Cucsat.G520 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G520
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Aquaporin NIP1.1, putative
Location	ctg1: 10181046 .. 10183038 (-)
RNA-Seq Expression	Cucsat.G520
Synteny	Cucsat.G520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGAACAAAACATTACGTACGCATGAATGATAGGAAGTAGACTGTGGTTAGCTACACATCAAAACAACTTTACCAAATCACCATAATTTTAACTACAAAACGCACACCAACTTTAAACTCACGTACCATCAAACTCATGCTTTTCAAACTTTTTAACCACCCTTCAACAACCACATTACACTGTACCAACTCCACATATGGCTTCAAATAATAATAATAATACAATCCCTGTCACCGTCTCCGCCACCTTCCCTCAACCGACGACAATGGACCATAATCTAGTTCGCCCAGTAATGCTTCGAATACTATTTTTCTATTGCAACGATGGTCGACCGCTGCTATGGCCTTAATATGTTTTTGGTGTAGGTTTTGGGAGAGATGGTGGGGAGTTTTTTGTTGATTCTTTGCGTGAGCGGGGTAACGGCGACCGGGCAGCTGACGGGCAGTCAGATGGGTATACTGGACTATGCCGTGGCTGCTGGTTTGACGGTGGGCGTTTTGACGTTCTGCTTTGCTCCCATTTCTGGTGCTCATTTTAATCCAGCCATCACTCTTGCCTCTGCCATTTCTGGTCACTTTCCATGGTCTAGGGTACTACTATATACTCTTCTTTTTTAATTTTCAAATTAATTAAAATTTACTTTTATTTCATTAAATTATGGGATTCAAAGTTTTTGTTCTTTTATTTAAAATAACATAAAACTAAGTTGAAAGTTTTTAATGTTTGGAGATTAGGTAATGGCATATGTGGTGGCTCAAACGACAGGTTGTGTAATGGCAACGTATGCAGCAATGTTTGTTTTTGGCATAAAACCACAACAGTTGATCACTCGGCCACTTTATAATTATTCTTCTCCCTTTTCTGCCTTCTTCCTCGAACTTCTTTTAACTTTCATTCTCATGTTCCTCCTTTCTTCTTTATCCCATCAATCCCAATTAGTAAGTCGTTTACTTATAATCAAACTTTCATTTTTGTGTACTCTTAATTATTATATATAAGACTATAAGTGATGATCACCTTAACCCATCCAACATATTATTTTTCTCTTTAATCATGTATAGGTTCGTCAATTTTCTGGCTTTGTCATTGGAATGGCCATTGCTCTTGCTGTGTTTATTGCCGGGTACGTCTTGGCTACATTTTCGATCTTCTACTTATCAAAGCAAATAAATGTTATATTGTCGATCATTCTCTCATTCTTAGTGTTTGGTGTTTGGTTTCTCTACATTTTAAAATAATTCACTTTTACTTCTAAATTTTTACTATAATTTTTATTTCATCTTTTAGGTTTTAACCTATTAAAACGAGTTTAGTTTTCATTTAGTCTACAATTTTACCTATTCTTAGTTGTTCACTCCTATTTGACGTGATGAAGAATTAACGAAAGCTAATTTATTTAATTATGTTAAGCTGGTTAATATATATTAACACCAAACTGCTGAAAAAGATAGTTGTTAAAAACAAAAGTAAATTTTAGAAACCAAATTAAAATAAAACTCATGAATAAAAAAGGTAACTGATTATTTATTTAAAAAAAAAAAGAGGCTGACGGGTTTTGGGCCAATGCAGGCCCATTTCCGGTGCATCAATGAACCCAGCAAGATCTTTAGGACCGGCAATTGTTTCATGGGCTTTCGATGACATATGGATCTATATTACGGCTCCGGCCATCGGGGCCATCACCGGGGCGTTCATCAGCGACTTCCTCCGCCTTAGTCCTCCACCGCCTCAACCATCCAACGCCAAATATTTTGATGTCTCCTCCTCTGCCAACGCATATCTAATTACTTAGCACAAATCATTGCTCTTCATCATCCCTCTATATCTCCAGTTGCTTTTTCTATGTTAAAATGATCTATCATCTTATATATACGTGGAATAACAATTTTAGTTATAAAATCATGTCATTTCCTTACGTGTAAATATGTTGGTTTTTCTTTCTAATTTCAAGAAAATGTATATCCATCTTCGTCTAGATCAATAACATTATTTGAGATAATTATTTTTAAAAAATTATTTGAGA

Coding sequence (CDS)

ATGGCTTCAAATAATAATAATAATACAATCCCTGTCACCGTCTCCGCCACCTTCCCTCAACCGACGACAATGGACCATAATCTAGTTCGCCCAGTTTTGGGAGAGATGGTGGGGAGTTTTTTGTTGATTCTTTGCGTGAGCGGGGTAACGGCGACCGGGCAGCTGACGGGCAGTCAGATGGGTATACTGGACTATGCCGTGGCTGCTGGTTTGACGGTGGGCGTTTTGACGTTCTGCTTTGCTCCCATTTCTGGTGCTCATTTTAATCCAGCCATCACTCTTGCCTCTGCCATTTCTGGTCACTTTCCATGGTCTAGGGTAATGGCATATGTGGTGGCTCAAACGACAGGTTGTGTAATGGCAACGTATGCAGCAATGTTTGTTTTTGGCATAAAACCACAACAGTTGATCACTCGGCCACTTTATAATTATTCTTCTCCCTTTTCTGCCTTCTTCCTCGAACTTCTTTTAACTTTCATTCTCATGTTCCTCCTTTCTTCTTTATCCCATCAATCCCAATTAGTTCGTCAATTTTCTGGCTTTGTCATTGGAATGGCCATTGCTCTTGCTGTGTTTATTGCCGGGCCCATTTCCGGTGCATCAATGAACCCAGCAAGATCTTTAGGACCGGCAATTGTTTCATGGGCTTTCGATGACATATGGATCTATATTACGGCTCCGGCCATCGGGGCCATCACCGGGGCGTTCATCAGCGACTTCCTCCGCCTTAGTCCTCCACCGCCTCAACCATCCAACGCCAAATATTTTGATGTCTCCTCCTCTGCCAACGCATATCTAATTACTTAG

Protein sequence

MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVRQFSGFVIGMAIALAVFIAGPISGASMNPARSLGPAIVSWAFDDIWIYITAPAIGAITGAFISDFLRLSPPPPQPSNAKYFDVSSSANAYLIT

Homology

[Close]

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot
Match: Q8LAI1 (Probable aquaporin NIP7-1 OS=Arabidopsis thaliana OX=3702 GN=NIP7-1 PE=2 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 7.7e-26
Identity = 67/148 (45.27%), Postives = 98/148 (66.22%), Query Frame = 0


Query: 21  PTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCF 80
           P  +D N +R V+ E+VG+F+L+  V GV ++ QL+G  +G+L+YAV AGL+V V+ +  
Sbjct: 37  PYDIDLNPLRIVMAELVGTFILMFSVCGVISSTQLSGGHVGLLEYAVTAGLSVVVVVYSI 96

Query: 81  APISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLITRP 140
             ISGAH NP+IT+A A+ G FPWS+V  Y+ AQT G   AT   + V+G+    + T+P
Sbjct: 97  GHISGAHLNPSITIAFAVFGGFPWSQVPLYITAQTLGATAATLVGVSVYGVNADIMATKP 156

Query: 141 LYNYSSPFSAFFLELLLTFILMFLLSSL 169
                S  SAFF+EL+ T I++FL S+L
Sbjct: 157 AL---SCVSAFFVELIATSIVVFLASAL 181

[Close]

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot
Match: Q0DK16 (Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.0e-14
Identity = 52/148 (35.14%), Postives = 76/148 (51.35%), Query Frame = 0


Query: 32  VLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPA 91
           V+ E++G+F LI       A  + TG  +      +  GL V V+ +    ISGAH NPA
Sbjct: 56  VIAEILGTFFLIFAGCAAVAVNKRTGGTVTFPGICITWGLAVMVMVYSVGHISGAHLNPA 115

Query: 92  ITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLI-TRPLYNYSSPFSA 151
           +TLA A  G FPW RV AY  AQ  G   A+ A   +FG  P+    T P     S   +
Sbjct: 116 VTLAFATCGRFPWRRVPAYAAAQVAGSAAASAALRALFGGAPEHFFGTAPA---GSDVQS 175

Query: 152 FFLELLLTFILMFLLSSLSHQSQLVSRL 179
             +E ++TF LMF++S ++  ++ +  L
Sbjct: 176 LAMEFIITFYLMFVVSGVATDNRAIGEL 200

[Close]

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot
Match: Q9FIZ9 (Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 51/167 (30.54%), Postives = 87/167 (52.10%), Query Frame = 0


Query: 28  LVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAH 87
           L + ++ EM+G++ ++    GV     L G  +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 88  FNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLI-TRPLYNYSS 147
           FNPA+T+  AI   FPW +V  Y+ AQ  G ++A+     +F + P+    T P     S
Sbjct: 101 FNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPA---DS 160

Query: 148 PFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYYI 194
           P  A   E++++F+LMF++S ++  ++ V  L  I +     +N ++
Sbjct: 161 PARALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFV 204

[Close]

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot
Match: Q8W036 (Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.2e-14
Identity = 52/167 (31.14%), Postives = 86/167 (51.50%), Query Frame = 0


Query: 28  LVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAH 87
           L + ++ EM+G++ +I    GV     L G  +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 88  FNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLI-TRPLYNYSS 147
           FNPA+T+  A+   FPW +V  Y+ AQ TG ++A+     +F + P+    T P     S
Sbjct: 101 FNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPT---DS 160

Query: 148 PFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYYI 194
              A   E++++F+LMF++S ++  S+    L  I +     LN ++
Sbjct: 161 SGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFV 204

[Close]

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.7e-14
Identity = 58/181 (32.04%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 13  TVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLT 72
           T    FP P   D +L R +  E VG+F+LI   +      Q       ++  A  AGL 
Sbjct: 64  TCFTDFPSP---DVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLA 123

Query: 73  VGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIK 132
           V ++      ISGAH NP++T+A A   HFPW+ V AY+ AQ +  + A++A   VF   
Sbjct: 124 VMIIILSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPF 183

Query: 133 PQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYY 192
               +T P  +      AF LE ++TFIL+F++++++  ++ V  L  I +     LN  
Sbjct: 184 MSGGVTIPSVSLG---QAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNIL 238

Query: 193 I 194
           +
Sbjct: 244 V 238

[Close]

BLAST of Cucsat.G520 vs. NCBI nr
Match: XP_004134455.2 (probable aquaporin NIP7-1 [Cucumis sativus] >KGN57016.1 hypothetical protein Csa_011741 [Cucumis sativus])

HSP 1 Score: 338 bits (866), Expect = 5.19e-115
Identity = 175/177 (98.87%), Postives = 176/177 (99.44%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60
           MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM
Sbjct: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60

Query: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120
           GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM
Sbjct: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120

Query: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVSR 177
           ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLV +
Sbjct: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVRQ 177

[Close]

BLAST of Cucsat.G520 vs. NCBI nr
Match: XP_008438714.1 (PREDICTED: probable aquaporin NIP7-1 [Cucumis melo])

HSP 1 Score: 322 bits (825), Expect = 9.01e-109
Identity = 165/173 (95.38%), Postives = 171/173 (98.84%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60
           MASNNNNNTIPVTVSATFP+PTTMD NLVRPVLGEMVGSFLLI+CVSGVTATGQLTGSQM
Sbjct: 1   MASNNNNNTIPVTVSATFPRPTTMDDNLVRPVLGEMVGSFLLIVCVSGVTATGQLTGSQM 60

Query: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120
           G+LDYA+AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWSRVMAYVVAQTTGCVM
Sbjct: 61  GVLDYAIAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMAYVVAQTTGCVM 120

Query: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           ATYAAMFVFGIKPQQLITRPL+NYSSPFSAFFLELLLTFILMFLLSSLS+QSQ
Sbjct: 121 ATYAAMFVFGIKPQQLITRPLHNYSSPFSAFFLELLLTFILMFLLSSLSYQSQ 173

[Close]

BLAST of Cucsat.G520 vs. NCBI nr
Match: XP_022979516.1 (probable aquaporin NIP7-1 [Cucurbita maxima])

HSP 1 Score: 241 bits (616), Expect = 5.69e-77
Identity = 130/178 (73.03%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSAT-----FPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQL 60
           MASNN N  IP  VS        PQPT MDHN  RPVLGEM+G+FLL++CVSGV ATGQ+
Sbjct: 1   MASNNTN-PIPTAVSGNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQM 60

Query: 61  TGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQT 120
            G Q+G+LDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWS VM Y VAQT
Sbjct: 61  MGGQVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQT 120

Query: 121 TGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           TGCVMATYA MFV+ IKPQQL TRP   +    SAFF+ELL TFILMFL+SSL HQS+
Sbjct: 121 TGCVMATYAGMFVYDIKPQQLTTRP---FRGSMSAFFVELLATFILMFLISSLPHQSR 174

[Close]

BLAST of Cucsat.G520 vs. NCBI nr
Match: XP_022956061.1 (probable aquaporin NIP7-1 [Cucurbita moschata])

HSP 1 Score: 241 bits (614), Expect = 1.14e-76
Identity = 130/178 (73.03%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSAT-----FPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQL 60
           MASNN N  IP  VS        PQPT MDHN  RPVLGEM+G+FLL++CVSGV ATGQ+
Sbjct: 1   MASNNTN-PIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQM 60

Query: 61  TGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQT 120
            G Q+G+LDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWS VM Y VAQT
Sbjct: 61  MGGQVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQT 120

Query: 121 TGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           TGCVMATYA MFV+ IKPQQL TRP   +    SAFF+ELL TFILMFL+SSL HQS+
Sbjct: 121 TGCVMATYAGMFVYDIKPQQLTTRP---FRGSMSAFFVELLATFILMFLISSLPHQSR 174

[Close]

BLAST of Cucsat.G520 vs. NCBI nr
Match: KAG7018680.1 (putative aquaporin NIP7-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241 bits (614), Expect = 1.14e-76
Identity = 130/178 (73.03%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSAT-----FPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQL 60
           MASNN N  IP  VS        PQPT MDHN  RPVLGEM+G+FLL++CVSGV ATGQ+
Sbjct: 1   MASNNTN-PIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQM 60

Query: 61  TGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQT 120
            G Q+G+LDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWS VM Y VAQT
Sbjct: 61  MGGQVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQT 120

Query: 121 TGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           TGCVMATYA MFV+ IKPQQL TRP   +    SAFF+ELL TFILMFL+SSL HQS+
Sbjct: 121 TGCVMATYAGMFVYDIKPQQLTTRP---FRGSMSAFFVELLATFILMFLISSLPHQSR 174

[Close]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL
Match: A0A0A0LAF0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149960 PE=3 SV=1)

HSP 1 Score: 338 bits (866), Expect = 2.51e-115
Identity = 175/177 (98.87%), Postives = 176/177 (99.44%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60
           MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM
Sbjct: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60

Query: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120
           GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM
Sbjct: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120

Query: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVSR 177
           ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLV +
Sbjct: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVRQ 177

[Close]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL
Match: A0A1S3AX29 (probable aquaporin NIP7-1 OS=Cucumis melo OX=3656 GN=LOC103483738 PE=3 SV=1)

HSP 1 Score: 322 bits (825), Expect = 4.36e-109
Identity = 165/173 (95.38%), Postives = 171/173 (98.84%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60
           MASNNNNNTIPVTVSATFP+PTTMD NLVRPVLGEMVGSFLLI+CVSGVTATGQLTGSQM
Sbjct: 1   MASNNNNNTIPVTVSATFPRPTTMDDNLVRPVLGEMVGSFLLIVCVSGVTATGQLTGSQM 60

Query: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120
           G+LDYA+AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWSRVMAYVVAQTTGCVM
Sbjct: 61  GVLDYAIAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVMAYVVAQTTGCVM 120

Query: 121 ATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           ATYAAMFVFGIKPQQLITRPL+NYSSPFSAFFLELLLTFILMFLLSSLS+QSQ
Sbjct: 121 ATYAAMFVFGIKPQQLITRPLHNYSSPFSAFFLELLLTFILMFLLSSLSYQSQ 173

[Close]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL
Match: A0A6J1IR00 (probable aquaporin NIP7-1 OS=Cucurbita maxima OX=3661 GN=LOC111479196 PE=3 SV=1)

HSP 1 Score: 241 bits (616), Expect = 2.75e-77
Identity = 130/178 (73.03%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSAT-----FPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQL 60
           MASNN N  IP  VS        PQPT MDHN  RPVLGEM+G+FLL++CVSGV ATGQ+
Sbjct: 1   MASNNTN-PIPTAVSGNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQM 60

Query: 61  TGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQT 120
            G Q+G+LDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWS VM Y VAQT
Sbjct: 61  MGGQVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQT 120

Query: 121 TGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           TGCVMATYA MFV+ IKPQQL TRP   +    SAFF+ELL TFILMFL+SSL HQS+
Sbjct: 121 TGCVMATYAGMFVYDIKPQQLTTRP---FRGSMSAFFVELLATFILMFLISSLPHQSR 174

[Close]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL
Match: A0A6J1GVJ7 (probable aquaporin NIP7-1 OS=Cucurbita moschata OX=3662 GN=LOC111457868 PE=3 SV=1)

HSP 1 Score: 241 bits (614), Expect = 5.54e-77
Identity = 130/178 (73.03%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSAT-----FPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQL 60
           MASNN N  IP  VS        PQPT MDHN  RPVLGEM+G+FLL++CVSGV ATGQ+
Sbjct: 1   MASNNTN-PIPTAVSDNNGLLCSPQPTIMDHNPARPVLGEMMGTFLLMICVSGVAATGQM 60

Query: 61  TGSQMGILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQT 120
            G Q+G+LDYA AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWS VM Y VAQT
Sbjct: 61  MGGQVGLLDYAAAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSWVMPYTVAQT 120

Query: 121 TGCVMATYAAMFVFGIKPQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQ 173
           TGCVMATYA MFV+ IKPQQL TRP   +    SAFF+ELL TFILMFL+SSL HQS+
Sbjct: 121 TGCVMATYAGMFVYDIKPQQLTTRP---FRGSMSAFFVELLATFILMFLISSLPHQSR 174

[Close]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL
Match: A0A5A7U237 (Putative aquaporin NIP7-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G006350 PE=3 SV=1)

HSP 1 Score: 205 bits (521), Expect = 7.34e-64
Identity = 107/133 (80.45%), Postives = 115/133 (86.47%), Query Frame = 0


Query: 1   MASNNNNNTIPVTVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQM 60
           MASNNNNNTIPVTVSATFP+PTTMD NLVRPVLGEMVGSFLLI+C+SGVTATGQLTGSQM
Sbjct: 1   MASNNNNNTIPVTVSATFPRPTTMDDNLVRPVLGEMVGSFLLIVCLSGVTATGQLTGSQM 60

Query: 61  GILDYAVAAGLTVGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVM 120
           G+LDYA+AAGLTVGVLTFCFAPISGAHFNPAITLASAI GHFPWSRV      Q +G V+
Sbjct: 61  GVLDYAIAAGLTVGVLTFCFAPISGAHFNPAITLASAIFGHFPWSRVR-----QFSGFVI 120

Query: 121 ATYAAMFVFGIKP 133
               A+ VF   P
Sbjct: 121 GMAIALAVFIAGP 128

[Close]

BLAST of Cucsat.G520 vs. TAIR 10
Match: AT3G06100.1 (NOD26-like intrinsic protein 7;1 )

HSP 1 Score: 118.6 bits (296), Expect = 5.4e-27
Identity = 67/148 (45.27%), Postives = 98/148 (66.22%), Query Frame = 0


Query: 21  PTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCF 80
           P  +D N +R V+ E+VG+F+L+  V GV ++ QL+G  +G+L+YAV AGL+V V+ +  
Sbjct: 37  PYDIDLNPLRIVMAELVGTFILMFSVCGVISSTQLSGGHVGLLEYAVTAGLSVVVVVYSI 96

Query: 81  APISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLITRP 140
             ISGAH NP+IT+A A+ G FPWS+V  Y+ AQT G   AT   + V+G+    + T+P
Sbjct: 97  GHISGAHLNPSITIAFAVFGGFPWSQVPLYITAQTLGATAATLVGVSVYGVNADIMATKP 156

Query: 141 LYNYSSPFSAFFLELLLTFILMFLLSSL 169
                S  SAFF+EL+ T I++FL S+L
Sbjct: 157 AL---SCVSAFFVELIATSIVVFLASAL 181

[Close]

BLAST of Cucsat.G520 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-15
Identity = 51/167 (30.54%), Postives = 87/167 (52.10%), Query Frame = 0


Query: 28  LVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAH 87
           L + ++ EM+G++ ++    GV     L G  +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 88  FNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLI-TRPLYNYSS 147
           FNPA+T+  AI   FPW +V  Y+ AQ  G ++A+     +F + P+    T P     S
Sbjct: 101 FNPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPA---DS 160

Query: 148 PFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYYI 194
           P  A   E++++F+LMF++S ++  ++ V  L  I +     +N ++
Sbjct: 161 PARALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFV 204

[Close]

BLAST of Cucsat.G520 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-15
Identity = 52/167 (31.14%), Postives = 86/167 (51.50%), Query Frame = 0


Query: 28  LVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTVGVLTFCFAPISGAH 87
           L + ++ EM+G++ +I    GV     L G  +      V  GL V V+ +    ISGAH
Sbjct: 41  LTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAH 100

Query: 88  FNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKPQQLI-TRPLYNYSS 147
           FNPA+T+  A+   FPW +V  Y+ AQ TG ++A+     +F + P+    T P     S
Sbjct: 101 FNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPT---DS 160

Query: 148 PFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYYI 194
              A   E++++F+LMF++S ++  S+    L  I +     LN ++
Sbjct: 161 SGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFV 204

[Close]

BLAST of Cucsat.G520 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 79.0 bits (193), Expect = 4.8e-15
Identity = 58/181 (32.04%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 13  TVSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLT 72
           T    FP P   D +L R +  E VG+F+LI   +      Q       ++  A  AGL 
Sbjct: 64  TCFTDFPSP---DVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLA 123

Query: 73  VGVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIK 132
           V ++      ISGAH NP++T+A A   HFPW+ V AY+ AQ +  + A++A   VF   
Sbjct: 124 VMIIILSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPF 183

Query: 133 PQQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYY 192
               +T P  +      AF LE ++TFIL+F++++++  ++ V  L  I +     LN  
Sbjct: 184 MSGGVTIPSVSLG---QAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNIL 238

Query: 193 I 194
           +
Sbjct: 244 V 238

[Close]

BLAST of Cucsat.G520 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 75.1 bits (183), Expect = 6.9e-14
Identity = 58/180 (32.22%), Postives = 87/180 (48.33%), Query Frame = 0


Query: 14  VSATFPQPTTMDHNLVRPVLGEMVGSFLLILCVSGVTATGQLTGSQMGILDYAVAAGLTV 73
           V+ + P P     +L R +  E VG+ +LI   +      Q T     ++  A +AGL V
Sbjct: 67  VTCSLPPPNV---SLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAV 126

Query: 74  GVLTFCFAPISGAHFNPAITLASAISGHFPWSRVMAYVVAQTTGCVMATYAAMFVFGIKP 133
            ++      ISGAH NPA+T+A A   HFPW  V  Y+ AQ    V A +A   VF    
Sbjct: 127 MIVILSTGHISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTM 186

Query: 134 QQLITRPLYNYSSPFSAFFLELLLTFILMFLLSSLSHQSQLVSRLLIIKLSFLCTLNYYI 193
              +T P    S    AF LE +++F LMF++++++  ++ V  L  I +     LN  I
Sbjct: 187 SGGVTVPTVGLS---QAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILI 240

The following BLAST results are available for this feature:

BLAST of Cucsat.G520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAI1	7.7e-26	45.27	Probable aquaporin NIP7-1 OS=Arabidopsis thaliana OX=3702 GN=NIP7-1 PE=2 SV=2	[more]
Q0DK16	1.0e-14	35.14	Aquaporin NIP1-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP1-3 PE=3 SV=2	[more]
Q9FIZ9	5.2e-14	30.54	Putative aquaporin NIP4-1 OS=Arabidopsis thaliana OX=3702 GN=NIP4-1 PE=3 SV=1	[more]
Q8W036	5.2e-14	31.14	Probable aquaporin NIP4-2 OS=Arabidopsis thaliana OX=3702 GN=NIP4-2 PE=2 SV=2	[more]
Q9SV84	6.7e-14	32.04	Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1	[more]

BLAST of Cucsat.G520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134455.2	5.19e-115	98.87	probable aquaporin NIP7-1 [Cucumis sativus] >KGN57016.1 hypothetical protein Csa...	[more]
XP_008438714.1	9.01e-109	95.38	PREDICTED: probable aquaporin NIP7-1 [Cucumis melo]	[more]
XP_022979516.1	5.69e-77	73.03	probable aquaporin NIP7-1 [Cucurbita maxima]	[more]
XP_022956061.1	1.14e-76	73.03	probable aquaporin NIP7-1 [Cucurbita moschata]	[more]
KAG7018680.1	1.14e-76	73.03	putative aquaporin NIP7-1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cucsat.G520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAF0	2.51e-115	98.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149960 PE=3 SV=1	[more]
A0A1S3AX29	4.36e-109	95.38	probable aquaporin NIP7-1 OS=Cucumis melo OX=3656 GN=LOC103483738 PE=3 SV=1	[more]
A0A6J1IR00	2.75e-77	73.03	probable aquaporin NIP7-1 OS=Cucurbita maxima OX=3661 GN=LOC111479196 PE=3 SV=1	[more]
A0A6J1GVJ7	5.54e-77	73.03	probable aquaporin NIP7-1 OS=Cucurbita moschata OX=3662 GN=LOC111457868 PE=3 SV=...	[more]
A0A5A7U237	7.34e-64	80.45	Putative aquaporin NIP7-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]

BLAST of Cucsat.G520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06100.1	5.4e-27	45.27	NOD26-like intrinsic protein 7;1	[more]
AT5G37810.1	3.7e-15	30.54	NOD26-like intrinsic protein 4;1	[more]
AT5G37820.1	3.7e-15	31.14	NOD26-like intrinsic protein 4;2	[more]
AT4G10380.1	4.8e-15	32.04	NOD26-like intrinsic protein 5;1	[more]
AT1G80760.1	6.9e-14	32.22	NOD26-like intrinsic protein 6;1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 71..90 score: 27.55 coord: 34..58 score: 45.63 coord: 118..136 score: 25.38
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 2..147 e-value: 2.5E-23 score: 83.0
IPR023271	Aquaporin-like	GENE3D	1.20.1080.10	Glycerol uptake facilitator protein.	coord: 1..157 e-value: 6.5E-31 score: 109.3
IPR023271	Aquaporin-like	SUPERFAMILY	81338	Aquaporin-like	coord: 2..141
None	No IPR available	PANTHER	PTHR45724:SF26	AQUAPORIN NIP7-1-RELATED	coord: 1..143
IPR034294	Aquaporin transporter	PANTHER	PTHR45724	AQUAPORIN NIP2-1	coord: 1..143
IPR022357	Major intrinsic protein, conserved site	PROSITE	PS00221	MIP	coord: 52..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G520.T1	Cucsat.G520.T1	mRNA
Cucsat.G520.T7	Cucsat.G520.T7	mRNA
Cucsat.G520.T6	Cucsat.G520.T6	mRNA
Cucsat.G520.T3	Cucsat.G520.T3	mRNA
Cucsat.G520.T2	Cucsat.G520.T2	mRNA
Cucsat.G520.T8	Cucsat.G520.T8	mRNA
Cucsat.G520.T5	Cucsat.G520.T5	mRNA
Cucsat.G520.T9	Cucsat.G520.T9	mRNA
Cucsat.G520.T4	Cucsat.G520.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015267	channel activity