATAGTCCCTTTTCCTGAGATTATGACAATCATTATATGTACTTCATGTAGAAATTTCTAAATGTAACTGTAAAGTAGGTATTTTCTTATACTCTAAGCTTATTTCAAGTGTTTTTACATTATGAGATTTCTGACTCAGAAACCTGTTATCTTTATGAATATCAGTGTGCAAACAGACCCAAAGAATAGACTGTCAGCACGTGAACTTTTGGTATATAATTCTTCCTTTCTACATATTGATAAGCAAGATGTCAACACAATAGAAAAAATAAATCTGAATTTTGTCTTCACTTTTTGTGTGACTTTCAGGAACATCCTTTCATCAAGATGTATGAAGATAAGGATATTGATCTATCATCTTACTTCAATGATGCAGGATCTCCACTTGCAACTTTCTAAATTTTATCTGGTAAGTTAGGAACTACATCCCTTTTAAGTTTACCTTTCAAAAAAATAAGAAAGAAAGAAAGAAAAAACTACGTCTCTTTTAAAGCTTCCTCTTGATATCTTTTTTAATTCTGTTTTGAATTACGTGATTGAGGTGATCATTTCTTGCAACCGAAGCTATCATTCAGTGTAATGAAGTGGAAATTTCTGTCTTCATTATTTGCATGCTAAACATATATTTTAAACTATGTTTTCCCCTTATAGCTCGTTTATAAATTTTTATTTTATTTTTAACCAACTGTTTATCTCCTTTGGTACTTTGAGATTCTTCTGCTTTCAGCTTTTTGCCAGTTCCCATCATAGCTTTGTATGACTGTGTGCATTTTTTATATGATTAAATTAAGTACGCCAGCTTTCTTTGGGACAATAGACTTAAAGTTAGCTGCATTTGACTGTGTATAGCAAGATGAAGATCAAACTTCTGACCTTTTAGGCCATATAAGAGCACACAAGGACAAGCGGCTTAGATGAAACCTGTTTCAAACTTATATGGTAGGTTAATTTAAAAAATCAGATCTACAGAAAGTCGCTAGAAATGGAATCTATGTATATAGTATGGTGATATTTCTGACTATCACTTTACTTGAATGATGAAATATAGAAATGTTAGGTAAAAATATTATGCTATGGTTAATGGCTCCAACATTCATGGCCAATTGAAATACCTAGAACTAATGAATGTTGGAATTTTTTGATATTGTTTCATGTCCTTCTGTCTCTGTTTCTCTCATTCAAGCTTTTACCCATTAACTGGTGTTTATGCTGATAATGCATCATTTTCTCGACAGTTGATCGTCTCTGGCTCGTACACTGGAATCTTATTTGTTTGCTGCTTGCTGCAAGGATGGACGAGGAGTCCGCTGGGATCTCGGTTTCTCATGAATGCTGCTTGTTTTACTTCAGTTTGTGTCTTAATGAAAGAGGTTTCAATCAAAACTGGATGATATAGTTGTTCCTATGCTGGTCACTAATTTGGATGATTCTGATCCTTCTTGTGGATGGCTCAGAATGCTAAACGGGATATGATGTTGTGTAAAGAGATTTCAAATCATGGCCATTTCTTGTAACATTTTGTAATCCAATTCCACCGCTACTCATGTAAAATTTGAAGCATCAAAGGAAAATAAATACATATTGTTATCTCAAGTTTCTGTCTTGTATTGTTATCTCAAGTTAAATTTGCTCGTGTAGCTTCTAAACATTATTAGGATGGCCATAGCCATCCACAAGAGAAAGAACACAAGTACGCAGTATAAGAGAATT

Coding sequence (CDS)

ATGAGGAAAGGAGGCTTCAGCAACAACCTCAATCTCAAGCTCAATCTTCCTAAAGAAGACCAATCCATAGCGACGTTCCTAACGCAGAGTGGCACGTTTAAAGATGGTGATCTGCTTGTAAACAGAGATGGGGTTCGGATTGTTTCTCAAAGCGAAGTTGAAGCACCACCTCCGATTAAGCCTACAGATGATCAGTTGAGTTTAGCGGACATAGACATTATTAAAGTCATTGGAAAAGGAAATGGTGGTACTGTGCAATTAGTTCAGCACAAATGGACTGCTCAGTTTTTTGCATTGAAGGTAATTCAGATGAAAATTGAAGAGTCTCACCGCAAGCAGATTGCACAAGAACTGAAAATCAATCAATCAGCGCAGTGCCCTTATGTTGTTGTCTGTTACCAGTCTTTCTATGATAATGGATCAATATATATCATCCTAGAGTACATGGATGGAGGATCTTTAGCAGATTTTTTAAAAAAGGTTAAAAAAATTGAAGAACCATATCTTGCTGCCCTTTGTAAGCAGGTACTGAAGGGGTTGTCTTACCTTCACCATGAAAGACACATCATCCATAGGGACTTGAAGCCTTCAAATTTATTAATAAACCATAGAGGGGAAGTCAAGATTACTGACTTTGGTGTGAGTGCAATTATGGAAAACACATATGAAGAGGCTAATACTTTTGTTGGCACGTATAACTATATGTCCCCAGAGAGAATTGTGGGAGAGGGATATGACAATAAAAGTGACATTTGGAGCTTGGGTCTGATATTACTTGAGTGTGCAACCGGAAAGTTTCCTTATTCTCCACCCGGGCAAGATGGAGGATGGGTTAATTTTTATGAGCTTATGGAAGCCATTGTTGAAGGCGAACCTCCTTCTGCTCCGGCTGACCAATTTACTCCTGAATTCTGTTCATTTATTTCTGCATGTGTGCAAACAGACCCAAAGAATAGACTGTCAGCACGTGAACTTTTGGAACATCCTTTCATCAAGATGTATGAAGATAAGGATATTGATCTATCATCTTACTTCAATGATGCAGGATCTCCACTTGCAACTTTCTAA

Protein sequence

MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF
Homology
BLAST of Cucsat.G7501 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 519.2 bits (1336), Expect = 3.6e-146
Identity = 251/354 (70.90%), Postives = 293/354 (82.77%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           M+KGGFSNNL L + +  E QSI  FLTQSGTFKDGDL VN+DGVRI+SQ E E   PIK
Sbjct: 1   MKKGGFSNNLKLAIPVAGE-QSITKFLTQSGTFKDGDLRVNKDGVRIISQLEPEVLSPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           P DDQLSL+D+D++KVIGKG+ G VQLVQHKWT QFFALKVIQ+ I+E+ RK IAQELKI
Sbjct: 61  PADDQLSLSDLDMVKVIGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQS+QCP +V  YQSFYDNG+I +ILEYMDGGSLADFLK VK I + YL+A+ +QVL+GL
Sbjct: 121 NQSSQCPNLVTSYQSFYDNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
            YLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVS +M NT   ANTFVGTYNYMSPERI
Sbjct: 181 IYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VG  Y NKSDIWSLGL++LECATGKFPY+PP Q+  W + +ELMEAIV+  PP+ P+  F
Sbjct: 241 VGNKYGNKSDIWSLGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLAT 355
           +PE  SFIS C+Q DP +R SA+EL+EHPF+  Y+   I+L+SYF DAGSPLAT
Sbjct: 301 SPELSSFISTCLQKDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLAT 353

BLAST of Cucsat.G7501 vs. ExPASy Swiss-Prot
Match: Q94A06 (Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK1 PE=1 SV=2)

HSP 1 Score: 488.8 bits (1257), Expect = 5.2e-137
Identity = 237/341 (69.50%), Postives = 281/341 (82.40%), Query Frame = 0

Query: 16  LPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQLSLADIDIIK 75
           LP  +QSI+ FLTQSGTFKDGDL VN+DG++ VS SE  APPPI+P D+QLSLAD+++IK
Sbjct: 13  LPPLEQSISKFLTQSGTFKDGDLRVNKDGIQTVSLSEPGAPPPIEPLDNQLSLADLEVIK 72

Query: 76  VIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYVVVCYQS 135
           VIGKG+ G VQLV+HK T QFFALKVIQ+  EES  + I+QEL+IN S+QCPY+V CYQS
Sbjct: 73  VIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPYLVSCYQS 132

Query: 136 FYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHIIHRDLK 195
           FY NG + IILE+MDGGSLAD LKKV K+ E  L+A+CK+VL+GL Y+HHER IIHRDLK
Sbjct: 133 FYHNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHERRIIHRDLK 192

Query: 196 PSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKSDIWSLG 255
           PSNLLINHRGEVKITDFGVS I+ +T   AN+FVGTY YMSPERI G  Y NKSDIWSLG
Sbjct: 193 PSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYSNKSDIWSLG 252

Query: 256 LILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFISACVQTD 315
           L+LLECATGKFPY+PP    GW + YEL++AIVE  PP AP++ F+PEFCSFIS CVQ D
Sbjct: 253 LVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCSFISQCVQKD 312

Query: 316 PKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGS---PLA 354
           P++R SA+ELLEH F+KM+ED D +LS+YF DAGS   PLA
Sbjct: 313 PRDRKSAKELLEHKFVKMFEDSDTNLSAYFTDAGSLIPPLA 353

BLAST of Cucsat.G7501 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 2.3e-124
Identity = 208/332 (62.65%), Postives = 269/332 (81.02%), Query Frame = 0

Query: 12  LKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPP--IKPTDDQLSLA 71
           LKL++P ++  +  FLT SGTFKDG+L +N+ G++++S+   + P    +K  D QLS+ 
Sbjct: 10  LKLSVPAQETPVDKFLTASGTFKDGELRLNQRGLQLISEETADEPQSTNLKVEDVQLSMD 69

Query: 72  DIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQSAQCPYV 131
           D+++I+VIGKG+GG VQLV+HKW    +ALK IQM I+E+ RKQI QELKINQ+ Q  ++
Sbjct: 70  DLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELKINQATQNAHI 129

Query: 132 VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHHERHI 191
           V+C+QSFY NG IY++LEYMD GSLAD +K+VK I EPYLA LCKQVL+GL YLHHERH+
Sbjct: 130 VLCHQSFYHNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLLYLHHERHV 189

Query: 192 IHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGYDNKS 251
           IHRD+KPSNLL+N +GEVKITDFGVSA++ ++  + +TFVGTYNYM+PERI G  YD KS
Sbjct: 190 IHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERISGSSYDYKS 249

Query: 252 DIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFCSFIS 311
           DIWSLGL++LECA G+FPY  P +  GW++FYEL+EAIV+  PPSAPADQF+PEFC+FIS
Sbjct: 250 DIWSLGLVILECAIGRFPYI-PSEGEGWLSFYELLEAIVDQPPPSAPADQFSPEFCAFIS 309

Query: 312 ACVQTDPKNRLSARELLEHPFIKMYEDKDIDL 342
           +C+Q DP  R+SA ELL HPFIK +EDKD+DL
Sbjct: 310 SCIQKDPAERMSASELLNHPFIKKFEDKDLDL 340

BLAST of Cucsat.G7501 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.8e-121
Identity = 204/342 (59.65%), Postives = 270/342 (78.95%), Query Frame = 0

Query: 3   KGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPT 62
           KGG   N  L++N+PKE+ +I+ ++T SGTF+DGD+++N++G+R+VSQ++   P      
Sbjct: 9   KGG---NSALRVNVPKEN-AISDYVTASGTFQDGDIMLNKEGIRVVSQTQTSTP----AA 68

Query: 63  DDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQ 122
           +  +SL+D++ +KV+GKG+GG VQLV+HKWT + +ALKVI M IEE+ RKQI QELKIN 
Sbjct: 69  NGNISLSDLETVKVLGKGSGGVVQLVRHKWTNETYALKVIHMNIEETTRKQIVQELKINH 128

Query: 123 SAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKV--KKIEEPYLAALCKQVLKGL 182
           ++QCPYVV+CY +FY+NG I I+LEYMDGGSLAD +K++  K I+EP L  +CKQVL GL
Sbjct: 129 ASQCPYVVICYHAFYNNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVVCKQVLLGL 188

Query: 183 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 242
            YLH  RHIIHRD+KPSNLL+NH+GEVKI+DFGVSA++ N+    +TFVGT  YMSPER+
Sbjct: 189 MYLHQTRHIIHRDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTCTYMSPERV 248

Query: 243 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 302
           +G  Y   SDIWSLGL LLECA GK+PY PPG + GW+NFYEL++ IV+  PP APADQF
Sbjct: 249 LGGTYGFDSDIWSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPPPVAPADQF 308

Query: 303 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLS 343
           +PEFCSFISAC+Q DPK R +A ELL HPF+  Y++++  L+
Sbjct: 309 SPEFCSFISACLQKDPKCRPTAAELLNHPFVNKYDEEEYGLA 342

BLAST of Cucsat.G7501 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.7e-119
Identity = 204/336 (60.71%), Postives = 259/336 (77.08%), Query Frame = 0

Query: 7   SNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIKPTDDQL 66
           SN   LKL++P ++  I++FLT SGTF DGD L+N+ G+R+ S  +       K  D ++
Sbjct: 6   SNLKQLKLSVPAQESPISSFLTASGTFHDGDFLLNQKGLRLTSDEKQSRQSDSKELDFEI 65

Query: 67  SLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKINQ-SAQ 126
           +  D++ +KVIGKG+GG VQLV+HKW  +FFA+KVIQM I+E  RKQI QELKINQ S+Q
Sbjct: 66  TAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQELKINQASSQ 125

Query: 127 CPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGLSYLHH 186
           CP+VVVCY SFY NG+  ++LEYMD GSLAD +++VK I EPYLA +CKQVL GL YLH+
Sbjct: 126 CPHVVVCYHSFYHNGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVVCKQVLLGLVYLHN 185

Query: 187 ERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERIVGEGY 246
           ERH+IHRD+KPSNLL+NH+GEVKI+DFGVSA + ++  + +TFVGTYNYMSPERI G  Y
Sbjct: 186 ERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTYNYMSPERISGSTY 245

Query: 247 DNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQFTPEFC 306
           D  SDIWSLG+ +LECA G+FPY          +FYEL+ AIVE  PP+AP+DQF+PEFC
Sbjct: 246 DYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPPPTAPSDQFSPEFC 305

Query: 307 SFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDL 342
           SF+SAC+Q DP  R S+ +LL HPFIK +EDKDIDL
Sbjct: 306 SFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDL 341

BLAST of Cucsat.G7501 vs. NCBI nr
Match: XP_004144014.1 (mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN66268.1 hypothetical protein Csa_006896 [Cucumis sativus])

HSP 1 Score: 723 bits (1866), Expect = 3.69e-263
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. NCBI nr
Match: XP_008450884.2 (PREDICTED: LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 712 bits (1838), Expect = 6.85e-259
Identity = 348/355 (98.03%), Postives = 353/355 (99.44%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKE+QSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEEQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK I+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKXIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTY+EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYQEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQD GWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDEGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYED+DIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDRDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. NCBI nr
Match: XP_038880098.1 (mitogen-activated protein kinase kinase 2-like [Benincasa hispida] >XP_038880099.1 mitogen-activated protein kinase kinase 2-like [Benincasa hispida])

HSP 1 Score: 699 bits (1803), Expect = 1.48e-253
Identity = 344/355 (96.90%), Postives = 348/355 (98.03%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKI+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +T  EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTSGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGE YDNKSDIWSLGLILLECATGKFPYSPP QDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGERYDNKSDIWSLGLILLECATGKFPYSPPEQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQTDPK+RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQTDPKDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. NCBI nr
Match: XP_023516502.1 (mitogen-activated protein kinase kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688 bits (1775), Expect = 2.75e-249
Identity = 335/355 (94.37%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP+EDQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQEDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. NCBI nr
Match: XP_022961151.1 (mitogen-activated protein kinase kinase 2 [Cucurbita moschata] >KAG7023678.1 Mitogen-activated protein kinase kinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687 bits (1772), Expect = 7.87e-249
Identity = 334/355 (94.08%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. ExPASy TrEMBL
Match: A0A0A0LWW3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589750 PE=3 SV=1)

HSP 1 Score: 723 bits (1866), Expect = 1.79e-263
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. ExPASy TrEMBL
Match: A0A1S3BQA4 (LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103492345 PE=3 SV=1)

HSP 1 Score: 712 bits (1838), Expect = 3.31e-259
Identity = 348/355 (98.03%), Postives = 353/355 (99.44%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLPKE+QSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPKEEQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK I+EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKXIQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTY+EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYQEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQD GWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDEGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYED+DIDLSSYFNDAGSPLATF
Sbjct: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDRDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. ExPASy TrEMBL
Match: A0A6J1H9J6 (mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC111461750 PE=3 SV=1)

HSP 1 Score: 687 bits (1772), Expect = 3.81e-249
Identity = 334/355 (94.08%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. ExPASy TrEMBL
Match: A0A6J1JJS8 (mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485219 PE=3 SV=1)

HSP 1 Score: 686 bits (1769), Expect = 1.09e-248
Identity = 333/355 (93.80%), Postives = 346/355 (97.46%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFSNNLNLKLNLP++DQSIA FLTQSGTFKDGDLLVNRDGVRIVS+SEVEAPPPIK
Sbjct: 1   MRKGGFSNNLNLKLNLPQDDQSIAKFLTQSGTFKDGDLLVNRDGVRIVSKSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+VVCYQSFYDNGSIYIILEYMDGGSLADFLKKVK+++EPYLAALCKQVLKGL
Sbjct: 121 NQSAQCPYIVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKQVQEPYLAALCKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
           SYLHH+RHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +TY EANTFVGTYNYMSPERI
Sbjct: 181 SYLHHDRHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTYGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           +GE YDNKSDIWSLGLI LECATGKFPYSPP Q+GGWVNFYELMEAIVEGEPPSAPADQF
Sbjct: 241 IGEKYDNKSDIWSLGLIFLECATGKFPYSPPEQEGGWVNFYELMEAIVEGEPPSAPADQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FCSFISACVQ DP++RLSARELLEHPFIKMYED DIDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCSFISACVQKDPRDRLSARELLEHPFIKMYEDTDIDLSSYFNDAGSPLATF 355

BLAST of Cucsat.G7501 vs. ExPASy TrEMBL
Match: A0A6J1D344 (mitogen-activated protein kinase kinase 2-like OS=Momordica charantia OX=3673 GN=LOC111016574 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 3.63e-242
Identity = 330/355 (92.96%), Postives = 339/355 (95.49%), Query Frame = 0

Query: 1   MRKGGFSNNLNLKLNLPKEDQSIATFLTQSGTFKDGDLLVNRDGVRIVSQSEVEAPPPIK 60
           MRKGGFS NL  KLNLP+EDQSIA FLTQSGTF+DGDLLVNRDGVRIVSQSEVEAPPPIK
Sbjct: 1   MRKGGFSTNL--KLNLPQEDQSIAKFLTQSGTFRDGDLLVNRDGVRIVSQSEVEAPPPIK 60

Query: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120
           PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI
Sbjct: 61  PTDDQLSLADIDIIKVIGKGNGGTVQLVQHKWTAQFFALKVIQMKIEESHRKQIAQELKI 120

Query: 121 NQSAQCPYVVVCYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIEEPYLAALCKQVLKGL 180
           NQSAQCPY+V+CYQSFYDNGSIYIILEYMDGGSLADFLKKVKKI+EPYLAA+CKQVLKGL
Sbjct: 121 NQSAQCPYIVICYQSFYDNGSIYIILEYMDGGSLADFLKKVKKIQEPYLAAICKQVLKGL 180

Query: 181 SYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMENTYEEANTFVGTYNYMSPERI 240
            YLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIM +T  EANTFVGTYNYMSPERI
Sbjct: 181 LYLHHERHIIHRDLKPSNLLINHRGEVKITDFGVSAIMASTSGEANTFVGTYNYMSPERI 240

Query: 241 VGEGYDNKSDIWSLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPADQF 300
           VG  YDNKSDIW LGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAP+DQF
Sbjct: 241 VGARYDNKSDIWCLGLILLECATGKFPYSPPGQDGGWVNFYELMEAIVEGEPPSAPSDQF 300

Query: 301 TPEFCSFISACVQTDPKNRLSARELLEHPFIKMYEDKDIDLSSYFNDAGSPLATF 355
           TP FC+FISACVQ DPK RLSARELLEHPFIKMYED  IDLSSYFNDAGSPLATF
Sbjct: 301 TPNFCAFISACVQQDPKVRLSARELLEHPFIKMYEDTAIDLSSYFNDAGSPLATF 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7U93.6e-14670.90Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q94A065.2e-13769.50Mitogen-activated protein kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q5QN752.3e-12462.65Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
A9RWC96.8e-12159.65Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
Q9FJV01.7e-11960.71Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
XP_004144014.13.69e-263100.00mitogen-activated protein kinase kinase 2 [Cucumis sativus] >KGN66268.1 hypothet... [more]
XP_008450884.26.85e-25998.03PREDICTED: LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like [... [more]
XP_038880098.11.48e-25396.90mitogen-activated protein kinase kinase 2-like [Benincasa hispida] >XP_038880099... [more]
XP_023516502.12.75e-24994.37mitogen-activated protein kinase kinase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022961151.17.87e-24994.08mitogen-activated protein kinase kinase 2 [Cucurbita moschata] >KAG7023678.1 Mit... [more]
Match NameE-valueIdentityDescription
A0A0A0LWW31.79e-263100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G589... [more]
A0A1S3BQA43.31e-25998.03LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase 2-like OS=Cucumis m... [more]
A0A6J1H9J63.81e-24994.08mitogen-activated protein kinase kinase 2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJS81.09e-24893.80mitogen-activated protein kinase kinase 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1D3443.63e-24292.96mitogen-activated protein kinase kinase 2-like OS=Momordica charantia OX=3673 GN... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 146..159
score: 49.12
coord: 183..201
score: 35.52
coord: 249..271
score: 34.02
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 71..308
e-value: 1.8E-66
score: 236.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 73..308
e-value: 2.5E-63
score: 213.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 71..311
score: 43.749691
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 151..311
e-value: 2.7E-51
score: 176.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 47..150
e-value: 6.2E-29
score: 102.1
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 71..271
e-value: 1.5E-29
score: 100.7
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 59..269
e-value: 4.7E-34
score: 115.8
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 9..310
NoneNo IPR availablePANTHERPTHR24361:SF759MAP KINASE KINASEcoord: 9..310
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 69..310
e-value: 1.79393E-142
score: 400.429
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 77..100
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 55..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G7501.T2Cucsat.G7501.T2mRNA
Cucsat.G7501.T3Cucsat.G7501.T3mRNA
Cucsat.G7501.T1Cucsat.G7501.T1mRNA
Cucsat.G7501.T6Cucsat.G7501.T6mRNA
Cucsat.G7501.T7Cucsat.G7501.T7mRNA
Cucsat.G7501.T4Cucsat.G7501.T4mRNA
Cucsat.G7501.T5Cucsat.G7501.T5mRNA
Cucsat.G7501.T9Cucsat.G7501.T9mRNA
Cucsat.G7501.T8Cucsat.G7501.T8mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity