Cucsat.G7114 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G7114
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	(1->3)-beta-glucan endohydrolase
Location	ctg1528: 1550302 .. 1555103 (-)
RNA-Seq Expression	Cucsat.G7114
Synteny	Cucsat.G7114

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCTGCATTTATTATGGATTTATTGTTGAAAAATTGTTTTAAATGGAAAAATGGTTGAAAGTATTTTCAAATATAAATATTTTTCAGTTTTACTATCTAATTTTTCTTTAAAATATTCTTTTATTATTTTATTATTTTCTTGAAAAAGAGTTAAGAGGCATTAGGCCAACATTTAAAACACACCCCTTGTAAGCTATATCTTGTTTAACCAAATTTTATATCAATCTAAACTTATTTATAAATATATTATGTATTGAACTACGTAAAACTTATAGTGAATGAATCAATTTTTGTGAAGTAAATCAATTTAAACCTTAGTTAGAATTTCTTTGAAGAATGGTAAATAATAAAACCCTAACCATCCATTTTATAAATGCAACATTGAAAGAAGTGTCTACTTATATGTAATAATTAGTATAAACATTTGGTCGACTAAGGCTTATTTCAAACCATAAGTGGACATGTTAAGCTTGCAAACTAAATGTTGAGTATCAAGAGAAAGAGAGTCTTGTTAAATTACCTCTAAGGGATGTTGTTTCCCCTACCACTCTTGCATTTTCTTCATTCTACAATTCTAGTGTTGTAAGATAGAAAGTAAAACCAGAATTGGGTTTCGCGGGAGAAAGGATAGAAAATCCACGAGTCTTGGAAGGTGGAACGAAAGGACGAGGGGTTAATGGAAGGTGAAAGAGGAATGAAGAATGAAGTGGGGAGGAAGGAAGGAGGAAATGGAAGAAAGAGAATAAAGAAGGTGGTTCTGAAGGGAGAAAATGGAGAAGGTTGAAGAGGAAGAATGAGAAAGAAGAAGGAGAAAGAAGAATGAGAAGAAGACGAGATAGGAGGTTTGCTATTTTGTATAAATAGTTCTCCTCCTTTTTCTATTTTTAAAATCCCTAATGTACATATACAAATATAATCGATTTTGAAATGGATGAAGAAAATTGATATAGAAGAGGGTAGAGTGAATTTTTTGTTCTTAAATTTTGTAGTCAAAATTCTTTTCTCAAACTCCTTCCGTCAATAGTCAAAACCCTATTCACCTGTCAGTTTCCAATTGAATTTTCCATTGAGGGGGGACAGAGCTACACGGAGGAAGTTAATGACGCCCAACTGAAGAAAAGAAAAATCGCCATAGATGAGAAGGACAAGGATATACCCTTCTCCGAATTGGGTTTCTAAAGCACATGGCGGAAGGGTTACACATTTCTCTCTCACCCTTTGCTCTGTTTCTCATGACGAGGTTTTTGTTTTACAACATTCTCATATGAATCCATTCACCACTCCCTCATGGCTTCTTCCTCAATCTTCTTCTCCTCCTTTTCTTTCGTTCTTGTTCTTCTCCTTGGGGTTCTCATTTCTCCAGGTTTCAACCGATTAGTTGGTAATTTTCCTTTAATTTCTCTAGAAGATTATGGTTTTGGTCTCAATTTTACTAGGGTTTTTAATTGTATCTTGTGGTGAAGTAGGATTGAAGTTGGGAAATATGGCGATGGCGATGACGATTGAAGATGAAGATTCTATGGCTCTTTTACCATTTGGAGGATCAGCATCTAGTGTTGGACGCTTCATCCGACTTCCTCAATGCGGTGGCCCTCGCCGGACAGGTACCTCTTTCCGATCCTTGGCTTTAGCTTAAACTCAGTTTGATTATGAACTATTCCTGTATTTGGGATTCATGGCAAATTTCTGTATATGAGTGGCCTGTAATGTTTTTTTTACCCTTAAATTAAAGCTATTTCCTCTCAATTTCTCGGGGTTTACATCAATCAATCAGTTGAATTCTTAACTAAATTTTAAATATAAAAACAGGTCTTTAATAACTTCTTTTTCAAATACGAAACTTGACCAAGAAAGAAATTTAGAAATAGAATAGATATTTATAATATGCTTAATTTCAATTGAAAAATGTAGACAACCAAAAGGTTATAAAGAGTCTAAATCTTTTGTGTTCTGTAACTTAGGATATTGAGGTCCACTCTTTGGTTTATGGTTGTGTGTTCTGACCCTCTCATGACTCACAAGTCACCAGTCTCTAGAGGCTTTGTCCAATCTTACCCAACCTTGGGAGACAAGGAAGATTCTTTTATTCAAGGCGTTGTTGGATAATCATTGAGTTTTATTATTTTTTTCTCTCACTTTATACCTTCTTAGGTTTTGAAAACTAATTTTCTTTAGATTTCAAAGATTAACCTAAAAATAGATAATAAAACAGAGAAGTACATAGATGGAGGTAGTGTTTATCTAAGAGCAGACACAGATGCTCCACTAAAGAAAACATAAAATGTCGAGCTAATGAGCACTCTTTGGAGATGAAACGTTTTAGCCCCTCTTAGTAACCATTTCGTTTCTATATTTATTTTTGAAAATTAAATCTATTTCCTCTACGCATTTTACACTGATTTGCATCTTTATTAAATGCAAGTTGAATTGTTAGCAAATTTTCAAAAAGAATAACAATTCATTGAAAGCTATTTTTTCTTTAGTTTTCAAATTTTGGTTTGGATTTTTAAACCTTGGCAGAGTGGAGATAAAAAAGAAGAAAGTTGGAGGTGAGAATAATGTTTTTAGGCGTCAGTTTCAATAACAAAATGATTTCCAAACGGAGTTTTAGGTTTCTTATTTCAGTTTTCTATATTTTATTTTATATATACACACATATATTTCTAGGCATGTCCAAAAAGTGTCCCAACCTGAAATTTAAAGTAAATTAATTAATTAATTAAAATAGGTCACGAAAATTTTGTGTGTTGGACACATTTAGTGCTAATATAGACCCTCCTAAAATAGAGTGTCCGTGCTTTCACATGTGTTTGGAAGCTTAATTTTTCAAAGACCAAACGATTAGTTTTTAAGCCATATGACTTAGGAGTGGATTTTGGAAGCATCTCCAAACATCTGATAGCCTCCCCACTCCTAAATCTTTTCTAAAACCAAAACAGGAAGATGTCCCTACACTTTCAGATAATATCAAAAGGACTTGGAGAACGCAAGTGTCGCATCTACATTCACACCTACAACTACAAGATTATCCAACGAGTTGATTGTTTCAAACGTTTCTCATACTCCAACCCATATTTTACCTCTCTCCAATTATTGTTGGCATCATGGTCTAATCTGCTAACTACCTGATTGTGTTTAGAGTTTATGACCATGCATCCTTGTCAAATATTTTGGTGATAAAATGAATTAGAATTGTTATCAATATCCTCTAAACTGGATTGAGATCCCTTTTCGAAAGACAGTTGAGTCCACTGTTCTAACAAACCCTCTTGAGTGTGTGCCTAGGCTCAAGGTGCAAGTCTGGCTCCATGCCTTGAATAGGTGACGCTCACAAATTTAGATGGTGAATCCCAAGGGTCAAAGCCCTGAGTCTTGGGGCATTTCCTTAATTTTAAAATAACAGTAACAATTAGGAGTTTTAGCTTCTTTAGGAATGTTTTATTTCATATTTCCATTGCAACGATGGTTTCCCTTTATATAGTGCTTTTACATATACTGCGCCTCCAAAAATAAAAAAAATATATACGCACAATTTTTTTTCCACCTTGGCTCAAGCCCCAAAAGGTTATTGCGCCCGGTTTATTGTGCCTTTAGCTTTTAAAAACATTGGTTGAGCCCCCCCATCTTTTGTTCTGATCTCAAGTCCCTGAACTTATCCATCCGATTTGGGCTGGCTAAACATTTTATGCTGCATAAATCTTTGTTTTCTGCCTTGAAACTTCAACGTTACTTTTGTTTGAGCACTCATACGGTTGCACTTGCAGAATCCATTGGTGTTTGCTATTCAAGCGCAGGCAACAATTTACCAGATGCCAGAGAAGTAGTACAACTATACAAAAACAACAGCATAAAAAAGATGAGGATCTATAATCCCGACACAACTATATTAAACGCTCTTCGAGGATCGAACATTGAAGTCATTGTAGGCATTCCAAACACTTACATCGAACACATTGTTAATCTTTCTTCTGCAGCAAACTGGGTCCAAAAGAACATTCAAGCCTATGTGCCATATGTCAATTTCCGATACATTGCAGTTGGAAATGAGGTGAAGCCACCCGATGCCATTGCGCAATATGTTTTACCAGCCATGAGTAGCATTTATAGTGCAATTTCAGCAGCCAATTTACAAGATCAAATAAAGGTTTCCACAGTAATATCCATGAGCTTCTTGTCGAACTCCTCCTTTCCTCCATCTAATGGCTCATTTAGTTCAGAGGCAGGTGGATTTATGGAACCAATTATCAAGTTTCTGGCTAAGAATGGCTCACCACTTCTTGCTAATGTGTATCCCTACTTTGTTTACACTGAGATCAATCAAACCATCAGCCTTGACTATGCTTTGTTCATGCAATCGCCTGCTCTTGTGATTAGTGATGGAAATTTTAAGTACAACAGTCTTTTTGAATCAATGGTTGATGCTCTTTATGTAGCACTTGAGAAGTTGGGTGGAGCCAAAGTGTCGATTGTGATATCGGAGAGCGGTTGGCCATCAGGTGGAGGCAACGGTGCAACAATTGAGAATGCAGGTACGTACTATAGAAATTTGATTAGCTTTGTTCGGAATGGGACGCCGAGGAGGCCGAGAAGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAGAACTTGAAGTCTCTTGAAATGGAGAAACATTTTGGTTTATTCACACCTGATAAGAAATCAAAATACCAACTTAGTTTTAGTTAAGTAAAGGGTGTACGAATATACATAGCTTATAATTAAAATAAATCAATATATATATATATTCATTCACTTCTCATTTTAAATATAAAATAA

Coding sequence (CDS)

ATGGCTTCTTCCTCAATCTTCTTCTCCTCCTTTTCTTTCGTTCTTGTTCTTCTCCTTGGGGTTCTCATTTCTCCAGGTTTCAACCGATTAGTTGGATTGAAGTTGGGAAATATGGCGATGGCGATGACGATTGAAGATGAAGATTCTATGGCTCTTTTACCATTTGGAGGATCAGCATCTAGTGTTGGACGCTTCATCCGACTTCCTCAATGCGGTGGCCCTCGCCGGACAGAATCCATTGGTGTTTGCTATTCAAGCGCAGGCAACAATTTACCAGATGCCAGAGAAGTAGTACAACTATACAAAAACAACAGCATAAAAAAGATGAGGATCTATAATCCCGACACAACTATATTAAACGCTCTTCGAGGATCGAACATTGAAGTCATTGTAGGCATTCCAAACACTTACATCGAACACATTGTTAATCTTTCTTCTGCAGCAAACTGGGTCCAAAAGAACATTCAAGCCTATGTGCCATATGTCAATTTCCGATACATTGCAGTTGGAAATGAGGTGAAGCCACCCGATGCCATTGCGCAATATGTTTTACCAGCCATGAGTAGCATTTATAGTGCAATTTCAGCAGCCAATTTACAAGATCAAATAAAGGTTTCCACAGTAATATCCATGAGCTTCTTGTCGAACTCCTCCTTTCCTCCATCTAATGGCTCATTTAGTTCAGAGGCAGGTGGATTTATGGAACCAATTATCAAGTTTCTGGCTAAGAATGGCTCACCACTTCTTGCTAATGTGTATCCCTACTTTGTTTACACTGAGATCAATCAAACCATCAGCCTTGACTATGCTTTGTTCATGCAATCGCCTGCTCTTGTGATTAGTGATGGAAATTTTAAGTACAACAGTCTTTTTGAATCAATGGTTGATGCTCTTTATGTAGCACTTGAGAAGTTGGGTGGAGCCAAAGTGTCGATTGTGATATCGGAGAGCGGTTGGCCATCAGGTGGAGGCAACGGTGCAACAATTGAGAATGCAGGTACGTACTATAGAAATTTGATTAGCTTTGTTCGGAATGGGACGCCGAGGAGGCCGAGAAGAGCTATTGAAACGTATTTGTTTGCTATGTTTGATGAGAACTTGAAGTCTCTTGAAATGGAGAAACATTTTGGTTTATTCACACCTGATAAGAAATCAAAATACCAACTTAGTTTTAGTTAA

Protein sequence

MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQCGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS

Homology

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BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 360.5 bits (924), Expect = 2.4e-98
Identity = 189/320 (59.06%), Postives = 241/320 (75.31%), Query Frame = 0


Query: 78  ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTY 137
           +SIGVCY   GNNLP A +V+ LYK+N I  MRIY+P++  L ALRGS+IE+I+ +PNT 
Sbjct: 28  QSIGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDTLQALRGSDIELILDVPNTD 87

Query: 138 IEHIV-NLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDA---IAQYVLPAMSSIYSA 197
           ++ +  + S+AA WVQ N+  Y   V FRYIAVGNEV P  +    AQYVLPAM ++ SA
Sbjct: 88  LQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTGSNAQYAQYVLPAMKNVQSA 147

Query: 198 ISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVY 257
           I++A LQDQIKVST  + S +   S+PPS GSFS +   F+ PII FLA+NGSPLLAN+Y
Sbjct: 148 ITSAGLQDQIKVSTA-TFSAVLGKSYPPSEGSFSDDVSSFINPIISFLAENGSPLLANIY 207

Query: 258 PYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIV 317
           PYF YT   Q I LDYALF  S  +V+ DG+++Y +LF++++DALY ALEK GG+ + IV
Sbjct: 208 PYFSYTGDTQNIRLDYALFTAS-GVVVQDGSYQYQNLFDALLDALYAALEKAGGSNLKIV 267

Query: 318 ISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKS-LEM 377
           +SESGWPS GG  AT++NA TYY+NLI+ V+ GTPR+   AIETYLFAMFDEN K+ LE 
Sbjct: 268 VSESGWPSEGGTAATVDNARTYYKNLINHVKGGTPRK-SGAIETYLFAMFDENQKTGLET 327

Query: 378 EKHFGLFTPDKKSKYQLSFS 393
           EKHFGLFTP ++SKYQ+SFS
Sbjct: 328 EKHFGLFTPGQESKYQISFS 344

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BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 347.1 bits (889), Expect = 2.7e-94
Identity = 174/318 (54.72%), Postives = 236/318 (74.21%), Query Frame = 0


Query: 78  ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTY 137
           +S+GVCY   GNNLP A +VVQLYK+ +I++MR+Y+P+   L ALRGSNIEV++G+PN+ 
Sbjct: 31  QSVGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVMLGVPNSD 90

Query: 138 IEHI-VNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKP---PDAIAQYVLPAMSSIYSA 197
           +++I  N S+A NWVQ+N++ + P V FRYIAVGNEV P     ++ +Y+LPAM +I +A
Sbjct: 91  LQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRNIRNA 150

Query: 198 ISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVY 257
           IS+A LQ+ IKVS+ + M+ + N SFPPS GSF ++   F++PII F+ +  SPLL N+Y
Sbjct: 151 ISSAGLQNNIKVSSSVDMTLIGN-SFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLVNIY 210

Query: 258 PYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIV 317
           PYF Y    + ISL YALF  +P +V+ DG+  Y +LF++M DA+Y AL + GG  + IV
Sbjct: 211 PYFSYAGNPRDISLPYALF-TAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGSIEIV 270

Query: 318 ISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEME 377
           +SESGWPS G   AT  NA TYY+NLI  V+ G+PRRP + IETYLFAMFDEN K+ E+E
Sbjct: 271 VSESGWPSAGAFAATTNNAATYYKNLIQHVKRGSPRRPNKVIETYLFAMFDENNKNPELE 330

Query: 378 KHFGLFTPDKKSKYQLSF 392
           KHFGLF+P+K+ KY LSF
Sbjct: 331 KHFGLFSPNKQPKYPLSF 346

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BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot
Match: P52407 (Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis OX=3981 GN=HGN1 PE=1 SV=2)

HSP 1 Score: 339.0 bits (868), Expect = 7.3e-92
Identity = 172/317 (54.26%), Postives = 228/317 (71.92%), Query Frame = 0


Query: 80  IGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTYIE 139
           +GVCY   GNNLP   EV+ LYK ++I +MRIY+P+  +L ALRGSNIE+I+G+PN+ ++
Sbjct: 38  VGVCYGMQGNNLPPVSEVIALYKKSNITRMRIYDPNRAVLEALRGSNIELILGVPNSDLQ 97

Query: 140 HIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDA----IAQYVLPAMSSIYSAIS 199
            + N S+A +WVQKN++ +   V FRYIAVGNE+ P +     +AQ+VLPAM +I+ AI 
Sbjct: 98  SLTNPSNAKSWVQKNVRGFWSSVLFRYIAVGNEISPVNRGTAWLAQFVLPAMRNIHDAIR 157

Query: 200 AANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPY 259
           +A LQDQIKVST I ++ + N S+PPS G+F  +   +++PII FL+   SPLLAN+YPY
Sbjct: 158 SAGLQDQIKVSTAIDLTLVGN-SYPPSAGAFRDDVRSYLDPIIGFLSSIRSPLLANIYPY 217

Query: 260 FVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVIS 319
           F Y    + ISL YALF  SP++V+ DG   Y +LF++ +DALY ALE+  G  + +V+S
Sbjct: 218 FTYAYNPRDISLPYALF-TSPSVVVWDGQRGYKNLFDATLDALYSALERASGGSLEVVVS 277

Query: 320 ESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKH 379
           ESGWPS G   AT +N  TY  NLI  V+ GTP+RP RAIETYLFAMFDEN K  E+EKH
Sbjct: 278 ESGWPSAGAFAATFDNGRTYLSNLIQHVKGGTPKRPNRAIETYLFAMFDENKKQPEVEKH 337

Query: 380 FGLFTPDKKSKYQLSFS 393
           FGLF P+K  KY L+FS
Sbjct: 338 FGLFFPNKWQKYNLNFS 352

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BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 337.8 bits (865), Expect = 1.6e-91
Identity = 172/320 (53.75%), Postives = 236/320 (73.75%), Query Frame = 0


Query: 74  PRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGI 133
           P R +SIGVCY   GNNLP   EVV LYK+N+I +MR+Y+P+   L ALR SNI+V++ +
Sbjct: 23  PTRVQSIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDV 82

Query: 134 PNTYIEHIV-NLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYS 193
           P + ++ +  N S+A +W+++N+ AY P V+FRYIAVGNE+ P   +AQY+LPAM +IY+
Sbjct: 83  PRSDVQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYN 142

Query: 194 AISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANV 253
           A+S+A LQ+QIKVST +    L  +S+PPS G+FSS A  ++ PI++FLA NG+PLL NV
Sbjct: 143 ALSSAGLQNQIKVSTAVDTGVL-GTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNV 202

Query: 254 YPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSI 313
           YPYF YT     ISL YALF  S  +V+ DG F Y +LF+++VDA++ ALE++GGA V++
Sbjct: 203 YPYFSYTGNPGQISLPYALFTAS-GVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAV 262

Query: 314 VISESGWPS-GGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLE 373
           V+SESGWPS GGG  A+  NA TY +NLI  V  GTPRRP + IE Y+F MF+EN K+  
Sbjct: 263 VVSESGWPSAGGGAEASTSNARTYNQNLIRHVGGGTPRRPGKEIEAYIFEMFNENQKAGG 322

Query: 374 MEKHFGLFTPDKKSKYQLSF 392
           +E++FGLF P+K+  YQ+SF
Sbjct: 323 IEQNFGLFYPNKQPVYQISF 340

[Close]

BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot
Match: Q8VZJ2 (Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3702 GN=At4g16260 PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.9e-90
Identity = 164/321 (51.09%), Postives = 229/321 (71.34%), Query Frame = 0


Query: 74  PRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGI 133
           P   ES+GVCY   GNNLP   + + L++ N+I+++R+Y+P+   LNALR + IEVI+G+
Sbjct: 18  PTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQAALNALRNTGIEVIIGV 77

Query: 134 PNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSA 193
           PNT +  + N SSA +W+Q N+  Y P V+F+YIAVGNEV P +     VLPAM ++Y A
Sbjct: 78  PNTDLRSLTNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSPSNG-GDVVLPAMRNVYDA 137

Query: 194 ISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVY 253
           +  ANLQD+IKVST I M+ + N SFPPS+G F  +   +++P+I FL    S LLAN+Y
Sbjct: 138 LRGANLQDRIKVSTAIDMTLIGN-SFPPSSGEFRGDVRWYIDPVIGFLTSTNSALLANIY 197

Query: 254 PYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIV 313
           PYF Y +  + ISL YALF  SP++V+ DG+  Y +LF++++D +Y A+E+ GG  + +V
Sbjct: 198 PYFSYVDNPRDISLSYALF-TSPSVVVWDGSRGYQNLFDALLDVVYSAVERSGGGSLPVV 257

Query: 314 ISESGWPSGGGNGATIENAGTYYRNLISFVR--NGTPRRPRRAIETYLFAMFDENLKSLE 373
           +SESGWPS GGN A+ +NA  +Y NL S VR   GTP+RP R +ETYLFAMFDEN KS E
Sbjct: 258 VSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRGVETYLFAMFDENQKSPE 317

Query: 374 MEKHFGLFTPDKKSKYQLSFS 393
           +EK+FGLF P+K+ K+ ++FS
Sbjct: 318 IEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of Cucsat.G7114 vs. NCBI nr
Match: XP_004139045.1 (glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical protein Csa_006933 [Cucumis sativus])

HSP 1 Score: 756 bits (1953), Expect = 3.64e-275
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0


Query: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS 60
           MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS
Sbjct: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS 60

Query: 61  SVGRFIRLPQCGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILN 120
           SVGRFIRLPQCGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILN
Sbjct: 61  SVGRFIRLPQCGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILN 120

Query: 121 ALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIA 180
           ALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIA
Sbjct: 121 ALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIA 180

Query: 181 QYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKF 240
           QYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKF
Sbjct: 181 QYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKF 240

Query: 241 LAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYV 300
           LAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYV
Sbjct: 241 LAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYV 300

Query: 301 ALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLF 360
           ALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLF
Sbjct: 301 ALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLF 360

Query: 361 AMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392
           AMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS
Sbjct: 361 AMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392

[Close]

BLAST of Cucsat.G7114 vs. NCBI nr
Match: XP_038880622.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 630 bits (1625), Expect = 2.44e-225
Identity = 333/392 (84.95%), Postives = 350/392 (89.29%), Query Frame = 0


Query: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS 60
           MASSSIFFS+F  V VLLLG  I P        K GNM MA  IEDEDSMALLPF GSAS
Sbjct: 1   MASSSIFFSTFCSVFVLLLGAFIPPES------KSGNMVMA--IEDEDSMALLPF-GSAS 60

Query: 61  SVGRFIRLPQCGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILN 120
             G FIRL QCGGPRRTESIGVCYSSAGNNLPDA+EVVQLYK+N IKKMRIYNPD  ILN
Sbjct: 61  GAGSFIRLSQCGGPRRTESIGVCYSSAGNNLPDAKEVVQLYKSNDIKKMRIYNPDAAILN 120

Query: 121 ALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIA 180
           ALRGSNIE+ VGIPNT IEHIVNLSSAANWV+KNI AYVP V FRY+A+GNEV+P D I+
Sbjct: 121 ALRGSNIELTVGIPNTDIEHIVNLSSAANWVEKNILAYVPLVKFRYVAIGNEVQPSDPIS 180

Query: 181 QYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKF 240
           +YVLPAMSSI+SAISAANLQDQIK+STVIS SFLSNSSFPPSNGSFSSEAGGFMEPI+ F
Sbjct: 181 RYVLPAMSSIHSAISAANLQDQIKISTVISTSFLSNSSFPPSNGSFSSEAGGFMEPIVDF 240

Query: 241 LAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYV 300
           L KNGSPLLANVYPYFVYTEI +TISLDYALFMQSP+LVI DGNF YNSLFESMVDALYV
Sbjct: 241 LVKNGSPLLANVYPYFVYTEITRTISLDYALFMQSPSLVIRDGNFFYNSLFESMVDALYV 300

Query: 301 ALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLF 360
           ALEKLGG KVSIVISESGWPSGGG GAT+ENAGTYYRNLISFVR GTPRRPRRAIETYLF
Sbjct: 301 ALEKLGGDKVSIVISESGWPSGGGKGATVENAGTYYRNLISFVRTGTPRRPRRAIETYLF 360

Query: 361 AMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392
           AMFDENLKSLEMEKHFGLFTPDKKSK+QLSFS
Sbjct: 361 AMFDENLKSLEMEKHFGLFTPDKKSKFQLSFS 383

[Close]

BLAST of Cucsat.G7114 vs. NCBI nr
Match: XP_022961205.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 588 bits (1516), Expect = 8.57e-209
Identity = 307/382 (80.37%), Postives = 337/382 (88.22%), Query Frame = 0


Query: 11  FSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQ 70
           F  V VLLL  LIS  F+R +GLK GNM MA  IED DSMALLPF GSAS +G F RLPQ
Sbjct: 2   FRSVFVLLLASLISLVFHRFIGLKSGNMVMA--IEDVDSMALLPF-GSASRIGSFFRLPQ 61

Query: 71  CGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVI 130
           CGGPRRTESIGVCY++ GNNLPDAREVVQLYK + I+KMRIYNPDT ILNALRGSNIEVI
Sbjct: 62  CGGPRRTESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVI 121

Query: 131 VGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSI 190
           VGIPNT +E IVNLSSA+NWVQ+NIQAYVP++ FRY+AVGN+V+P D+IA+YVLPA+SSI
Sbjct: 122 VGIPNTDLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSI 181

Query: 191 YSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLA 250
            SAISAANLQDQIKVSTVIS+S LSNSSFPPS  SFSSEA GF+EPI+ FLAKNGSPLLA
Sbjct: 182 NSAISAANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLA 241

Query: 251 NVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKV 310
           NVYPYF YT+I +TISLDYALFMQSP LVI D NF+YNSLFESMVDALYVALEK GGA+V
Sbjct: 242 NVYPYFTYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEV 301

Query: 311 SIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSL 370
           SIVI+ESGWPS G   AT+ENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +
Sbjct: 302 SIVIAESGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPV 361

Query: 371 EMEKHFGLFTPDKKSKYQLSFS 392
           EMEKHFGLFT DKKSKYQLSFS
Sbjct: 362 EMEKHFGLFTADKKSKYQLSFS 380

[Close]

BLAST of Cucsat.G7114 vs. NCBI nr
Match: KAG6590190.1 (Lichenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577 bits (1486), Expect = 1.76e-204
Identity = 296/361 (81.99%), Postives = 325/361 (90.03%), Query Frame = 0


Query: 32  GLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQCGGPRRTESIGVCYSSAGNNL 91
           GLK GNM MA  IED DSMALLPF GSAS +G F RLPQCGGPRRTESIGVCY++ GNNL
Sbjct: 7   GLKSGNMVMA--IEDVDSMALLPF-GSASRIGSFFRLPQCGGPRRTESIGVCYTNLGNNL 66

Query: 92  PDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTYIEHIVNLSSAANWV 151
           PDAREVVQLYK + I+KMRIYNPDT ILNALRG NIEVIVGIPNT +E IVNLSSA+NWV
Sbjct: 67  PDAREVVQLYKIHGIEKMRIYNPDTAILNALRGLNIEVIVGIPNTDLERIVNLSSASNWV 126

Query: 152 QKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSAISAANLQDQIKVSTVISM 211
           Q+NIQAYVP++ FRY+AVGN+V+P D+IA+YVLPA+SSI SAISAANLQDQIKVSTVIS+
Sbjct: 127 QRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAANLQDQIKVSTVISI 186

Query: 212 SFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPYFVYTEINQTISLDYAL 271
           S LSNSSFPPS  SFSSEA GF+EPI+ FLAKNGSPLLANVYPYF YT+I++TISLDYAL
Sbjct: 187 SLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFTYTDISRTISLDYAL 246

Query: 272 FMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVISESGWPSGGGNGATIEN 331
           FMQSP LVI DGNF+YNSLFESMVDALYVALEK GGA+VSIVI+ESGWPS G   AT+EN
Sbjct: 247 FMQSPTLVIRDGNFEYNSLFESMVDALYVALEKSGGAEVSIVIAESGWPSNGSAAATVEN 306

Query: 332 AGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKHFGLFTPDKKSKYQLSF 391
           AGTYYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHFGLFT DKKSKYQLSF
Sbjct: 307 AGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFGLFTADKKSKYQLSF 364

[Close]

BLAST of Cucsat.G7114 vs. NCBI nr
Match: XP_022154372.1 (glucan endo-1,3-beta-glucosidase-like [Momordica charantia])

HSP 1 Score: 574 bits (1479), Expect = 5.28e-203
Identity = 304/394 (77.16%), Postives = 338/394 (85.79%), Query Frame = 0


Query: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS 60
           MASS   FS F  V +LLLG LIS GF R+ GLK GNMAMA+ IE+ DSMALLPF GSAS
Sbjct: 1   MASS--IFSKFCSVSLLLLGALISAGF-RIFGLKSGNMAMALAIEEVDSMALLPF-GSAS 60

Query: 61  SVGRFIRLPQCGGPRRT--ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTI 120
            +  F+RLPQCGGPR T  ES+GVCY+S+G+NLP A EVVQLYKNNSI KMRIYNP   I
Sbjct: 61  GIDSFVRLPQCGGPRPTVAESVGVCYASSGSNLPSATEVVQLYKNNSIGKMRIYNPSEAI 120

Query: 121 LNALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDA 180
           LNAL+GSNIEV+VGIPNT +EH+VNLSSAANWVQ+NIQAY+ +V FRYIA+GN+V P D+
Sbjct: 121 LNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQRNIQAYISHVKFRYIAIGNDVHPSDS 180

Query: 181 IAQYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPII 240
            A YVLPA+ SIY AISAANLQDQIKVSTVIS+S LSNSSFPPS GSFSSEA GF+EPI+
Sbjct: 181 FAGYVLPAIRSIYGAISAANLQDQIKVSTVISISLLSNSSFPPSIGSFSSEARGFIEPIV 240

Query: 241 KFLAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDAL 300
            FLA+NGSPLLANVYPYF YT+I +TISLDYALFMQSPAL I DGNF YNSLFESMVDAL
Sbjct: 241 SFLAENGSPLLANVYPYFTYTDIARTISLDYALFMQSPALAIRDGNFMYNSLFESMVDAL 300

Query: 301 YVALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETY 360
           Y ALEK GG +VSIVISESGWPS  G  ATIENAGTYYRNLI+FV++GTPRRP RAIETY
Sbjct: 301 YAALEKSGGNEVSIVISESGWPSSEGTAATIENAGTYYRNLINFVQSGTPRRPGRAIETY 360

Query: 361 LFAMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392
           LF+MFDENLK  E+EKHFGLFTP+KK KYQLSFS
Sbjct: 361 LFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390

[Close]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL
Match: A0A6J1HBJ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 588 bits (1516), Expect = 4.15e-209
Identity = 307/382 (80.37%), Postives = 337/382 (88.22%), Query Frame = 0


Query: 11  FSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQ 70
           F  V VLLL  LIS  F+R +GLK GNM MA  IED DSMALLPF GSAS +G F RLPQ
Sbjct: 2   FRSVFVLLLASLISLVFHRFIGLKSGNMVMA--IEDVDSMALLPF-GSASRIGSFFRLPQ 61

Query: 71  CGGPRRTESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVI 130
           CGGPRRTESIGVCY++ GNNLPDAREVVQLYK + I+KMRIYNPDT ILNALRGSNIEVI
Sbjct: 62  CGGPRRTESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVI 121

Query: 131 VGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSI 190
           VGIPNT +E IVNLSSA+NWVQ+NIQAYVP++ FRY+AVGN+V+P D+IA+YVLPA+SSI
Sbjct: 122 VGIPNTDLERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSI 181

Query: 191 YSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLA 250
            SAISAANLQDQIKVSTVIS+S LSNSSFPPS  SFSSEA GF+EPI+ FLAKNGSPLLA
Sbjct: 182 NSAISAANLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLA 241

Query: 251 NVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKV 310
           NVYPYF YT+I +TISLDYALFMQSP LVI D NF+YNSLFESMVDALYVALEK GGA+V
Sbjct: 242 NVYPYFTYTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEV 301

Query: 311 SIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSL 370
           SIVI+ESGWPS G   AT+ENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +
Sbjct: 302 SIVIAESGWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPV 361

Query: 371 EMEKHFGLFTPDKKSKYQLSFS 392
           EMEKHFGLFT DKKSKYQLSFS
Sbjct: 362 EMEKHFGLFTADKKSKYQLSFS 380

[Close]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL
Match: A0A6J1DLI3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 PE=3 SV=1)

HSP 1 Score: 574 bits (1479), Expect = 2.56e-203
Identity = 304/394 (77.16%), Postives = 338/394 (85.79%), Query Frame = 0


Query: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSAS 60
           MASS   FS F  V +LLLG LIS GF R+ GLK GNMAMA+ IE+ DSMALLPF GSAS
Sbjct: 1   MASS--IFSKFCSVSLLLLGALISAGF-RIFGLKSGNMAMALAIEEVDSMALLPF-GSAS 60

Query: 61  SVGRFIRLPQCGGPRRT--ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTI 120
            +  F+RLPQCGGPR T  ES+GVCY+S+G+NLP A EVVQLYKNNSI KMRIYNP   I
Sbjct: 61  GIDSFVRLPQCGGPRPTVAESVGVCYASSGSNLPSATEVVQLYKNNSIGKMRIYNPSEAI 120

Query: 121 LNALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDA 180
           LNAL+GSNIEV+VGIPNT +EH+VNLSSAANWVQ+NIQAY+ +V FRYIA+GN+V P D+
Sbjct: 121 LNALQGSNIEVVVGIPNTDLEHVVNLSSAANWVQRNIQAYISHVKFRYIAIGNDVHPSDS 180

Query: 181 IAQYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPII 240
            A YVLPA+ SIY AISAANLQDQIKVSTVIS+S LSNSSFPPS GSFSSEA GF+EPI+
Sbjct: 181 FAGYVLPAIRSIYGAISAANLQDQIKVSTVISISLLSNSSFPPSIGSFSSEARGFIEPIV 240

Query: 241 KFLAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDAL 300
            FLA+NGSPLLANVYPYF YT+I +TISLDYALFMQSPAL I DGNF YNSLFESMVDAL
Sbjct: 241 SFLAENGSPLLANVYPYFTYTDIARTISLDYALFMQSPALAIRDGNFMYNSLFESMVDAL 300

Query: 301 YVALEKLGGAKVSIVISESGWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETY 360
           Y ALEK GG +VSIVISESGWPS  G  ATIENAGTYYRNLI+FV++GTPRRP RAIETY
Sbjct: 301 YAALEKSGGNEVSIVISESGWPSSEGTAATIENAGTYYRNLINFVQSGTPRRPGRAIETY 360

Query: 361 LFAMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392
           LF+MFDENLK  E+EKHFGLFTP+KK KYQLSFS
Sbjct: 361 LFSMFDENLKPPEIEKHFGLFTPNKKPKYQLSFS 390

[Close]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL
Match: A0A6J1H9Q0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 PE=3 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 2.20e-180
Identity = 260/315 (82.54%), Postives = 287/315 (91.11%), Query Frame = 0


Query: 78  ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVIVGIPNTY 137
           ESIGVCY++ GNNLPDAREVVQLYK + I+KMRIYNPDT ILNALRGSNIEVIVGIPNT 
Sbjct: 6   ESIGVCYTNLGNNLPDAREVVQLYKIHGIEKMRIYNPDTAILNALRGSNIEVIVGIPNTD 65

Query: 138 IEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSIYSAISAA 197
           +E IVNLSSA+NWVQ+NIQAYVP++ FRY+AVGN+V+P D+IA+YVLPA+SSI SAISAA
Sbjct: 66  LERIVNLSSASNWVQRNIQAYVPHIKFRYVAVGNDVQPSDSIARYVLPAISSINSAISAA 125

Query: 198 NLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLANVYPYFV 257
           NLQDQIKVSTVIS+S LSNSSFPPS  SFSSEA GF+EPI+ FLAKNGSPLLANVYPYF 
Sbjct: 126 NLQDQIKVSTVISISLLSNSSFPPSYSSFSSEASGFIEPIVHFLAKNGSPLLANVYPYFT 185

Query: 258 YTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKVSIVISES 317
           YT+I +TISLDYALFMQSP LVI D NF+YNSLFESMVDALYVALEK GGA+VSIVI+ES
Sbjct: 186 YTDITRTISLDYALFMQSPTLVIRDDNFEYNSLFESMVDALYVALEKSGGAEVSIVIAES 245

Query: 318 GWPSGGGNGATIENAGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLKSLEMEKHFG 377
           GWPS G   AT+ENAGTYYRNL++FV  GTPRRPRRAIETYLFAMFDENLK +EMEKHFG
Sbjct: 246 GWPSNGSAAATVENAGTYYRNLMNFVPTGTPRRPRRAIETYLFAMFDENLKPVEMEKHFG 305

Query: 378 LFTPDKKSKYQLSFS 392
           LFT DKKSKYQLSFS
Sbjct: 306 LFTADKKSKYQLSFS 320

[Close]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL
Match: A0A6J1DNI9 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 PE=3 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 5.12e-164
Identity = 258/400 (64.50%), Postives = 309/400 (77.25%), Query Frame = 0


Query: 1   MASSSIFFSSFSFVLVLLLGVLISPGFNRLVGLKLGNMAMAMT--IEDEDSMALLPFGGS 60
           MASS   FS+F  + +LLLG LIS GF R++G K GNMAMAM   I++ DSMALLP  GS
Sbjct: 1   MASS--LFSTFCSLSLLLLGALISAGF-RIIGSKSGNMAMAMAMAIDEVDSMALLPL-GS 60

Query: 61  ASSVGRFIRLPQCGGPRRT----ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNP 120
            S +G FIRLPQCGGPR T    +++GVCYSS G+NLP+  EVVQLYK+  IK+MRIY+ 
Sbjct: 61  VSGIGSFIRLPQCGGPRPTVETVQAVGVCYSSMGDNLPEPGEVVQLYKSYGIKRMRIYDT 120

Query: 121 DTTILNALRGSNIEVIVGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVK 180
           +  ILNALRGSNIEV+VGIPN  ++ +VNLSSA +WVQ+NIQAY+P+VNFRY+ +GNEV+
Sbjct: 121 NKAILNALRGSNIEVVVGIPNNDLQRVVNLSSAESWVQRNIQAYIPHVNFRYVTIGNEVR 180

Query: 181 PPDAIAQYVLPAMSSIYSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFM 240
           P D+ A YVLPAM +IYSAISAANLQDQ+KVSTVIS   L  +S+PPS GSF S+A  F+
Sbjct: 181 PSDSFAGYVLPAMRNIYSAISAANLQDQMKVSTVISTVLL-GASYPPSAGSFRSDAREFI 240

Query: 241 EPIIKFLAKNGSPLLANVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESM 300
           EPI+ FLAKNGSPLLAN+YPY+ Y   N+ I+LDYAL        + DGN  Y++L ++ 
Sbjct: 241 EPIVSFLAKNGSPLLANLYPYYSYISNNRDITLDYALLYDYNFPTVVDGNLVYHNLLDAT 300

Query: 301 VDALYVALEKLGGAKVSIVISESGWPSGGGNGATIEN--AGTYYRNLISFVRNGTPRRPR 360
           VDALY ALEK GGA  SIVISESGWPSG G    +EN  AGTYYRNLI  V++G+PRRP 
Sbjct: 301 VDALYAALEKSGGANFSIVISESGWPSGAGTNTVVENEVAGTYYRNLIRHVQSGSPRRPG 360

Query: 361 RAIETYLFAMFDENLKSLEMEKHFGLFTPDKKSKYQLSFS 392
           RAIETYLF+MFDENL+  E EKHFGLFT D K KYQLS S
Sbjct: 361 RAIETYLFSMFDENLRPSETEKHFGLFTHDMKQKYQLSVS 395

[Close]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL
Match: A0A6J1DNH1 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 PE=3 SV=1)

HSP 1 Score: 457 bits (1176), Expect = 2.93e-157
Identity = 249/384 (64.84%), Postives = 300/384 (78.12%), Query Frame = 0


Query: 15  LVLLLGVLISPGFNRLVGLKLGNMAMAMTIEDEDSMALLPFGGSASSVGRFIRLPQCGGP 74
           L+LLLG  ISPG       K GN+A+A  IE+ DSMALLPF GSAS +G F+RLPQCGGP
Sbjct: 18  LLLLLGAFISPGS------KSGNVALA--IEEVDSMALLPF-GSASGIGSFVRLPQCGGP 77

Query: 75  RRT----ESIGVCYSSAGNNLPDAREVVQLYKNNSIKKMRIYNPDTTILNALRGSNIEVI 134
           R T    ++IGVCYS+ G+NLP+  EVV+LYK+  IK+MRIY+ D TILNALRGSNIEV+
Sbjct: 78  RPTVETVQAIGVCYSTKGDNLPEPGEVVRLYKSLGIKRMRIYDTDQTILNALRGSNIEVV 137

Query: 135 VGIPNTYIEHIVNLSSAANWVQKNIQAYVPYVNFRYIAVGNEVKPPDAIAQYVLPAMSSI 194
           VGIPNT +E + N+SSAANWVQ NIQA+VP+V FRYI VGNEV+P D+ A YVLPAM +I
Sbjct: 138 VGIPNTDLERVANVSSAANWVQMNIQAFVPHVKFRYITVGNEVQPSDSFAGYVLPAMRNI 197

Query: 195 YSAISAANLQDQIKVSTVISMSFLSNSSFPPSNGSFSSEAGGFMEPIIKFLAKNGSPLLA 254
           YSAISAANLQDQ+K+STVIS + L  +S PPS GSF S+A  F+EPI+ FLAK GSPLLA
Sbjct: 198 YSAISAANLQDQMKISTVISTALL-GTSHPPSAGSFGSDAREFIEPIVSFLAKKGSPLLA 257

Query: 255 NVYPYFVYTEINQTISLDYALFMQSPALVISDGNFKYNSLFESMVDALYVALEKLGGAKV 314
           N+Y Y+ Y  +++ ++LDYAL  +S   VI+DGN +Y++  ESM+DALY ALE  GGA V
Sbjct: 258 NLYTYYSYIGLSRQMNLDYALLKESQG-VIADGNLEYHNYVESMIDALYAALENSGGANV 317

Query: 315 SIVISESGWPSGGGNGATIEN--AGTYYRNLISFVRNGTPRRPRRAIETYLFAMFDENLK 374
           SIVISESGWPSG G    IE+  A TYY NLI  V++GTPRRP RAIETYLF++FDENL+
Sbjct: 318 SIVISESGWPSGAGTNTIIESVVAETYYVNLIRCVQSGTPRRPGRAIETYLFSIFDENLR 377

Query: 375 SLEMEKHFGLFTPDKKSKYQLSFS 392
             EMEKHFGLFT D + KYQ SFS
Sbjct: 378 PSEMEKHFGLFTHDMEPKYQFSFS 390

The following BLAST results are available for this feature:

BLAST of Cucsat.G7114 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PQW3	2.4e-98	59.06	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]
P07979	2.7e-94	54.72	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
P52407	7.3e-92	54.26	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform OS=Hevea brasiliensis O...	[more]
O22317	1.6e-91	53.75	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
Q8VZJ2	6.9e-90	51.09	Probable glucan endo-1,3-beta-glucosidase At4g16260 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cucsat.G7114 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139045.1	3.64e-275	100.00	glucan endo-1,3-beta-glucosidase [Cucumis sativus] >KAE8653516.1 hypothetical pr...	[more]
XP_038880622.1	2.44e-225	84.95	glucan endo-1,3-beta-glucosidase-like [Benincasa hispida]	[more]
XP_022961205.1	8.57e-209	80.37	glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata]	[more]
KAG6590190.1	1.76e-204	81.99	Lichenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022154372.1	5.28e-203	77.16	glucan endo-1,3-beta-glucosidase-like [Momordica charantia]	[more]

BLAST of Cucsat.G7114 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBJ9	4.15e-209	80.37	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P...	[more]
A0A6J1DLI3	2.56e-203	77.16	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021653 ...	[more]
A0A6J1H9Q0	2.20e-180	82.54	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461786 P...	[more]
A0A6J1DNI9	5.12e-164	64.50	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021658 ...	[more]
A0A6J1DNH1	2.93e-157	64.84	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111021643 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 79..393 e-value: 5.1E-126 score: 422.0
None	No IPR available	PANTHER	PTHR32227:SF267	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE	coord: 72..390
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 81..392 e-value: 4.3E-110 score: 368.2
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 311..324
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 72..390
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 81..392

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G7114.T1	Cucsat.G7114.T1	mRNA
Cucsat.G7114.T2	Cucsat.G7114.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006749	glutathione metabolic process
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds