Cucsat.G7295 (gene) Cucumber (B10) v3

BLAST of Cucsat.G7295 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.8e-187
Identity = 312/578 (53.98%), Postives = 433/578 (74.91%), Query Frame = 0

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY D+D++ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI++S+KAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAI 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS  + +L+KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
           A  AL  CKELMD+AI EL  S  +LG+F+  K+DE L+ +RIWLSATI++++TCLDGF 
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        ++P
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 243

Query: 242 DWANPGMRRLL-AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 301
            W +   RRLL A  S+VKP++VVA+DGSGQ+KTI EA++ VPK+K N T+V+HIK G+Y
Sbjct: 244 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 303

Query: 302 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 361
           +EYV V +++THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 421
           AG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 481
           FQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q C+     D + V++Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 541
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGA 579
           ++ +  + FTP ++++GD WI   GVPY+ GL    G+
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGS 568

BLAST of Cucsat.G7295 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 651.7 bits (1680), Expect = 7.6e-186
Identity = 313/582 (53.78%), Postives = 430/582 (73.88%), Query Frame = 0

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY DD+S+ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E+++S+KA+
Sbjct: 4   GY-DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVKAV 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS  + +L+KD R
Sbjct: 64  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
              AL  CKELMD+A+DEL  S  +LG+F+   LDE LI++RIWLSA I+++ETCL+GF 
Sbjct: 124 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E MKKALKT+++L+ NGLA++S++S+ + ++QIPG++ RRLL       + +P
Sbjct: 184 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 243

Query: 242 DWANPGMRRLL---AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAG 301
            W +   R+LL   AA S VKP++VVA+DGSGQ+KTI EA+  VPK K N T+V+HIKAG
Sbjct: 244 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 303

Query: 302 VYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFE 361
           +Y+EYV V KT++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 304 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 363

Query: 362 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 421
           NTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCT+SGTIDF+FGDAA
Sbjct: 364 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 423

Query: 422 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLG 481
           A+FQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q C+     D + V++  +++LG
Sbjct: 424 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 483

Query: 482 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGI 541
           RPWKEYSRTIIM ++I D +QP+GW PW GD+GL+T FY+E  N GPGS  + RV W GI
Sbjct: 484 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 543

Query: 542 KNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGA 581
           K ++ +  + FTP ++++GD WI   GVPY +GL     A A
Sbjct: 544 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAAA 573

BLAST of Cucsat.G7295 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 4.2e-152
Identity = 274/575 (47.65%), Postives = 381/575 (66.26%), Query Frame = 0

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETSDPATGNKSHEISSSMKAIKA 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I ++  A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANK-SVALNQLEKDPRA 125
           +C PTDYK+ CV SL     +S+ P +L++ GF   ++ I+ +  K SV L     + + 
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 SK-ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 185
           +K AL  C++LM+ A D+LK  ++    F I ++++ + D+R+WLS +I YQ+TC+D F 
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVL 245
            T    ++ M+K  KTS +L+SNGLAM++ IS++L E  + G++       R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHI 305
             D  P W  P  RRL+A    VK NVVVA DGSGQ+KTI EA++ VPK  N   +VI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYI 303

Query: 306 KAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDG-TPTFKTATVAVTAEHFMARD 365
           K GVY E V V K +TH+  IGDGP KT ITG+ N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIF 425
           IGFENTAGP+ HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCTVSGT+DFIF
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFR 485
           GD   + Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QNC        +PV+   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVK 545
           ++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y EY N GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYV 570
           W GIK ++P+ A+ FTP RFL+G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cucsat.G7295 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 510.4 bits (1313), Expect = 2.7e-143
Identity = 264/577 (45.75%), Postives = 370/577 (64.12%), Query Frame = 0

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS  +  +  + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVA--LNQLEKDPRASK 126
           PTD+K  CV SL     +S DP +L++ GFK  +K I  +  K+      + +K+P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTT 186
           A   C++LM  AID+LK  M+    F + +++  + D+R+WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS +LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAA----GSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQFKTI +A++ VPK  N   ++IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK G+Y+E V V K + H+  IGDGP KT+ITG+ NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFI 426
           +IG ENTAGP+  QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCTVSGT+DFI
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQF 486
           FGDA  I Q+C  +VRKP   Q C+VTA GR   R+ + L++  C        +P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ L+T +Y E+ N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVS 571
           KW GIK +TPQ A+ +T  RFL+GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Cucsat.G7295 vs. ExPASy Swiss-Prot
Match: Q42920 (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 2.1e-127
Identity = 226/449 (50.33%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 129 CKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTTGNAA 188
           C E++D+A+D +  S+  L +FD  KL E   D+++WL+ T+++Q+TCLDGFANTT  A 
Sbjct: 4   CNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTTKAG 63

Query: 189 EKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYPDWANPGM 248
           E M K LKTSM+LSSN + M+  +S +L        S  R L    L  D  P W N G 
Sbjct: 64  ETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRL----LSDDGIPSWVNDGH 123

Query: 249 RRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVYQEYVLVKK 308
           RRLLA G+ V+PN VVA+DGSGQFKT+ +A+  VP  KN   +VIH+KAGVY+E V V K
Sbjct: 124 RRLLAGGN-VQPNAVVAQDGSGQFKTLTDALKTVPP-KNAVPFVIHVKAGVYKETVNVAK 183

Query: 309 TLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTAGPQKHQA 368
            + ++ +IGDGP KT  TG+ N+ DG  T+ TAT  V   +FMA+DIGFENTAG  KHQA
Sbjct: 184 EMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQA 243

Query: 369 VALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAIFQSCTFLV 428
           VALRV AD+A+FYNC+M G+QDTLYV + RQFYRDC++SGTIDF+FG+   +FQ+C  + 
Sbjct: 244 VALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVC 303

Query: 429 RKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRPWKEYSRTI 488
           R P   QQC+VTA GR+++   SAL+ Q+  F     L  V  +  S+LGRPWK+YS+ +
Sbjct: 304 RLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVTPKV-SYLGRPWKQYSKVV 363

Query: 489 IMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKNITPQHAVD 548
           IM+S I  +  PEG++PW G     TC Y EYNN GPG+D + RVKW G+K +T   A +
Sbjct: 364 IMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAE 423

Query: 549 FTPGRFLK------GDRWIKPTGVPYVSG 572
           + PG+F +       D WI  +GVPY  G
Sbjct: 424 YYPGKFFEIVNATARDTWIVKSGVPYSLG 445

BLAST of Cucsat.G7295 vs. NCBI nr
Match: XP_004135775.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.1 hypothetical protein Csa_007353 [Cucumis sativus])

HSP 1 Score: 1172 bits (3031), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583
           ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583

BLAST of Cucsat.G7295 vs. NCBI nr
Match: XP_008450602.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >KAA0050920.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa] >TYK10268.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 1117 bits (2889), Expect = 0.0
Identity = 550/583 (94.34%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQEC+ASLKATGNNSSDP+ELVQAGFKAAMKLIQAAANKSV L QLEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAIDELKYSMNKLGEFDI+KLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGS+VKPNVVVAKDGSGQF+TIQ A+DQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEY+ +KKT+THLMLIGDGPKKTIITGNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS K   DL PVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGS+KSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTGGA AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAAAAH 583

BLAST of Cucsat.G7295 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1085 bits (2805), Expect = 0.0
Identity = 532/580 (91.72%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQECVASLKA GNNSSDPKELVQAGFKAAMKLIQAAANKSV L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA+DELKYSMNKLGEFDISKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQF+TIQEA+D+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+++KK+LTHLMLIGDGPKKTIITG+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFK  ADL PVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGA 580
           ITPQHAVDFT GRFL+GD WIK TGVPYVSG+TRT  A A
Sbjct: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 580

BLAST of Cucsat.G7295 vs. NCBI nr
Match: XP_022969472.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 478/575 (83.13%), Postives = 533/575 (92.70%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KS+ L Q+EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RAS+ALAGCKELMDF++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           AN+TG+A+EKMKKALK SM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300
           P+WA PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           Y+EYV++ K LTHLMLIGDGPKKTII+GNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGLRTCFYTE+NNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574
           +IT QHAVDFTPGRF+ GDRWIK TG+PYVSG+T+
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

BLAST of Cucsat.G7295 vs. NCBI nr
Match: XP_022931680.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 479/575 (83.30%), Postives = 534/575 (92.87%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KSV L Q+EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RAS+ALA CKELMD+++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300
           P+W +PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           Y+EYV ++K LTHLMLIGDGPKKTIITGNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGLRTCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574
           +ITPQHAVDFTPGRF+ GDRWIK TGVPYVSG+T+
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572

BLAST of Cucsat.G7295 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 1172 bits (3031), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583
           ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583

BLAST of Cucsat.G7295 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 1117 bits (2889), Expect = 0.0
Identity = 550/583 (94.34%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQEC+ASLKATGNNSSDP+ELVQAGFKAAMKLIQAAANKSV L QLEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAIDELKYSMNKLGEFDI+KLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGS+VKPNVVVAKDGSGQF+TIQ A+DQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEY+ +KKT+THLMLIGDGPKKTIITGNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS K   DL PVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGS+KSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTGGA AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAAAAH 583

BLAST of Cucsat.G7295 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 1117 bits (2889), Expect = 0.0
Identity = 550/583 (94.34%), Postives = 566/583 (97.08%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQEC+ASLKATGNNSSDP+ELVQAGFKAAMKLIQAAANKSV L QLEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAIDELKYSMNKLGEFDI+KLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGS+VKPNVVVAKDGSGQF+TIQ A+DQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEY+ +KKT+THLMLIGDGPKKTIITGNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS K   DL PVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGS+KSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTGGA AAAH
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAAAAH 583

BLAST of Cucsat.G7295 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 478/575 (83.13%), Postives = 533/575 (92.70%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KS+ L Q+EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RAS+ALAGCKELMDF++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           AN+TG+A+EKMKKALK SM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300
           P+WA PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           Y+EYV++ K LTHLMLIGDGPKKTII+GNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGLRTCFYTE+NNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574
           +IT QHAVDFTPGRF+ GDRWIK TG+PYVSG+T+
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

BLAST of Cucsat.G7295 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 479/575 (83.30%), Postives = 534/575 (92.87%), Query Frame = 0

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TSDPATG K+HEISSSMKA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAIC+PTDYK+ECV SLK +G   SDPKELVQ GFKAAMKLIQAAA KSV L Q+EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RAS+ALA CKELMD+++DELK+S+ KLG FDISKLDEMLIDI+IWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSML+ELQIPGI+RR LL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRR-LLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300
           P+W +PGM+RLLA   +K KPN+VVAKDGSGQ+KTIQEAI++VPKRKNNAT+VIHIK GV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           Y+EYV ++K LTHLMLIGDGPKKTIITGNKNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420
           TAGP KHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FKP ADLVPV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGLRTCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 574
           +ITPQHAVDFTPGRF+ GDRWIK TGVPYVSG+T+
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572