Cucsat.G497 (gene) Cucumber (B10) v3

Overview
NameCucsat.G497
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Locationctg1: 9781971 .. 9785441 (-)
RNA-Seq ExpressionCucsat.G497
SyntenyCucsat.G497
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAAAATATAAATTTTACGAATTATAAAGAAAAAATCTAAAAAGGAAGGTTGTTTTTTTCTCTCAAAAAACGGCTCAATTGGAAATGAAAACGGTGCGAAGGAAAACAAGAAAAAGGAGGCAATGAGTCGTGGGCATTATGGTCTTTTTGGGTACAATGAATGTGTATATTAGTTCCCTTCGTTTTCACTTGTTCACGCGTTGCCTTTCAGCAATCGAATTTGATTGGGATTTTTTTTTTTCTGAAGCTGAGGCCGAAGGTTGCAGTTGACGATGGCTGTTCTCGACAAGGCAAGTTTTTCTTTGCTGTTCATTACAATCGCTTCTTTCCATATCTTTAATTTGTTCTGTTCTCCTTTTTCATCAAGAGTTTGGTTTCATCTCCGCACCAATTGGCTTTGCATGTTCGGAATTTCTGGGAATTCTTGGATGATTTCTTCTTGAAATTTAATTTCCTATCGATTTACTTGATAATCTTCCGTAGTTTACTCTGCTGTTAATACTTCTAAACATCAAGATGATGCTGCCCTAGACTGAAACGCTTGCATGTTCCTTTTCCTTGAAACGTGGAACTGGTTTTGGTTTAGACTTCTAGTCACCTTCATTTTTATCTACTTTTCCCTGGGGAAAATGCTGAAATTCCGGTTTGGATTTCGGAATTTCAACGCCAGCTTTGGGGCACTGGGGGGTTTGTTTGGGTTGATGTCTTAATTTCTGTCTATTGTATAATTGTCCAGCATTCATGCATGTTCTTCATTTTTCAAAATTTCGGGAAGCCTTAGTAATATTTATGCCTTTGTTCCGGTGTTGATATGAGTTTTCTGTTTTGGCCTCAATGTAATATTTTGTTTTCCAGTTTGCTGTCAGTATGAAATGGATATCTCGTGTATTTTATATTAACGGATGTGCACGATCACATTCGAAATGAAAAAATGCTTTCAAATTCTTTGCAGTCTTCCCTTTTGTAGTTTGTGGTTATAGGTCTGAGCATCATTGAGCTATTGATGAAAATTTAGGTAGGCTTAAATAGCATATAAATAGTGATGACTGATGGAGATCATATGTTCATAGTTTCTCTTTTTGTCTTAAACGGTGTATTTGGCTATGCATGCATTTGATGTTGTCTAATAAATTATGTAATTATTCAGGACTACTCCCCTGAAATTTGTCCATGTTTGACCCAATTGTGAGCCCTCGTGTGTTTGTAACTATCGTTATGATTACAATCTCTTGAAACTTTCGTTTACAGGATCCAAGAGAGGAAGTGATTCAGGCATGGTTTATGGATGACAGTGATGAGGACCAGAGGCTTCCTCATCACCTTGAACCAAAGCAATATGTATCCTTACAGAAACTTGATGGTACGACTTGCTGAGAAATGTGAATAGAATAGCTTACAGTTGTACAATGCTATGTGATTTCAGATAGACTTATATCGTTTGAATTGCTAACGTTTTAGAACTTGGAGTTCTGAGCTGGAGGCTAGATGCTGACATATATGAAACAGATGAGGAATTGAAGAAAATACGCCATGATCGTAATTACTCCTACATGGTTCGTTTCATTTCCTTGTGTGGTTATAACCTTCTATTTTACCTGTATGGAAAAGAACGTGTTCTAGTGCTTGTTAAAATTGTTTTGAAATCAATTTTCCTGAATCTGATTATTTTGTTTTTTTGCTCTCCTCTATGGATGTCTTTCTTTTGCTATTTATAGTTGACTGCAATTTTACTGTCAGGACTTCTGCGAGGTCTGCCCAGAAAAGCTTCCTAATTACAATGAGAAGATTAAAAACTTTTACGAGGAACACCTTCACACTGATGAGGAGATCCGTTATTGTGTGGCTGGAAGTGGTAGGATTAGTTATATTTCTAGACCTTAAGCATTTATTATTTGTGGCTTCTAATTTTCTTTTTCCTTAATATGGGTGGATGAAGGTTACTTTGATGTTAGAGATCTGAATGACAAATGGATTCGCATTTGGGTAAAGAAGGGAGCAATGATTGTCTTGCCTGCTGGAATTTATCATCGCTTTACTCTGGATTCTGACAATTACATCAAGGTAAGCATATACTTTATAGAACTTGCATTCGTACACTCCGAATGCTTAATGATGAGTGAAGACAGTCTTTCTAAGTTCCAACCCTTTCTCACCATTCATTTGCCTTTCTCACTTGTGTGTTCCTTTTAGAATACTGTGTGTAGGTTCATTTTACTCTTCTCATCATCTACTTTGCAATGATTTCCACTGCACACTTTCTCTGTAGAGAGCAAGGATGTGGTTTACTCCATTCAGTACAACAAGAATTTAGTTCATACTTCATATATGTTTGGCTTTTTGACTTGTAAATAACAATGGGGCTAAGATACCAATCTTCCAACCAATAAAAATCTATAATGGTTATATAATAAAGGCCATCCGTGTGGTAGGAACGATAAAGGGAGAACAATTACTAAGTTCCGTTTGGTGACCATTTCGTTTTTTGTTTAAGTTTTTGAAAATTAAGCTTATTTCCTTTCCATGTCTTAATAATATAATTATTTGCATCAGCAAAATTCTAGAAACAAAAACAACTTTTTGAAAGCTACCTCTTTTAGTTTTCAAATTTTGACTTGCTCTTTTAAATCATTGGTAGAATATTAAGAACAAAGGAAAAAAGTTTTGAGGTGAAAGCAGTGTTTATTGGTTTAATTTTAAGAAACAAAATGGTTACCAAAAGAGGCTTAAGTAATTTTGATTTTGGAGCACCATGTAGTGGCATTAGATTCACTATCCCATGCCTTGCGGAGTAGCAGTATACTTTTCCAATCAAAATTTTTGAACAATGTCGTTAATGCAGTCTTATTATCACCAATTCTATTGCTTAATTGCATTTGTATATATTATAACTGCATTAGCAAGAGGAACAAATTTTCTCATGAAGGTCAATTTTGTAGGCTATGCGACTCTTTGTTGGTGATCCTGTCTGGACTCCTCACAACCGTCCCAATGATCATCTTCCAGCAAGGTATTGCAATTTCTTGGTTGGTGCAGTAATGAGCTCTCTATGCTCCGTAGCATGAAGTTGATTCAATTATACATGGCCCTATGAACTTTTTTGTTACTTTGCAGGAAGGAGTATGTCAAAAGTTTTGTGGAGAAGGAAGCTAGTATCCAAGCCGTTAACGCTGCAGCTTGAAACCTGCCTACTAGAACTTCTAAAGTTTGAAATCAAGTCTCGGGGTACCAAAACATGTTACTATTTGTATGGTCTGGTATGCATGTCCTTTCTAGGGTATGTCTGTGGCTTGTCATGCTTTGCTATGCCTTAGCTTATGCAAAAACTCAAGTATTTCTATTCAGTGTCTTTCATGTTGTGTCTCTAAAATAATGTAATTTTAGCGTATGGTATAGATGAAATAAACATTATACTTATCAGCCGTCATTGTGCATATGTTAACCGACTTTCATATTGCCGAGTTTCTCACAAATTTTATGGAATAAATTGTACTGTGGTGGTATTGTAGGAAATTAAATTCAGAGAAAGTGCAGCATCTTGTGAGTTGAAGTTAATATGTTCGATGTGTATGAATTCTCACCCAAAATTACAAATTCACGTTTTCTAGAAACCCTTCAATTTAGAGAGTAGGTAGATGAAGTTTACATTTTCAAAGA

Coding sequence (CDS)

ATGGCTGTTCTCGACAAGGATCCAAGAGAGGAAGTGATTCAGGCATGGTTTATGGATGACAGTGATGAGGACCAGAGGCTTCCTCATCACCTTGAACCAAAGCAATATGTATCCTTACAGAAACTTGATGAACTTGGAGTTCTGAGCTGGAGGCTAGATGCTGACATATATGAAACAGATGAGGAATTGAAGAAAATACGCCATGATCGTAATTACTCCTACATGGACTTCTGCGAGGTCTGCCCAGAAAAGCTTCCTAATTACAATGAGAAGATTAAAAACTTTTACGAGGAACACCTTCACACTGATGAGGAGATCCGTTATTGTGTGGCTGGAAGTGGTTACTTTGATGTTAGAGATCTGAATGACAAATGGATTCGCATTTGGGTAAAGAAGGGAGCAATGATTGTCTTGCCTGCTGGAATTTATCATCGCTTTACTCTGGATTCTGACAATTACATCAAGGCTATGCGACTCTTTGTTGGTGATCCTGTCTGGACTCCTCACAACCGTCCCAATGATCATCTTCCAGCAAGGAAGGAGTATGTCAAAAGTTTTGTGGAGAAGGAAGCTAGTATCCAAGCCGTTAACGCTGCAGCTTGA

Protein sequence

MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSGYFDVRDLNDKWIRIWVKKGAMIVLPAGIYHRFTLDSDNYIKAMRLFVGDPVWTPHNRPNDHLPARKEYVKSFVEKEASIQAVNAAA
Homology
[Close]
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match: F6HDT7 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Vitis vinifera OX=29760 GN=VIT_05s0020g04080 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 4.4e-51
Identity = 92/109 (84.40%), Postives = 100/109 (91.74%), Query Frame = 0 

Query: 6   KDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKK 65
           KD REEV+QAW+MDDSDEDQRLPHH +PK++VSL +L +LGVLSWRLDAD YETDEELKK
Sbjct: 5   KDDREEVLQAWYMDDSDEDQRLPHHRDPKEFVSLDQLAKLGVLSWRLDADNYETDEELKK 64

Query: 66  IRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           IR  R YSYMDFCEVCPEKLPNY EKIKNF+EEHLHTDEEIRYCVAGSG
Sbjct: 65  IREARGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSG 113

[Close]
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match: Q8W108 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=ARD3 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 8.2e-50
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           M  + KD REEVIQAW+MDDS+EDQRLPHH +PK+++SL KL ELGVLSWRLDAD YETD
Sbjct: 1   MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           E+LKKIR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match: Q8GXE2 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=ARD2 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.1e-49
Identity = 89/109 (81.65%), Postives = 99/109 (90.83%), Query Frame = 0 

Query: 6   KDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKK 65
           KD REEVIQAW+MDDS+EDQRLPHH +PK++VSL KL ELGVLSWRLDAD YETDE+LKK
Sbjct: 6   KDGREEVIQAWYMDDSEEDQRLPHHKDPKEFVSLDKLAELGVLSWRLDADNYETDEDLKK 65

Query: 66  IRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           IR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAG+G
Sbjct: 66  IRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGTG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match: O48707 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=ARD1 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 7.2e-46
Identity = 82/109 (75.23%), Postives = 94/109 (86.24%), Query Frame = 0 

Query: 6   KDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKK 65
           KD  EEVIQAW++D+ +EDQ+LPHH +PK++VSL KL ELGVL WRLDAD YETDEELK+
Sbjct: 6   KDQTEEVIQAWYLDNKEEDQKLPHHKDPKEFVSLDKLAELGVLCWRLDADNYETDEELKR 65

Query: 66  IRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           IR  R YSYMD CEVCPEKLPNY EK+K F+EEHLH DEEIRYC+AGSG
Sbjct: 66  IRESRGYSYMDLCEVCPEKLPNYEEKVKMFFEEHLHIDEEIRYCLAGSG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match: A2XCT8 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. indica OX=39946 GN=ARD2 PE=2 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 4.7e-45
Identity = 79/109 (72.48%), Postives = 94/109 (86.24%), Query Frame = 0 

Query: 6   KDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKK 65
           +D +EEVI+AW+MDDS+EDQRLPHH EPK+++ L KL ELG+LSWRL+AD +E DE LKK
Sbjct: 6   QDGKEEVIEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWENDENLKK 65

Query: 66  IRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           IR  R YSYMD C+VCPEKLPNY  K+KNF+EEHLHTDEEIRYC+ GSG
Sbjct: 66  IREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSG 114

[Close]
BLAST of Cucsat.G497 vs. NCBI nr
Match: XP_004134118.1 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 [Cucumis sativus] >KGN56944.1 hypothetical protein Csa_010565 [Cucumis sativus])

HSP 1 Score: 243 bits (621), Expect = 5.71e-80
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD
Sbjct: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. NCBI nr
Match: XP_008438621.1 (PREDICTED: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Cucumis melo])

HSP 1 Score: 238 bits (608), Expect = 5.45e-78
Identity = 111/114 (97.37%), Postives = 113/114 (99.12%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAVLDKDPREEVIQAW+MDDSDEDQRLPHHLEPKQYVSLQ+LDELGVLSWRLDADIYETD
Sbjct: 1   MAVLDKDPREEVIQAWYMDDSDEDQRLPHHLEPKQYVSLQQLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIRHDRNYSYMDFCEVCPEKLPNY EKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYEEKIKNFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. NCBI nr
Match: AIS71929.1 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 [Citrullus lanatus])

HSP 1 Score: 229 bits (585), Expect = 1.73e-74
Identity = 107/114 (93.86%), Postives = 111/114 (97.37%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAV DKDPREEVIQAW+MDDSDEDQRLPHHLEPKQY+SLQ+LDELGVLSWRLDADIYETD
Sbjct: 1   MAVPDKDPREEVIQAWYMDDSDEDQRLPHHLEPKQYLSLQQLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIR DRNYSYMDFCEVCPEKLPNY EKIK+FYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRRDRNYSYMDFCEVCPEKLPNYEEKIKSFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. NCBI nr
Match: XP_038885145.1 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Benincasa hispida])

HSP 1 Score: 228 bits (582), Expect = 4.95e-74
Identity = 106/114 (92.98%), Postives = 111/114 (97.37%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAV DKDPREEVIQAW+MDDSDEDQRLPHHLEPKQY+SL++LDELGVLSWRLDADIYETD
Sbjct: 1   MAVPDKDPREEVIQAWYMDDSDEDQRLPHHLEPKQYLSLRQLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIR DRNYSYMDFCEVCPEKLPNY EKIKNF+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRRDRNYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. NCBI nr
Match: KAA0049332.1 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Cucumis melo var. makuwa] >TYK17226.1 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 227 bits (579), Expect = 1.56e-73
Identity = 105/108 (97.22%), Postives = 107/108 (99.07%), Query Frame = 0 

Query: 7   DPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKKI 66
           DPREEVIQAW+MDDSDEDQRLPHHLEPKQYVSLQ+LDELGVLSWRLDADIYETDEELKKI
Sbjct: 10  DPREEVIQAWYMDDSDEDQRLPHHLEPKQYVSLQQLDELGVLSWRLDADIYETDEELKKI 69

Query: 67  RHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           RHDRNYSYMDFCEVCPEKLPNY EKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 70  RHDRNYSYMDFCEVCPEKLPNYEEKIKNFYEEHLHTDEEIRYCVAGSG 117

[Close]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match: A0A0A0L7D7 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G146300 PE=3 SV=1)

HSP 1 Score: 243 bits (621), Expect = 2.76e-80
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD
Sbjct: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match: A0A1S3AXH8 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483677 PE=3 SV=1)

HSP 1 Score: 238 bits (608), Expect = 2.64e-78
Identity = 111/114 (97.37%), Postives = 113/114 (99.12%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAVLDKDPREEVIQAW+MDDSDEDQRLPHHLEPKQYVSLQ+LDELGVLSWRLDADIYETD
Sbjct: 1   MAVLDKDPREEVIQAWYMDDSDEDQRLPHHLEPKQYVSLQQLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIRHDRNYSYMDFCEVCPEKLPNY EKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYEEKIKNFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match: A0A097BTZ9 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 229 bits (585), Expect = 8.37e-75
Identity = 107/114 (93.86%), Postives = 111/114 (97.37%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAV DKDPREEVIQAW+MDDSDEDQRLPHHLEPKQY+SLQ+LDELGVLSWRLDADIYETD
Sbjct: 1   MAVPDKDPREEVIQAWYMDDSDEDQRLPHHLEPKQYLSLQQLDELGVLSWRLDADIYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIR DRNYSYMDFCEVCPEKLPNY EKIK+FYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRRDRNYSYMDFCEVCPEKLPNYEEKIKSFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match: A0A5D3D309 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00770 PE=3 SV=1)

HSP 1 Score: 227 bits (579), Expect = 7.57e-74
Identity = 105/108 (97.22%), Postives = 107/108 (99.07%), Query Frame = 0 

Query: 7   DPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKKI 66
           DPREEVIQAW+MDDSDEDQRLPHHLEPKQYVSLQ+LDELGVLSWRLDADIYETDEELKKI
Sbjct: 10  DPREEVIQAWYMDDSDEDQRLPHHLEPKQYVSLQQLDELGVLSWRLDADIYETDEELKKI 69

Query: 67  RHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           RHDRNYSYMDFCEVCPEKLPNY EKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 70  RHDRNYSYMDFCEVCPEKLPNYEEKIKNFYEEHLHTDEEIRYCVAGSG 117

[Close]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match: A0A6J1IG69 (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111473097 PE=3 SV=1)

HSP 1 Score: 226 bits (575), Expect = 2.79e-73
Identity = 105/114 (92.11%), Postives = 110/114 (96.49%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           MAV DKDPREEVIQAW+MDDS+EDQRLPHHL+PKQY+SLQ+LDELGVLSWRLDAD YETD
Sbjct: 1   MAVPDKDPREEVIQAWYMDDSNEDQRLPHHLDPKQYLSLQQLDELGVLSWRLDADKYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 114
           EELKKIR DRNYSYMDFCEVCPEKLPNY EKIKNFYEEHLHTDEEIRYCVAGSG
Sbjct: 61  EELKKIRRDRNYSYMDFCEVCPEKLPNYEEKIKNFYEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. TAIR 10
Match: AT4G14710.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 5.8e-51
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           M  + KD REEVIQAW+MDDS+EDQRLPHH +PK+++SL KL ELGVLSWRLDAD YETD
Sbjct: 1   MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           E+LKKIR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. TAIR 10
Match: AT4G14710.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 5.8e-51
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           M  + KD REEVIQAW+MDDS+EDQRLPHH +PK+++SL KL ELGVLSWRLDAD YETD
Sbjct: 1   MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           E+LKKIR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. TAIR 10
Match: AT4G14710.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 5.8e-51
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           M  + KD REEVIQAW+MDDS+EDQRLPHH +PK+++SL KL ELGVLSWRLDAD YETD
Sbjct: 1   MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           E+LKKIR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. TAIR 10
Match: AT4G14710.4 (RmlC-like cupins superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 5.8e-51
Identity = 90/114 (78.95%), Postives = 101/114 (88.60%), Query Frame = 0 

Query: 1   MAVLDKDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETD 60
           M  + KD REEVIQAW+MDDS+EDQRLPHH +PK+++SL KL ELGVLSWRLDAD YETD
Sbjct: 1   MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60

Query: 61  EELKKIRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           E+LKKIR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAGSG
Sbjct: 61  EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSG 114

[Close]
BLAST of Cucsat.G497 vs. TAIR 10
Match: AT4G14716.1 (acireductone dioxygenase 1 )

HSP 1 Score: 197.2 bits (500), Expect = 7.6e-51
Identity = 89/109 (81.65%), Postives = 99/109 (90.83%), Query Frame = 0 

Query: 6   KDPREEVIQAWFMDDSDEDQRLPHHLEPKQYVSLQKLDELGVLSWRLDADIYETDEELKK 65
           KD REEVIQAW+MDDS+EDQRLPHH +PK++VSL KL ELGVLSWRLDAD YETDE+LKK
Sbjct: 6   KDGREEVIQAWYMDDSEEDQRLPHHKDPKEFVSLDKLAELGVLSWRLDADNYETDEDLKK 65

Query: 66  IRHDRNYSYMDFCEVCPEKLPNYNEKIKNFYEEHLHTDEEIRYCVAGSG 115
           IR  R YSYMDFCEVCPEKLPNY  K+K+F+EEHLHTDEEIRYCVAG+G
Sbjct: 66  IRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGTG 114

The following BLAST results are available for this feature:
BLAST of Cucsat.G497 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F6HDT74.4e-5184.401,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Vitis vinifera OX=2976... [more]
Q8W1088.2e-5078.951,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 OS=Arabidopsis thaliana O... [more]
Q8GXE21.1e-4981.651,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Arabidopsis thaliana O... [more]
O487077.2e-4675.231,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Arabidopsis thaliana O... [more]
A2XCT84.7e-4572.481,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. in... [more]
BLAST of Cucsat.G497 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004134118.15.71e-80100.001,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 [Cucumis sativus] >KGN569... [more]
XP_008438621.15.45e-7897.37PREDICTED: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Cucumis ... [more]
AIS71929.11.73e-7493.861,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 [Citrullus lanatus][more]
XP_038885145.14.95e-7492.981,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Benincasa hispida][more]
KAA0049332.11.56e-7397.221,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2-like [Cucumis melo var. m... [more]
BLAST of Cucsat.G497 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L7D72.76e-80100.001,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis sativus OX=3659 ... [more]
A0A1S3AXH82.64e-7897.371,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis melo OX=3656 GN=... [more]
A0A097BTZ98.37e-7593.861,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Citrullus lanatus OX=365... [more]
A0A5D3D3097.57e-7497.221,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucumis melo var. makuwa... [more]
A0A6J1IG692.79e-7392.111,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Cucurbita maxima OX=3661... [more]
BLAST of Cucsat.G497 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G14710.15.8e-5178.95RmlC-like cupins superfamily protein [more]
AT4G14710.25.8e-5178.95RmlC-like cupins superfamily protein [more]
AT4G14710.35.8e-5178.95RmlC-like cupins superfamily protein [more]
AT4G14710.45.8e-5178.95RmlC-like cupins superfamily protein [more]
AT4G14716.17.6e-5181.65acireductone dioxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 2..86
e-value: 1.2E-25
score: 91.8
IPR004313Acireductone dioxygenase ARD familyPFAMPF03079ARDcoord: 14..77
e-value: 1.2E-10
score: 41.8
IPR004313Acireductone dioxygenase ARD familyPANTHERPTHR23418ACIREDUCTONE DIOXYGENASEcoord: 4..86
NoneNo IPR availablePANTHERPTHR23418:SF141,2-DIHYDROXY-3-KETO-5-METHYLTHIOPENTENE DIOXYGENASEcoord: 4..86
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 14..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G497.T1Cucsat.G497.T1mRNA
Cucsat.G497.T2Cucsat.G497.T2mRNA
Cucsat.G497.T3Cucsat.G497.T3mRNA
Cucsat.G497.T5Cucsat.G497.T5mRNA
Cucsat.G497.T7Cucsat.G497.T7mRNA
Cucsat.G497.T9Cucsat.G497.T9mRNA
Cucsat.G497.T11Cucsat.G497.T11mRNA
Cucsat.G497.T10Cucsat.G497.T10mRNA
Cucsat.G497.T6Cucsat.G497.T6mRNA
Cucsat.G497.T8Cucsat.G497.T8mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019509 L-methionine salvage from methylthioadenosine
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0010309 acireductone dioxygenase [iron(II)-requiring] activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity