Cucsat.G3764 (gene) Cucumber (B10) v3

Overview
NameCucsat.G3764
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionWAT1-related protein
Locationctg105: 638893 .. 642446 (+)
RNA-Seq ExpressionCucsat.G3764
SyntenyCucsat.G3764
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACATCATTATTTTTTGGCTTTGAAAAGAAAAAAAAAACAAAGTTTGATAATAATGATAATGAATGAGAGAGTTTTATGGAATGGTTTGCCCACCATTGTGATGATGTTGGTTCAATTTGGGTTTGCTGGAGTTAACATTTTCTATAAATTAGCTGCTGCTGATGGAATGAGTTTTCGGATTATTATTGCTTACCGTTTCTTATTTGCTTCTGCTTTCATTCTTCCCATTGCATTCTTTCTGGAAAGGTACTTAATACTTCCTTTCTTTTCTCATTTTCTTTCCCCCACACAAATAAACTGTTGTTTTTATTTTATTTTATTGTTTTCAGAGGTAGAAGACCGAAGCTTACTTGGTCCGTTATTTTCTATGCATTTCTTTGTGGACTCTTTGGGTAATTAATAATTCAGTTTCTTATATTTATTTTGAATTAAACAGAGATTTTGATGTAACTCTAACTTCATCCACCCGTGGATATGGAACTGTCAACAGTTAGCCGTTCAATTATATGCCTACCTCTCTGCTTAGCATCTCATCATTTCTTGTGATTTCCTACCAATATTCCGCCCTCTTAAGTTCTTAAGATTATGTGATTTTTTCCATGCATAAACCTTTTTTTTCTTTTTACAAATTAAATAACACTTGTTTTTCACTCCACTCCAACTTTTGTTTCATCTCTCACTTTTCTTAAATTTTATCTAGACTAAGTTAAAAATGGCAATTTATACCTATTTTTTTTCTTCTAACTTTAAATTAAAATGGAAAATAGATTTTAAAAATATATAATATTGAATATCTTATGTTCTACGCATTTTTTGATATTTATTTCACAAATTCAAATTATTGTTCAAAGTGTTGAAATAATACTTTTATTTTTATATATTTAGATTTAAAAAAAAAAATATAACAAATCTTCACAATTATATTTATACTATATAATAATTTGAAAAAAACTCACAAACTCACCATGAAAAAATACGTCGATCGGGTATGAAAGGATGCACGATCTTGCGTGCACAATTTATATGATACGCGATCGTATACAAAATGATACACGATCGCATAGCAAATCTAGATGATTTTGGTTTAAGATTGTGTATAAAGATCTAAACAATTTTGTACCAAATATCGTTAAGATTTGGTATACGATCATTTAGATTTAACTACGTAATCTTGAACCAAATAACATTTTGAAAAAAATAATAGAAGATTTATGATTGATTGAAAATAAATATCATGATTTCAAAAAAATATAAAAGAAAATTTGAAGATGAAGAGAAGAAGATGGAAAGAAATTGTAAAAGAAAGAAAATAGAAATGATGAATAAACTTAGAATATTTAAGAAAAATGACTCGTTTGAAGGTTTTTTGATTTTATTATACGGTCATAAATATTTGGATGTTTTTGTTAAATGGATGAAAATTAACCTTTAAAAAATCCTCCAAATATTTGTTTGAGTAAAAGTAAAGGAATAGTGCAAATTTAACCATTAGATTCAAAATAATTACGTGTATAGCAACATTTTAATAAATTTGCAAATATAGTAAAACTTGTCAAATTCTAATAATGATAGATGTTGCAAATATTGATCTATCAGTAATAAATCATACGAGTCTATTAGTGATACAAGTCTATTAACAATAGATTTTGCTATATTTACAATTTTTTTTAAAAAATATTACTATACACCTTATTATTATTTCTAAAATGGTCCTTTTTACTTCTGATGGAATAAAAAACGTGAATCTTTATTTTGGTGAAATTTATAAAAAAAAAATGATAAAAAAGAAAAAAGAAATGGGGAAGCCCTTTGAGGACAATAAAAGTGAGGCCATGGTAAGTTAGTTATTGGATTAGCTATAATTAAGAACATTAACAATTCAAAAATAAAATATATAATGTAATAGTGGTACAAATACTTGCATGCAGGGGATCACTGAGTCAAAATTTGTACGTGGAGAGTTTAGCTTTAACATCTGCAACATATGCTTCAGCCATTGGTAACCTTGCGCCAGCCATCACTTTCATTTTGGCCGTTTCCTTCAGGTTTTGTGTAATAAATATTTATTAATTTATGATATAACAAATGGAATTAATAATAACAATAAATAAGAAAGAAAAAGAAAAGGAATATTAGAATAATAATTATTCATGTTGATATATATATATATACGAATGGATGGGTCAGGCTGGAGAGGATGAACATAGGAACAATGCGAGGGAAGGCTAAAGTGATGGGAACATTAATAGGAATAGTTGGGGCCATGATTCTCACATTCTACAAAGGCGTGGAACTTCATCCTTGGTCTACTCGGGTCGACCTTTTGCATAAAGGTCATAATTCCACTGAACACGTGGCCCCAACAGAACATACAATTCATAGTCATGTGTTGGGGAGTGTGATGGGTGTTGGAAGCTGCTTCTCTTATGCTTTATGGCTCATCGTCCAGGTGAGTTTCACATCATCCCTCTCACGTTCAATCATGCTAGATTAAATTAAATTAGATTAGATTATGGTTTTTTTTTTTATCAATATTAATTTTATAGTATATATAGGCAAAAATGAGTGAATGCTATCCATGCCACTACTCAAGTACAGCATTAATGTGTATGATGGGATCCGTTCAAGCGGTTGGTTTTGCTCTGTGCGTCGAAACTCAATGGAGTCGTTGGAAATTGGGTTGGAATATCAGGCTTCTTTCTGTCGCATATACAGTATATCTTTTTTACTTTATCTCTTTCAAATTCTTCTATTTTTTCTTTTTAACAAAAGAGTTGTGTTTTCTCATTTTCGACTCAAATGATGTTCATCTTAATACCTTCTTTCTTCTACTTACAATCAAAGAATATATATATAAAACCAAACACTTCTTGTTCTTTACAAAGTTATCACACATCAATTTGTACTTGACATGGATAAGCTGCCAAGTGATGTAATAATATAATATAATTTTTTCATATAATATGGTTATGTTGCCTTAACCCTTCTATTATTTCTTCATTATACTTTTTAGTTGGATACCAATGCTATTTTGATTCACAATTTTTTTAACTAAGTGCTTTAAAAGTATTTATTTTAATTTTTGCAATTTTAAAAAGTGATCATTCATTTGGTCTCAAACAAAAAGATAAAGCAACTAAAACAATACCATTCAAACAAAAAGATAAAGCAACTAAAACAAACTAAAACAAACTAAAACAATACCATTCAAACAAAAAGATAAAGCAACTAAAACAATACCATTCGAAGAGGTTAAAATTATTATATTGTAAATAGGAAGAAGTGAAAATTTGTTAGTAGCCATTTTAAATGTATACATGGAGTAAAGTAATTGAAAGGAATATTTAAAAGCTTACCCGTTTTTAAGTAGGTTGAGGTTGATTGGATTTTAAGAACTTTTGTACAAAGTTGAAGAGAAAAGATATATAGAAAATTGAAAGAAAAAATATATATATACGGTGTTTTGTGATATATAATTAATAATGTGTATATGTACTGTGGATGATGGTAGGGGATCGTGGCTTCAGGAGTGATGGTAACGTTAATAACATGGTGCGTACGAATCAGAGGCCCAATGTTTGTATCAGTCTTCAGCCCTCTAATTCTAGTTCTCGTTGCCATAGCTGCCTCCTTGTTTCTACAAGAAAAACTCTACTTAGGATGGTATCTAATACTTCCCTCCTTTTTCAATTATATATCATTTTCGTATGATTAATTTTTACAAAAATAATAAACCGTCAAAATATTTAGGATTCCAATAACAAATAAAAAAAAGCTAGGGTTCCAATAACAAATAAAAAAGCTGACGAAGCTAATCATTTCCTTCTAAATATTTTACGTTTGCTTTTTTTCACTGTCTTTCATCTCCTCATTTTCTGCTATTTTTTTTCCTTTGTATGTCTTTTTCTCTTTTTCTTCTGTAATTTTTTTTTAAAATATTTCCTTTTGGCTTTCAATTTTTCTTTTCATTTTTATCATTTTTTTTCTCTTCTTCCAAAATTTTTCTTTAAACTCATTTAGTTGTTTAAGGCAGTGGATCAAATATAAAAAATCGTAAAAAAAAACCGTTTAAAATTCTTGGTTTTTAAAATTCTTTTTATATACGATCATTTAAATTTGGTTGGAAATATAATCGTAAAAAAAATAGTCAAATCTAAACAAAGAATCTTGGAAAAAAAATGGTTTAGATTTGGATAAAACGATAAAATTAAACGATCAAGTAAGAAATGAATAGTAAAATCTAAATGATTGTTTACTAAATATTTTACGTACTGCATGTCAATTAGTTGTGTGACTTTTTTGATATTTTTTATTGTAGCGTGTGAACTTTTTGTGTGTTTAAAATTGTTTTATACTGTGTAAATATTTCAACGTTATGTTATCCTTGTTTTTTAAAAAATATTTTTTCTTTTTTTCTTATCCAATTGATTTTTGCATTTAATTAGTGATATAATCACTGTTAATATTTCAACTCTACTGAAAACACTATATTTATCATTTATAAATTTGGAGGCATCTAAATATTTAAAATTTTCTATATTTCTAAATATTATTCTCAGTCATTTTTCTATTCAAAATAATTATACCATTATGTGTTTGTAAATTCTTTAATTTTCTCTTTTAGAAAAAACAATAAAGCAGTTTTCTAAATTCCATCTGGCACATTAAAATAAGCTAGCTAGGAACGGAGAATACATTTCCATAATTTCCATAATGTAGAGAGACACTGAATTAAAGATATGTGATTATTGTTGTTACACAAAGCAATAGCTGATCTATATTTTTTTCTTAGGCTCGAAAAAGAAAAAAAGAACACGAATTTCAAGTTATTGAAATTTTTTGGACTTTCTAGTTTGACGAAGATCCCGTTGTTAGTGGATATTAAAGCATTTCAAAATTGGATCAATTAATTGATGATTTTTTTACTGAAGTGCTGTTACAATGATTATGTTTGCTTTTGTAAGTGAATTTGTATTTTTCATCCGACGTGGTATCTAGCCTTGAGGTTATCGATACTTCATTGCATTACAATCATACAAAATTCATTTTTTTCCTTATAAAAAATGTTTTGAGAAACAATTTCCTAAAGATGGCAGCATGTAATTAGGTTAATTAGCCAAGAATAATAAATATAGAAAAGTTTATCATGAGTAGATTCGATTGGAGATATAATTGTAGGTATGGTTTTTCTTTAATAAACTCTACAAGTAATATATTTGTTAAAAGATATATGAATTTACATTTTTATCCCGTAAAAAAAAAAAAAAGATAACATTGTTATTTGGGCTAATTAAATTTTGTATTTATACATGTGCTTGTAATGAATGGCAGCGTGGTTGGAGGAATGTTGATGGTGTGTGGATTATATATGGTACTATGGGGTAAAAGCAAAGAAATCAGAAAGATAACACAATTAGCTCCCATGGAAAGCATTGAAGAGCAGCTGCAGCAGCTGGGTGGTGAAGGGATAGATTTGGTCATTTCCTCTCCAATTACTCCATTGTCCAAAACTCATGTCACCAATAGTCAGCATAATAATAATAGTAATGTTCCACATTCCAACAATTAGAAACCAATAAACACTTGTAATCTTGTATATT

Coding sequence (CDS)

CATCATTATTTTTTGGCTTTGAAAAGAAAAAAAAAACAAAGTTTGATAATAATGATAATGAATGAGAGAGTTTTATGGAATGGTTTGCCCACCATTGTGATGATGTTGGTTCAATTTGGGTTTGCTGGAGTTAACATTTTCTATAAATTAGCTGCTGCTGATGGAATGAGTTTTCGGATTATTATTGCTTACCGTTTCTTATTTGCTTCTGCTTTCATTCTTCCCATTGCATTCTTTCTGGAAAGAGGTAGAAGACCGAAGCTTACTTGGTCCGTTATTTTCTATGCATTTCTTTGTGGACTCTTTGGGGGATCACTGAGTCAAAATTTGTACGTGGAGAGTTTAGCTTTAACATCTGCAACATATGCTTCAGCCATTGGTAACCTTGCGCCAGCCATCACTTTCATTTTGGCCGTTTCCTTCAGGCTGGAGAGGATGAACATAGGAACAATGCGAGGGAAGGCTAAAGTGATGGGAACATTAATAGGAATAGTTGGGGCCATGATTCTCACATTCTACAAAGGCGTGGAACTTCATCCTTGGTCTACTCGGGTCGACCTTTTGCATAAAGGTCATAATTCCACTGAACACGTGGCCCCAACAGAACATACAATTCATAGTCATGTGTTGGGGAGTGTGATGGGTGTTGGAAGCTGCTTCTCTTATGCTTTATGGCTCATCGTCCAGGCAAAAATGAGTGAATGCTATCCATGCCACTACTCAAGTACAGCATTAATGTGTATGATGGGATCCGTTCAAGCGGTTGGTTTTGCTCTGTGCGTCGAAACTCAATGGAGTCGTTGGAAATTGGGTTGGAATATCAGGCTTCTTTCTGTCGCATATACAGGGATCGTGGCTTCAGGAGTGATGGTAACGTTAATAACATGGTGCGTACGAATCAGAGGCCCAATGTTTGTATCAGTCTTCAGCCCTCTAATTCTAGTTCTCGTTGCCATAGCTGCCTCCTTGTTTCTACAAGAAAAACTCTACTTAGGATGCGTGGTTGGAGGAATGTTGATGGTGTGTGGATTATATATGGTACTATGGGGTAAAAGCAAAGAAATCAGAAAGATAACACAATTAGCTCCCATGGAAAGCATTGAAGAGCAGCTGCAGCAGCTGGGTGGTGAAGGGATAGATTTGGTCATTTCCTCTCCAATTACTCCATTGTCCAAAACTCATGTCACCAATAGTCAGCATAATAATAATAGTAATGTTCCACATTCCAACAATTAG

Protein sequence

HHYFLALKRKKKQSLIIMIMNERVLWNGLPTIVMMLVQFGFAGVNIFYKLAAADGMSFRIIIAYRFLFASAFILPIAFFLERGRRPKLTWSVIFYAFLCGLFGGSLSQNLYVESLALTSATYASAIGNLAPAITFILAVSFRLERMNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTIHSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQWSRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFLQEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISSPITPLSKTHVTNSQHNNNSNVPHSNN
Homology
BLAST of Cucsat.G3764 vs. ExPASy Swiss-Prot
Match: Q4PT23 (WAT1-related protein At1g25270 OS=Arabidopsis thaliana OX=3702 GN=At1g25270 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.7e-47
Identity = 97/209 (46.41%), Postives = 144/209 (68.90%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           + +G+  G+AK++GTL+G  GA++  FYKG+E+H WST VDLL   H      A T H  
Sbjct: 119 LRLGSNEGRAKLVGTLLGACGALVFVFYKGIEIHIWSTHVDLLKGSHTGR---ATTNH-- 178

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           H  +LG +M +GS  S +LWL++QAK+ +     Y +T+LM  +GS+  V  ALC +  W
Sbjct: 179 HVSILGVLMVLGSNVSTSLWLLLQAKIGKELGGLYWNTSLMNGVGSLVCVIIALCSDHDW 238

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
            +W+LGW+I LL+  Y+GIV SG++V L+ WC+  +GP+FV+VFSP+ LV+VA+  S  L
Sbjct: 239 EQWQLGWDINLLATLYSGIVVSGMVVPLVAWCIATKGPLFVTVFSPIRLVIVALIGSFAL 298

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKE 210
           +E L+LG ++G M+MV G+Y+V+W K KE
Sbjct: 299 EEPLHLGSIIGAMIMVGGVYLVVWCKMKE 322

BLAST of Cucsat.G3764 vs. ExPASy Swiss-Prot
Match: F4HVM3 (WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 8.6e-44
Identity = 91/213 (42.72%), Postives = 141/213 (66.20%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           + +G+  G AKV GTL G+ GA++  FY+G+E+  WST V+L+++  +S+        T 
Sbjct: 119 VRLGSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDAT----TH 178

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           H  +LG+++  G   S +LW ++Q K+S+ +   Y +  LM MMG V A+  ALC E   
Sbjct: 179 HISILGALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDL 238

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
             W+LGWNIRLL++AY  I+ SG++V +  WC+  RGP+FVSVFSP+ LV+VA+  S  L
Sbjct: 239 DEWRLGWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLL 298

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKI 214
            E L+LG ++G +++V  LY+VLW K+KE++ +
Sbjct: 299 DETLHLGSIIGTVIIVGALYIVLWAKNKEMKSM 327

BLAST of Cucsat.G3764 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.6e-37
Identity = 88/225 (39.11%), Postives = 141/225 (62.67%), Query Frame = 0

Query: 8   GKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTIHSHVLGS 67
           G+AKV+GTL+ ++GAM+L+FY G  +    +++   +   N T+H + + H+  +  LG 
Sbjct: 131 GQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAY-AENITKHGSSSGHS--NFFLGP 190

Query: 68  VMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQWSRWKLGW 127
            + + +  S+A W I+Q KMSE +   Y+ST LMC+MGS+Q    AL  +   S W L  
Sbjct: 191 FLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLSS 250

Query: 128 NIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFLQEKLYLG 187
            +R +S  Y G+VAS +   L++W ++ +GP++VSVFSPL+LV+VAI +   L+EKLY G
Sbjct: 251 PLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYTG 310

Query: 188 CVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGE 233
             +G  L+V GLY VLWGK +E+ +  +    E +++Q  ++  E
Sbjct: 311 TFMGSALVVIGLYGVLWGKDREVSEKEE--EREKVKQQNHKVKSE 350

BLAST of Cucsat.G3764 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.3e-36
Identity = 95/266 (35.71%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELH-PWSTRVDLLHKGH--NSTEHVAPTE 60
           +NI  +R KAK++GTL+G+ GA+++T YKG  +  PWS        GH  NS +H     
Sbjct: 129 VNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDH----- 188

Query: 61  HTIHSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVE 120
              ++ V+G+++ +  C +++ + ++Q+   + YP   S +AL+C+ G+VQ+   AL VE
Sbjct: 189 ---NNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 248

Query: 121 TQWSRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAAS 180
              S W +GW+ RL +  YTGIV+SG+   +    ++ RGP+FV+ F+PL ++LVA+ AS
Sbjct: 249 RHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIAS 308

Query: 181 LFLQEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPME--SIEEQLQQLGGEGID 240
             L E+++ GCV+GG ++  GLYMV+WGK K+  +++ L  +E  S++E       E  +
Sbjct: 309 FILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDY-EVSGLDILEKNSLQELPITTKSEDDN 368

Query: 241 LVISSPITPLSKTHVTNSQHNNNSNV 262
            ++SS I+  S   +    H+N S +
Sbjct: 369 KLVSS-ISDNSNVTIPGGAHSNTSGI 384

BLAST of Cucsat.G3764 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 8.1e-34
Identity = 85/222 (38.29%), Postives = 130/222 (58.56%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTE-HVAPTEHT 60
           +NI +  G+AK++GT+I I GA++LT YKG       T +   H  H  T      T   
Sbjct: 127 LNIKSNVGRAKLLGTMICICGALVLTLYKG-------TALSREHSTHMETHTRTDSTGAM 186

Query: 61  IHSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQ 120
                +GS+M V S   ++ W IVQAK+S  YPC Y+ST ++   G +Q+   +L  E  
Sbjct: 187 TQKWAMGSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERS 246

Query: 121 WSRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLF 180
            S W +    ++L++ Y+GIV SG+    ++WC+R RG +F S F PLI V  AI +  F
Sbjct: 247 TSMWVVKDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSF 306

Query: 181 LQEKLYLGCVVGGMLMVCGLYMVLWGKSKE-IRKITQLAPME 221
           L E++Y G V+G M+++ GLY++LWGKSK+    +T+  P++
Sbjct: 307 LHEQIYCGSVIGSMVIIVGLYILLWGKSKDKSASVTKQEPLD 341

BLAST of Cucsat.G3764 vs. NCBI nr
Match: XP_031736965.1 (WAT1-related protein At1g25270 isoform X3 [Cucumis sativus])

HSP 1 Score: 526 bits (1354), Expect = 5.34e-187
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI
Sbjct: 71  MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 130

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 131 HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 190

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL
Sbjct: 191 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 250

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS
Sbjct: 251 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 310

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPLSKTHVTNSQHNNNSNVPHSNN
Sbjct: 311 PITPLSKTHVTNSQHNNNSNVPHSNN 336

BLAST of Cucsat.G3764 vs. NCBI nr
Match: XP_031736964.1 (WAT1-related protein At1g25270 isoform X2 [Cucumis sativus])

HSP 1 Score: 526 bits (1354), Expect = 2.04e-186
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI
Sbjct: 108 MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 167

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 168 HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 227

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL
Sbjct: 228 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 287

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS
Sbjct: 288 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 347

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPLSKTHVTNSQHNNNSNVPHSNN
Sbjct: 348 PITPLSKTHVTNSQHNNNSNVPHSNN 373

BLAST of Cucsat.G3764 vs. NCBI nr
Match: XP_004139017.2 (WAT1-related protein At1g25270 isoform X1 [Cucumis sativus] >KAE8651782.1 hypothetical protein Csa_006147 [Cucumis sativus])

HSP 1 Score: 526 bits (1354), Expect = 4.34e-186
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI
Sbjct: 129 MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 188

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 189 HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 248

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL
Sbjct: 249 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 308

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS
Sbjct: 309 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 368

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPLSKTHVTNSQHNNNSNVPHSNN
Sbjct: 369 PITPLSKTHVTNSQHNNNSNVPHSNN 394

BLAST of Cucsat.G3764 vs. NCBI nr
Match: XP_008457270.2 (PREDICTED: WAT1-related protein At1g25270-like [Cucumis melo])

HSP 1 Score: 486 bits (1252), Expect = 1.45e-170
Identity = 246/266 (92.48%), Postives = 252/266 (94.74%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGI GAMILTFYKGVELHPWSTRVDLLHK HNST H AP EH I
Sbjct: 129 MNIGTMRGKAKVMGTLIGIGGAMILTFYKGVELHPWSTRVDLLHKAHNSTGHAAPPEHKI 188

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HS VLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 189 HSQVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 248

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRL SVAYTGIVASGVMVTLITWCVR+RGPMFVSVFSPLIL+LVAIAASLFL
Sbjct: 249 SRWKLGWNIRLFSVAYTGIVASGVMVTLITWCVRMRGPMFVSVFSPLILLLVAIAASLFL 308

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLY+GCVVGGMLMVCGLYMVLWGKSKEIRKITQLAP+ESIE QLQQLG EGIDLVIS 
Sbjct: 309 QEKLYIGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPVESIEAQLQQLGSEGIDLVISP 368

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPL KTHVTN+Q +NNSN PHSNN
Sbjct: 369 PITPLPKTHVTNNQQHNNSNAPHSNN 394

BLAST of Cucsat.G3764 vs. NCBI nr
Match: KAA0060226.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 464 bits (1194), Expect = 2.67e-157
Identity = 233/249 (93.57%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGI GAMILTFYKGVELHPWSTRVDLLHK HNST H AP EH I
Sbjct: 51  MNIGTMRGKAKVMGTLIGIGGAMILTFYKGVELHPWSTRVDLLHKAHNSTGHAAPPEHKI 110

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HS VLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 111 HSQVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 170

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRL SVAYTGIVASGVMVTLITWCVR+RGPMFVSVFSPLIL+LVAIAASLFL
Sbjct: 171 SRWKLGWNIRLFSVAYTGIVASGVMVTLITWCVRMRGPMFVSVFSPLILLLVAIAASLFL 230

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLY+GCVVGGMLMVCGLYMVLWGKSKEIRKITQLAP+ESIE QLQQLG EGIDLVIS 
Sbjct: 231 QEKLYIGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPVESIEAQLQQLGSEGIDLVISP 290

Query: 241 PITPLSKTH 249
           PITPL KTH
Sbjct: 291 PITPLPKTH 299

BLAST of Cucsat.G3764 vs. ExPASy TrEMBL
Match: A0A0A0LLC4 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G151030 PE=3 SV=1)

HSP 1 Score: 526 bits (1354), Expect = 1.96e-186
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI
Sbjct: 127 MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 186

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 187 HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 246

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL
Sbjct: 247 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 306

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS
Sbjct: 307 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 366

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPLSKTHVTNSQHNNNSNVPHSNN
Sbjct: 367 PITPLSKTHVTNSQHNNNSNVPHSNN 392

BLAST of Cucsat.G3764 vs. ExPASy TrEMBL
Match: A0A1S3C5T3 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103496998 PE=3 SV=1)

HSP 1 Score: 486 bits (1252), Expect = 7.04e-171
Identity = 246/266 (92.48%), Postives = 252/266 (94.74%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGI GAMILTFYKGVELHPWSTRVDLLHK HNST H AP EH I
Sbjct: 129 MNIGTMRGKAKVMGTLIGIGGAMILTFYKGVELHPWSTRVDLLHKAHNSTGHAAPPEHKI 188

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HS VLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 189 HSQVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 248

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRL SVAYTGIVASGVMVTLITWCVR+RGPMFVSVFSPLIL+LVAIAASLFL
Sbjct: 249 SRWKLGWNIRLFSVAYTGIVASGVMVTLITWCVRMRGPMFVSVFSPLILLLVAIAASLFL 308

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLY+GCVVGGMLMVCGLYMVLWGKSKEIRKITQLAP+ESIE QLQQLG EGIDLVIS 
Sbjct: 309 QEKLYIGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPVESIEAQLQQLGSEGIDLVISP 368

Query: 241 PITPLSKTHVTNSQHNNNSNVPHSNN 266
           PITPL KTHVTN+Q +NNSN PHSNN
Sbjct: 369 PITPLPKTHVTNNQQHNNSNAPHSNN 394

BLAST of Cucsat.G3764 vs. ExPASy TrEMBL
Match: A0A5A7V174 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold386G00550 PE=3 SV=1)

HSP 1 Score: 464 bits (1194), Expect = 1.29e-157
Identity = 233/249 (93.57%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNIGTMRGKAKVMGTLIGI GAMILTFYKGVELHPWSTRVDLLHK HNST H AP EH I
Sbjct: 51  MNIGTMRGKAKVMGTLIGIGGAMILTFYKGVELHPWSTRVDLLHKAHNSTGHAAPPEHKI 110

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           HS VLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW
Sbjct: 111 HSQVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 170

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           SRWKLGWNIRL SVAYTGIVASGVMVTLITWCVR+RGPMFVSVFSPLIL+LVAIAASLFL
Sbjct: 171 SRWKLGWNIRLFSVAYTGIVASGVMVTLITWCVRMRGPMFVSVFSPLILLLVAIAASLFL 230

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           QEKLY+GCVVGGMLMVCGLYMVLWGKSKEIRKITQLAP+ESIE QLQQLG EGIDLVIS 
Sbjct: 231 QEKLYIGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPVESIEAQLQQLGSEGIDLVISP 290

Query: 241 PITPLSKTH 249
           PITPL KTH
Sbjct: 291 PITPLPKTH 299

BLAST of Cucsat.G3764 vs. ExPASy TrEMBL
Match: A0A6J1DRY7 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022969 PE=3 SV=1)

HSP 1 Score: 353 bits (905), Expect = 2.84e-118
Identity = 180/247 (72.87%), Postives = 209/247 (84.62%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           MNI TM GKAKV+GTLIGI GAM+LTFYKGVEL  WST VDLLH+ H+S  HVA  +H+I
Sbjct: 130 MNIRTMGGKAKVVGTLIGIGGAMLLTFYKGVELKLWSTHVDLLHESHHSGGHVAAPDHSI 189

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
              +LG ++GVG+C +YALWLIVQAKMSE YPCHYSSTALM +MGS QAVGFA CVE +W
Sbjct: 190 SDQLLGGLLGVGACLTYALWLIVQAKMSEYYPCHYSSTALMSLMGSFQAVGFAFCVERRW 249

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           S WKLGWNIRLLSVAY+G+VASG MVTLI+WCVR+RGP+FVS FSPLI+VLVAIA SLFL
Sbjct: 250 SEWKLGWNIRLLSVAYSGVVASGAMVTLISWCVRMRGPLFVSAFSPLIMVLVAIAGSLFL 309

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           +EKLYL C++GG+L++CGLY+VLWGKS+E++KITQL P ESIE Q      E IDLVISS
Sbjct: 310 EEKLYLECLLGGLLIICGLYIVLWGKSQEMKKITQLTPTESIEPQ-----HERIDLVISS 369

Query: 241 PITPLSK 247
           PI PL K
Sbjct: 370 PI-PLPK 370

BLAST of Cucsat.G3764 vs. ExPASy TrEMBL
Match: A0A6J1IEY2 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111472625 PE=3 SV=1)

HSP 1 Score: 345 bits (884), Expect = 3.57e-115
Identity = 182/262 (69.47%), Postives = 212/262 (80.92%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           +NI T  GKAKV+GTL+GI GAMILTFYKGVEL+ WST VDLLH   NS  HVA TEH I
Sbjct: 126 LNITTTGGKAKVVGTLLGIGGAMILTFYKGVELNLWSTHVDLLH---NSGGHVADTEHDI 185

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
            S +LGSV+G   C SY LWLIVQAKMSE YPCHYSSTALMC+M S+QAV FALC+E +W
Sbjct: 186 RSQLLGSVLGFAGCVSYGLWLIVQAKMSESYPCHYSSTALMCLMASIQAVAFALCLERRW 245

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
           S WKLGWNIRLL V+YTGIVASGV+V LI+WCVR+RGPMFVSVFSPLIL+L+AIA SLFL
Sbjct: 246 SEWKLGWNIRLLGVSYTGIVASGVIVALISWCVRMRGPMFVSVFSPLILLLLAIAGSLFL 305

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGEGIDLVISS 240
           +EKL+LGCV+GG+L++CGLY+VLWGKSKE++K TQLAPM SIE Q        IDL ISS
Sbjct: 306 EEKLHLGCVIGGVLIICGLYVVLWGKSKEMKKSTQLAPMGSIEHQPVV-----IDLPISS 365

Query: 241 PIT-PLSKTHVTNSQHNNNSNV 261
           P T P ++T      +++N NV
Sbjct: 366 PTTIPKTETDTPRGSNSSNRNV 379

BLAST of Cucsat.G3764 vs. TAIR 10
Match: AT1G25270.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-48
Identity = 97/209 (46.41%), Postives = 144/209 (68.90%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           + +G+  G+AK++GTL+G  GA++  FYKG+E+H WST VDLL   H      A T H  
Sbjct: 119 LRLGSNEGRAKLVGTLLGACGALVFVFYKGIEIHIWSTHVDLLKGSHTGR---ATTNH-- 178

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           H  +LG +M +GS  S +LWL++QAK+ +     Y +T+LM  +GS+  V  ALC +  W
Sbjct: 179 HVSILGVLMVLGSNVSTSLWLLLQAKIGKELGGLYWNTSLMNGVGSLVCVIIALCSDHDW 238

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
            +W+LGW+I LL+  Y+GIV SG++V L+ WC+  +GP+FV+VFSP+ LV+VA+  S  L
Sbjct: 239 EQWQLGWDINLLATLYSGIVVSGMVVPLVAWCIATKGPLFVTVFSPIRLVIVALIGSFAL 298

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKE 210
           +E L+LG ++G M+MV G+Y+V+W K KE
Sbjct: 299 EEPLHLGSIIGAMIMVGGVYLVVWCKMKE 322

BLAST of Cucsat.G3764 vs. TAIR 10
Match: AT1G68170.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 178.7 bits (452), Expect = 6.1e-45
Identity = 91/213 (42.72%), Postives = 141/213 (66.20%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTI 60
           + +G+  G AKV GTL G+ GA++  FY+G+E+  WST V+L+++  +S+        T 
Sbjct: 119 VRLGSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDAT----TH 178

Query: 61  HSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQW 120
           H  +LG+++  G   S +LW ++Q K+S+ +   Y +  LM MMG V A+  ALC E   
Sbjct: 179 HISILGALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDL 238

Query: 121 SRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFL 180
             W+LGWNIRLL++AY  I+ SG++V +  WC+  RGP+FVSVFSP+ LV+VA+  S  L
Sbjct: 239 DEWRLGWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLL 298

Query: 181 QEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKI 214
            E L+LG ++G +++V  LY+VLW K+KE++ +
Sbjct: 299 DETLHLGSIIGTVIIVGALYIVLWAKNKEMKSM 327

BLAST of Cucsat.G3764 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.1e-38
Identity = 88/225 (39.11%), Postives = 141/225 (62.67%), Query Frame = 0

Query: 8   GKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTEHVAPTEHTIHSHVLGS 67
           G+AKV+GTL+ ++GAM+L+FY G  +    +++   +   N T+H + + H+  +  LG 
Sbjct: 131 GQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAY-AENITKHGSSSGHS--NFFLGP 190

Query: 68  VMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQWSRWKLGW 127
            + + +  S+A W I+Q KMSE +   Y+ST LMC+MGS+Q    AL  +   S W L  
Sbjct: 191 FLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLSS 250

Query: 128 NIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLFLQEKLYLG 187
            +R +S  Y G+VAS +   L++W ++ +GP++VSVFSPL+LV+VAI +   L+EKLY G
Sbjct: 251 PLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYTG 310

Query: 188 CVVGGMLMVCGLYMVLWGKSKEIRKITQLAPMESIEEQLQQLGGE 233
             +G  L+V GLY VLWGK +E+ +  +    E +++Q  ++  E
Sbjct: 311 TFMGSALVVIGLYGVLWGKDREVSEKEE--EREKVKQQNHKVKSE 350

BLAST of Cucsat.G3764 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-37
Identity = 95/266 (35.71%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELH-PWSTRVDLLHKGH--NSTEHVAPTE 60
           +NI  +R KAK++GTL+G+ GA+++T YKG  +  PWS        GH  NS +H     
Sbjct: 129 VNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNSQDH----- 188

Query: 61  HTIHSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVE 120
              ++ V+G+++ +  C +++ + ++Q+   + YP   S +AL+C+ G+VQ+   AL VE
Sbjct: 189 ---NNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 248

Query: 121 TQWSRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAAS 180
              S W +GW+ RL +  YTGIV+SG+   +    ++ RGP+FV+ F+PL ++LVA+ AS
Sbjct: 249 RHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIAS 308

Query: 181 LFLQEKLYLGCVVGGMLMVCGLYMVLWGKSKEIRKITQLAPME--SIEEQLQQLGGEGID 240
             L E+++ GCV+GG ++  GLYMV+WGK K+  +++ L  +E  S++E       E  +
Sbjct: 309 FILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDY-EVSGLDILEKNSLQELPITTKSEDDN 368

Query: 241 LVISSPITPLSKTHVTNSQHNNNSNV 262
            ++SS I+  S   +    H+N S +
Sbjct: 369 KLVSS-ISDNSNVTIPGGAHSNTSGI 384

BLAST of Cucsat.G3764 vs. TAIR 10
Match: AT3G30340.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 145.6 bits (366), Expect = 5.7e-35
Identity = 85/222 (38.29%), Postives = 130/222 (58.56%), Query Frame = 0

Query: 1   MNIGTMRGKAKVMGTLIGIVGAMILTFYKGVELHPWSTRVDLLHKGHNSTE-HVAPTEHT 60
           +NI +  G+AK++GT+I I GA++LT YKG       T +   H  H  T      T   
Sbjct: 127 LNIKSNVGRAKLLGTMICICGALVLTLYKG-------TALSREHSTHMETHTRTDSTGAM 186

Query: 61  IHSHVLGSVMGVGSCFSYALWLIVQAKMSECYPCHYSSTALMCMMGSVQAVGFALCVETQ 120
                +GS+M V S   ++ W IVQAK+S  YPC Y+ST ++   G +Q+   +L  E  
Sbjct: 187 TQKWAMGSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERS 246

Query: 121 WSRWKLGWNIRLLSVAYTGIVASGVMVTLITWCVRIRGPMFVSVFSPLILVLVAIAASLF 180
            S W +    ++L++ Y+GIV SG+    ++WC+R RG +F S F PLI V  AI +  F
Sbjct: 247 TSMWVVKDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSF 306

Query: 181 LQEKLYLGCVVGGMLMVCGLYMVLWGKSKE-IRKITQLAPME 221
           L E++Y G V+G M+++ GLY++LWGKSK+    +T+  P++
Sbjct: 307 LHEQIYCGSVIGSMVIIVGLYILLWGKSKDKSASVTKQEPLD 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PT231.7e-4746.41WAT1-related protein At1g25270 OS=Arabidopsis thaliana OX=3702 GN=At1g25270 PE=2... [more]
F4HVM38.6e-4442.72WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3... [more]
Q8GXB41.6e-3739.11WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2... [more]
Q9SUF12.3e-3635.71WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
Q9LI658.1e-3438.29WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2... [more]
Match NameE-valueIdentityDescription
XP_031736965.15.34e-187100.00WAT1-related protein At1g25270 isoform X3 [Cucumis sativus][more]
XP_031736964.12.04e-186100.00WAT1-related protein At1g25270 isoform X2 [Cucumis sativus][more]
XP_004139017.24.34e-186100.00WAT1-related protein At1g25270 isoform X1 [Cucumis sativus] >KAE8651782.1 hypoth... [more]
XP_008457270.21.45e-17092.48PREDICTED: WAT1-related protein At1g25270-like [Cucumis melo][more]
KAA0060226.12.67e-15793.57WAT1-related protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LLC41.96e-186100.00WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_2G151030 PE=3 SV=1[more]
A0A1S3C5T37.04e-17192.48WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103496998 PE=3 SV=1[more]
A0A5A7V1741.29e-15793.57WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold386... [more]
A0A6J1DRY72.84e-11872.87WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022969 PE=3 SV=1[more]
A0A6J1IEY23.57e-11569.47WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111472625 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G25270.11.2e-4846.41nodulin MtN21 /EamA-like transporter family protein [more]
AT1G68170.16.1e-4542.72nodulin MtN21 /EamA-like transporter family protein [more]
AT1G09380.11.1e-3839.11nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.11.6e-3735.71nodulin MtN21 /EamA-like transporter family protein [more]
AT3G30340.15.7e-3538.29nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 28..103
NoneNo IPR availablePANTHERPTHR31218:SF195WAT1-RELATED PROTEINcoord: 28..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G3764.T1Cucsat.G3764.T1mRNA
Cucsat.G3764.T8Cucsat.G3764.T8mRNA
Cucsat.G3764.T4Cucsat.G3764.T4mRNA
Cucsat.G3764.T6Cucsat.G3764.T6mRNA
Cucsat.G3764.T3Cucsat.G3764.T3mRNA
Cucsat.G3764.T2Cucsat.G3764.T2mRNA
Cucsat.G3764.T5Cucsat.G3764.T5mRNA
Cucsat.G3764.T7Cucsat.G3764.T7mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity