Cucsat.G4250 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4250
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	NAC domain-containing protein
Location	ctg1170: 82013 .. 84056 (-)
RNA-Seq Expression	Cucsat.G4250
Synteny	Cucsat.G4250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAACAATACAAATTTAATAAAATTTGGAAAGGAAAAATGGAGAGATTTGAATAAGAAGAGATGAGAAGAGAAGAAGATATATATATCTCCGGGAGATGTGTGAGGAGGGGATGCTGAGAATCTGCATTGTGCGCTTTAATCCAAAAGAATGTGCGTAAGCCATGACAAAATCACCTGCATCCGTGTCCATCGCTCACGCATGCACTTCACGTCTTAATCTGCCACCTCCTTAAGCCGGCGCTTCTTTTCTCTCGTCGACAAACCAATTGCCCTTATATCTCACGTGCTTTTTCACTCCTTCTCCCCACCTCTCCCCTCCTCCACCTCTATAAATACCTTTCCCTCCCATTGTTTCCACGTTTGAACCGTCGACGATTAGCCCCCAACAAACAATTAACTCAACACACAAATCAATTTTCAGAGAACCAAACCGAGAAATGACAACCGACTTGACTCAGCTTCCCCCCGGTTTCCGCTTTCATCCAACCGACGAGGAGCTTGTTATCCACTATCTCTGCCGGAGATGCGCATCGCAGCCTATTGCCGTTCCCATCATTGCTGAGATCGATCTCTATAAATTCGATCCATGGGACCTTCCTGGTATAATTGAGCTCCAAGAGTTTTTCGATTTCTATTTCTATTTCAATTTCAATTTGTCTTTCTGATCGATCTTCCTTCTTACCCTTCAAATTTCCCCTTACAGAACTGGCCTTACACGGAGAGAAAGAATGGTATTTCTTCTCTCCAAGGGAGCGGAAATATCCAAACGGTTCACGACCCAACCGGGCGGCCGGTTCGGGTTACTGGAAGGCAACCGGAGCGGACAAGCCTATTGGGCATCCTAAACCGGTTGGAATCAAGAAGGCCTTGGTTTTCTACGCCGGAAAAGCTCCGAGAGGAGAGAAAACCAATTGGATTATGCATGAGTACCGGCTCGCCGATGTGGACCGGTCGGCTCGCAACAAGAACAATCTAAAGGTAATTTTAATCGAATAAAAAAAAAAATTCTAGGGCTTTTTTCCCCCTTTTGCAAAATCTAAATCAACGGTCGTAATTTTGCAGCTTGACGATTGGGTTCTGTGCCGGATTTACAACAAGAAAGGTGTAATAGAGAAGCGATATCAGATGGCAATGAATCGAAACACGAAGGGTTTGGAAGAGGTAGAAGAGGAGAAGCCGAAGATTTTCATCAGTGGAGGCGGCGGCGGCGGCGGCGATGTGTTAATGCCGTCAGCAACGGCGTCGGTAGGTGATTTCGTACATTTCGAAACATCGGATTCAATACCGCGATTACACACAGATTCAAGCGGTTCGGAGCAGGTGGTGTCGCCGGAGTTCACGACGGGGGAAGTTGAAAGCGAGCGAAAATGGAAGGAAATGGAAAACGCTTTTAGTTATCGATACAATTACATAGATGACTCATTATTAGACCATGAATTTGCAACGCAGTTTCAGAACGGTAATCAGATGTCGCCGCTGCAGGATATGTTCATGTACCTGCAGAAGCCCTTTTGAACCAAGAACCCTCTGATTCAGATTTTGTTCATCATCGATCCATCACAACAATCCTTCCAACCTCCAAATTTCTAACAGCCGTTGACGGTGTGTGTCATCGAGGGTCGCACGAGACGGGAGTCTGTGAGTGCGCCAGGTGTATAGATTAAAAGAAAATGGAAGTAATAGAATACGGATATGCAGAGATAGAGATGAGAGGAGAGTGTAGTTAATTAGTGTTATTGTAGGAGCCAGGCGTATGTTGTTGGCGTACACTTTTGGATTAGATCGGACGGACCAGATTTAATCAGTGATTGGTTGTGATTATTGATCGGCGCTGTGTGGTATAATTTTATATTTATTACTATTTTGGAAAGATTTAATTAGCCTTCTAATCTTTTATTTGTTTCTTAGCTTCATCTATCATCTATCATCTATCATCTTAGTCTTAGCTTTGTTTATATATTTAAATTTTATCTTATTTATCATCCTTCTTTTTCTGATATGATGATTGATGATGAGAAATTACTTGAAAAATAATATCGAC

Coding sequence (CDS)

ATGCATGAGTACCGGCTCGCCGATGTGGACCGGTCGGCTCGCAACAAGAACAATCTAAAGCTTGACGATTGGGTTCTGTGCCGGATTTACAACAAGAAAGGTGTAATAGAGAAGCGATATCAGATGGCAATGAATCGAAACACGAAGGGTTTGGAAGAGGTAGAAGAGGAGAAGCCGAAGATTTTCATCAGTGGAGGCGGCGGCGGCGGCGGCGATGTGTTAATGCCGTCAGCAACGGCGTCGGTAGGTGATTTCGTACATTTCGAAACATCGGATTCAATACCGCGATTACACACAGATTCAAGCGGTTCGGAGCAGGTGGTGTCGCCGGAGTTCACGACGGGGGAAGTTGAAAGCGAGCGAAAATGGAAGGAAATGGAAAACGCTTTTAGTTATCGATACAATTACATAGATGACTCATTATTAGACCATGAATTTGCAACGCAGTTTCAGAACGGTAATCAGATGTCGCCGCTGCAGGATATGTTCATGTACCTGCAGAAGCCCTTTTGA

Protein sequence

MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPKIFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESERKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF

Homology

[Close]

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot
Match: Q39013 (NAC domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=NAC002 PE=1 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 3.6e-35
Identity = 91/179 (50.84%), Postives = 116/179 (64.80%), Query Frame = 0


Query: 1   MHEYRLADVDRSAR-NKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKP 60
           MHEYRLADVDRS R  KN+L+LDDWVLCRIYNKKG  E+R            +E+ EEKP
Sbjct: 126 MHEYRLADVDRSVRKKKNSLRLDDWVLCRIYNKKGATERR---GPPPPVVYGDEIMEEKP 185

Query: 61  KIFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLH-TDSSGSEQVVSPEFTTGEVE 120
           K+          +++MP       +F +F+TSDS+P+LH TDSS SEQVVSPEFT+ EV+
Sbjct: 186 KV---------TEMVMPPPPQQTSEFAYFDTSDSVPKLHTTDSSCSEQVVSPEFTS-EVQ 245

Query: 121 SERKWKEM-------ENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 171
           SE KWK+         N   + +NYI D+ +D+ F     + NQM PLQDMFMY+QKP+
Sbjct: 246 SEPKWKDWSAVSNDNNNTLDFGFNYI-DATVDNAFGGG-GSSNQMFPLQDMFMYMQKPY 289

[Close]

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot
Match: Q7F2L3 (NAC domain-containing protein 48 OS=Oryza sativa subsp. japonica OX=39947 GN=NAC048 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.2e-20
Identity = 78/182 (42.86%), Postives = 99/182 (54.40%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMA-----MNRNTKGLEEVE 60
           MHEYRLADVDRSAR KN+L+LDDWVLCRIYNKKG +EK    A     M  +  G+    
Sbjct: 128 MHEYRLADVDRSARKKNSLRLDDWVLCRIYNKKGGLEKPPAAAVAAAGMVSSGGGV---- 187

Query: 61  EEKPKIFISGGGGGGGDVLMPSATASVGDFVHF--ETSDSIPRLHTDSSGSEQVVSPEFT 120
           + KP + ++       +     A  +  D   +    SDS+PRLH DSS SEQV+SPEF 
Sbjct: 188 QRKPMVGVNAAVSSPPEQKPVVAGPAFPDLAAYYDRPSDSMPRLHADSSCSEQVLSPEFA 247

Query: 121 TGEVESERKWKEMENAFSYRYNYIDD--SLLDHEFA---TQFQNGNQMSPLQDMFMYLQK 171
             EV+S+ K  E E  F+     I+   S+LD   +        G     LQD+ MY  K
Sbjct: 248 C-EVQSQPKISEWERTFA-TVGPINPAASILDPAGSGGLGGLGGGGSDPLLQDILMYWGK 303

[Close]

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot
Match: Q9CAR0 (NAC transcription factor 32 OS=Arabidopsis thaliana OX=3702 GN=NAC032 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 64/156 (41.03%), Postives = 80/156 (51.28%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRS R KN+L+LDDWVLCRIYNKKGVIEKR     +    GL+ V +  P 
Sbjct: 129 MHEYRLADVDRSVRKKNSLRLDDWVLCRIYNKKGVIEKR----RSDIEDGLKPVTDTCP- 188

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
                                          +S+ RL    SGSEQ VSPEFT       
Sbjct: 189 ------------------------------PESVARL---ISGSEQAVSPEFTCSN---- 238

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQM 157
                + NA  + +NY+ D++ D+E  ++   GNQM
Sbjct: 249 ---GRLSNALDFPFNYV-DAIADNEIVSRLLGGNQM 238

[Close]

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot
Match: Q53NF7 (NAC domain-containing protein 71 OS=Oryza sativa subsp. japonica OX=39947 GN=NAC071 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.0e-17
Identity = 83/208 (39.90%), Postives = 100/208 (48.08%), Query Frame = 0


Query: 1   MHEYRLADVDRSA-------RNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEE 60
           MHEYRLADVDRSA        + N L+LDDWVLCRIYNKKGVIE RY           E+
Sbjct: 128 MHEYRLADVDRSAAARKLSKSSHNALRLDDWVLCRIYNKKGVIE-RYD-----TVDAGED 187

Query: 61  VEEEKPKIFISGG--GGGGGDVLMPSATASVG--------DFVHFETS-----DSIPRLH 120
           V+         GG  GGGGG   M    +  G        + + F+ S     DS+PRLH
Sbjct: 188 VKPAAAAAAAKGGRIGGGGGAAAMKVELSDYGFYDQEPESEMLCFDRSGSADRDSMPRLH 247

Query: 121 TDSSGSEQVVSP-----EFTTG----EVESERK-----WKEMENAFSYRYNY-IDDSLLD 171
           TDSSGSE V+SP     +F  G      ES+       W  ++ A      + ID SL +
Sbjct: 248 TDSSGSEHVLSPSPSPDDFPGGGDHDYAESQPSGGCGGWPGVDWAAVGDDGFVIDSSLFE 307

[Close]

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot
Match: Q9C598 (Protein ATAF2 OS=Arabidopsis thaliana OX=3702 GN=NAC081 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-14
Identity = 59/136 (43.38%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 1   MHEYRLADVDRSA--RNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEK 60
           MHEYRLA+VDRSA    KNNL+LDDWVLCRIYNKKG +EK +               +EK
Sbjct: 126 MHEYRLANVDRSASVNKKNNLRLDDWVLCRIYNKKGTMEKYFP-------------ADEK 185

Query: 61  PKIFISGGGGGGGDVLMPSATASVGDFVHFETSDS-IPRLHTDSSGSE----QVVSPEFT 120
           P+                + T +      F+TSDS  P L  D S S      VVSP+  
Sbjct: 186 PR----------------TTTMAEQSSSPFDTSDSTYPTLQEDDSSSSGGHGHVVSPDVL 230

Query: 121 TGEVESERKWKEMENA 130
             EV+SE KW E+E+A
Sbjct: 246 --EVQSEPKWGELEDA 230

[Close]

BLAST of Cucsat.G4250 vs. NCBI nr
Match: XP_004149802.1 (NAC domain-containing protein 2 [Cucumis sativus] >KGN47707.1 hypothetical protein Csa_018320 [Cucumis sativus])

HSP 1 Score: 344 bits (882), Expect = 1.96e-117
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IFISGGG GGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE
Sbjct: 187 IFISGGGDGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF
Sbjct: 247 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 296

[Close]

BLAST of Cucsat.G4250 vs. NCBI nr
Match: XP_008466736.1 (PREDICTED: NAC domain-containing protein 2 [Cucumis melo] >KAA0055455.1 NAC domain-containing protein 2 [Cucumis melo var. makuwa] >TYK08938.1 NAC domain-containing protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 301 bits (770), Expect = 1.99e-100
Identity = 150/170 (88.24%), Postives = 158/170 (92.94%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKR QMA++RNTKGLE+VEEEKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRCQMAISRNTKGLEDVEEEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IF SGGGG   DVLM S TASVGD+VHFETSDSIPRLHTDSSGSEQVVSPE TTGEVESE
Sbjct: 187 IFTSGGGGA--DVLMASVTASVGDYVHFETSDSIPRLHTDSSGSEQVVSPELTTGEVESE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           RKWKEMENAF+  YNYID+S L+HEFATQF +GNQMSPLQDMFMYLQKPF
Sbjct: 247 RKWKEMENAFNSGYNYIDESSLEHEFATQFHSGNQMSPLQDMFMYLQKPF 294

[Close]

BLAST of Cucsat.G4250 vs. NCBI nr
Match: AHJ38169.1 (NAC domain transcription factor 2 [Citrullus colocynthis])

HSP 1 Score: 266 bits (680), Expect = 9.99e-87
Identity = 133/173 (76.88%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKR Q+A+NRNT   EE E+EKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRNQIAVNRNTNVYEEEEDEKPK 186

Query: 61  IFISGGGGGGGDVLMPS---ATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEV 120
           IF S GG    D L+P+   A ASV D++H++TSDSIPRLHTDSS SE VVSPEFTTGEV
Sbjct: 187 IFTSRGGA---DTLIPAVAAAPASVDDYIHYDTSDSIPRLHTDSSCSEHVVSPEFTTGEV 246

Query: 121 ESERKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           +SE KWKE+ENAF+++YNYID++ LDHEFATQFQ+GNQMSPLQDMFM+L KPF
Sbjct: 247 QSEPKWKELENAFNFQYNYIDNNSLDHEFATQFQSGNQMSPLQDMFMHLPKPF 296

[Close]

BLAST of Cucsat.G4250 vs. NCBI nr
Match: XP_038886712.1 (NAC domain-containing protein 2 [Benincasa hispida])

HSP 1 Score: 265 bits (678), Expect = 1.95e-86
Identity = 131/170 (77.06%), Postives = 149/170 (87.65%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKN+LKLDDWVLCRIYNKKGVIEKR+Q+++ RNT   E+ E+EKPK
Sbjct: 127 MHEYRLADVDRSARNKNDLKLDDWVLCRIYNKKGVIEKRHQISITRNTNVFEDEEDEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IF S GGG      + +A A+V D++HF+TSDSIPRLHTDSS SE VVSPEFTTGEV+SE
Sbjct: 187 IFTSRGGGDSSMPPVTAAPAAVDDYIHFDTSDSIPRLHTDSSCSEHVVSPEFTTGEVQSE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
            KWKE+ENAF++RYNYID SL DHEFATQFQ+GNQMSPLQDMFMYLQKPF
Sbjct: 247 PKWKELENAFNFRYNYIDTSL-DHEFATQFQSGNQMSPLQDMFMYLQKPF 295

[Close]

BLAST of Cucsat.G4250 vs. NCBI nr
Match: KAG7016476.1 (NAC domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249 bits (637), Expect = 4.97e-82
Identity = 129/173 (74.57%), Postives = 148/173 (85.55%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLA VDRSARNK++L+LDDWVLCRIYNKKGVIEKR+ +A+N+NT G E+ E++KP 
Sbjct: 1   MHEYRLAGVDRSARNKSSLRLDDWVLCRIYNKKGVIEKRHPIAVNQNTDGFED-EDKKPT 60

Query: 61  IFISGGGGGGGDVLMPSAT---ASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEV 120
           I  +  GG   D  MP+AT   ASV D++HFETSDSIPRL+TDSS SE VVSPEFTT EV
Sbjct: 61  ILANQYGG---DAFMPAATEAAASVSDYIHFETSDSIPRLNTDSSCSEHVVSPEFTTSEV 120

Query: 121 ESERKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           +SE KWKEMENAF++RYNYID SL DHEFATQFQ+GNQMSPLQDMFMYLQKPF
Sbjct: 121 QSEPKWKEMENAFNFRYNYIDTSL-DHEFATQFQSGNQMSPLQDMFMYLQKPF 168

[Close]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL
Match: A0A0A0KH31 (NAC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382950 PE=4 SV=1)

HSP 1 Score: 344 bits (882), Expect = 9.49e-118
Identity = 169/170 (99.41%), Postives = 169/170 (99.41%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IFISGGG GGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE
Sbjct: 187 IFISGGGDGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF
Sbjct: 247 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 296

[Close]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL
Match: A0A5A7UP93 (NAC domain-containing protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00270 PE=4 SV=1)

HSP 1 Score: 301 bits (770), Expect = 9.63e-101
Identity = 150/170 (88.24%), Postives = 158/170 (92.94%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKR QMA++RNTKGLE+VEEEKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRCQMAISRNTKGLEDVEEEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IF SGGGG   DVLM S TASVGD+VHFETSDSIPRLHTDSSGSEQVVSPE TTGEVESE
Sbjct: 187 IFTSGGGGA--DVLMASVTASVGDYVHFETSDSIPRLHTDSSGSEQVVSPELTTGEVESE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           RKWKEMENAF+  YNYID+S L+HEFATQF +GNQMSPLQDMFMYLQKPF
Sbjct: 247 RKWKEMENAFNSGYNYIDESSLEHEFATQFHSGNQMSPLQDMFMYLQKPF 294

[Close]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL
Match: A0A1S3CRZ3 (NAC domain-containing protein 2 OS=Cucumis melo OX=3656 GN=LOC103504077 PE=4 SV=1)

HSP 1 Score: 301 bits (770), Expect = 9.63e-101
Identity = 150/170 (88.24%), Postives = 158/170 (92.94%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKR QMA++RNTKGLE+VEEEKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRCQMAISRNTKGLEDVEEEKPK 186

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
           IF SGGGG   DVLM S TASVGD+VHFETSDSIPRLHTDSSGSEQVVSPE TTGEVESE
Sbjct: 187 IFTSGGGGA--DVLMASVTASVGDYVHFETSDSIPRLHTDSSGSEQVVSPELTTGEVESE 246

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           RKWKEMENAF+  YNYID+S L+HEFATQF +GNQMSPLQDMFMYLQKPF
Sbjct: 247 RKWKEMENAFNSGYNYIDESSLEHEFATQFHSGNQMSPLQDMFMYLQKPF 294

[Close]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL
Match: W6GE41 (NAC domain transcription factor 2 OS=Citrullus colocynthis OX=252529 GN=NAC2 PE=2 SV=1)

HSP 1 Score: 266 bits (680), Expect = 4.83e-87
Identity = 133/173 (76.88%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKR Q+A+NRNT   EE E+EKPK
Sbjct: 127 MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRNQIAVNRNTNVYEEEEDEKPK 186

Query: 61  IFISGGGGGGGDVLMPS---ATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEV 120
           IF S GG    D L+P+   A ASV D++H++TSDSIPRLHTDSS SE VVSPEFTTGEV
Sbjct: 187 IFTSRGGA---DTLIPAVAAAPASVDDYIHYDTSDSIPRLHTDSSCSEHVVSPEFTTGEV 246

Query: 121 ESERKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           +SE KWKE+ENAF+++YNYID++ LDHEFATQFQ+GNQMSPLQDMFM+L KPF
Sbjct: 247 QSEPKWKELENAFNFQYNYIDNNSLDHEFATQFQSGNQMSPLQDMFMHLPKPF 296

[Close]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL
Match: A0A6J1JUT5 (NAC domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487693 PE=4 SV=1)

HSP 1 Score: 249 bits (635), Expect = 3.25e-80
Identity = 127/171 (74.27%), Postives = 140/171 (81.87%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGL-EEVEEEKP 60
           MHEYRLADVDRSARNKN+L+LDDWVLCRIYNKKGVI+KR   A+NRNT G  EEVE+EKP
Sbjct: 127 MHEYRLADVDRSARNKNSLRLDDWVLCRIYNKKGVIDKRNHAAVNRNTNGFKEEVEDEKP 186

Query: 61  KIFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVES 120
            IF + GG GG    + +  ASV D +HFETSDSIPRLH DSS SE VVSPEF T EV+S
Sbjct: 187 NIFTNHGGAGGFMPAVMAVPASVDDCIHFETSDSIPRLHADSSCSEHVVSPEFATSEVQS 246

Query: 121 ERKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 170
           E KWKE +NAF +RYNYID+SL D EFAT FQ GNQMSPLQDMFMYLQKPF
Sbjct: 247 EPKWKEFQNAFDFRYNYIDNSL-DQEFATPFQGGNQMSPLQDMFMYLQKPF 296

[Close]

BLAST of Cucsat.G4250 vs. TAIR 10
Match: AT1G01720.1 (NAC (No Apical Meristem) domain transcriptional regulator superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 2.5e-36
Identity = 91/179 (50.84%), Postives = 116/179 (64.80%), Query Frame = 0


Query: 1   MHEYRLADVDRSAR-NKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKP 60
           MHEYRLADVDRS R  KN+L+LDDWVLCRIYNKKG  E+R            +E+ EEKP
Sbjct: 126 MHEYRLADVDRSVRKKKNSLRLDDWVLCRIYNKKGATERR---GPPPPVVYGDEIMEEKP 185

Query: 61  KIFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLH-TDSSGSEQVVSPEFTTGEVE 120
           K+          +++MP       +F +F+TSDS+P+LH TDSS SEQVVSPEFT+ EV+
Sbjct: 186 KV---------TEMVMPPPPQQTSEFAYFDTSDSVPKLHTTDSSCSEQVVSPEFTS-EVQ 245

Query: 121 SERKWKEM-------ENAFSYRYNYIDDSLLDHEFATQFQNGNQMSPLQDMFMYLQKPF 171
           SE KWK+         N   + +NYI D+ +D+ F     + NQM PLQDMFMY+QKP+
Sbjct: 246 SEPKWKDWSAVSNDNNNTLDFGFNYI-DATVDNAFGGG-GSSNQMFPLQDMFMYMQKPY 289

[Close]

BLAST of Cucsat.G4250 vs. TAIR 10
Match: AT1G77450.1 (NAC domain containing protein 32 )

HSP 1 Score: 94.0 bits (232), Expect = 1.3e-19
Identity = 64/156 (41.03%), Postives = 80/156 (51.28%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEKPK 60
           MHEYRLADVDRS R KN+L+LDDWVLCRIYNKKGVIEKR     +    GL+ V +  P 
Sbjct: 129 MHEYRLADVDRSVRKKNSLRLDDWVLCRIYNKKGVIEKR----RSDIEDGLKPVTDTCP- 188

Query: 61  IFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTGEVESE 120
                                          +S+ RL    SGSEQ VSPEFT       
Sbjct: 189 ------------------------------PESVARL---ISGSEQAVSPEFTCSN---- 238

Query: 121 RKWKEMENAFSYRYNYIDDSLLDHEFATQFQNGNQM 157
                + NA  + +NY+ D++ D+E  ++   GNQM
Sbjct: 249 ---GRLSNALDFPFNYV-DAIADNEIVSRLLGGNQM 238

[Close]

BLAST of Cucsat.G4250 vs. TAIR 10
Match: AT5G08790.1 (NAC (No Apical Meristem) domain transcriptional regulator superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 8.5e-16
Identity = 59/136 (43.38%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 1   MHEYRLADVDRSA--RNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEK 60
           MHEYRLA+VDRSA    KNNL+LDDWVLCRIYNKKG +EK +               +EK
Sbjct: 126 MHEYRLANVDRSASVNKKNNLRLDDWVLCRIYNKKGTMEKYFP-------------ADEK 185

Query: 61  PKIFISGGGGGGGDVLMPSATASVGDFVHFETSDS-IPRLHTDSSGSE----QVVSPEFT 120
           P+                + T +      F+TSDS  P L  D S S      VVSP+  
Sbjct: 186 PR----------------TTTMAEQSSSPFDTSDSTYPTLQEDDSSSSGGHGHVVSPDVL 230

Query: 121 TGEVESERKWKEMENA 130
             EV+SE KW E+E+A
Sbjct: 246 --EVQSEPKWGELEDA 230

[Close]

BLAST of Cucsat.G4250 vs. TAIR 10
Match: AT5G63790.1 (NAC domain containing protein 102 )

HSP 1 Score: 72.8 bits (177), Expect = 3.0e-13
Identity = 65/185 (35.14%), Postives = 80/185 (43.24%), Query Frame = 0


Query: 1   MHEYRLADVDRSA--RNKNNLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEVEEEK 60
           MHEYRLA+VDRSA    KNNL+LDDWVLCRIYNKKG +EK    A  + T+ +       
Sbjct: 169 MHEYRLANVDRSASTNKKNNLRLDDWVLCRIYNKKGTMEKYLPAAAEKPTEKMST----- 228

Query: 61  PKIFISGGGGGGGDVLMPSATASVGDFVHFETSDSIPRLHTDSSGSEQVVSPEFTTG--- 120
                                                   +DS  S  V+SP+ T     
Sbjct: 229 ----------------------------------------SDSRCSSHVISPDVTCSDNW 288

Query: 121 EVESERKWKEMENAFSYRYNYIDDS--------LLDHEFATQFQNGNQMSPLQDMFM--Y 171
           EVESE KW  +E+A      + DD+        L +  F  QFQ   Q S   D F   +
Sbjct: 289 EVESEPKWINLEDALE---AFNDDTSMFSSIGLLQNDAFVPQFQ--YQSSDFVDSFQDPF 303

[Close]

BLAST of Cucsat.G4250 vs. TAIR 10
Match: AT1G69490.1 (NAC-like, activated by AP3/PI )

HSP 1 Score: 46.2 bits (108), Expect = 3.0e-05
Identity = 24/66 (36.36%), Postives = 41/66 (62.12%), Query Frame = 0


Query: 1   MHEYRLADVDRSARNKN-NLKLDDWVLCRIYNKKGVIEKRYQMAMNRNTKGLEEV-EEEK 60
           MHEYRL D  +++  +N +++LD+WVLCRIY K+G         +N     ++EV  E++
Sbjct: 129 MHEYRLHDSRKASTKRNGSMRLDEWVLCRIYKKRGA-----SKLLNEQEGFMDEVLMEDE 188

Query: 61  PKIFIS 65
            K+ ++
Sbjct: 189 TKVVVN 189

The following BLAST results are available for this feature:

BLAST of Cucsat.G4250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39013	3.6e-35	50.84	NAC domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=NAC002 PE=1 S...	[more]
Q7F2L3	3.2e-20	42.86	NAC domain-containing protein 48 OS=Oryza sativa subsp. japonica OX=39947 GN=NAC...	[more]
Q9CAR0	1.8e-18	41.03	NAC transcription factor 32 OS=Arabidopsis thaliana OX=3702 GN=NAC032 PE=2 SV=1	[more]
Q53NF7	2.0e-17	39.90	NAC domain-containing protein 71 OS=Oryza sativa subsp. japonica OX=39947 GN=NAC...	[more]
Q9C598	1.2e-14	43.38	Protein ATAF2 OS=Arabidopsis thaliana OX=3702 GN=NAC081 PE=1 SV=1	[more]

BLAST of Cucsat.G4250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149802.1	1.96e-117	99.41	NAC domain-containing protein 2 [Cucumis sativus] >KGN47707.1 hypothetical prote...	[more]
XP_008466736.1	1.99e-100	88.24	PREDICTED: NAC domain-containing protein 2 [Cucumis melo] >KAA0055455.1 NAC doma...	[more]
AHJ38169.1	9.99e-87	76.88	NAC domain transcription factor 2 [Citrullus colocynthis]	[more]
XP_038886712.1	1.95e-86	77.06	NAC domain-containing protein 2 [Benincasa hispida]	[more]
KAG7016476.1	4.97e-82	74.57	NAC domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of Cucsat.G4250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KH31	9.49e-118	99.41	NAC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382950 PE=4 SV...	[more]
A0A5A7UP93	9.63e-101	88.24	NAC domain-containing protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3CRZ3	9.63e-101	88.24	NAC domain-containing protein 2 OS=Cucumis melo OX=3656 GN=LOC103504077 PE=4 SV=...	[more]
W6GE41	4.83e-87	76.88	NAC domain transcription factor 2 OS=Citrullus colocynthis OX=252529 GN=NAC2 PE=...	[more]
A0A6J1JUT5	3.25e-80	74.27	NAC domain-containing protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487693...	[more]

BLAST of Cucsat.G4250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01720.1	2.5e-36	50.84	NAC (No Apical Meristem) domain transcriptional regulator superfamily protein	[more]
AT1G77450.1	1.3e-19	41.03	NAC domain containing protein 32	[more]
AT5G08790.1	8.5e-16	43.38	NAC (No Apical Meristem) domain transcriptional regulator superfamily protein	[more]
AT5G63790.1	3.0e-13	35.14	NAC domain containing protein 102	[more]
AT1G69490.1	3.0e-05	36.36	NAC-like, activated by AP3/PI	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036093	NAC domain superfamily	GENE3D	2.170.150.80	NAC domain	coord: 17..162 e-value: 3.4E-60 score: 204.3
IPR036093	NAC domain superfamily	SUPERFAMILY	101941	NAC domain	coord: 4..158
IPR003441	NAC domain	PFAM	PF02365	NAM	coord: 9..132 e-value: 9.1E-38 score: 130.1
IPR003441	NAC domain	PROSITE	PS51005	NAC	coord: 8..158 score: 60.700958
None	No IPR available	PANTHER	PTHR31719:SF174	NAC DOMAIN-CONTAINING PROTEIN 2-LIKE	coord: 1..275
None	No IPR available	PANTHER	PTHR31719	NAC TRANSCRIPTION FACTOR 56	coord: 1..275

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4250.T2	Cucsat.G4250.T2	mRNA
Cucsat.G4250.T1	Cucsat.G4250.T1	mRNA
Cucsat.G4250.T3	Cucsat.G4250.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding