Cucsat.G6344 (gene) Cucumber (B10) v3

Overview
NameCucsat.G6344
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionproton pump-interactor 2-like isoform X1
Locationctg1429: 656048 .. 658659 (-)
RNA-Seq ExpressionCucsat.G6344
SyntenyCucsat.G6344
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGGCTACCATCTTCTCCCATTCACAGCACCAAGTTCAGACACCCATTGTTTTGATTTTGAGGTATATCAAACCCCTTGTCTATTTTTCTTGGTCTGTTTTTCATCCTTAAGTTTGAATTTTTCTTTAGTTATGAAGCTTTGACATTGCCACTGTAATGTGAATTAGTTTTAGTTTACACACAAAACTAGAGCCCGATGCTTTAGTTATGAACTGGGTGCAATTTCTTTCAGTGTTTTTGAAAGGTTTTTTTATATTTAGTTTGAAGTATGTTTCTGCATTAGGCAAAGAAAAGCACCATGGAAGAAGGTGATCAATTCAATTGTAGAGAGAAGAATATGGTGGAAGAACAAGAAGGATCGGAAGTAATTGGTGAAGAGGGGATAATAGATTGTTCTGATGATGAAATGCCTGTTGGAGTTGTTCGACATTTTTATTTTGTGAAGGTTTTGCCACTTGAGAACCCAAATTTGGATGCCATGATCAAGAAGGCTGAAGAGATGATTGACAAGACGAATCGAGACCAAGTTCTGCTAGCCACAAAGATACGGGAAAAGATGGTATGTGAAAAATAAACCATAGCCTTTCTTTTCAGAACGTTTTACTCAACTCTCCAGCTGGTTGGCACTAGCCCATGTGAGCCCTCAACAATGGAAGATTGTAACTTTTCATTCATTGGACAAAAATCGGATTTCCTTTATGAACTGATAGTGGTATAAAACACATTGAATTTAGTGAATTCTGTTTGTAGATGGATCGCGACGCGGTGCGCTCAAAATTGAGCTGGATGAAGAAGTATGATTATTATGAACTCACCATAAAATGGCATAAGGAGAGATTGGACATCCTGCATCTATCACTAGACAAACTGACCTTTGCAAACAACGCATATAAAGGAAAGCCGGTCAACTCGTGCTTGTCGTCAGGGGAAGTCGATAAGCAAGTATTGTCAATTGAACTCTTTCTCGTGCATTTCTGTGACGCTCTCGATCTTGTTTGTCTTATGTAATAGATTTATCATGTGAGCAGAAACTGCATTTCTTGATGCTACATGGCTGTAAAAACATGGCTGATGAGAGAAAGCTATTAAGGGAAGTCAATGCAAGCCAAGGGAAGGATGGGGGCATCACATTAGATGAACTTCATGCTCCTGTAATATTAAACCTTGAGCATTAATAGAATATTCAATTCTACTTCTCGTTTTTTATCCTTTGATCTTTAGTACATGAATTATGTTCTTTCTTAAGTTTCTTGGTTCTCATGAACAGATTCAGCGCTTGCGAGGACAATTTCCCACCAACTACATGGGGCGTACAGAATCCGATGAAAATGCTCGTAAACAAGCCATTCTCAAAGGCATAAAACATCATGAAGTTATTATGGAGAAAGCCATTGCTAATGCCGTAGTGAATGGAAAATTGTGGAACTCTCTAAGCTCAAAAAAGTCCATTCAAGAAGAAGTAAGAACCAAAAATAAAATGTAGATTTTTTTTTTTTTTCTGGCATACAACTATAACTAAAAAATTTGTGATGTTGAATGTTGCAGATAGAGATGCTCAACAACAGTTCAATTCAACCAAGGGAAAGACAAAGGAAAACAATAACCGAAATTCGGAAGGTAAATTTAGAACTCGAGAAGGTAGAAAAAGATATACGTTCTCTACAAAAGCTCTTCACAGATGCAAATCGCAAAAAAGATGACGCATACACAACCATTCTTAGACTGAAGAAGCAGTATGGGGAAGAGGTATAAATGATAATTGGAAACTTATGAACTCTAATTTTGCACTTAAATCATGCTACATCAAGTATAGAAACCTGGCCCTGTCTACCGAGCATGTGAATAAACGAGAAATTTTGTTTGAAAAAGATAAATAATGCATATATCAGGTTGTTTTATAAGTTATATCTACTGATAGAAATGAAAATTCTGCTTTTTATTTTCAGTGTAGTCATTGATTTAGATCACCATATCCTGAATTGCATAATTATTTGCTGATTTCTCATGATGTAAGTTGAGTTTGAACTAATTTATCAATGTTTTTGGTTTTTTTTCGTGTAAAGAATGCTTCCTACTACCAATACCGTTCCCTCATGAAGAAAGTTGAAGCACTTGTTAAAAAGAAAGACATTGCAGCAGTTCAAGAAGTATCTCAAACACAGGTTATTCCAATATCCCTTGTTTATTGTTTCTTTGTTCTTTTTAGGGAAAGGATGTTATAATTAAACTCTCCATGATATTCATTGTTACATTAGCTATAGCTCTCCTTGTTCATAAACAGTTTTAGGATTTTGAGAACTTATGAAAACTAATATTTCCATTACAAACAGGTTGAAAAATTTATGCACCAGTGGAATGATAACATGGAATTCAGAAACGATTACAAAAAGAGAGTCAACCCGTCGTTAAAAAACCGACATCTTGGTGTGGATGGGAGGATGACGATGAGCAACCAAAAGCCAGAGGTTGAGGACACTCGGAAAATTATTAAACCAGATACTCTTTCTAAAACTAGATTGAAATGGTTGATGAAAGATAGTGAGGATCCCTTTGAACTTCTTTCTTAATGAAACTAATGCAATAAATGTTTGTTTTTCTCGTCCCTTTGTTA

Coding sequence (CDS)

ATGGATCGCGACGCGGTGCGCTCAAAATTGAGCTGGATGAAGAAGTATGATTATTATGAACTCACCATAAAATGGCATAAGGAGAGATTGGACATCCTGCATCTATCACTAGACAAACTGACCTTTGCAAACAACGCATATAAAGGAAAGCCGGTCAACTCGTGCTTGTCGTCAGGGGAAGTCGATAAGCAAAAACTGCATTTCTTGATGCTACATGGCTGTAAAAACATGGCTGATGAGAGAAAGCTATTAAGGGAAGTCAATGCAAGCCAAGGGAAGGATGGGGGCATCACATTAGATGAACTTCATGCTCCTATTCAGCGCTTGCGAGGACAATTTCCCACCAACTACATGGGGCGTACAGAATCCGATGAAAATGCTCGTAAACAAGCCATTCTCAAAGGCATAAAACATCATGAAGTTATTATGGAGAAAGCCATTGCTAATGCCGTAGTGAATGGAAAATTGTGGAACTCTCTAAGCTCAAAAAAGTCCATTCAAGAAGAAATAGAGATGCTCAACAACAGTTCAATTCAACCAAGGGAAAGACAAAGGAAAACAATAACCGAAATTCGGAAGGTAAATTTAGAACTCGAGAAGGTAGAAAAAGATATACGTTCTCTACAAAAGCTCTTCACAGATGCAAATCGCAAAAAAGATGACGCATACACAACCATTCTTAGACTGAAGAAGCAGTATGGGGAAGAGAATGCTTCCTACTACCAATACCGTTCCCTCATGAAGAAAGTTGAAGCACTTGTTAAAAAGAAAGACATTGCAGCAGTTCAAGAAGTATCTCAAACACAGGTTGAAAAATTTATGCACCAGTGGAATGATAACATGGAATTCAGAAACGATTACAAAAAGAGAGTCAACCCGTCGTTAAAAAACCGACATCTTGGTGTGGATGGGAGGATGACGATGAGCAACCAAAAGCCAGAGGTTGAGGACACTCGGAAAATTATTAAACCAGATACTCTTTCTAAAACTAGATTGAAATGGTTGATGAAAGATAGTGAGGATCCCTTTGAACTTCTTTCTTAA

Protein sequence

MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS
Homology
BLAST of Cucsat.G6344 vs. ExPASy Swiss-Prot
Match: Q762B4 (Proton pump-interactor BIP103 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP103 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.7e-21
Identity = 69/245 (28.16%), Postives = 136/245 (55.51%), Query Frame = 0

Query: 82  KLLREVNASQGKDGGI--TLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHH 141
           K   E NA + +  GI  +++EL   I+RL  +         ES     ++ ++K I+  
Sbjct: 162 KFRDENNAIRAQGAGICSSIEELEKSIKRLNDRI------SHESIPLDEEKRLIKQIREL 221

Query: 142 EVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELE 201
           E    K I+ +    ++ +++  + +IQ++++++       ++ ++   ++I+ +  EL+
Sbjct: 222 EKTRPKVISTSANRAQIQDTVVERDAIQDQVKIIGEGIDGVKKERQAVRSKIKVLEDELK 281

Query: 202 KVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDI 261
            ++ ++ SLQ+  T AN +KD A+ ++++L+      NAS++Q R L+ K   L  + ++
Sbjct: 282 AIDMEMGSLQEDLTAANARKDKAHESLVQLRHARDAYNASFHQNRQLLSKARDLASRSEL 341

Query: 262 AAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTR 321
           A VQE+ +TQV+KF+ +W ++  FR DY+KR+  SL +R L  DGRM   ++KP   +T 
Sbjct: 342 AQVQELYKTQVDKFVAEWCNSKAFREDYEKRILSSLNSRQLSRDGRMRNPDEKPIFIETE 400

Query: 322 KIIKP 325
               P
Sbjct: 402 AAAPP 400

BLAST of Cucsat.G6344 vs. ExPASy Swiss-Prot
Match: O23144 (Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 7.8e-21
Identity = 85/306 (27.78%), Postives = 149/306 (48.69%), Query Frame = 0

Query: 27  KERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLM------LHGCKNMADE 86
           K +LD+    L+KL  A      K     L +   ++ +L  L+        G   M DE
Sbjct: 93  KAKLDLADKELEKLNKARTGVLDK-----LRAKRAERSELFDLLDPLKSERKGFNTMFDE 152

Query: 87  -RKLLREVNASQGK----DGG--------ITLDELHAPIQRLRGQFPTNYMGRTESDENA 146
            RK +  +  + GK    DGG         + +EL++ I      +   Y  + ES    
Sbjct: 153 KRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMI------YSYQYRIQHESIPLT 212

Query: 147 RKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKT 206
            ++ ILK I+  E   +K IANA +  K+  S+  K  IQ +++++       ++ ++  
Sbjct: 213 EEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQAI 272

Query: 207 ITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLM 266
              I +++ +L+  + +I  L+      + K+D AY+ I  L++Q  E N+ YYQ R+++
Sbjct: 273 SARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSEYYQNRTVL 332

Query: 267 KKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMT 314
            K   L  +K+I+ ++ ++  +VEKF+  W     FR DY+KR+  SL +R L  DGRM 
Sbjct: 333 NKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQLSRDGRMR 387

BLAST of Cucsat.G6344 vs. ExPASy Swiss-Prot
Match: Q6ZBF6 (Proton pump-interactor BIP131 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP131 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 5.6e-19
Identity = 68/240 (28.33%), Postives = 125/240 (52.08%), Query Frame = 0

Query: 82  KLLREVNASQGKDGGI--TLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHH 141
           K   E NA + +  G+  +++EL   I+ L  +         ES     ++ ++K IK  
Sbjct: 159 KFRSENNAMRAQGAGLCSSIEELDQLIKSLNDRI------SHESISLDEEKRLVKEIKQL 218

Query: 142 EVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELE 201
                K I NA    K+ +++  + +I ++++ +     + +  ++    +I+ +  ++ 
Sbjct: 219 NGTRSKVIENAAKRAKMQDTVVERGTIHDQVKQIGVGIDEVKRDRQAVRDKIKVLEDQIH 278

Query: 202 KVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDI 261
            V+ +I +LQ   T A  +KD A+  +  L+K     N S++QYR++   V  L  + ++
Sbjct: 279 AVDGEIAALQDDLTAATARKDKAFEALNELRKTRDLNNTSFHQYRTISNSVRDLSARGEV 338

Query: 262 AAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTR 320
            AVQ++ Q +VEKFM QW  +  FR DY+KR+  SL +R L  DGRM   ++KP V +T+
Sbjct: 339 EAVQQLCQNEVEKFMAQWCSSKSFREDYEKRILVSLNSRQLSRDGRMRNPDEKPIVLETQ 392

BLAST of Cucsat.G6344 vs. ExPASy Swiss-Prot
Match: Q8VZN4 (Proton pump-interactor 4 OS=Arabidopsis thaliana OX=3702 GN=PPI4 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-09
Identity = 47/195 (24.10%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 152 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTIT-EIRKVNLELEKVEKDIRSLQK 211
           +  +L +  +++ S+   +E L +  I   E++++ +   I ++   L +++ +I  L  
Sbjct: 86  IANRLKSKRAARLSLVASMETLMSMKINEVEKEQEVVRGRIYRLGERLSEIKMEIELLDV 145

Query: 212 LFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQV 271
                  ++D A   I  L+ Q  + NA++YQ R +MKK   L    ++  ++E++ ++V
Sbjct: 146 QMACVLNQRDKAVERIKFLRMQRDKGNAAFYQSRVVMKKAIELAASGNVRDLEELADSEV 205

Query: 272 EKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKT 331
           EKFM +WN++  FR++YKKR+ PS+  R L  D ++       + E+  + +    +   
Sbjct: 206 EKFMSRWNNDKAFRDNYKKRILPSVNERKLRCDVQIRDLEGNLDTENGNETVVKKAIEYK 265

Query: 332 RLKWLMKDSEDPFEL 346
           R     ++  D F++
Sbjct: 266 RFS--TEEESDDFDI 278

BLAST of Cucsat.G6344 vs. ExPASy Swiss-Prot
Match: B3H4K7 (Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.3e-09
Identity = 71/308 (23.05%), Postives = 127/308 (41.23%), Query Frame = 0

Query: 11  SWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDK--QKLHF 70
           S + K + Y + I+  K   D LH SL  L  + +         C S  E+D      H+
Sbjct: 123 SLVSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSD------QLCFSKEELDHLIYIAHY 182

Query: 71  LMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENAR 130
            + HG     +E  +L+E      K  GI L E                      D  A 
Sbjct: 183 QIEHGSIGFEEEDWVLKETE----KADGIVLSE----------------------DSLAE 242

Query: 131 KQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTI 190
           K+A +  +K   V + +                    +++E++ +               
Sbjct: 243 KEASINRVKSMAVELNE--------------------VKKELDAIT-------------- 302

Query: 191 TEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMK 250
            +I  ++ ++ K + ++R L         ++D +Y  I  L+ Q  +  A++YQ   +M+
Sbjct: 303 WKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMR 360

Query: 251 KVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTM 310
           K   L    ++  ++  + ++ ++FM QWND+  FR+DY KR++PSL  R L  DGR+  
Sbjct: 363 KARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRI-- 360

Query: 311 SNQKPEVE 317
             + PEV+
Sbjct: 423 --KDPEVQ 360

BLAST of Cucsat.G6344 vs. NCBI nr
Match: XP_011656800.2 (proton pump-interactor BIP131 [Cucumis sativus] >KGN46450.1 hypothetical protein Csa_005555 [Cucumis sativus])

HSP 1 Score: 676 bits (1745), Expect = 4.24e-243
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE
Sbjct: 118 MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 177

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR
Sbjct: 178 VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 237

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
           TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP
Sbjct: 238 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 297

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY
Sbjct: 298 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 357

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL
Sbjct: 358 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 417

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           GVDGRMTM+NQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS
Sbjct: 418 GVDGRMTMNNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 464

BLAST of Cucsat.G6344 vs. NCBI nr
Match: XP_008459867.1 (PREDICTED: proton pump-interactor 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 556 bits (1433), Expect = 2.77e-196
Identity = 296/347 (85.30%), Postives = 308/347 (88.76%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WM+ YDYYELTIKW KERLDILHL LDKLTFANNAYK KPVNSCLSSGE
Sbjct: 86  MDRDAVRSKLRWMRNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKPVNSCLSSGE 145

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDK+KLHFLMLHGCKNMADERKLLREVN SQGKDGGITLDELHAPI+RLR QFP  + G 
Sbjct: 146 VDKRKLHFLMLHGCKNMADERKLLREVNESQGKDGGITLDELHAPIRRLRQQFPMYFRG- 205

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
            ESDE+ RKQ        HEVI+EKAIANAVVNGKLWNSLSSKKSIQEEIE L N+S Q 
Sbjct: 206 -ESDEDPRKQ--------HEVIIEKAIANAVVNGKLWNSLSSKKSIQEEIEKLKNTSFQL 265

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRKT  EIRKV LELEKVEKDIRSLQKLFTDANRKK DAY TILRLKKQYGEENASY
Sbjct: 266 RERQRKTNAEIRKVKLELEKVEKDIRSLQKLFTDANRKKADAYKTILRLKKQYGEENASY 325

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           Y+YRSLMKKVEALVKKKDI  VQE+SQ QV KFM QWNDN+EFRNDYKKRVNPSL NRHL
Sbjct: 326 YKYRSLMKKVEALVKKKDITEVQELSQKQVGKFMQQWNDNLEFRNDYKKRVNPSLTNRHL 385

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           GVDGRM MSNQKPEVEDTRK+IKP+TLSK RLKWLMKD EDPFELLS
Sbjct: 386 GVDGRM-MSNQKPEVEDTRKVIKPETLSKARLKWLMKDLEDPFELLS 421

BLAST of Cucsat.G6344 vs. NCBI nr
Match: XP_038875496.1 (proton pump-interactor BIP131-like isoform X2 [Benincasa hispida])

HSP 1 Score: 545 bits (1403), Expect = 1.56e-191
Identity = 284/347 (81.84%), Postives = 310/347 (89.34%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRD +RSKLSW+K YDYY LT+KW KERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE
Sbjct: 90  MDRDTLRSKLSWVKNYDYYGLTLKWQKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 149

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDKQKL FLMLHGCKNMADERKLLREVNA QGKDGG+TL+ELHAPIQRLR Q    Y+GR
Sbjct: 150 VDKQKLSFLMLHGCKNMADERKLLREVNACQGKDGGMTLEELHAPIQRLRQQLSIKYVGR 209

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
           TE+DE+ARKQAILK IK HEV  EKAIANAVV GKLWNSL SK+S++EEI  L NSS + 
Sbjct: 210 TETDEDARKQAILKDIKQHEVNREKAIANAVVKGKLWNSLVSKESLKEEI--LKNSSYEL 269

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRK   EIRKVN+ELEKVEKDIRSLQKLFTDANRKKD AY TILRLKKQYGEENASY
Sbjct: 270 RERQRKVNAEIRKVNVELEKVEKDIRSLQKLFTDANRKKDGAYNTILRLKKQYGEENASY 329

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           YQYRSLMKKVE+L +KKDIAA+QE+SQ QVE+FM QWNDN++FRNDYKKRV PSLKN+HL
Sbjct: 330 YQYRSLMKKVESLAQKKDIAAIQELSQIQVEQFMKQWNDNLDFRNDYKKRVVPSLKNQHL 389

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           G DGRM ++NQKP VEDTRK+IKP+ LSK+RLKWLMKD EDP ELLS
Sbjct: 390 GFDGRM-ITNQKPAVEDTRKVIKPEALSKSRLKWLMKDLEDPSELLS 433

BLAST of Cucsat.G6344 vs. NCBI nr
Match: XP_038875495.1 (proton pump-interactor BIP131-like isoform X1 [Benincasa hispida])

HSP 1 Score: 545 bits (1403), Expect = 4.67e-191
Identity = 284/347 (81.84%), Postives = 310/347 (89.34%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRD +RSKLSW+K YDYY LT+KW KERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE
Sbjct: 121 MDRDTLRSKLSWVKNYDYYGLTLKWQKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 180

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDKQKL FLMLHGCKNMADERKLLREVNA QGKDGG+TL+ELHAPIQRLR Q    Y+GR
Sbjct: 181 VDKQKLSFLMLHGCKNMADERKLLREVNACQGKDGGMTLEELHAPIQRLRQQLSIKYVGR 240

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
           TE+DE+ARKQAILK IK HEV  EKAIANAVV GKLWNSL SK+S++EEI  L NSS + 
Sbjct: 241 TETDEDARKQAILKDIKQHEVNREKAIANAVVKGKLWNSLVSKESLKEEI--LKNSSYEL 300

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRK   EIRKVN+ELEKVEKDIRSLQKLFTDANRKKD AY TILRLKKQYGEENASY
Sbjct: 301 RERQRKVNAEIRKVNVELEKVEKDIRSLQKLFTDANRKKDGAYNTILRLKKQYGEENASY 360

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           YQYRSLMKKVE+L +KKDIAA+QE+SQ QVE+FM QWNDN++FRNDYKKRV PSLKN+HL
Sbjct: 361 YQYRSLMKKVESLAQKKDIAAIQELSQIQVEQFMKQWNDNLDFRNDYKKRVVPSLKNQHL 420

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           G DGRM ++NQKP VEDTRK+IKP+ LSK+RLKWLMKD EDP ELLS
Sbjct: 421 GFDGRM-ITNQKPAVEDTRKVIKPEALSKSRLKWLMKDLEDPSELLS 464

BLAST of Cucsat.G6344 vs. NCBI nr
Match: XP_008459865.1 (PREDICTED: proton pump-interactor 2-like isoform X1 [Cucumis melo] >XP_008459866.1 PREDICTED: proton pump-interactor 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 542 bits (1397), Expect = 2.33e-190
Identity = 297/376 (78.99%), Postives = 308/376 (81.91%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WM+ YDYYELTIKW KERLDILHL LDKLTFANNAYK KPVNSCLSSGE
Sbjct: 86  MDRDAVRSKLRWMRNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKPVNSCLSSGE 145

Query: 61  VDK-----------------------------QKLHFLMLHGCKNMADERKLLREVNASQ 120
           VDK                             QKLHFLMLHGCKNMADERKLLREVN SQ
Sbjct: 146 VDKRVLSIELFLVHFCDALLLICLYVIDLSCEQKLHFLMLHGCKNMADERKLLREVNESQ 205

Query: 121 GKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIANAV 180
           GKDGGITLDELHAPI+RLR QFP  + G  ESDE+ RKQ        HEVI+EKAIANAV
Sbjct: 206 GKDGGITLDELHAPIRRLRQQFPMYFRG--ESDEDPRKQ--------HEVIIEKAIANAV 265

Query: 181 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKL 240
           VNGKLWNSLSSKKSIQEEIE L N+S Q RERQRKT  EIRKV LELEKVEKDIRSLQKL
Sbjct: 266 VNGKLWNSLSSKKSIQEEIEKLKNTSFQLRERQRKTNAEIRKVKLELEKVEKDIRSLQKL 325

Query: 241 FTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQVE 300
           FTDANRKK DAY TILRLKKQYGEENASYY+YRSLMKKVEALVKKKDI  VQE+SQ QV 
Sbjct: 326 FTDANRKKADAYKTILRLKKQYGEENASYYKYRSLMKKVEALVKKKDITEVQELSQKQVG 385

Query: 301 KFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKTR 347
           KFM QWNDN+EFRNDYKKRVNPSL NRHLGVDGRM MSNQKPEVEDTRK+IKP+TLSK R
Sbjct: 386 KFMQQWNDNLEFRNDYKKRVNPSLTNRHLGVDGRM-MSNQKPEVEDTRKVIKPETLSKAR 445

BLAST of Cucsat.G6344 vs. ExPASy TrEMBL
Match: A0A0A0K9L8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G094680 PE=4 SV=1)

HSP 1 Score: 676 bits (1745), Expect = 2.05e-243
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE
Sbjct: 118 MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 177

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR
Sbjct: 178 VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 237

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
           TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP
Sbjct: 238 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 297

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY
Sbjct: 298 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 357

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL
Sbjct: 358 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 417

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           GVDGRMTM+NQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS
Sbjct: 418 GVDGRMTMNNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 464

BLAST of Cucsat.G6344 vs. ExPASy TrEMBL
Match: A0A1S3CB97 (proton pump-interactor 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498860 PE=4 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 1.34e-196
Identity = 296/347 (85.30%), Postives = 308/347 (88.76%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WM+ YDYYELTIKW KERLDILHL LDKLTFANNAYK KPVNSCLSSGE
Sbjct: 86  MDRDAVRSKLRWMRNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKPVNSCLSSGE 145

Query: 61  VDKQKLHFLMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGR 120
           VDK+KLHFLMLHGCKNMADERKLLREVN SQGKDGGITLDELHAPI+RLR QFP  + G 
Sbjct: 146 VDKRKLHFLMLHGCKNMADERKLLREVNESQGKDGGITLDELHAPIRRLRQQFPMYFRG- 205

Query: 121 TESDENARKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQP 180
            ESDE+ RKQ        HEVI+EKAIANAVVNGKLWNSLSSKKSIQEEIE L N+S Q 
Sbjct: 206 -ESDEDPRKQ--------HEVIIEKAIANAVVNGKLWNSLSSKKSIQEEIEKLKNTSFQL 265

Query: 181 RERQRKTITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASY 240
           RERQRKT  EIRKV LELEKVEKDIRSLQKLFTDANRKK DAY TILRLKKQYGEENASY
Sbjct: 266 RERQRKTNAEIRKVKLELEKVEKDIRSLQKLFTDANRKKADAYKTILRLKKQYGEENASY 325

Query: 241 YQYRSLMKKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHL 300
           Y+YRSLMKKVEALVKKKDI  VQE+SQ QV KFM QWNDN+EFRNDYKKRVNPSL NRHL
Sbjct: 326 YKYRSLMKKVEALVKKKDITEVQELSQKQVGKFMQQWNDNLEFRNDYKKRVNPSLTNRHL 385

Query: 301 GVDGRMTMSNQKPEVEDTRKIIKPDTLSKTRLKWLMKDSEDPFELLS 347
           GVDGRM MSNQKPEVEDTRK+IKP+TLSK RLKWLMKD EDPFELLS
Sbjct: 386 GVDGRM-MSNQKPEVEDTRKVIKPETLSKARLKWLMKDLEDPFELLS 421

BLAST of Cucsat.G6344 vs. ExPASy TrEMBL
Match: A0A1S3CCE0 (proton pump-interactor 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498860 PE=4 SV=1)

HSP 1 Score: 542 bits (1397), Expect = 1.13e-190
Identity = 297/376 (78.99%), Postives = 308/376 (81.91%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WM+ YDYYELTIKW KERLDILHL LDKLTFANNAYK KPVNSCLSSGE
Sbjct: 86  MDRDAVRSKLRWMRNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKPVNSCLSSGE 145

Query: 61  VDK-----------------------------QKLHFLMLHGCKNMADERKLLREVNASQ 120
           VDK                             QKLHFLMLHGCKNMADERKLLREVN SQ
Sbjct: 146 VDKRVLSIELFLVHFCDALLLICLYVIDLSCEQKLHFLMLHGCKNMADERKLLREVNESQ 205

Query: 121 GKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIANAV 180
           GKDGGITLDELHAPI+RLR QFP  + G  ESDE+ RKQ        HEVI+EKAIANAV
Sbjct: 206 GKDGGITLDELHAPIRRLRQQFPMYFRG--ESDEDPRKQ--------HEVIIEKAIANAV 265

Query: 181 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKL 240
           VNGKLWNSLSSKKSIQEEIE L N+S Q RERQRKT  EIRKV LELEKVEKDIRSLQKL
Sbjct: 266 VNGKLWNSLSSKKSIQEEIEKLKNTSFQLRERQRKTNAEIRKVKLELEKVEKDIRSLQKL 325

Query: 241 FTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQVE 300
           FTDANRKK DAY TILRLKKQYGEENASYY+YRSLMKKVEALVKKKDI  VQE+SQ QV 
Sbjct: 326 FTDANRKKADAYKTILRLKKQYGEENASYYKYRSLMKKVEALVKKKDITEVQELSQKQVG 385

Query: 301 KFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKTR 347
           KFM QWNDN+EFRNDYKKRVNPSL NRHLGVDGRM MSNQKPEVEDTRK+IKP+TLSK R
Sbjct: 386 KFMQQWNDNLEFRNDYKKRVNPSLTNRHLGVDGRM-MSNQKPEVEDTRKVIKPETLSKAR 445

BLAST of Cucsat.G6344 vs. ExPASy TrEMBL
Match: A0A5A7T8N4 (Proton pump-interactor 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G00990 PE=4 SV=1)

HSP 1 Score: 541 bits (1395), Expect = 2.27e-190
Identity = 298/376 (79.26%), Postives = 307/376 (81.65%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WMK YDYYELTIKW KERLDILHL LDKLTFANNAYK K VNSCLSSGE
Sbjct: 86  MDRDAVRSKLRWMKNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKQVNSCLSSGE 145

Query: 61  VDK-----------------------------QKLHFLMLHGCKNMADERKLLREVNASQ 120
           VDK                             QKLHFLMLHGCKNMADERKLLREVN SQ
Sbjct: 146 VDKRVLSIELFLVHFCDALLLICLYVIDLSCEQKLHFLMLHGCKNMADERKLLREVNESQ 205

Query: 121 GKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIANAV 180
           GKDGGITLDELHAPI+RLR QFP  + G  ESDE+ RKQ        HEVI+EKAIANAV
Sbjct: 206 GKDGGITLDELHAPIRRLRQQFPMYFRG--ESDEDPRKQ--------HEVIIEKAIANAV 265

Query: 181 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKL 240
           VNGKLWNSLSSKKSIQEEIE L NSS Q RERQRKT  EIRKV LELEKVEKDIRSLQKL
Sbjct: 266 VNGKLWNSLSSKKSIQEEIEKLKNSSFQLRERQRKTNAEIRKVKLELEKVEKDIRSLQKL 325

Query: 241 FTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQVE 300
           FTDANRKK DAY TILRLKKQYGEENASYY+YRSLMKKVEALVKKKDI  VQE+SQ QV 
Sbjct: 326 FTDANRKKADAYKTILRLKKQYGEENASYYKYRSLMKKVEALVKKKDITEVQELSQKQVG 385

Query: 301 KFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKTR 347
           KFM QWNDN+EFRNDYKKRVNPSL NRHLGVDGRM MSNQKPEVEDTRK+IKP+TLSK R
Sbjct: 386 KFMQQWNDNLEFRNDYKKRVNPSLTNRHLGVDGRM-MSNQKPEVEDTRKVIKPETLSKAR 445

BLAST of Cucsat.G6344 vs. ExPASy TrEMBL
Match: A0A5D3DM53 (Proton pump-interactor 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002240 PE=4 SV=1)

HSP 1 Score: 541 bits (1395), Expect = 7.55e-187
Identity = 298/376 (79.26%), Postives = 307/376 (81.65%), Query Frame = 0

Query: 1   MDRDAVRSKLSWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGE 60
           MDRDAVRSKL WMK YDYYELTIKW KERLDILHL LDKLTFANNAYK K VNSCLSSGE
Sbjct: 326 MDRDAVRSKLRWMKNYDYYELTIKWQKERLDILHLYLDKLTFANNAYKEKQVNSCLSSGE 385

Query: 61  VDK-----------------------------QKLHFLMLHGCKNMADERKLLREVNASQ 120
           VDK                             QKLHFLMLHGCKNMADERKLLREVN SQ
Sbjct: 386 VDKRVLSIELFLVHFCDALLLICLYVIDLSCEQKLHFLMLHGCKNMADERKLLREVNESQ 445

Query: 121 GKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIANAV 180
           GKDGGITLDELHAPI+RLR QFP  + G  ESDE+ RKQ        HEVI+EKAIANAV
Sbjct: 446 GKDGGITLDELHAPIRRLRQQFPMYFRG--ESDEDPRKQ--------HEVIIEKAIANAV 505

Query: 181 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKL 240
           VNGKLWNSLSSKKSIQEEIE L NSS Q RERQRKT  EIRKV LELEKVEKDIRSLQKL
Sbjct: 506 VNGKLWNSLSSKKSIQEEIEKLKNSSFQLRERQRKTNAEIRKVKLELEKVEKDIRSLQKL 565

Query: 241 FTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQVE 300
           FTDANRKK DAY TILRLKKQYGEENASYY+YRSLMKKVEALVKKKDI  VQE+SQ QV 
Sbjct: 566 FTDANRKKADAYKTILRLKKQYGEENASYYKYRSLMKKVEALVKKKDITEVQELSQKQVG 625

Query: 301 KFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKTR 347
           KFM QWNDN+EFRNDYKKRVNPSL NRHLGVDGRM MSNQKPEVEDTRK+IKP+TLSK R
Sbjct: 626 KFMQQWNDNLEFRNDYKKRVNPSLTNRHLGVDGRM-MSNQKPEVEDTRKVIKPETLSKAR 685

BLAST of Cucsat.G6344 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 102.8 bits (255), Expect = 5.6e-22
Identity = 85/306 (27.78%), Postives = 149/306 (48.69%), Query Frame = 0

Query: 27  KERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLM------LHGCKNMADE 86
           K +LD+    L+KL  A      K     L +   ++ +L  L+        G   M DE
Sbjct: 93  KAKLDLADKELEKLNKARTGVLDK-----LRAKRAERSELFDLLDPLKSERKGFNTMFDE 152

Query: 87  -RKLLREVNASQGK----DGG--------ITLDELHAPIQRLRGQFPTNYMGRTESDENA 146
            RK +  +  + GK    DGG         + +EL++ I      +   Y  + ES    
Sbjct: 153 KRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMI------YSYQYRIQHESIPLT 212

Query: 147 RKQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKT 206
            ++ ILK I+  E   +K IANA +  K+  S+  K  IQ +++++       ++ ++  
Sbjct: 213 EEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQAI 272

Query: 207 ITEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLM 266
              I +++ +L+  + +I  L+      + K+D AY+ I  L++Q  E N+ YYQ R+++
Sbjct: 273 SARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSEYYQNRTVL 332

Query: 267 KKVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMT 314
            K   L  +K+I+ ++ ++  +VEKF+  W     FR DY+KR+  SL +R L  DGRM 
Sbjct: 333 NKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQLSRDGRMR 387

BLAST of Cucsat.G6344 vs. TAIR 10
Match: AT1G53110.1 (BEST Arabidopsis thaliana protein match is: proton pump interactor 2 (TAIR:AT3G15340.1); Has 9980 Blast hits to 7312 proteins in 825 species: Archae - 146; Bacteria - 1301; Metazoa - 3338; Fungi - 830; Plants - 588; Viruses - 42; Other Eukaryotes - 3735 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 1.3e-10
Identity = 47/195 (24.10%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 152 VNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTIT-EIRKVNLELEKVEKDIRSLQK 211
           +  +L +  +++ S+   +E L +  I   E++++ +   I ++   L +++ +I  L  
Sbjct: 86  IANRLKSKRAARLSLVASMETLMSMKINEVEKEQEVVRGRIYRLGERLSEIKMEIELLDV 145

Query: 212 LFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQTQV 271
                  ++D A   I  L+ Q  + NA++YQ R +MKK   L    ++  ++E++ ++V
Sbjct: 146 QMACVLNQRDKAVERIKFLRMQRDKGNAAFYQSRVVMKKAIELAASGNVRDLEELADSEV 205

Query: 272 EKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMSNQKPEVEDTRKIIKPDTLSKT 331
           EKFM +WN++  FR++YKKR+ PS+  R L  D ++       + E+  + +    +   
Sbjct: 206 EKFMSRWNNDKAFRDNYKKRILPSVNERKLRCDVQIRDLEGNLDTENGNETVVKKAIEYK 265

Query: 332 RLKWLMKDSEDPFEL 346
           R     ++  D F++
Sbjct: 266 RFS--TEEESDDFDI 278

BLAST of Cucsat.G6344 vs. TAIR 10
Match: AT1G10880.1 (Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 55/194 (28.35%), Postives = 92/194 (47.42%), Query Frame = 0

Query: 37  LDKLTFANNAYKGKPVNSCLSSG--EVDKQKLHFLMLHGCKNM-ADERKLLREVNASQGK 96
           LDKLTFANNAY+ K      +SG  E++   L+  MLH    M   ER+LL+ +N ++  
Sbjct: 451 LDKLTFANNAYRDKKAVISFASGEREINIHNLNHQMLHNAHCMDGGERRLLKMLNPNKDI 510

Query: 97  DGGITLDELHAPIQRLRGQFPTNYMGRTESDEN-ARKQAILKGIKHHEVIMEKAIANAVV 156
           D G +L    A ++  +  +       T  +E  +RKQ      +  E   ++   NA  
Sbjct: 511 DSGSSL----AQVEEQKWPYLNTTSTITIDEETYSRKQ------REFECERDQVFVNAPS 570

Query: 157 NGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSLQKLF 216
              LW SL S K ++++I+ +     Q R++      +I     +++K E +I+S++K  
Sbjct: 571 KASLWKSLPSTKDLRDQIQAMELKGEQKRKKVVGRRKKIESRERKIKKTENEIKSMRKTM 630

Query: 217 TDANRKKDDAYTTI 227
                +   A  TI
Sbjct: 631 ERILNRMHKALETI 634

BLAST of Cucsat.G6344 vs. TAIR 10
Match: AT3G15340.1 (proton pump interactor 2 )

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 71/308 (23.05%), Postives = 127/308 (41.23%), Query Frame = 0

Query: 11  SWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDK--QKLHF 70
           S + K + Y + I+  K   D LH SL  L  + +         C S  E+D      H+
Sbjct: 123 SLVSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSD------QLCFSKEELDHLIYIAHY 182

Query: 71  LMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENAR 130
            + HG     +E  +L+E      K  GI L E                      D  A 
Sbjct: 183 QIEHGSIGFEEEDWVLKETE----KADGIVLSE----------------------DSLAE 242

Query: 131 KQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTI 190
           K+A +  +K   V + +                    +++E++ +               
Sbjct: 243 KEASINRVKSMAVELNE--------------------VKKELDAIT-------------- 302

Query: 191 TEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMK 250
            +I  ++ ++ K + ++R L         ++D +Y  I  L+ Q  +  A++YQ   +M+
Sbjct: 303 WKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMR 360

Query: 251 KVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTM 310
           K   L    ++  ++  + ++ ++FM QWND+  FR+DY KR++PSL  R L  DGR+  
Sbjct: 363 KARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRI-- 360

Query: 311 SNQKPEVE 317
             + PEV+
Sbjct: 423 --KDPEVQ 360

BLAST of Cucsat.G6344 vs. TAIR 10
Match: AT3G15340.2 (proton pump interactor 2 )

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 71/308 (23.05%), Postives = 127/308 (41.23%), Query Frame = 0

Query: 11  SWMKKYDYYELTIKWHKERLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDK--QKLHF 70
           S + K + Y + I+  K   D LH SL  L  + +         C S  E+D      H+
Sbjct: 3   SLVSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSD------QLCFSKEELDHLIYIAHY 62

Query: 71  LMLHGCKNMADERKLLREVNASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENAR 130
            + HG     +E  +L+E      K  GI L E                      D  A 
Sbjct: 63  QIEHGSIGFEEEDWVLKETE----KADGIVLSE----------------------DSLAE 122

Query: 131 KQAILKGIKHHEVIMEKAIANAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTI 190
           K+A +  +K   V + +                    +++E++ +               
Sbjct: 123 KEASINRVKSMAVELNE--------------------VKKELDAIT-------------- 182

Query: 191 TEIRKVNLELEKVEKDIRSLQKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMK 250
            +I  ++ ++ K + ++R L         ++D +Y  I  L+ Q  +  A++YQ   +M+
Sbjct: 183 WKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMR 240

Query: 251 KVEALVKKKDIAAVQEVSQTQVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTM 310
           K   L    ++  ++  + ++ ++FM QWND+  FR+DY KR++PSL  R L  DGR+  
Sbjct: 243 KARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRI-- 240

Query: 311 SNQKPEVE 317
             + PEV+
Sbjct: 303 --KDPEVQ 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q762B42.7e-2128.16Proton pump-interactor BIP103 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP103... [more]
O231447.8e-2127.78Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2[more]
Q6ZBF65.6e-1928.33Proton pump-interactor BIP131 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP131... [more]
Q8VZN41.8e-0924.10Proton pump-interactor 4 OS=Arabidopsis thaliana OX=3702 GN=PPI4 PE=2 SV=1[more]
B3H4K75.3e-0923.05Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011656800.24.24e-24399.71proton pump-interactor BIP131 [Cucumis sativus] >KGN46450.1 hypothetical protein... [more]
XP_008459867.12.77e-19685.30PREDICTED: proton pump-interactor 2-like isoform X2 [Cucumis melo][more]
XP_038875496.11.56e-19181.84proton pump-interactor BIP131-like isoform X2 [Benincasa hispida][more]
XP_038875495.14.67e-19181.84proton pump-interactor BIP131-like isoform X1 [Benincasa hispida][more]
XP_008459865.12.33e-19078.99PREDICTED: proton pump-interactor 2-like isoform X1 [Cucumis melo] >XP_008459866... [more]
Match NameE-valueIdentityDescription
A0A0A0K9L82.05e-24399.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G094680 PE=4 SV=1[more]
A0A1S3CB971.34e-19685.30proton pump-interactor 2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498860... [more]
A0A1S3CCE01.13e-19078.99proton pump-interactor 2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498860... [more]
A0A5A7T8N42.27e-19079.26Proton pump-interactor 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3DM537.55e-18779.26Proton pump-interactor 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
AT4G27500.15.6e-2227.78proton pump interactor 1 [more]
AT1G53110.11.3e-1024.10BEST Arabidopsis thaliana protein match is: proton pump interactor 2 (TAIR:AT3G1... [more]
AT1G10880.13.7e-1028.35Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein [more]
AT3G15340.13.7e-1023.05proton pump interactor 2 [more]
AT3G15340.23.7e-1023.05proton pump interactor 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 304..331
NoneNo IPR availablePANTHERPTHR32219RNA-BINDING PROTEIN YLMH-RELATEDcoord: 22..437
IPR029669Proton pump-interactorPANTHERPTHR32219:SF2PROTON PUMP-INTERACTOR 1coord: 22..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G6344.T2Cucsat.G6344.T2mRNA
Cucsat.G6344.T3Cucsat.G6344.T3mRNA
Cucsat.G6344.T1Cucsat.G6344.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010155 regulation of proton transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane