Cucsat.G4726 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4726
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	ctg1227: 2483390 .. 2485352 (+)
RNA-Seq Expression	Cucsat.G4726
Synteny	Cucsat.G4726

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTCCCTTTCTTCAACCTCATATCAATTTTCCAAATAAACAGACACCACTTTGTCCAAAATTGCCACCATTTTTAACCTAATCTTCATCTCCTTTCTACCACTCTAAATCTGTTTAAATACCCCTCCACATGCTCTCTAATCTTTGCTCCTTCCCCCAAAACCAAATCTCATACATCATCATTATCATTATCATTATCATGACCACTCTCTCTCAAACTTATCTAGATCATCCTCTCATTCACCATCATCACATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCCGATTCTCACGATTGGTTCAATTCCTCTCTCGAAACCCCTTCTTCTTCTTCTTCTTCTCATCACCATACTAATGTAGATGTTTCAATCCCTTTGATTGATCTAACTGACCCTAATGCAATTTCGCTAATCGGCAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAATCACGACGTGCCAGTAAGTCTAATTGAGAGAGCTGAAGGCGAAACGAGAAGACTGTTCGATCTTCCTATGACACGAAAGCTGAAAGCACTTCGAGCTCCAGGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAAGGGTTTACTATTATGGGGCCTTCCATTGGTCATGCCTCACAACTTTGGCCTTCTAACTACCAACCGTTTTGGTAACTCCTCCATGCACTATTATACACGTAATTAACTAATTTTTTATTTTATTAGTTGTGAAGTACTTAGATTTATACATTCTAGTTAATCATGGTGAGATCTATTTTTAATATATTATAAATAAACTAATTAAACAAAACCTAGCAGTGATCACATAAGAAAATTGTTACAAAAGAAAAAAAATTCAATTTATTAAGAATGCTCTAAGTTTGTATTTTATGGAATATTTAAACCATTATTATAGATTATATATGCTCTTTTAAGTAATTATTTGTGGAATAATTTTGGATTATATTACTATTATATATTTTTTTATTAAAACTATTTAAAAGATATAAAAATAGTAATAATTGAAGATTCTAATCTTTCAATTTTAAATGATAGTAATAATTGAAGAACGTAAAATTAAGCATAGATACCTAATTAAATATGGGTTTGAGATTTTAGGAATATATAGGTGATAATATAACCTTTAGTTTATAATTCTTTTTCAACTATGCTTCTAAATTTGTACTTTGGTCCTTCAAATGAATATATTAAGGCATAATAACATTGGAAAAAAGATTGTTTTTATTAATATATTAATTATTAAACATATAATCGTATAAGAAATCACATATTTTAATTTTATAATGAAGTAGTTAATAATGAATAATTTTCAAATGTAGTGATGTAATGGAAGAGTATCAAAGGAAAATGAAGTCTTTAGCGGAGCAAATCACACATTCAATCTTCAATTACCTTAAAATTTCTGATGGTGCAAACTGGCTCCATTCAGCTGGATCAACTGAAGCCGCCGCTTGCAGCACCGCTCTTCAACTTAACTGCTACCCTCGCTGCCCGGACCCGACTCGGGTCATGGGCTTGGCCCCACACACAGACACATTTCTATTAACCATCCTCCACCAGACCCGCACCTGCGGGCTCCAAGTTTTCAGGGATGGGTTCGGTTGGGTACCGGTGGCGCCGGTACCAGGTGCACTGGTCCTCAACGTCGGGGACTTGTTTCACATCCTTTCGAATGGGCGGTTCCCAAATGTTCTACACCGAGTCGTTGTTGACCCGACCCGACGTCGATTGTCTATGGCTTATTTCTACGGACCGCCACCGGATTTCTGTGTATCGCCGTTGTATGATCCACCAGAATCTCCATGTTACCGGTCTGTGATGGTGAAAGATTATGTTCGGTTGAAGGCAAAGAATCTCGAAAATGCCCTTTCAATGATCCGATTATGAACTACATTATTATGATATATATTGAATT

Coding sequence (CDS)

ATGCTCTCTAATCTTTGCTCCTTCCCCCAAAACCAAATCTCATACATCATCATTATCATTATCATTATCATGACCACTCTCTCTCAAACTTATCTAGATCATCCTCTCATTCACCATCATCACATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCCGATTCTCACGATTGGTTCAATTCCTCTCTCGAAACCCCTTCTTCTTCTTCTTCTTCTCATCACCATACTAATGTAGATGTTTCAATCCCTTTGATTGATCTAACTGACCCTAATGCAATTTCGCTAATCGGCAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAATCACGACGTGCCAGTAAGTCTAATTGAGAGAGCTGAAGGCGAAACGAGAAGACTGTTCGATCTTCCTATGACACGAAAGCTGAAAGCACTTCGAGCTCCAGGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAAGGGTTTACTATTATGGGGCCTTCCATTGGTCATGCCTCACAACTTTGGCCTTCTAACTACCAACCGTTTTGTGATGTAATGGAAGAGTATCAAAGGAAAATGAAGTCTTTAGCGGAGCAAATCACACATTCAATCTTCAATTACCTTAAAATTTCTGATGGTGCAAACTGGCTCCATTCAGCTGGATCAACTGAAGCCGCCGCTTGCAGCACCGCTCTTCAACTTAACTGCTACCCTCGCTGCCCGGACCCGACTCGGGTCATGGGCTTGGCCCCACACACAGACACATTTCTATTAACCATCCTCCACCAGACCCGCACCTGCGGGCTCCAAGTTTTCAGGGATGGGTTCGGTTGGGTACCGGTGGCGCCGGTACCAGGTGCACTGGTCCTCAACGTCGGGGACTTGTTTCACATCCTTTCGAATGGGCGGTTCCCAAATGTTCTACACCGAGTCGTTGTTGACCCGACCCGACGTCGATTGTCTATGGCTTATTTCTACGGACCGCCACCGGATTTCTGTGTATCGCCGTTGTATGATCCACCAGAATCTCCATGTTACCGGTCTGTGATGGTGAAAGATTATGTTCGGTTGAAGGCAAAGAATCTCGAAAATGCCCTTTCAATGATCCGATTATGA

Protein sequence

MLSNLCSFPQNQISYIIIIIIIIMTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL

Homology

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BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 4.3e-89
Identity = 170/359 (47.35%), Postives = 233/359 (64.90%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           M +LS+ Y  HP +H +H  P DF+SL+ +P+S++W  + L+  +   S++       ++
Sbjct: 1   MPSLSEAYRAHP-VHVNHKHP-DFNSLQELPESYNW--THLDDHTLIDSNNIMKESTTTV 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           P+IDL DPNA  LIG AC+TWGV+Q++NH +P+SL+E  +   + LF LP  +K KA R+
Sbjct: 61  PVIDLNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRS 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           P  V+GYG+ RI+ FF K MW+EGFTI+G  + H  +LWP +Y  FCD++ +Y   MK L
Sbjct: 121 PDGVSGYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKL 180

Query: 204 AEQITHSIFNYLKIS-DGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDT 263
           A  +   + + L I+ +   W  S    E A    ALQLN YP CPDP   MGLAPHTD+
Sbjct: 181 AGTLMCLMLDSLGITKEDIKWAGSKAQFEKAC--AALQLNSYPSCPDPDHAMGLAPHTDS 240

Query: 264 FLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 323
             LTIL Q    GLQV R+G GW+ V P+ G LV+NVGDLFHILSNG +P+VLHRV+V+ 
Sbjct: 241 TFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNR 300

Query: 324 TRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 379
           TR+R S+AY YGPP +  + P   L  P + P YRSV   +Y+  KAK+   ALS +RL
Sbjct: 301 TRQRFSVAYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 323.6 bits (828), Expect = 3.1e-87
Identity = 173/358 (48.32%), Postives = 226/358 (63.13%), Query Frame = 0


Query: 27  LSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSS---SSSHHHTNVDVSI 86
           L+  +  HP+   H  +P DF SLR +PDS+ W      TP      S++        +I
Sbjct: 5   LTDVFRGHPIHLPHSHIP-DFTSLRELPDSYKW------TPKDDLLFSAAPSPPATGENI 64

Query: 87  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 146
           PLIDL  P+A + IG+AC TWG FQ+ NH VP+ L++  E  T  LF LP+ RKLK+ R+
Sbjct: 65  PLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARS 124

Query: 147 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 206
              V+GYG+ RI  FF+K MW EGFTI G  +    +LWP ++  +CD++EEY+  MK L
Sbjct: 125 ETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKL 184

Query: 207 AEQITHSIFNYLKIS-DGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDT 266
           A ++     N L +S +   W  ++ S++      ALQLN YP CP+P R MGLA HTD+
Sbjct: 185 ASKLMWLALNSLGVSEEDIEW--ASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDS 244

Query: 267 FLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 326
            LLTIL+Q  T GLQVFRD  GWV V P PG+LV+NVGDLFHILSNG F +VLHR  V+ 
Sbjct: 245 TLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQ 304

Query: 327 TRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 378
           TR RLS+A+ +GP  D  +SP   L  P ESP Y+SV  K+Y+R KA +   ALSMIR
Sbjct: 305 TRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

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BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 308.5 bits (789), Expect = 1.0e-82
Identity = 163/357 (45.66%), Postives = 222/357 (62.18%), Query Frame = 0


Query: 25  TTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSIP 84
           +TLS  +  HP IH     P DF SL   PDS+ W        S+S+S       D ++P
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASAS-------DETLP 62

Query: 85  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 144
           LIDL+D +  +L+G+AC TWG FQ+ NH VP  L++  E  T  LF LP+ RKLKA R+ 
Sbjct: 63  LIDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSE 122

Query: 145 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 204
             V+GYG+ RI  FF+K MW EGFT++G  +    +LWPS++  +C+++EEY+  M+ LA
Sbjct: 123 NGVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLA 182

Query: 205 EQITHSIFNYLKISD-GANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 264
            ++       L + +    W  +  +++       +QLN YP+CP+P R MGLA HTD+ 
Sbjct: 183 AKLMWFALGSLGVEEKDIQW--AGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDST 242

Query: 265 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 324
           L+TIL+Q  T GLQVFRD  GWV   PVPG+LV+NVGDL HIL+NG FP+VLHR  V+  
Sbjct: 243 LMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV 302

Query: 325 RRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 378
           R R SMAY +GPP D  +SP   L DP +SP Y S+  K Y+  KA +   +LS+IR
Sbjct: 303 RSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 4.4e-78
Identity = 146/336 (43.45%), Postives = 217/336 (64.58%), Query Frame = 0


Query: 49  SLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQ 108
           SL+T+P+S  W  ++ ++    SS+     V+ SIP+IDL++P+  +LIG+A +TWG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 109 LINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGF 168
           + NH +   L++  E  ++ LFD+P  RKL+A  +   V+GYG PRI+PFF K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 169 TIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKIS-DGANWLHS 228
           TI   S   H + LWP ++  +C +++EY  +M+ LA ++ + I   L ++ +   W H 
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 229 AGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWV 288
              + +     A++LN YP CP+P R MGLA HTD+ +LTILHQ+ T GLQVFR+  GWV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 289 PVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFY-GPPPDFCVSP-- 348
            V P PG LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  ++P  
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 349 -LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 379
            L  P E   YRS+  K+Y+++K +  + A+  IR+
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.8e-75
Identity = 146/361 (40.44%), Postives = 220/361 (60.94%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           M++++Q + ++P ++   I+PLDF + +T+PDSH W     ET S              I
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPETTSG------------PI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           P+I L++P    L+  ACE WGVF + +H V  SL+   + + +RLF LPM RK+ A+R+
Sbjct: 61  PVISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRS 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKS 203
           P + TGYG+ RI+ F+ K MW EGF++MG S+  HA+ LWP ++  FC+VMEEYQ+ M  
Sbjct: 121 PDESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDD 180

Query: 204 LAEQITHSIFNYLKIS-DGANWL-HSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHT 263
           L+ ++   +   L ++ +   WL      +   +  + LQLN YP CPDP   MGLAPHT
Sbjct: 181 LSHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHT 240

Query: 264 DTFLLTILHQTRTCGLQV---FRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHR 323
           D+ LLTIL+Q    GL++     +G  W+ V P+ G+LV+ +GDL HI+SNG+F + +HR
Sbjct: 241 DSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHR 300

Query: 324 VVVDPTRRRLSMAYFYGPPPDFCVSPL-YDPPESPCYRSVMVKDYVRLKAKNLENALSMI 378
            VV+ T  R+S AYF GPP +  + PL  D    P YR ++ ++Y+  KA +   AL++ 
Sbjct: 301 AVVNKTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLF 348

[Close]

BLAST of Cucsat.G4726 vs. NCBI nr
Match: KGN62899.1 (hypothetical protein Csa_021969 [Cucumis sativus])

HSP 1 Score: 758 bits (1958), Expect = 1.68e-276
Identity = 369/378 (97.62%), Postives = 371/378 (98.15%), Query Frame = 0


Query: 1   MLSNLCSFPQNQISYIIIIIIIIMTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60
           MLSNLCSFPQNQISYII+I    MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF
Sbjct: 1   MLSNLCSFPQNQISYIIVI----MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60

Query: 61  NSSLETPSSSSSSHHHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE 120
           NSSLETPSSSS  HHHTNV+VSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE
Sbjct: 61  NSSLETPSSSS--HHHTNVNVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE 120

Query: 121 RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ 180
           RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ
Sbjct: 121 RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ 180

Query: 181 LWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQ 240
           LWPSNYQPFCDVMEEYQRKMKSLAEQIT SIFNYLKISDGANWLHSAGSTEAAACSTALQ
Sbjct: 181 LWPSNYQPFCDVMEEYQRKMKSLAEQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQ 240

Query: 241 LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG 300
           LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG
Sbjct: 241 LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG 300

Query: 301 DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD 360
           DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD
Sbjct: 301 DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD 360

Query: 361 YVRLKAKNLENALSMIRL 378
           YVRLKAKNLENALSMIRL
Sbjct: 361 YVRLKAKNLENALSMIRL 372

[Close]

BLAST of Cucsat.G4726 vs. NCBI nr
Match: NP_001274405.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28483.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 729 bits (1881), Expect = 4.48e-265
Identity = 351/355 (98.87%), Postives = 352/355 (99.15%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS  HHHTNV+VSI
Sbjct: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS--HHHTNVNVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA
Sbjct: 61  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL
Sbjct: 121 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 180

Query: 204 AEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 263
           AEQIT SIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF
Sbjct: 181 AEQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 240

Query: 264 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 323
           LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT
Sbjct: 241 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 300

Query: 324 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 353

[Close]

BLAST of Cucsat.G4726 vs. NCBI nr
Match: XP_008445137.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 682 bits (1760), Expect = 2.26e-246
Identity = 334/379 (88.13%), Postives = 351/379 (92.61%), Query Frame = 0


Query: 1   MLSNLCSFPQNQISYIIIIIIIIMTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60
           MLSNLCSFP+  ++        IMTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW 
Sbjct: 1   MLSNLCSFPKTNLT--------IMTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWL 60

Query: 61  NSSLETPSSSSSSH-HHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLI 120
           +SS ETPSSSSSS  +HTNVDVSIPLIDLTD +AI+LIGNACETWGVFQLINHDVP+SLI
Sbjct: 61  HSSPETPSSSSSSSCYHTNVDVSIPLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLI 120

Query: 121 ERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHAS 180
           E+AEGETRRLFDLPMTRKLKALR P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HAS
Sbjct: 121 EKAEGETRRLFDLPMTRKLKALRTPRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHAS 180

Query: 181 QLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTAL 240
           QLWPSNYQPFCDVMEEYQRKMK+LAEQIT+ IFNYLKISD ANWLHS G+  AAACSTAL
Sbjct: 181 QLWPSNYQPFCDVMEEYQRKMKALAEQITNLIFNYLKISDCANWLHSTGA--AAACSTAL 240

Query: 241 QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNV 300
           QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQ+FRDG GWVPVAP+PGALVLNV
Sbjct: 241 QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNV 300

Query: 301 GDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVK 360
           GDLFHILSNGRFPNVLHRVVVDPT+RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VK
Sbjct: 301 GDLFHILSNGRFPNVLHRVVVDPTQRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVK 360

Query: 361 DYVRLKAKNLENALSMIRL 378
           DYVRLKAKNLENALSMIRL
Sbjct: 361 DYVRLKAKNLENALSMIRL 369

[Close]

BLAST of Cucsat.G4726 vs. NCBI nr
Match: KAA0064970.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 667 bits (1721), Expect = 1.05e-240
Identity = 323/355 (90.99%), Postives = 337/355 (94.93%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS E PSSSSS +H TNVDVSI
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPEIPSSSSSCYH-TNVDVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           PLIDLTD +AI+LIGNACETWGVFQLINHDVP+SLIE+AEGETRRLFDLPMTRKLKALR 
Sbjct: 61  PLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALRT 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+L
Sbjct: 121 PRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKAL 180

Query: 204 AEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 263
           AEQIT+ IFNYLKISDGANWLHS G+  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTF
Sbjct: 181 AEQITNLIFNYLKISDGANWLHSTGA--AAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 240

Query: 264 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 323
           LLTILHQTRTCGLQ+FRDG GWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDPT
Sbjct: 241 LLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 300

Query: 324 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           +RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 301 QRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 352

[Close]

BLAST of Cucsat.G4726 vs. NCBI nr
Match: XP_038886716.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 631 bits (1628), Expect = 1.55e-226
Identity = 309/358 (86.31%), Postives = 322/358 (89.94%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQTY DHPLIHHHHIVPLD DSLRTIPDSHDW  SS +TPSSS    HHTN DVSI
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDLDSLRTIPDSHDWSGSSPDTPSSSG---HHTNEDVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           P IDL DPNA  LIGNACETWGVFQLINHDVP++ I+ AEGETRRLFDLPMTRKLKALRA
Sbjct: 61  PSIDLNDPNATMLIGNACETWGVFQLINHDVPLTFIDTAEGETRRLFDLPMTRKLKALRA 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           P   TGYGLPRITPFFSKYMWHEGFTIMG S+ HASQLWPSNYQPFCDVMEEYQRKMKSL
Sbjct: 121 PDHATGYGLPRITPFFSKYMWHEGFTIMGSSVDHASQLWPSNYQPFCDVMEEYQRKMKSL 180

Query: 204 AEQITHSIFNYLKISDGA---NWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHT 263
           AEQITH IFN+LKIS+ A   NWLHS   TEAA CSTALQLNCYPRCPDPTRVMGLAPHT
Sbjct: 181 AEQITHFIFNFLKISNDAKITNWLHS---TEAATCSTALQLNCYPRCPDPTRVMGLAPHT 240

Query: 264 DTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVV 323
           DTFLLTILHQ+RTCGLQVFRDGFGWV VAPV GALV+NVGDLFHI+SNGRFPNVLHRVVV
Sbjct: 241 DTFLLTILHQSRTCGLQVFRDGFGWVSVAPVAGALVINVGDLFHIMSNGRFPNVLHRVVV 300

Query: 324 DPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           DPTRRRLSMAYFYGPPPDFCVSPL DP +SP YRSV+VKDYVRLK KNLENALSMIR+
Sbjct: 301 DPTRRRLSMAYFYGPPPDFCVSPLNDPLKSPRYRSVVVKDYVRLKGKNLENALSMIRI 352

[Close]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL
Match: A0A0A0LPF2 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379300 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 8.13e-277
Identity = 369/378 (97.62%), Postives = 371/378 (98.15%), Query Frame = 0


Query: 1   MLSNLCSFPQNQISYIIIIIIIIMTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60
           MLSNLCSFPQNQISYII+I    MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF
Sbjct: 1   MLSNLCSFPQNQISYIIVI----MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60

Query: 61  NSSLETPSSSSSSHHHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE 120
           NSSLETPSSSS  HHHTNV+VSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE
Sbjct: 61  NSSLETPSSSS--HHHTNVNVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIE 120

Query: 121 RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ 180
           RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ
Sbjct: 121 RAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQ 180

Query: 181 LWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQ 240
           LWPSNYQPFCDVMEEYQRKMKSLAEQIT SIFNYLKISDGANWLHSAGSTEAAACSTALQ
Sbjct: 181 LWPSNYQPFCDVMEEYQRKMKSLAEQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQ 240

Query: 241 LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG 300
           LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG
Sbjct: 241 LNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVG 300

Query: 301 DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD 360
           DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD
Sbjct: 301 DLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKD 360

Query: 361 YVRLKAKNLENALSMIRL 378
           YVRLKAKNLENALSMIRL
Sbjct: 361 YVRLKAKNLENALSMIRL 372

[Close]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL
Match: S0BE23 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox2 PE=2 SV=1)

HSP 1 Score: 729 bits (1881), Expect = 2.17e-265
Identity = 351/355 (98.87%), Postives = 352/355 (99.15%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS  HHHTNV+VSI
Sbjct: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS--HHHTNVNVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA
Sbjct: 61  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL
Sbjct: 121 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 180

Query: 204 AEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 263
           AEQIT SIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF
Sbjct: 181 AEQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 240

Query: 264 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 323
           LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT
Sbjct: 241 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 300

Query: 324 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 353

[Close]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL
Match: A0A1S3BCU4 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488262 PE=3 SV=1)

HSP 1 Score: 682 bits (1760), Expect = 1.09e-246
Identity = 334/379 (88.13%), Postives = 351/379 (92.61%), Query Frame = 0


Query: 1   MLSNLCSFPQNQISYIIIIIIIIMTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWF 60
           MLSNLCSFP+  ++        IMTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW 
Sbjct: 1   MLSNLCSFPKTNLT--------IMTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWL 60

Query: 61  NSSLETPSSSSSSH-HHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLI 120
           +SS ETPSSSSSS  +HTNVDVSIPLIDLTD +AI+LIGNACETWGVFQLINHDVP+SLI
Sbjct: 61  HSSPETPSSSSSSSCYHTNVDVSIPLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLI 120

Query: 121 ERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHAS 180
           E+AEGETRRLFDLPMTRKLKALR P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HAS
Sbjct: 121 EKAEGETRRLFDLPMTRKLKALRTPRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHAS 180

Query: 181 QLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTAL 240
           QLWPSNYQPFCDVMEEYQRKMK+LAEQIT+ IFNYLKISD ANWLHS G+  AAACSTAL
Sbjct: 181 QLWPSNYQPFCDVMEEYQRKMKALAEQITNLIFNYLKISDCANWLHSTGA--AAACSTAL 240

Query: 241 QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNV 300
           QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQ+FRDG GWVPVAP+PGALVLNV
Sbjct: 241 QLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNV 300

Query: 301 GDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVK 360
           GDLFHILSNGRFPNVLHRVVVDPT+RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VK
Sbjct: 301 GDLFHILSNGRFPNVLHRVVVDPTQRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVK 360

Query: 361 DYVRLKAKNLENALSMIRL 378
           DYVRLKAKNLENALSMIRL
Sbjct: 361 DYVRLKAKNLENALSMIRL 369

[Close]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL
Match: A0A5A7VCQ3 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002910 PE=3 SV=1)

HSP 1 Score: 667 bits (1721), Expect = 5.08e-241
Identity = 323/355 (90.99%), Postives = 337/355 (94.93%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS E PSSSSS +H TNVDVSI
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPEIPSSSSSCYH-TNVDVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           PLIDLTD +AI+LIGNACETWGVFQLINHDVP+SLIE+AEGETRRLFDLPMTRKLKALR 
Sbjct: 61  PLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALRT 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+L
Sbjct: 121 PRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKAL 180

Query: 204 AEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 263
           AEQIT+ IFNYLKISDGANWLHS G+  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTF
Sbjct: 181 AEQITNLIFNYLKISDGANWLHSTGA--AAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 240

Query: 264 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 323
           LLTILHQTRTCGLQ+FRDG GWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDPT
Sbjct: 241 LLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 300

Query: 324 RRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           +RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 301 QRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 352

[Close]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL
Match: I0J1I8 (Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox4 PE=2 SV=1)

HSP 1 Score: 590 bits (1522), Expect = 9.72e-211
Identity = 288/358 (80.45%), Postives = 309/358 (86.31%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           MTTLSQ Y DHPLIH HHIVPLDFDSLRT+PDSHDW +SS +T         HTN DVSI
Sbjct: 1   MTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDTLKL------HTNGDVSI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           PLID++DPN   LIGN C+TWGVFQL NH VP SL+ERAE E  RLFDLPMTRKLKALRA
Sbjct: 61  PLIDMSDPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRA 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 203
           PGD TGYGLPRITPFFSKYMWHEGFTIMG S  HASQLW SNYQPFCDVMEEYQ+ MKSL
Sbjct: 121 PGDATGYGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSL 180

Query: 204 AEQITHSIFNYLKISDGA---NWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHT 263
           AE+ITH I N+LKI D A   NWLHSAG+  AAACSTALQLNCYPRCPDPTRVMGLAPHT
Sbjct: 181 AEKITHLILNFLKIPDDAEISNWLHSAGA--AAACSTALQLNCYPRCPDPTRVMGLAPHT 240

Query: 264 DTFLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVV 323
           DTFLLTILHQ+ TCGLQ+FRDGFGW+PV PV GALVLNVGDLFHILSNGRFPNVLHRVVV
Sbjct: 241 DTFLLTILHQSSTCGLQIFRDGFGWIPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVV 300

Query: 324 DPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 378
           DPTRRRLSMAYF+GPPPDFCV+P  +PPESP +RSV+VK+YV LKAKNLE ALSMI +
Sbjct: 301 DPTRRRLSMAYFFGPPPDFCVTPFNNPPESPRFRSVVVKEYVGLKAKNLECALSMIEI 350

[Close]

BLAST of Cucsat.G4726 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 323.6 bits (828), Expect = 2.2e-88
Identity = 173/358 (48.32%), Postives = 226/358 (63.13%), Query Frame = 0


Query: 27  LSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSS---SSSHHHTNVDVSI 86
           L+  +  HP+   H  +P DF SLR +PDS+ W      TP      S++        +I
Sbjct: 5   LTDVFRGHPIHLPHSHIP-DFTSLRELPDSYKW------TPKDDLLFSAAPSPPATGENI 64

Query: 87  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 146
           PLIDL  P+A + IG+AC TWG FQ+ NH VP+ L++  E  T  LF LP+ RKLK+ R+
Sbjct: 65  PLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARS 124

Query: 147 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSL 206
              V+GYG+ RI  FF+K MW EGFTI G  +    +LWP ++  +CD++EEY+  MK L
Sbjct: 125 ETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKL 184

Query: 207 AEQITHSIFNYLKIS-DGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDT 266
           A ++     N L +S +   W  ++ S++      ALQLN YP CP+P R MGLA HTD+
Sbjct: 185 ASKLMWLALNSLGVSEEDIEW--ASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDS 244

Query: 267 FLLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 326
            LLTIL+Q  T GLQVFRD  GWV V P PG+LV+NVGDLFHILSNG F +VLHR  V+ 
Sbjct: 245 TLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQ 304

Query: 327 TRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 378
           TR RLS+A+ +GP  D  +SP   L  P ESP Y+SV  K+Y+R KA +   ALSMIR
Sbjct: 305 TRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Cucsat.G4726 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 308.5 bits (789), Expect = 7.3e-84
Identity = 163/357 (45.66%), Postives = 222/357 (62.18%), Query Frame = 0


Query: 25  TTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSIP 84
           +TLS  +  HP IH     P DF SL   PDS+ W        S+S+S       D ++P
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTWTPKDDLLFSASAS-------DETLP 62

Query: 85  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 144
           LIDL+D +  +L+G+AC TWG FQ+ NH VP  L++  E  T  LF LP+ RKLKA R+ 
Sbjct: 63  LIDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSE 122

Query: 145 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 204
             V+GYG+ RI  FF+K MW EGFT++G  +    +LWPS++  +C+++EEY+  M+ LA
Sbjct: 123 NGVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLA 182

Query: 205 EQITHSIFNYLKISD-GANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTF 264
            ++       L + +    W  +  +++       +QLN YP+CP+P R MGLA HTD+ 
Sbjct: 183 AKLMWFALGSLGVEEKDIQW--AGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDST 242

Query: 265 LLTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPT 324
           L+TIL+Q  T GLQVFRD  GWV   PVPG+LV+NVGDL HIL+NG FP+VLHR  V+  
Sbjct: 243 LMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV 302

Query: 325 RRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 378
           R R SMAY +GPP D  +SP   L DP +SP Y S+  K Y+  KA +   +LS+IR
Sbjct: 303 RSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Cucsat.G4726 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 293.1 bits (749), Expect = 3.2e-79
Identity = 146/336 (43.45%), Postives = 217/336 (64.58%), Query Frame = 0


Query: 49  SLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSIPLIDLTDPNAISLIGNACETWGVFQ 108
           SL+T+P+S  W  ++ ++    SS+     V+ SIP+IDL++P+  +LIG+A +TWG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 109 LINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGF 168
           + NH +   L++  E  ++ LFD+P  RKL+A  +   V+GYG PRI+PFF K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 169 TIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKIS-DGANWLHS 228
           TI   S   H + LWP ++  +C +++EY  +M+ LA ++ + I   L ++ +   W H 
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 229 AGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGFGWV 288
              + +     A++LN YP CP+P R MGLA HTD+ +LTILHQ+ T GLQVFR+  GWV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 289 PVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFY-GPPPDFCVSP-- 348
            V P PG LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  ++P  
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 349 -LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 379
            L  P E   YRS+  K+Y+++K +  + A+  IR+
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Cucsat.G4726 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 282.3 bits (721), Expect = 5.6e-76
Identity = 146/361 (40.44%), Postives = 220/361 (60.94%), Query Frame = 0


Query: 24  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSSSHHHTNVDVSI 83
           M++++Q + ++P ++   I+PLDF + +T+PDSH W     ET S              I
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPETTSG------------PI 60

Query: 84  PLIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRA 143
           P+I L++P    L+  ACE WGVF + +H V  SL+   + + +RLF LPM RK+ A+R+
Sbjct: 61  PVISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRS 120

Query: 144 PGDVTGYGLPRITPFFSKYMWHEGFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKS 203
           P + TGYG+ RI+ F+ K MW EGF++MG S+  HA+ LWP ++  FC+VMEEYQ+ M  
Sbjct: 121 PDESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDD 180

Query: 204 LAEQITHSIFNYLKIS-DGANWL-HSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHT 263
           L+ ++   +   L ++ +   WL      +   +  + LQLN YP CPDP   MGLAPHT
Sbjct: 181 LSHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHT 240

Query: 264 DTFLLTILHQTRTCGLQV---FRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHR 323
           D+ LLTIL+Q    GL++     +G  W+ V P+ G+LV+ +GDL HI+SNG+F + +HR
Sbjct: 241 DSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHR 300

Query: 324 VVVDPTRRRLSMAYFYGPPPDFCVSPL-YDPPESPCYRSVMVKDYVRLKAKNLENALSMI 378
            VV+ T  R+S AYF GPP +  + PL  D    P YR ++ ++Y+  KA +   AL++ 
Sbjct: 301 AVVNKTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLF 348

[Close]

BLAST of Cucsat.G4726 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.3e-44
Identity = 102/299 (34.11%), Postives = 158/299 (52.84%), Query Frame = 0


Query: 77  TNVDVSIPLIDLTD----PNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDL 136
           ++  + IP++D+ D    P  + L+ +ACE WG FQ++NH V  SL+ER  G  R  F+L
Sbjct: 42  SDAGIEIPVLDMNDVWGKPEGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFEL 101

Query: 137 PMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTI--MGPSIGHASQLWPSNYQPFC 196
           P+  K K   +P    GYG        +K  W + F +  +  SI + S+ WPS      
Sbjct: 102 PLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSK-WPSQPPKIR 161

Query: 197 DVMEEYQRKMKSLAEQITHSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDP 256
           +++E+Y  +++ L E++T ++   L +          G  +  A   +L+ N YP+CP P
Sbjct: 162 ELIEKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGA---SLRTNFYPKCPQP 221

Query: 257 TRVMGLAPHTDTFLLTI-LHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNG 316
              +GL+ H+D   +TI L   +  GLQV R G GWV +  VP AL++N+GD   ILSNG
Sbjct: 222 QLTLGLSSHSDPGGITILLPDEKVAGLQV-RRGDGWVTIKSVPNALIVNIGDQLQILSNG 281

Query: 317 RFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSP-----------LYDPPESPCYRSVM 358
            + +V H+V+V+    R+S+A+FY P  D  V P           LY P     YRS++
Sbjct: 282 IYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLI 335

The following BLAST results are available for this feature:

BLAST of Cucsat.G4726 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	4.3e-89	47.35	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q39103	3.1e-87	48.32	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	1.0e-82	45.66	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q9C971	4.4e-78	43.45	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	7.8e-75	40.44	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Cucsat.G4726 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62899.1	1.68e-276	97.62	hypothetical protein Csa_021969 [Cucumis sativus]	[more]
NP_001274405.1	4.48e-265	98.87	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28483.1 GA 3-oxidase...	[more]
XP_008445137.1	2.26e-246	88.13	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
KAA0064970.1	1.05e-240	90.99	gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa]	[more]
XP_038886716.1	1.55e-226	86.31	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]

BLAST of Cucsat.G4726 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPF2	8.13e-277	97.62	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G...	[more]
S0BE23	2.17e-265	98.87	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox2 PE=2 SV=1	[more]
A0A1S3BCU4	1.09e-246	88.13	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488262 PE...	[more]
A0A5A7VCQ3	5.08e-241	90.99	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
I0J1I8	9.72e-211	80.45	Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox4 PE=2 SV=1	[more]

BLAST of Cucsat.G4726 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	2.2e-88	48.32	gibberellin 3-oxidase 1	[more]
AT1G80340.1	7.3e-84	45.66	gibberellin 3-oxidase 2	[more]
AT1G80330.1	3.2e-79	43.45	gibberellin 3-oxidase 4	[more]
AT4G21690.1	5.6e-76	40.44	gibberellin 3-oxidase 3	[more]
AT2G38240.1	4.3e-44	34.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 238..335 e-value: 2.2E-25 score: 89.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 83..173 e-value: 1.0E-16 score: 61.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 44..372 e-value: 3.7E-77 score: 261.8
None	No IPR available	PANTHER	PTHR47990:SF105	GIBBERELLIN 2-BETA-DIOXYGENASE	coord: 35..372
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 35..372
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 40..368
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 235..335 score: 13.073103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4726.T1	Cucsat.G4726.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding