Cucsat.G581 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G581
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectinesterase
Location	ctg1: 11195306 .. 11198211 (-)
RNA-Seq Expression	Cucsat.G581
Synteny	Cucsat.G581

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAAAAATGTAAAAATTAAAATTAGTTGAACGAGACGTAAACTAATATATATATATAACAATTGGGTTTGAGCCCTCCAAATGAGAGAATAATAACGACCAACAATTTCCCGCTTTCTTTCTCTAACCTTCCCTTTCTCAAAACGAACCTTTTCCCCACAAATTCTATTTTCGATCAAATTCATTCTCAGAAAAGAAAATTTCAACTAATTTTCGCTTCCCTTAAATCCCATTAATCTCTCCTTCCAAATTCAAGTTCGTTTCGTTTCAGTCACCAATAATCCATCCCTTCCGTCTCTAAATTCATGGCTGATTCATCCTCGAATTCCCTATTCATCAAACCGATTTACATCGCATTTGCGATCGCTTTTACTTTCTTAATTTCATCTCCGATCGGTTCTTATGCCTTTACTGCACAAAATGTGATTGATTCGCCGTTGTTGACGAAGAAGATTGGTACAAATCGAACCATTAAGGTCGATATCAATGGAAATGGCGAATTCAAGTCCATTCAAGCTGCAGTCGATTCTGTTCCGGAAGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTACAGGTGATTTCTATGGTTTTCTTTTGCAGCTTTATTTTAGGATGTTGATTTGATGAAATGGTTCATTTTTGTGGTCTGAATTTACGAAAACGAGTGCGTCCTTATTTTTAATTTGGCTCTGTAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGGAATGGAAAAGGAAGGACGTCAATCGTTTGGTCACAGAGCTCTAGTGATAACGTTGAATCTGCTACTTTTAAAGTCGAAGCTCACAATTTCATTGCCTTTGGAGTTAGCTTCAAGGTCCGAATAGTTCTCGGTTTTTTACAGTGATTCTCTCTCTCTCTCTCTCTCTCTCTCTTTCTGTTGCTTGAATTAGCTTCAAAATGTATTCTATTAATGCATTGGTTTCTATTTCTTTTGGAAAATTTTGCTATTACAGAATGAGGCTCCGACTGGTGTGGCTTACACATCGCAAAATCAATCGGTAGCGGCATTCGTTGCGGCAGATAAAATTGCATTTTACCACTGTGGATTCTATAGCACTCACAATACTCTGTTCGATTATAAAGGCAGACATTACTATGACAAATGCTACATTCAAGGCTCCATTGATTTCATCTTTGGCCGAGGCAAATCCGTATTCCATGTAAAAACTCTTTTCCTCCCCCTTCTCGACTATATAGTTTTTTATTTTTGAAATTAAGATTTTAAATATAAATTCCATTGATTTGCTTTCTTAGTATCAGAAAATTATCCACATATAAATGCTACTTTTATTAGTTTCTTAATCTTCATACCTATTTATGATGAAAATTCTAAGCCAAATTTGTAAAAGTAGTGGTACATTAAACAGGAAATGATGAGAAAACAAAATTATATGATAGTGTTGATTGTCTATTGATTGATTTGTTATTTTATGAATTAAACTTCCATTTATTTGCTATCCAAAGTTAATAGGTTCAATTATACATTTTACTTTGGTTAAAAAGGTTAGTAACGAAACATGAATGCAAGAAGGTAGACATTTGGAAAAGAATAAAAGAGATCTATTTACTTTAGATATATTACATTGAATGGAAAAGAAATTAACAAAAGAGAAAGAAAGAAATAAAGAAAGATCTAGTGGGTTAAGAAGAGGTCAAAAAAAATGGAGGACGGAGCACATGTTATAACAGGTAATTTGATTGTATATATAATTGAAAATATTAAATTTTTTCATAGATATATTCCATGAAAAAGACAAGCATTAAAAGCCATATGTTGTCATTATTATAATTGGGTGTGTTACTGTATGGACGCAGAATTGTGAGATGTTTGTGATTGATGACAAGAGATTGACGATCCGTGGTTCAATCACAGCTCAGAACAGAAAGAGTGCAAATGAGAACAGTGGATTTGTTTTCATAAAAGGAAAGGTTTACGGAGTTGGCGGTACGTATTTGGGTAGAGCAAAAGGTGCATTTTCCAGAGTCATTTTTGCTAAAACTTACTTCTCAATCTCTGTTGTTCCTGCTGGTTGGACCAATTGGAGCCATGTTGGCAGCACTGAGTAAGTGAAATGCACCTAAATATACTTTTATCTCAACTAGTTGAGGTACGTTGGATGGAGTTAGTAGTGTAGAACTTAAGAAATCCTCATTTTGTTATATATTACAGGAATCTATACCATGGAGAATATGATTGTTATGGACCAGGATCAGAATCAGGAAATCGTGCGCCATGGGCTAAACAGTTAACTAAAGAGGAAGCTACCCCATTCATGGAAGTTACTTTTATCGATGGCACTGATTGGCTTCCTGCCTGGCTATAAAAGAAACAACAACTACTAAAAAGCTAAGCTAATACTACACACACACTCCTTCATGCTAGTCCTCCTTCCCTTTTGAATTTCCATTACTCTACAGTCCTTTTTGGTTTAATTATATTGGTTTTGCTTTTTGCTTTTTGCTTTTTGCTATTTTTTGTCTGTGACAATATCTTCATGCAAACTGCATTTGTAAAGATATTCTTAATTAAAAGTATATTCGATATATCAATGAGATGAAATACTGAAGAATTTCCTTTCAATTTTCTCTCTTATTATGTAGAATTGAAGTACAAATAGTAGTGATAATAATAATAATCATATGGCCGTCATAAAAACTTGTAGTCTTTGCATGAGCATTTTCTCTGTTGGTCACATGCATTAGTTTTGTAATTTATAATTTAGGAGTGTACTATTTACTACTCCCTTTGATCATATCGTATGAAATATATTATGTAATCTCTGATTTTAGACTCTGCCACCACCAAAGCCTCCTCCTCCACCTGCTCCACCTCCAAAACCACCACCACCA

Coding sequence (CDS)

ATGGCTGATTCATCCTCGAATTCCCTATTCATCAAACCGATTTACATCGCATTTGCGATCGCTTTTACTTTCTTAATTTCATCTCCGATCGGTTCTTATGCCTTTACTGCACAAAATGTGATTGATTCGCCGTTGTTGACGAAGAAGATTGGTACAAATCGAACCATTAAGGTCGATATCAATGGAAATGGCGAATTCAAGTCCATTCAAGCTGCAGTCGATTCTGTTCCGGAAGGGAATTCGCAGTGGATGATCATCCATGTTAGGAAAGGAATTTACAGAGAGAAGGTTCATATTCCGTCGAGTAAGCCGTACATCTTCTTGAGAGGGAATGGAAAAGGAAGGACGTCAATCGTTTGGTCACAGAGCTCTAGTGATAACGTTGAATCTGCTACTTTTAAAGTCGAAGCTCACAATTTCATTGCCTTTGGAGTTAGCTTCAAGAATGAGGCTCCGACTGGTGTGGCTTACACATCGCAAAATCAATCGGTAGCGGCATTCGTTGCGGCAGATAAAATTGCATTTTACCACTGTGGATTCTATAGCACTCACAATACTCTGTTCGATTATAAAGGCAGACATTACTATGACAAATGCTACATTCAAGGCTCCATTGATTTCATCTTTGGCCGAGGCAAATCCGTATTCCATAATTGTGAGATGTTTGTGATTGATGACAAGAGATTGACGATCCGTGGTTCAATCACAGCTCAGAACAGAAAGAGTGCAAATGAGAACAGTGGATTTGTTTTCATAAAAGGAAAGGTTTACGGAGTTGGCGGTACGTATTTGGGTAGAGCAAAAGGTGCATTTTCCAGAGTCATTTTTGCTAAAACTTACTTCTCAATCTCTGTTGTTCCTGCTGGTTGGACCAATTGGAGCCATGTTGGCAGCACTGAGAATCTATACCATGGAGAATATGATTGTTATGGACCAGGATCAGAATCAGGAAATCGTGCGCCATGGGCTAAACAGTTAACTAAAGAGGAAGCTACCCCATTCATGGAAGTTACTTTTATCGATGGCACTGATTGGCTTCCTGCCTGGCTATAA

Protein sequence

MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL

Homology

[Close]

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot
Match: Q9LSP1 (Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.1e-130
Identity = 218/332 (65.66%), Postives = 268/332 (80.72%), Query Frame = 0


Query: 20  IAFTFLISSPIGSYAFTAQNV-IDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVPE 79
           +  T ++ S  GS A   Q    D+PLLT+KI TNR+I VDI G G++ S+Q A+D+VP 
Sbjct: 9   LVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVPV 68

Query: 80  GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEAH 139
           GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T I  SQSS DNV SATFKVEA+
Sbjct: 69  GNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEAN 128

Query: 140 NFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDK 199
           +F+AFG+S +N+AP G+A+TS+NQSVAAFVAADK+AFYHC FYS HNTLFD KGRHYY +
Sbjct: 129 HFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYHE 188

Query: 200 CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYG 259
           CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GSITA +R+SA E +G+VFI+GKVYG
Sbjct: 189 CYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVYG 248

Query: 260 VGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTENLYHGEYDCYGPGSESGN 319
           +   YLGRAKG +SRVIFAKTY S +VVP GWTNWS+ GST+NLYHGEY C+GPG+E   
Sbjct: 249 IDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQK 308

Query: 320 RAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 351
           R+ WAK LTK+E   F+ + FIDGT WLP WL
Sbjct: 309 RSDWAKDLTKQEVESFLSIDFIDGTSWLPVWL 340

[Close]

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 9.5e-67
Identity = 128/299 (42.81%), Postives = 188/299 (62.88%), Query Frame = 0


Query: 52  TNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 111
           T+  + VD++G G F ++Q+A+D VP+ +S   +I V  G YREKV +  +K  + ++G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 112 GKGRTSIVW---SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFV 171
           G   TSI W   ++S+ +  +S +F V A NF A+ +SFKN AP      +  Q+VA  +
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 172 AADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKR 231
             D+ AFY CGFY   +TL D KGRH++ +C+IQGSIDFIFG G+S++ +C +  I    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 232 LT-IRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVP 291
            + + GSITAQ R+S +E SGF F+  K+ G G   LGRA GA++ V+F+ TY S  + P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 292 AGWTNWSHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWL 347
            GW NW      + +  GE+ CYGPG++   R  + KQLT  EA+ F++V+FIDG +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.4e-64
Identity = 125/308 (40.58%), Postives = 180/308 (58.44%), Query Frame = 0


Query: 49  KIGTNRTIKVDINGN-GEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 108
           K+  + T+ V    N G+F  IQ A+DS+P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 109 LRGNGKGRTSIVWSQSSS---------DNVESATFKVEAHNFIAFGVSFKNEAPTGVAYT 168
           + G G  +T++ W  ++              SA+F V +  F+A  ++F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 169 SQNQSVAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHN 228
              Q+VA  V+AD  AF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 229 CEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAK 288
           C +  I DK     G++TAQ R S  E++GF F+K KV G G  YLGRA G FSRV+FA 
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 289 TYFSISVVPAGWTNWSHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVT 347
           TY    ++P GW NW        +++G+Y C G G+  G R  WA++LT EEA PF+ +T
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

[Close]

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 7.5e-64
Identity = 129/293 (44.03%), Postives = 173/293 (59.04%), Query Frame = 0


Query: 56  IKVDINGNGEFKSIQAAVDSVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGK 115
           I+VD +G G+F  IQ A++S+P    NSQ   I V+ GIYREKV IP+ KPYI L G   
Sbjct: 51  IRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQA 110

Query: 116 GRTSIVWSQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKI 175
             T ++WS    D +ES T  + A +F+   ++ +N+      + +  ++VA  VAADK 
Sbjct: 111 SNTFLIWS-DGEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKA 170

Query: 176 AFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRG 235
           AFY C   S  +TL D  G HY+  CYI+G+ DFI G   S++  C +  +        G
Sbjct: 171 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NG 230

Query: 236 SITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNW 295
           SITAQ R SA E SGF F+  K+ G G T+LGR  GA+SRV+FA ++FS  V P GW  W
Sbjct: 231 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 290

Query: 296 SHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWL 347
                   +Y+GEY CYGPG++   R  W+KQL+ EEAT F+   FI G DWL
Sbjct: 291 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWL 332

[Close]

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.3e-63
Identity = 120/304 (39.47%), Postives = 181/304 (59.54%), Query Frame = 0


Query: 53  NRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNG 112
           ++ I V +NG+ +F+S+Q AVDS+P+ N++ + I +  G YREKV +P++KPYI  +G G
Sbjct: 58  HKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAG 117

Query: 113 KGRTSIVWSQSSSD---------NVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQS 172
           +  T+I W   +SD           ++A+  V A+ F A  +SF N AP  +      Q+
Sbjct: 118 RDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQA 177

Query: 173 VAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFV 232
           VA  ++ DK  F  CGFY   +TL D  GRHY+ +CYI+GSIDFIFG G+S++ +CE+  
Sbjct: 178 VAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHS 237

Query: 233 IDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSI 292
           I  +     GSI A  R    E +GF F+  +V G G  Y+GRA G +SR+++A TYF  
Sbjct: 238 IASR----FGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDA 297

Query: 293 SVVPAGWTNWSHVGS-TENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDG 347
            V   GW +W H  + ++  + G Y+CYGPG+ +     WA+ L  E A PF+  +F++G
Sbjct: 298 LVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNG 357

[Close]

BLAST of Cucsat.G581 vs. NCBI nr
Match: XP_011651086.1 (probable pectinesterase 67 [Cucumis sativus] >KGN57171.1 hypothetical protein Csa_009846 [Cucumis sativus])

HSP 1 Score: 711 bits (1834), Expect = 1.89e-258
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL
Sbjct: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350

[Close]

BLAST of Cucsat.G581 vs. NCBI nr
Match: XP_008438961.1 (PREDICTED: probable pectinesterase 67 [Cucumis melo] >KAA0049551.1 putative pectinesterase 67 [Cucumis melo var. makuwa] >TYK16228.1 putative pectinesterase 67 [Cucumis melo var. makuwa])

HSP 1 Score: 667 bits (1722), Expect = 2.23e-241
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAF IAF FLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDNVESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSANENSGFVFIKGKVYGVG TYLGRAKGA SRV+FAKTY SI+VVPAGWTNWS+VGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC GPGSES +RAPWAKQLTKEE  P M+V+FIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

[Close]

BLAST of Cucsat.G581 vs. NCBI nr
Match: XP_038888955.1 (probable pectinesterase 67 [Benincasa hispida])

HSP 1 Score: 640 bits (1650), Expect = 2.02e-230
Identity = 314/350 (89.71%), Postives = 329/350 (94.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSS SLF  PI+IAF IAF  LI SPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSKSLF-NPIHIAFVIAFALLILSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFTSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDN+ESATFKVEA NFIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADKI+FYHCGF
Sbjct: 121 SQSSSDNIESATFKVEAQNFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKISFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYY  CYIQGSIDFIFGR +SVFHNCEMFV+DDKR TIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYHNCYIQGSIDFIFGRSRSVFHNCEMFVVDDKRSTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KS+NENSGFVFI+GKVYG+GG YLGRAKGAFSRVIFAKTYFS +V+PAGWTNWS+ GSTE
Sbjct: 241 KSSNENSGFVFIRGKVYGIGGVYLGRAKGAFSRVIFAKTYFSKTVLPAGWTNWSYDGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NL+H EY C+GPGS SG+RAPWAKQLTKEEA PFMEV FIDGTDWLPAWL
Sbjct: 301 NLFHAEYACHGPGSASGHRAPWAKQLTKEEAQPFMEVGFIDGTDWLPAWL 349

[Close]

BLAST of Cucsat.G581 vs. NCBI nr
Match: XP_022979848.1 (probable pectinesterase 67 [Cucurbita maxima])

HSP 1 Score: 633 bits (1633), Expect = 7.86e-228
Identity = 302/350 (86.29%), Postives = 331/350 (94.57%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL  KPI+IAFAIAF  LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRT IVW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSS+DN+ESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAAD +AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSA+ENSGFVFI+GKVYG+GG YLGRAKGAFSRV+FAKTYFS++V+PAGWTNWS+ G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC+GPG+E GNRAPWAKQLT++EA  FM+++FI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTEKEAAQFMDISFIEGADWLPAWL 349

[Close]

BLAST of Cucsat.G581 vs. NCBI nr
Match: KAG6582165.1 (putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 633 bits (1632), Expect = 1.12e-227
Identity = 303/350 (86.57%), Postives = 330/350 (94.29%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL   PI+IAFAIAF  LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSS+DN+ESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAF+AADK+AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSA+ENSGFVFI+GKVYG+GG YLGRAKGAFSRV+FAKTYFS++V+PAGWTNWS+ G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC+GPGSE GNRAPWAKQLT +EA  FM+++FI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGSEKGNRAPWAKQLTDKEADQFMDISFIEGADWLPAWL 349

[Close]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL
Match: A0A0A0LAR0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 9.13e-259
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL
Sbjct: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350

[Close]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL
Match: A0A5A7U7L5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001570 PE=4 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 1.08e-241
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAF IAF FLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDNVESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSANENSGFVFIKGKVYGVG TYLGRAKGA SRV+FAKTY SI+VVPAGWTNWS+VGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC GPGSES +RAPWAKQLTKEE  P M+V+FIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

[Close]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL
Match: A0A1S3AY88 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 1.08e-241
Identity = 328/350 (93.71%), Postives = 336/350 (96.00%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSLFIKPIYIAF IAF FLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI
Sbjct: 1   MADSSSNSLFIKPIYIAFVIAFAFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW
Sbjct: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSSSDNVESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAADK AFYHCGF
Sbjct: 121 SQSSSDNVESATFKVEAQHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADKAAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDSCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSANENSGFVFIKGKVYGVG TYLGRAKGA SRV+FAKTY SI+VVPAGWTNWS+VGSTE
Sbjct: 241 KSANENSGFVFIKGKVYGVGSTYLGRAKGAHSRVVFAKTYLSITVVPAGWTNWSYVGSTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC GPGSES +RAPWAKQLTKEE  P M+V+FIDGTDWLPAWL
Sbjct: 301 NLYHAEYDCRGPGSESKSRAPWAKQLTKEEVAPLMDVSFIDGTDWLPAWL 350

[Close]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL
Match: A0A6J1IXH1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1)

HSP 1 Score: 633 bits (1633), Expect = 3.80e-228
Identity = 302/350 (86.29%), Postives = 331/350 (94.57%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL  KPI+IAFAIAF  LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CKPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPY+FLRGNGKGRT IVW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYVFLRGNGKGRTHIVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSS+DN+ESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAFVAAD +AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFVAADNVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCE+FVIDDKRLT+RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEIFVIDDKRLTVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSA+ENSGFVFI+GKVYG+GG YLGRAKGAFSRV+FAKTYFS++V+PAGWTNWS+ G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYAGGTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC+GPG+E GNRAPWAKQLT++EA  FM+++FI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTEKEAAQFMDISFIEGADWLPAWL 349

[Close]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL
Match: A0A6J1GU67 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1)

HSP 1 Score: 630 bits (1625), Expect = 6.30e-227
Identity = 301/350 (86.00%), Postives = 330/350 (94.29%), Query Frame = 0


Query: 1   MADSSSNSLFIKPIYIAFAIAFTFLISSPIGSYAFTAQNVIDSPLLTKKIGTNRTIKVDI 60
           MADSSSNSL   PI+IAFAIAF  LISSPIGSY FTAQNVI+SPLLTKKI TNRTIKVDI
Sbjct: 1   MADSSSNSL-CNPIHIAFAIAFALLISSPIGSYGFTAQNVINSPLLTKKIATNRTIKVDI 60

Query: 61  NGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVW 120
           NGNGEF SIQAAVD+VPEGNSQWMIIHVRKGIYREKVH+PSSKPYIFLRGNGKGRT +VW
Sbjct: 61  NGNGEFTSIQAAVDAVPEGNSQWMIIHVRKGIYREKVHVPSSKPYIFLRGNGKGRTHMVW 120

Query: 121 SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGF 180
           SQSS+DN+ESATFKVEA +FIAFG+SFKNEAPTGVAYTSQNQSVAAF+AADK+AFYHCGF
Sbjct: 121 SQSSNDNIESATFKVEAKHFIAFGISFKNEAPTGVAYTSQNQSVAAFIAADKVAFYHCGF 180

Query: 181 YSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNR 240
           YSTHNTLFDYKGRHYYD CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRL++RGS+TAQNR
Sbjct: 181 YSTHNTLFDYKGRHYYDGCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLSVRGSVTAQNR 240

Query: 241 KSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTE 300
           KSA+ENSGFVFI+GKVYG+GG YLGRAKGAFSRV+FAKTYFS++V+PAGWTNWS+ G TE
Sbjct: 241 KSADENSGFVFIRGKVYGIGGVYLGRAKGAFSRVVFAKTYFSMTVLPAGWTNWSYSGGTE 300

Query: 301 NLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 350
           NLYH EYDC+GPG+E GNRAPWAKQLT +EA  FM+++FI+G DWLPAWL
Sbjct: 301 NLYHSEYDCHGPGAEKGNRAPWAKQLTDKEADQFMDISFIEGADWLPAWL 349

[Close]

BLAST of Cucsat.G581 vs. TAIR 10
Match: AT3G17060.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-131
Identity = 218/332 (65.66%), Postives = 268/332 (80.72%), Query Frame = 0


Query: 20  IAFTFLISSPIGSYAFTAQNV-IDSPLLTKKIGTNRTIKVDINGNGEFKSIQAAVDSVPE 79
           +  T ++ S  GS A   Q    D+PLLT+KI TNR+I VDI G G++ S+Q A+D+VP 
Sbjct: 9   LVLTLVVMSIWGSDASAMQKTKFDAPLLTEKIATNRSIIVDIEGKGDYTSVQKAIDAVPV 68

Query: 80  GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGKGRTSIVWSQSSSDNVESATFKVEAH 139
           GNS W+I+HVRKGIY+E+VHIP +KP+IF+RGNGKG+T I  SQSS DNV SATFKVEA+
Sbjct: 69  GNSNWIIVHVRKGIYKERVHIPENKPFIFMRGNGKGKTVIESSQSSVDNVASATFKVEAN 128

Query: 140 NFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDK 199
           +F+AFG+S +N+AP G+A+TS+NQSVAAFVAADK+AFYHC FYS HNTLFD KGRHYY +
Sbjct: 129 HFVAFGISIRNDAPVGMAFTSENQSVAAFVAADKVAFYHCAFYSLHNTLFDNKGRHYYHE 188

Query: 200 CYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYG 259
           CYIQGSIDFIFGR  S+F+NCE+FVI DKR+   GSITA +R+SA E +G+VFI+GKVYG
Sbjct: 189 CYIQGSIDFIFGRATSIFNNCEIFVISDKRVKPYGSITAHHRESAEEKTGYVFIRGKVYG 248

Query: 260 VGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNWSHVGSTENLYHGEYDCYGPGSESGN 319
           +   YLGRAKG +SRVIFAKTY S +VVP GWTNWS+ GST+NLYHGEY C+GPG+E   
Sbjct: 249 IDEVYLGRAKGPYSRVIFAKTYLSKTVVPDGWTNWSYHGSTQNLYHGEYKCHGPGAERQK 308

Query: 320 RAPWAKQLTKEEATPFMEVTFIDGTDWLPAWL 351
           R+ WAK LTK+E   F+ + FIDGT WLP WL
Sbjct: 309 RSDWAKDLTKQEVESFLSIDFIDGTSWLPVWL 340

[Close]

BLAST of Cucsat.G581 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 6.8e-68
Identity = 128/299 (42.81%), Postives = 188/299 (62.88%), Query Frame = 0


Query: 52  TNRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGN 111
           T+  + VD++G G F ++Q+A+D VP+ +S   +I V  G YREKV +  +K  + ++G 
Sbjct: 87  TSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGR 146

Query: 112 GKGRTSIVW---SQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFV 171
           G   TSI W   ++S+ +  +S +F V A NF A+ +SFKN AP      +  Q+VA  +
Sbjct: 147 GYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRI 206

Query: 172 AADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKR 231
             D+ AFY CGFY   +TL D KGRH++ +C+IQGSIDFIFG G+S++ +C +  I    
Sbjct: 207 EGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGN 266

Query: 232 LT-IRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVP 291
            + + GSITAQ R+S +E SGF F+  K+ G G   LGRA GA++ V+F+ TY S  + P
Sbjct: 267 TSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITP 326

Query: 292 AGWTNWSHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWL 347
            GW NW      + +  GE+ CYGPG++   R  + KQLT  EA+ F++V+FIDG +WL
Sbjct: 327 EGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of Cucsat.G581 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-65
Identity = 125/308 (40.58%), Postives = 180/308 (58.44%), Query Frame = 0


Query: 49  KIGTNRTIKVDINGN-GEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIF 108
           K+  + T+ V    N G+F  IQ A+DS+P  N   ++I V  G+Y+EKV IP  K +I 
Sbjct: 78  KLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFIT 137

Query: 109 LRGNGKGRTSIVWSQSSS---------DNVESATFKVEAHNFIAFGVSFKNEAPTGVAYT 168
           + G G  +T++ W  ++              SA+F V +  F+A  ++F+N  P  +   
Sbjct: 138 IEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGA 197

Query: 169 SQNQSVAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHN 228
              Q+VA  V+AD  AF+ C      +TL+D+ GRHYY  CYI+GS+DFIFG   S++  
Sbjct: 198 VGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEG 257

Query: 229 CEMFVIDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAK 288
           C +  I DK     G++TAQ R S  E++GF F+K KV G G  YLGRA G FSRV+FA 
Sbjct: 258 CHVHAIADK----LGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAY 317

Query: 289 TYFSISVVPAGWTNWSHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVT 347
           TY    ++P GW NW        +++G+Y C G G+  G R  WA++LT EEA PF+ +T
Sbjct: 318 TYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLT 377

[Close]

BLAST of Cucsat.G581 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 245.7 bits (626), Expect = 5.4e-65
Identity = 129/293 (44.03%), Postives = 173/293 (59.04%), Query Frame = 0


Query: 56  IKVDINGNGEFKSIQAAVDSVPE--GNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNGK 115
           I+VD +G G+F  IQ A++S+P    NSQ   I V+ GIYREKV IP+ KPYI L G   
Sbjct: 51  IRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQA 110

Query: 116 GRTSIVWSQSSSDNVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQSVAAFVAADKI 175
             T ++WS    D +ES T  + A +F+   ++ +N+      + +  ++VA  VAADK 
Sbjct: 111 SNTFLIWS-DGEDILESPTLTIFASDFVCRFLTIQNK------FGTAGRAVALRVAADKA 170

Query: 176 AFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFVIDDKRLTIRG 235
           AFY C   S  +TL D  G HY+  CYI+G+ DFI G   S++  C +  +        G
Sbjct: 171 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPN----NG 230

Query: 236 SITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSISVVPAGWTNW 295
           SITAQ R SA E SGF F+  K+ G G T+LGR  GA+SRV+FA ++FS  V P GW  W
Sbjct: 231 SITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQW 290

Query: 296 SHVGSTENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDGTDWL 347
                   +Y+GEY CYGPG++   R  W+KQL+ EEAT F+   FI G DWL
Sbjct: 291 GDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWL 332

[Close]

BLAST of Cucsat.G581 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 5.9e-64
Identity = 120/304 (39.47%), Postives = 181/304 (59.54%), Query Frame = 0


Query: 53  NRTIKVDINGNGEFKSIQAAVDSVPEGNSQWMIIHVRKGIYREKVHIPSSKPYIFLRGNG 112
           ++ I V +NG+ +F+S+Q AVDS+P+ N++ + I +  G YREKV +P++KPYI  +G G
Sbjct: 58  HKVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAG 117

Query: 113 KGRTSIVWSQSSSD---------NVESATFKVEAHNFIAFGVSFKNEAPTGVAYTSQNQS 172
           +  T+I W   +SD           ++A+  V A+ F A  +SF N AP  +      Q+
Sbjct: 118 RDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQA 177

Query: 173 VAAFVAADKIAFYHCGFYSTHNTLFDYKGRHYYDKCYIQGSIDFIFGRGKSVFHNCEMFV 232
           VA  ++ DK  F  CGFY   +TL D  GRHY+ +CYI+GSIDFIFG G+S++ +CE+  
Sbjct: 178 VAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHS 237

Query: 233 IDDKRLTIRGSITAQNRKSANENSGFVFIKGKVYGVGGTYLGRAKGAFSRVIFAKTYFSI 292
           I  +     GSI A  R    E +GF F+  +V G G  Y+GRA G +SR+++A TYF  
Sbjct: 238 IASR----FGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDA 297

Query: 293 SVVPAGWTNWSHVGS-TENLYHGEYDCYGPGSESGNRAPWAKQLTKEEATPFMEVTFIDG 347
            V   GW +W H  + ++  + G Y+CYGPG+ +     WA+ L  E A PF+  +F++G
Sbjct: 298 LVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNG 357

The following BLAST results are available for this feature:

BLAST of Cucsat.G581 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSP1	2.1e-130	65.66	Probable pectinesterase 67 OS=Arabidopsis thaliana OX=3702 GN=PME67 PE=2 SV=1	[more]
Q9ZQA3	9.5e-67	42.81	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q8VYZ3	3.4e-64	40.58	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9SIJ9	7.5e-64	44.03	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8LPF3	8.3e-63	39.47	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of Cucsat.G581 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651086.1	1.89e-258	100.00	probable pectinesterase 67 [Cucumis sativus] >KGN57171.1 hypothetical protein Cs...	[more]
XP_008438961.1	2.23e-241	93.71	PREDICTED: probable pectinesterase 67 [Cucumis melo] >KAA0049551.1 putative pect...	[more]
XP_038888955.1	2.02e-230	89.71	probable pectinesterase 67 [Benincasa hispida]	[more]
XP_022979848.1	7.86e-228	86.29	probable pectinesterase 67 [Cucurbita maxima]	[more]
KAG6582165.1	1.12e-227	86.57	putative pectinesterase 67, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G581 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAR0	9.13e-259	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G166360 PE=4 SV=1	[more]
A0A5A7U7L5	1.08e-241	93.71	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00157...	[more]
A0A1S3AY88	1.08e-241	93.71	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483896 PE=4 SV=1	[more]
A0A6J1IXH1	3.80e-228	86.29	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479420 PE=4 SV=1	[more]
A0A6J1GU67	6.30e-227	86.00	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457552 PE=4 SV=1	[more]

BLAST of Cucsat.G581 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17060.1	1.5e-131	65.66	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	6.8e-68	42.81	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	2.4e-65	40.58	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	5.4e-65	44.03	pectinesterase 11	[more]
AT5G47500.1	5.9e-64	39.47	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 13..298 e-value: 5.5E-63 score: 212.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 6..305 e-value: 5.3E-95 score: 320.5
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 4..305
None	No IPR available	PANTHER	PTHR31321:SF98	PECTINESTERASE 67-RELATED	coord: 4..305
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 157..166
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 7..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G581.T1	Cucsat.G581.T1	mRNA
Cucsat.G581.T5	Cucsat.G581.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity