Cucsat.G6325 (gene) Cucumber (B10) v3

BLAST of Cucsat.G6325 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 7.4e-24
Identity = 101/381 (26.51%), Postives = 188/381 (49.34%), Query Frame = 0

Query: 42  IYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLLPMEREKHK---AFLHFKARNNLYLES 101
           I+++P+    +NPKAY+PK+VS+GPYHYG+ HL  +++ K +    FL    + ++    
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEENV 107

Query: 102 IVDSVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIVDGCFVLDLI------SELSSKSL 161
           +V +V  + + +  SY + L  +   D+ MF  +M++DGCF+L +        ELS   +
Sbjct: 108 LVKAVVDLEDKIRKSYSEEL--KTGHDL-MF--MMVLDGCFILMVFLIMSGNIELSEDPI 167

Query: 162 GRC--MIWDIKRDMVLLENQLPLQLLKQLHALTTTNENE----------KENLDLEAMIW 221
                ++  I+ D++LLENQ+P  +L+ L+  +    +           K  +D E   W
Sbjct: 168 FSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAFHFFKNPIDKEGSYW 227

Query: 222 SWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWTLTNEENVEYCQS 281
                  E        H+LD+ R + L P   + D+     + + +       NV    S
Sbjct: 228 -------EKHRNYKAKHLLDLIRETFL-PNTSESDKASSPHVQV-QLHEGKSGNVPSVDS 287

Query: 282 N----IRSATQLRKAGIKF--EKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINI 341
                I SA +LR  GIKF   +S  +++++V    K+  L++P L  D  ++    +N 
Sbjct: 288 KAVPLILSAKRLRLQGIKFRLRRSKEDSILNVR--LKKNKLQIPQLRFDGFISS-FFLNC 347

Query: 342 MAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQLFNVLAK 395
           +AFE+ +     ++T+++V M  L+  E+DV+ L +  ++  N     N V + F  ++K
Sbjct: 348 VAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISK 407

BLAST of Cucsat.G6325 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.8e-15
Identity = 109/503 (21.67%), Postives = 200/503 (39.76%), Query Frame = 0

Query: 7   EEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGP 66
           +E   V+N   +L ++LEE          + +  SI+ +P+ +   +P +Y P  VS+GP
Sbjct: 17  DETRWVINVQKSLDAELEEH-------DLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGP 76

Query: 67  YHYGKPHLLPMEREKHKAFLHFKAR---NNLYLESIVDSVCSILEDLLGSYDDNLDDRWK 126
           YH  KP L  MER  +K  +  K R   N+     +V+ + S+   +   Y   +    +
Sbjct: 77  YHCLKPELHEMER--YKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGE 136

Query: 127 EDVAMFLKLMIVDGCFVLDLISELSSKS----LGRCMIWDIKRDMVLLENQLPLQLLKQL 186
                 L +M VD  F+++ +   S +     + R    +I RD++++ENQ+PL +L++ 
Sbjct: 137 ----TLLWIMAVDSSFLIEFLKIYSFRKVETLINRVGHNEILRDIMMIENQIPLFVLRKT 196

Query: 187 HALTTTNENEKENLDLEAMIWSWMGLSGEIL----------SMRSPLHILDIYRSSLLSP 246
                 +    ++L L  +      LS  ++            +   HILD     ++  
Sbjct: 197 LEFQLESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIV-- 256

Query: 247 TRCKQDETHE-----------------------------------NTITILEW------- 306
            R +++E  E                                   + I    W       
Sbjct: 257 PRIEEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNLP 316

Query: 307 --------------------TLTNEENVEYC---------QSNIRSATQLRKAGIKFEKS 366
                               T T +E+V            +  I S + L KAG++F+ +
Sbjct: 317 GFMALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPT 376

Query: 367 STNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNL 422
           +  N+  V+FD       LP + + DI TE  L N++A+E  +       T +  L+  +
Sbjct: 377 AHGNISTVTFDSNSGQFYLPVINL-DINTETVLRNLVAYEATNTSGPLVFTRYTELINGI 436

BLAST of Cucsat.G6325 vs. NCBI nr
Match: XP_004140657.1 (UPF0481 protein At3g47200 [Cucumis sativus] >KAE8646751.1 hypothetical protein Csa_005047 [Cucumis sativus])

HSP 1 Score: 879 bits (2272), Expect = 0.0
Identity = 446/446 (100.00%), Postives = 446/446 (100.00%), Query Frame = 0

Query: 1   MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK 60
           MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK
Sbjct: 1   MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK 60

Query: 61  LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD 120
           LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD
Sbjct: 61  LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD 120

Query: 121 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 180
           RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH
Sbjct: 121 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 180

Query: 181 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 240
           ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN
Sbjct: 181 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 240

Query: 241 TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL 300
           TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL
Sbjct: 241 TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL 300

Query: 301 AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH 360
           AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH
Sbjct: 301 AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH 360

Query: 361 DGNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQII 420
           DGNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQII
Sbjct: 361 DGNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQII 420

Query: 421 SIALFTFGFTYPFVQAITDKRHRRKQ 446
           SIALFTFGFTYPFVQAITDKRHRRKQ
Sbjct: 421 SIALFTFGFTYPFVQAITDKRHRRKQ 446

BLAST of Cucsat.G6325 vs. NCBI nr
Match: XP_016902479.1 (PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo])

HSP 1 Score: 793 bits (2049), Expect = 2.49e-280
Identity = 411/445 (92.36%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 4   MKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQH-IKPSIYKIPQFIREVNPKAYEPKLV 63
           MKQEEMGGVVNEVN LLSKLEESIGMEEFLSTQH IKPSIYKIPQFIREVNPKAYEPKLV
Sbjct: 1   MKQEEMGGVVNEVNELLSKLEESIGMEEFLSTQHMIKPSIYKIPQFIREVNPKAYEPKLV 60

Query: 64  SLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRW 123
           SLGPYHYGKPHLLPMEREK+KAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD W
Sbjct: 61  SLGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDIW 120

Query: 124 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 183
           KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL
Sbjct: 121 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 180

Query: 184 TTTN-ENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENT 243
           TTT  ENE E+LDLEAMIWSWM LSGEILS+R+PLHILD+YRSSLLSPT CKQDETHEN 
Sbjct: 181 TTTTTENENEHLDLEAMIWSWMRLSGEILSVRNPLHILDMYRSSLLSPTICKQDETHENM 240

Query: 244 ITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA 303
           I ILEWTLTNE+NVE CQS IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA
Sbjct: 241 IAILEWTLTNEDNVE-CQS-IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA 300

Query: 304 IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHD 363
           IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIG++K+VSLLASKGILSSNVIHD
Sbjct: 301 IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKNVSLLASKGILSSNVIHD 360

Query: 364 GNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIIS 423
           GNGVVQLFN+LAKGQTKYLE+HM ELF+MLNCY+DNRWSIWLENLKLKVPQTKN W+ IS
Sbjct: 361 GNGVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEFIS 420

Query: 424 IALFTFGFTYPFVQAITDKRHRRKQ 446
           IA FTFGFTYP VQAITD R R+KQ
Sbjct: 421 IAFFTFGFTYPLVQAITDNRRRKKQ 443

BLAST of Cucsat.G6325 vs. NCBI nr
Match: KAA0039868.1 (UPF0481 protein [Cucumis melo var. makuwa] >TYK24633.1 UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 790 bits (2039), Expect = 4.49e-279
Identity = 409/440 (92.95%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 9   MGGVVNEVNALLSKLEESIGMEEFLSTQH-IKPSIYKIPQFIREVNPKAYEPKLVSLGPY 68
           MGGVVNEVN LLSKLEESIGMEEFLSTQH IKPSIYKIPQFIREVNPKAYEPKLVSLGPY
Sbjct: 1   MGGVVNEVNELLSKLEESIGMEEFLSTQHMIKPSIYKIPQFIREVNPKAYEPKLVSLGPY 60

Query: 69  HYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVA 128
           HYGKPHLLPMEREK+KAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD WKEDVA
Sbjct: 61  HYGKPHLLPMEREKYKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDIWKEDVA 120

Query: 129 MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTN- 188
           MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTT  
Sbjct: 121 MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTTT 180

Query: 189 ENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILE 248
           ENE E+LDLEAMIWSWM LSGEILSMR+PLHILD+YRSSLLSPT CKQDETHEN I ILE
Sbjct: 181 ENENEHLDLEAMIWSWMRLSGEILSMRNPLHILDMYRSSLLSPTICKQDETHENMIAILE 240

Query: 249 WTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL 308
           WTLTNE+NVE CQS IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL
Sbjct: 241 WTLTNEDNVE-CQS-IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL 300

Query: 309 TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVV 368
           TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIG++KDVSLLASKGILSSNVIHDGNGVV
Sbjct: 301 TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKDVSLLASKGILSSNVIHDGNGVV 360

Query: 369 QLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFT 428
           QLFN+LAKGQTKYLE+HM ELF+MLNCY+DNRWSIWLENLKLKVPQTKN W+IISIA FT
Sbjct: 361 QLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEIISIAFFT 420

Query: 429 FGFTYPFVQAITDKRHRRKQ 446
           FGFTYP VQAITD R R+KQ
Sbjct: 421 FGFTYPLVQAITDNRRRKKQ 438

BLAST of Cucsat.G6325 vs. NCBI nr
Match: XP_038875622.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 308 bits (788), Expect = 9.51e-97
Identity = 198/428 (46.26%), Postives = 275/428 (64.25%), Query Frame = 0

Query: 12  VVNEVNALLSKLEESIGMEEFLST-QHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYG 71
           VV +V   L+KL +S  ++E     +  +PSIYKIP+F+R++  KA+EP+LVSLGPYH+G
Sbjct: 24  VVIQVKNNLNKLLDSAAVDEMGPLGESTQPSIYKIPEFMRKIQAKAFEPQLVSLGPYHHG 83

Query: 72  KPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAMFL 131
           K HL+ ME EK KAF  F     L LESIV+S+ + LE+L G+YD  LD++WK+D A FL
Sbjct: 84  KEHLVSMEEEKQKAFRRFVNDYGLNLESIVESMSNNLENLWGAYD-KLDEKWKQDGAKFL 143

Query: 132 KLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHA-LTTTNENE 191
           ++MIVDGCF+LD   E    SL   M WDIKRDM+LLENQLP+QLL++L+  +  TN+ +
Sbjct: 144 EVMIVDGCFMLDFFKE-CPYSLST-MRWDIKRDMLLLENQLPMQLLQELYDNMPNTNQED 203

Query: 192 KENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWTL 251
           +    L ++I   M ++ E +  R  LHILD+YR+SLL P   ++D + +  +T      
Sbjct: 204 QS---LRSLICQPMCINEEAVMGRE-LHILDMYRASLLYPPVDRKDRSGKTKVTY----- 263

Query: 252 TNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEP 311
             +++   CQ  I  ATQL  AGIKF+ S T NL DVSF+ KQ VL LP + +DD  TE 
Sbjct: 264 --QQSDPECQI-IPQATQLHDAGIKFKSSPTKNLTDVSFNPKQGVLELPYIVVDDD-TET 323

Query: 312 TLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQLF 371
           TL+N+MAFEKL+V    +VTSFV+LM NLI +++DV+LLAS  IL+ N + +      LF
Sbjct: 324 TLLNVMAFEKLYVEAGSKVTSFVILMNNLIDVDRDVALLASDKILA-NALGNDESAADLF 383

Query: 372 NVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFTFGF 431
           ++L KG    L+SH+ ++   +N +    W+ W  +LK    Q  N W IIS+    FGF
Sbjct: 384 SLLGKGAAMDLDSHITDVHYKVNKHCSLSWNQWCASLKHDYFQ--NPWAIISLFAAVFGF 432

Query: 432 TYPFVQAI 437
               VQAI
Sbjct: 444 AILIVQAI 432

BLAST of Cucsat.G6325 vs. NCBI nr
Match: XP_022960454.1 (UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 269 bits (688), Expect = 7.72e-82
Identity = 181/429 (42.19%), Postives = 262/429 (61.07%), Query Frame = 0

Query: 12  VVNEVNALLSKLEES---IGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYH 71
           +V  V   L +L +S   + ME   S++ +  SIYKIP F+ + +PKAYEP++VSLGPY+
Sbjct: 23  MVVRVKGTLDQLLDSPAILNMEAEQSSELL--SIYKIPFFMTQTHPKAYEPQVVSLGPYN 82

Query: 72  YGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAM 131
           +GK HL PME EK K F  FKAR  L +ESIV  V +IL++L+ SYD  L++ WKED   
Sbjct: 83  HGKQHLSPMELEKLKLFHSFKARCLLDVESIVRGVSTILDELMESYD-KLEEDWKEDPGK 142

Query: 132 FLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNEN 191
           FL+LMIVDGCF+L  +       +   +  DIK+DM+LLENQLP+ LL++L+++   N  
Sbjct: 143 FLQLMIVDGCFMLGFLINCPDSLIN--VSPDIKQDMLLLENQLPMLLLEKLYSIADRNVQ 202

Query: 192 EKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWT 251
             +  DL+ ++ +W+ +    + M+  LHIL++Y+ SLL P   + D           W+
Sbjct: 203 LPQQ-DLKKLVSNWLNIPRNEV-MKDCLHILEMYKESLLHPPIDRTD-----------WS 262

Query: 252 LTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTE 311
           +    +   CQ  I  AT+LR+AGIKF++S T +L DV FD K  VL LP L +DD  TE
Sbjct: 263 VELGHSDPECQV-IPPATKLREAGIKFKRSKTGSLTDVFFDAKGGVLWLPRLMVDDN-TE 322

Query: 312 PTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQL 371
            TL+N+MAFEKLH+   R+VTSFV+LM NLI  E+DV++LA + +L+ N + +      L
Sbjct: 323 STLLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEQVLA-NAVGNDKEAAGL 382

Query: 372 FNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFTFG 431
           FN L  G    L++HM  + + +N + +  W+     LK +  Q+   W IIS+    FG
Sbjct: 383 FNRLGSGAAMGLDTHMAWVHKKVNEHCNQPWNERCATLKHEYFQSP--WTIISLCAAIFG 428

Query: 432 FTYPFVQAI 437
           F    +QAI
Sbjct: 443 FIILILQAI 428

BLAST of Cucsat.G6325 vs. ExPASy TrEMBL
Match: A0A0A0KAA3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G091270 PE=4 SV=1)

HSP 1 Score: 818 bits (2114), Expect = 2.70e-294
Identity = 423/441 (95.92%), Postives = 425/441 (96.37%), Query Frame = 0

Query: 1   MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK 60
           MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK
Sbjct: 1   MEIMKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPK 60

Query: 61  LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD 120
           LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD
Sbjct: 61  LVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD 120

Query: 121 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 180
           RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH
Sbjct: 121 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 180

Query: 181 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 240
           ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN
Sbjct: 181 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 240

Query: 241 TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL 300
           TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL
Sbjct: 241 TITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSL 300

Query: 301 AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH 360
           AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH
Sbjct: 301 AIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIH 360

Query: 361 DGNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQII 420
           DGNGVVQLFNVLAKGQTKYLESH+                IWLENLKLKVPQTKNSWQII
Sbjct: 361 DGNGVVQLFNVLAKGQTKYLESHI----------------IWLENLKLKVPQTKNSWQII 420

Query: 421 SIALFTFGFTYPFVQAITDKR 441
           SIALFTFGFTYPFVQAITDK+
Sbjct: 421 SIALFTFGFTYPFVQAITDKQ 425

BLAST of Cucsat.G6325 vs. ExPASy TrEMBL
Match: A0A1S4E2L7 (uncharacterized protein LOC103499077 OS=Cucumis melo OX=3656 GN=LOC103499077 PE=4 SV=1)

HSP 1 Score: 793 bits (2049), Expect = 1.20e-280
Identity = 411/445 (92.36%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 4   MKQEEMGGVVNEVNALLSKLEESIGMEEFLSTQH-IKPSIYKIPQFIREVNPKAYEPKLV 63
           MKQEEMGGVVNEVN LLSKLEESIGMEEFLSTQH IKPSIYKIPQFIREVNPKAYEPKLV
Sbjct: 1   MKQEEMGGVVNEVNELLSKLEESIGMEEFLSTQHMIKPSIYKIPQFIREVNPKAYEPKLV 60

Query: 64  SLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRW 123
           SLGPYHYGKPHLLPMEREK+KAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD W
Sbjct: 61  SLGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDIW 120

Query: 124 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 183
           KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL
Sbjct: 121 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 180

Query: 184 TTTN-ENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENT 243
           TTT  ENE E+LDLEAMIWSWM LSGEILS+R+PLHILD+YRSSLLSPT CKQDETHEN 
Sbjct: 181 TTTTTENENEHLDLEAMIWSWMRLSGEILSVRNPLHILDMYRSSLLSPTICKQDETHENM 240

Query: 244 ITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA 303
           I ILEWTLTNE+NVE CQS IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA
Sbjct: 241 IAILEWTLTNEDNVE-CQS-IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLA 300

Query: 304 IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHD 363
           IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIG++K+VSLLASKGILSSNVIHD
Sbjct: 301 IDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKNVSLLASKGILSSNVIHD 360

Query: 364 GNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIIS 423
           GNGVVQLFN+LAKGQTKYLE+HM ELF+MLNCY+DNRWSIWLENLKLKVPQTKN W+ IS
Sbjct: 361 GNGVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEFIS 420

Query: 424 IALFTFGFTYPFVQAITDKRHRRKQ 446
           IA FTFGFTYP VQAITD R R+KQ
Sbjct: 421 IAFFTFGFTYPLVQAITDNRRRKKQ 443

BLAST of Cucsat.G6325 vs. ExPASy TrEMBL
Match: A0A5A7TA99 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001800 PE=4 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 2.17e-279
Identity = 409/440 (92.95%), Postives = 422/440 (95.91%), Query Frame = 0

Query: 9   MGGVVNEVNALLSKLEESIGMEEFLSTQH-IKPSIYKIPQFIREVNPKAYEPKLVSLGPY 68
           MGGVVNEVN LLSKLEESIGMEEFLSTQH IKPSIYKIPQFIREVNPKAYEPKLVSLGPY
Sbjct: 1   MGGVVNEVNELLSKLEESIGMEEFLSTQHMIKPSIYKIPQFIREVNPKAYEPKLVSLGPY 60

Query: 69  HYGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVA 128
           HYGKPHLLPMEREK+KAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDD WKEDVA
Sbjct: 61  HYGKPHLLPMEREKYKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDIWKEDVA 120

Query: 129 MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTN- 188
           MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTT  
Sbjct: 121 MFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTTT 180

Query: 189 ENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILE 248
           ENE E+LDLEAMIWSWM LSGEILSMR+PLHILD+YRSSLLSPT CKQDETHEN I ILE
Sbjct: 181 ENENEHLDLEAMIWSWMRLSGEILSMRNPLHILDMYRSSLLSPTICKQDETHENMIAILE 240

Query: 249 WTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL 308
           WTLTNE+NVE CQS IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL
Sbjct: 241 WTLTNEDNVE-CQS-IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL 300

Query: 309 TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVV 368
           TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIG++KDVSLLASKGILSSNVIHDGNGVV
Sbjct: 301 TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKDVSLLASKGILSSNVIHDGNGVV 360

Query: 369 QLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFT 428
           QLFN+LAKGQTKYLE+HM ELF+MLNCY+DNRWSIWLENLKLKVPQTKN W+IISIA FT
Sbjct: 361 QLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEIISIAFFT 420

Query: 429 FGFTYPFVQAITDKRHRRKQ 446
           FGFTYP VQAITD R R+KQ
Sbjct: 421 FGFTYPLVQAITDNRRRKKQ 438

BLAST of Cucsat.G6325 vs. ExPASy TrEMBL
Match: A0A6J1HB25 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=4 SV=1)

HSP 1 Score: 269 bits (688), Expect = 3.74e-82
Identity = 181/429 (42.19%), Postives = 262/429 (61.07%), Query Frame = 0

Query: 12  VVNEVNALLSKLEES---IGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYH 71
           +V  V   L +L +S   + ME   S++ +  SIYKIP F+ + +PKAYEP++VSLGPY+
Sbjct: 23  MVVRVKGTLDQLLDSPAILNMEAEQSSELL--SIYKIPFFMTQTHPKAYEPQVVSLGPYN 82

Query: 72  YGKPHLLPMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAM 131
           +GK HL PME EK K F  FKAR  L +ESIV  V +IL++L+ SYD  L++ WKED   
Sbjct: 83  HGKQHLSPMELEKLKLFHSFKARCLLDVESIVRGVSTILDELMESYD-KLEEDWKEDPGK 142

Query: 132 FLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNEN 191
           FL+LMIVDGCF+L  +       +   +  DIK+DM+LLENQLP+ LL++L+++   N  
Sbjct: 143 FLQLMIVDGCFMLGFLINCPDSLIN--VSPDIKQDMLLLENQLPMLLLEKLYSIADRNVQ 202

Query: 192 EKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWT 251
             +  DL+ ++ +W+ +    + M+  LHIL++Y+ SLL P   + D           W+
Sbjct: 203 LPQQ-DLKKLVSNWLNIPRNEV-MKDCLHILEMYKESLLHPPIDRTD-----------WS 262

Query: 252 LTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTE 311
           +    +   CQ  I  AT+LR+AGIKF++S T +L DV FD K  VL LP L +DD  TE
Sbjct: 263 VELGHSDPECQV-IPPATKLREAGIKFKRSKTGSLTDVFFDAKGGVLWLPRLMVDDN-TE 322

Query: 312 PTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQL 371
            TL+N+MAFEKLH+   R+VTSFV+LM NLI  E+DV++LA + +L+ N + +      L
Sbjct: 323 STLLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEQVLA-NAVGNDKEAAGL 382

Query: 372 FNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFTFG 431
           FN L  G    L++HM  + + +N + +  W+     LK +  Q+   W IIS+    FG
Sbjct: 383 FNRLGSGAAMGLDTHMAWVHKKVNEHCNQPWNERCATLKHEYFQSP--WTIISLCAAIFG 428

Query: 432 FTYPFVQAI 437
           F    +QAI
Sbjct: 443 FIILILQAI 428

BLAST of Cucsat.G6325 vs. ExPASy TrEMBL
Match: E5GB49 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 270 bits (689), Expect = 5.67e-82
Identity = 179/412 (43.45%), Postives = 242/412 (58.74%), Query Frame = 0

Query: 34  STQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLLPMEREKHKAFLHF-KARN 93
           S + IKPSIYKIP+F++++  KAYEP LVS GPYH+G  HL PME+EK K F H  K  N
Sbjct: 55  SEKTIKPSIYKIPKFMKDIQLKAYEPYLVSFGPYHHGVEHLAPMEKEKQKVFQHLVKGDN 114

Query: 94  NLYL-ESIVDSVCSILEDLLGSYDDNLDDRWKEDV---AMFLKLMIVDGCFVLDLISELS 153
           N    ESI   V +ILEDL  +YD NLD++W++DV   A F+++MI+D CF+L   S+  
Sbjct: 115 NAATYESIASEVSNILEDLYAAYD-NLDEKWRKDVVASAKFMEMMIIDACFILVFFSKDK 174

Query: 154 SKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNENEKENLDLEAMIWSWMGLSGE 213
           S      +  DIKRD++LLENQLP QLL+ L+ +    +  K    L   +W    +  +
Sbjct: 175 SYKSLMTLRSDIKRDILLLENQLPFQLLQLLYKILPIKDQNKSLTSLICKLWF---VKKD 234

Query: 214 ILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWTLTNEENVEYCQSN---IRSA 273
            L+++   HIL++YR  LL P      E  E+     E T  N++  +    N   I  A
Sbjct: 235 ELTVKGGKHILEMYRMLLLDPIPIVLSERDESQKKA-EGTKGNKKEKDESVLNSQIIPQA 294

Query: 274 TQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVE 333
           T L  AGIKF +S T +L+D+ F  K  VL LP L +DD  TE  L+N+MAFEKLH  V 
Sbjct: 295 TLLHDAGIKFRRSETESLIDIGF--KNGVLELPHLTVDDD-TETKLLNVMAFEKLHGNVR 354

Query: 334 RQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQLFNVLAKGQTKYLESHMY 393
             VTSFVVLM NLI ++KDV LL+   I+  N + +     +LF VL KG    LES++ 
Sbjct: 355 SYVTSFVVLMNNLIDIDKDVELLSENKIID-NALGNDEDAAKLFTVLGKGVALDLESNIA 414

Query: 394 ELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIALFTFGFTYPFVQAI 437
           ++ R++N + D R + W  NLK    Q  N W IIS+    FGF    VQA+
Sbjct: 415 KVHRLVNKHCDGRCNRWCANLKHNYFQ--NPWAIISLIGAIFGFLILIVQAV 455

BLAST of Cucsat.G6325 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-40
Identity = 119/409 (29.10%), Postives = 207/409 (50.61%), Query Frame = 0

Query: 42  IYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLESIVD 101
           IY++P +++E + K+Y P++VS+GPYH+G  HL+PMER K +A     AR    +E  +D
Sbjct: 106 IYRVPPYLQENDTKSYMPQIVSIGPYHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYID 165

Query: 102 SVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIVDGCFVLDLISELSS--KSLG------ 161
           ++  + E     Y   ++    E    F++++++DG F++++    S   + +G      
Sbjct: 166 AMKELEEKARACYQGPINMNRNE----FIEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDP 225

Query: 162 ----RCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNENEKENLDLEAMIWSWMGLSGEI 221
               R ++  I+RDMV+LENQLP  +LK L  L   +  +K N+ L    +  +  + E+
Sbjct: 226 VFGMRGLMQSIRRDMVMLENQLPWSVLKGLLQLQRPDVLDKVNVQLFQPFFQPLLPTREV 285

Query: 222 LSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILEWTLTNEENVEYCQSNIRSATQLR 281
           L+    LH LD+ R  LL  +    +          + ++ N++     Q  I   T+LR
Sbjct: 286 LTEEGGLHCLDVLRRGLLQSSGTSDE----------DMSMVNKQP----QQLIHCVTELR 345

Query: 282 KAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVERQVT 341
            AG++F +  T +  D+  +FK   L++P L I D  T+   +N++AFE+ H+   +++T
Sbjct: 346 NAGVEFMRKETGHFWDI--EFKNGYLKIPKLLIHD-GTKSLFLNLIAFEQCHIKSSKKIT 405

Query: 342 SFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQLFNVLAKGQT-KYLESHMYELF 401
           S+++ M NLI   +DVS L   GI+  N +   + V  LFN L K       + ++  L 
Sbjct: 406 SYIIFMDNLINSSEDVSYLHHYGII-ENWLGSDSEVSDLFNGLGKEVIFDPNDGYLSALT 465

Query: 402 RMLNCYHDNRWSIWLENLKLKVPQTKNSWQ----IISIALFTFGFTYPF 434
             +N Y+  +W+     L+ K     N W     I ++ L  F F   F
Sbjct: 466 GEVNIYYRRKWNYLKATLRHK--YFNNPWAYFSFIAAVTLLIFTFCQSF 490

BLAST of Cucsat.G6325 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 160.2 bits (404), Expect = 3.8e-39
Identity = 125/438 (28.54%), Postives = 211/438 (48.17%), Query Frame = 0

Query: 16  VNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLL 75
           V ++  K+E+++  +   S    K  IY++P +++E + K+Y P+ VS+GPYH+GK HL 
Sbjct: 68  VISIKDKMEKALSYDATNSWD--KLCIYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLR 127

Query: 76  PMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIV 135
           PMER K +A     AR    +E  +D++  + E+    Y   +D +   +   F +++++
Sbjct: 128 PMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEARACYQGPIDMKNSNE---FTEMLVL 187

Query: 136 DGCFVLDLISEL--SSKSLG----------RCMIWDIKRDMVLLENQLPLQLLKQLHALT 195
           DGCFVL+L        + +G          R ++  I+RDM++LENQLPL +L +L  L 
Sbjct: 188 DGCFVLELFKGTIQGFQKIGYARNDPVFAKRGLMHSIQRDMIMLENQLPLFVLDRLLGLQ 247

Query: 196 TTNENEKENL-DLEAMIWSWMGLSGEILSM----------------RSPLHILDIYRSSL 255
           T   N+   + ++    +  +  + E+L+                    LH LD++  SL
Sbjct: 248 TGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGGLHCLDVFHRSL 307

Query: 256 LSPTRCKQDETHENTITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDV 315
           +  +      T    +++          VE  Q  I   T+LR AG+ F +  T  L D+
Sbjct: 308 IQSSETTNQGTPYEDMSM----------VEKQQQLIHCVTELRGAGVNFMRKETGQLWDI 367

Query: 316 SFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVS 375
             +FK   L++P L I D  T+    N++AFE+ H      +TS+++ M NLI   +DVS
Sbjct: 368 --EFKNGYLKIPKLLIHD-GTKSLFSNLIAFEQCHTQSSNNITSYIIFMDNLINSSQDVS 427

Query: 376 LLASKGILSSNVIHDGNGVVQLFNVLAKGQT-KYLESHMYELFRMLNCYHDNRWSIWLEN 424
            L   GI+   +  D   V  LFN L K       + ++ +L R +N Y+  +W+     
Sbjct: 428 YLHHDGIIEHWLGSDSE-VADLFNRLCKEVIFDPKDGYLSQLSREVNRYYSRKWNSLKAT 484

BLAST of Cucsat.G6325 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 159.1 bits (401), Expect = 8.4e-39
Identity = 125/419 (29.83%), Postives = 201/419 (47.97%), Query Frame = 0

Query: 39  KPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLES 98
           K  IY++P +++E + K+Y P+ VSLGPYH+GK  L PMER K +A      R    +E 
Sbjct: 112 KLCIYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEM 171

Query: 99  IVDSVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIVDGCFVLDLI--SELSSKSLG--- 158
             +++  + E     Y+  +     E    F +++++DGCFVL+L   +      +G   
Sbjct: 172 YTNAMRELEEKARACYEGPISLSRNE----FTEMLVLDGCFVLELFRGTVEGFTEIGYAR 231

Query: 159 -------RCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNEN------------------ 218
                  R ++  I+RDM++LENQLPL +L +L  L    +N                  
Sbjct: 232 NDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLELQLGTQNQTGIVAHVAVKFFDPLMP 291

Query: 219 --EKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHENTITILE 278
             E      ++ + +W+  S + L  +  LH LD++R SLL      Q     NT ++L+
Sbjct: 292 TGEALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLL------QSSPTPNTRSLLK 351

Query: 279 WTLTNEENVEYCQSN-IRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDI 338
               N   V+  Q   +   T+LR+AG+KF K  T+   D+  +FK   L +P L I D 
Sbjct: 352 RLTRNTRVVDKRQQQLVHCVTELREAGVKFRKRKTDRFWDI--EFKNGYLEIPKLLIHD- 411

Query: 339 LTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGV 398
            T+    N++AFE+ H+     +TS+++ M NLI   +DVS L   GI+   +  D   V
Sbjct: 412 GTKSLFSNLIAFEQCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHWLGSDSE-V 471

Query: 399 VQLFNVLAKGQT-KYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQIISIA 424
             LFN L +       +SH+  L   +N Y++ +W++    L  K     N W   S +
Sbjct: 472 ADLFNRLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHK--YFNNPWAYFSFS 514

BLAST of Cucsat.G6325 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 156.8 bits (395), Expect = 4.2e-38
Identity = 135/463 (29.16%), Postives = 214/463 (46.22%), Query Frame = 0

Query: 16  VNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLL 75
           V ++  KLE++   ++  +T   K  IY++P +++E + K+Y P+ VSLGPYH+GK  L 
Sbjct: 81  VISITDKLEQAHRDDD--TTLWGKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLR 140

Query: 76  PMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIV 135
            M+R K +A      R N  ++  +D++  + E     Y+  L     E    F++++++
Sbjct: 141 SMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGPLSLSSNE----FIEMLVL 200

Query: 136 DGCFVLDLI--SELSSKSLG----------RCMIWDIKRDMVLLENQLPLQLLKQL---- 195
           DGCFVL+L   +      LG          R  +  I+RDMV+LENQLPL +L +L    
Sbjct: 201 DGCFVLELFRGAVEGFTELGYARNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLELQ 260

Query: 196 -------------------------HALTTTNENEKENLDLEAMIWSWMGLSGEILSMRS 255
                                      LT + +++ EN       +      GE      
Sbjct: 261 LGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSFDPFADMGE------ 320

Query: 256 PLHILDIYRSSLLSPTRCKQDETHENTITILEWTLTNEENVEYCQSNIRSATQLRKAGIK 315
            LH LD++R SLL     +     E  +T   W+       +  Q  I   T+L++AGIK
Sbjct: 321 -LHCLDVFRRSLL-----RSSPKPEPRLTRKRWSRNTRVADKRRQQLIHCVTELKEAGIK 380

Query: 316 FEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVL 375
           F +  T+   D+   FK   L +P L I D  T+   +N++AFE+ H+     +TS+++ 
Sbjct: 381 FRRRKTDRFWDM--QFKNGYLEIPRLLIHD-GTKSLFLNLIAFEQCHIDSSNDITSYIIF 440

Query: 376 MKNLIGMEKDVSLLASKGILSSNVIHDGNGVVQLFNVLAKGQTKYLE-SHMYELFRMLNC 435
           M NLI   +DVS L   GI+   +  D   V  LFN L +      E S++  L   +N 
Sbjct: 441 MDNLIDSHEDVSYLHYCGIIEHWLGSDSE-VADLFNRLCQEVVFDTEDSYLSRLSIEVNR 500

Query: 436 YHDNRWSIWLENLKLKVPQTKNSWQIISIALFTFGFTYPFVQA 437
           Y+D++W+ W   LK K     N W I+S           F Q+
Sbjct: 501 YYDHKWNAWRATLKHK--YFNNPWAIVSFCAAVILLVLTFSQS 519

BLAST of Cucsat.G6325 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-36
Identity = 132/460 (28.70%), Postives = 219/460 (47.61%), Query Frame = 0

Query: 16  VNALLSKLEESIGMEEFLSTQHIKPSIYKIPQFIREVNPKAYEPKLVSLGPYHYGKPHLL 75
           V ++  K+E++  + E  +T   K  IY++PQ+++E N K+Y P+ VSLGP+H+G  HLL
Sbjct: 117 VISIRDKMEQA--LREDATTSWDKLCIYRVPQYLQENNKKSYFPQTVSLGPFHHGNKHLL 176

Query: 76  PMEREKHKAFLHFKARNNLYLESIVDSVCSILEDLLGSYDDNLDDRWKEDVAMFLKLMIV 135
           PM+R K +A     AR    +E  +D++  + +     Y+  +D    +    F +++++
Sbjct: 177 PMDRHKWRAVNMVMARTKHDIEMYIDAMKELEDRARACYEGPIDLSSNK----FSEMLVL 236

Query: 136 DGCFVLDLI--SELSSKSLG----------RCMIWDIKRDMVLLENQLPLQLLKQLHALT 195
           DGCFVL+L   ++     LG          R  +  I+RDMV+LENQLPL +L +L  + 
Sbjct: 237 DGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEIQ 296

Query: 196 TTNENEKENLD-LEAMIWSWMGLSGEILSM------------------RSPLHILDIYRS 255
               ++   +  L    +  +  + E L+                   +  LH LD++R 
Sbjct: 297 LGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIADKDKGELHCLDVFRR 356

Query: 256 SLLSPTRCKQDETHENTITILEWTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLM 315
           +LL P  C   E     ++ + W+       +  Q  I   T+LR+AGIKF    T+   
Sbjct: 357 NLLRP--CSNPEPR---LSRMRWSWRTRVADKRQQQLIHCVTELREAGIKFRTRKTDRFW 416

Query: 316 DVSFDFKQRVLRLPSLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKD 375
           D+   FK   L +P L I D  T+    N++AFE+ H+     +TS+++ M NLI   +D
Sbjct: 417 DIR--FKNGYLEIPKLLIHD-GTKSLFSNLIAFEQCHIDSSNDITSYIIFMDNLIDSSED 476

Query: 376 VSLLASKGILSSNVIHDGNGVVQLFNVLAKGQT-KYLESHMYELFRMLNCYHDNRWSIWL 434
           V  L   GI+   + +D   V  LFN L +        S++ +L   ++  +  +W++  
Sbjct: 477 VRYLHYCGIIEHWLGNDYE-VADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYSRKWNVLK 536