Cucsat.G565 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G565
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Pectinesterase
Location	ctg1: 10932744 .. 10935069 (-)
RNA-Seq Expression	Cucsat.G565
Synteny	Cucsat.G565

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAAAGCCAGTTCTCTATCTTCCCTTTTTCCATTAACCAACATAAACCAAAAACCAAAAACAAAAAACACAAAAAAACAAACTGAAAAAGAAACCCCCCAAATTGCTAATCTAAAAGTAAAACAAAAACATGTCTGATCAAGGGAAGAACAAGAAAGCTGCTCTCATCGGTGTATCTTCCTTGTTTCTTGTTGCCATGGTAGTTGCAGTCGCAGTCGGTGTCAATCGAAATAGCCCCAACGCCAGCGGTGGTGGCGACAGTGCCGCCACAGCCGAGATTTCGACCTCCACCAAAGCAGTCAAGGCTCTCTGTCAGCCCACTGACTACCAAGAAACCTGCGAGAAAGCTCTGTCTGAGGCCGGAACCAACACGAGCGACCCACGTGAGCTAATCAAAGCTGGTTTCAATGTCGCCGTCAACGAAATCAAATGGGCCATTGGAAATTCCACCACTCTGAAAGAAGCCGCCTCTGACCCAATGGCTAAACAAGCTCTTGACGCCTGTGGTGAGCTTATGGATTATGCAATTGACGATCTCGTAATTTCCTTTCAAAGAATCACAGACAATTTCGACATGCAGAAACTCGATGATTACATCGAAGATCTTAAGGTTTGGTTGAGTGGCGCATTGACTTATCAAGAAACTTGCATAGACGGCTTCGAAAATGTCACCGGCGATACCGGGGAAAAGATGACGAAATTGTTGGAAACGTCGAAAGAATTGACAATTAATGGGCTTGGAATGGTGAGTGAAGTTACTTCCATATTGACCTCTTTTGGGCTCCCAGCAATTGGACGACGGTTAATGACGGAGGAATCTAATGAACAAAGGGAAGAACCGTCGTGGGTCAGAGACAGACGGGGGCTTCTCCAAGCCACCGGCGCCAACATCAAGGCGGATGCCGTGGTGGCGAAAGACGGAAGTGGAAAGTACAAAACTGTGACTGCAGCCTTAAATGATGTCCCAAAGAAGAGCAATAAAACGTTTGTGATTTACGTTAAGGCAGGGGTTTATCAAGAACAAGTAATGGTAGAGAAAAGTATGACTTGGGTGATGATGATCGGAGATGGTCCGACTAAAACTAAGATTACAGCCGGGAAGAACTACATCGATGGAACACCCACCTTCAAAACCGCTACCGTTTGTAAGTTCTAAGTCGCTATAATATATTAAATTTACTTCACTCCCGGCTTAAAATTATTAGTCATTACCTGATTTAACATAATAATAAGAAGAAGAAGAAAACAGATTAAGTTTTTAGGTTAATTTGTGATACAGCCGTGATTTTAATGTTTTGTATTCACAGCTGTGATTGGAAGCAACTTCATTGCGAAGGACATCGGCTTCGAGAACTCTGCCGGAGCAGCAAAGCATCAGGCGGTGGCACTTCGAGTTCAATCCGACATGTCAGTCTTCTACAATTGTCAAATGGACGGTTACCAAGACACTCTCTACACACACGCTCACCGTCAATTCTACCGAGACTGCACCATCACCGGAACCATCGATTTCATCTTCGGAAACGGCGCCGTTGTATTCCAAAACTGCAAGATCCTAGTGAGGAAGCCAATGGATAATCAACAATGCATCGTCACCGCGCAGGGCCGAACTCAACGGAAAGAGCCAACCGCCATAATCTTGCAAAATTGTGCCATTTCCTCAGCCCCTGACTTCTTCCCGATCCGCCACATCAACAAGGCTTTCCTGGGCCGGCCATGGAAGCAATATTCAAGAACAATCATAATGCAATCCCAAATTGATGACTTGATTCAACCTGAAGGGTGGCTTCCATGGACGGGAAATTTTGCTCTGAATACTCTGTTTTATGCAGAGATCAATAACAGGGGACCAGGAGCAGCTACGGATAAACGTGTGAAGTGGAAGGGAATTAAGAAGATCACTATGGAACACGCCTTAGATTTCACTGCTGCGAGATTTATTCGTGGAGATCCCTGGATCAAGCCCACCGGCGTTCCGTACACTTCGGGCATGATGGCAGTATAAAATTTTTATAAGAAAAAACAGAGGAAGAAACTATATAAAAAGCTACTCTTAGAGTACGGTGTAGAGTCTCCAAACATTCCAAATTTTGAATAAATAATTTCTTTTTTATTCCCTCCTTTATGAGATCTTATCATTTTAGTGCTTCAATTTTTTCACCTTTTTATGATTTTAATTGTTTAGCTTGATTGCCTTCCCCTTTTCAAGTGGGTATACATTCTAAGTTATTAATTTCATACTCTCTAGTCTCTTGTATCCAAAATAAACAAAAAACCATACTCTCTTCTCCACATAAAAAAAAACCATTGTTATATCATTCAAAC

Coding sequence (CDS)

ATGTCTGATCAAGGGAAGAACAAGAAAGCTGCTCTCATCGGTGTATCTTCCTTGTTTCTTGTTGCCATGGTAGTTGCAGTCGCAGTCGGTGTCAATCGAAATAGCCCCAACGCCAGCGGTGGTGGCGACAGTGCCGCCACAGCCGAGATTTCGACCTCCACCAAAGCAGTCAAGGCTCTCTGTCAGCCCACTGACTACCAAGAAACCTGCGAGAAAGCTCTGTCTGAGGCCGGAACCAACACGAGCGACCCACGTGAGCTAATCAAAGCTGGTTTCAATGTCGCCGTCAACGAAATCAAATGGGCCATTGGAAATTCCACCACTCTGAAAGAAGCCGCCTCTGACCCAATGGCTAAACAAGCTCTTGACGCCTGTGGTGAGCTTATGGATTATGCAATTGACGATCTCGTAATTTCCTTTCAAAGAATCACAGACAATTTCGACATGCAGAAACTCGATGATTACATCGAAGATCTTAAGGTTTGGTTGAGTGGCGCATTGACTTATCAAGAAACTTGCATAGACGGCTTCGAAAATGTCACCGGCGATACCGGGGAAAAGATGACGAAATTGTTGGAAACGTCGAAAGAATTGACAATTAATGGGCTTGGAATGGTGAGTGAAGTTACTTCCATATTGACCTCTTTTGGGCTCCCAGCAATTGGACGACGGTTAATGACGGAGGAATCTAATGAACAAAGGGAAGAACCGTCGTGGGTCAGAGACAGACGGGGGCTTCTCCAAGCCACCGGCGCCAACATCAAGGCGGATGCCGTGGTGGCGAAAGACGGAAGTGGAAAGTACAAAACTGTGACTGCAGCCTTAAATGATGTCCCAAAGAAGAGCAATAAAACGTTTGTGATTTACGTTAAGGCAGGGGTTTATCAAGAACAAGTAATGGTAGAGAAAAGTATGACTTGGGTGATGATGATCGGAGATGGTCCGACTAAAACTAAGATTACAGCCGGGAAGAACTACATCGATGGAACACCCACCTTCAAAACCGCTACCGTTTCTGTGATTGGAAGCAACTTCATTGCGAAGGACATCGGCTTCGAGAACTCTGCCGGAGCAGCAAAGCATCAGGCGGTGGCACTTCGAGTTCAATCCGACATGTCAGTCTTCTACAATTGTCAAATGGACGGTTACCAAGACACTCTCTACACACACGCTCACCGTCAATTCTACCGAGACTGCACCATCACCGGAACCATCGATTTCATCTTCGGAAACGGCGCCGTTGTATTCCAAAACTGCAAGATCCTAGTGAGGAAGCCAATGGATAATCAACAATGCATCGTCACCGCGCAGGGCCGAACTCAACGGAAAGAGCCAACCGCCATAATCTTGCAAAATTGTGCCATTTCCTCAGCCCCTGACTTCTTCCCGATCCGCCACATCAACAAGGCTTTCCTGGGCCGGCCATGGAAGCAATATTCAAGAACAATCATAATGCAATCCCAAATTGATGACTTGATTCAACCTGAAGGGTGGCTTCCATGGACGGGAAATTTTGCTCTGAATACTCTGTTTTATGCAGAGATCAATAACAGGGGACCAGGAGCAGCTACGGATAAACGTGTGAAGTGGAAGGGAATTAAGAAGATCACTATGGAACACGCCTTAGATTTCACTGCTGCGAGATTTATTCGTGGAGATCCCTGGATCAAGCCCACCGGCGTTCCGTACACTTCGGGCATGATGGCAGTATAA

Protein sequence

MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHALDFTAARFIRGDPWIKPTGVPYTSGMMAV

Homology

[Close]

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 604.0 bits (1556), Expect = 1.8e-171
Identity = 295/571 (51.66%), Postives = 404/571 (70.75%), Query Frame = 0


Query: 3   DQGKNKKA-ALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 62
           D+ K K+   +I +SS+ L++MVVAV VGV+ N  +    GDS   AE++ S KAVK +C
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD----GDSKGKAEVNASVKAVKDVC 66

Query: 63  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQA 122
            PTDY++TCE  L + G NT+DP EL+K  FNV + +I  A   S T+ E   D   + A
Sbjct: 67  APTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMA 126

Query: 123 LDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVT 182
           LD C ELMDYA+D+L  SF+ +   F+   LD+ + +L++WLS A++++ETC++GF+   
Sbjct: 127 LDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQ 186

Query: 183 GDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVR 242
           G+ GE M K L+T+ ELT NGL ++SE+++ +    +P +  R +  E       PSWV 
Sbjct: 187 GNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG-----FPSWVD 246

Query: 243 DR-RGLLQATGA--NIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQ 302
            R R LLQA  A  ++K D VVA+DGSG+YKT+  AL  VPKK N TFV+++KAG+Y+E 
Sbjct: 247 QRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEY 306

Query: 303 VMVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGA 362
           V V K+M+ ++ IGDGP KT I+  KNY DG  T++TATV+++G+ FIAK+IGFEN+AGA
Sbjct: 307 VQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGA 366

Query: 363 AKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQN 422
            KHQAVA+RVQSD S+F+NC+ DGYQDTLYTH+HRQF+RDCTI+GTIDF+FG+ A VFQN
Sbjct: 367 IKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQN 426

Query: 423 CKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQ 482
           C +LVRKP+ NQ C +TA GR   +E T  + Q C I+  PD+  ++  +KA+LGRPWK+
Sbjct: 427 CTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKE 486

Query: 483 YSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITM 542
           YSRTIIM + I D +QP+GW PW G+F L TLFY+E+ N GPG+A   RV W GIK ++ 
Sbjct: 487 YSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSE 546

Query: 543 EHALDFTAARFIRGDPWIKPTGVPYTSGMMA 570
           E  L FT A++I+GD WI   GVPYT+G++A
Sbjct: 547 EDILKFTPAQYIQGDDWIPGKGVPYTTGLLA 567

[Close]

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 9.7e-170
Identity = 292/569 (51.32%), Postives = 395/569 (69.42%), Query Frame = 0


Query: 3   DQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQ 62
           D  + K+  +I +SS+ L++MVVAV +GV+ N        D+A   EI+TS KA+K +C 
Sbjct: 9   DAKRKKRYVIISISSVLLISMVVAVTIGVSVNK------SDNAGDEEITTSVKAIKDVCA 68

Query: 63  PTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQAL 122
           PTDY+ETCE  L +   +TSDP EL+K  FN  + +I      S T+ E   DP AK AL
Sbjct: 69  PTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMAL 128

Query: 123 DACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTG 182
           D C ELMDYAI +L  SF+ +   F+  K+D+ +  L++WLS  +++++TC+DGF+   G
Sbjct: 129 DQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQG 188

Query: 183 DTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-GRRLMTEESNEQREEPSWVR 242
           + GE + K L+T+ +LT NGL MV+E+++ L    +P +  RRL+++E       PSW+ 
Sbjct: 189 NAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEF------PSWMD 248

Query: 243 DR-RGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 302
            R R LL A  + +K D VVA+DGSG+YKT+  ALN VPKK N TFV+++K G+Y+E V 
Sbjct: 249 ARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQ 308

Query: 303 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 362
           V +SMT ++ IGDGP KT I+  K+Y DG  T+KTATV+++G +FIAK+I FEN+AGA K
Sbjct: 309 VNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIK 368

Query: 363 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 422
           HQAVA+RV +D S+FYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+ A VFQNC 
Sbjct: 369 HQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCT 428

Query: 423 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 482
           +LVRKP+ NQ C +TA GR   +E T  +LQ C I   PD+  ++  +K +LGRPWK+YS
Sbjct: 429 LLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYS 488

Query: 483 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 542
           RTIIM + I D + PEGW PW G F LNTLFY+E+ N GPGAA  KRV W GIKK++ E 
Sbjct: 489 RTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEE 548

Query: 543 ALDFTAARFIRGDPWIKPTGVPYTSGMMA 570
            L FT A++I+GD WI   GVPY  G+ +
Sbjct: 549 ILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

[Close]

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.5e-165
Identity = 291/571 (50.96%), Postives = 397/571 (69.53%), Query Frame = 0


Query: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65
           K KK  + GV +  LV MVVAV +  +RN+ ++    +     +I T+T AV+A+C PTD
Sbjct: 8   KKKKCIIAGVITALLVLMVVAVGITTSRNTSHS----EKIVPVQIKTATTAVEAVCAPTD 67

Query: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTT--LKEAASDPMAKQALD 125
           Y+ETC  +L +A  +++ P +LIK GFNV +  I+ +I  ++     +AA+D   K AL+
Sbjct: 68  YKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALE 127

Query: 126 ACGELMDYAIDDLVISFQRITDNFD---MQKLDDYIEDLKVWLSGALTYQETCIDGFENV 185
            C +LM+ A DDL    ++  DNFD   + +++D++EDL+VWLSG++ YQ+TC+D FE  
Sbjct: 128 LCEKLMNDATDDL----KKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 187

Query: 186 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-------GRRLMTEESNEQ 245
                + M K+ +TS+ELT NGL M++ ++++L  F +  +        R+L++ E    
Sbjct: 188 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG-- 247

Query: 246 REEPSWVRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAG 305
              PSWV      L AT   +KA+ VVA DGSG+YKT+  ALN VPK + K FVIY+K G
Sbjct: 248 --IPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 307

Query: 306 VYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYIDG-TPTFKTATVSVIGSNFIAKDIGF 365
           VY E+V V K MT V  IGDGPTKTKIT   NY  G   T+ TATV++ G NF AK+IGF
Sbjct: 308 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 367

Query: 366 ENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNG 425
           EN+AG   HQAVALRV +D++VFYNCQ+DGYQDTLY H+HRQF+RDCT++GT+DFIFG+G
Sbjct: 368 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 427

Query: 426 AVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFL 485
            VV QNC I+VRKPM +Q C++TAQGR+ ++E T ++LQNC I+  P + P++ INKA+L
Sbjct: 428 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 487

Query: 486 GRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKG 545
           GRPWK++SRTIIM + IDD+I P GWLPW G+FALNTL+YAE  N GPG+   +RVKW G
Sbjct: 488 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 547

Query: 546 IKKITMEHALDFTAARFIRGDPWIKPTGVPY 564
           IKK++ + AL FT ARF+RG+ WI P  VPY
Sbjct: 548 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 516.2 bits (1328), Expect = 4.9e-145
Identity = 267/578 (46.19%), Postives = 387/578 (66.96%), Query Frame = 0


Query: 2   SDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 61
           SD  K KK  + G  S FLV MVV+VAV  +++SP             I  +TKAV+A+C
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDEN-------HIRKTTKAVQAVC 62

Query: 62  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASD--PMAK 121
            PTD+++TC  +L  A  ++ DP +LIK GF V +  I  ++  ++   +A +D  P AK
Sbjct: 63  APTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAK 122

Query: 122 QALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFEN 181
            A + C +LM  AIDDL    + +   F + +++ ++EDL+VWLSG++ +Q+TC+D F  
Sbjct: 123 GAFELCEKLMIDAIDDLK---KCMDHGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGE 182

Query: 182 VTGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-------GRRLMTEESNE 241
           +  +  + M K+ +TS+EL+ N L MV+ +++++ +  L  +        R+L++ E + 
Sbjct: 183 IKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDS- 242

Query: 242 QREEPSWV-RDRRGLLQATGAN---IKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVI 301
               P+WV  + R L+ A G     +KA+AVVA+DG+G++KT+T ALN VPK +   F+I
Sbjct: 243 ---IPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 302

Query: 302 YVKAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNY-IDGTPTFKTATVSVIGSNFIA 361
           ++K G+Y+E+V V K M  V  IGDGP KT IT   N+ I    TF TAT+++ G +F A
Sbjct: 303 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 362

Query: 362 KDIGFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDF 421
           K+IG EN+AG    QAVALRV +D +VF++CQ+DG+QDTLY H+HRQFYRDCT++GT+DF
Sbjct: 363 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 422

Query: 422 IFGNGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHI 481
           IFG+   + QNCKI+VRKP   Q C+VTAQGR+  +E T ++L  C I+  P + P++ +
Sbjct: 423 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 482

Query: 482 NKAFLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKR 541
           NKA+LGRPWK++SRTIIM++ IDD+I P GWLPW+G+FAL TL+YAE  N GPG+   +R
Sbjct: 483 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 542

Query: 542 VKWKGIKKITMEHALDFTAARFIRGDPWIKPTGVPYTS 566
           VKW GIKK+T + AL +T  RF+RGD WI  T VPYT+
Sbjct: 543 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-124
Identity = 267/704 (37.93%), Postives = 373/704 (52.98%), Query Frame = 0


Query: 9   KAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTDYQE 68
           K  L+GV++L ++AMV+ V    + NSP+         +  + T T  V  +C  TDY++
Sbjct: 3   KYVLLGVTAL-IMAMVICVEAN-DGNSPSRKMEEVHRESKLMITKT-TVSIICASTDYKQ 62

Query: 69  TCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAI--GNSTTLKEAASDPMAKQALDACG 128
            C  +L  A   + DPR LI++ F++A+  I+  I  G       A +D   ++AL+ C 
Sbjct: 63  DCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 129 ELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTGE 188
           ELMD AIDDL  +  +    F   +L D++EDL VWLSG++TYQ+TCIDGFE +  +   
Sbjct: 123 ELMDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 189 KMTKLLETSKELTINGLGMVSEVTSILTSFGLP------------AIGRRLMTEES---- 248
            M +++   + LT NGL + + +  +L +F +P             +G     +ES    
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 309 ------------------------------------------------------------ 368
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 369 -----NEQREEPSWV--RDRRGLLQATGAN-IKADAVVAKDGSGKYKTVTAALNDVPKKS 428
                +E+ E P WV    RR L +    N IKA+ VVAKDGSGK KT+  AL  VP K+
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKN 422

Query: 429 NKTFVIYVKAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYI-DGTPTFKTATVSVI 488
            K FVI++K GVY+E+V V K M  VM +GDGPTKT IT    ++ D   T++TA+V+V 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 489 GSNFIAKDIGFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTI 548
           G  F+AKDIGFEN+AGAA+HQAVALRV +D +VF+NC M+GYQDTLY H HRQFYR+C +
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 549 TGTIDFIFGNGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDF 566
           +GTIDF+FG+   VFQNC+ ++R+PM++QQCIVTAQGR  R+E T I++ N  I+    +
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

[Close]

BLAST of Cucsat.G565 vs. NCBI nr
Match: XP_004134493.2 (pectinesterase [Cucumis sativus] >KGN57132.1 hypothetical protein Csa_009573 [Cucumis sativus])

HSP 1 Score: 1135 bits (2935), Expect = 0.0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0


Query: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60
           MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL
Sbjct: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60

Query: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120
           CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ
Sbjct: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120

Query: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180
           ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV
Sbjct: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180

Query: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240
           TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV
Sbjct: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240

Query: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300
           RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM
Sbjct: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300

Query: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360
           VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK
Sbjct: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360

Query: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420
           HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK
Sbjct: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420

Query: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480
           ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS
Sbjct: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480

Query: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540
           RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH
Sbjct: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540

Query: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           ALDFTAARFIRGDPWIKPTGVPYTSGMMAV
Sbjct: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570

[Close]

BLAST of Cucsat.G565 vs. NCBI nr
Match: KAA0049514.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK16194.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 515/570 (90.35%), Postives = 540/570 (94.74%), Query Frame = 0


Query: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60
           MSD GKNKKAALIGVSSLFLVAMVVAVAVGVNR +P     G   ATAEISTSTKAV+AL
Sbjct: 1   MSDGGKNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQAL 60

Query: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120
           CQPTDY+ETCEKALSEAGTNTSDPRELIK+GF+VAVNEIKWAIGNST LKEAASDPMAKQ
Sbjct: 61  CQPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQ 120

Query: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180
           ALDACGELMDYAIDDLVISF RITDNFD+QKL+DY+EDLK+WLSGALTYQETCIDGFENV
Sbjct: 121 ALDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENV 180

Query: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240
           TGDTGEKM KLL+TSKELT NGL MVSEV SI+TSFGLPA+GRRL+TEESN+Q EEPSWV
Sbjct: 181 TGDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSFGLPAVGRRLLTEESNQQEEEPSWV 240

Query: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300
            DRRGLLQATGA +KAD VVAKDGSGKYKTVT ALNDVPKKSNKTFVI+VKAGVYQEQVM
Sbjct: 241 GDRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGVYQEQVM 300

Query: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360
           V+KSMTWVMMIGDGPTKTKITAGKNY+DGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK
Sbjct: 301 VDKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360

Query: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420
           HQAVALRVQSDMS+FYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN A VFQNCK
Sbjct: 361 HQAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAAVFQNCK 420

Query: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480
           ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISS P FFP+RHINKA+LGRPWKQYS
Sbjct: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGRPWKQYS 480

Query: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540
           RTIIMQ QIDDLIQPEGWLPW G+FAL+TLFYAEINNRGPGAATDKRVKWKGIKKIT +H
Sbjct: 481 RTIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIKKITPQH 540

Query: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           ALDFT  RFIRGDPWIKPTGVPY+SGMMAV
Sbjct: 541 ALDFTVGRFIRGDPWIKPTGVPYSSGMMAV 570

[Close]

BLAST of Cucsat.G565 vs. NCBI nr
Match: XP_008438906.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1039 bits (2687), Expect = 0.0
Identity = 513/569 (90.16%), Postives = 539/569 (94.73%), Query Frame = 0


Query: 2   SDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 61
           S+ GKNKKAALIGVSSLFLVAMVVAVAVGVNR +P     G   ATAEISTSTKAV+ALC
Sbjct: 11  SNPGKNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQALC 70

Query: 62  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQA 121
           QPTDY+ETCEKALSEAGTNTSDPRELIK+GF+VAVNEIKWAIGNST LKEAASDPMAKQA
Sbjct: 71  QPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQA 130

Query: 122 LDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVT 181
           LDACGELMDYAIDDLVISF RITDNFD+QKL+DY+EDLK+WLSGALTYQETCIDGFENVT
Sbjct: 131 LDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENVT 190

Query: 182 GDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVR 241
           GDTGEKM KLL+TSKELT NGL MVSEV SI+TSFGLPA+GRRL+TEESN+Q EEPSWV 
Sbjct: 191 GDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSFGLPAVGRRLLTEESNQQEEEPSWVG 250

Query: 242 DRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMV 301
           DRRGLLQATGA +KAD VVAKDGSGKYKTVT ALNDVPKKSNKTFVI+VKAGVYQEQVMV
Sbjct: 251 DRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGVYQEQVMV 310

Query: 302 EKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH 361
           +KSMTWVMMIGDGPTKTKITAGKNY+DGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH
Sbjct: 311 DKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH 370

Query: 362 QAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKI 421
           QAVALRVQSDMS+FYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN A VFQNCKI
Sbjct: 371 QAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAAVFQNCKI 430

Query: 422 LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSR 481
           LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISS P FFP+RHINKA+LGRPWKQYSR
Sbjct: 431 LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGRPWKQYSR 490

Query: 482 TIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHA 541
           TIIMQ QIDDLIQPEGWLPW G+FAL+TLFYAEINNRGPGAATDKRVKWKGIKKIT +HA
Sbjct: 491 TIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIKKITPQHA 550

Query: 542 LDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           LDFT  RFIRGDPWIKPTGVPY+SGMMAV
Sbjct: 551 LDFTVGRFIRGDPWIKPTGVPYSSGMMAV 579

[Close]

BLAST of Cucsat.G565 vs. NCBI nr
Match: XP_038874833.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1011 bits (2614), Expect = 0.0
Identity = 500/568 (88.03%), Postives = 533/568 (93.84%), Query Frame = 0


Query: 3   DQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQ 62
           D GKNKKAALIGVSSLFLVAMVVAVAVGVNR   N   GG S  TAEISTSTKA+KALCQ
Sbjct: 4   DGGKNKKAALIGVSSLFLVAMVVAVAVGVNRKDSNGIAGGGS--TAEISTSTKAIKALCQ 63

Query: 63  PTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQAL 122
           PTDYQETCEKALS+ G+NTSDPRELIKAGF+ AV+EIK AIGNS+ LK++A+DPMAKQAL
Sbjct: 64  PTDYQETCEKALSQGGSNTSDPRELIKAGFHAAVDEIKSAIGNSSLLKDSATDPMAKQAL 123

Query: 123 DACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTG 182
           DACGELMDYAIDDL+ISF RITDNFD+QK+DDYIEDLKVWLSGALTYQETC+DGFENVTG
Sbjct: 124 DACGELMDYAIDDLLISFDRITDNFDIQKMDDYIEDLKVWLSGALTYQETCLDGFENVTG 183

Query: 183 DTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVRD 242
           DTGEKM  LL+ SKE+TINGLGMVSEV SI+ SFGLP+ GRRL+TEES+EQ EEPSWV D
Sbjct: 184 DTGEKMKNLLKMSKEMTINGLGMVSEVNSIMASFGLPSAGRRLLTEESDEQEEEPSWVSD 243

Query: 243 RRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMVE 302
           RRGLLQATGA IKAD VVAKDGSGKYKTV+ ALNDVPKKSNKTFVIYVKAGVYQEQVMV+
Sbjct: 244 RRGLLQATGATIKADVVVAKDGSGKYKTVSEALNDVPKKSNKTFVIYVKAGVYQEQVMVD 303

Query: 303 KSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKHQ 362
           KSMTWVMMIGDGPTKTKITAGKN+IDGTPTFKTATV+VIGSNFIAKD+GFENSAGAAKHQ
Sbjct: 304 KSMTWVMMIGDGPTKTKITAGKNFIDGTPTFKTATVAVIGSNFIAKDMGFENSAGAAKHQ 363

Query: 363 AVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKIL 422
           AVALRVQSDMS+FYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKIL
Sbjct: 364 AVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKIL 423

Query: 423 VRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSRT 482
           VRKPM+NQQCIVTAQGRTQRKEPTAIILQNC ISS PDFFP+RHI+KA+LGRPWKQYSRT
Sbjct: 424 VRKPMENQQCIVTAQGRTQRKEPTAIILQNCVISSDPDFFPVRHIHKAYLGRPWKQYSRT 483

Query: 483 IIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHAL 542
           IIMQSQIDDLIQPEGWLPW G+FALNTLFY E NNRGPGAA D RVKW+GIK+IT EHA+
Sbjct: 484 IIMQSQIDDLIQPEGWLPWMGDFALNTLFYTEYNNRGPGAAKDNRVKWRGIKQITPEHAV 543

Query: 543 DFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           DFTA RFIRGDPWIKPTGVPYTSGMMAV
Sbjct: 544 DFTAGRFIRGDPWIKPTGVPYTSGMMAV 569

[Close]

BLAST of Cucsat.G565 vs. NCBI nr
Match: XP_023529046.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 435/572 (76.05%), Postives = 494/572 (86.36%), Query Frame = 0


Query: 3   DQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNA----SGGGDSAATAEISTSTKAVK 62
           D GK KKAALIGVSSLFLVAMVVAVAVGVNRN P+     SGGG    T EISTS KA+K
Sbjct: 6   DGGKKKKAALIGVSSLFLVAMVVAVAVGVNRNDPDEISRISGGGG---TTEISTSVKAIK 65

Query: 63  ALCQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMA 122
           ALC PTDYQETC ++LS  G NTSDPRELIK GF   +NE+K+AI  S TLKE ASDP A
Sbjct: 66  ALCHPTDYQETCIESLS--GVNTSDPRELIKVGFQSTINELKYAIAKSATLKEFASDPKA 125

Query: 123 KQALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFE 182
           K ALD C E+++YAIDDL++SF RITDNF++ K+ DYIEDLKVWLS  LT QETC+D F+
Sbjct: 126 KPALDVCNEVVEYAIDDLIMSFDRITDNFNINKITDYIEDLKVWLSATLTLQETCVDAFD 185

Query: 183 NVTGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPS 242
           NVTGDTG+KM +LL+TSKE+T NGLGMVSEVTSI++SFG+   GRRL+ EESN++ EEPS
Sbjct: 186 NVTGDTGDKMRQLLKTSKEMTANGLGMVSEVTSIMSSFGMRMTGRRLLEEESNKEEEEPS 245

Query: 243 WVRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQ 302
           W+ DRRGLLQAT   I+ + VVAKDGSGKYKTV  AL D+PKKSNKTFVIYVK GVY+EQ
Sbjct: 246 WINDRRGLLQATPKTIQPNVVVAKDGSGKYKTVNEALFDIPKKSNKTFVIYVKEGVYEEQ 305

Query: 303 VMVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGA 362
           VM+ KSMTWVMMIGDGP KTKIT+ KN++DGTPTFKTATV+V+G+NFIAKD+ FENSAG 
Sbjct: 306 VMMNKSMTWVMMIGDGPNKTKITSNKNFVDGTPTFKTATVAVVGTNFIAKDMWFENSAGP 365

Query: 363 AKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQN 422
            KHQAVALRVQSDMS+FYNCQMDGYQDTLYTH HRQFYRDCTITGTIDFIFGN AVVFQN
Sbjct: 366 EKHQAVALRVQSDMSIFYNCQMDGYQDTLYTHTHRQFYRDCTITGTIDFIFGNAAVVFQN 425

Query: 423 CKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQ 482
           CKI VRKP+ NQQCIVTAQGR+QRKEPTAIILQNC ISSAPD+FP+RH  KA+LGRPWK+
Sbjct: 426 CKIFVRKPLPNQQCIVTAQGRSQRKEPTAIILQNCLISSAPDYFPVRHTYKAYLGRPWKE 485

Query: 483 YSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITM 542
           YSRTII+Q +IDDLIQPEGWLPWTG+FALNTLFY+E N RG G+  DKRVKW+GIK+IT 
Sbjct: 486 YSRTIILQCKIDDLIQPEGWLPWTGDFALNTLFYSEYNCRGAGSKKDKRVKWRGIKEITP 545

Query: 543 EHALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           EHA+DFTA  FIRG+PWIKPTG+PY+SGMM +
Sbjct: 546 EHAIDFTAGLFIRGNPWIKPTGIPYSSGMMTL 572

[Close]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL
Match: A0A0A0L930 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G164510 PE=3 SV=1)

HSP 1 Score: 1135 bits (2935), Expect = 0.0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0


Query: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60
           MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL
Sbjct: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60

Query: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120
           CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ
Sbjct: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120

Query: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180
           ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV
Sbjct: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180

Query: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240
           TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV
Sbjct: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240

Query: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300
           RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM
Sbjct: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300

Query: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360
           VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK
Sbjct: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360

Query: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420
           HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK
Sbjct: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420

Query: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480
           ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS
Sbjct: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480

Query: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540
           RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH
Sbjct: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540

Query: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           ALDFTAARFIRGDPWIKPTGVPYTSGMMAV
Sbjct: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570

[Close]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL
Match: A0A5A7U2Q1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001220 PE=3 SV=1)

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 515/570 (90.35%), Postives = 540/570 (94.74%), Query Frame = 0


Query: 1   MSDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKAL 60
           MSD GKNKKAALIGVSSLFLVAMVVAVAVGVNR +P     G   ATAEISTSTKAV+AL
Sbjct: 1   MSDGGKNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQAL 60

Query: 61  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 120
           CQPTDY+ETCEKALSEAGTNTSDPRELIK+GF+VAVNEIKWAIGNST LKEAASDPMAKQ
Sbjct: 61  CQPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQ 120

Query: 121 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 180
           ALDACGELMDYAIDDLVISF RITDNFD+QKL+DY+EDLK+WLSGALTYQETCIDGFENV
Sbjct: 121 ALDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENV 180

Query: 181 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 240
           TGDTGEKM KLL+TSKELT NGL MVSEV SI+TSFGLPA+GRRL+TEESN+Q EEPSWV
Sbjct: 181 TGDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSFGLPAVGRRLLTEESNQQEEEPSWV 240

Query: 241 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 300
            DRRGLLQATGA +KAD VVAKDGSGKYKTVT ALNDVPKKSNKTFVI+VKAGVYQEQVM
Sbjct: 241 GDRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGVYQEQVM 300

Query: 301 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360
           V+KSMTWVMMIGDGPTKTKITAGKNY+DGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK
Sbjct: 301 VDKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 360

Query: 361 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 420
           HQAVALRVQSDMS+FYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN A VFQNCK
Sbjct: 361 HQAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAAVFQNCK 420

Query: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 480
           ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISS P FFP+RHINKA+LGRPWKQYS
Sbjct: 421 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGRPWKQYS 480

Query: 481 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 540
           RTIIMQ QIDDLIQPEGWLPW G+FAL+TLFYAEINNRGPGAATDKRVKWKGIKKIT +H
Sbjct: 481 RTIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIKKITPQH 540

Query: 541 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           ALDFT  RFIRGDPWIKPTGVPY+SGMMAV
Sbjct: 541 ALDFTVGRFIRGDPWIKPTGVPYSSGMMAV 570

[Close]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL
Match: A0A1S3AY67 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483865 PE=3 SV=1)

HSP 1 Score: 1039 bits (2687), Expect = 0.0
Identity = 513/569 (90.16%), Postives = 539/569 (94.73%), Query Frame = 0


Query: 2   SDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 61
           S+ GKNKKAALIGVSSLFLVAMVVAVAVGVNR +P     G   ATAEISTSTKAV+ALC
Sbjct: 11  SNPGKNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQALC 70

Query: 62  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQA 121
           QPTDY+ETCEKALSEAGTNTSDPRELIK+GF+VAVNEIKWAIGNST LKEAASDPMAKQA
Sbjct: 71  QPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQA 130

Query: 122 LDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVT 181
           LDACGELMDYAIDDLVISF RITDNFD+QKL+DY+EDLK+WLSGALTYQETCIDGFENVT
Sbjct: 131 LDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENVT 190

Query: 182 GDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVR 241
           GDTGEKM KLL+TSKELT NGL MVSEV SI+TSFGLPA+GRRL+TEESN+Q EEPSWV 
Sbjct: 191 GDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSFGLPAVGRRLLTEESNQQEEEPSWVG 250

Query: 242 DRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVMV 301
           DRRGLLQATGA +KAD VVAKDGSGKYKTVT ALNDVPKKSNKTFVI+VKAGVYQEQVMV
Sbjct: 251 DRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGVYQEQVMV 310

Query: 302 EKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH 361
           +KSMTWVMMIGDGPTKTKITAGKNY+DGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH
Sbjct: 311 DKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAKH 370

Query: 362 QAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCKI 421
           QAVALRVQSDMS+FYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGN A VFQNCKI
Sbjct: 371 QAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAAVFQNCKI 430

Query: 422 LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYSR 481
           LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISS P FFP+RHINKA+LGRPWKQYSR
Sbjct: 431 LVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGRPWKQYSR 490

Query: 482 TIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEHA 541
           TIIMQ QIDDLIQPEGWLPW G+FAL+TLFYAEINNRGPGAATDKRVKWKGIKKIT +HA
Sbjct: 491 TIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIKKITPQHA 550

Query: 542 LDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           LDFT  RFIRGDPWIKPTGVPY+SGMMAV
Sbjct: 551 LDFTVGRFIRGDPWIKPTGVPYSSGMMAV 579

[Close]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL
Match: A0A6J1GYH5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457997 PE=3 SV=1)

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 435/570 (76.32%), Postives = 493/570 (86.49%), Query Frame = 0


Query: 3   DQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPN--ASGGGDSAATAEISTSTKAVKAL 62
           D GKNKKAALIGVSSLFLVAMVVAVAVGVNRN P+  +S  GD   T EISTS KA+KAL
Sbjct: 6   DGGKNKKAALIGVSSLFLVAMVVAVAVGVNRNDPDEISSTSGDGGTT-EISTSVKAIKAL 65

Query: 63  CQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQ 122
           CQPTDYQETC ++LS  G NTSDPRELIK GF   +NE+K AI  S TLKE ASDP AK 
Sbjct: 66  CQPTDYQETCIESLS--GVNTSDPRELIKVGFQSTINELKSAIVKSATLKEFASDPKAKP 125

Query: 123 ALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENV 182
           ALD C E+++YAIDDL++SF RITDNF++ K+ DY+EDLKVWLS  LT Q+TC+D F+NV
Sbjct: 126 ALDVCNEVVEYAIDDLIMSFDRITDNFNINKITDYVEDLKVWLSATLTLQDTCVDAFDNV 185

Query: 183 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWV 242
           TGDTG+KM +LL+TSKE+T NGLGMVSEVTSI++SFG+   GRRL+ EESN++ EEPSW+
Sbjct: 186 TGDTGDKMRQLLKTSKEMTANGLGMVSEVTSIMSSFGMRLAGRRLLEEESNKEEEEPSWI 245

Query: 243 RDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 302
            DRRGLLQAT   I+ + VVAKDGSGKYKTV  AL D+PKKSNKTFVIYVK GVY+EQVM
Sbjct: 246 NDRRGLLQATPKTIQPNVVVAKDGSGKYKTVNEALFDIPKKSNKTFVIYVKEGVYEEQVM 305

Query: 303 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 362
           + KSMTWVMMIGDGP KTKIT+ KN+ DGTPTFKTATV+V+G+NFIAKD+ FENSAG  K
Sbjct: 306 MNKSMTWVMMIGDGPNKTKITSNKNFADGTPTFKTATVAVVGTNFIAKDMWFENSAGPEK 365

Query: 363 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 422
           HQAVALRVQSDMS+FYNCQMDGYQDTLYTH HRQFYRDCTITGTIDFIFGN AVVFQNCK
Sbjct: 366 HQAVALRVQSDMSIFYNCQMDGYQDTLYTHTHRQFYRDCTITGTIDFIFGNAAVVFQNCK 425

Query: 423 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 482
           I VRKP+ NQQCIVTAQGR+QRKEPTAIILQNC ISSAPD+FP+RH  KA+LGRPWKQYS
Sbjct: 426 IFVRKPLPNQQCIVTAQGRSQRKEPTAIILQNCLISSAPDYFPVRHTYKAYLGRPWKQYS 485

Query: 483 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 542
           RTII+Q +IDDLIQPEGWLPWTG+FALNTLFY+E N RG G+  D RVKW+GIK+IT EH
Sbjct: 486 RTIILQCKIDDLIQPEGWLPWTGDFALNTLFYSEYNCRGAGSKKDNRVKWRGIKEITPEH 545

Query: 543 ALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           A+DFTA  FIRG+PWIKPTGVPY+SGMM +
Sbjct: 546 AIDFTAGLFIRGNPWIKPTGVPYSSGMMTL 572

[Close]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL
Match: A0A6J1C8E9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009179 PE=3 SV=1)

HSP 1 Score: 877 bits (2265), Expect = 0.0
Identity = 438/580 (75.52%), Postives = 491/580 (84.66%), Query Frame = 0


Query: 1   MSD-QGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKA 60
           MSD  G NKKAALIGVSSLFLVAMVVAVAVGVN++    SG        EI+TSTK+VKA
Sbjct: 1   MSDGDGGNKKAALIGVSSLFLVAMVVAVAVGVNKSPDGGSG--------EITTSTKSVKA 60

Query: 61  LCQPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAK 120
           +CQPTDY ETCEK L  A  NTSDPRELIK GFN AV ++++AI NS+TLK AA+DP AK
Sbjct: 61  ICQPTDYPETCEKTLEAAAVNTSDPRELIKVGFNTAVEQLRFAISNSSTLKSAAADPKAK 120

Query: 121 QALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFEN 180
            ALD CGEL DYAIDDL  SF +ITDNFDM K+DDY+EDLKVWLS ALTYQ  C+DGFEN
Sbjct: 121 LALDDCGELADYAIDDLKNSFNKITDNFDMDKIDDYMEDLKVWLSAALTYQGICLDGFEN 180

Query: 181 VTGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMT-EESN----EQR 240
           VTGDT EKM  +L+ S+ELT NGL MV EV+SI+TSFGLPAIGRRLMT +ESN    E  
Sbjct: 181 VTGDTREKMKGVLKMSQELTTNGLTMVDEVSSIMTSFGLPAIGRRLMTTKESNANAEETE 240

Query: 241 EEPSWVRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVP----KKSNKTFVIYV 300
            EPSWV  RR LLQA+  NIKAD VVA+DGSGKYKT+  AL D+P      +NKTFVI++
Sbjct: 241 SEPSWVSQRRALLQASPGNIKADVVVAQDGSGKYKTINQALKDIPTAKDNPTNKTFVIHI 300

Query: 301 KAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDI 360
           KAG+Y+EQV+++K MTWVMM+GDGPTKTKIT   N+IDG  TFKTATV+VIG+NFI KD+
Sbjct: 301 KAGIYKEQVLIDKHMTWVMMVGDGPTKTKITGSLNFIDGITTFKTATVAVIGTNFIGKDL 360

Query: 361 GFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFG 420
           GFENSAGAAKHQAVALRVQSDMS+FYNCQMDGYQDTLYTHA RQFYRDCTITGTIDFIFG
Sbjct: 361 GFENSAGAAKHQAVALRVQSDMSIFYNCQMDGYQDTLYTHASRQFYRDCTITGTIDFIFG 420

Query: 421 NGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKA 480
           N A +FQNCKILVRKPM+NQQCIVTAQGRTQR+EPTAIILQNC I+S P +FP+R +NKA
Sbjct: 421 NAAAIFQNCKILVRKPMENQQCIVTAQGRTQRREPTAIILQNCVIASDPAYFPVRMVNKA 480

Query: 481 FLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKW 540
           +LGRPWK+YSRTIIMQSQIDDLIQPEGWLPW GNFAL TLFYAE NNRGPGAA DKRVKW
Sbjct: 481 YLGRPWKEYSRTIIMQSQIDDLIQPEGWLPWQGNFALKTLFYAEFNNRGPGAAKDKRVKW 540

Query: 541 KGIKKITMEHALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570
           +GIK+IT EHA+DFT  RF+RGDPWIK TG+PYTSGMMAV
Sbjct: 541 RGIKQITPEHAVDFTVGRFLRGDPWIKRTGIPYTSGMMAV 572

[Close]

BLAST of Cucsat.G565 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-172
Identity = 295/571 (51.66%), Postives = 404/571 (70.75%), Query Frame = 0


Query: 3   DQGKNKKA-ALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 62
           D+ K K+   +I +SS+ L++MVVAV VGV+ N  +    GDS   AE++ S KAVK +C
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD----GDSKGKAEVNASVKAVKDVC 66

Query: 63  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQA 122
            PTDY++TCE  L + G NT+DP EL+K  FNV + +I  A   S T+ E   D   + A
Sbjct: 67  APTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMA 126

Query: 123 LDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVT 182
           LD C ELMDYA+D+L  SF+ +   F+   LD+ + +L++WLS A++++ETC++GF+   
Sbjct: 127 LDQCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQ 186

Query: 183 GDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAIGRRLMTEESNEQREEPSWVR 242
           G+ GE M K L+T+ ELT NGL ++SE+++ +    +P +  R +  E       PSWV 
Sbjct: 187 GNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEG-----FPSWVD 246

Query: 243 DR-RGLLQATGA--NIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQ 302
            R R LLQA  A  ++K D VVA+DGSG+YKT+  AL  VPKK N TFV+++KAG+Y+E 
Sbjct: 247 QRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEY 306

Query: 303 VMVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGA 362
           V V K+M+ ++ IGDGP KT I+  KNY DG  T++TATV+++G+ FIAK+IGFEN+AGA
Sbjct: 307 VQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGA 366

Query: 363 AKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQN 422
            KHQAVA+RVQSD S+F+NC+ DGYQDTLYTH+HRQF+RDCTI+GTIDF+FG+ A VFQN
Sbjct: 367 IKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQN 426

Query: 423 CKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQ 482
           C +LVRKP+ NQ C +TA GR   +E T  + Q C I+  PD+  ++  +KA+LGRPWK+
Sbjct: 427 CTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKE 486

Query: 483 YSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITM 542
           YSRTIIM + I D +QP+GW PW G+F L TLFY+E+ N GPG+A   RV W GIK ++ 
Sbjct: 487 YSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSE 546

Query: 543 EHALDFTAARFIRGDPWIKPTGVPYTSGMMA 570
           E  L FT A++I+GD WI   GVPYT+G++A
Sbjct: 547 EDILKFTPAQYIQGDDWIPGKGVPYTTGLLA 567

[Close]

BLAST of Cucsat.G565 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 598.2 bits (1541), Expect = 6.9e-171
Identity = 292/569 (51.32%), Postives = 395/569 (69.42%), Query Frame = 0


Query: 3   DQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQ 62
           D  + K+  +I +SS+ L++MVVAV +GV+ N        D+A   EI+TS KA+K +C 
Sbjct: 9   DAKRKKRYVIISISSVLLISMVVAVTIGVSVNK------SDNAGDEEITTSVKAIKDVCA 68

Query: 63  PTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQAL 122
           PTDY+ETCE  L +   +TSDP EL+K  FN  + +I      S T+ E   DP AK AL
Sbjct: 69  PTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMAL 128

Query: 123 DACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTG 182
           D C ELMDYAI +L  SF+ +   F+  K+D+ +  L++WLS  +++++TC+DGF+   G
Sbjct: 129 DQCKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQG 188

Query: 183 DTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-GRRLMTEESNEQREEPSWVR 242
           + GE + K L+T+ +LT NGL MV+E+++ L    +P +  RRL+++E       PSW+ 
Sbjct: 189 NAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEF------PSWMD 248

Query: 243 DR-RGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQVM 302
            R R LL A  + +K D VVA+DGSG+YKT+  ALN VPKK N TFV+++K G+Y+E V 
Sbjct: 249 ARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQ 308

Query: 303 VEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAAK 362
           V +SMT ++ IGDGP KT I+  K+Y DG  T+KTATV+++G +FIAK+I FEN+AGA K
Sbjct: 309 VNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIK 368

Query: 363 HQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNCK 422
           HQAVA+RV +D S+FYNC+ DGYQDTLY H+HRQFYRDCTI+GTIDF+FG+ A VFQNC 
Sbjct: 369 HQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCT 428

Query: 423 ILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQYS 482
           +LVRKP+ NQ C +TA GR   +E T  +LQ C I   PD+  ++  +K +LGRPWK+YS
Sbjct: 429 LLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYS 488

Query: 483 RTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITMEH 542
           RTIIM + I D + PEGW PW G F LNTLFY+E+ N GPGAA  KRV W GIKK++ E 
Sbjct: 489 RTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEE 548

Query: 543 ALDFTAARFIRGDPWIKPTGVPYTSGMMA 570
            L FT A++I+GD WI   GVPY  G+ +
Sbjct: 549 ILKFTPAQYIQGDAWIPGKGVPYILGLFS 564

[Close]

BLAST of Cucsat.G565 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 583.6 bits (1503), Expect = 1.8e-166
Identity = 291/571 (50.96%), Postives = 397/571 (69.53%), Query Frame = 0


Query: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65
           K KK  + GV +  LV MVVAV +  +RN+ ++    +     +I T+T AV+A+C PTD
Sbjct: 8   KKKKCIIAGVITALLVLMVVAVGITTSRNTSHS----EKIVPVQIKTATTAVEAVCAPTD 67

Query: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTT--LKEAASDPMAKQALD 125
           Y+ETC  +L +A  +++ P +LIK GFNV +  I+ +I  ++     +AA+D   K AL+
Sbjct: 68  YKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALE 127

Query: 126 ACGELMDYAIDDLVISFQRITDNFD---MQKLDDYIEDLKVWLSGALTYQETCIDGFENV 185
            C +LM+ A DDL    ++  DNFD   + +++D++EDL+VWLSG++ YQ+TC+D FE  
Sbjct: 128 LCEKLMNDATDDL----KKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEET 187

Query: 186 TGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-------GRRLMTEESNEQ 245
                + M K+ +TS+ELT NGL M++ ++++L  F +  +        R+L++ E    
Sbjct: 188 NSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG-- 247

Query: 246 REEPSWVRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAG 305
              PSWV      L AT   +KA+ VVA DGSG+YKT+  ALN VPK + K FVIY+K G
Sbjct: 248 --IPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQG 307

Query: 306 VYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYIDG-TPTFKTATVSVIGSNFIAKDIGF 365
           VY E+V V K MT V  IGDGPTKTKIT   NY  G   T+ TATV++ G NF AK+IGF
Sbjct: 308 VYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGF 367

Query: 366 ENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNG 425
           EN+AG   HQAVALRV +D++VFYNCQ+DGYQDTLY H+HRQF+RDCT++GT+DFIFG+G
Sbjct: 368 ENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDG 427

Query: 426 AVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFL 485
            VV QNC I+VRKPM +Q C++TAQGR+ ++E T ++LQNC I+  P + P++ INKA+L
Sbjct: 428 IVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYL 487

Query: 486 GRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKG 545
           GRPWK++SRTIIM + IDD+I P GWLPW G+FALNTL+YAE  N GPG+   +RVKW G
Sbjct: 488 GRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPG 547

Query: 546 IKKITMEHALDFTAARFIRGDPWIKPTGVPY 564
           IKK++ + AL FT ARF+RG+ WI P  VPY
Sbjct: 548 IKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of Cucsat.G565 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 516.2 bits (1328), Expect = 3.5e-146
Identity = 267/578 (46.19%), Postives = 387/578 (66.96%), Query Frame = 0


Query: 2   SDQGKNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALC 61
           SD  K KK  + G  S FLV MVV+VAV  +++SP             I  +TKAV+A+C
Sbjct: 3   SDGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDEN-------HIRKTTKAVQAVC 62

Query: 62  QPTDYQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASD--PMAK 121
            PTD+++TC  +L  A  ++ DP +LIK GF V +  I  ++  ++   +A +D  P AK
Sbjct: 63  APTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAK 122

Query: 122 QALDACGELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFEN 181
            A + C +LM  AIDDL    + +   F + +++ ++EDL+VWLSG++ +Q+TC+D F  
Sbjct: 123 GAFELCEKLMIDAIDDLK---KCMDHGFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGE 182

Query: 182 VTGDTGEKMTKLLETSKELTINGLGMVSEVTSILTSFGLPAI-------GRRLMTEESNE 241
           +  +  + M K+ +TS+EL+ N L MV+ +++++ +  L  +        R+L++ E + 
Sbjct: 183 IKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDS- 242

Query: 242 QREEPSWV-RDRRGLLQATGAN---IKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVI 301
               P+WV  + R L+ A G     +KA+AVVA+DG+G++KT+T ALN VPK +   F+I
Sbjct: 243 ---IPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFII 302

Query: 302 YVKAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNY-IDGTPTFKTATVSVIGSNFIA 361
           ++K G+Y+E+V V K M  V  IGDGP KT IT   N+ I    TF TAT+++ G +F A
Sbjct: 303 HIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTA 362

Query: 362 KDIGFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDF 421
           K+IG EN+AG    QAVALRV +D +VF++CQ+DG+QDTLY H+HRQFYRDCT++GT+DF
Sbjct: 363 KNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDF 422

Query: 422 IFGNGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHI 481
           IFG+   + QNCKI+VRKP   Q C+VTAQGR+  +E T ++L  C I+  P + P++ +
Sbjct: 423 IFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSV 482

Query: 482 NKAFLGRPWKQYSRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKR 541
           NKA+LGRPWK++SRTIIM++ IDD+I P GWLPW+G+FAL TL+YAE  N GPG+   +R
Sbjct: 483 NKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQR 542

Query: 542 VKWKGIKKITMEHALDFTAARFIRGDPWIKPTGVPYTS 566
           VKW GIKK+T + AL +T  RF+RGD WI  T VPYT+
Sbjct: 543 VKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of Cucsat.G565 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.0 bits (1151), Expect = 1.2e-125
Identity = 267/704 (37.93%), Postives = 373/704 (52.98%), Query Frame = 0


Query: 9   KAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTDYQE 68
           K  L+GV++L ++AMV+ V    + NSP+         +  + T T  V  +C  TDY++
Sbjct: 3   KYVLLGVTAL-IMAMVICVEAN-DGNSPSRKMEEVHRESKLMITKT-TVSIICASTDYKQ 62

Query: 69  TCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAI--GNSTTLKEAASDPMAKQALDACG 128
            C  +L  A   + DPR LI++ F++A+  I+  I  G       A +D   ++AL+ C 
Sbjct: 63  DCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 129 ELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTGE 188
           ELMD AIDDL  +  +    F   +L D++EDL VWLSG++TYQ+TCIDGFE +  +   
Sbjct: 123 ELMDDAIDDLRKTRDKFR-GFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 189 KMTKLLETSKELTINGLGMVSEVTSILTSFGLP------------AIGRRLMTEES---- 248
            M +++   + LT NGL + + +  +L +F +P             +G     +ES    
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 249 ------------------------------------------------------------ 308
                                                                       
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 309 ------------------------------------------------------------ 368
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 369 -----NEQREEPSWV--RDRRGLLQATGAN-IKADAVVAKDGSGKYKTVTAALNDVPKKS 428
                +E+ E P WV    RR L +    N IKA+ VVAKDGSGK KT+  AL  VP K+
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKN 422

Query: 429 NKTFVIYVKAGVYQEQVMVEKSMTWVMMIGDGPTKTKITAGKNYI-DGTPTFKTATVSVI 488
            K FVI++K GVY+E+V V K M  VM +GDGPTKT IT    ++ D   T++TA+V+V 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 489 GSNFIAKDIGFENSAGAAKHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTI 548
           G  F+AKDIGFEN+AGAA+HQAVALRV +D +VF+NC M+GYQDTLY H HRQFYR+C +
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 549 TGTIDFIFGNGAVVFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDF 566
           +GTIDF+FG+   VFQNC+ ++R+PM++QQCIVTAQGR  R+E T I++ N  I+    +
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

The following BLAST results are available for this feature:

BLAST of Cucsat.G565 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GX86	1.8e-171	51.66	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q3E8Z8	9.7e-170	51.32	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	2.5e-165	50.96	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	4.9e-145	46.19	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	1.6e-124	37.93	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cucsat.G565 vs. NCBI nr


Match Name	Identity	Description
XP_004134493.2	100.00	pectinesterase [Cucumis sativus] >KGN57132.1 hypothetical protein Csa_009573 [Cu...	[more]
KAA0049514.1	90.35	pectinesterase-like [Cucumis melo var. makuwa] >TYK16194.1 pectinesterase-like [...	[more]
XP_008438906.2	90.16	PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo]	[more]
XP_038874833.1	88.03	pectinesterase-like [Benincasa hispida]	[more]
XP_023529046.1	76.05	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G565 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0L930	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G164510 PE=3 SV=1	[more]
A0A5A7U2Q1	90.35	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00122...	[more]
A0A1S3AY67	90.16	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483865 PE=3 SV=1	[more]
A0A6J1GYH5	76.32	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457997 PE=3 SV=1	[more]
A0A6J1C8E9	75.52	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009179 PE=3 SV=1	[more]

BLAST of Cucsat.G565 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05610.1	1.3e-172	51.66	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G27870.1	6.9e-171	51.32	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	1.8e-166	50.96	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	3.5e-146	46.19	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	1.2e-125	37.93	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 52..205 e-value: 9.5E-33 score: 124.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 51..204 e-value: 3.0E-24 score: 83.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 56..203 e-value: 3.0E-24 score: 86.1
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 257..553 e-value: 1.5E-141 score: 470.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 51..209 e-value: 6.0E-31 score: 109.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 55..209
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 251..567 e-value: 9.7E-120 score: 401.7
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 12..567
None	No IPR available	PANTHER	PTHR31707:SF131	PECTINESTERASE	coord: 12..567
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 59..211 e-value: 5.75685E-41 score: 143.348
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 282..301
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 401..410
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 254..566

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G565.T1	Cucsat.G565.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity