Cucsat.G4163 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G4163
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	eukaryotic translation initiation factor 5-like
Location	ctg1055: 88933 .. 91965 (-)
RNA-Seq Expression	Cucsat.G4163
Synteny	Cucsat.G4163

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCCCTAATTGGTGACGTGTAGGGTTTTCTTTCTACCATTCCTTCGAGCGTGTATATAAGCCCTTTCCTTCTCCCCATCAAAATCCCCATCGGCTTCTTCGTCACCGCGAAAACAAGAGCCAAAGCCTTTAAGGATCAGTTTTTTTTTCCTCCTATCACGACCATTCATCACTGTAAGGCAAGAATTTTTCTTTGTTTTTCTTGTTTGATCTAACTTCATCACCGTTTCTTGTTGATTTATCGTACTTGATTTGTTGTTTTTGTTATTGTTTGAATCGATTATAAGTTACTGATGATTGTTTTGTCTCTAACGTCTTTGATTTAATGAAATCTTCACGATCGGTATTCCGATTATAGATTTTTTATGGATTTTTGTGAGTTTCCTGCCGTATTGTTCAGATCTGTTGATTTACTTCTTTGTTTGGTATGTTTTTTCTAAATTGCATCTTTGATCGGAATGAAAAAATTGTAATCGGCGTTGTAGATTGGTGTTTGTTTATGTAAAGCTGATGAGAGCGATTATTGAATCTTATCTGTGAATTCGATTTTTATTATTCTTTCTATCTAGGATTTCATTTAGGTTTGGGATATTCGTTGTATCCGTTTGTGTTTAATTGTGTATTGCATTTCGTATCTCTTCATCACTTAAAATTTTCTCGATTTTTTATTTTCTAGTTGTAAATGATAGAGTCTGCGTTTATCTAGTAAGATGTGTGTTAAATCAAGAATGGAGCTTCAAGCTAGAATGTCTTTCATTTTGGGTATGACTGTAAGATCATTTTCGTCTTCTAGAACTTGCTCGATTTGATTTGAACTGCCGGTCAATGTTTTCACTGGTTTTAGCTGGAATTTAGAATTAGTCAATTGAGATTACGAATAACATATTTGTGCGCGATCATTTGCACTTGAATGATGGTATTTATGTTGACCGTGAACGTGTTTTAAGTTCTGATTATTCATTCTGAAGTAATGTTTGTTCTAGATCTTTATTTCCCAATGGTTTTTTTTTTTCTATTTTCTTGTAATATGGTTCTGTTCAACCTTAGTCATAAGGTAATCTTGCGTATTATTTGTTCTTAATTCTCAGGAAGTTGGCCATTTGGTCTGCTGTTCTGTAGTTCAAAGTGGAGGGCGTTACGTTTGAAACTCCTTTTATGTCTGAACGGGCCTCTCTCTTTTTGTCATCGATTGGATTGCTTGGAGAGTATTGTCCTACGTCTAGGAAAAAGATCAGCTGTGCTGAGACCATTGGCTGCATGCATCTTGAGTTGCTAGGAGCTCATAGAAAAGCACGAGGTCTTGGTTTCCATTTTATGCGGTCTTTCCCAGCTGCTCAAAGAGCATCAGGACTTTTTGAAGATACTGCCACTTCTACCTATCTTTTCTAACTAGAACTTGCAGTATTTCTGTTTTTTAACATGGCGTTGCAGAATATTGGTGCCTCAAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGCAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAGGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTCGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACACGAGACAGCAAAGCTGGCTGGTCTTCTTGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACATTTATTCTCAAGAACCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATGTTCTAAAAAAGAAAAGCACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGACGAAGATGATGATGACGTGCAGTGGCAAACGGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCAGATATGGTCATGCTCTCAACTACTGAAGAGAACAAGTCGTCAAAGAAGTCATCTGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGAGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTGCATCCACAACCGATCTCAAATCATTCTTGGAATCACTCGCTGGAACTCGCCAAGAGATTGTGAACGCCTTAGTCGAAGCTCTATTTGATGGTGTTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAGAAGAAGAAGTTCCTGGCTGTAGTTGCTGCTGTGGAAGGATCACAGATAATTCTTTTACGTGCCATTGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCACTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCATTCATCGAATGGCTCCAAAATGCTGAGTCGGAGACGGAAGAAGAGTAAGAATAATATTACTGCATGCCTTTTCGTTATGCTATCTCTCTGCCTATGCGTTGGGTTTTAAATTTTACTGTCATAGTTTTTCGTTTAGCATTTCTGGAATAAGGGATGATCGTGTTGTGTTCTCATGTCTACGTGTTCAATTTGGGTGTCTTTGGGTTTATATGAACCTTGTCTTTGAACAATGGTATTGTATTTGTTTTTTCTTCAACTAGTAGCGTGTCTCTTATAAAAGCACTTCTTTAGAACTGCTGTTTAGTTTTTATTGAAAAATGGTATTGCTATTTCTTGTTTCTTTAGATCTGCTGTTTAATTATTATTTTACTTCATAAACTAACTTTTAACATTTTGTT

Coding sequence (CDS)

ATGGCGTTGCAGAATATTGGTGCCTCAAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGCAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAGGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTCGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACACGAGACAGCAAAGCTGGCTGGTCTTCTTGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACATTTATTCTCAAGAACCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATGTTCTAAAAAAGAAAAGCACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGACGAAGATGATGATGACGTGCAGTGGCAAACGGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCAGATATGGTCATGCTCTCAACTACTGAAGAGAACAAGTCGTCAAAGAAGTCATCTGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGAGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTGCATCCACAACCGATCTCAAATCATTCTTGGAATCACTCGCTGGAACTCGCCAAGAGATTGTGAACGCCTTAGTCGAAGCTCTATTTGATGGTGTTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAGAAGAAGAAGTTCCTGGCTGTAGTTGCTGCTGTGGAAGGATCACAGATAATTCTTTTACGTGCCATTGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCACTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCATTCATCGAATGGCTCCAAAATGCTGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEEE

Homology

[Close]

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot
Match: P48724 (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.5e-159
Identity = 306/448 (68.30%), Postives = 356/448 (79.46%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGA N DDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAK L RPASYTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTS VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDVLKKKSTTTKDAVTK-VPSKKKNNGSDED-HSPTRSHADDNDRAADDEDDDD--VQW 240
           KK+V KK S++ KD   K   SKKK +GSDED  SPT    ++ + A D++DDDD  VQW
Sbjct: 181 KKEVKKKGSSSAKDGTAKSTISKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDDGVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG 300
            TDTSL+A++QRI+EQLSAVTADMVML+T E  K  K +S     + NGN        +G
Sbjct: 241 LTDTSLDASRQRIKEQLSAVTADMVMLTTDEPEKKKKAASNQNGGSQNGNS-----KNYG 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            ++ E+K  LKKG   ++L S L +L    QE ++ALVEALF+G  KGF +E +KKK + 
Sbjct: 301 TVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKKKNYF 360

Query: 361 AVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
           A   A EGSQI+LL AIE FC   +  A KEVALVLK+LYD DV+EEE ++ WYQ+G+ G
Sbjct: 361 AAAVAEEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWYQKGLKG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 445
            NK+S+IWK  +PFI+WLQNAESE++EE
Sbjct: 421 DNKNSKIWKNAQPFIDWLQNAESESDEE 443

[Close]

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot
Match: Q9C8F1 (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 3.1e-155
Identity = 303/453 (66.89%), Postives = 361/453 (79.69%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDVL-KKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK+   KKK+ TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            L+ EIK+ L  G+S T LK+ L S +   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AVVAAVE-----GSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQ 420
             +  ++      +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++EWY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 445
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot
Match: P55876 (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 9.0e-155
Identity = 309/454 (68.06%), Postives = 358/454 (78.85%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKN--NGSDEDH--SPTRSHADDNDRAADDEDDDDVQW 240
           KKD  KK S   KD+  K   KK     GSDEDH  SPTRSH  D D+AA D+DDDDVQW
Sbjct: 181 KKDAKKKGS---KDSTAKGLKKKATTATGSDEDHSSSPTRSH--DGDKAAADDDDDDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNG------- 300
           QTDTS+EAAKQR+QEQLSA TA+MVMLS TEE +   K   H+  + NG+          
Sbjct: 241 QTDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKPA 300

Query: 301 -NGVSTHGALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKE 360
               S +  LI +IK  L    + + LK+ L S     Q+++NA +EALF GVGKGF+KE
Sbjct: 301 VTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTKE 360

Query: 361 VVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLE 420
           VVK KK+LAV    EG+Q +L++AIE+F    +PEA KEV +VLK+LYDGD+++EE +++
Sbjct: 361 VVKNKKYLAVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEETIVD 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETE 443
           WY   VA A K SQ+ K  KPF+EWLQ+AES+ E
Sbjct: 421 WYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

[Close]

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot
Match: Q9S825 (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.6e-151
Identity = 304/449 (67.71%), Postives = 358/449 (79.73%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQTD 240
           K    KK  +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQTD
Sbjct: 181 K--AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           TS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       L
Sbjct: 241 TSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKNL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           +Q++K+YLKKG+  + LKSF+ SL+   Q+I++AL  ALFDGVGKGF+KEV KKK +LA 
Sbjct: 301 VQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLAA 360

Query: 361 VAAV--EGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVA 420
            A +  +GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+WY++G+ 
Sbjct: 361 AATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLT 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 445
           GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 GADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot
Match: P59325 (Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 9.0e-46
Identity = 149/450 (33.11%), Postives = 218/450 (48.44%), Query Frame = 0


Query: 13  DAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEK 72
           D FYRYKMP+++ K+EG+GNGIKT +VNMV++AKAL RP +Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMITMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDVLKKKSTT 192
           +D   KL TFILKNPPE       KK                 E++KK +K         
Sbjct: 131 LDTHHKLCTFILKNPPENSDIGTGKK-----------------EKEKKNRK--------- 190

Query: 193 TKDAVTKVPSKKKNNGS---DEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQR 252
                     K K NGS    E   P   +      A ++E+DDD  W  DT+ EA ++R
Sbjct: 191 ---------GKDKENGSVSTSETPPPPPPNEISPPHAVEEEEDDD--WGEDTTEEAQRRR 250

Query: 253 IQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKK 312
           + E              ++  K    S + E++                 +  + D++KK
Sbjct: 251 MDE-------------ISDHAKGLTLSDDLERTVEE-------------RVNILFDFVKK 310

Query: 313 GASTTDLKSFLESLAGTRQEI-VNA-----LVEALFDGVGKGFSKEVVKK--KKFLAVVA 372
                 + S  + +    + + V A     L E LFD       +E +KK  + FL    
Sbjct: 311 KKEEGIIDSSDKEIVAEAERLDVKAMGPLVLTEVLFDEK----IREQIKKYRRHFLRFCH 370

Query: 373 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQG---VAGA 432
             + +Q  LL  +E        +   ++  +LK +YD D++EEE ++ W ++        
Sbjct: 371 NNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSK 393

Query: 433 NKSSQIWKYVKPFIEWLQNAESET---EEE 445
             + +I    +PFI+WL+ AE E+   EEE
Sbjct: 431 ELAKEIRVKAEPFIKWLKEAEEESSGGEEE 393

[Close]

BLAST of Cucsat.G4163 vs. NCBI nr
Match: XP_004150695.1 (eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 eukaryotic translation initiation factor 5 [Cucumis sativus] >KAE8652938.1 hypothetical protein Csa_017777 [Cucumis sativus])

HSP 1 Score: 838 bits (2165), Expect = 1.03e-305
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS
Sbjct: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. NCBI nr
Match: XP_008445359.1 (PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_008445360.1 PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo])

HSP 1 Score: 817 bits (2111), Expect = 1.75e-297
Identity = 432/444 (97.30%), Postives = 440/444 (99.10%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           A EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 AEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. NCBI nr
Match: KAA0064817.1 (eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 816 bits (2109), Expect = 3.53e-297
Identity = 431/444 (97.07%), Postives = 439/444 (98.87%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           + EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 SEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. NCBI nr
Match: XP_038884689.1 (eukaryotic translation initiation factor 5-like [Benincasa hispida])

HSP 1 Score: 798 bits (2061), Expect = 7.84e-290
Identity = 424/445 (95.28%), Postives = 431/445 (96.85%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKA+RRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKALRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKST-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKST TTKDAVTKVPSKKKNNGSDEDHSP RSHA DND+AADD+DDDDVQWQTD
Sbjct: 181 KKDSLKKKSTGTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDQAADDDDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENK SKKS EHEKSA NGNKNGNGVSTHGALI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKLSKKSPEHEKSAVNGNKNGNGVSTHGALI 300

Query: 301 QEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360
           QEIKDYLKKG S  DLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA  
Sbjct: 301 QEIKDYLKKGPSAADLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLASA 360

Query: 361 AAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANK 420
           AA EGSQIILLRAIESFC+NTSPEAGKEVALVLKSLYDGDVI+EEFVLEWYQQGVAGANK
Sbjct: 361 AAEEGSQIILLRAIESFCINTSPEAGKEVALVLKSLYDGDVIDEEFVLEWYQQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 444
           SS IWK+VKPFIEWLQNAESETEEE
Sbjct: 421 SSHIWKHVKPFIEWLQNAESETEEE 445

[Close]

BLAST of Cucsat.G4163 vs. NCBI nr
Match: XP_022131574.1 (eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131575.1 eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131576.1 eukaryotic translation initiation factor 5-like [Momordica charantia])

HSP 1 Score: 768 bits (1983), Expect = 6.69e-278
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADD-EDDDDVQWQ 240
           KKD +KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AADD ED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKG+S  +LKSFLESL+GTRQE+VNALVE LFDGVGKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 VVAAVEGS-QIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
             AA EGS QIILLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVLEWYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL
Match: A0A0A0LMB4 (W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=1)

HSP 1 Score: 838 bits (2165), Expect = 5.01e-306
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS
Sbjct: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL
Match: A0A1S3BCI3 (eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC103488416 PE=3 SV=1)

HSP 1 Score: 817 bits (2111), Expect = 8.48e-298
Identity = 432/444 (97.30%), Postives = 440/444 (99.10%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           A EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 AEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL
Match: A0A5A7VCS9 (Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001250 PE=3 SV=1)

HSP 1 Score: 816 bits (2109), Expect = 1.71e-297
Identity = 431/444 (97.07%), Postives = 439/444 (98.87%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           + EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 SEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

[Close]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL
Match: A0A6J1BQ30 (eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 GN=LOC111004721 PE=3 SV=1)

HSP 1 Score: 768 bits (1983), Expect = 3.24e-278
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADD-EDDDDVQWQ 240
           KKD +KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AADD ED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKG+S  +LKSFLESL+GTRQE+VNALVE LFDGVGKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 VVAAVEGS-QIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
             AA EGS QIILLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVLEWYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL
Match: A0A6J1HCN3 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111462865 PE=3 SV=1)

HSP 1 Score: 754 bits (1948), Expect = 6.68e-273
Identity = 400/448 (89.29%), Postives = 418/448 (93.30%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQT 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +ND+AADD+DDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           + EIKDYLKK  S  DLKSFLESL GT QE  NALVEALF+GVGKGFSKEVVKKKKFL  
Sbjct: 301 VHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAV--EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
           VAA   EGSQI+LLR+IESFCLNT PE  KE+ALVLKSLYDGDVIEEEFVLEWYQQG+AG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444
           ANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

[Close]

BLAST of Cucsat.G4163 vs. TAIR 10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 549.7 bits (1415), Expect = 2.2e-156
Identity = 303/453 (66.89%), Postives = 361/453 (79.69%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDVL-KKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK+   KKK+ TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            L+ EIK+ L  G+S T LK+ L S +   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AVVAAVE-----GSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQ 420
             +  ++      +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++EWY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 445
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]

BLAST of Cucsat.G4163 vs. TAIR 10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 304/449 (67.71%), Postives = 358/449 (79.73%), Query Frame = 0


Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQTD 240
           K    KK  +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQTD
Sbjct: 181 K--AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           TS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       L
Sbjct: 241 TSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKNL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           +Q++K+YLKKG+  + LKSF+ SL+   Q+I++AL  ALFDGVGKGF+KEV KKK +LA 
Sbjct: 301 VQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLAA 360

Query: 361 VAAV--EGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVA 420
            A +  +GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+WY++G+ 
Sbjct: 361 AATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLT 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 445
           GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 GADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]

BLAST of Cucsat.G4163 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0


Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

[Close]

BLAST of Cucsat.G4163 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0


Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 156 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 215

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 216 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 246

[Close]

BLAST of Cucsat.G4163 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0


Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

The following BLAST results are available for this feature:

BLAST of Cucsat.G4163 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P48724	3.5e-159	68.30	Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5...	[more]
Q9C8F1	3.1e-155	66.89	Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX...	[more]
P55876	9.0e-155	68.06	Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1	[more]
Q9S825	1.6e-151	67.71	Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX...	[more]
P59325	9.0e-46	33.11	Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1...	[more]

BLAST of Cucsat.G4163 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150695.1	1.03e-305	100.00	eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 euk...	[more]
XP_008445359.1	1.75e-297	97.30	PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_00...	[more]
KAA0064817.1	3.53e-297	97.07	eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa]	[more]
XP_038884689.1	7.84e-290	95.28	eukaryotic translation initiation factor 5-like [Benincasa hispida]	[more]
XP_022131574.1	6.69e-278	91.29	eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131...	[more]

BLAST of Cucsat.G4163 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMB4	5.01e-306	100.00	W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=...	[more]
A0A1S3BCI3	8.48e-298	97.30	eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7VCS9	1.71e-297	97.07	Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1...	[more]
A0A6J1BQ30	3.24e-278	91.29	eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 G...	[more]
A0A6J1HCN3	6.68e-273	89.29	probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3...	[more]

BLAST of Cucsat.G4163 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36730.1	2.2e-156	66.89	Translation initiation factor IF2/IF5	[more]
AT1G77840.1	1.1e-152	67.71	Translation initiation factor IF2/IF5	[more]
AT5G20920.1	3.8e-07	29.03	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.2	3.8e-07	29.03	eukaryotic translation initiation factor 2 beta subunit	[more]
AT5G20920.3	3.8e-07	29.03	eukaryotic translation initiation factor 2 beta subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 159..179
None	No IPR available	GENE3D	2.20.25.350		coord: 105..145 e-value: 1.6E-19 score: 71.2
None	No IPR available	GENE3D	3.30.30.170		coord: 2..104 e-value: 9.0E-43 score: 147.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 266..293
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 148..223
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 143..235
None	No IPR available	PANTHER	PTHR23001	EUKARYOTIC TRANSLATION INITIATION FACTOR	coord: 1..444
None	No IPR available	PANTHER	PTHR23001:SF28	EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATED	coord: 1..444
None	No IPR available	CDD	cd11561	W2_eIF5	coord: 293..442 e-value: 3.95609E-36 score: 128.503
IPR003307	W2 domain	SMART	SM00515	542_3	coord: 353..440 e-value: 1.3E-30 score: 117.7
IPR003307	W2 domain	PFAM	PF02020	W2	coord: 366..444 e-value: 1.1E-19 score: 70.4
IPR003307	W2 domain	PROSITE	PS51363	W2	coord: 288..444 score: 23.980349
IPR002735	Translation initiation factor IF2/IF5	SMART	SM00653	eIF2Bneu4	coord: 15..129 e-value: 9.2E-63 score: 224.5
IPR002735	Translation initiation factor IF2/IF5	PFAM	PF01873	eIF-5_eIF-2B	coord: 11..127 e-value: 6.1E-37 score: 126.1
IPR016021	MIF4G-like domain superfamily	GENE3D	1.25.40.180		coord: 292..444 e-value: 4.6E-27 score: 96.8
IPR016189	Translation initiation factor IF2/IF5, N-terminal	SUPERFAMILY	100966	Translation initiation factor 2 beta, aIF2beta, N-terminal domain	coord: 10..101
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 298..438
IPR016190	Translation initiation factor IF2/IF5, zinc-binding	SUPERFAMILY	75689	Zinc-binding domain of translation initiation factor 2 beta	coord: 98..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G4163.T1	Cucsat.G4163.T1	mRNA
Cucsat.G4163.T2	Cucsat.G4163.T2	mRNA
Cucsat.G4163.T4	Cucsat.G4163.T4	mRNA
Cucsat.G4163.T6	Cucsat.G4163.T6	mRNA
Cucsat.G4163.T5	Cucsat.G4163.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001732	formation of cytoplasmic translation initiation complex
biological_process	GO:0001731	formation of translation preinitiation complex
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005829	cytosol
molecular_function	GO:0071074	eukaryotic initiation factor eIF2 binding
molecular_function	GO:0005092	GDP-dissociation inhibitor activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0005515	protein binding