Cucsat.G5941 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G5941
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	flavonol synthase/flavanone 3-hydroxylase
Location	ctg1402: 2895462 .. 2897342 (-)
RNA-Seq Expression	Cucsat.G5941
Synteny	Cucsat.G5941

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAAACTTTTTTAGAAAAAGGAAGGGTTAGTAATATAGAAAAGTAGAAAATGTTGAATCCAAATATAAATTTTAATTATTAAGAGGTGATTGTCCCAAACACAGGCATTAGTATTTTTAAAATTATGTATGTTTAGGCATCACTCTTTTGCTCACCAATATTCATAAGAGGCGCATGCTGAGTAGGAACGGAAGAGGGTTGCAGCGCTTGAATTCTGTTGTGAAAACACGCACAAAAGTGGAAGTTTGTTAAACCGTATCTACTGAAAGCAACCTTCTACCTTCTCGACTCTCTTCAATTTTCATTGTATTAATTGAGCTTCTAAATTCCTTCTCTCCTCACCGAAAACAAAAATGGAGGTTGGGAGAGTCCAAGAAGTTGCTTCCTCTCTTCTTTTCTCCGGCACTCTCCCCTCCCAGTTTGTCCGTCCGGACACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATCGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCACTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTGGGGAAGGAATTCTTCCAGCTCCCACCTGAGATGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTAGAAGGCTACGGAACTAATCTCCAAAAAGAAATCGATGGAAAAAGAGGTTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGTAACGAAATCAATAAGTTCCTCCGAGTTCTTTTCTTAATAATTGGTTATTGTACTCAGTCTTGTTTTTGGGTGCACGTGTGTTCGAGAATATAGGGCAGCGAATGAAGAGTACAGAAAGCATTTACATGGGGTTGTTGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACAGACATGTCAGCCATTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGCCATTGGTATGATGTTAATTACATTCCTAACGCCCTTGTCATCCATATCGGAGATCAAATTGAGGTAAAGTGTTTCTTGAATTCAAGAGATGTTTGAAATTTTGAGTTGCGTCGGAAATGGCTGAATTAAAAGTTTGTTGTGTTGTGGGGGCAGATTCTGAGTAATGGAAAGTACAAGAGTGTGCTTCATAGGACGACTGTGAACAAGGAGAAAACGAGGATATCGTGGCCGGTGTTCTTGGAGCCACCGCCGGAGTTTGAGGTTGGGCCTCATCCGAAATTAGTCGATGAGAAAAATCCGGCAAGATATAAAACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAACTCAAAACACCGCCGCGGCTTCCTTTTATGGTCATCGTTTTATCTGCTCCGGCCACCGTGTTCTCTTTTATGCTTTCAATGTCTTAAATTATGCAATAAGGCTAATGGTAATTGTTTTTTTTTTTCAAAGTTTAAATTTAAATAATAACGAAACTTCCCTTGTTTAGTTGGAACTTATTTTGAGAATATAAAACCCTAAAATAACGGGTTAATTTACATTATAAAACGCCAAAATATTGACCCATCTCTATAACAAATTAAACTCATGCCATCGTTGCTTTCTTATAAACTTTATAGTTGGTTTTTAATTTTCTGTGACGAGAATTGAATTTATG

Coding sequence (CDS)

ATGGAGGTTGGGAGAGTCCAAGAAGTTGCTTCCTCTCTTCTTTTCTCCGGCACTCTCCCCTCCCAGTTTGTCCGTCCGGACACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATCGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCACTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTGGGGAAGGAATTCTTCCAGCTCCCACCTGAGATGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTAGAAGGCTACGGAACTAATCTCCAAAAAGAAATCGATGGAAAAAGAGGTTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGCAGCGAATGAAGAGTACAGAAAGCATTTACATGGGGTTGTTGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACAGACATGTCAGCCATTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGCCATTGGTATGATGTTAATTACATTCCTAACGCCCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGCTTCATAGGACGACTGTGAACAAGGAGAAAACGAGGATATCGTGGCCGGTGTTCTTGGAGCCACCGCCGGAGTTTGAGGTTGGGCCTCATCCGAAATTAGTCGATGAGAAAAATCCGGCAAGATATAAAACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Protein sequence

MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ

Homology

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BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 1.9e-144
Identity = 233/336 (69.35%), Postives = 278/336 (82.74%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ +AS      T+PS+++R + EQP  TT+ G VL VPVID  +PD++ ++  I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQ-LPPEMKEIYAKPPASKSVEGYGTNL 120
            DAS  WG+FQ+INH IP+EAI  LQ VGKEFF+ +P E KE+ AK P S  +EGYGT+L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 QKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSL 180
           QKE++GK+GWVDHLFH IWPPS++NY +WP+NPPSYR ANEEY K +  VV+++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQ 240
           GLGLEGHE+ E  GGDE+VYLLKINYYPPCPRPDLALGV AHTDMS ITILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPE 300
            F+DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 336
            EVGP PKL+ E NP ++KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

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BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.3e-142
Identity = 232/323 (71.83%), Postives = 271/323 (83.90%), Query Frame = 0


Query: 17  GTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQ 76
           GT+P++F+R + EQPGITTV G+VL VP+IDF++PD++ ++  I +AS NWGM+QI+NH 
Sbjct: 15  GTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHD 74

Query: 77  IPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQKEID----GKRGWVDH 136
           IP+E I+ LQAVGKEFF+LP E KE YAKPP S S+EGYGT L KEI      K+GWVD+
Sbjct: 75  IPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDN 134

Query: 137 LFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERV 196
           LF+ IWPPS +NY FWP+NPPSYR ANEEY KHLH VVEKLFR LSLGLGLEG ELK+  
Sbjct: 135 LFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAA 194

Query: 197 GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYI 256
           GGD L YLLKINYYPPCPRPDLALGV AHTDMS +TILVPN+VQGLQA +DG WYDV YI
Sbjct: 195 GGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYI 254

Query: 257 PNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEK 316
           PNALVIHIGDQ+EI+SNGKY SVLHRTTVNK+KTRISWPVFLEPP +  VGPHP+LV+  
Sbjct: 255 PNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAV 314

Query: 317 NPARYKTKKYSEYAYCKLNKIPQ 336
           N  +YKTKKY +Y YCK+NK+PQ
Sbjct: 315 NQPKYKTKKYGDYVYCKINKLPQ 337

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BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 5.1e-142
Identity = 229/335 (68.36%), Postives = 277/335 (82.69%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQE+AS      T+P++++R + EQP I+TV G VL VPVID ++ D+  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            +AS  WG+FQ++NH IPNE I  LQ VGK FF+LP E KE+ AKP  S+S+EGYGT LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFH IWPPS+INY FWP+NPP+YR ANEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           L LE +  K+  GGD+LVYL+KINYYPPCPRPDLALGV AHTDMSAIT+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           ++DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 336
           EVGP PKLV+E+NPA++KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

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BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 3.1e-139
Identity = 225/337 (66.77%), Postives = 275/337 (81.60%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDF--TNPDDDSVLT 60
           MEV RVQ ++       T+PS+++R ++EQP +TT++G VL VPVID   +N  +++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTN 120
            I +AS  WG+FQ++NH IP++AI  LQ VGKEFF+LP + KE+ AKP   + VEGYGT 
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLS 180
           LQKE+ GK+GWVDHLFHIIWP S++NYNFWP NPP YR ANEEY   L GVV+KLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGL 240
           LG+GLE HELK+  GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMSAITILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPP 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFEVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 336
           E   GP  KL+ ++NPA++KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

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BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-136
Identity = 219/335 (65.37%), Postives = 270/335 (80.60%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ +AS    +GT+P++F+RP+ EQP  TT  G    +P ID  +P  D ++ +I
Sbjct: 1   MEVERVQAIASLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            +AS  WG+FQ+ NH IP++ I  LQAVGKEFF+LP E KE+Y++P  +K V+GYGT LQ
Sbjct: 61  AEASREWGIFQVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE++GK+ WVDHLFH +WPPSSINY FWP+NPPSYRA NEEY K++  VV+KLF  LSLG
Sbjct: 121 KEVEGKKSWVDHLFHRVWPPSSINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LG+EG  LKE  GGD++ Y+LKINYYPPCPRPDLALGV AHTD+SA+T+LVPNEV GLQ 
Sbjct: 181 LGVEGGVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D  W D  YIPNALVIHIGDQIEILSNGKYK+VLHRTTVNK+KTR+SWPVFLEPP + 
Sbjct: 241 FKDDRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADT 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 336
            VGP P+LVD++NP +YK KK+ +Y+YCKLNK+PQ
Sbjct: 301 VVGPLPQLVDDENPPKYKAKKFKDYSYCKLNKLPQ 335

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BLAST of Cucsat.G5941 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 695 bits (1793), Expect = 1.04e-252
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVDEK PARYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

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BLAST of Cucsat.G5941 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 676 bits (1743), Expect = 4.36e-245
Identity = 321/335 (95.82%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
            VGPHPKLVDE+NPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 645 bits (1663), Expect = 6.83e-233
Identity = 304/334 (91.02%), Postives = 320/334 (95.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS+L+S TLP++FVR +TEQPGITTVKGQVLAVPVIDFT+PDDDSVL AI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHIIWPPS+INYNFWPQNPPSYRAANEEY KHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKER GG+ELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDG WYDVNYIPNALVIHIGDQ+EILSNGKYKSV HRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIP 334
           EVGPHPKLVDEKNP +YKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

[Close]

BLAST of Cucsat.G5941 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 627 bits (1616), Expect = 9.95e-226
Identity = 292/335 (87.16%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD+ NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627 bits (1616), Expect = 9.95e-226
Identity = 292/335 (87.16%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD+ NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 695 bits (1793), Expect = 5.03e-253
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVDEK PARYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 2.11e-245
Identity = 321/335 (95.82%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
            VGPHPKLVDE+NPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 2.11e-245
Identity = 321/335 (95.82%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
            VGPHPKLVDE+NPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 1.38e-225
Identity = 291/335 (86.87%), Postives = 314/335 (93.73%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DG++GWVDHLFHIIWPPS+IN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD+ NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 9.31e-224
Identity = 288/335 (85.97%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG++P++FVRPD EQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELK+  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSVLHRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD+ NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Cucsat.G5941 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 438.7 bits (1127), Expect = 4.1e-123
Identity = 198/334 (59.28%), Postives = 253/334 (75.75%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ+++SS L +  +P +F+R + EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FF+LP   KE  AKP  SK +EGYGT LQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIP 335
            VGP P+L  + NP ++K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Cucsat.G5941 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 438.7 bits (1127), Expect = 4.1e-123
Identity = 198/334 (59.28%), Postives = 253/334 (75.75%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ+++SS L +  +P +F+R + EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FF+LP   KE  AKP  SK +EGYGT LQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+VLHRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEKNPARYKTKKYSEYAYCKLNKIP 335
            VGP P+L  + NP ++K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Cucsat.G5941 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 332.0 bits (850), Expect = 5.4e-91
Identity = 151/294 (51.36%), Postives = 200/294 (68.03%), Query Frame = 0


Query: 41  LAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMK 100
           L +PVID +NPD++ V +A+  AS  WG+FQ++NH IP E I  L  VG EFF+LP   K
Sbjct: 12  LDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETEK 71

Query: 101 EIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANE 160
           E  AKP  S  +EGY T  QK+++G+  WVDHLFH IWPPS +N+ FWP+NPP Y   NE
Sbjct: 72  EAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNE 131

Query: 161 EYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPA 220
           EY  H+  + EK+   LS GLGL    LKE +GG+ + YL+KINYYPPCP P+L +G P 
Sbjct: 132 EYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAPD 191

Query: 221 HTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTT 280
           HTD++ IT+LV NE  GLQAF+D  W D  Y  + +++ IGDQ   +SNGKYKSV HR  
Sbjct: 192 HTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRAK 251

Query: 281 VNKEKTRISWPVFLEPPPEFEVGPHPKLV-DEKNPARYKTKKYSEYAYCKLNKI 334
           ++KEKTRISWPVF+E   +   GP P+L+  ++N  ++K   Y +Y + KL K+
Sbjct: 252 MDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

[Close]

BLAST of Cucsat.G5941 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 131/292 (44.86%), Postives = 190/292 (65.07%), Query Frame = 0


Query: 34  TTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFF 93
           +T+ G  + VPV+D +  D+D ++  +  AS  WG+FQ++NH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  QLPPEMKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPP 153
           +LP   KE  AK    +  EGY  N    + G   W +HLFH + PPS INY +WP+NPP
Sbjct: 84  ELPDAEKETVAK---EEDFEGYKKNY---LGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH+  + EK+   LS GLGL+     + +GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYK 273
           L +G  AH+DM AI +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+  ++NG+ K
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMRMTNGRLK 263

Query: 274 SVLHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEKNPARYKTKKYSEY 326
           +VLHR   +K+K RISWPVF+ P  +  VGP P+   ++NP +++T  Y++Y
Sbjct: 264 NVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 309

[Close]

BLAST of Cucsat.G5941 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-76
Identity = 131/293 (44.71%), Postives = 191/293 (65.19%), Query Frame = 0


Query: 34  TTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFF 93
           +T+ G  + VPV+D +  D+D ++  +  AS  WG+FQ++NH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  QLPPEMKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPP 153
           +LP   KE  AK    +  EGY  N    + G   W +HLFH + PPS INY +WP+NPP
Sbjct: 84  ELPDAEKETVAK---EEDFEGYKKNY---LGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH+  + EK+   LS GLGL+     + +GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEI-LSNGKY 273
           L +G  AH+DM AI +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+ + ++NG+ 
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMVWMTNGRL 263

Query: 274 KSVLHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEKNPARYKTKKYSEY 326
           K+VLHR   +K+K RISWPVF+ P  +  VGP P+   ++NP +++T  Y++Y
Sbjct: 264 KNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 310

The following BLAST results are available for this feature:

BLAST of Cucsat.G5941 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q07512	1.9e-144	69.35	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9XHG2	2.3e-142	71.83	Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2...	[more]
Q9M547	5.1e-142	68.36	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q7XZQ6	3.1e-139	66.77	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]
Q9ZWQ9	1.1e-136	65.37	Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ...	[more]

BLAST of Cucsat.G5941 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140862.1	1.04e-252	99.70	flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet...	[more]
XP_008445671.1	4.36e-245	95.82	PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921....	[more]
XP_038885635.1	6.83e-233	91.02	flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida]	[more]
KAG7016505.1	9.95e-226	87.16	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]
XP_023551148.1	9.95e-226	87.16	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G5941 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K885	5.03e-253	99.70	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A5D3CHN5	2.11e-245	95.82	Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BCS7	2.11e-245	95.82	flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A6J1FDW3	1.38e-225	86.87	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JY72	9.31e-224	85.97	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Cucsat.G5941 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08640.1	4.1e-123	59.28	flavonol synthase 1	[more]
AT5G08640.2	4.1e-123	59.28	flavonol synthase 1	[more]
AT5G63590.1	5.4e-91	51.36	flavonol synthase 3	[more]
AT5G63600.1	2.0e-77	44.86	flavonol synthase 5	[more]
AT5G63600.2	1.0e-76	44.71	flavonol synthase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..156 e-value: 5.6E-31 score: 109.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 43..150 e-value: 1.8E-22 score: 80.3
None	No IPR available	PANTHER	PTHR47990:SF42	FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASE	coord: 15..155
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 15..155
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 17..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G5941.T1	Cucsat.G5941.T1	mRNA
Cucsat.G5941.T3	Cucsat.G5941.T3	mRNA
Cucsat.G5941.T2	Cucsat.G5941.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0046872	metal ion binding