Cucsat.G4520 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4520
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description1-aminocyclopropane-1-carboxylate oxidase
Locationctg1216: 1012206 .. 1016190 (+)
RNA-Seq ExpressionCucsat.G4520
SyntenyCucsat.G4520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAGAAAGAAATAAATAAAGAAGAAGACGATGAATGAATCACACAGTTAGTTAGAATTATATAAAGAAAGACAGAAGCTTCAAAAAAGAAAGAAACAGAGTAGACCCATTAAAAAGCTCTCCAATCCAGTATGATTTCCTCCATGTTCCTCAATTTCATGACATCCCATCATTACTTCTTCACCTTTCCCTTTTTCCCTTTTAATTTCTTCATTTCCTTCAACCTTATTGGAAAACCAAAACCCTTCTTCCTCCACTTCCCCTGTTTTCTTTTCAATATCCATGGTAATGATGTCGCCTGAACGCGCCGCGGCGACTCCCTTCGAATTCCGGGCACCGCCGCCATCTCCCATCAGAACCAGTCGGCGGTCCTCCGTCACAAACGATGAGGTTCTCACAGAATTCCTCGAGCACTCTCTCCGTGTCCCTGATCTCGTTTTGCCGGATACCATTTTCCCGAGGGAGAGATTTGTTGAGCACCCACCGAGAATCGACTATAGGTTGATTGAATCGTACGATCGTGATTCTGTTTTGAAGATTTTGGATTCAATGTGTAGCTTTGGGTTTTTCCAATTGGTGAACCATGGGATTCCGGTAGAGCTAATTGGGGCAGTTGCGGGGGCGGCGGATGCAGGGGTTTTTGGGATATCGCCGGAGAAGAAGGTGGGGGTGGGGAGGTCGCCGGAGAAGGCGTATGGGTTTGAGGAGTATTGGCATGGAGAGGATGAGAGTGAGGTTAGTGAAGAGTTTGTGTGGAGCAGAGATGAAGATTTGAAAATGGAAATGGAGGTCATTTCGCCTATTGGATATTCCAATTTCAGGTTATTTTTCTTTTCTTATTATCCAAATATTATCAATTTTATATTAAATATATGAGTTTCCTTATTATCATTGTTATTATTTATTTGAGAGGGATTAAACATTTAAATTTAATACATTGTGTTTTCAAATTAAAAAAATTAATATATGATCAATAATTGACATCTACTCTGTATGTATATGTATGTATATATAGATGAGTTTTTTTTATCTCTATTAAGAGTATTCAAAATGAAAGTCAGAAAAAGAAAGACCCTTCTTTATAAATCATTTTGTATTTTATTTTTTGTTTTTGAATATTGAATTTGTTTACTCACGTTTTCTTATTCATCAGTTTCATATTTCGTTTACAAATATTTCTAAGTCTTAACCAACTTTTAAAAATAAAAAAATCACTTTAAAATTTATTTTTCTAAAAAGCAAATGTGACTTTTAGATTGAAAACACCACTAAAATTTATATTTATATGAAAGAAATATTAAATAATAGATAGAAATGATATTTTCATTCTAAATCTAAAAAACCAAAAAAGAAAATAAACCAAAGGGTTATAAAAAACTCATTTTTGCTTTTAGAAATCAACAAAAATATCTAATACATACCTACGATGACGACGATAAGTAAAAAATGGGTGAGAAAAGTACAATTTGGCCTTAAAACAATATTCCCAAAAGATTTAAACATCTTGTAACCATTCCATCTAGGTAATCTTTTTAAAAAAGTTGTAACAAGAATTCAACTTTCCAATATTCCCAAAATGAAAGATTGTGTTTCTTTTAATTTCAACAAACGAATGACAAGCAATATTACAACTCAAATTAAAATTTTGTTTTAATGTAACAAAAAAAAAGTCCATTTCCAAATACACTAATTGGGAAATTGCATACAAATCTAAAAACTCATTATATATTTCGTGCATCTCTTTGTTCATGCAGCAAGAAAATGGAATGGCTAACACAACTAACAGAGAAATTGGGAGAGAAAATGTTAGGAATTTTTCTAGAAAACAGTGGGAAAATTGCAAGAAATGAAGTGGTTTTAGGGCATGGATCAGTGTGGTGTGTTTACAAACACAAGATCAAGCAAGGATTGAGTGTTTGGAACGATGAATTAGAAAACTGTTTCAAACATGATGTCATCAGAATGCTCATAAGAGGAACTGATTTCTCTCATGCTTTTTGCTTCCATTTCTGCCATGGATCCTCTCCTCTATTCCATGCTTACTCCAAGAGAGGTTGGGTTTCTTTTGTACCCGATGATTCCGCCATTGTTGTCACCGTCGGTGATCATATTCAGGTACGTCCTACTTCTACTCTAATTTGATGATGATTTTGTAGTAGAATCACCATGGAAAGAAAAAAAATATATTAAGGAATACATAAGAGTAAAGATGATAAACGTGAGTACTGTGTTTCTCCCTATGTCCACATTTAATTCTCTAATCCACAAAAATTTGTTAAGTTTTGTGAGGTTGATGATTGAATTGTTTTCATGTGATAAATCATTTTTTGTTTTGTTTTCTTAAATTATAATTCAATTAAATAAATTCTATTTTGAGTTTTTTTTTAAAAAAATTAACTAGTTAATTTTCACTAAAGAATTTCATTTAAAAAGTTAAATTGAATAAGAAAATCTCCTATGTTAATAGACAGTTATTTACTTCCTAGTTTAATTGAAACAAAATAATTAACAATAACCTATCAATTCATATTATGGACATATTTGTAATCCAAATACTGCTCTATCTATATAAAATTAAATACATCAAATTATCAATGTAATTAACAAAAAAAAAAAAAAAATGAAATTGTCGACCAAAGCTTAGGTCATATGTGATTCCTAAATAACAAGAACAACACTAACATATGGCAAGGGGAAAATAGAGTTGATATGTACTAGCTTGTATCTATTATTTTTAGTTTAAGAAGTATGGCGAAGAGATTCAAATCTATGATCTTTTGGTCAATAATTAATACTTTATATGTCCAGTTGAGCTTTGTTCATTTTGACGAGTGTTTCATCTTTGTTTAACTTATGAAAAAAATATTCCATTTTTCACCACGTTCATATTGAGAGAGAATGGAAGATGTCCATTACTTTTCTATTAACACAACTATAGTTTATAAATGAGGAATGAAATATACATATATATAAAGTTGTGAAAGAATTTTGCATAACAAATTATAGAGTCAAAGATTAAGTCTCACCCATTTTTAAGACTAAAGGTTATATTGGTTATCATTCAGCTATATTCACTCTAAGTTAAAGTTAGAGTTTCAAGGACTTCATTGTATTTGTAGCAAAGAAAGTTAACTACTTTTCCTAATTACGATGATGCCATAAGTCTACTTTTACAAAGGTCATGGTTGAGTCATAAATTTTTCTAAAGACAGAGAGAGTGTAACGGTGTGTTTTAATTATTTGAACAATACTAAAATTATTGAGATTAGATGGGAAAAAACTGAAAATCAATTCAATTCAAATTAATGAAGATGACAAAATTAAAAACTTCCATAAATGTAAACTCTTTTTCTTTATATAGAATTTTACAAAAATGGGCATCATTTGTAATTGAACGTGGATCCACTCCAAAAGTTGGTGTAATTATAACATTAATTAAAGTTGAATTAAGAAAAGGGGTTGAAATATTTAAATTAAGTGAAACAAAATTTAATTGTATCCCTTTCTTTGCATCAACTTTTGTTTTTCTCTCTCTCTAAATTATAATAAAATTAAACCTCAGGGTTGGAGTGGTGGGCAATACAAGCATGTGATTGGTAGGCCAATCTATAAAGATCATAACAAAGAGAAACAGAGTGGGAGCAATAATAATACTAATGGCATCTCAATGGCTTTTCTCTTTTCTCCAACTCCTTCTTCATCATCATCAAATGCCCTCAAGTCCCCCAATGAAATTAGGACTCTTTCATTAGCCCATCAAGCTCTATTTGCTCTCTTCTTAACTCTTTTTTACAATTTTTTCTTTTATATTCTCAAATACATTTGACTATGGTTTGCCTTTCTTTCTATTTATGTTTCTTTCATTTATCCCATATGTATCACCTTTCATTTAAAATGAGATATATAAAATATGAGTTTTCTTTCGTTTGAGTTAGTATATGTACACGAGAATGATTCTAGTTGTGTTACTATCATTTTCTTT

Coding sequence (CDS)

ATGGTAATGATGTCGCCTGAACGCGCCGCGGCGACTCCCTTCGAATTCCGGGCACCGCCGCCATCTCCCATCAGAACCAGTCGGCGGTCCTCCGTCACAAACGATGAGGTTCTCACAGAATTCCTCGAGCACTCTCTCCGTGTCCCTGATCTCGTTTTGCCGGATACCATTTTCCCGAGGGAGAGATTTGTTGAGCACCCACCGAGAATCGACTATAGGTTGATTGAATCGTACGATCGTGATTCTGTTTTGAAGATTTTGGATTCAATGTGTAGCTTTGGGTTTTTCCAATTGGTGAACCATGGGATTCCGGTAGAGCTAATTGGGGCAGTTGCGGGGGCGGCGGATGCAGGGGTTTTTGGGATATCGCCGGAGAAGAAGGTGGGGGTGGGGAGGTCGCCGGAGAAGGCGTATGGGTTTGAGGAGTATTGGCATGGAGAGGATGAGAGTGAGGTTAGTGAAGAGTTTGTGTGGAGCAGAGATGAAGATTTGAAAATGGAAATGGAGGTCATTTCGCCTATTGGATATTCCAATTTCAGCAAGAAAATGGAATGGCTAACACAACTAACAGAGAAATTGGGAGAGAAAATGTTAGGAATTTTTCTAGAAAACAGTGGGAAAATTGCAAGAAATGAAGTGGTTTTAGGGCATGGATCAGTGTGGTGTGTTTACAAACACAAGATCAAGCAAGGATTGAGTGTTTGGAACGATGAATTAGAAAACTGTTTCAAACATGATGTCATCAGAATGCTCATAAGAGGAACTGATTTCTCTCATGCTTTTTGCTTCCATTTCTGCCATGGATCCTCTCCTCTATTCCATGCTTACTCCAAGAGAGGTTGGGTTTCTTTTGTACCCGATGATTCCGCCATTGTTGTCACCGTCGGTGATCATATTCAGGGTTGGAGTGGTGGGCAATACAAGCATGTGATTGGTAGGCCAATCTATAAAGATCATAACAAAGAGAAACAGAGTGGGAGCAATAATAATACTAATGGCATCTCAATGGCTTTTCTCTTTTCTCCAACTCCTTCTTCATCATCATCAAATGCCCTCAAGTCCCCCAATGAAATTAGGACTCTTTCATTAGCCCATCAAGCTCTATTTGCTCTCTTCTTAACTCTTTTTTACAATTTTTTCTTTTATATTCTCAAATACATTTGA

Protein sequence

MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPRERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQGWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRTLSLAHQALFALFLTLFYNFFFYILKYI
Homology
[Close]
BLAST of Cucsat.G4520 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.4e-05
Identity = 69/287 (24.04%), Postives = 114/287 (39.72%), Query Frame = 0 

Query: 68  PRIDYRLIESYDR-DSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEK 127
           P ID+  ++  +R +++ +I +    +GFFQLVNHGIPVEL+  V     +  + +  E+
Sbjct: 4   PVIDFSKLDGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVK-KVSSECYKLREER 63

Query: 128 KVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKKMEWL 187
             G  +  +      +   G+    V  E V+   +D +       P    +F + M+  
Sbjct: 64  FEG-SKPVQLLDTLVKEGDGQRLDNVDWEDVFVLQDDNEW------PSNPPDFEETMKEY 123

Query: 188 TQLTEKLGEKMLGIFLENSG--KIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELENCFK 247
            +   KL EKM+ +  EN G  K    +   G G      +H    G  V       C +
Sbjct: 124 REEIRKLAEKMMEVMDENLGFEKGCIKKAFSGDG------QHPPFFGTKV--SHYPPCPR 183

Query: 248 HDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQGWSG 307
            D+++ L   TD        F               W+   P   AIV+  GD I+  S 
Sbjct: 184 LDLVKGLRAHTDAGGVILL-FQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSN 243

Query: 308 GQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNA 352
           G+YK    R +   H   +   S  N    S+    +P   +++  A
Sbjct: 244 GRYKSAWHRVLATSHGNRRSIASFYNP---SLKATIAPAAGAATEEA 270

[Close]
BLAST of Cucsat.G4520 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.1e-04
Identity = 71/284 (25.00%), Postives = 109/284 (38.38%), Query Frame = 0 

Query: 68  PRIDYRLIESYDRDSVLKILDSMC-SFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEK 127
           P +D  L+   +R + L+ ++  C ++GFF+LVNHG+  EL+  V              K
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTK------DHYK 64

Query: 128 KVGVGRSPE--KAYGFEEYWHGEDESEVSEEFVWSRDEDLK-MEMEVISPIG--YSNFSK 187
           K    R  E   A G ++      +SE+  +  W     L+ +    IS I      + K
Sbjct: 65  KTMEQRFKEMVAAKGLDDV-----QSEI-HDLDWESTFFLRHLPSSNISEIPDLEEEYRK 124

Query: 188 KMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELEN 247
            M+      EKL EK+L +  EN G        L  G +  V+                 
Sbjct: 125 TMKEFAVELEKLAEKLLDLLCENLG--------LEKGYLKKVFYGSKGPNFGTKVSNYPP 184

Query: 248 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQG 307
           C K D+I+ L   +D +      F               WV   P   +IV+ +GD I+ 
Sbjct: 185 CPKPDLIKGLRAHSD-AGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEV 244

Query: 308 WSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPS 346
            + G+YK V+ R I +         S  N    S     SP P+
Sbjct: 245 ITNGKYKSVMHRVIAQSDGTRMSIASFYNPGNDS---FISPAPA 264

[Close]
BLAST of Cucsat.G4520 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.0e-04
Identity = 62/256 (24.22%), Postives = 109/256 (42.58%), Query Frame = 0 

Query: 64  VEHPPRIDYRLIESYDRDSVLKILDSMC-SFGFFQLVNHGIPVELIGAVAGAADAGVFGI 123
           +E+ P I+   +   +RD+ ++++   C ++GFF+LVNHGIP E++  V      G +  
Sbjct: 1   MENFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVE-KLTKGHYKK 60

Query: 124 SPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLK-MEMEVIS--PIGYSNF 183
             E++    +    + G E       ++EV+ +  W     L+ + +  IS  P     +
Sbjct: 61  CMEQRF---KELVASKGLEAV-----QAEVT-DLDWESTFFLRHLPVSNISEVPDLDDEY 120

Query: 184 SKKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDEL 243
            + M    +  EKL E++L +  EN G          +GS    +  K+           
Sbjct: 121 REVMRDFAKRLEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKV--------SNY 180

Query: 244 ENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHI 303
             C K D+I+ L   TD +      F               W+   P   +IVV +GD +
Sbjct: 181 PPCPKPDLIKGLRAHTD-AGGIILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQL 237

Query: 304 QGWSGGQYKHVIGRPI 316
           +  + G+YK V+ R I
Sbjct: 241 EVITNGKYKSVLHRVI 237

[Close]
BLAST of Cucsat.G4520 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 9.7e-04
Identity = 76/294 (25.85%), Postives = 114/294 (38.78%), Query Frame = 0 

Query: 68  PRIDYRLIESYDRDSVLKILDSMC-SFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEK 127
           P +D  LI   +R + L+ ++  C ++GFF+LVNHGI  EL+  V              K
Sbjct: 5   PVVDMDLINGEERAATLEKINDACENWGFFELVNHGISTELLDTVE------KMNKDHYK 64

Query: 128 KVGVGRSPE--KAYGFEEYWHGEDESEVSEEFVWSRDEDLK-MEMEVISPIG--YSNFSK 187
           K    R  E   A G E       +SE+     W     L+ +    IS I     ++ K
Sbjct: 65  KTMEQRFKEMVAAKGLEAV-----QSEI-HYLDWESTFFLRHLPSSNISEIPDLEEDYRK 124

Query: 188 KMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELEN 247
            M+      EKL EK+L +  EN G          +GS    +  K+             
Sbjct: 125 TMKEFAVELEKLAEKLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKV--------SNYPP 184

Query: 248 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQG 307
           C K D+I+ L   TD +      F               W+   P   +IV+ +GD I+ 
Sbjct: 185 CPKPDLIKGLRAHTD-AGGIILLFQDDKVSGLQLLKDGEWMDVPPVHHSIVINLGDQIEV 244

Query: 308 WSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSP 356
            + G+YK ++ R I +         S  N      AF+ SP P+     +  SP
Sbjct: 245 ITNGKYKSIMHRVIAQSDGTRMSIASFYNPG--DDAFI-SPAPALLEEKSEVSP 274

[Close]
BLAST of Cucsat.G4520 vs. NCBI nr
Match: XP_004151051.2 (uncharacterized protein LOC101203918 [Cucumis sativus] >KGN58037.1 hypothetical protein Csa_010946 [Cucumis sativus])

HSP 1 Score: 779 bits (2011), Expect = 3.55e-284
Identity = 387/387 (100.00%), Postives = 387/387 (100.00%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF
Sbjct: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS
Sbjct: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300

Query: 301 GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT 360
           GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT
Sbjct: 301 GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT 360

Query: 361 LSLAHQALFALFLTLFYNFFFYILKYI 387
           LSLAHQALFALFLTLFYNFFFYILKYI
Sbjct: 361 LSLAHQALFALFLTLFYNFFFYILKYI 387

[Close]
BLAST of Cucsat.G4520 vs. NCBI nr
Match: XP_038882380.1 (uncharacterized protein LOC120073645 [Benincasa hispida])

HSP 1 Score: 642 bits (1657), Expect = 3.10e-230
Identity = 325/390 (83.33%), Postives = 353/390 (90.51%), Query Frame = 0 

Query: 3   MMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPRER 62
           MMSPER+A TPFEFRAPPPSPI TSRRSSVTNDEVLTEFLEHSLRVPDLVLP+ IFPR+R
Sbjct: 1   MMSPERSAVTPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPENIFPRQR 60

Query: 63  FVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGI 122
           F+E+PPRID+R+IES DRDSVLKILDSM S GFFQLVNHGIP +LIGAVA AA AGVFGI
Sbjct: 61  FIENPPRIDFRMIESSDRDSVLKILDSMASIGFFQLVNHGIPAQLIGAVAAAAAAGVFGI 120

Query: 123 SPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKK 182
           SPEKKV V RSPEKAYGFEEYWHGEDESE+ EEFVWSRDE LK+EME ISP GYS+FSKK
Sbjct: 121 SPEKKVAVARSPEKAYGFEEYWHGEDESELCEEFVWSRDEGLKVEMEAISPFGYSSFSKK 180

Query: 183 MEWLTQLTEKLGEKMLGIFLENSG-KIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELEN 242
           MEW+TQLTEK+GEK+L I +ENSG K+ + +V+LGHGSVWCVYK K KQ ++ WNDELEN
Sbjct: 181 MEWMTQLTEKVGEKILEILVENSGGKVEKKDVILGHGSVWCVYKQK-KQRMN-WNDELEN 240

Query: 243 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQG 302
           CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFH Y+KRGWVSFVPD+SAIVVTVG+HIQ 
Sbjct: 241 CFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHVYTKRGWVSFVPDESAIVVTVGNHIQA 300

Query: 303 WSGGQYKHVIGRPIYKDHNKEKQSGS----NNNTNGISMAFLFSPTPSSSSSNALKSPNE 362
           WSGGQYKHVIGRPIY+DHNKE++SGS    NNN NGISMAFLFSPT SSSSSN  +  NE
Sbjct: 301 WSGGQYKHVIGRPIYRDHNKEEESGSSSSNNNNNNGISMAFLFSPT-SSSSSNGSEPLNE 360

Query: 363 IRTLSLAHQALFALFLTLFYNFFFYILKYI 387
           IRTLSLAHQALFALFLTL YNFFFYILKYI
Sbjct: 361 IRTLSLAHQALFALFLTLLYNFFFYILKYI 387

[Close]
BLAST of Cucsat.G4520 vs. NCBI nr
Match: XP_008447542.2 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 613 bits (1580), Expect = 4.70e-219
Identity = 307/330 (93.03%), Postives = 314/330 (95.15%), Query Frame = 0 

Query: 58  FPRERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADA 117
           FPRERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADA
Sbjct: 24  FPRERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADA 83

Query: 118 GVFGISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYS 177
           GVFGISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYS
Sbjct: 84  GVFGISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYS 143

Query: 178 NFSKKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWND 237
           NFSKKMEWLTQLTEKLGEK+L IFLENSGKIARNEV  GHGSVWCVYK KIKQ LSVWND
Sbjct: 144 NFSKKMEWLTQLTEKLGEKILEIFLENSGKIARNEVFSGHGSVWCVYKQKIKQRLSVWND 203

Query: 238 ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGD 297
           ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGD
Sbjct: 204 ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGD 263

Query: 298 HIQGWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSS--NALKSP 357
           HIQGWSGGQYKHVIGRPIYKDHNKE+QSGSNNN NGISMAFLFSPT SSSSS  NALKSP
Sbjct: 264 HIQGWSGGQYKHVIGRPIYKDHNKEEQSGSNNNNNGISMAFLFSPTSSSSSSSSNALKSP 323

Query: 358 NEIRTLSLAHQALFALFLTLFYNFFFYILK 385
           NEIRTLSLAHQALFALFLTL YNFFFYILK
Sbjct: 324 NEIRTLSLAHQALFALFLTLLYNFFFYILK 353

[Close]
BLAST of Cucsat.G4520 vs. NCBI nr
Match: KAA0050830.1 (1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 570 bits (1468), Expect = 1.01e-202
Identity = 281/300 (93.67%), Postives = 288/300 (96.00%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPI TSRRSSVTNDEVLTEFLEHSLRVPDLVLP+TIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADAGVF
Sbjct: 61  ERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYSNFS
Sbjct: 121 GISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEK+L IFLENSGKIARNEV  GHGSVWCVYK KIKQ LSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKILEIFLENSGKIARNEVFSGHGSVWCVYKQKIKQRLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGDHIQ 300

[Close]
BLAST of Cucsat.G4520 vs. NCBI nr
Match: TYK08517.1 (1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 569 bits (1467), Expect = 1.44e-202
Identity = 281/300 (93.67%), Postives = 287/300 (95.67%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPI TSRRSSVTNDEVLTEFLEHSLRVPDLVLP+TIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADAGVF
Sbjct: 61  ERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYSNFS
Sbjct: 121 GISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEKML IFLENSGKIARNEV  GHG VWCVYK KIKQ LSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKMLEIFLENSGKIARNEVFSGHGPVWCVYKQKIKQRLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGDHIQ 300

[Close]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match: A0A0A0LBM9 (DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G454120 PE=4 SV=1)

HSP 1 Score: 779 bits (2011), Expect = 1.72e-284
Identity = 387/387 (100.00%), Postives = 387/387 (100.00%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF
Sbjct: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS
Sbjct: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300

Query: 301 GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT 360
           GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT
Sbjct: 301 GWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRT 360

Query: 361 LSLAHQALFALFLTLFYNFFFYILKYI 387
           LSLAHQALFALFLTLFYNFFFYILKYI
Sbjct: 361 LSLAHQALFALFLTLFYNFFFYILKYI 387

[Close]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match: A0A1S3BIA4 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC103489965 PE=4 SV=1)

HSP 1 Score: 613 bits (1580), Expect = 2.27e-219
Identity = 307/330 (93.03%), Postives = 314/330 (95.15%), Query Frame = 0 

Query: 58  FPRERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADA 117
           FPRERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADA
Sbjct: 24  FPRERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADA 83

Query: 118 GVFGISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYS 177
           GVFGISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYS
Sbjct: 84  GVFGISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYS 143

Query: 178 NFSKKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWND 237
           NFSKKMEWLTQLTEKLGEK+L IFLENSGKIARNEV  GHGSVWCVYK KIKQ LSVWND
Sbjct: 144 NFSKKMEWLTQLTEKLGEKILEIFLENSGKIARNEVFSGHGSVWCVYKQKIKQRLSVWND 203

Query: 238 ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGD 297
           ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGD
Sbjct: 204 ELENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGD 263

Query: 298 HIQGWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSS--NALKSP 357
           HIQGWSGGQYKHVIGRPIYKDHNKE+QSGSNNN NGISMAFLFSPT SSSSS  NALKSP
Sbjct: 264 HIQGWSGGQYKHVIGRPIYKDHNKEEQSGSNNNNNGISMAFLFSPTSSSSSSSSNALKSP 323

Query: 358 NEIRTLSLAHQALFALFLTLFYNFFFYILK 385
           NEIRTLSLAHQALFALFLTL YNFFFYILK
Sbjct: 324 NEIRTLSLAHQALFALFLTLLYNFFFYILK 353

[Close]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match: A0A5A7U6N0 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G00900 PE=4 SV=1)

HSP 1 Score: 570 bits (1468), Expect = 4.90e-203
Identity = 281/300 (93.67%), Postives = 288/300 (96.00%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPI TSRRSSVTNDEVLTEFLEHSLRVPDLVLP+TIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADAGVF
Sbjct: 61  ERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYSNFS
Sbjct: 121 GISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEK+L IFLENSGKIARNEV  GHGSVWCVYK KIKQ LSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKILEIFLENSGKIARNEVFSGHGSVWCVYKQKIKQRLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGDHIQ 300

[Close]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match: A0A5D3C991 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00540 PE=4 SV=1)

HSP 1 Score: 569 bits (1467), Expect = 6.96e-203
Identity = 281/300 (93.67%), Postives = 287/300 (95.67%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           MVMMSPERAAATPFEFRAPPPSPI TSRRSSVTNDEVLTEFLEHSLRVPDLVLP+TIFPR
Sbjct: 1   MVMMSPERAAATPFEFRAPPPSPIGTSRRSSVTNDEVLTEFLEHSLRVPDLVLPETIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           ERFVE+PPRID+RLIESYDRDSVLKILDSM SFGFFQLVNHGIPVELIGAVA AADAGVF
Sbjct: 61  ERFVENPPRIDFRLIESYDRDSVLKILDSMRSFGFFQLVNHGIPVELIGAVAAAADAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GISPEKKV VGRSPEKAYGFEE WHGEDESEVSEEFVW+RDEDLKMEME ISPIGYSNFS
Sbjct: 121 GISPEKKVAVGRSPEKAYGFEELWHGEDESEVSEEFVWNRDEDLKMEMEAISPIGYSNFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
           KKMEWLTQLTEKLGEKML IFLENSGKIARNEV  GHG VWCVYK KIKQ LSVWNDELE
Sbjct: 181 KKMEWLTQLTEKLGEKMLEIFLENSGKIARNEVFSGHGPVWCVYKQKIKQRLSVWNDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQ 300
           NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFV D+SA+VVTVGDHIQ
Sbjct: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVADESAVVVTVGDHIQ 300

[Close]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match: A0A6J1GAF1 (uncharacterized protein LOC111452244 OS=Cucurbita moschata OX=3662 GN=LOC111452244 PE=4 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 7.10e-179
Identity = 273/390 (70.00%), Postives = 305/390 (78.21%), Query Frame = 0 

Query: 1   MVMMSPERAAATPFEFRAPPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPR 60
           M MMSPE  AATPF+FRAPPPSPI TSRRSSVTND+VLT+FLEHSLRVP+LVLP+ IFPR
Sbjct: 1   MGMMSPELTAATPFDFRAPPPSPINTSRRSSVTNDDVLTDFLEHSLRVPNLVLPENIFPR 60

Query: 61  ERFVEHPPRIDYRLIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVF 120
           +RF+++PPRID+R IES D DSV++ILDSM S G FQL NHGIP+ELIGAVA AA AGVF
Sbjct: 61  QRFIQNPPRIDFRSIESSDHDSVVRILDSMGSIGCFQLGNHGIPMELIGAVASAAAAGVF 120

Query: 121 GISPEKKVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFS 180
           GIS EKK  V RSPEK YGFEEYW GEDESEVSEEFVWSRDE L+ EME IS  GYS+FS
Sbjct: 121 GISREKKTVVTRSPEKGYGFEEYWDGEDESEVSEEFVWSRDEGLRKEMEGISGFGYSDFS 180

Query: 181 KKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELE 240
            KME LTQ+TEK+GEK+L IF EN GK+A  EV    GSVWCV+K K +Q     NDELE
Sbjct: 181 NKMEALTQVTEKVGEKILEIFRENCGKVAEKEVGWRQGSVWCVHKQKQRQ-----NDELE 240

Query: 241 NCFKHDVIRMLIRGTDFSHAFCFHFCHGSSP-----LFHAYSKRGWVSFVPDDSAIVVTV 300
           NC KHDV+RMLIRG+D SHA CFHFCHGSS      +FH YSK+GWV FVP++SAI+VTV
Sbjct: 241 NCLKHDVMRMLIRGSDLSHALCFHFCHGSSSSSSSCVFHVYSKKGWVCFVPEESAIMVTV 300

Query: 301 GDHIQGWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSP 360
           GD IQ WSGGQYKHVIGRPIYK    E++  + NN  GISMAFLF+PT S S        
Sbjct: 301 GDQIQAWSGGQYKHVIGRPIYK----EEKGNNINNGIGISMAFLFAPTSSPS-------- 360

Query: 361 NEIRTLSLAHQALFALFLTLFYNFFFYILK 385
           N   TLSL HQALFALFL L YN F Y L 
Sbjct: 361 NVPPTLSLPHQALFALFLILLYNAFLYFLN 373

[Close]
BLAST of Cucsat.G4520 vs. TAIR 10
Match: AT1G79760.1 (downstream target of AGL15-4 )

HSP 1 Score: 183.7 bits (465), Expect = 2.8e-46
Identity = 136/371 (36.66%), Postives = 195/371 (52.56%), Query Frame = 0 

Query: 15  EFRAPPPSPIRTSRRSSVT-NDEVLTEFLEHSLRVPDLVLPDTIFPRERFVEHPPRIDYR 74
           +FRAPPPSP+ +SRR+S T N++VL+EFL+   RVP+LVLPD +FP+  F+ +PP  D+ 
Sbjct: 7   DFRAPPPSPVASSRRASFTANEDVLSEFLDKCGRVPNLVLPDKVFPKHTFLLNPPTFDFH 66

Query: 75  LIESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEKKVGVGRS 134
            +     DS+  +LD++ + G FQLVNHG+P  ++ A   A D  +F             
Sbjct: 67  RL-----DSLSALLDAIATIGCFQLVNHGVPEAMVKA---AMDKTIF------------- 126

Query: 135 PEKAYGFEEYWHGEDESEVSEEFVWSRDEDLKMEMEVISPIGYSNFSKKMEWLTQLTEKL 194
                      H E     +EEFV+++D                N        + L E +
Sbjct: 127 -----------HDE-----TEEFVFNKD---------------INADDHTACYSDLRELM 186

Query: 195 GEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVW---NDELENCFKHDVIRM 254
           GE       E  GK  R ++  G  S         K+G+ V       ++N  K + IRM
Sbjct: 187 GE------AERIGKAVREKLGAGGRSQ--------KEGVGVCYVKRHNIKNESKEEAIRM 246

Query: 255 LIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQGWSGGQYKHV 314
           L+RG D  H+ C +FCH     FH YSKRGWVSF P   A++VT+GD  QGWS G++K V
Sbjct: 247 LLRGYDERHSLCLNFCHAE---FHVYSKRGWVSFSPRPDAVIVTIGD--QGWS-GRFKGV 296

Query: 315 IGRP-IYKDHNKEKQSGSNNNTNGISMAFLF-SPTPSSSSSNALKSPNEIRTLSLAHQAL 374
           +GRP +YK         S+ + N IS++FL+ S T   ++S  +    + +T+SL  Q L
Sbjct: 307 VGRPLLYK---------SDLHHNLISISFLYTSTTTLHNTSRKITKRTKKKTISLFQQLL 296

Query: 375 FALFLTLFYNF 380
           FALFLTL + F
Sbjct: 367 FALFLTLLFPF 296

[Close]
BLAST of Cucsat.G4520 vs. TAIR 10
Match: AT2G38500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 9.9e-20
Identity = 105/370 (28.38%), Postives = 159/370 (42.97%), Query Frame = 0 

Query: 19  PPPSPIRTSRRSSVTNDEVLTEFLEHSLRVPDLVLPDTIFPRERFVEH---PPRIDYRLI 78
           PPPSPI  +R S     E+LTE +E S++VP+L LP++    E        P  ID+RL+
Sbjct: 20  PPPSPIPRARGSRCAASEILTEIIERSIQVPELTLPESHSGGESCGSRHLIPAEIDFRLL 79

Query: 79  ESYDRDSVLKILDSMCSFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEKKVGVGRSPE 138
            S    SV +++ S   FG F++  HGI  E + ++       VFG+   +  G  RS  
Sbjct: 80  ASRREGSVDRLVRSAREFGAFRVSYHGISGEELRSLV-RESGRVFGVLEGRDTGFHRSVV 139

Query: 139 KAYGFEEYWHGEDESEVSEEFVWSRDEDLKME--MEVISPIGYSNFSKKMEWLTQLTEKL 198
                             +E VW R    +ME   E I P  Y  FS++ME +    E +
Sbjct: 140 GN---------------RDEIVWVRSWKERMEWAREYIGPERYRCFSQEMENVADKLEDI 199

Query: 199 GEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELENCFKHDVIRMLIR 258
             K+  I +ENS +    ++  G            +  LSV+    EN  +        R
Sbjct: 200 ARKLGQIMVENSRRPNDKKIQRG------------ESVLSVYRYNHENVTEQSPPLPKER 259

Query: 259 GTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQGWSGGQYKHVIGR 318
             +  H          +  F   S +G +SF  D   I+VT G  ++ WS G++K   G 
Sbjct: 260 TEEMLHYTLSLHLPAKNCEFRVNSGKGPLSFHADPDTILVTFGRQLEEWSLGEFKCRQGE 319

Query: 319 PIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNALKSPNEIRTLSLAHQALFALFL 378
            IY  H     S ++ +     M+   S T  +++S         +T SL HQ +F  FL
Sbjct: 320 IIY--HPDAYGSPTSFSVELKCMSLFLSHTSIATTS---------KTFSLTHQ-IFTAFL 349

Query: 379 TLFYNFFFYI 384
            LF+   F+I
Sbjct: 380 LLFFFQCFWI 349

[Close]
BLAST of Cucsat.G4520 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 45.4 bits (106), Expect = 1.2e-04
Identity = 62/289 (21.45%), Postives = 117/289 (40.48%), Query Frame = 0 

Query: 68  PRIDYRLIESYDRDSVLKILDSMC-SFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEK 127
           P +D   +   +RD  + +++  C ++GFF++VNHG+P +L+  +        +    E+
Sbjct: 8   PVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIE-KMTKDHYKTCQEQ 67

Query: 128 KVGVGRSPEKAYGFEEYWHGEDESEVSEEFVWSRDEDLK----MEMEVISPIGYSNFSKK 187
           K        K+ G +       E+EV E+  W     ++      +  IS +    +   
Sbjct: 68  KF---NDMLKSKGLDNL-----ETEV-EDVDWESTFYVRHLPQSNLNDISDVS-DEYRTA 127

Query: 188 MEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDELENC 247
           M+   +  E L E +L +  EN G        + HG+    +  K+             C
Sbjct: 128 MKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKV--------SNYPPC 187

Query: 248 FKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHIQGW 307
            K ++I+ L   TD +      F               W+   P + +IV+ +GD ++  
Sbjct: 188 PKPEMIKGLRAHTD-AGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVI 247

Query: 308 SGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNA 352
           + G+YK V+ R +       +Q G     N +S+A  ++P   +  S A
Sbjct: 248 TNGKYKSVLHRVV------TQQEG-----NRMSVASFYNPGSDAEISPA 265

[Close]
BLAST of Cucsat.G4520 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 2.6e-04
Identity = 63/292 (21.58%), Postives = 110/292 (37.67%), Query Frame = 0 

Query: 68  PRIDYRLIESYDRDSVLKILDSMC-SFGFFQLVNHGIPVELIGAVAGAADAGVFGISPEK 127
           P ID   +   +RD  + ++D  C ++GFF+LVNHG+P +L+  +             E+
Sbjct: 8   PVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNI-------------ER 67

Query: 128 KVGVGRSPEKAYGFEEYWHGED----ESEVSEEFVWSRD---EDLKMEMEVISPIGYSNF 187
                        F+E    +     E+EV E+  W        L        P   + +
Sbjct: 68  MTKEHYKKHMEQKFKEMLRSKGLDTLETEV-EDVDWESTFYLHHLPQSNLYDIPDMSNEY 127

Query: 188 SKKMEWLTQLTEKLGEKMLGIFLENSGKIARNEVVLGHGSVWCVYKHKIKQGLSVWNDEL 247
              M+   +  E L E++L +  EN G        + HG+    +  K+           
Sbjct: 128 RLAMKDFGKRLEILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKL--------SNY 187

Query: 248 ENCFKHDVIRMLIRGTDFSHAFCFHFCHGSSPLFHAYSKRGWVSFVPDDSAIVVTVGDHI 307
             C K ++I+ L   TD +      F               WV   P   +IV+ +GD +
Sbjct: 188 PPCPKPEMIKGLRAHTD-AGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQL 247

Query: 308 QGWSGGQYKHVIGRPIYKDHNKEKQSGSNNNTNGISMAFLFSPTPSSSSSNA 352
           +  + G+YK V+ R +           +    N +S+A  ++P   +  S A
Sbjct: 248 EVITNGKYKSVMHRVM-----------TQKEGNRMSIASFYNPGSDAEISPA 265

The following BLAST results are available for this feature:
BLAST of Cucsat.G4520 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FR991.4e-0524.041-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=... [more]
Q009851.1e-0425.001-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q085072.0e-0424.221-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
O488829.7e-0425.851-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 P... [more]
BLAST of Cucsat.G4520 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004151051.23.55e-284100.00uncharacterized protein LOC101203918 [Cucumis sativus] >KGN58037.1 hypothetical ... [more]
XP_038882380.13.10e-23083.33uncharacterized protein LOC120073645 [Benincasa hispida][more]
XP_008447542.24.70e-21993.03PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo][more]
KAA0050830.11.01e-20293.671-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa][more]
TYK08517.11.44e-20293.671-aminocyclopropane-1-carboxylate oxidase [Cucumis melo var. makuwa][more]
BLAST of Cucsat.G4520 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LBM91.72e-284100.00DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G454120 PE=4... [more]
A0A1S3BIA42.27e-21993.031-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC10348996... [more]
A0A5A7U6N04.90e-20393.671-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5D3C9916.96e-20393.671-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1GAF17.10e-17970.00uncharacterized protein LOC111452244 OS=Cucurbita moschata OX=3662 GN=LOC1114522... [more]
BLAST of Cucsat.G4520 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G79760.12.8e-4636.66downstream target of AGL15-4 [more]
AT2G38500.19.9e-2028.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G62380.11.2e-0421.45ACC oxidase 2 [more]
AT1G12010.12.6e-0421.582-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 5..94
e-value: 3.8E-7
score: 31.6
NoneNo IPR availablePANTHERPTHR34945:SF5DOWNSTREAM TARGET OF AGL15-4coord: 8..112
NoneNo IPR availablePANTHERPTHR349452-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 8..112
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..74

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4520.T1Cucsat.G4520.T1mRNA
Cucsat.G4520.T2Cucsat.G4520.T2mRNA
Cucsat.G4520.T3Cucsat.G4520.T3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051213 dioxygenase activity