Cucsat.G5248 (gene) Cucumber (B10) v3

Overview
NameCucsat.G5248
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCold shock protein 2-like
Locationctg1228: 239946 .. 241880 (-)
RNA-Seq ExpressionCucsat.G5248
SyntenyCucsat.G5248
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTTCCCCCGAATCTCTCTATCCGAGAGACATCCACTTTCGAAAAGTGAATCAATACTCAAATAAAGGGGAGGAAAAATCAATTGTCCGCCATTGGTTCCTTCTGTGAATGACATGCAGCTTATTCATCTAGATATACATTGCTACAAGCTTTTCAATTGTCCTTTCTTTCCCTTTTCTTTCTTTTACACTTTTTCCTTTTCATGGCTGAGCTGAAGAGATCGACCGGTACTGTAAGGTGGTTTAGTGCACAGAAGGGGTTTGGCTTCATAGCTCCAGATGATGCTACCGATGATCTTTTCGTCCACCAAACCTCCATTCGTTCAGAAGGATTCCGGACTCTTTTCGATGGTCAAACTGTTGAGTTTACCATCGATTATGATCAAGATCAACGCGCCAAGGCCGTCGACGTCACTGTAATTGATAGATCTACCAGATATGGAGGAGGCCGCGGGGGTGGGAGAGGTAGAGGTGGTTCTTATGGAAGATTCGGTGGTGGTGGACGTGGTTTTGGCAGAGGAAGATGGAACGAAGGTCCAAGCGGTGGTGGTCGTGGCGGTGGGGAGTGCTACAATTGTGGCCGGATGGGTCATTTGGCGAGGGATTGTTACAGGGGTAACGGCGGTGCTCCTTCCGGCCGTAGATACGACGGTGGTCGAGGTTATGGTGGAGGTGGAGGCGGCAGAGGTTGTTATAATTGTGGAGATACAGGACATTTGGCTAGGGATTGTCAAAACGAAAGCAAATGAAGAATGTCGGTTTTCCTTTAAGCGGTACACATTATACTTTTTCTGAAAAATTATTTTGGTTGGGCTGTTAATTTTTTTATGCGAAATCTGGGATTTTTTAGTGTGTCAGTTACTTCAAGTCTGCAATTGAGGGGAACGAGGATCTGGTGGCAATAAATCTTAAGGCTTCAGTTCGTCTATATTTCTCCTTTTGGGTCGTCCATAATTTGATGTTTATAATTAGATGATGAAACCAATTGTTTGTATATGCTATACCTTTCTGGCATTTCTAATTCATTCTTTCTTTGCTTGTTTTATAGAATCATAATATGATATTTGTAATCTGTCAAAGGTATACGTTCATACCATTTTTTATTTCTTATATACGTTTCACTTTTGTCTATTACTGTAATGGTTGTATTTTTTCGCCCCCATTATTGATTTCTTGGTGCTTTACTGGGATTCTTTGCTTCGAAACTTCAATCATTGGTGTTGAATTGCCAATGGAAGTAATTGTCCTGCACTATGTACTATATTTTTGCCTGTGTTTTCCCCGGAATCTGAATGAAACCAGAGCTCTTAATAATACTTTAGATATCTTTGAAGTTTCTCACAGAGGAAGCTAAACCCATTTCAGGGTTTCACTTATTGTATCTGCTAATAGTTGTGGCTGGATTTGAAATTTCTCCATTTGCCTTGTTTCACTCATGAAAACTCTAAGCATAGAAATCTCAGCTATTGGTGGTGTTGCTCTTGATGTTTAGTAAGATCTTCAATCTTATTTTTCCTGTTCTCTATTGTTTCACATCATCTGATTTTTGGCTTTCTATTCAATGTATGACAACTGTCATTATATTTGAAGCTTGAGAAAATTGGAGGTTGTTTTTGATTCAATTTTGCTATGCTTTCTCAGAACTAGTGAAATCGTGTGGTCGATCTTCTGTTTGACTGAGATGTGATGCTGCTAGATGAAATGCTTGTAGTTTGCTCGTTTTATTTTATTTGCATTGTGTTTTATAGATCTCAATCATCCTTTATTCAACATACAGGCCGTTTATGTAACTGTTATGTTCTTTTATTGGGTTGATTATTGAAAGAATTTTACACAAACGTAATCCATTGAAAAGCAAAAGATATTACCTTATAATCATTTTGTTCTTGCCTTTTCCGAGTGGTATATATCACTGTAATAGTATCCCCATTCAT

Coding sequence (CDS)

ATGGCTGAGCTGAAGAGATCGACCGGTACTGTAAGGTGGTTTAGTGCACAGAAGGGGTTTGGCTTCATAGCTCCAGATGATGCTACCGATGATCTTTTCGTCCACCAAACCTCCATTCGTTCAGAAGGATTCCGGACTCTTTTCGATGGTCAAACTGTTGAGTTTACCATCGATTATGATCAAGATCAACGCGCCAAGGCCGTCGACGTCACTGTAATTGATAGATCTACCAGATATGGAGGAGGCCGCGGGGGTGGGAGAGGTAGAGGTGGTTCTTATGGAAGATTCGGTGGTGGTGGACGTGGTTTTGGCAGAGGAAGATGGAACGAAGGTCCAAGCGGTGGTGGTCGTGGCGGTGGGGAGTGCTACAATTGTGGCCGGATGGGTCATTTGGCGAGGGATTGTTACAGGGGTAACGGCGGTGCTCCTTCCGGCCGTAGATACGACGGTGGTCGAGGTTATGGTGGAGGTGGAGGCGGCAGAGGTTGTTATAATTGTGGAGATACAGGACATTTGGCTAGGGATTGTCAAAACGAAAGCAAATGA

Protein sequence

MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK
Homology
BLAST of Cucsat.G5248 vs. ExPASy Swiss-Prot
Match: Q41188 (Cold shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=CSP2 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.5e-39
Identity = 102/199 (51.26%), Postives = 121/199 (60.80%), Query Frame = 0

Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R  G+V+WF  QKGFGFI PDD  DDLFVHQ+SIRSEGFR+L   + VEF ++ D + R
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNNNR 68

Query: 65  AKAVDVTVIDRST--------------RYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNE 124
            KA+DV+  D +                +GGGRGGGRG GG Y   GGGG G+G      
Sbjct: 69  PKAIDVSGPDGAPVQGNSGGGSSGGRGGFGGGRGGGRGSGGGY---GGGGGGYG------ 128

Query: 125 GPSGGGRGGGECYNCGRMGHLARDCYRGNGG-APSGRRYDGGRGYGGGGGGRG------- 177
           G  GGGRGG +CY CG  GH+ARDC  G GG    G  Y GG GYGGGGGG G       
Sbjct: 129 GRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYGGGGGYGGGGGGYGGGGRGGG 188

BLAST of Cucsat.G5248 vs. ExPASy Swiss-Prot
Match: P27484 (Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 105/210 (50.00%), Postives = 123/210 (58.57%), Query Frame = 0

Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R+ GTV+WFS QKGFGFI PDD  +DLFVHQ+ IRSEGFR+L +G+TVEF ++   D R
Sbjct: 7   QRAKGTVKWFSDQKGFGFITPDDGGEDLFVHQSGIRSEGFRSLAEGETVEFEVESGGDGR 66

Query: 65  AKAVDVTVID----RSTRYGGGRGGGR---------------GRGGSYG----------- 124
            KAVDVT  D    +  R GGG GGGR               GRGGS G           
Sbjct: 67  TKAVDVTGPDGAAVQGGRGGGGGGGGRGGGGYGGGSGGYGGGGRGGSRGYGGGDGGYGGG 126

Query: 125 -------RFGGGGRGFGRGRWNEGPSGGGRGGGE-CYNCGRMGHLARDCYRGNGGAPSGR 177
                  R+GGGG G+G G    G  GGG GGG  C+ CG  GH ARDC +  GG   G 
Sbjct: 127 GGYGGGSRYGGGGGGYGGG---GGYGGGGSGGGSGCFKCGESGHFARDCSQSGGGG-GGG 186

BLAST of Cucsat.G5248 vs. ExPASy Swiss-Prot
Match: Q38896 (Cold shock domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=CSP4 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-34
Identity = 93/182 (51.10%), Postives = 111/182 (60.99%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           R  GTV+WF  QKGFGFI P D  DDLFVHQ+SIRSEGFR+L   ++VEF ++ D   R 
Sbjct: 14  RRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNSGRP 73

Query: 66  KAVDVTVIDRSTRYG--------GGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGR 125
           KA++V+  D +   G        GGRGG  G GG  G  GGG  G G G    G  GGG 
Sbjct: 74  KAIEVSGPDGAPVQGNSGGGGSSGGRGGFGGGGGRGGGRGGGSYGGGYGGRGSGGRGGGG 133

Query: 126 GGGECYNCGRMGHLARDCYRGNG---GAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 177
           G   C+ CG  GH+AR+C +G G   G   G RY  G G GGGGGG  CY+CG++GH AR
Sbjct: 134 GDNSCFKCGEPGHMARECSQGGGGYSGGGGGGRYGSGGGGGGGGGGLSCYSCGESGHFAR 193

BLAST of Cucsat.G5248 vs. ExPASy Swiss-Prot
Match: Q94C69 (Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.2e-26
Identity = 86/189 (45.50%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RS G V WFS  KG+GFI PDD  ++LFVHQ+SI S+GFR+L  G++VE+ I    D + 
Sbjct: 10  RSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIALGSDGKT 69

Query: 66  KAVDVTV-----IDRSTRYGGGRGGGRGRGGSYGRF------GGGGRGFGRGRWNEGPSG 125
           KA++VT      +++      G GG     G  G        G GG+ FG         G
Sbjct: 70  KAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFG--------GG 129

Query: 126 GGR---GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGH 181
           GGR   G GECY CG +GH ARDC R +GG  SG         GGGGGGR CY+CG+ GH
Sbjct: 130 GGRRSGGEGECYMCGDVGHFARDC-RQSGGGNSG---------GGGGGGRPCYSCGEVGH 180

BLAST of Cucsat.G5248 vs. ExPASy Swiss-Prot
Match: O65639 (Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.7e-24
Identity = 82/183 (44.81%), Postives = 98/183 (53.55%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RSTG V WF+A KG+GFI PDD + +LFVHQ+SI SEG+R+L  G  VEF I    D + 
Sbjct: 10  RSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQGSDGKT 69

Query: 66  KAVDVTV-------IDRSTRYGGGRGGGRGRG-GSYGRFGGGGRGFGRGRWNEGPSGGGR 125
           KAV+VT         + ++R  G R GG G G  + G  G   +  G G    G     R
Sbjct: 70  KAVNVTAPGGGSLKKENNSRGNGARRGGGGSGCYNCGELGHISKDCGIGGGGGGGERRSR 129

Query: 126 GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQ 181
           GG  CYNCG  GH ARDC     G   G            GG  GCY CGD GH+ARDC 
Sbjct: 130 GGEGCYNCGDTGHFARDCTSAGNGDQRG---------ATKGGNDGCYTCGDVGHVARDCT 183

BLAST of Cucsat.G5248 vs. NCBI nr
Match: XP_004140332.1 (cold shock protein 2 [Cucumis sativus] >XP_011655173.1 cold shock protein 2 [Cucumis sativus])

HSP 1 Score: 358 bits (918), Expect = 1.80e-124
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120

Query: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180
           ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES
Sbjct: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180

BLAST of Cucsat.G5248 vs. NCBI nr
Match: XP_008465791.1 (PREDICTED: cold shock protein 2-like [Cucumis melo] >XP_008465792.1 PREDICTED: cold shock protein 2-like [Cucumis melo] >KAA0047448.1 cold shock protein 2-like [Cucumis melo var. makuwa] >TYJ98642.1 cold shock protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 340 bits (871), Expect = 2.74e-117
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGG-RGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GPSGGG RGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

BLAST of Cucsat.G5248 vs. NCBI nr
Match: KGN51220.2 (hypothetical protein Csa_008318 [Cucumis sativus])

HSP 1 Score: 349 bits (896), Expect = 1.61e-116
Identity = 176/177 (99.44%), Postives = 177/177 (100.00%), Query Frame = 0

Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR
Sbjct: 322 RRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 381

Query: 65  AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGGECYN 124
           AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGGECYN
Sbjct: 382 AKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGGECYN 441

Query: 125 CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK 181
           CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK
Sbjct: 442 CGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNESK 498

BLAST of Cucsat.G5248 vs. NCBI nr
Match: XP_038906549.1 (glycine-rich protein 2-like [Benincasa hispida])

HSP 1 Score: 300 bits (769), Expect = 1.11e-101
Identity = 160/187 (85.56%), Postives = 169/187 (90.37%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA++DLFVHQTSIRSEGFRTLFDGQ VEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASEDLFVHQTSIRSEGFRTLFDGQAVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRG-RWNE-----GPSG 120
           +DQR+KAVDVTVI RST YGGGRGGGRGRGG+YGRFGGGGRGFGRG R+ +     G  G
Sbjct: 61  EDQRSKAVDVTVIGRSTGYGGGRGGGRGRGGAYGRFGGGGRGFGRGGRYEDSSGGFGGRG 120

Query: 121 GGRGGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 180
           GGRGGGECYNCGRMGHLARDCYRGNGGAP GR Y GGRGYGGG GG GCYNCGDTGH AR
Sbjct: 121 GGRGGGECYNCGRMGHLARDCYRGNGGAPPGRGYGGGRGYGGGAGG-GCYNCGDTGHFAR 180

BLAST of Cucsat.G5248 vs. NCBI nr
Match: XP_022993723.1 (cold shock protein 2-like [Cucurbita maxima] >KAG6578982.1 Cold shock protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016506.1 Cold shock protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293 bits (751), Expect = 8.66e-99
Identity = 161/196 (82.14%), Postives = 167/196 (85.20%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPSGG 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GPSGG
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ------GRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGYGGGGGGRGCYN 180
                 GRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGYGGGGGGRGCYN
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGRGCYN 180

BLAST of Cucsat.G5248 vs. ExPASy TrEMBL
Match: A0A0A0KRF7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G374720 PE=4 SV=1)

HSP 1 Score: 358 bits (918), Expect = 8.72e-125
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGRGGG 120

Query: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180
           ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES
Sbjct: 121 ECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNES 180

BLAST of Cucsat.G5248 vs. ExPASy TrEMBL
Match: A0A1S3CPN9 (cold shock protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503390 PE=4 SV=1)

HSP 1 Score: 340 bits (871), Expect = 1.33e-117
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGG-RGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GPSGGG RGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

BLAST of Cucsat.G5248 vs. ExPASy TrEMBL
Match: A0A5A7TX13 (Cold shock protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold507G00260 PE=4 SV=1)

HSP 1 Score: 340 bits (871), Expect = 1.33e-117
Identity = 173/182 (95.05%), Postives = 178/182 (97.80%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDA+DDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDASDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGG-RGG 120
           QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRW +GPSGGG RGG
Sbjct: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWYDGPSGGGGRGG 120

Query: 121 GECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQNE 180
           GECYNCGRMGHLARDCYRGNGGAP GRRY+GGRGYGGGGGGRGCYNCGDTGH ARDCQNE
Sbjct: 121 GECYNCGRMGHLARDCYRGNGGAPPGRRYEGGRGYGGGGGGRGCYNCGDTGHFARDCQNE 180

BLAST of Cucsat.G5248 vs. ExPASy TrEMBL
Match: A0A6J1K351 (cold shock protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489638 PE=4 SV=1)

HSP 1 Score: 293 bits (751), Expect = 4.19e-99
Identity = 161/196 (82.14%), Postives = 167/196 (85.20%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPSGG 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GPSGG
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ------GRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGYGGGGGGRGCYN 180
                 GRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGYGGGGGGRGCYN
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGRGCYN 180

BLAST of Cucsat.G5248 vs. ExPASy TrEMBL
Match: A0A6J1FIQ9 (cold shock protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444649 PE=4 SV=1)

HSP 1 Score: 288 bits (738), Expect = 4.29e-97
Identity = 161/198 (81.31%), Postives = 167/198 (84.34%), Query Frame = 0

Query: 1   MAELKRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYD 60
           MAE+KRS+GTVRWFSAQKGFGFIAPDDATDDLFVHQTSI+SEGFRTLFDGQ VEF IDYD
Sbjct: 1   MAEVKRSSGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIQSEGFRTLFDGQAVEFAIDYD 60

Query: 61  QDQRAKAVDVTVIDRSTRYGGGRGGGRGRGGSYGRFGG----GGRGFGRGRWN-EGPSGG 120
           +DQRAKAVDVTV  RS+ YGGGRGG RGRGG YGRFGG    GGRGFGRG    +GPSGG
Sbjct: 61  EDQRAKAVDVTVTGRSSGYGGGRGGARGRGGGYGRFGGEGGYGGRGFGRGGGRYDGPSGG 120

Query: 121 ------GRGGGECYNCGRMGHLARDCYRGNGGAP----SGRRYDGGRGYGGGGGG--RGC 180
                 GRGGGECYNCGRMGHLARDCYRGNGGAP     GR Y GGRGYGGGGGG  RGC
Sbjct: 121 FGGGGGGRGGGECYNCGRMGHLARDCYRGNGGAPPSRGGGRGYGGGRGYGGGGGGGGRGC 180

BLAST of Cucsat.G5248 vs. TAIR 10
Match: AT4G38680.1 (glycine rich protein 2 )

HSP 1 Score: 163.3 bits (412), Expect = 1.8e-40
Identity = 102/199 (51.26%), Postives = 121/199 (60.80%), Query Frame = 0

Query: 5   KRSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQR 64
           +R  G+V+WF  QKGFGFI PDD  DDLFVHQ+SIRSEGFR+L   + VEF ++ D + R
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNNNR 68

Query: 65  AKAVDVTVIDRST--------------RYGGGRGGGRGRGGSYGRFGGGGRGFGRGRWNE 124
            KA+DV+  D +                +GGGRGGGRG GG Y   GGGG G+G      
Sbjct: 69  PKAIDVSGPDGAPVQGNSGGGSSGGRGGFGGGRGGGRGSGGGY---GGGGGGYG------ 128

Query: 125 GPSGGGRGGGECYNCGRMGHLARDCYRGNGG-APSGRRYDGGRGYGGGGGGRG------- 177
           G  GGGRGG +CY CG  GH+ARDC  G GG    G  Y GG GYGGGGGG G       
Sbjct: 129 GRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYGGGGGYGGGGGGYGGGGRGGG 188

BLAST of Cucsat.G5248 vs. TAIR 10
Match: AT2G21060.1 (glycine-rich protein 2B )

HSP 1 Score: 147.1 bits (370), Expect = 1.3e-35
Identity = 93/182 (51.10%), Postives = 111/182 (60.99%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           R  GTV+WF  QKGFGFI P D  DDLFVHQ+SIRSEGFR+L   ++VEF ++ D   R 
Sbjct: 14  RRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNSGRP 73

Query: 66  KAVDVTVIDRSTRYG--------GGRGGGRGRGGSYGRFGGGGRGFGRGRWNEGPSGGGR 125
           KA++V+  D +   G        GGRGG  G GG  G  GGG  G G G    G  GGG 
Sbjct: 74  KAIEVSGPDGAPVQGNSGGGGSSGGRGGFGGGGGRGGGRGGGSYGGGYGGRGSGGRGGGG 133

Query: 126 GGGECYNCGRMGHLARDCYRGNG---GAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLAR 177
           G   C+ CG  GH+AR+C +G G   G   G RY  G G GGGGGG  CY+CG++GH AR
Sbjct: 134 GDNSCFKCGEPGHMARECSQGGGGYSGGGGGGRYGSGGGGGGGGGGLSCYSCGESGHFAR 193

BLAST of Cucsat.G5248 vs. TAIR 10
Match: AT2G17870.1 (cold shock domain protein 3 )

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-27
Identity = 86/189 (45.50%), Postives = 108/189 (57.14%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RS G V WFS  KG+GFI PDD  ++LFVHQ+SI S+GFR+L  G++VE+ I    D + 
Sbjct: 10  RSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIALGSDGKT 69

Query: 66  KAVDVTV-----IDRSTRYGGGRGGGRGRGGSYGRF------GGGGRGFGRGRWNEGPSG 125
           KA++VT      +++      G GG     G  G        G GG+ FG         G
Sbjct: 70  KAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFG--------GG 129

Query: 126 GGR---GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGH 181
           GGR   G GECY CG +GH ARDC R +GG  SG         GGGGGGR CY+CG+ GH
Sbjct: 130 GGRRSGGEGECYMCGDVGHFARDC-RQSGGGNSG---------GGGGGGRPCYSCGEVGH 180

BLAST of Cucsat.G5248 vs. TAIR 10
Match: AT4G36020.1 (cold shock domain protein 1 )

HSP 1 Score: 112.1 bits (279), Expect = 4.8e-25
Identity = 82/183 (44.81%), Postives = 98/183 (53.55%), Query Frame = 0

Query: 6   RSTGTVRWFSAQKGFGFIAPDDATDDLFVHQTSIRSEGFRTLFDGQTVEFTIDYDQDQRA 65
           RSTG V WF+A KG+GFI PDD + +LFVHQ+SI SEG+R+L  G  VEF I    D + 
Sbjct: 10  RSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQGSDGKT 69

Query: 66  KAVDVTV-------IDRSTRYGGGRGGGRGRG-GSYGRFGGGGRGFGRGRWNEGPSGGGR 125
           KAV+VT         + ++R  G R GG G G  + G  G   +  G G    G     R
Sbjct: 70  KAVNVTAPGGGSLKKENNSRGNGARRGGGGSGCYNCGELGHISKDCGIGGGGGGGERRSR 129

Query: 126 GGGECYNCGRMGHLARDCYRGNGGAPSGRRYDGGRGYGGGGGGRGCYNCGDTGHLARDCQ 181
           GG  CYNCG  GH ARDC     G   G            GG  GCY CGD GH+ARDC 
Sbjct: 130 GGEGCYNCGDTGHFARDCTSAGNGDQRG---------ATKGGNDGCYTCGDVGHVARDCT 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q411882.5e-3951.26Cold shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=CSP2 PE=1 SV=1[more]
P274841.7e-3550.00Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1[more]
Q388961.9e-3451.10Cold shock domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=CSP4 P... [more]
Q94C694.2e-2645.50Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 P... [more]
O656396.7e-2444.81Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004140332.11.80e-124100.00cold shock protein 2 [Cucumis sativus] >XP_011655173.1 cold shock protein 2 [Cuc... [more]
XP_008465791.12.74e-11795.05PREDICTED: cold shock protein 2-like [Cucumis melo] >XP_008465792.1 PREDICTED: c... [more]
KGN51220.21.61e-11699.44hypothetical protein Csa_008318 [Cucumis sativus][more]
XP_038906549.11.11e-10185.56glycine-rich protein 2-like [Benincasa hispida][more]
XP_022993723.18.66e-9982.14cold shock protein 2-like [Cucurbita maxima] >KAG6578982.1 Cold shock protein 1,... [more]
Match NameE-valueIdentityDescription
A0A0A0KRF78.72e-125100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G374720 PE=4 SV=1[more]
A0A1S3CPN91.33e-11795.05cold shock protein 2-like OS=Cucumis melo OX=3656 GN=LOC103503390 PE=4 SV=1[more]
A0A5A7TX131.33e-11795.05Cold shock protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1K3514.19e-9982.14cold shock protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111489638 PE=4 SV=1[more]
A0A6J1FIQ94.29e-9781.31cold shock protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444649 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT4G38680.11.8e-4051.26glycine rich protein 2 [more]
AT2G21060.11.3e-3551.10glycine-rich protein 2B [more]
AT2G17870.13.0e-2745.50cold shock domain protein 3 [more]
AT4G36020.14.8e-2544.81cold shock domain protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002059Cold-shock protein, DNA-bindingPRINTSPR00050COLDSHOCKcoord: 30..39
score: 58.54
coord: 45..63
score: 35.07
coord: 9..24
score: 60.88
IPR002059Cold-shock protein, DNA-bindingPFAMPF00313CSDcoord: 8..72
e-value: 1.4E-22
score: 79.3
IPR002059Cold-shock protein, DNA-bindingPROSITEPS51857CSD_2coord: 6..72
score: 30.728745
IPR002059Cold-shock protein, DNA-bindingCDDcd04458CSP_CDScoord: 8..72
e-value: 3.80468E-23
score: 85.323
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 121..137
e-value: 2.7E-4
score: 30.2
coord: 162..178
e-value: 1.2E-5
score: 34.7
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 161..177
e-value: 1.2E-7
score: 31.5
coord: 121..135
e-value: 6.2E-7
score: 29.2
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 122..135
score: 11.070239
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 163..178
score: 11.086722
IPR011129Cold shock domainSMARTSM00357csp_8coord: 8..73
e-value: 1.1E-22
score: 91.4
NoneNo IPR availableGENE3D2.40.50.140coord: 1..71
e-value: 1.5E-24
score: 87.6
NoneNo IPR availableGENE3D4.10.60.10coord: 114..152
e-value: 1.0E-6
score: 30.3
coord: 158..181
e-value: 2.3E-6
score: 29.1
NoneNo IPR availablePANTHERPTHR46565COLD SHOCK DOMAIN PROTEIN 2coord: 1..179
IPR019844Cold-shock (CSD) domainPROSITEPS00352CSD_1coord: 20..39
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 112..139
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 144..178
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 5..73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G5248.T2Cucsat.G5248.T2mRNA
Cucsat.G5248.T1Cucsat.G5248.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding