Cucsat.G4365 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4365
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Gibberellin 2-beta-dioxygenase
Location	ctg1173: 757108 .. 761036 (-)
RNA-Seq Expression	Cucsat.G4365
Synteny	Cucsat.G4365

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGACTTCATTTACTTAAGGTATCAATTCTATCTATCAACATTCATCCAGCTATATACTTCTCTTAAGCATTATCATTATTACATTGTTAGTTTCAATACTTAAGAACACTGTGCCTATATCAAACTATATGGCCTTTTAGACTTGAAAAAGTTTTAGATTAATCAGACTATGCTTTTACTTGTTAATAATATCACATTTACAGAAGTTACCTAATCATCATATCCCATTCATATTTTAGTTTAAAGTTTTAGGAACATGATTGAATGATTTTATAAAGTTCGATTTAAAATCTAATTGTTCAAAGACATCTAGCGAACTTGAAGAGTCTAGCTCTTGTAGACATTTTCACAAACAGTTAGAGCGCAAAAGGTAACGACACTTCATTCTTCCGCAGATATACTAAATTAGCAAACAATAAGAAAAGCCCATTAATGAATTGACTGACCTTACATCATGGAACGGCAGGGAAGTGATGGAGGAAGTGGCAAGCGCGATGTCGAAGGTAGCAAGAAAATTGGCAGGAGTTTTAGTGGAGAGTATGGGGCAGAGGAAGGAATTATTAGAGGATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATTTGTCCATTTTCCGGCGAAGTTTCAGGGCTGGTGCCACACACGGACAGCGATTTTCTTACAATACTTCATCAAGATTCCGGCGGTGGTCTTCAGGTGATGAAAGGATCGCAATGGTTAGCTGTGAAACCCAATCCACAAGCCCTTGTTGTCAACATTGGAGACCTTCTTCAGGTAAACACTCCAACTCCAATTTCTTTTCATCCAATCATTTACCATTCACTCTTTTTTCAGTTTCCATATTACCTAATCTATTGACATTAATATTGAGAAATTCTTTATGAATATTGTATAAACAAATTTACCTTAAAAAAAAATTAAGATAGATTTTAGTGATCAAAAGTCTATGACTTGAACACTTGAATGTGGTGGACCAAATTTTTAACATATTCTCTAGAACAACACAAAGTATTTTATGTTAAATATGTGTCAAGTGAACATACTTCAAACATACGAATTTATGAACAGCTAAGAAATCTGTATTTTGAATTTCATCTTTTCAATATATATCTTCATTTCAATTATGCAATCTAAGATAGTATAACTATTTATTATTACGATAGTCATTATTACCAATACCCCAAAGTGAACACTTTGGAGAAAAGAAAGAATGAACATCTCAACCTCTTACCACTAGAGAATATAAAATAATGAATAAATCAATGAGAGGATATAAATAGAAATGAATCATTTAATACACGAAATCCAATTATGAATTTAAATAAAAAGAAAAACCATCTGACTTCTTTTTTTTCTACTTAATAACAAGGAAGTGACTTTCTACTTACCAATTCCTAAGTTTGAGATTATTTGTATTAGAGTCATCTAAATTGACTTTAAAATACTTTTAAAAGAACTCATTTTTTTATATATAAAAATATATTTTAAAACTATTTTTAAAAAGTCAATTTTTTTAAATAGCACTTGAAAATGAATTTCAATAAACAGATTTTAAAATGTTATATATTTAATCCAAAAGTTTTTTAATTTCAGTTTAGTCCATAATTTTCTTGATATTATCCTAATTAAAGATTCAGAAGATCAAATTTTATGGATTTAAAGTAGAGATATATACAATGCTTTGAATCCAATTAGTAAGTATGAAATTATAAAATTGTACTTGCTATATCAAAATTTCTAAATCAAAATTTCTAAATTTGTAATACTTTTATCCAATCAAGGGGACTCAAAAAATAAAAATAAAAAAAAAATGAACATCCAACCTATAGAAAAATGTTATGATTCAACTTAATTAAACTGTCGGCACAAATTTGCAAAAGTTCAGTCTAATAATTATTATTTATTATTATCTTTTTTTATTGTTATTTAATTTGTCTGGTATTTGAAAAGGACACGTCTTTCTCCATCAAAGTATGTATCAATTTTCAATTCTATTTTTTTATATTTCAAAAGTTTTATTTTGTCTCCATTGTTATAAGTTTTTTTAAATCAAACAATATAGTCAATAATAATCATTGATAGTGTTGTTACAAAGAACCCAATATATATCTTTTTAGTATAATGGTACCACCTAATGGGTTTTCCAATTAAATAAGAGTTGATTTAGTGAGGTTGGACATCAACTTATAATATTATACCATAAAAATACATTGGTAGGGAATAAATTATAATAAAATATTTCTTTTGAAAAAAAAAAAGAACTTTAAACTTCTTTCTATCTATATGTCCATCTCTCTGAATATATACAAAAAGTTGGACTTCAACATTTTGAAGTCAGTATATAATTTGGCTAGCTAACATTTTCTTTTTAATACTTCTGATAATGATTTAGTCCAATAATTTGGTACCTAATATTAAATCCATTGCAATCAATACTCAAATAATTAAACCACCTTTTTGTTTTGGACTATAGGTTTTCTTACTTCATTCATTTGCAATATTCCATTCCTTTTTTAAGGCTGTTTTTTCCATAGAATATTTGGGTGAAAATGCTATCTCAACTAATCAATCACTATACTTCTCAAAATACATGCAACACATCATAACAAATAAAAGTATCAGAACCAAAATAAATTATTATAAGAGCAAAAAAATGTCATTCATAATGTTTTAGAATTATACAATTTGGTCGACTTTCATTATATATATCTTTGCCCCCTCCTTATGGAAGTTCTTGACCCATATATGTGTGATATCTCTCAACTGATTCCCTTGGTTAGTCCACCCGGGTTGGATCTACTGGAACAGAACCTTCATCTAAACCAACCTATTATTACAACATAAACTAACTTTAGTTATTTTCTCATTGTTTTCATCTTTATTTTATTTCTGGTTGAAATATATAAATCTGGGAATGCAGAAAGAAATGTTGAAGAATATGATAGAGCATCATTAACTTAATGATTTTTTCTTCAAAGCTGAGTGAGACTAGATTTTGGAATCAAACCACAATATATTCTAGTCTCACATAACTACATCATTGAAGACATTTGCAATATATTAAACATACCATACATAGGGTGGTGTCTAAAAATGGTGCAAAAGCTTTTTTTTCTTTTTTTTCTTTTTTTTTTTCTAGACCTTAACTCAGAGATCCTTCAAATGTAATGTATGGGATTGAATCTTTGACCTCTTAGGTTAAAAACATATACCTTTAGACCAGTTGAGTTATGTTAAGAATGGCAAAAAACTAGTTCCATATAAAACACAGTAAAAAGAGCCTCTAAGATCTGATTCGCATATCCTACAAATTTCAAACAAAAACAAGCTTCACAACCCCACTGTGTTCCTCATCCCAAAAGAAAATCATCCTCAAACTCAAACAGGAAAATTCCTTAGACAGTAGGATGAGTTTTGGGATTCCTTTCAAAAAGTATTTTTCCTTTTTAAGAGAAATGTTTATTTAACTAATACATCATCTTATTTGATGTTAGTATTTATCAAACTAGTTTTGATTGCCAAATATTATAAGAAGTACAAAAGATCACGGACTAATAAGTTAAGCTGCAGGCCTGGAGCAATGATACGTACAAGAGTGCAAAGCATAGGGTCGTGGCTAACCCGATGAGGGAAAGATATTCGATAGCATACTTCCTCTGCCCTTCTTATCACTCTCAAATTGGGAGTTGCAGAGAGCCTTCACCTTATAGACACTTCACGTTTGCTGAGTACAGAAAACAAGTCAGAGATGATGTGGAAAAAACTGGCTACAAAATAGGACTTTCAAATTTTCGGCTTTAAATCACAGCAATAACAAATAAAAGAACTACTATACGGAAGACCATTACTATAGCAAATACCAATTCAACTGAAGCACATATAGAATGAAATTATAGTTGCGTCGACAACTCAATGTTACATAAAGCAAAAAAGTCTTTGAAGAAATCTAATGTGCACAAAACTGTTCCTGGTTTAACCTTGACTCAAATAGCTACTGATACAGGATATATATCTAATTTCACTGCGTAAAATTCCCAGAAAGAATTCTGACCATGTCAAGAACATGTTCCATAAGCTTAAAAACATAGCTACAATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTTTTTAAAGAAAACAAGAAATATAAAGTCAATAAGCTTATGTATGCTTAAAAAGAATATTCAATGCATTTCACTTCAAAATTGATGAAGTTTTCCTTATACTACTAATTTGATCCTACTATGTTTACAAGTGTTTGGCAACCGTGATTAACAAAACATTCAACTGAGACTAAAGACAAGACCCTCAATCAATAGCCATGTCCATAGGTCGTGGCTTTCTATAGTTTTGGCTAAAGAAGCAGACATATATTGCACCTTTTTGGCCTATAAAATCCACATTTCTTATCATTTTTATACCAACAAGAAATATAAAGTCAATAGGCTTAAGTATGCTTTAAAAAATTATATTTGATGCAAAGAAATATAAAGTCAATAGGCTTAAGTATGCTTAAAAAAATATATTTGATGCATTTCAATTTTAAAATTTATTAATTTTAGTAGTTTACCTCATGATTCTATTTTGTATGTTTATTATGTTTACCAATTGTGTGACATATAACTAACAAAAGTTGGGTTCATATTTAATTTGCACACCTAATGTATGACATGTACCTGTTGTGCTAACATGTGCCAACTTAAATGTTCCTTTTTTCTTCTTTTTTTTTATATCCGTGAGTGTCCAAGCCATCTTACACACACCTCGACTAATCTCACGGGACACATTCGGATGTCAAGGAAATTCGTAAGAAATTAATTCTTAGGTAGGTGGCCACAATGGATTGAACCCATGACCTTTTAGTTATTTGAAACTATGTCTCTCTTTTTACCACTAGGTCAACCCATGATGATTCTATCCGAAATATTTCAAACACAAATCCATTGCTCAACTTGAAGTGTGCACACTTTGTAGAATTTAACCATTCAACCATGAGTTATTATGTGTACCTTGTCTCCCTATGATAACCAGCCTCACTCCCCTTTCCTCTCCGGTCTTTGGAAGATCAAACTTCAGTCACAGATGTGCTGTTTTTAAAGAAAAAAATCATAATTGTTAAAAATAATTGTACGGAGGGTATTTGTGTAATCTTTATATTTGGGTTTATGTCCTGGTTTATTGTATTCATGTATTTATTATACTCATGTGAGTAATTCTCTCACGGCTAAATTCAACGTTGTTTTCTCATACTAGAAGTTTTAGTCCAAGTGAAAGTTTGTAGGAATTTATGTCTTAAAACTCGTAGTTTGTAGTTAAAATTATATTTTATTCAATAAAGTTGTTATTGAGAACTTATCAGTGAAAATTGAATGTTATAATCTTGAATCCAATAAACTAAGGTCCCGAGGCTATCTAGTGTAAACTTGAACTTGAGACATACATGTGGATCAAGTTCGAGTATATAGCCCAAACGGTCTACAGTGTATGAATAAAGTTAGATACCTTATTCAGGAGACACTATAAATGTTGTCCGCTTTGTAGTTAGTAGAAACAATGTGATCCTAAATCATTCATGTAAAAACATGAAAATGGGGACATCCTATGTAAAGAGTTTACATAAGACTAGAATCATGAAATAGTTAATTTTAAGTTATAACAATGTTGATTATATAAAACTGACTATTTCGATTATTGATGACCTAAGGTAACTTAGTCTTAATCTTGAACTAACTATGAACTTTTGTTCAAATGGAATTATCCTTAAAAATCTGCAAAGGTGAGGACAGCTCAACAACGCTGGCTCGATAAGCCTTCCATTTCAAGGGTAAGACCAGGTGAAGGACTAGAGACATAGGGTGCAAGATGGAATTTCCTCCTACCCACTTTAGGGTTAGTAGATAGGTTGTTCTCTTAAAGACTGAATCCAAATCTTGAACAAGGGGCCCCACCCTCTCATTGGCCCTAGAGGGATTCGATTTACAGTTTAAACCATAAACCAATAGTACAATAATGGATCAGTGGGATTTAAGGAGCAAGATGTAGTCTCGAGAGTAAATCAATATTTTAACCAGTCGAAGTTATGAACTATTTGTAAATGATTAACTTACTGATCATGGTTATATCTATAGGGAGGAGAGTGCAACTATAGAACTTTAGTGGAATGACCCGTTAGTTAACGAATGTTGATTAGCTCGATCTAAAAAAGTTTAATCAGTTAATCTCGGATCGCTGGAACTCATGATCTATAGGTCCATTAAGTTCCCCTATTAGCTTATATGGAAGAAACTGATAGACCTCCAACACTATATCAAAATAGTAGGAGAGGGAAGAAGGGTAGTGGCACGGCACGTGTGTAGAGTGACACGTGTGAGTTAGGTTAGTATAAATAAAGACCAAATGTGGGGCGGGAAAGTTAGTTAGAAACTTTGAAGGAGTAGGTCTTCTTCCTTGAGAGATAGGATAACAGGAGAGATAGGATAACAGGAGAGAGTTTTCAGTTTTAGTGTGTGATCGCATTAGTGTGTGTAACCTAGATTCAGTTTGAATCTATTATCAATAAAAGTAGAATTTTATTCTCTTAGTTTCTGGATTCCATCAGAAACTTAGAACAATATGATAGAATAATTCAAATTAGGTCAAGAGAGAGAAACTAACGAATATATGTGATATAACCGTCGGTTATCAGTTTGAGATAGAACCTTATGTTTAAATATGATTTAAATATTTAAAATATGAATACGAATTCATATTCGAAAGCTTGGAATTGATGGAAATGGTCAAAGTTGTAAAAAGTTGAAATGTTGACTTTTGACTTTGATAAGTCAAACTTTGATCGGCTTTACATTCAAATGTGATTTGAATTTCGAAAAAATGAAAGCAGATTCATGCTAGTGAGGTTGGAACTTTTTCAGGAGGATACTCAGGTACGATCATCATCTTCCCACCACTCTTCAAACTCAACCAAATAGCTTTCAAATTAAGTTTTCTTTCTTCGCTCTAATGACTAGTAATCTTCTCTTTCTGCCCATCTTTGTTGAGAATCCTTGATCATTTGTCTTATTTCTCTAATTTCTATATGTATCTCTCAGAATACGTTAATTCCATTATCAAGATCCATTACTAAAATTGAAGTTCCAAGATGAATGGCTCTGAAACAAATTTGTTAGGATATTGTTAGATACCTAGATTAATATAGGGTAGGGGTATAAAGGTAATTAGATTTGTTGGTAGTTAGTTGGTTATAAATAGGAAGTTGGGGAGGGAAGAAAGGGGAAAGAATTTTGGTGAGATATTGGGCTGCAACATTCCTTGAAAGAGAGGAAGGGCAGAGGGTTAGGGTTCTTTTACTTGTTTGTTCGTTTACTTCTTTCATATTGTAATTTCTGTTTGAGATATCAATAAAATAGAATCACTATATCGGTGTTCTATCAGATACTTCCTAACAAGAACAAGATCTTAATTTATCTTTGTGATGTGTGTAAGCTTGAACAATTTGATTTTTGGCAGAGAGACTACCAGACTATTTTTCTTGATAATATTGGTGAATGTTTAAGTGTTTTTTCCTTTCTCAAAATGCATTTGGTAAATTTGAACATTTTAAATGCTTGTCAAGGCAAGTCCTTCTGAAATTAAAGGGAGCTGGGAGTTTTATGAAAAAAAAATGGTAGTTAGAAAGAAGATAAAGAAGATCGCCCCAGTTTTGGTTTTGCTACAAGTATCAAAAGTAAAGTTATATTCCAAATTCCTCAATTGAGAAGATATTTTGCTCTTTTGTTGCTTATGAACCTTTTGTAAAAAAATTTATGGATCTCATTTGAATATGGACTGTATGATCATAAAGAAACGATATATTTGACTGGAACTTTTTGTTTTATCAAAGTTCATTGTGGAGCCTTGATCGTTTGGAGGTAATTAAGTTTAGCAAGACGATTTCCAGCAGTCAATATGACTTGCGAAAGAAGAAGATGTTTAGCTGTTTATCAGAAAGTAGCTTCCTGGAACTTTTGTCTCAAGTAATTTTGAAGGTTAAAGAAGTCGGTGAATTTTAGATTCCTTTAGGGCTTTAGTAATAATAGAAAGAAAGGGTTATAATTGATCAAAGATAGTGGCCGCTGACTCCTGTTGGAAAGTTTTTGAGTTATTTCCTTCCTCTAAATAAAAGTTGTGGATCTTTGTTTGGAATTTCGTTTTTGGTCATTGTTTATCCCCTTCAATCTGCTCTTATGCTCTCCCACTAGTGACTCCTTATAGCTCCTATTTGCTTTTTCTCAACTGTTTTGTATTATTGTCAGAATTGTGCTTGGGAAGATTCCAGAGATATTTTCAGTGCAAGCTTCTTAGCATAAGTCCCTAG

Coding sequence (CDS)

TCTGAAGCTTTTCACATTCCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGACTTCATTTACTTAAGGGAAGTGATGGAGGAAGTGGCAAGCGCGATGTCGAAGGTAGCAAGAAAATTGGCAGGAGTTTTAGTGGAGAGTATGGGGCAGAGGAAGGAATTATTAGAGGATATTTGCGACGAAAGCACATGCTTCCTCCGGTTGAATCACTACCCAATTTGTCCATTTTCCGGCGAAGTTTCAGGGCTGGTGCCACACACGGACAGCGATTTTCTTACAATACTTCATCAAGATTCCGGCGGTGGTCTTCAGGTGATGAAAGGATCGCAATGGTTAGCTGTGAAACCCAATCCACAAGCCCTTGTTGTCAACATTGGAGACCTTCTTCAGAATTGTGCTTGGGAAGATTCCAGAGATATTTTCAGTGCAAGCTTCTTAGCATAA

Protein sequence

SEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQNCAWEDSRDIFSASFLA

Homology

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BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 6.6e-77
Identity = 138/235 (58.72%), Postives = 168/235 (71.49%), Query Frame = 0


Query: 2   KLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAAC-YGDFIYLR 61
           +LF +PFE K  +G+L+ SYRWG PTAT     SWSEAFH+PL  +S A C +GD   LR
Sbjct: 104 RLFRLPFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLR 163

Query: 62  EVMEEVASAMSKVARKLAGVLVESMGQR-------KELLEDICDESTCFLRLNHYPICPF 121
            VM+EVA AMS+VA  +A  L E +  R              CDE+TCFLRLN YP CPF
Sbjct: 164 GVMQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPF 223

Query: 122 SGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDT 181
           + +  GLVPHTDSDFLT+L QD  GGL +MK S+W+AV+P P ALVVNIGDL QAWSN+ 
Sbjct: 224 AADTFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNR 283

Query: 182 YKSAKHRVVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 229
           YKS +H+VVAN   +R S+AYFLCPSY S +G+C EPSPYR FTF EYRK+V++D
Sbjct: 284 YKSVEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQED 338

[Close]

BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 1.8e-66
Identity = 121/229 (52.84%), Postives = 158/229 (69.00%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILD-NSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYL 60
           I++F  PF+KK  S      SYRWGTP+AT   Q SWSEAFH+P+T +S+     DF  L
Sbjct: 95  IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTTL 154

Query: 61  REVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSG 120
              ME+ AS    +A  LA VL E  GQ     ++ C  +TC+LR+N YP CP   EV G
Sbjct: 155 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 214

Query: 121 LVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKH 180
           L+PHTDSDFLTIL+QD  GGLQ++K ++W+AVKPNP+AL++NIGDL QAWSN  YKS +H
Sbjct: 215 LMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEH 274

Query: 181 RVVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 229
           RV+ NP  ER+S AYF+CPSY + I    +   YR+F+F E+R+QV++D
Sbjct: 275 RVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQED 320

[Close]

BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.1e-58
Identity = 120/241 (49.79%), Postives = 159/241 (65.98%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTS---GILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFI 60
           + +F  PF++K T    G    SYRWGTPTA    Q SWSEA+HIP+T    +      I
Sbjct: 91  VAVFRRPFQEKVTERLLGFSPESYRWGTPTAKCLEQLSWSEAYHIPMTTPRPSTS----I 150

Query: 61  YLREVMEEVASAMSKVARKLAGVLVESM---GQRKELLEDICDESTCFLRLNHYPICPFS 120
             R V+EEV+ AM ++A+KLA +L+  +   G+ + ++     E TCFLRLN YP C  +
Sbjct: 151 RARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGETMV--TTREETCFLRLNRYPPCAMA 210

Query: 121 GEVSGLVPHTDSDFLTILH--QDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSND 180
               GL PHTDSD LTI+H  QD+ GGLQ++KG +W+AVKP+P  L+VN+GDLLQAWSND
Sbjct: 211 MGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKPSPSTLIVNVGDLLQAWSND 270

Query: 181 TYKSAKHRVVANPMRERYSIAYFLCPSYHSQIGSCR-----EPSPYRHFTFAEYRKQVRD 229
            YKS +HRV+AN   ER+S+A+FLCPSYH+ I         + + YR FTF EYRKQ+ +
Sbjct: 271 VYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAHYRSFTFGEYRKQIME 325

[Close]

BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.9e-48
Identity = 103/230 (44.78%), Postives = 139/230 (60.43%), Query Frame = 0


Query: 2   KLFGVPFEKKFT---SGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIY 61
           KLF  PF  K     S +  NSYRWG P+AT P Q+S SEAFHI L++VS  +   D   
Sbjct: 94  KLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNN 153

Query: 62  LREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVS 121
           LR ++E     +++VA+ +  +L + +    E  E+I +    FLRLN Y    F  EV 
Sbjct: 154 LRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVF 213

Query: 122 GLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAK 181
           GLVPHTD+ FLTIL QD  GGL++    QW++VKP  +AL VNIGD+ QA SN  Y+S +
Sbjct: 214 GLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVR 273

Query: 182 HRVVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 229
           HRV++    ER SIA+F+CP   ++I     P  YR F+F EY++Q   D
Sbjct: 274 HRVISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHD 321

[Close]

BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-28
Identity = 64/149 (42.95%), Postives = 87/149 (58.39%), Query Frame = 0


Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           +V +E   AMS ++ K+  +L  S+G +++   +  +E+   +RLN+YP C       G 
Sbjct: 185 KVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGT 244

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
            PH D   LTILHQD   GLQV   +QW +++PNP+A VVNIGD   A SND YKS  HR
Sbjct: 245 GPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHR 304

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCRE 210
            V N   ER S+A+FLCP     +   RE
Sbjct: 305 AVVNSESERKSLAFFLCPKKDRVVTPPRE 333

[Close]

BLAST of Cucsat.G4365 vs. NCBI nr
Match: KGN49660.1 (hypothetical protein Csa_018434 [Cucumis sativus])

HSP 1 Score: 474 bits (1220), Expect = 3.92e-167
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR
Sbjct: 102 IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 161

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL
Sbjct: 162 EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 221

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR
Sbjct: 222 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 281

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD
Sbjct: 282 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 329

[Close]

BLAST of Cucsat.G4365 vs. NCBI nr
Match: XP_038892146.1 (gibberellin 2-beta-dioxygenase 6-like [Benincasa hispida])

HSP 1 Score: 441 bits (1133), Expect = 6.68e-154
Identity = 207/228 (90.79%), Postives = 220/228 (96.49%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLF VPFEKK TSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKV+E ACYG+FIYLR
Sbjct: 101 MKLFRVPFEKKSTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVAEPACYGEFIYLR 160

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+ASAMSK+AR LAGVLV+S+G RKEL +DICDESTCFLRLNHYPICPFSGEVSGL
Sbjct: 161 EVMEELASAMSKLARCLAGVLVQSLGHRKELFDDICDESTCFLRLNHYPICPFSGEVSGL 220

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLT+LHQD GGGLQ+MKGSQW+AVKPNP+ALVVNIGDLLQAWSNDTYKSAKHR
Sbjct: 221 VPHTDSDFLTVLHQDCGGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSAKHR 280

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVANP RERYSIAYFLCPSYHSQIGSCR+PSPYRHFTFAEYRKQVRDD
Sbjct: 281 VVANPKRERYSIAYFLCPSYHSQIGSCRQPSPYRHFTFAEYRKQVRDD 328

[Close]

BLAST of Cucsat.G4365 vs. NCBI nr
Match: KAG6600809.1 (Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 427 bits (1099), Expect = 1.15e-148
Identity = 201/228 (88.16%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLFGVPFEKK TSGILD+SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FI LR
Sbjct: 94  MKLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLR 153

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVE +G RKE+ +DIC ESTCFLRLNHYPICPF G+VSGL
Sbjct: 154 EVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICHESTCFLRLNHYPICPFPGDVSGL 213

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQD  GGLQ+MKGSQW+AVKPNP+ALVVNIGDLLQAWSNDTYKS KHR
Sbjct: 214 VPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHR 273

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVAN MRERYSIAYFLCPSYHS IGSCR+PSPYRHFTFAEYRKQVRDD
Sbjct: 274 VVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDD 321

[Close]

BLAST of Cucsat.G4365 vs. NCBI nr
Match: KAG7031445.1 (Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 427 bits (1097), Expect = 1.52e-148
Identity = 201/227 (88.55%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 2   KLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLRE 61
           KLFGVPFEKK TSGILD+SYRWGTPTATHPNQFSWSEAFHI LTKVSEAACYG+FI LRE
Sbjct: 95  KLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFINLRE 154

Query: 62  VMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLV 121
           VMEE+A+AMSK+AR LAGVLVE +G RKE+ +DICDESTCFLRLNHYPICPF G+VSGLV
Sbjct: 155 VMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLV 214

Query: 122 PHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRV 181
           PHTDSDFLTILHQD  GGLQ+MKGSQW+AVKPNP+ALVVNIGDLLQAWSNDTYKS KHRV
Sbjct: 215 PHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRV 274

Query: 182 VANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VAN MRERYSIAYFLCPSYHS IGSCR+PSPYRHFTFAEYRKQVRDD
Sbjct: 275 VANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDD 321

[Close]

BLAST of Cucsat.G4365 vs. NCBI nr
Match: XP_022989785.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita maxima])

HSP 1 Score: 428 bits (1101), Expect = 2.29e-148
Identity = 201/228 (88.16%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLFGVPFEKK TSGILD+SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FI LR
Sbjct: 134 MKLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLR 193

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVE +G RKE+ +DICDESTCFLRLNHYPICPF G+VSGL
Sbjct: 194 EVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGL 253

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQD  GGLQ+MKGSQW+AV PNP+ALVVNIGDLLQAWSNDTYKS KHR
Sbjct: 254 VPHTDSDFLTILHQDRSGGLQLMKGSQWVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHR 313

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVAN MRERYSIAYFLCPSYHS IGSCR+PSPYRHFTFAEYRKQVRDD
Sbjct: 314 VVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDD 361

[Close]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL
Match: A0A0A0KIW0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 474 bits (1220), Expect = 1.90e-167
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR
Sbjct: 102 IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 161

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL
Sbjct: 162 EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 221

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR
Sbjct: 222 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 281

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD
Sbjct: 282 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 329

[Close]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL
Match: A0A6J1JNB9 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111486865 PE=3 SV=1)

HSP 1 Score: 428 bits (1101), Expect = 1.11e-148
Identity = 201/228 (88.16%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLFGVPFEKK TSGILD+SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FI LR
Sbjct: 134 MKLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLR 193

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVE +G RKE+ +DICDESTCFLRLNHYPICPF G+VSGL
Sbjct: 194 EVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGL 253

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQD  GGLQ+MKGSQW+AV PNP+ALVVNIGDLLQAWSNDTYKS KHR
Sbjct: 254 VPHTDSDFLTILHQDRSGGLQLMKGSQWVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHR 313

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVAN MRERYSIAYFLCPSYHS IGSCR+PSPYRHFTFAEYRKQVRDD
Sbjct: 314 VVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDD 361

[Close]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL
Match: A0A6J1FTS8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447241 PE=3 SV=1)

HSP 1 Score: 427 bits (1097), Expect = 4.51e-148
Identity = 200/228 (87.72%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLFGVPFEKK TSGILD+SYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYG+FI LR
Sbjct: 134 MKLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLR 193

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVE +G RKE+ +DICDESTCFLRLNHYPICPF G+VSGL
Sbjct: 194 EVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGL 253

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSDFLTILHQD  GGLQ+MKGSQW+AVKPNP+ALVVNIGDLLQAWSNDTYKS KH 
Sbjct: 254 VPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHM 313

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVAN MRERYSIAYFLCPSYHS IGSC++PSPYRHFTFAEYRKQVRDD
Sbjct: 314 VVANSMRERYSIAYFLCPSYHSPIGSCKQPSPYRHFTFAEYRKQVRDD 361

[Close]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL
Match: A0A6J1JB12 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483364 PE=3 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 3.41e-142
Identity = 194/228 (85.09%), Postives = 210/228 (92.11%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLF VPFEKK TSGILDNSYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYG+FI+LR
Sbjct: 101 MKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLR 160

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVES+G RKE+ ED+CDESTCFLRLNHYPICPF G+V+GL
Sbjct: 161 EVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGL 220

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSD+LTILHQDSGGGLQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHR
Sbjct: 221 VPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHR 280

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VVAN  RERYS AYFLCPS HS IGSCR+PSPYRHFTF EYRKQVRDD
Sbjct: 281 VVANSARERYSTAYFLCPSDHSGIGSCRQPSPYRHFTFGEYRKQVRDD 328

[Close]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL
Match: A0A6J1FVR3 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447734 PE=3 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 3.41e-142
Identity = 194/228 (85.09%), Postives = 209/228 (91.67%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLR 60
           +KLF VPFEKK TSGILDNSYRWG  TAT PNQFSWSEAFHIPLTKVSEA CYGDFI+LR
Sbjct: 101 MKLFAVPFEKKSTSGILDNSYRWGAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLR 160

Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           EVMEE+A+AMSK+AR LAGVLVES+G RKE+ ED+CDESTCFLRLNHYPICPF GEV+GL
Sbjct: 161 EVMEELATAMSKLARSLAGVLVESLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGL 220

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
           VPHTDSD+LTILHQDSGGGLQ+MKGSQW+AVKP P+ALVVNIGDLLQAWSNDTYKS KHR
Sbjct: 221 VPHTDSDYLTILHQDSGGGLQLMKGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHR 280

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 228
           VV N +RERYS AYFLCPS  S IGSCR+PSPYRHFTF EYRKQVRDD
Sbjct: 281 VVTNSVRERYSTAYFLCPSNQSGIGSCRQPSPYRHFTFGEYRKQVRDD 328

[Close]

BLAST of Cucsat.G4365 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 253.8 bits (647), Expect = 1.3e-67
Identity = 121/229 (52.84%), Postives = 158/229 (69.00%), Query Frame = 0


Query: 1   IKLFGVPFEKKFTSGILD-NSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYL 60
           I++F  PF+KK  S      SYRWGTP+AT   Q SWSEAFH+P+T +S+     DF  L
Sbjct: 95  IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFTTL 154

Query: 61  REVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSG 120
              ME+ AS    +A  LA VL E  GQ     ++ C  +TC+LR+N YP CP   EV G
Sbjct: 155 SSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYG 214

Query: 121 LVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKH 180
           L+PHTDSDFLTIL+QD  GGLQ++K ++W+AVKPNP+AL++NIGDL QAWSN  YKS +H
Sbjct: 215 LMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEH 274

Query: 181 RVVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 229
           RV+ NP  ER+S AYF+CPSY + I    +   YR+F+F E+R+QV++D
Sbjct: 275 RVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQED 320

[Close]

BLAST of Cucsat.G4365 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-49
Identity = 103/230 (44.78%), Postives = 139/230 (60.43%), Query Frame = 0


Query: 2   KLFGVPFEKKFT---SGILDNSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIY 61
           KLF  PF  K     S +  NSYRWG P+AT P Q+S SEAFHI L++VS  +   D   
Sbjct: 94  KLFDQPFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNN 153

Query: 62  LREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVS 121
           LR ++E     +++VA+ +  +L + +    E  E+I +    FLRLN Y    F  EV 
Sbjct: 154 LRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVF 213

Query: 122 GLVPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAK 181
           GLVPHTD+ FLTIL QD  GGL++    QW++VKP  +AL VNIGD+ QA SN  Y+S +
Sbjct: 214 GLVPHTDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVR 273

Query: 182 HRVVANPMRERYSIAYFLCPSYHSQIGSCREPSPYRHFTFAEYRKQVRDD 229
           HRV++    ER SIA+F+CP   ++I     P  YR F+F EY++Q   D
Sbjct: 274 HRVISPANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHD 321

[Close]

BLAST of Cucsat.G4365 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.3e-30
Identity = 69/172 (40.12%), Postives = 100/172 (58.14%), Query Frame = 0


Query: 58  YLREVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEV 117
           + REV +E A  + K+A +L  ++  S+G   + L    +E T FLR NHYP CP     
Sbjct: 157 HFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELA 216

Query: 118 SGLVPHTDSDFLTILHQDSGGGLQVMKGS--QWLAVKPNPQALVVNIGDLLQAWSNDTYK 177
            G+  H D   LT+L QDS GGLQV + S  QW+ VKP   AL++N+G+ +Q W+ND Y 
Sbjct: 217 LGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYW 276

Query: 178 SAKHRVVANPMRERYSIAYFLCPSYHSQIGSCRE------PSPYRHFTFAEY 222
           SA+HRVV N  +ER+SI +F  PS+ + I    E      P  Y+ + + ++
Sbjct: 277 SAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 328

[Close]

BLAST of Cucsat.G4365 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-29
Identity = 64/149 (42.95%), Postives = 87/149 (58.39%), Query Frame = 0


Query: 61  EVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGL 120
           +V +E   AMS ++ K+  +L  S+G +++   +  +E+   +RLN+YP C       G 
Sbjct: 185 KVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGT 244

Query: 121 VPHTDSDFLTILHQDSGGGLQVMKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHR 180
            PH D   LTILHQD   GLQV   +QW +++PNP+A VVNIGD   A SND YKS  HR
Sbjct: 245 GPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHR 304

Query: 181 VVANPMRERYSIAYFLCPSYHSQIGSCRE 210
            V N   ER S+A+FLCP     +   RE
Sbjct: 305 AVVNSESERKSLAFFLCPKKDRVVTPPRE 333

[Close]

BLAST of Cucsat.G4365 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.4e-29
Identity = 67/170 (39.41%), Postives = 95/170 (55.88%), Query Frame = 0


Query: 60  REVMEEVASAMSKVARKLAGVLVESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSG 119
           RE  E  A    K+A KL  ++  S+G  KE   D   E   F R+N YP CP      G
Sbjct: 154 REACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALG 213

Query: 120 LVPHTDSDFLTILHQDSGGGLQVMKGSQ--WLAVKPNPQALVVNIGDLLQAWSNDTYKSA 179
           +  H D+D +++L QD  GGLQV + S   W  ++P P ALV+NIG+ ++ W+ND Y SA
Sbjct: 214 VGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSA 273

Query: 180 KHRVVANPMRERYSIAYFLCPSYHSQIGSCRE------PSPYRHFTFAEY 222
           +HRVV N  RERYSI +FL PS+  ++    E      P  Y+ + + ++
Sbjct: 274 EHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGYKWGKF 323

The following BLAST results are available for this feature:

BLAST of Cucsat.G4365 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XP65	6.6e-77	58.72	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
O49561	1.8e-66	52.84	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q8LGZ9	1.1e-58	49.79	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	2.9e-48	44.78	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q39110	1.5e-28	42.95	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]

BLAST of Cucsat.G4365 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN49660.1	3.92e-167	100.00	hypothetical protein Csa_018434 [Cucumis sativus]	[more]
XP_038892146.1	6.68e-154	90.79	gibberellin 2-beta-dioxygenase 6-like [Benincasa hispida]	[more]
KAG6600809.1	1.15e-148	88.16	Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia...	[more]
KAG7031445.1	1.52e-148	88.55	Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022989785.1	2.29e-148	88.16	gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita maxima]	[more]

BLAST of Cucsat.G4365 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIW0	1.90e-167	100.00	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1JNB9	1.11e-148	88.16	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148686...	[more]
A0A6J1FTS8	4.51e-148	87.72	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A6J1JB12	3.41e-142	85.09	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148336...	[more]
A0A6J1FVR3	3.41e-142	85.09	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]

BLAST of Cucsat.G4365 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	1.3e-67	52.84	gibberellin 2-oxidase 8	[more]
AT1G50960.1	2.1e-49	44.78	gibberellin 2-oxidase 7	[more]
AT3G19000.1	1.3e-30	40.12	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25420.1	1.1e-29	42.95	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	1.4e-29	39.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..138 e-value: 1.1E-33 score: 118.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 66..131 e-value: 5.6E-15 score: 55.7
None	No IPR available	PANTHER	PTHR47990:SF24	OS02G0630300 PROTEIN	coord: 1..135
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 1..135
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 22..131
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 61..148 score: 8.843623

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4365.T2	Cucsat.G4365.T2	mRNA
Cucsat.G4365.T1	Cucsat.G4365.T1	mRNA
Cucsat.G4365.T3	Cucsat.G4365.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding