Cucsat.G6734 (gene) Cucumber (B10) v3

Overview
NameCucsat.G6734
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionNEDD8-conjugating enzyme Ubc12-like
Locationctg1502: 906083 .. 911049 (-)
RNA-Seq ExpressionCucsat.G6734
SyntenyCucsat.G6734
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCAGTCGAAGCTCGAAAGCAAAGGGATCCCAATTTGGTCCTGCACAGAGCGTACGACGATAATTTTTCACAGAGAGAAAGAAAAGAGAGAGAGTTTTGATTCTGAATCAATCAACCCCATCTCCCTCTTTCTTTCTCTCTTCATTTCCGTCTTCATACTTTCAAGAATTTGTTTCTAATTTCTTCAAATCCACCCAAGTTCCGGTTCCATCCCTTCCCCTCCCCTTCTTTTTTTTTTTTTATGCTTCATTCACTGATCCAAGATTATTGATTTTGTGTTTGATGAATCTTATCTTGATTGCCCACTTGCATCTTATGTGTTCAACTCCCTTTTCCTGAGATCCTTATTGCTTCGGTTCTTATTTTTATTATTATTTTCATCTTTTTATGCATTTTTATGCTTCTTTTGATCCAACCAGTTAATTATTGATTGAATGATTATGTAAACGTATACACGATGTATTGGGGAACAGATGTGGTTGGGGAATATGAGGAGATTTCTCTAGACAAGTTTGGGGTCTCATTGGATTTTATTTTATTACAGCCAATTGTTGGTGGTATGGTGTAATATTCACGTTCTTTCTTTCTTTCTTTTTTTAATGTGTTTTCAATGGTAACAGGAGACGCGTTTTCACTATCCTGCTGTGTTATAACTTGATTGTTTTGTTTGGTAAAGTTGAGGTTAATTTTGTTAAGTCTTTACTGTTTTTGGTACTCAAGTTTACTGGTTTTTGAACTGGAAATAAAAGGAGGGAAAATAAACGTTTTTATCATCTTTAGTGGTGTAATTTGATTGGGATTAGTCATTAGTTCCTCAAAAGGTTTTTTTTGCAATGTTTGAACGTGGTCTGTTGCTAATTTGATATCTTCTTTTTACAGCAACCTGTAGAACGATGATTAAGCTTTTTAAAGTGAAGGAAAAGCAGAGAGAGATTGCAGCTAATGCCAATGGAGTACCTCCTGTCAAGAAGCAAAGTGCTGGAGAATTGCGTCTTCAAAAAGGTAGTTACTTATTTTCATCTTTGATGAAGAACTGTTGACCTTGAAGCCAACCGTGAAGCATTGAGTTTTGTCATTCTATGGTTTGTTGATTGTTGATGGGTAGTCATTCTTTTTCTAGAGCTGTTTATGAGAACCTATTTGGTTTGTTCTTTATATTAGCATTGTAGTCTCCATTGTACTCACTCATGTTCAGATCCGGTTTGTGTTATGAATTGTTTTTTTTTCCTTCCAAATCTGGTATTGCATGGCCATGAGGACATAGATCATCTTTTGGTACTGTGCCGGTTCCTCTTTTTCCTGGAACTTTATTGCTAAATCACTTATTAGTCTAGAATTAGCTTCTACCAGCCGAATGAGGTGAGAATATTACCAAGGCAGGGTCATTACCTTGTGACGTAATACAGTCAAAGTTTTGGACTCTAGTCTCTTACTAGCTTCCTCTTGGTTTATGTATTGAGGCCAACTGGAATTGCTTTTATAATCTCAGTCTGTGTGGCCTTGAGCGCAAGTTAGCTTTTTTTTTTCTTGTGTTTTCTTTTATTTGCTGAGTGATGTTTCTTATTCCAATTTCCAGTTATGTATCATAAATATAATATAGCATTAGTTTATTTTCTATTACTATGGTGTTATACAAGTGCTTGTGTATTTTGATATAATAGCAACTTTAAGTTTTGTTTTTTGTTATGTTTTGTTTTATTTGGTCTTCTGGCTGTACTTGGAGTTCAAGGAGTTTTTACTTTTAATTGTAGATATCAGTGAATTGAACCTACCAAAAACGTGTAATATATCATTTCCTAATGGCAAGGATGACTTGATGAACTTTGAGGTCACTATTCATCCTGATGAAGGATATTATCTGTAAGTAGTCTCTTGTAGATATTGTTTTTCAGTTTAGTTGTAACATATCTGCTTCTCAAGTGGAAATGCATGTGTAAGACATTACAGTTCCCGAATCTCAAACATACATGATGCATCTTATTATCATTTGAAAAACACTGGGAGCTGAAGATATATGCATGGATGCCATATCATTTCTATCAATGAGGTCTCAATGGAATTTTAGATTGGACTCAATTCATCTGTCTATAAACTGTGAATTTAAGCACTGCTTTTTTCATGATGTAATCATCATTTATTGGTAAAGATGTTTGTTCGTCCCAGCTGTTTCTAGGTTGTTAACTTAAGAATCCTTCGTTTTCTTGTTAACAGAGGCGGTACGTTTCTCTTCTCTTTCCAAGTTTCCCCTGTCTACCCTCACGAGCCACCAAAAGTGAAGTGCAAGACCAAGGTAACGATCCTGACCTTGTCGACTAATTTGTTTGTTGAAAATAGCTTAACACATTTGGCTTGCAGGTGTATCATCCTAATATTGACTTGGAAGGAAATGTTTGTCTCAATATTTTAAGAGAGGACTGGAAGCCCGTTCTGAATATCAACACTATAATATACGGATTGTTTCATCTTTTCACGGTATTTAATGATTGTACCATTGTCTAGCTTACTACAAACATTTTCTCATACTCCAATGACTCATTTCAAAGACCTTTAACACAATGGAATGTTTGCATAAATTCTAGATAGAGTTGAAAGCTCAAAGTTACTAGTTTGTAGGGTTCTCTTAATTACAATATATATTTTCCATTCTTGCAGCAACCAAATCATGAAGACCCCCTGAACCACGATGCTGCAGCTGTTTTAAGGGATAACCCGAAGATGTTTGAATTCAACGTGAGAAGAGCCATGGCTGGGGGTTATGTGGGACAAACTTACTTCACACGTTGCATGTAAGATTCTACATGAATTGTAAAGCAACTGATACATTATTTGAAGCATGGGCTCTGCGAGGTCGTTGAACTTCTCTGGTATGTTAATGTCTAATGTGTTGAGGACTGTCTGATCACCGACGCAACACAAAGTCCTTTGTGATGGAGGTAGAACTTTGTTGTGTGTTTGAAGTATTGGTTTCAAATGGACGTTGGGACTGGTAACTGAAACTCTTATCTGTGTATGTATTTTCCTCTAATTTATCTTGAAAAGACAGGTCGCTCTTTTGCTATCAGTTGAAAGTACAAGGTATGTTTTTGGAGCCTCCCTTCTTTTCTTTTATCAGAGTCTATTACCTACTATGGATGTAAATTTACTGAATTGTTGGATCACAATGATTTATTTCCTTTTATATATTTCAACTTTGTAAAATTCTCTGTTAGTTTCACATTTTGTTATCATCACAAGATTGGAAAGTTCAATCATTTTTTTCTTTACTTGCTCAGACCCTTTATCTCAATGGCCATTCTGATTGTGTTGCTTCAATGATTGTGTTATTGAAAAGCTTTTTGGGCCAAGTGACAGAAACCCCAATGAGCAATTTAGTTAAGTGGTAGGTAACATGGCTATCAGATCCTAGAGAGAGAACATATTTTCTAATGGATATAATAATGAGAATTGAGAACAATTTAAAATATTTGCAATTTATATTTCCATGATAGTTTTTCAAGAATTCTAATAATTTTTCTGATAATAGTTTTTGTTTTCTGCTTTCTAAATGAGGGACGATATGTATAAAGTCAATTTCACTGTGATCAGTGGAAATTAATATTTGCAAATTCTCTATTTTGGCTACTTTGTTTCTCTCAACAATGATTCCACTCTATTAAAATAATACTCGAGAAGTATTTTCAATGGTACAATGTCTGTTGATATAGCATTATATGCCCAAATTTCAACATTGAACTGTGGTAAAAGTAGAAATGAAACTAACGTAAAACAGAGGAAGCATATAAACTAAGCTACCTAAATTGATTCATCCTTTCCCCCCAAAAAAACTATATAACAAAACCATAAAAGGCTAAATATACAAGAAAAAAATAACTATGCAATCTGCTTGACTTCCTATGAACTGCTATGGTGCTATCGCATGCACAATGAATATCTCTAAACTTAACATATAAATCAACAGAATCTTCTTTTCTGCCAGAAGTGAATTCTCTCACCTGGATTAAAAATGGCAGCAAAGTAGTAAAGATGAGTGTTTGTCACCCTTCCTTCATTGACCGACTTCTCGACCCTTTCATCTCCCATGGTGGCAGGCAACCTTTATAATGCGAGTGAAGAAACAATGAAGAAATTGAAACTTCTCGTTGATGCATTGATATTGACGTGAAGCAGCCAAAACTACTTCGTAATTGCTTCTTCTCACTCTCGTATCCTTGAAAATTTGAGTCATTTGGAACACTCGAAGTCTTGAGGGCCTTGGGGAGCATGCAATTACATAAATATCCTACAACAAAAATGTAGGGAGTGATTTGGGGGCGTATTGATGATAACTATGAGAAGAGAATGAAAGCCTATGAATGCAACATATTATCTTGGAAGAAAATATAAATCTTATACATACAGTTTAGGAGTCTTTAGAAAGTAGAAAACTTGATGTTTATACATCTATTGGGTTACCTATTCTTGCAAGCCGATTCACCCATTTTGGTATGTGTTTCCCGGTTAACTTATAGCAAATATCCTCACAAAAATGGTTGCAATTTTTTACAATCAAGTGATACGTGTCACCATTGTAGTTTGCAGAATTGCGCTCCATGAACTCTCGAAATTGAATAGGATCCAAGCAAGTTGTACCCATGAAAATCGACTTTCTAAACTTAAACCCGGGGCAGCGTCGAGGTTCAACCTCGAAGACACCACTTGTTGGATAGTCATGGGCTCCGAAGGCATATTCCACACCATGAACTGTAAGTGAATTAAATGGAAATTAGAAATAACAATCATCGTCATGAAATATTCAGTTAAAAGAATGCATCACCCTGTCTAAATACTTCCTTGAATGAAGGTAAAGGCAGCTTGAGTCAGTAAAACGCAAAAGAGGTAGTTTTCCTACTATAAACTCGGTAATCTTGGACTCAATTTTTCATTTTGAAACAATGTTTTAAGACACTTTTATTTAAATAAGGAACAGATGATTATATCCACGAGAACAAAAGAAGACAACTTAAGCGCAAGGGTCAGGGAGACCTTCCCCCAGAAGAAACACAAGTGAATAAGGACCTTCCATTTGTTAAAAATAGAAAGATTATAATTACGAAAGTGTTTGACGTAGTTGTGTACGATGATGCTGAACCACAGTCCAAAAAGAATCAAAATTATCTTTAAAAATTCTACTATTTCTTTCGTTCCAAATATACAACAAAAGAGCTCTAGTTCTAGTGGTGCATTCTCCAAAGGATATTTCATTTCGGACCAGAAGCTTTACTTGGGAGGAAATAGTCCAAATAAAAGTTTCTAAACAAGAGTAACCACCCTGCCATTGCAAAATCAAAATGCAAAAAAAGGCAGAACCATTTTTGTGTATCTCAATTGCATTTTCCTTCCCCTTTTAGATAAGACATGTAAATGGCAAATAAATCTTTTGTTCTAGTGTCCATGGTCCTGTTTCCAAAACCCTTTTCATTCTTCCATACCTTGAATTTATTCGACAAGCTTATCGATGTTATATGGGTGATTATTTTCAATTTAACAGATACGAGCACAAAAAGAGATTGCAACTTCAAGATGCCTATTCTCCACAATGCATGCAGACGGAAATTACCATTTGTTCGTAGCAAATTTTAGTTAGCATCATCCTCACCCAAAAACAATGCATGCTACAAGAAACAAGCCAACAAACCTTGAATCCCAGAATGAAATATGCCAACACCTGCCCAGTAAACGCAGCCATTTACTGTTGTCAAGTCATACACATTGAGATATACTGGAGTGTTGCCCGGACCATATCTTGATCCCTTTGCTCTTGAAAAGATGCAAAAGTGAGAACCCGATTCGCTTCTCAAATGGAGAGGTATGATAGAGTTCCATCCCTTCTCTGATATATACTTCATGTTTTGGGGCGAAATGAGGAGAACATTCACATAGTTTCGACCTGCAGAACAATTACCAA

Coding sequence (CDS)

ATGATTAAGCTTTTTAAAGTGAAGGAAAAGCAGAGAGAGATTGCAGCTAATGCCAATGGAGTACCTCCTGTCAAGAAGCAAAGTGCTGGAGAATTGCGTCTTCAAAAAGATATCAGTGAATTGAACCTACCAAAAACGTGTAATATATCATTTCCTAATGGCAAGGATGACTTGATGAACTTTGAGGTCACTATTCATCCTGATGAAGGATATTATCTAGGCGGTACGTTTCTCTTCTCTTTCCAAGTTTCCCCTGTCTACCCTCACGAGCCACCAAAAGTGAAGTGCAAGACCAAGGTAACGATCCTGACCTTGTCGACTAATTTGTTTGTTGAAAATAGCTTAACACATTTGGCTTGCAGGTGTATCATCCTAATATTGACTTGGAAGGAAATGTTTGTCTCAATATTTTAA

Protein sequence

MIKLFKVKEKQREIAANANGVPPVKKQSAGELRLQKDISELNLPKTCNISFPNGKDDLMNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVTILTLSTNLFVENSLTHLACRCIILILTWKEMFVSIF
Homology
[Close]
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match: Q9SDY5 (NEDD8-conjugating enzyme Ubc12 OS=Arabidopsis thaliana OX=3702 GN=RCE1 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.3e-63
Identity = 108/125 (86.40%), Postives = 117/125 (93.60%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 60  MNFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 119

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPK+FE NVRRAM GGYVGQT+
Sbjct: 120 DWKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTF 179

Query: 121 FTRCM 126
           F RC+
Sbjct: 180 FPRCI 184

[Close]
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match: Q9ZU75 (Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana OX=3702 GN=RCE2 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.5e-62
Identity = 108/125 (86.40%), Postives = 115/125 (92.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTI PDEGYYL G F+FSFQVS +YPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 61  MNFEVTIKPDEGYYLSGNFVFSFQVSNMYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 120

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINT+IYGLFHLFT+PN+EDPLNH+AAAVLRDNPK FE+NVRRAM GG VGQT 
Sbjct: 121 DWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVRRAMMGGQVGQTS 180

Query: 121 FTRCM 126
           F RCM
Sbjct: 181 FPRCM 185

[Close]
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match: Q6DCZ9 (NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis OX=8355 GN=ube2m PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 6.4e-50
Identity = 86/125 (68.80%), Postives = 106/125 (84.80%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           +NF++ I PDEG+Y GG F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILRE
Sbjct: 58  LNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILRE 117

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVL IN+IIYGL +LF +PN EDPLN +AA VL++N ++FE NV+R+M GGY+G TY
Sbjct: 118 DWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTY 177

Query: 121 FTRCM 126
           F RC+
Sbjct: 178 FERCL 182

[Close]
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match: Q6P8D9 (NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropicalis OX=8364 GN=ube2m PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 6.4e-50
Identity = 86/125 (68.80%), Postives = 106/125 (84.80%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           +NF++ I PDEG+Y GG F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILRE
Sbjct: 58  LNFKLVICPDEGFYKGGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILRE 117

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVL IN+IIYGL +LF +PN EDPLN +AA VL++N ++FE NV+R+M GGY+G TY
Sbjct: 118 DWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTY 177

Query: 121 FTRCM 126
           F RC+
Sbjct: 178 FERCL 182

[Close]
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match: A3KN22 (NEDD8-conjugating enzyme Ubc12 OS=Bos taurus OX=9913 GN=UBE2M PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 3.2e-49
Identity = 85/125 (68.00%), Postives = 105/125 (84.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           +NF++ I PDEG+Y  G F+FSF+V   YPH+PPKVKC+T VYHPNIDLEGNVCLNILRE
Sbjct: 58  LNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILRE 117

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVL IN+IIYGL +LF +PN EDPLN +AA VL++N ++FE NV+R+M GGY+G TY
Sbjct: 118 DWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTY 177

Query: 121 FTRCM 126
           F RC+
Sbjct: 178 FERCL 182

[Close]
BLAST of Cucsat.G6734 vs. NCBI nr
Match: XP_004148299.1 (NEDD8-conjugating enzyme Ubc12 isoform X2 [Cucumis sativus] >XP_011659067.1 NEDD8-conjugating enzyme Ubc12 isoform X2 [Cucumis sativus] >XP_031744190.1 NEDD8-conjugating enzyme Ubc12 isoform X2 [Cucumis sativus] >KGN44318.1 hypothetical protein Csa_016775 [Cucumis sativus])

HSP 1 Score: 273 bits (698), Expect = 6.74e-92
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. NCBI nr
Match: XP_008447054.1 (PREDICTED: NEDD8-conjugating enzyme Ubc12-like [Cucumis melo])

HSP 1 Score: 273 bits (698), Expect = 6.74e-92
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. NCBI nr
Match: XP_031744189.1 (NEDD8-conjugating enzyme Ubc12 isoform X1 [Cucumis sativus])

HSP 1 Score: 273 bits (698), Expect = 2.45e-91
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 97  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 156

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 157 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 216

Query: 121 FTRCM 125
           FTRCM
Sbjct: 217 FTRCM 221

[Close]
BLAST of Cucsat.G6734 vs. NCBI nr
Match: KAA0036519.1 (NEDD8-conjugating enzyme Ubc12-like [Cucumis melo var. makuwa] >TYK13462.1 NEDD8-conjugating enzyme Ubc12-like [Cucumis melo var. makuwa])

HSP 1 Score: 273 bits (698), Expect = 5.14e-91
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 119 MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 178

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 179 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 238

Query: 121 FTRCM 125
           FTRCM
Sbjct: 239 FTRCM 243

[Close]
BLAST of Cucsat.G6734 vs. NCBI nr
Match: XP_038888942.1 (NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida] >XP_038888944.1 NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida])

HSP 1 Score: 270 bits (690), Expect = 1.12e-90
Identity = 124/125 (99.20%), Postives = 124/125 (99.20%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFE NVRRAMAGGYVGQTY
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGQTY 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match: A0A0A0K481 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253730 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 3.26e-92
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match: A0A1S3BHD5 (NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo OX=3656 GN=LOC103489610 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 3.26e-92
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match: A0A5D3CPZ9 (NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold179G00220 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 2.49e-91
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 119 MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 178

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY
Sbjct: 179 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 238

Query: 121 FTRCM 125
           FTRCM
Sbjct: 239 FTRCM 243

[Close]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match: A0A6J1C2V1 (NEDD8-conjugating enzyme Ubc12-like OS=Momordica charantia OX=3673 GN=LOC111007951 PE=3 SV=1)

HSP 1 Score: 267 bits (683), Expect = 6.31e-90
Identity = 122/125 (97.60%), Postives = 124/125 (99.20%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPK+FE NVRRAMAGGYVGQT+
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKLFESNVRRAMAGGYVGQTF 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match: A0A6J1JMG9 (NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita maxima OX=3661 GN=LOC111486523 PE=3 SV=1)

HSP 1 Score: 266 bits (681), Expect = 1.27e-89
Identity = 122/125 (97.60%), Postives = 123/125 (98.40%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 59  MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 118

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFE NVRRAMAGGYVG T+
Sbjct: 119 DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFESNVRRAMAGGYVGHTF 178

Query: 121 FTRCM 125
           FTRCM
Sbjct: 179 FTRCM 183

[Close]
BLAST of Cucsat.G6734 vs. TAIR 10
Match: AT4G36800.1 (RUB1 conjugating enzyme 1 )

HSP 1 Score: 243.4 bits (620), Expect = 9.5e-65
Identity = 108/125 (86.40%), Postives = 117/125 (93.60%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 60  MNFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 119

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPK+FE NVRRAM GGYVGQT+
Sbjct: 120 DWKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTF 179

Query: 121 FTRCM 126
           F RC+
Sbjct: 180 FPRCI 184

[Close]
BLAST of Cucsat.G6734 vs. TAIR 10
Match: AT4G36800.2 (RUB1 conjugating enzyme 1 )

HSP 1 Score: 243.4 bits (620), Expect = 9.5e-65
Identity = 108/125 (86.40%), Postives = 117/125 (93.60%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEV+I PD+GYY  GTF+F+FQVSPVYPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 60  MNFEVSIKPDDGYYHNGTFVFTFQVSPVYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 119

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINT+IYGLFHLFT+PN EDPLNHDAAAVLRDNPK+FE NVRRAM GGYVGQT+
Sbjct: 120 DWKPVLNINTVIYGLFHLFTEPNSEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTF 179

Query: 121 FTRCM 126
           F RC+
Sbjct: 180 FPRCI 184

[Close]
BLAST of Cucsat.G6734 vs. TAIR 10
Match: AT2G18600.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 240.0 bits (611), Expect = 1.0e-63
Identity = 108/125 (86.40%), Postives = 115/125 (92.00%), Query Frame = 0 

Query: 1   MNFEVTIHPDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILRE 60
           MNFEVTI PDEGYYL G F+FSFQVS +YPHE PKVKCKTKVYHPNIDLEGNVCLNILRE
Sbjct: 61  MNFEVTIKPDEGYYLSGNFVFSFQVSNMYPHEAPKVKCKTKVYHPNIDLEGNVCLNILRE 120

Query: 61  DWKPVLNINTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVRRAMAGGYVGQTY 120
           DWKPVLNINT+IYGLFHLFT+PN+EDPLNH+AAAVLRDNPK FE+NVRRAM GG VGQT 
Sbjct: 121 DWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVRRAMMGGQVGQTS 180

Query: 121 FTRCM 126
           F RCM
Sbjct: 181 FPRCM 185

[Close]
BLAST of Cucsat.G6734 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 100.9 bits (250), Expect = 7.6e-22
Identity = 44/100 (44.00%), Postives = 60/100 (60.00%), Query Frame = 0 

Query: 9   PDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNI 68
           P E  Y GG FL S    P YP +PPKV  KTKVYHPNI+  G++CL+IL+E W P L I
Sbjct: 40  PPESPYAGGVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTI 99

Query: 69  NTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVR 109
           + ++  +  L T PN +DPL  + A + + +   +E   R
Sbjct: 100 SKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTAR 139

[Close]
BLAST of Cucsat.G6734 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 100.9 bits (250), Expect = 7.6e-22
Identity = 44/100 (44.00%), Postives = 60/100 (60.00%), Query Frame = 0 

Query: 9   PDEGYYLGGTFLFSFQVSPVYPHEPPKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNI 68
           P E  Y GG FL S    P YP +PPKV  KTKVYHPNI+  G++CL+IL+E W P L I
Sbjct: 40  PPESPYAGGVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTI 99

Query: 69  NTIIYGLFHLFTQPNHEDPLNHDAAAVLRDNPKMFEFNVR 109
           + ++  +  L T PN +DPL  + A + + +   +E   R
Sbjct: 100 SKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTAR 139

The following BLAST results are available for this feature:
BLAST of Cucsat.G6734 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SDY51.3e-6386.40NEDD8-conjugating enzyme Ubc12 OS=Arabidopsis thaliana OX=3702 GN=RCE1 PE=1 SV=1[more]
Q9ZU751.5e-6286.40Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q6DCZ96.4e-5068.80NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis OX=8355 GN=ube2m PE=2 SV=1[more]
Q6P8D96.4e-5068.80NEDD8-conjugating enzyme Ubc12 OS=Xenopus tropicalis OX=8364 GN=ube2m PE=2 SV=1[more]
A3KN223.2e-4968.00NEDD8-conjugating enzyme Ubc12 OS=Bos taurus OX=9913 GN=UBE2M PE=2 SV=1[more]
BLAST of Cucsat.G6734 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004148299.16.74e-92100.00NEDD8-conjugating enzyme Ubc12 isoform X2 [Cucumis sativus] >XP_011659067.1 NEDD... [more]
XP_008447054.16.74e-92100.00PREDICTED: NEDD8-conjugating enzyme Ubc12-like [Cucumis melo][more]
XP_031744189.12.45e-91100.00NEDD8-conjugating enzyme Ubc12 isoform X1 [Cucumis sativus][more]
KAA0036519.15.14e-91100.00NEDD8-conjugating enzyme Ubc12-like [Cucumis melo var. makuwa] >TYK13462.1 NEDD8... [more]
XP_038888942.11.12e-9099.20NEDD8-conjugating enzyme Ubc12-like [Benincasa hispida] >XP_038888944.1 NEDD8-co... [more]
BLAST of Cucsat.G6734 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K4813.26e-92100.00UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G253730 PE... [more]
A0A1S3BHD53.26e-92100.00NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo OX=3656 GN=LOC103489610 PE=3... [more]
A0A5D3CPZ92.49e-91100.00NEDD8-conjugating enzyme Ubc12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1C2V16.31e-9097.60NEDD8-conjugating enzyme Ubc12-like OS=Momordica charantia OX=3673 GN=LOC1110079... [more]
A0A6J1JMG91.27e-8997.60NEDD8-conjugating enzyme Ubc12-like OS=Cucurbita maxima OX=3661 GN=LOC111486523 ... [more]
BLAST of Cucsat.G6734 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G36800.19.5e-6586.40RUB1 conjugating enzyme 1 [more]
AT4G36800.29.5e-6586.40RUB1 conjugating enzyme 1 [more]
AT2G18600.11.0e-6386.40Ubiquitin-conjugating enzyme family protein [more]
AT3G08690.17.6e-2244.00ubiquitin-conjugating enzyme 11 [more]
AT3G08690.27.6e-2244.00ubiquitin-conjugating enzyme 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 54..196
e-value: 4.2E-37
score: 139.3
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 38..195
NoneNo IPR availablePANTHERPTHR24068:SF379SUBFAMILY NOT NAMEDcoord: 38..195
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 55..190
e-value: 3.9E-38
score: 130.2
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 51..196
score: 37.739197
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 55..189
e-value: 1.98581E-40
score: 132.711
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 47..205
e-value: 1.4E-47
score: 163.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 42..202
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 123..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G6734.T9Cucsat.G6734.T9mRNA
Cucsat.G6734.T7Cucsat.G6734.T7mRNA
Cucsat.G6734.T6Cucsat.G6734.T6mRNA
Cucsat.G6734.T2Cucsat.G6734.T2mRNA
Cucsat.G6734.T20Cucsat.G6734.T20mRNA
Cucsat.G6734.T8Cucsat.G6734.T8mRNA
Cucsat.G6734.T5Cucsat.G6734.T5mRNA
Cucsat.G6734.T4Cucsat.G6734.T4mRNA
Cucsat.G6734.T1Cucsat.G6734.T1mRNA
Cucsat.G6734.T3Cucsat.G6734.T3mRNA
Cucsat.G6734.T11Cucsat.G6734.T11mRNA
Cucsat.G6734.T10Cucsat.G6734.T10mRNA
Cucsat.G6734.T12Cucsat.G6734.T12mRNA
Cucsat.G6734.T13Cucsat.G6734.T13mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045116 protein neddylation
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0019788 NEDD8 transferase activity