Cucsat.G734 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G734
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	endochitinase-like
Location	ctg1: 323726 .. 326892 (+)
RNA-Seq Expression	Cucsat.G734
Synteny	Cucsat.G734

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATGAAACTTCAAGTTTAGGATAGAATCGGTTTGACAAAAGAAAAAGGGCGAAATTACGAACACTTATTCATGTAATTCGATAAACATGAAAACTGCACAGAAGGAAAAAGATAGATAGAAGAATTTCATGGTAAAATAATGGCTGATTTCCTTAAAAAAATTTCAAAAATGGATAACTTTTCAACGCTGCCTTTCCTTCTTTTTCCCAATACTTCCAAACGAACCCATAAGTCAACAAACAAATCACTCGCAGCGCCATTCGATGACGAAAATCTACACCTCTTTCATAACCCTAACTTTCGTTCTCTTGACGACACCGGCGACGTCATCGGCGCAGTCATGTGGCGGCGGAGAATCGGCTGACGCCATGTGTCCAAAAGGTCTCTGCATCGCCGGGTCGGGGTTATGTGGATCTGGATTTCTGAGGCGATGCGGTTCGGATCTAACATTTGGGAACCCTAACGAGGGAATGGGGGGCGTAGTTAGCGCATCGGAATTCGACGGAATGCTTAAACATCGAAACGATGGGAGTTGCGAGGGGAACGGATTTTATAGCTATGAGGCTTTTGTGGTTGCGGCGGCGGAGTTCGATGGCTTCGGCACCACTGGGAATTTGAGTACAAGGAAGAGGGAGGTCGCTGCTTTCTTGGCTCAAACATCTTACGAGACCACAGGTTCATTATCATTTTCTTTCTAATTATTTTCTCTTCTAATTACTATTAATTCATTTTAAGTTTTTTCCTTTTTCTTTGTTCCATTAGTTTAAACTTTCAACATTAAAATAACCATTGTTTTTAGTCCCTATACTTTCATTTTTAACGGTCTTACTTTAAATAAATCCAAGACTAGTTTATTGTTTCATTTCTTTTTATTTTTTTAATTAAATTTTTACTTTAAAATTTGAAAACATATTCACACTTTAACTTATTATTATTATTAACCAATTTTAATAAAAAATAATTTTAAAAGATTAATTTAAATAAATGCAAGAACAAAAAAAAAAAAAAAAAGAAATAAAGAACTAAAAGTATAAGAAAGACAAACACTATACCTTTAGTACTATTTAATGGTTAAGTCTTGAGTTTAACGCTTCTTTTTCTAGGTAAATATCATGTCATCATTGTTTTTTTATTCATTTCATTTGAGTTGTACTCTTAATACTATACTTCAAATCAATTTAAATTTAAATTTAATCTCTTAATAAAATATAAAAAAATTATTGAAAACAAAATATTTCACAAAATATTTACCCTTCCTATTATCGAAAACAAAATATTTATCTAACAAATTTAAACTTTATATTGTCTTTTAGTGATTTTGTTCCTTCTATAAAGTATAAATATTTTGACATTTTTTATATTTAAAAGAAAACCTATAAATTTTAAAAAAATTATCAAAAATAGCAAATTTATTAAAATATTTACTAACATATAACAAAATTTTCAAATTCTATCAATATTACATATTTGACCGACAATAATATCCTTCTATCAACTACATTGATAAAAAGTCTACCGATTATTTCTATCATTGATATAATTAAAAAAATTTGTTACAACTTATAAATATTTTAATTTATTTTGGTGTTTTTTAAAGGTACTTAAATTTTAATCAAAATTGTTAAAATAAGAATAACAAAATTTTACAAGAAATATTAAAAGAGAGTATTTTTTAAAAAAGAAAAAATCAACAACAAACATAGCAATGACTAATCTGAAGATTTTATTAACAGTATAAAACACAAAGGATGGAAACTAAAAATAGCGTTTTGACCTTTTTTTATTTTAAAAAGATTAATCTCTTTGAAGATTTGTTTCTTTCTAGTAGGATTTAATCGATCTTGACTAGACATGATAACTTTCTTTTATAAACTCCAAAAAGTAAATAATAATAATAATATAAAAAAAGACTCAGTATTTTATACAACTATGTGATTATATATGGAAGATGATATCAGACCATAAGCATGTCAATAGAAAATCATATTAAATACTATCAAGCATATCAGCAACAGATCTACAATTAGTATTTAAATAGAGTGAAATCAAATTTTTTAATTTTTTAAAAAAAGAGAGATTAAAAAAAATACTTTTAACCACTTAGTAAAACATCTTATGATATTAAATAAGAAATAACAATTTATTTCAACATAAACATTTAGGAGGATGGGCAACTGCACCAGATGGTCCATATGCATGGGGGTATTGCTATATCAATGCAACCCAAAAAGATCAATTTTGTGTTCCATCACCCACTTGGCCTTGTTCATCTGGCAAGTTATATTATGGCCGAGGTCCCTTCCAAATCGCCTAGTAACTTCTCTCTTCTTTCTTTTTACTTCATACATATTCATAAGCTTTTGATTTTTAATTTCTACATTTGCATCATTGAAAATATGATTTTGTTTTGGTTGATTAGTAACTTTAACTATGGGGCTGCTGGAAAAGCAATTGGAGTGGATCTCTTAAACAATCCTGATCTAGTGGCTACTAATCCAACTATATCATTCAAGACTGCCTTATGGTTTTGGATGACTTCTCAAGGAGACAAACCATCGTGTCATGATGTTATGACAGGTAAATGGGTACCATCTAATGAAGATGTATCGGCTAAGAGATTACCTGGTTATGGTCTAACCATCAACATAATTAATGGTAATAATGAATGTGGTAAAGGCGCCGATGCTCGAGCAGCTGATCGGCTTGGTTTCTACCAAAGGTATTGCGATGTATTGAACATCCAATATGGTGACAACTTTAATTGTGTCAATCAAAAGCCGTTTACCTATGAAGAATGATTCATAAGAAGTTGCACGAGAGATACAGAGACTATAATTGTTTAATTTGCTAATCTTTCCCACTTTTCCTTGGAAAAGCTTCTTATTAGTAGCAAGGAAAATATTTTTCCATACCATCTCAGTTTATTAGTTTATACAACATTTATTTTTAAATAGTATGAAATCCATGAAATGAAACAATACATAACCAAATAGAACATAGCAGCCTCCAACACATAGAAAGCAAAATGATATTAAGCATTTCAGAGAGATTTTAAGTGAATATTAAAGTATGTTTGGGAATGATTTCAAAAATGATAAAAGTGATTTTTAACCTTTCTAGTATCATCTCCAAATATACAATAAGCATTTCAAGAATTGTGTAATT

Coding sequence (CDS)

ATGGTCCATATGCATGGGGGTATTGCTATATCAATGCAACCCAAAAAGATCAATTTTGTGTTCCATCACCCACTTGGCCTTGTTCATCTGGCAAGTTATATTATGGCCGAGGTCCCTTCCAAATCGCCTAGTAACTTCTCTCTTCTTTCTTTTTACTTCATACATATTCATAAGCTTTTGATTTTTAATTTCTACATTTGCATCATTGAAAATATGATTTTGTTTTGGTTGATTAGTAACTTTAACTATGGGGCTGCTGGAAAAGCAATTGGAGTGGATCTCTTAAACAATCCTGATCTAGTGGCTACTAATCCAACTATATCATTCAAGACTGCCTTATGGTTTTGGATGACTTCTCAAGGAGACAAACCATCGTGTCATGATGTTATGACAGGTAAATGGGTACCATCTAATGAAGATGTATCGGCTAAGAGATTACCTGGTTATGGTCTAACCATCAACATAATTAATGGTAATAATGAATGTGGTAAAGGCGCCGATGCTCGAGCAGCTGATCGGCTTGGTTTCTACCAAAGGTATTGCGATGTATTGAACATCCAATATGGTGACAACTTTAATTGTGTCAATCAAAAGCCGTTTACCTATGAAGAATGA

Protein sequence

MVHMHGGIAISMQPKKINFVFHHPLGLVHLASYIMAEVPSKSPSNFSLLSFYFIHIHKLLIFNFYICIIENMILFWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKPFTYEE

Homology

[Close]

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot
Match: P21226 (Endochitinase A2 OS=Pisum sativum OX=3888 GN=CHI2 PE=1 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 6.8e-49
Identity = 83/121 (68.60%), Postives = 97/121 (80.17%), Query Frame = 0


Query: 80  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNE 139
           N+NYG  G+AIGVDLLNNPDLVAT+P ISFKTALWFWMT Q  KPSCHDV+TG W PS+ 
Sbjct: 187 NYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVITGGWTPSSA 246

Query: 140 DVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKP 199
           D +A RLPGYG   NIING  ECG+G D+R  DR+GFY+RYCD+  I YGDN +C +Q+P
Sbjct: 247 DRAAGRLPGYGTVTNIINGGLECGRGQDSRVQDRIGFYKRYCDIFGIGYGDNLDCYSQRP 306

Query: 200 F 201
           F
Sbjct: 307 F 307

[Close]

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot
Match: Q9FRV0 (Basic endochitinase C OS=Secale cereale OX=4550 GN=rscc PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.8e-48
Identity = 83/124 (66.94%), Postives = 99/124 (79.84%), Query Frame = 0


Query: 77  LISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVP 136
           L  N+NYG AG+AIGVDLL NPDLVAT+PT+SFKTALWFWMT+Q  KPS H V+TGKW P
Sbjct: 142 LSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTALWFWMTAQAPKPSSHAVITGKWSP 201

Query: 137 SNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVN 196
           S  D +A R PG+G+  NIING  ECG G D+R ADR+GFY+RYCD+L + YGDN +C N
Sbjct: 202 SGADRAAGRAPGFGVITNIINGGLECGHGQDSRVADRIGFYKRYCDILGVGYGDNLDCYN 261

Query: 197 QKPF 201
           Q+PF
Sbjct: 262 QRPF 265

[Close]

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot
Match: Q688M5 (Chitinase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht9 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 81/121 (66.94%), Postives = 99/121 (81.82%), Query Frame = 0


Query: 80  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNE 139
           N+NYG AG+AIG DLLNNP+LVA++P +SFKTALWFWMT Q  KPSCHDV+TG+W PS+ 
Sbjct: 206 NYNYGPAGEAIGEDLLNNPELVASDPVVSFKTALWFWMTPQSPKPSCHDVITGQWTPSSG 265

Query: 140 DVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKP 199
           D++A R+PGYG+  NIING  ECG G D R A+R+GFYQRYCDVL I YG N +C +Q+P
Sbjct: 266 DIAAGRVPGYGVITNIINGGLECGFGPDDRVANRIGFYQRYCDVLGIGYGSNLDCYDQRP 325

Query: 200 F 201
           F
Sbjct: 326 F 326

[Close]

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot
Match: P86473 (Endochitinase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04863 PE=1 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 3.7e-47
Identity = 82/121 (67.77%), Postives = 96/121 (79.34%), Query Frame = 0


Query: 80  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNE 139
           N+NYG AGKA+GVDLLNNPDLVAT+P ISFKTALWFWMT Q  KPSCHDV+TG+W PS  
Sbjct: 193 NYNYGPAGKALGVDLLNNPDLVATDPAISFKTALWFWMTPQSPKPSCHDVITGRWTPSAA 252

Query: 140 DVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKP 199
           D SA RLPG+G+  NIING  ECG G+D+R  DR+GFY+RYC +  I  GDN +C NQ+ 
Sbjct: 253 DRSAGRLPGFGVITNIINGGLECGHGSDSRVQDRIGFYKRYCQIFGIGTGDNLDCGNQRS 312

Query: 200 F 201
           F
Sbjct: 313 F 313

[Close]

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot
Match: Q9FRV1 (Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 8.3e-47
Identity = 79/124 (63.71%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 77  LISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVP 136
           L  N+NYG AG+AIGVDLL NPDLVAT+PT+SFKTA+WFWMT+Q  KPS H V+TG+W P
Sbjct: 197 LSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPKPSSHAVITGQWSP 256

Query: 137 SNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVN 196
           S  D +A R+PG+G+  NI+NG  ECG G D+R ADR+GFY+RYCD+L + YG+N +C N
Sbjct: 257 SGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILRVGYGNNLDCYN 316

Query: 197 QKPF 201
           Q+PF
Sbjct: 317 QRPF 320

[Close]

BLAST of Cucsat.G734 vs. NCBI nr
Match: XP_004146167.1 (basic endochitinase C [Cucumis sativus] >KGN55621.1 hypothetical protein Csa_010177 [Cucumis sativus])

HSP 1 Score: 274 bits (701), Expect = 4.29e-89
Identity = 126/130 (96.92%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW
Sbjct: 184 FQIAYNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 243

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC
Sbjct: 244 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 303

Query: 195 VNQKPFTYEE 204
           VNQKPFTYEE
Sbjct: 304 VNQKPFTYEE 313

[Close]

BLAST of Cucsat.G734 vs. NCBI nr
Match: BAB40818.1 (endochitinase MCHT-3, partial [Cucumis melo])

HSP 1 Score: 246 bits (628), Expect = 4.09e-80
Identity = 111/127 (87.40%), Postives = 119/127 (93.70%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 40  FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 99

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGADARAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 100 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADARAADRLGFYQRYCDVLNIQYGGNLNC 159

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 160 VDQKPFT 166

[Close]

BLAST of Cucsat.G734 vs. NCBI nr
Match: KAA0052928.1 (basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase C [Cucumis melo var. makuwa])

HSP 1 Score: 244 bits (624), Expect = 1.87e-77
Identity = 110/127 (86.61%), Postives = 118/127 (92.91%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 185 FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 244

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGAD RAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 245 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNC 304

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 305 VDQKPFT 311

[Close]

BLAST of Cucsat.G734 vs. NCBI nr
Match: XP_008448502.1 (PREDICTED: basic endochitinase C [Cucumis melo])

HSP 1 Score: 244 bits (624), Expect = 4.96e-77
Identity = 110/127 (86.61%), Postives = 118/127 (92.91%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 217 FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 276

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGAD RAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 277 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNC 336

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 337 VDQKPFT 343

[Close]

BLAST of Cucsat.G734 vs. NCBI nr
Match: XP_038902998.1 (basic endochitinase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 228 bits (582), Expect = 3.62e-71
Identity = 100/127 (78.74%), Postives = 112/127 (88.19%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  NFNYGAAGKAIGVDL+NNPDLVATNP ISFKTALWFWMTSQG+KPSCHDVMTGKW
Sbjct: 180 FQITYNFNYGAAGKAIGVDLINNPDLVATNPIISFKTALWFWMTSQGNKPSCHDVMTGKW 239

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           +PS+ED+SAKR PGYGLTIN+I G NEC    DARA DR+GF+QRYCD+LNI YGDN +C
Sbjct: 240 IPSDEDISAKRFPGYGLTINLIGGINECSSDTDARAVDRVGFFQRYCDILNIPYGDNLDC 299

Query: 195 VNQKPFT 201
            +QKPFT
Sbjct: 300 FDQKPFT 306

[Close]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL
Match: A0A0A0L646 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1)

HSP 1 Score: 274 bits (701), Expect = 2.08e-89
Identity = 126/130 (96.92%), Postives = 127/130 (97.69%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW
Sbjct: 184 FQIAYNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 243

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC
Sbjct: 244 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 303

Query: 195 VNQKPFTYEE 204
           VNQKPFTYEE
Sbjct: 304 VNQKPFTYEE 313

[Close]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL
Match: Q9AVA7 (Endochitinase MCHT-3 (Fragment) OS=Cucumis melo OX=3656 GN=mcht-3 PE=2 SV=1)

HSP 1 Score: 246 bits (628), Expect = 1.98e-80
Identity = 111/127 (87.40%), Postives = 119/127 (93.70%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 40  FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 99

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGADARAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 100 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADARAADRLGFYQRYCDVLNIQYGGNLNC 159

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 160 VDQKPFT 166

[Close]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL
Match: A0A5A7UCL1 (Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00370 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 9.05e-78
Identity = 110/127 (86.61%), Postives = 118/127 (92.91%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 185 FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 244

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGAD RAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 245 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNC 304

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 305 VDQKPFT 311

[Close]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL
Match: A0A1S3BKQ5 (basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 2.40e-77
Identity = 110/127 (86.61%), Postives = 118/127 (92.91%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  N+NYG AG+AIGVDLLNNPDLVATNPTISFKTA+WFWMTS G+ PSCH+VMTGKW
Sbjct: 217 FQITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKW 276

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNEDVSAKRLPGYGLTIN+INGNNECGKGAD RAADRLGFYQRYCDVLNIQYG N NC
Sbjct: 277 VPSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNC 336

Query: 195 VNQKPFT 201
           V+QKPFT
Sbjct: 337 VDQKPFT 343

[Close]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL
Match: A0A6J1E705 (basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV=1)

HSP 1 Score: 227 bits (578), Expect = 7.06e-71
Identity = 102/126 (80.95%), Postives = 114/126 (90.48%), Query Frame = 0


Query: 75  FWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKW 134
           F +  NFNYGAAG+AIGVDL+NNPDLVATNPTISFKTALWFWMT QG+KPSCHDVMTGKW
Sbjct: 182 FQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMTGKW 241

Query: 135 VPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNC 194
           VPSNED+SAKRLPGYGLTIN+IN  +EC  G D RAADR+GFY+RYCD+LNIQYGDN +C
Sbjct: 242 VPSNEDISAKRLPGYGLTINLIN-VSECVNGTDDRAADRIGFYKRYCDILNIQYGDNLDC 301

Query: 195 VNQKPF 200
           V Q+PF
Sbjct: 302 VGQQPF 306

[Close]

BLAST of Cucsat.G734 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 175.3 bits (443), Expect = 5.2e-44
Identity = 77/129 (59.69%), Postives = 95/129 (73.64%), Query Frame = 0


Query: 72  MILFWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMT 131
           M L W   N+NYG  G+AIGVDLLNNPDLVA +  I+FK A+WFWMT+Q  KPSCH V+ 
Sbjct: 201 MQLSW---NYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIA 260

Query: 132 GKWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDN 191
           G+W PS+ D +A RLPGYG+  NIING  ECG+G D R ADR+GFYQRYC++  +  G N
Sbjct: 261 GQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPGGN 320

Query: 192 FNCVNQKPF 201
            +C NQ+ F
Sbjct: 321 LDCYNQRSF 326

[Close]

BLAST of Cucsat.G734 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 154.1 bits (388), Expect = 1.2e-37
Identity = 66/121 (54.55%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 80  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNE 139
           N+NYG AG+A+G D L NP+ V+ N  I+F+TALWFWMT Q  KPSCHDVM GK+ P+  
Sbjct: 151 NYNYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRPTAA 210

Query: 140 DVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKP 199
           D++A R  G+GLT NIING  ECG   D R  DR+GF+QRY  +  +  G N +C NQ+P
Sbjct: 211 DLAANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCENQRP 270

Query: 200 F 201
           +
Sbjct: 271 Y 271

[Close]

BLAST of Cucsat.G734 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.1e-37
Identity = 65/122 (53.28%), Postives = 84/122 (68.85%), Query Frame = 0


Query: 80  NFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTGKWVPSNE 139
           N+NYG AG+A+G D L NP+LVA N  ++FKTALWFWMT Q  KPSCH+VM  ++ P+  
Sbjct: 159 NYNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKA 218

Query: 140 DVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNFNCVNQKP 199
           D +A R  GYGL  NIING  ECG   D R  DR+G++QRY  +  +  G N +C NQ+P
Sbjct: 219 DRAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRP 278

Query: 200 FT 202
           F+
Sbjct: 279 FS 280

[Close]

BLAST of Cucsat.G734 vs. TAIR 10
Match: AT1G05850.1 (Chitinase family protein )

HSP 1 Score: 109.0 bits (271), Expect = 4.6e-24
Identity = 50/133 (37.59%), Postives = 78/133 (58.65%), Query Frame = 0


Query: 74  LFWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTS-QGDKPSCHDVMTG 133
           ++W   NFNYGAAG+A+  DLLN+P+ +  N T++F+ A+W WMT  +  +PS HD+  G
Sbjct: 181 IYW---NFNYGAAGEALKADLLNHPEYIEQNATLAFQAAIWRWMTPIKRAQPSAHDIFVG 240

Query: 134 KWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQY---- 193
            W P+  D  +KR P +G T+N++ G   CG+G+     + +  Y  + D++ I      
Sbjct: 241 NWKPTKNDTLSKRGPTFGSTMNVLYGEYTCGQGSIDPMNNIISHYLYFLDLMGIGREDAG 300

Query: 194 -GDNFNCVNQKPF 201
             D  +C  QKPF
Sbjct: 301 PNDELSCAEQKPF 310

[Close]

BLAST of Cucsat.G734 vs. TAIR 10
Match: AT3G16920.1 (chitinase-like protein 2 )

HSP 1 Score: 108.6 bits (270), Expect = 6.0e-24
Identity = 51/135 (37.78%), Postives = 82/135 (60.74%), Query Frame = 0


Query: 74  LFWLISNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMT-SQGDKPSCHDVMTG 133
           ++W   N+NYG  G+A+ VDLL++P+ +  N T++F+ A+W WMT  +   PS HDV  G
Sbjct: 204 VYW---NYNYGQTGEALKVDLLSHPEYLENNATLAFQAAIWRWMTPPKKHLPSAHDVFVG 263

Query: 134 KWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRL-GFYQRYCDVLNI---QY 193
           KW P+  D +AKR PG+G TIN++ G+  C  G D    + +   Y  Y D++ +   + 
Sbjct: 264 KWKPTKNDTAAKRTPGFGATINVLYGDQICNSGFDNDEMNNIVSHYLYYLDLIGVGREEA 323

Query: 194 G--DNFNCVNQKPFT 202
           G  +  +C +Q+PF+
Sbjct: 324 GPHEKLSCADQEPFS 335

The following BLAST results are available for this feature:

BLAST of Cucsat.G734 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P21226	6.8e-49	68.60	Endochitinase A2 OS=Pisum sativum OX=3888 GN=CHI2 PE=1 SV=2	[more]
Q9FRV0	5.8e-48	66.94	Basic endochitinase C OS=Secale cereale OX=4550 GN=rscc PE=1 SV=1	[more]
Q688M5	1.3e-47	66.94	Chitinase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht9 PE=2 SV=1	[more]
P86473	3.7e-47	67.77	Endochitinase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04863...	[more]
Q9FRV1	8.3e-47	63.71	Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1	[more]

BLAST of Cucsat.G734 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146167.1	4.29e-89	96.92	basic endochitinase C [Cucumis sativus] >KGN55621.1 hypothetical protein Csa_010...	[more]
BAB40818.1	4.09e-80	87.40	endochitinase MCHT-3, partial [Cucumis melo]	[more]
KAA0052928.1	1.87e-77	86.61	basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase...	[more]
XP_008448502.1	4.96e-77	86.61	PREDICTED: basic endochitinase C [Cucumis melo]	[more]
XP_038902998.1	3.62e-71	78.74	basic endochitinase-like isoform X2 [Benincasa hispida]	[more]

BLAST of Cucsat.G734 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L646	2.08e-89	96.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1	[more]
Q9AVA7	1.98e-80	87.40	Endochitinase MCHT-3 (Fragment) OS=Cucumis melo OX=3656 GN=mcht-3 PE=2 SV=1	[more]
A0A5A7UCL1	9.05e-78	86.61	Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13...	[more]
A0A1S3BKQ5	2.40e-77	86.61	basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1	[more]
A0A6J1E705	7.06e-71	80.95	basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV...	[more]

BLAST of Cucsat.G734 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12500.1	5.2e-44	59.69	basic chitinase	[more]
AT1G02360.1	1.2e-37	54.55	Chitinase family protein	[more]
AT4G01700.1	2.1e-37	53.28	Chitinase family protein	[more]
AT1G05850.1	4.6e-24	37.59	Chitinase family protein	[more]
AT3G16920.1	6.0e-24	37.78	chitinase-like protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 156..212 e-value: 3.5E-110 score: 369.1
None	No IPR available	GENE3D	1.10.530.10		coord: 77..309 e-value: 3.5E-110 score: 369.1
None	No IPR available	PANTHER	PTHR22595:SF171	CHITINASE (CLASS IB) / HEVEIN	coord: 8..309
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 8..309
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..27 score: 5.0
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 74..303 e-value: 1.61772E-98 score: 287.789
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 73..303 e-value: 1.3E-113 score: 378.5
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 91..113
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 216..226
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 1..310 e-value: 1.1E-105 score: 351.2
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 71..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G734.T2	Cucsat.G734.T2	mRNA
Cucsat.G734.T1	Cucsat.G734.T1	mRNA
Cucsat.G734.T3	Cucsat.G734.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0000272	polysaccharide catabolic process
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding