Cucsat.G6547 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G6547
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Splicing factor U2af small subunit B-like
Location	ctg1450: 607429 .. 610765 (+)
RNA-Seq Expression	Cucsat.G6547
Synteny	Cucsat.G6547

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATATAACTAAGTTGAATTAAATAGTAATGAATCTTCTAGACGTCCCCCCAAGGCTTTCCCATGAACATTCTAGAGAAACAGCTTAAATAAAACTCCACCCATCATCATTTTCTTTCTCTTCTTCTTCTGCTTCTTCTCCATTTTCATCTCATTCCAATTCTGTACCCAATTTCTTCCTTTCTCCCACGGCTGGAAATGGCGGAGCATTTGGCATCCATATTCGGAACCGAGAAGGACAGAGTTAACTGTCCCTTCTACTTCAAGATCGGGGCCTGCCGCCACGGTGATAGGTGTTCACGGTTGCACACCAAGCCTAGTATCAGCCCCACCCTCCTGCTTTCTAATATGTATCAGAGACCTGACATGATTACTCCTGGTGTCGACGCGCAAGGGAATCCAATTGACCCTCGCAACATCCAGGACCATTTTGAGGTACATTTTCTCTTGTTTACTACTATTTTTATCATGAATTTTCACGTTATTATTATTATTTTTGAACGGGGGTCGGGGTGGATCTTGGCTTTTTTTTTAGGTTGGATTGCTATTATTTTTATCGTAATCTTAGACGTCGTTTTGTTTAGTTGATTTTCAAGTGATCGTGTTATCATGGATAGAGTAAGATATCTTTTTTGTGTCCTCATTAACTTCCTTACGCTCTTGGCGTGGCTTGTAAATTTTTTTATTTTGAATCATCATTTGCGCGTGGAACAATTGTAGCTTTATTGTTGATCTGTCTACATTTCAGGAGTTCTATGAAGATCTATTTCAGGAACTGAACAAGTACGGGGAGATTGAAAGTTTGAATGTATGTGACAATCTAGCTGACCATATGGTAATACTCTCCTCTTTGCTCATTGTTTGAAGGGAAATAATTTTACAAATCTGCCTTTCAACTTTGATGTATGCTATTTCATTGTAGAATTAGCTAATGAGTAGTTATCTAATGATGTGTTTGATTAGGTGGGCAATGTTTATGTTCAATTTCGAGAGGAGGAGCAAGCAGCAAATGCTCTTCGGAATCTTAGTGGCAGATTCTATGCTGGTCAGTTGAAGTTTCTGTCTTCTCCTGCCCCTTTATTTTTCCCTTCATATTTATACTTTTTGTCTGCAGCTTTTGATTTTAGAATCGGGTGAAATGAATCTGGTAACCTATGTATATAACTGTTTCTTTGCAGGACGCCCGATCATTGTCGACTTCTCTCCAGTTACAGACTTTAGAGAAGCTACATGTAGGCAATATGAAGAAAACATGTGTAATCGTGGTGGGTATTGCAATTTCATGCATTTGAAGAGGATTGGCAGGTATATTATCTTCACCACCTCCCTCTTTTCCCTCTGAGATGTATTATTCTTCCTGTTGATATTGTATTGCCACTTCTTGTAAGTTGTATGCGTACTTTATTTGATAGCCATGGTAATTTCTTGTCTTTCAGAGAGCTGAGGCATGAACTGTTTGCAATGTATCGTCGAAGGCATAGTCATAGTAGGAGCAGAAGCCGCAGCCCCTATAGGCATCGAAGTTATGAAGAACGCTCTTATGGCAAGCATGGTCATAGTCGAAGGTATGATGAGAGAGATGCGTATCATGAAAGTCGGAGTAGGAGGCATAGGACTACAAGCCCTGGCCATAGAAGCAGGAGCCGTAGTCCACGTGGAAGGAAGAACCGAAGTCCAGTTAGGGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAAGGAGAGAGAACAAGGAAATGATAATAATGCCAATTCTGATGACAACAGAAATAACCATGAGAAAAGCTACGACAGTGAGGTGAAGTATGCAAATCAAACCTGTGGCTATGAGGAGCAGCAACAGCGGCAGCCTCCTGAGCAAGGCTATGGTTACTGATCTGTTTACTTGAACTTAGGTACTACTTCCAAGGTTTCTGTTTGAGGTCTTGCATTCATTCCTTAAAATGTCCCTAGTAGTGTTATCCCAATACGTAAGAAGTTGCTGGTTCTAGTTATCTGATCTCCCCAACATCTCTTTGATCTTTTTTTAGCCATTAGAGTAGGTAACAACATTTTTTTCATTAGTGGATTGGTTAGGAACGTGTCCAGCTTAGGGAACTTAAAAGAAAAAATAGAAAAATGGAAGGGGAAACTGTTTGCGAGACAGAACAAATGAAACAGTGCTAGAAGATGCAAGAAATGGTGGTTAAAAATTAAGTTCTTTTCCCCTATTGGATGTTAACCAGTTTATTCCCTTTGATTTGTGGAAAGTGAGAGAATGTGTAGAAGGAAACCCCCTGTCTTATCAGAAGATTCTTCAGTTAACCCTGTGGTACATCTAGAAAGCATCATAAAAATTGAAATAGATCTTTGAAAACCTCTTTTTCTTGACAGCGATTTGTTTTTGGACGTGTGTTTGTCTTCATTTAAATTTCATTAACATGTTTCAATTGCAGTCATGGCATTGATACTTACAATATCCATAATTCTATAGCAAACGTATAAATTTGATTTTACAGTCAGGGATTAAGACTGCTGCCCATGAGGACTTGTGAACGATTCCATTTATCGTATTACGGTTTCTTTCCCTTATGAACTTTTTTGCATTACCTTTTTGTTGATATTAAGAAAAAATGTGATCTCATCATTATCTAAGTTTTTGCAGGTTCAGGTTGTTTCCCTGCTTCTAGACTCTAAATTTTCCAAGCTAAGATGTGATCATGAAATTTTTGTACAGGTTAATATGGTCCACTGTGTAGGATGATTGGCTTTACAGGATGTGCAGGTTTTGGCCCTAACAAAGATGAGGCATTCGTGTGGATGGCTGAAGACTGAAGGCAACATTGAGTCACTTTGGCGTCTTTAACATTGATGTGGTGGGCTGCAATTGCAGGTTAGGGATCATGCTAGAAACATTTTGTATTTGTTTCATATATTGTTGTTAAAAGGAAGAGAACTTGGGGGTGGGGGTGTTTGTGATGGAGTAAATAGCCTTAAGATATATCTGATTGTGACTTTCTTTAAAAACTGGACTCACTAGTGATTTCTTATTGAGTGGTATAGAAAGCTCTTTCTCTATATTATATGATTAGATTAGATGTTAGAATATATAGCATTTACATGTGATTTAACTGTTTTGGGATTTAATAGGTTAGGGGATAAGTTTAGATTTATGATATACAATATAATTTTTGGATCTTTCATCAAACACGTTCCAATTTTGGATATTTTTGCGTGCAAGGACAGTAGAATTTTGTAAGGTTAGGGAACTCATATTTCATTCAAATTTTACAAACTTCTTACATTTTATGGTGCCACAAATATTCCGTTATTTAATTTTCATATTTTATT

Coding sequence (CDS)

ATGGCGGAGCATTTGGCATCCATATTCGGAACCGAGAAGGACAGAGTTAACTGTCCCTTCTACTTCAAGATCGGGGCCTGCCGCCACGGTGATAGGTGTTCACGGTTGCACACCAAGCCTAGTATCAGCCCCACCCTCCTGCTTTCTAATATGTATCAGAGACCTGACATGATTACTCCTGGTGTCGACGCGCAAGGGAATCCAATTGACCCTCGCAACATCCAGGACCATTTTGAGGAGTTCTATGAAGATCTATTTCAGGAACTGAACAAGTACGGGGAGATTGAAAGTTTGAATGTATGTGACAATCTAGCTGACCATATGGTGGGCAATGTTTATGTTCAATTTCGAGAGGAGGAGCAAGCAGCAAATGCTCTTCGGAATCTTAGTGGCAGATTCTATGCTGGACGCCCGATCATTGTCGACTTCTCTCCAGTTACAGACTTTAGAGAAGCTACATGTAGGCAATATGAAGAAAACATGTGTAATCGTGGTGGGTATTGCAATTTCATGCATTTGAAGAGGATTGGCAGAGAGCTGAGGCATGAACTGTTTGCAATGTATCGTCGAAGGCATAGTCATAGTAGGAGCAGAAGCCGCAGCCCCTATAGGCATCGAAGTTATGAAGAACGCTCTTATGGCAAGCATGGTCATAGTCGAAGGTATGATGAGAGAGATGCGTATCATGAAAGTCGGAGTAGGAGGCATAGGACTACAAGCCCTGGCCATAGAAGCAGGAGCCGTAGTCCACGTGGAAGGAAGAACCGAAGTCCAGTTAGGGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAAGGAGAGAGAACAAGGAAATGATAATAATGCCAATTCTGATGACAACAGAAATAACCATGAGAAAAGCTACGACAGTGAGGTGAAGTATGCAAATCAAACCTGTGGCTATGAGGAGCAGCAACAGCGGCAGCCTCCTGAGCAAGGCTATGGTTACTGA

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGRELRHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDSEVKYANQTCGYEEQQQRQPPEQGYGY

Homology

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BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 6.7e-107
Identity = 205/294 (69.73%), Postives = 230/294 (78.23%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH KPS+SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           G+DAQGNPIDP  IQ  FE+FYED+F+EL+KYGEIESL+VCDN ADHM+GNVYVQFREE+
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAA AL+ L+GR+Y+GRPIIV+FSPV+DFREATCRQYEEN CNRGGYCNFMH+K IGR+L
Sbjct: 121 QAARALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDL 180

Query: 181 RHELFA---MYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYH-------- 240
           R  LF      RR HSH RSRS SPY +R    R Y +   SR  D  D Y         
Sbjct: 181 RKRLFGHLHRSRRSHSHGRSRSPSPYHYR----RDYDRRSSSRSRDHDDYYRGGSHDYYR 240

Query: 241 ---ESRSRRHRTT--SPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKERE 279
                 S RHR++  S G R R RS    + RSPVR+GSEERRA+IEQWN+ERE
Sbjct: 241 GGSRRSSERHRSSYDSDGSRRRHRS----RTRSPVRDGSEERRAQIEQWNRERE 286

[Close]

BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.5e-106
Identity = 205/293 (69.97%), Postives = 231/293 (78.84%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP  IQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
            AA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+I REL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RHELFAMYR---RRHSHSRSRSRSPYRHRSY-EERSYG------KHGHSRRYDERDAYHE 240
           R +LF  YR   RR S SRSRS SP R R +  ER  G      +HG+ +R  +R   H+
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 ---SRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG 281
                 RRH     G   RSRSPR       VREGSEERRA+IEQWN+ER++G
Sbjct: 241 RDGGGRRRH-----GSPKRSRSPRN------VREGSEERRARIEQWNRERDEG 282

[Close]

BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 6.9e-104
Identity = 198/298 (66.44%), Postives = 227/298 (76.17%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPR IQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K + REL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RHELFAMYRRRH-----SHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRR 240
           R +LF  YRR +     S SRSRS SP   R  + R       S R  +R+ Y     + 
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGK- 240

Query: 241 HRTTSPGHRSRSRSPRGRKNRSPV--------REGSEERRAKIEQWNKEREQGNDNNA 286
            R++    R      RGR+  SP         REGSEERRA+IEQWN+ERE+  +  A
Sbjct: 241 -RSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREEKEEGGA 296

[Close]

BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.1e-99
Identity = 193/304 (63.49%), Postives = 224/304 (73.68%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P++SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG PIDP  +Q+HFE+FYED+++EL+K+GE+E+LNVCDNLADHM+GNVYVQFREEE
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QA  A   L GRFY+GRPIIV++SPVTDFREATCRQ+EEN CNRGGYCNFMH+K+IGREL
Sbjct: 121 QAVAAHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEE------------------RSYGKHGHSRRY 240
           R +L+   R R SH RSRS SP   R   +                  R  G  G    Y
Sbjct: 181 RRKLYG-GRSRRSHGRSRSPSPRHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGDGY 240

Query: 241 DERDAY-------HESRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNK 280
              D+Y             R+     G R R  SP  R+ RSPVRE SEERRAKIEQWN+
Sbjct: 241 RGGDSYRGGGGGGRRGGGSRYDRYDDGGRRRHGSP-PRRARSPVRESSEERRAKIEQWNR 300

[Close]

BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot
Match: A1A4K8 (Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-65
Identity = 130/235 (55.32%), Postives = 162/235 (68.94%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSRLH KP+ S T+ L N+Y+ P   + 
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQ 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQEL-NKYGEIESLNVCDNLADHMVGNVYVQFREE 120
             D     +    +Q+H++EF+E++F E+  KYGE+E +NVCDNL DH+VGNVYV+FR E
Sbjct: 61  SADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRRE 120

Query: 121 EQAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGRE 180
           E A  A+ +L+ R++ G+PI  + SPVTDFREA CRQYE   C RGG+CNFMHLK I RE
Sbjct: 121 EDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRE 180

Query: 181 LRHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSR 235
           LR EL+   R++H   RSRSRS  R     +R  G  G      ERD    SR R
Sbjct: 181 LRRELYGRRRKKH---RSRSRSRERRSRSRDRGRGGGGGGGGGRERDR-RRSRDR 231

[Close]

BLAST of Cucsat.G6547 vs. NCBI nr
Match: XP_004139466.1 (splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing factor U2af small subunit B [Cucumis sativus] >KGN64894.1 hypothetical protein Csa_022738 [Cucumis sativus])

HSP 1 Score: 648 bits (1671), Expect = 2.05e-234
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS
Sbjct: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS
Sbjct: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           EVKYANQTCGYEEQQQRQPPEQGYGY
Sbjct: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326

[Close]

BLAST of Cucsat.G6547 vs. NCBI nr
Match: XP_008461981.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_008461988.1 PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0065889.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa] >TYK15128.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 630 bits (1625), Expect = 2.10e-227
Identity = 317/326 (97.24%), Postives = 321/326 (98.47%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFAMYRRR SHSRSRSRSPYRHRSYEERSYG+HGHSRRYDERDAYHE+RSRR RTTS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSP REGSEERRAKIEQWN+EREQGNDNNA SDDNRN+HEKSYDS
Sbjct: 241 PGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQGNDNNAKSDDNRNSHEKSYDS 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           EVKYANQTCGYEEQQQRQPPEQGYGY
Sbjct: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326

[Close]

BLAST of Cucsat.G6547 vs. NCBI nr
Match: XP_038894442.1 (splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894443.1 splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894444.1 splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894445.1 splicing factor U2af small subunit B-like [Benincasa hispida])

HSP 1 Score: 605 bits (1559), Expect = 2.32e-217
Identity = 305/326 (93.56%), Postives = 315/326 (96.63%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRI REL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFA+YRRRHS SRSRSRSPYRHRSYEERSYG+HGHSRRYDERDAY+ESR  RHRTTS
Sbjct: 181 RHELFAIYRRRHSRSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYYESRGMRHRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSPVREGSEE RAKIEQWN+EREQGNDNN NSD N+N+HEKSY  
Sbjct: 241 PGHRSRSRSPRGRKNRSPVREGSEESRAKIEQWNREREQGNDNNGNSD-NKNSHEKSYSG 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           +VKY+NQTCGYEEQ+Q+ PPEQGYGY
Sbjct: 301 DVKYSNQTCGYEEQRQQLPPEQGYGY 325

[Close]

BLAST of Cucsat.G6547 vs. NCBI nr
Match: XP_023519661.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519662.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519664.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 585 bits (1509), Expect = 1.16e-209
Identity = 300/330 (90.91%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRI REL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFA Y RR SHSRSRSRSPYRHRSYEER YG+HGHSRRYDERDAYH+SR+RRHR+TS
Sbjct: 181 RHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGHR-SRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG-NDNNANSDDNRNNHEKSY 300
           PGHR SRSRSPRGRKNRSPVREGSEERRAKIEQWN+E+EQ  N NNANSD N N+HEKSY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQENYNNANSDGNENSHEKSY 300

Query: 301 DSEVKYANQTCGYEEQQQRQ--PPEQGYGY 326
           + +V+Y NQTCGY++QQQ+Q  PPEQGYGY
Sbjct: 301 NGDVQYENQTCGYKQQQQQQQKPPEQGYGY 330

[Close]

BLAST of Cucsat.G6547 vs. NCBI nr
Match: XP_022970323.1 (splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970326.1 splicing factor U2af small subunit B-like [Cucurbita maxima])

HSP 1 Score: 584 bits (1505), Expect = 4.57e-209
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRI REL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFA Y RR  HSRSRSRSPYRHRSYEER YG+HGHSRRYDERDAYH+SR+RRHR+TS
Sbjct: 181 RHELFASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGHR-SRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYD 300
           PGHR SRSRSPRGRKNRSPVRE SEERRAKIEQWN+E+EQ N NNANSD N ++HEKSY+
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREESEERRAKIEQWNREKEQENYNNANSDGNEHSHEKSYN 300

Query: 301 SEVKYANQTCGY--EEQQQRQPPEQGYGY 326
            +V+Y NQTCGY  ++QQQ+QPPEQGYGY
Sbjct: 301 GDVQYENQTCGYKQQQQQQQQPPEQGYGY 329

[Close]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL
Match: A0A0A0LVA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1)

HSP 1 Score: 648 bits (1671), Expect = 9.90e-235
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS
Sbjct: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS
Sbjct: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           EVKYANQTCGYEEQQQRQPPEQGYGY
Sbjct: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326

[Close]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL
Match: A0A1S3CFU2 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103500459 PE=4 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 1.02e-227
Identity = 317/326 (97.24%), Postives = 321/326 (98.47%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFAMYRRR SHSRSRSRSPYRHRSYEERSYG+HGHSRRYDERDAYHE+RSRR RTTS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSP REGSEERRAKIEQWN+EREQGNDNNA SDDNRN+HEKSYDS
Sbjct: 241 PGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQGNDNNAKSDDNRNSHEKSYDS 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           EVKYANQTCGYEEQQQRQPPEQGYGY
Sbjct: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326

[Close]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL
Match: A0A5D3CV58 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00620 PE=4 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 1.02e-227
Identity = 317/326 (97.24%), Postives = 321/326 (98.47%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFAMYRRR SHSRSRSRSPYRHRSYEERSYG+HGHSRRYDERDAYHE+RSRR RTTS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDS 300
           PGHRSRSRSPRGRKNRSP REGSEERRAKIEQWN+EREQGNDNNA SDDNRN+HEKSYDS
Sbjct: 241 PGHRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQGNDNNAKSDDNRNSHEKSYDS 300

Query: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326
           EVKYANQTCGYEEQQQRQPPEQGYGY
Sbjct: 301 EVKYANQTCGYEEQQQRQPPEQGYGY 326

[Close]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL
Match: A0A6J1I2J7 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111469321 PE=4 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 2.21e-209
Identity = 297/329 (90.27%), Postives = 312/329 (94.83%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRI REL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFA Y RR  HSRSRSRSPYRHRSYEER YG+HGHSRRYDERDAYH+SR+RRHR+TS
Sbjct: 181 RHELFASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGHR-SRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYD 300
           PGHR SRSRSPRGRKNRSPVRE SEERRAKIEQWN+E+EQ N NNANSD N ++HEKSY+
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREESEERRAKIEQWNREKEQENYNNANSDGNEHSHEKSYN 300

Query: 301 SEVKYANQTCGY--EEQQQRQPPEQGYGY 326
            +V+Y NQTCGY  ++QQQ+QPPEQGYGY
Sbjct: 301 GDVQYENQTCGYKQQQQQQQQPPEQGYGY 329

[Close]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL
Match: A0A6J1HM65 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111464850 PE=4 SV=1)

HSP 1 Score: 582 bits (1501), Expect = 9.33e-209
Identity = 298/330 (90.30%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRI REL
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240
           RHELFA Y RR SHSRSRSRSPYRHRSYEER YG+HGHSRRYDERDAYH+SR+RRHR+TS
Sbjct: 181 RHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGHR-SRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG-NDNNANSDDNRNNHEKSY 300
           PGHR SRSRSPRGRKNRSPVREGSEERRAKIEQWN+E+EQ  N NN+NSD + N+HEKSY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQKNYNNSNSDGHENSHEKSY 300

Query: 301 DSEVKYANQTCGYEEQQQRQ--PPEQGYGY 326
           + +V+Y NQTCGY++QQQ+Q  PPEQGYGY
Sbjct: 301 NGDVQYENQTCGYKQQQQQQQKPPEQGYGY 330

[Close]

BLAST of Cucsat.G6547 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.1e-107
Identity = 205/293 (69.97%), Postives = 231/293 (78.84%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP  IQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
            AA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+I REL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RHELFAMYR---RRHSHSRSRSRSPYRHRSY-EERSYG------KHGHSRRYDERDAYHE 240
           R +LF  YR   RR S SRSRS SP R R +  ER  G      +HG+ +R  +R   H+
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 ---SRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG 281
                 RRH     G   RSRSPR       VREGSEERRA+IEQWN+ER++G
Sbjct: 241 RDGGGRRRH-----GSPKRSRSPRN------VREGSEERRARIEQWNRERDEG 282

[Close]

BLAST of Cucsat.G6547 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.1e-107
Identity = 205/293 (69.97%), Postives = 231/293 (78.84%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP  IQDHFE+FYED+F+ELNK+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
            AA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K+I REL
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 RHELFAMYR---RRHSHSRSRSRSPYRHRSY-EERSYG------KHGHSRRYDERDAYHE 240
           R +LF  YR   RR S SRSRS SP R R +  ER  G      +HG+ +R  +R   H+
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

Query: 241 ---SRSRRHRTTSPGHRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQG 281
                 RRH     G   RSRSPR       VREGSEERRA+IEQWN+ER++G
Sbjct: 241 RDGGGRRRH-----GSPKRSRSPRN------VREGSEERRARIEQWNRERDEG 282

[Close]

BLAST of Cucsat.G6547 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 378.6 bits (971), Expect = 4.9e-105
Identity = 198/298 (66.44%), Postives = 227/298 (76.17%), Query Frame = 0


Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPR IQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180
           QAA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNFMH+K + REL
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 RHELFAMYRRRH-----SHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRR 240
           R +LF  YRR +     S SRSRS SP   R  + R       S R  +R+ Y     + 
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGK- 240

Query: 241 HRTTSPGHRSRSRSPRGRKNRSPV--------REGSEERRAKIEQWNKEREQGNDNNA 286
            R++    R      RGR+  SP         REGSEERRA+IEQWN+ERE+  +  A
Sbjct: 241 -RSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNREREEKEEGGA 296

[Close]

BLAST of Cucsat.G6547 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 275.0 bits (702), Expect = 7.7e-74
Identity = 151/248 (60.89%), Postives = 178/248 (71.77%), Query Frame = 0


Query: 51  MYQRPDMITPGVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVG 110
           MYQRPDMITPGVDAQG P+DPR IQ+HFE+F+EDLF+EL K+GEIESLN+CDNLADHM+G
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEEQAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNF 170
           NVYVQF+EE+QAA AL+ L GRFY+GRPII DFSPVTDFREATCRQYEEN CNRGGYCNF
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 MHLKRIGRELRHELFAMYRRRH-----SHSRSRSRSPYRHRSYEERSYGKHGHSRRYDER 230
           MH+K + RELR +LF  YRR +     S SRSRS SP   R  + R       S R  +R
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

Query: 231 DAYHESRSRRHRTTSPGHRSRSRSPRGRKNRSPV--------REGSEERRAKIEQWNKER 286
           + Y     +  R++    R      RGR+  SP         REGSEERRA+IEQWN+ER
Sbjct: 181 EFYRHGSGK--RSSERSERQERDGSRGRRQASPKRGGSPGGGREGSEERRARIEQWNRER 240

[Close]

BLAST of Cucsat.G6547 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 107.5 bits (267), Expect = 2.1e-23
Identity = 98/323 (30.34%), Postives = 154/323 (47.68%), Query Frame = 0


Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNP 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P+ S TLL+ NMY  P  IT   D +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPG-ITWEQD-EGLE 296

Query: 69  IDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRN 128
                 +  +EEFYED+  E  KYGE+ +  VC N + H+ GNVYV +R  E A  A ++
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LSGRFYAGRPIIVDFSPVTDFREATCRQYEEN---MCNRGGYCNFMH-LKRIGRELRHEL 188
           ++GR++AG+ +  +F  ++ ++ A C +Y ++    C+RG  CNF+H  +  G +     
Sbjct: 357 INGRYFAGKQVNCEFVNISRWKVAICGEYMKSRLKTCSRGSACNFIHCFRNPGGDYEWAD 416

Query: 189 FAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTSPGHR 248
                 R    +  S   Y    + E       +    D     H   SRR R+    H 
Sbjct: 417 HDRPPPRFWIHKMTSLFGYSDEKHMEHESSGSLNDSISDLSTDSHRQPSRRSRSRDHDHA 476

Query: 249 SRSRSP--RGRKNRSPVREGSEERRAKIEQWNKEREQGNDNNANSDDNRNNHEKSYDSEV 308
           +   +P  R RK     ++ + E     ++  +  E  +D + +S     + E+    E 
Sbjct: 477 NVGSTPSYRSRKYHGDTQDSTRE-----DKLRRHAENCHDGD-DSPSRDGSLEREMYKER 536

Query: 309 KYANQTCGYEEQQQRQPPEQGYG 326
           +YA  T   + +     P    G
Sbjct: 537 RYAKDTLHRDSRWSEHSPGHRVG 551

The following BLAST results are available for this feature:

BLAST of Cucsat.G6547 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQW8	6.7e-107	69.73	Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q9FMY5	1.5e-106	69.97	Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ...	[more]
Q9S709	6.9e-104	66.44	Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ...	[more]
Q6AUG0	5.1e-99	63.49	Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
A1A4K8	1.3e-65	55.32	Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1	[more]

BLAST of Cucsat.G6547 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139466.1	2.05e-234	100.00	splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing ...	[more]
XP_008461981.1	2.10e-227	97.24	PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_00846198...	[more]
XP_038894442.1	2.32e-217	93.56	splicing factor U2af small subunit B-like [Benincasa hispida] >XP_038894443.1 sp...	[more]
XP_023519661.1	1.16e-209	90.91	splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022970323.1	4.57e-209	90.27	splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 spl...	[more]

BLAST of Cucsat.G6547 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVA4	9.90e-235	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1	[more]
A0A1S3CFU2	1.02e-227	97.24	splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10350045...	[more]
A0A5D3CV58	1.02e-227	97.24	Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1I2J7	2.21e-209	90.27	splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1HM65	9.33e-209	90.30	splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Cucsat.G6547 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42820.2	1.1e-107	69.97	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT5G42820.1	1.1e-107	69.97	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT1G27650.1	4.9e-105	66.44	U2 snRNP auxiliary factor small subunit, putative	[more]
AT1G27650.2	7.7e-74	60.89	U2 snRNP auxiliary factor small subunit, putative	[more]
AT1G10320.1	2.1e-23	30.34	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009145	U2 auxiliary factor small subunit	PRINTS	PR01848	U2AUXFACTOR	coord: 8..32 score: 54.36 coord: 43..55 score: 63.64
IPR009145	U2 auxiliary factor small subunit	PANTHER	PTHR12620	U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT	coord: 9..185
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..204
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 132..179
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 114..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..113
None	No IPR available	PANTHER	PTHR12620:SF39	SPLICING FACTOR U2AF SMALL SUBUNIT A	coord: 9..185
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 26..53 score: 10.774557

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G6547.T1	Cucsat.G6547.T1	mRNA
Cucsat.G6547.T2	Cucsat.G6547.T2	mRNA
Cucsat.G6547.T3	Cucsat.G6547.T3	mRNA
Cucsat.G6547.T4	Cucsat.G6547.T4	mRNA
Cucsat.G6547.T5	Cucsat.G6547.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0005681	spliceosomal complex
cellular_component	GO:0089701	U2AF complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030628	pre-mRNA 3'-splice site binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding