Cucsat.G412 (gene) Cucumber (B10) v3

Overview
NameCucsat.G412
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionInhibitor_I29 domain-containing protein
Locationctg1: 8597750 .. 8598463 (-)
RNA-Seq ExpressionCucsat.G412
SyntenyCucsat.G412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACATGAAAATGTTAGTAGCACTTCGTTCGTATTTCATTCGCAATCGAGGTCTGGAAGAACGCATCCTTCGTCACGTAATACAAGAAGAACAAGATTTTATAAGGAAATTGGAGGTATACTATAGACATTTTTTTTGTTCTGATTTCCTTGTGAATATTATTATTGGGATTGACGAATATATATATATATATATATATATATATATATATATACATAGCTGGATGAAGCACAAGGTTTGAAGCCGGATGTTGTGGATTCAACTCTCGAACAAGATTTCAAGGAGGCACAAGGTTTGAAGCCGGATGTTGTGGAATCAACCCTGGAACAAGATTTCAAGGAGGATTTTGTTAAGACAGACAAGCCATGGGTGAGAACGGTTGGCATGACATTACCTGATTTTTTTTTTTATCGGTTCAGTTCGGCGGTTGATCCAGAATTGGATTTTCCCCATCTGGCCTGCACATACCCCCAACCAGTTCAACGTCAAGCTATACTTTTTTTTGTTCTGATACCAGGTAGCTTTTATATATTAATATATACTTTTTCTGTGTGTAAGGGATTACTGGAAGATGCAGAACGTTCTAAGGATTGGAAGAAGTTCGCTTCATGGATGTCGGAGCACAAGAAGAAGTACGAGAGCGATGAAGAGAAGTTGTATAGGTTTGGGATATTCAGAGGAGAACTGAAACATATTAAGAAGTTGAACAAGGAGGACAATGGATGTACCTTTGGGTTGAATCAGTACTCAGACCTAACCAATTCTGAATTTAACAGACTCGTA

Coding sequence (CDS)

AACATGAAAATGTTAGTAGCACTTCGTTCGTATTTCATTCGCAATCGAGGTCTGGAAGAACGCATCCTTCGTCACGTAATACAAGAAGAACAAGATTTTATAAGGAAATTGGAGCTGGATGAAGCACAAGGTTTGAAGCCGGATGTTGTGGATTCAACTCTCGAACAAGATTTCAAGGAGGCACAAGGTTTGAAGCCGGATGTTGTGGAATCAACCCTGGAACAAGATTTCAAGGAGGATTTTGTTAAGACAGACAAGCCATGGGGATTACTGGAAGATGCAGAACGTTCTAAGGATTGGAAGAAGTTCGCTTCATGGATGTCGGAGCACAAGAAGAAGTACGAGAGCGATGAAGAGAAGTTGTATAGGTTTGGGATATTCAGAGGAGAACTGAAACATATTAAGAAGTTGAACAAGGAGGACAATGGATGTACCTTTGGGTTGAATCAGTACTCAGACCTAACCAATTCTGAATTTAACAGACTCGTA

Protein sequence

NMKMLVALRSYFIRNRGLEERILRHVIQEEQDFIRKLELDEAQGLKPDVVDSTLEQDFKEAQGLKPDVVESTLEQDFKEDFVKTDKPWGLLEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNRLV
Homology
BLAST of Cucsat.G412 vs. ExPASy Swiss-Prot
Match: O65493 (Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.8e-10
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 119
           F SWMSEH K Y+S EEK++RF +FR  L HI + N E N    GLN+++DLT+ EF
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEF 107

BLAST of Cucsat.G412 vs. ExPASy Swiss-Prot
Match: P05994 (Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3)

HSP 1 Score: 65.9 bits (159), Expect = 3.7e-10
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 119
           F SWM +H K Y++ +EKLYRF IF+  LK+I + NK  NG   GLN++SDL+N EF
Sbjct: 48  FNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDEF 104

BLAST of Cucsat.G412 vs. ExPASy Swiss-Prot
Match: P10056 (Caricain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 4.8e-10
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFN 120
           F SWM  H K YE+ +EKLYRF IF+  L +I + NK++N    GLN+++DL+N EFN
Sbjct: 48  FNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDEFN 105

BLAST of Cucsat.G412 vs. ExPASy Swiss-Prot
Match: P14080 (Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-09
Identity = 30/59 (50.85%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 121
           F SWM +H K YES +EK+YRF IFR  L +I + NK++N    GLN ++DL+N EF +
Sbjct: 48  FDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKKNNSYWLGLNGFADLSNDEFKK 106

BLAST of Cucsat.G412 vs. ExPASy Swiss-Prot
Match: O23791 (Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 5.3e-09
Identity = 27/60 (45.00%), Postives = 43/60 (71.67%), Query Frame = 0

Query: 60  KKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLN-KEDNGCTFGLNQYSDLTNSEF 119
           K+F  WM+E+ + Y+ D+EK+ RF IF+  +KHI+  N + +N  T G+NQ++D+T SEF
Sbjct: 35  KRFEEWMAEYGRVYKDDDEKMRRFQIFKNNVKHIETFNSRNENSYTLGINQFTDMTKSEF 94

BLAST of Cucsat.G412 vs. NCBI nr
Match: KAE8650303.1 (hypothetical protein Csa_010836 [Cucumis sativus])

HSP 1 Score: 205 bits (522), Expect = 1.42e-63
Identity = 120/202 (59.41%), Postives = 120/202 (59.41%), Query Frame = 0

Query: 1   LRHVIQEEQDFIRKLELDEAQGLKPDVVDSTLEQDFKE-------------------DFV 60
           LRHVIQEEQDFIRKLELDEAQGLKPDVVDSTLEQDFKE                   DFV
Sbjct: 22  LRHVIQEEQDFIRKLELDEAQGLKPDVVDSTLEQDFKEAQGLKPDVVESTLEQDFKEDFV 81

Query: 61  KTDKPW------------------------------------------------------ 120
           KTDKPW                                                      
Sbjct: 82  KTDKPWVRTVGMTLPDFFFYRFSSAVDPELDFPHLACTYPQPVQRQAILFFVLIPGSFYI 141

BLAST of Cucsat.G412 vs. NCBI nr
Match: XP_038887495.1 (pro-cathepsin H-like [Benincasa hispida])

HSP 1 Score: 88.2 bits (217), Expect = 1.45e-19
Identity = 45/82 (54.88%), Postives = 55/82 (67.07%), Query Frame = 0

Query: 36  FKEDFVKTDKPWGLLEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKK 95
           FK D V++      + DA  S+DW+ F SWMS H KKY S+EE LYRFG+F+  LK I+K
Sbjct: 42  FKSDGVES------IYDAVLSEDWESFKSWMSMHNKKYGSEEEMLYRFGLFKKTLKRIEK 101

Query: 96  LNKEDNGCTFGLNQYSDLTNSE 117
           LNK   GCTFG N +SDLT  E
Sbjct: 102 LNKNYTGCTFGFNHFSDLTFDE 117

BLAST of Cucsat.G412 vs. NCBI nr
Match: KAF8405593.1 (hypothetical protein HHK36_010500 [Tetracentron sinense])

HSP 1 Score: 71.2 bits (173), Expect = 1.23e-11
Identity = 31/59 (52.54%), Postives = 45/59 (76.27%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 120
           F SWMS+H+K YES EEKL+RF +F+G L+HI + NK+ +    GLN+++DL+N EF +
Sbjct: 49  FESWMSKHEKMYESIEEKLHRFEVFKGNLEHIDETNKKVSSYWLGLNEFADLSNEEFKK 107

BLAST of Cucsat.G412 vs. NCBI nr
Match: KAE8057439.1 (hypothetical protein FH972_014132 [Carpinus fangiana])

HSP 1 Score: 68.6 bits (166), Expect = 1.27e-11
Identity = 31/57 (54.39%), Postives = 42/57 (73.68%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 118
           F SWMS+H K YES EEKL+RF IF+  LKHI + NK+ +    GLN+++DL++ EF
Sbjct: 48  FESWMSKHSKSYESVEEKLHRFDIFKDNLKHIDETNKKVSNYWLGLNEFADLSHEEF 104

BLAST of Cucsat.G412 vs. NCBI nr
Match: AAU07805.1 (proteinase omega, partial [Carica papaya])

HSP 1 Score: 66.6 bits (161), Expect = 6.47e-11
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFN 119
           F SWM  H K YE+ +EKLYRF IF+  L +I + NK++N    GLN+++DL+N EFN
Sbjct: 48  FNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDEFN 105

BLAST of Cucsat.G412 vs. ExPASy TrEMBL
Match: A0A0A0L4P3 (Inhibitor_I29 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G131910 PE=4 SV=1)

HSP 1 Score: 196 bits (498), Expect = 4.94e-61
Identity = 117/212 (55.19%), Postives = 119/212 (56.13%), Query Frame = 0

Query: 1   LRHVIQEEQDFIRKLEL-----------------------------DEAQGLKPDVVDST 60
           LRHVIQEEQDFIRKLE+                              EAQGLKPDVV+ST
Sbjct: 20  LRHVIQEEQDFIRKLEVYYRHFFCSDFLVNIIIGIDEYISTLEQDFKEAQGLKPDVVEST 79

Query: 61  LEQDFKEDFVKTDKPW-------------------------------------------- 120
           LEQDFKEDFVKTDKPW                                            
Sbjct: 80  LEQDFKEDFVKTDKPWVRTVGMTLPDFFFYRFSSAVDPELDFPHLACTYPQPVQRQAILF 139

BLAST of Cucsat.G412 vs. ExPASy TrEMBL
Match: A0A5N6R8Z3 (Inhibitor_I29 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_014132 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 6.16e-12
Identity = 31/57 (54.39%), Postives = 42/57 (73.68%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 118
           F SWMS+H K YES EEKL+RF IF+  LKHI + NK+ +    GLN+++DL++ EF
Sbjct: 48  FESWMSKHSKSYESVEEKLHRFDIFKDNLKHIDETNKKVSNYWLGLNEFADLSHEEF 104

BLAST of Cucsat.G412 vs. ExPASy TrEMBL
Match: Q670P1 (Proteinase omega (Fragment) OS=Carica papaya OX=3649 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.13e-11
Identity = 30/58 (51.72%), Postives = 41/58 (70.69%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFN 119
           F SWM  H K YE+ +EKLYRF IF+  L +I + NK++N    GLN+++DL+N EFN
Sbjct: 48  FNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDEFN 105

BLAST of Cucsat.G412 vs. ExPASy TrEMBL
Match: A0A7J9K832 (Inhibitor_I29 domain-containing protein (Fragment) OS=Gossypium armourianum OX=34283 GN=Goarm_002442 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.13e-11
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 120
           F SW+S+H K YES EEKL RF +F+  LKHI K NKE +    GLN+++DL++ EF +
Sbjct: 49  FESWISQHGKIYESIEEKLMRFEVFKDNLKHIDKRNKEISSYWLGLNEFADLSHDEFKK 107

BLAST of Cucsat.G412 vs. ExPASy TrEMBL
Match: A0A7J8VW64 (Inhibitor_I29 domain-containing protein (Fragment) OS=Gossypium klotzschianum OX=34286 GN=Goklo_003061 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.13e-11
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 120
           F SW+S+H K YES EEKL RF +F+  LKHI K NKE +    GLN+++DL++ EF +
Sbjct: 49  FESWISKHGKVYESIEEKLMRFEVFKDNLKHIDKRNKEISSYWLGLNEFADLSHDEFKK 107

BLAST of Cucsat.G412 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 66.2 bits (160), Expect = 2.0e-11
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 119
           F SWMSEH K Y+S EEK++RF +FR  L HI + N E N    GLN+++DLT+ EF
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEF 107

BLAST of Cucsat.G412 vs. TAIR 10
Match: AT4G35350.2 (xylem cysteine peptidase 1 )

HSP 1 Score: 66.2 bits (160), Expect = 2.0e-11
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 119
           F SWMSEH K Y+S EEK++RF +FR  L HI + N E N    GLN+++DLT+ EF
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEF 107

BLAST of Cucsat.G412 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 57.4 bits (137), Expect = 9.3e-09
Identity = 25/59 (42.37%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 121
           F +W+S  +K YE+ EEK  RF +F+  LKHI + NK+      GLN+++DL++ EF +
Sbjct: 51  FENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKK 109

BLAST of Cucsat.G412 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 3.3e-06
Identity = 24/62 (38.71%), Postives = 35/62 (56.45%), Query Frame = 0

Query: 59  WKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEF 118
           W+ +  W S H     S EEK  RF +F+  +KHI + NK+D      LN++ D+T+ EF
Sbjct: 35  WELYERWRSHHTVA-RSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEF 94

Query: 119 NR 121
            R
Sbjct: 95  RR 95

BLAST of Cucsat.G412 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 47.4 bits (111), Expect = 9.6e-06
Identity = 23/59 (38.98%), Postives = 35/59 (59.32%), Query Frame = 0

Query: 62  FASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNGCTFGLNQYSDLTNSEFNR 121
           F+ +  +  K Y S+EE  YRF +F+  L+  ++  K D   T G+ Q+SDLT SEF +
Sbjct: 51  FSLFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRK 109

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O654932.8e-1054.39Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1[more]
P059943.7e-1054.39Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3[more]
P100564.8e-1051.72Caricain OS=Carica papaya OX=3649 PE=1 SV=2[more]
P140801.1e-0950.85Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2[more]
O237915.3e-0945.00Fruit bromelain OS=Ananas comosus OX=4615 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAE8650303.11.42e-6359.41hypothetical protein Csa_010836 [Cucumis sativus][more]
XP_038887495.11.45e-1954.88pro-cathepsin H-like [Benincasa hispida][more]
KAF8405593.11.23e-1152.54hypothetical protein HHK36_010500 [Tetracentron sinense][more]
KAE8057439.11.27e-1154.39hypothetical protein FH972_014132 [Carpinus fangiana][more]
AAU07805.16.47e-1151.72proteinase omega, partial [Carica papaya][more]
Match NameE-valueIdentityDescription
A0A0A0L4P34.94e-6155.19Inhibitor_I29 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G1319... [more]
A0A5N6R8Z36.16e-1254.39Inhibitor_I29 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_... [more]
Q670P13.13e-1151.72Proteinase omega (Fragment) OS=Carica papaya OX=3649 PE=3 SV=1[more]
A0A7J9K8323.13e-1152.54Inhibitor_I29 domain-containing protein (Fragment) OS=Gossypium armourianum OX=3... [more]
A0A7J8VW643.13e-1152.54Inhibitor_I29 domain-containing protein (Fragment) OS=Gossypium klotzschianum OX... [more]
Match NameE-valueIdentityDescription
AT4G35350.12.0e-1154.39xylem cysteine peptidase 1 [more]
AT4G35350.22.0e-1154.39xylem cysteine peptidase 1 [more]
AT1G20850.19.3e-0942.37xylem cysteine peptidase 2 [more]
AT5G50260.13.3e-0638.71Cysteine proteinases superfamily protein [more]
AT4G39090.19.6e-0638.98Papain family cysteine protease [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 103..159
e-value: 8.8E-21
score: 85.0
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 103..159
e-value: 2.6E-17
score: 63.0
NoneNo IPR availableGENE3D1.10.287.2250coord: 72..163
e-value: 1.7E-20
score: 74.8
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 99..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G412.T1Cucsat.G412.T1mRNA
Cucsat.G412.T3Cucsat.G412.T3mRNA
Cucsat.G412.T2Cucsat.G412.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008233 peptidase activity