Cucsat.G5303 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G5303
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	ctg1251: 94610 .. 97840 (-)
RNA-Seq Expression	Cucsat.G5303
Synteny	Cucsat.G5303

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGAAAAACCGTCATTTCATTCATGTCTGTCTCTTCACTCCACTGCCTGGTTCCGTCCCCCATCTCCAAACTCTTTGATGGTCATCTCCGGACGACGTCCAATTCCATCACAACCACTGCCCCCTCGTGTCCGTCTCCTCACTCTAGGGCCGTCGAAACCGCTTCTACTGGATGATGAAACGTCCTCTGTCCTAATTTGTTCAATTCGTGCTCATAATCTCCAATCCGCCGCATTCTCTTCAATATTCAATCCTCAGCCGTCACCTCCTCTACCCGGTAGGTCGTCTATTCCTTTTCTATCTTTTGGGCAAACACCCGTATATGAAGTATGTGAATTAAACTAGACCAAAAAATATTTAAGGCTTGAAGCTGAAAGAGAGCACTATTTGAGAATGAGAATGAGTGAAATGAGTTTTCTCAGGTTTTCTTGTCTTGTTCTTGCCTATTATGATAATGGGTGAAATGAGTTTTTCTTATTTGTTTGTTTTCTTTATGATTTTGTTGTAGCTGTGGATTTCATTTTTTGGATTGCTTATGGAGTTGTGTTCAGTTATGGATTTGGTTTGTTTTCCTCTGTGTTTGAGGTGGAATTTTATATATGTGAAACACAGAATCTTGATGTTTTGTTTATTCATTTTGTGGTTACTGTGTTAATCTTTTATTTTATTTATTTGTTTGTTATAAATTGGAATGAGAATTATAAGTCATAGAAACTTTTGGTTCTCTAATTTTTACAATTTAGTTGGTTTAATTAATCAGAGATGGGGTATGGTAAGGGTAAGTTTTCTTCTACAATGTTCTTGGGAATAAAACTTTTATATATATATATATATATATATATATATATATATATATATATATATAATGATAAAAGTCATTATTCTATAGATCAAATGAAAAAGTACAATATTTGGAGCCAGAAGTGAAAACAGAGGCAAAACTATCTACCAAAAAGGGGTTTTACAGTTGAGAGTGAGAAACAAAGATTTTTTTTTTCAATTTATTAAATGTGGAGGTGAACTTTTTTTTTTCTACTTTTCTTTCGGTTCTCAACTTGATGAACTTCAAGGAGTGTGAGGAATTTTGGGTGGATTGAAAATCTGAAAAATATCGATCAAGTAGTTGGTATTGAAATTTAGGGGATCATTTGAGTTATGAAGTATTGTTTAGGATACTTTTAAGACTATGCTGCAAACCAACTGTTAAGACTAAGATTGATGTTGATTAGGATACTTTCTCGGTAGAGTAGTTGATAGCAATATTGTGAAGTCTAATACGTTTCATGTCATATAGTATCCTACTAAGTTGTGACCCCCTTTCTTAATAATGTTATGGAAATTTCAATACAGAGTTGTGACCCCCTCTCTTAAAACACGCTAATTTCAATGACCCCCTCTCTTAAAACTTGCTAATTTCAATGTTGAAGTTTCTCCAAAAACTAATAAATTAAAAGTGCGAACTATTGTCGTGTAGGCATGCCATAGGCATTGGCTCTCAACTCTCAAGGCTGAAGCCAACAACGGAGAGAAAATGGGGGCAGGGTGGAGCAGTAGTGTAGTAGTTAGATAGAAAATGGGGGCATTGACTCTTAACTCTCAAGTTTCAACTCTCACACTTGTTTTTTTTTTTTTTTTTTGTGAGTTGATTATCTCTGCTGGAAATATACTTTTGTGTGTGTTGGTTTTTCTTACCACAATTTAATCAAATTTAGCTCTCTATATAGTACTATTTTGTATTTTTGATATAATTAATTGAAATAGAAATTTTTTGAATGCTATCATATTATGCATATCAAATAAATTATTTCTGGACAATAATACTCTTCAACATGCTGTAAATTTGATTAGCATTTAAGCTTTTTAAGGGTGGTATATTTGAGGATTTTCAGGATCGGATTATGTACCATTTTGAAAGTTTTAAAAGTTTTAAAAGTTGTTGCCATTTATCTTTTTTGGGGCCAGCAAAGAAAGTTCTTGGAATTTGAATAGTTCGAGATAGAGCATCCAAGAAGTTATACATGTCACAGGAGCAATACATAGAGAAAGTACTTGAACATTTCAAGATGAATCAAGCAAAACCAGTTAGTTCCCCTTTACCCAGTCACTTCAAACTGATCAATAAACAAAGCCCTTCTACAGATAAAGAGAAGGAGGATATGAGTAAGGTCCCATATGCTTCAGCAGTTGGAAGCCTAATGTATGCCATGGTATGTATTAGACCTGATATTGCTCATGTTGTTGGTGTTGTTAGTCGTTTTATGTCTAATCCAGGAAAACAACATTGGGAGGCAAAGTGCATCATGAGATATTTGAGAGGTACTTTCAGTTTGAAGCTCACATTTGGGGATGGAAAGCCAGTACTTGCTGGGTATACTGATTCAGATATGGCAAGGGACTTAGGCAGCAAAAAGTCTACTTCTGGTTACTTGATGATATTTGCAGGTGGTGCAGTGTCTTGGCAGTCAAGGTTGCAGAAATGTGTTTCCCTTTCTACAACTAAAGCAAAGTATATTGCAGCAGCAAAAGCATGTAAAGATATGTTGTGGATGAAGCGCTTTGTACAAGAGCTTGGCTTCAAGTAACAACGATATGTGATATATTGTGACAATCTGCTATTCACCTTGGTAATAATATGCTTCATTTCATTCAAGAACAAGGCATATTGATGTGAGATATCACTGGCTTAGAGATGCTTTAAATGATGAATTGTTTGAGCTTGAGAATATACACAATGATCATAATATATCTGTTATGTTGACAAAGAATCTATCAAGATCGAAGCTTGAGTTTTGTCGTTCCATAGTGGGAATGACAAATTCATCCTCAAAGTGAAATAGGGGGAGATTTGTTGGGCTTATTAAACTTGAAGCCCAGCCCATTAGCATATTAGGTTAATTTTCATTATGAAATTAACTAACGTGCAAAAACGGAAAAAATGTGTGTGATTGCAATAGAGTGGAAAGAAAGAAAAACTAAATCTTTGTGTGGTTCATATTTCAGCCTTTTTGGGATCGATTCGTTGTCCGATCAAGCTGAAATTTTGACAGTGCAATCTTGGCACAAGATTCTTCATTATGGATGGTGGAGATTTTGTTTGGAGCTATCTATTCTAGTTGATAGTTGGTTACCACAAATTGTGAGATATTTATTTTCTTTCATTGTTATTGTTGTTTGTTGTGACTAGAGAGAACTTTGTTATACCTATTAAAGATTATAATGGAGATTTTGACT

Coding sequence (CDS)

ATGTCACAGGAGCAATACATAGAGAAAGTACTTGAACATTTCAAGATGAATCAAGCAAAACCAGTTAGTTCCCCTTTACCCAGTCACTTCAAACTGATCAATAAACAAAGCCCTTCTACAGATAAAGAGAAGGAGGATATGAGTAAGGTCCCATATGCTTCAGCAGTTGGAAGCCTAATGTATGCCATGGTATGTATTAGACCTGATATTGCTCATGTTGTTGGTGTTGTTAGTCGTTTTATGTCTAATCCAGGAAAACAACATTGGGAGGCAAAGTGCATCATGAGATATTTGAGAGGTACTTTCAGTTTGAAGCTCACATTTGGGGATGGAAAGCCAGTACTTGCTGGGTATACTGATTCAGATATGGCAAGGGACTTAGGCAGCAAAAAGTCTACTTCTGGTTACTTGATGATATTTGCAGGTGGTGCAGTGTCTTGGCAGTCAAGGTTGCAGAAATGTGTTTCCCTTTCTACAACTAAAGCAAAGTATATTGCAGCAGCAAAAGCATGTAAAGATATGTTGTGGATGAAGCGCTTTGTACAAGAGCTTGGCTTCAAGTAA

Protein sequence

MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLMYAMVCIRPDIAHVVGVVSRFMSNPGKQHWEAKCIMRYLRGTFSLKLTFGDGKPVLAGYTDSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKRFVQELGFK

Homology

[Close]

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 3.3e-66
Identity = 122/186 (65.59%), Postives = 152/186 (81.72%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
            +SQE+YIE+VLE F M  AKPVS+PL  H KL  K  P+T +EK +M+KVPY+SAVGSLM
Sbjct: 1060 LSQEKYIERVLERFNMKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKVPYSSAVGSLM 1119

Query: 61   YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
            YAMVC RPDIAH VGVVSRF+ NPGK+HWEA K I+RYLRGT    L FG   P+L GYT
Sbjct: 1120 YAMVCTRPDIAHAVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPILKGYT 1179

Query: 121  DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
            D+DMA D+ ++KS++GYL  F+GGA+SWQS+LQKCV+LSTT+A+YIAA +  K+M+W+KR
Sbjct: 1180 DADMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKR 1239

Query: 181  FVQELG 186
            F+QELG
Sbjct: 1240 FLQELG 1245

[Close]

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 63/133 (47.37%), Postives = 93/133 (69.92%), Query Frame = 0


Query: 47  MSKVPYASAVGSLMYAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLK 106
           M  VPY SAVG++MY MV  RPD+A  VGV+S+F S+P   HW+A K ++RYL+ T +  
Sbjct: 1   MKNVPYLSAVGAIMYLMVVTRPDLAAAVGVLSQFASDPCPTHWQALKRVLRYLQSTQTYG 60

Query: 107 LTF-GDGKPVLAGYTDSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKY 166
           L F   G   L GY+D+D A D+ S++STSGYL    GG VSW+S+ Q+ V+LS+T+ +Y
Sbjct: 61  LEFTRAGTAKLVGYSDADWAGDVESRRSTSGYLFKLNGGCVSWRSKKQRTVALSSTEDEY 120

Query: 167 IAAAKACKDMLWM 178
           +A ++A ++ +W+
Sbjct: 121 MALSEATQEAVWL 133

[Close]

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 67/192 (34.90%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
            +SQ  Y++K+L  F M     VS+PLPS    IN +  ++D++       P  S +G LM
Sbjct: 1137 LSQSAYVKKILSKFNMENCNAVSTPLPSK---INYELLNSDED----CNTPCRSLIGCLM 1196

Query: 61   YAMVCIRPDIAHVVGVVSRFMSNPGKQHWE-AKCIMRYLRGTFSLKLTFGDG---KPVLA 120
            Y M+C RPD+   V ++SR+ S    + W+  K ++RYL+GT  +KL F      +  + 
Sbjct: 1197 YIMLCTRPDLTTAVNILSRYSSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKII 1256

Query: 121  GYTDSDMARDLGSKKSTSGYLM-IFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDML 180
            GY DSD A     +KST+GYL  +F    + W ++ Q  V+ S+T+A+Y+A  +A ++ L
Sbjct: 1257 GYVDSDWAGSEIDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREAL 1316

Query: 181  WMKRFVQELGFK 188
            W+K  +  +  K
Sbjct: 1317 WLKFLLTSINIK 1321

[Close]

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-18
Identity = 66/190 (34.74%), Postives = 102/190 (53.68%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPL-PSHFKLINKQSPSTDKEKEDMSKVPYASAVGSL 60
            +SQ +YI  +L    M  AKPV++P+ PS    +   +  TD  +       Y   VGSL
Sbjct: 1200 LSQRRYILDLLARTNMITAKPVTTPMAPSPKLSLYSGTKLTDPTE-------YRGIVGSL 1259

Query: 61   MYAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPV-LAG 120
             Y +   RPDI++ V  +S+FM  P ++H +A K I+RYL GT +  +    G  + L  
Sbjct: 1260 QY-LAFTRPDISYAVNRLSQFMHMPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLSLHA 1319

Query: 121  YTDSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWM 180
            Y+D+D A D     ST+GY++      +SW S+ QK V  S+T+A+Y + A    +M W+
Sbjct: 1320 YSDADWAGDKDDYVSTNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWI 1379

Query: 181  KRFVQELGFK 188
               + ELG +
Sbjct: 1380 CSLLTELGIR 1381

[Close]

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.4e-18
Identity = 61/189 (32.28%), Postives = 95/189 (50.26%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
            +SQ +Y   +L    M  AKPV++P+ +  KL            E      Y   VGSL 
Sbjct: 1183 LSQRRYTLDLLARTNMLTAKPVATPMATSPKLTLHSGTKLPDPTE------YRGIVGSLQ 1242

Query: 61   YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPV-LAGY 120
            Y +   RPD+++ V  +S++M  P   HW A K ++RYL GT    +    G  + L  Y
Sbjct: 1243 Y-LAFTRPDLSYAVNRLSQYMHMPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAY 1302

Query: 121  TDSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMK 180
            +D+D A D     ST+GY++      +SW S+ QK V  S+T+A+Y + A    ++ W+ 
Sbjct: 1303 SDADWAGDTDDYVSTNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSELQWIC 1362

Query: 181  RFVQELGFK 188
              + ELG +
Sbjct: 1363 SLLTELGIQ 1364

[Close]

BLAST of Cucsat.G5303 vs. NCBI nr
Match: RVW24009.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 293 bits (749), Expect = 1.23e-92
Identity = 145/188 (77.13%), Postives = 165/188 (87.77%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           M QEQYIEKVL  F M +AK VSSPL SHFKL ++ SPSTDKEKEDM +VPYASAVGSLM
Sbjct: 307 MLQEQYIEKVLARFNMGKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLM 366

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAMVC RPDIA+ VGVVSRF+SNPG+ HWEA K IMRYLRGT  LKLTFG GKP+L GYT
Sbjct: 367 YAMVCTRPDIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYT 426

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           DSDMA D+ +++STSGYLM F+GGAVSWQSRLQKCV+LSTT+A+YIAAA+ACK++LWMK 
Sbjct: 427 DSDMAGDVDNRRSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKC 486

Query: 181 FVQELGFK 187
           F+QELGFK
Sbjct: 487 FMQELGFK 494

[Close]

BLAST of Cucsat.G5303 vs. NCBI nr
Match: VVA40656.1 (PREDICTED: Retrovirus-related [Prunus dulcis])

HSP 1 Score: 276 bits (706), Expect = 4.63e-89
Identity = 132/186 (70.97%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 123 LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 182

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 183 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 242

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+GGAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 243 DADMAGDLDSRKSTSGYLITFSGGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 302

Query: 181 FVQELG 185
           F+QELG
Sbjct: 303 FLQELG 308

[Close]

BLAST of Cucsat.G5303 vs. NCBI nr
Match: VVA40655.1 (PREDICTED: Retrovirus-related [Prunus dulcis])

HSP 1 Score: 276 bits (706), Expect = 1.38e-88
Identity = 132/186 (70.97%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 160 LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 219

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 220 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 279

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+GGAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 280 DADMAGDLDSRKSTSGYLITFSGGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 339

Query: 181 FVQELG 185
           F+QELG
Sbjct: 340 FLQELG 345

[Close]

BLAST of Cucsat.G5303 vs. NCBI nr
Match: RVW24680.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 293 bits (750), Expect = 1.42e-88
Identity = 145/188 (77.13%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
            M QEQYIEKVL  F M++AK VSSPL SHFKL ++ SPSTDKEKEDM +VPYASAVGSLM
Sbjct: 947  MLQEQYIEKVLARFNMSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLM 1006

Query: 61   YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
            YAMVC RPDIA+ VGVVSRF+SNPG+ HWEA K IMRYLRGT  LKLTFG GKP+L GYT
Sbjct: 1007 YAMVCTRPDIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYT 1066

Query: 121  DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
            DSDMA D+ +++STSGYLM F+GGAVSWQSRLQKCV+LSTT+A+YIAAA+ACK++LWMK 
Sbjct: 1067 DSDMAGDVDNRRSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKC 1126

Query: 181  FVQELGFK 187
            F+QELGFK
Sbjct: 1127 FMQELGFK 1134

[Close]

BLAST of Cucsat.G5303 vs. NCBI nr
Match: VVA10754.1 (PREDICTED: Retrovirus-related Pol poly from [Prunus dulcis])

HSP 1 Score: 273 bits (698), Expect = 2.22e-88
Identity = 131/186 (70.43%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 83  LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 142

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 143 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 202

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+ GAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 203 DADMAGDLDSRKSTSGYLITFSRGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 262

Query: 181 FVQELG 185
           F+QELG
Sbjct: 263 FLQELG 268

[Close]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL
Match: A0A438CLF9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2520 PE=4 SV=1)

HSP 1 Score: 293 bits (749), Expect = 5.94e-93
Identity = 145/188 (77.13%), Postives = 165/188 (87.77%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           M QEQYIEKVL  F M +AK VSSPL SHFKL ++ SPSTDKEKEDM +VPYASAVGSLM
Sbjct: 307 MLQEQYIEKVLARFNMGKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLM 366

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAMVC RPDIA+ VGVVSRF+SNPG+ HWEA K IMRYLRGT  LKLTFG GKP+L GYT
Sbjct: 367 YAMVCTRPDIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYT 426

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           DSDMA D+ +++STSGYLM F+GGAVSWQSRLQKCV+LSTT+A+YIAAA+ACK++LWMK 
Sbjct: 427 DSDMAGDVDNRRSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKC 486

Query: 181 FVQELGFK 187
           F+QELGFK
Sbjct: 487 FMQELGFK 494

[Close]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL
Match: A0A5E4GLX8 (PREDICTED: Retrovirus-related OS=Prunus dulcis OX=3755 GN=ALMOND_2B013925 PE=4 SV=1)

HSP 1 Score: 276 bits (706), Expect = 2.24e-89
Identity = 132/186 (70.97%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 123 LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 182

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 183 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 242

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+GGAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 243 DADMAGDLDSRKSTSGYLITFSGGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 302

Query: 181 FVQELG 185
           F+QELG
Sbjct: 303 FLQELG 308

[Close]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL
Match: A0A5E4GLA2 (PREDICTED: Retrovirus-related OS=Prunus dulcis OX=3755 GN=ALMOND_2B013925 PE=4 SV=1)

HSP 1 Score: 276 bits (706), Expect = 6.70e-89
Identity = 132/186 (70.97%), Postives = 161/186 (86.56%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 160 LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 219

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 220 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 279

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+GGAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 280 DADMAGDLDSRKSTSGYLITFSGGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 339

Query: 181 FVQELG 185
           F+QELG
Sbjct: 340 FLQELG 345

[Close]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL
Match: A0A438CN91 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3654 PE=4 SV=1)

HSP 1 Score: 293 bits (750), Expect = 6.86e-89
Identity = 145/188 (77.13%), Postives = 166/188 (88.30%), Query Frame = 0


Query: 1    MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
            M QEQYIEKVL  F M++AK VSSPL SHFKL ++ SPSTDKEKEDM +VPYASAVGSLM
Sbjct: 947  MLQEQYIEKVLARFNMSKAKVVSSPLASHFKLSSRHSPSTDKEKEDMRRVPYASAVGSLM 1006

Query: 61   YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
            YAMVC RPDIA+ VGVVSRF+SNPG+ HWEA K IMRYLRGT  LKLTFG GKP+L GYT
Sbjct: 1007 YAMVCTRPDIAYAVGVVSRFLSNPGRHHWEAVKWIMRYLRGTSKLKLTFGSGKPILVGYT 1066

Query: 121  DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
            DSDMA D+ +++STSGYLM F+GGAVSWQSRLQKCV+LSTT+A+YIAAA+ACK++LWMK 
Sbjct: 1067 DSDMAGDVDNRRSTSGYLMTFSGGAVSWQSRLQKCVALSTTEAEYIAAAEACKELLWMKC 1126

Query: 181  FVQELGFK 187
            F+QELGFK
Sbjct: 1127 FMQELGFK 1134

[Close]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL
Match: A0A5E4E4T7 (PREDICTED: Retrovirus-related Pol poly from OS=Prunus dulcis OX=3755 GN=ALMOND_2B017776 PE=4 SV=1)

HSP 1 Score: 273 bits (698), Expect = 1.07e-88
Identity = 131/186 (70.43%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQE YIEKVL+ F M++AKPVS+P PSHFKL +KQSP+ +KEKE+M+ VPY+SAVGSLM
Sbjct: 83  LSQESYIEKVLKRFNMDKAKPVSTPFPSHFKLSSKQSPTCEKEKENMAMVPYSSAVGSLM 142

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWEA-KCIMRYLRGTFSLKLTFGDGKPVLAGYT 120
           YAM+C RPDI H VGVVSRF+S PG++HW A K I+RYLRGT  + L FG GKP L GYT
Sbjct: 143 YAMICTRPDITHAVGVVSRFLSKPGREHWNAVKWILRYLRGTSKMSLCFGGGKPELIGYT 202

Query: 121 DSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLWMKR 180
           D+DMA DL S+KSTSGYL+ F+ GAVSWQS+LQKCV+LSTT+A++IAA +ACK+MLWMKR
Sbjct: 203 DADMAGDLDSRKSTSGYLITFSRGAVSWQSKLQKCVALSTTEAEFIAATEACKEMLWMKR 262

Query: 181 FVQELG 185
           F+QELG
Sbjct: 263 FLQELG 268

[Close]

BLAST of Cucsat.G5303 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 54/178 (30.34%), Postives = 93/178 (52.25%), Query Frame = 0


Query: 1   MSQEQYIEKVLEHFKMNQAKPVSSPLPSHFKLINKQSPSTDKEKEDMSKVPYASAVGSLM 60
           +SQ +Y E++L +  M   KP+S+PLP   KL +  S +   +  D     + S VG+L 
Sbjct: 56  LSQTKYAEQILNNAGMLDCKPMSTPLP--LKLNSSVSTAKYPDPSD-----FRSIVGALQ 115

Query: 61  YAMVCIRPDIAHVVGVVSRFMSNPGKQHWE-AKCIMRYLRGT-FSLKLTFGDGKPVLAGY 120
           Y +   RPDI++ V +V + M  P    ++  K ++RY++GT F       + K  +  +
Sbjct: 116 Y-LTLTRPDISYAVNIVCQRMHEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAF 175

Query: 121 TDSDMARDLGSKKSTSGYLMIFAGGAVSWQSRLQKCVSLSTTKAKYIAAAKACKDMLW 177
            DSD A    +++ST+G+        +SW ++ Q  VS S+T+ +Y A A    ++ W
Sbjct: 176 CDSDWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

The following BLAST results are available for this feature:

BLAST of Cucsat.G5303 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	3.3e-66	65.59	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P0CV72	1.8e-27	47.37	Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P...	[more]
P04146	2.8e-25	34.90	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
Q94HW2	2.0e-18	34.74	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	7.4e-18	32.28	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]

BLAST of Cucsat.G5303 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW24009.1	1.23e-92	77.13	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
VVA40656.1	4.63e-89	70.97	PREDICTED: Retrovirus-related [Prunus dulcis]	[more]
VVA40655.1	1.38e-88	70.97	PREDICTED: Retrovirus-related [Prunus dulcis]	[more]
RVW24680.1	1.42e-88	77.13	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera]	[more]
VVA10754.1	2.22e-88	70.43	PREDICTED: Retrovirus-related Pol poly from [Prunus dulcis]	[more]

BLAST of Cucsat.G5303 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438CLF9	5.94e-93	77.13	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A0A5E4GLX8	2.24e-89	70.97	PREDICTED: Retrovirus-related OS=Prunus dulcis OX=3755 GN=ALMOND_2B013925 PE=4 S...	[more]
A0A5E4GLA2	6.70e-89	70.97	PREDICTED: Retrovirus-related OS=Prunus dulcis OX=3755 GN=ALMOND_2B013925 PE=4 S...	[more]
A0A438CN91	6.86e-89	77.13	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]
A0A5E4E4T7	1.07e-88	70.43	PREDICTED: Retrovirus-related Pol poly from OS=Prunus dulcis OX=3755 GN=ALMOND_2...	[more]

BLAST of Cucsat.G5303 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00810.1	3.5e-15	30.34	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 85..185
None	No IPR available	PANTHER	PTHR11439:SF301	SUBFAMILY NOT NAMED	coord: 85..185
None	No IPR available	CDD	cd09272	RNase_HI_RT_Ty1	coord: 117..187 e-value: 1.03031E-20 score: 81.3605

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G5303.T1	Cucsat.G5303.T1	mRNA
Cucsat.G5303.T2	Cucsat.G5303.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding