Cucsat.G3222 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G3222
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Asparagine--tRNA ligase
Location	ctg1041: 2957702 .. 2962464 (+)
RNA-Seq Expression	Cucsat.G3222
Synteny	Cucsat.G3222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTTAGATGGAGAGTCTATTGTTTTCAAAGTTGCATTTTTTTATTATTCAAACAGTTTGTCAACAGGTCGCTTTCAAGCAAGGTTTCAATTTCCCATCAAAGAAAACAAACTCTCAGATGAAACAAACAAACTCTTCAAGAAAATCTCAACTTCAAAGTATTCCAAAACCTCAAACTCTCTTTTAAATCTCTCTCAGTTCATACCATAAAATCTCCCCAAATCACTTCTCTGAAACAATCCTTTTCCCTCCATTAACAACCTCTCTTGATTATTTCCCCCTCTACTTACCCAAGAAAAGCATCTCAATCTTCTTCTTCTTTTATATCTCTCAATCCATGTCTCTTCTCCTTCACCATTCAATCCCCCCATTTCCCTCTTTTTTCTTCCTCCACGCTCTTCCATGGCCGCCCAACAACCTTCAGTCTTCACCCCCATCACCTTCTCCAAATACTCCAACAGGGTTCTTCTAAAAACCCTCTTGGATACCACCGATGGGGGTCTTTCGTTTGTGGGTCAGAAATTTGTGGTCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATTCGAGATGCTCCCCCGGCTCCGGCTCCGGCTGATTCTCTGCCTCCTAAGGTTAATAAGGATGTGAGCTGTATTGAGATTATTCAGTCAAGGATTCCGATTGTTAGGTCTTTGCTTAAGATGTTGTATGGAAATAGTTTGCACGATGGGGAGAAGTTGGAGACTCCTGTTCATAAGCCGCCGCTTCCTTCCACTGCTTTTCTGGCTATTAATGATGGGTCTTGTGTTGCAAGCCTTCAGGTACCTCTTTTTTGTTATGCACGCAAACTCTTTGCTACTTTGTTTTGCGTATAGGATGTGACTAACTTAAAAGTTTGGCGTATTTCACTATCAAAACTTTATTATTTTGTATACAATGGTACTATTTCTTTTGTCTTTTGTCTTTTTGTTGAGAAATTATTCTGTGATCCTGCCACAGTTTTCCATCATTGTATATTCTATGACATATCTGATGGTTGTTAGAGGCATACAAATTTTGAATTCACATCCAATGGTTCTTGCGAGACAGCTACACTATTGAATAATTTGACCTTTTTATGATTAAAATGACTTTTAATGTTTTTCCCCTGTGTCTGTTTTTTAAAAAAATTGTGTCGCATATGGATGAATTTATATGTTCTTCCCTAACCAAACAAGAAATATGGAACTTTTAACAGAATTTAAAGTTGATGCAGAGTTAAGGAAGGTCTGATATTTTTTATTACAATGTGTTGGAGGATAGTCGGTTGCTTAAGCTTTGGTTGTGCTTGGTTTTTTGCATTCTCACAGTAATAAGAAACTTGTAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAGGGCGTATTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATCCTTCATGTAGGAAAGGTTGAACAGGAAAAGTACCCATTGTCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAATATGCATAAGCTTCAACCATACGTTCCATTTATTTCCTATTTTATGGTTCTGTTTGCATGCCTATAATTAGAGTGCCTATTAGCTTTAAGATCGTGCATACTGCTTATGCAACAGCACCATGTGAATTAGAACAAAGCGTTCTGTTCATATTTTATATCTAGTGATTGCCTTGAGATTCGAGAACAGGAGATTTATACAGTTAACAGCTTATTTTCTGCTAAAAGAAAGAATTGATATATATAGTTCAACGTTCCTGATCATGGATTAAAGTAACTTCATTGTAAATCTCCTTGGTAGGATCGCTTGCATTGACCTTTATAGAACCTGTGCAATGGAATTTGGTGTCAAAGCTTGAGAAGAGAGTTTTGCATATCTAATCATTAGAACTAATGAATTGTTGGATCTTGATCTTCATTGGTATTCTTTACTTTATGCAGGTAGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAACCAACAGAAGACAAAGAACCCGATGGTTTAAGCCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCCTAGTAACTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCCAAATCCAAATCAAGGAAAGATAAAGTTATTCCTGGAAATTTTTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTTTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGTATGGATTTGATCTTTTACTTATTTCTAAAGATGGCTACTTTATATTATGGGTTTAAGATTCCGTATTGATTTAGCCATTAAAATCCAATGTTATTTTCTGTGGTTAATACTTTATCATAATAACTTTGCACCATTGAAATGAAGATTTTCTATCTTTTAACTTGGATTTTTGTATTATCATTTGGAATCAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGATGATATGAAATTTGTTCAAAAACGCATTGACAAGAATAGCATCCATCGCCTCGAATCCATTATGTCATGTTCATTTGAGAAGGTCTCTTACACTACCGCAATTGAAATCCTGGAAAAGGTGAAATCTCATCTACTCTTCTATCACGTGACTCAGTTTTTCATTTCTACCCCTGCCCATCTGAATGTTGAGAAAAAAAATCTTCACTGTCAGGCAACCAATCAAAAATCTCCACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGGTGTGCAAAATGTGCTATTACAGTCGGTCACTAAGCAATTTTTCGTTACAAATAAAAAGTTCTTTCTTCTTTGTACGTTAAACAGTTATCTGGCTGATACCATCTTTAAAAAGCCTGTAATAGTGTGTAACTACCCAAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGTTAGCACATGGGCATCTATTTACCCGTGGGAAAATTTATTTTTAGATGGATCCTGAATTGAATGAAAAATTATTTCTGAAAACTGGGTCCCAACTGTTTGCAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGTATGATATCTTACTGCTTTGATTACTATTCTGTTGATATTTTTCTTGTCTATTCCATTCCAGACTTCCAAATCTTCTAAAATTGTTTTTTAATAAATTATTCAACAGAGACTTAGACATTGTCTGTCACTTCTTTTATTTATTTACTACATCAATTGATATCTACATAGGTTGGATGTTGGGGGATTCCATTATAGATTTTGGATTTTTTAAACAATTAATGAAATAAAATGGGAAGGTTGAAGATCAAGTAGGAAGATGAAATAGTTTGAGATCAAGATCAAAAGAAATTTTAACTTTCATGAATTCTTTTAAAAAAGAATACCAAAATTTTGCATTGAGGTAATGAAAAGAAACTAACGTTAGATAGGAAAAACTATAAGCAAAAATAAAAACATCAAAACTTAGAGGGATAGAAGTCCGAAAACTAAACATTCCAAAAGTGAGCTAGCCCAGGAGTAATTTGCATGATTTTTCATCTTAAAGCCTAAAGGTAAGAGGTTTTATCTTCATCCCCACAACTCAAAAAAAAAAAAAAAAAGGACTATAAATGCCATTCCAGCTGCAATCTATATTCTTGAATTGAAAGGATTTAGTTTAACTATCATGAGTTCAAACCCAGCGATAGCTGGCTTGGGATAAAATCTTACTAGTTTGACAACACTACGCTCACGGATATTAGAAAGAAAAAATGTGAGAGTTATTGATATCTGTTTGAATATTGATGGTTGTTGGCAGGATGAGGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTATTTAGAGATTCAAAGGCATGGAATAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAAAGAGGACCCAAGATGGAATTGTTCAAAAATGTTTCACAACGTTGCTGGCACCCGATCAATGGTGATGCTTGAAAAATGGAACTGAAAACTGTGTTTGTTACTGTAAGAAGTCAAGGGAAAATGACTTCTGTTACTAGAGGACTTGTGTTTGTTGCTTCCTAAGATTTGTTCTTGTAAATAAAATAGAATTTGGATTATTTGTTGAGAAGACATGATTCAAACTTGTGATCTCGAGTTCTTAATTGCTATTCTTAGCTTCAATCATAATGATTTCTTATTAAAATCTTATGCAAATCATTTTGTTTTAAATGCTTTGAT

Coding sequence (CDS)

ATGGCCGCCCAACAACCTTCAGTCTTCACCCCCATCACCTTCTCCAAATACTCCAACAGGGTTCTTCTAAAAACCCTCTTGGATACCACCGATGGGGGTCTTTCGTTTGTGGGTCAGAAATTTGTGGTCGGAGGATGGGTCAAGTCCTCCAAGGAAGTTATTCGAGATGCTCCCCCGGCTCCGGCTCCGGCTGATTCTCTGCCTCCTAAGGTTAATAAGGATGTGAGCTGTATTGAGATTATTCAGTCAAGGATTCCGATTGTTAGGTCTTTGCTTAAGATGTTGTATGGAAATAGTTTGCACGATGGGGAGAAGTTGGAGACTCCTGTTCATAAGCCGCCGCTTCCTTCCACTGCTTTTCTGGCTATTAATGATGGGTCTTGTGTTGCAAGCCTTCAGGTTGTCGTTGAGTCCTCATTACATCATCCCACCCATCTGCTGCCAACGGGAACTTGCATCTTGGTGGAGGGCGTATTGAAGCAGGTCCCGGTACCTGGGAAACATGTTGTTCGGCTTGAAGTCGAGAAAATCCTTCATGTAGGAAAGGTTGAACAGGAAAAGTACCCATTGTCCCAGAAAAAGTTGCCAATGGATTTTTTGAGGAAATTTTCTTATTTTCGTCCTCGAACAACTACGGTAGCATCTGTTACGAGAATCCGTAGTGCCTTGACTTTTGCAAATCACACGTTCTTCCAAAATAACAGCTTTCTTTACGTTCAATTACCCACCATCACAACCACAGACAGTGAGGGATTCAGCGAAAAGTTCCACGTTACAACACTTGCAGGTAAAGGTAAGAGAGTGGAACCAACAGAAGACAAAGAACCCGATGGTTTAAGCCTTGAAACTGTTAAGGCTGCTATAACGGAGAAAAGCAATCTTGTCAAAGAGCTTGAGAGAAGCGAAAGCAACAGAGAAGCCCTAGTAACTGCAGTTCAGGATTTAAAGAAAACAAATGAACTTGCATTACAATTAGAAGCCAGAGAAAAATCCAAATCCAAATCAAGGAAAGATAAAGTTATTCCTGGAAATTTTTTCCCACAAGAAACTTATCTAACGGTTTCTGGACAACTCCATTTGGAGACTTATGCATGTGCCCTTGGCAATGTTTACTCGTGTGGACCAAGGTTTCGAGCAGATAAAATTGAGTCTGCAAAACATGCAGCTGAAATGTGGATGGTGGAGGTTGAAATTGCTTTTGCTGAACTGAAGGATGCCATGAACTGTGCTGATGACCTTGTAAAATTCCTCTCCAAATGGGTGTTGGAACACTGTATGGATGATATGAAATTTGTTCAAAAACGCATTGACAAGAATAGCATCCATCGCCTCGAATCCATTATGTCATGTTCATTTGAGAAGGTCTCTTACACTACCGCAATTGAAATCCTGGAAAAGGCAACCAATCAAAAATCTCCACACTTAAAAAAGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGTTATCTGGCTGATACCATCTTTAAAAAGCCTGTAATAGTGTGTAACTACCCAAAAGAAACCAAGCCATTTAATGTCCGCCTGAATGATGATGGGAAAACCGTGGCAGCATTTGATGTGGTTCTTCCAAAGGGTGGAGTGGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTTGATGTATTGATGACAAGGATGAGGGAGATGGGTCTGCCAAGGGAGCAATACGAGTGGTATTTAGAGATTCAAAGGCATGGAATAGTGAAGCACTCTGGTTTTAGTTTGGCATTCGATCGTTTCGTTCTGTTTACTACTGGTCTCACTGACATCAAAGATGTTATTCCATTTCCAAGAAGTTTTGGAAAGGTCAGCAATTAA

Protein sequence

MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN

Homology

[Close]

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 2.3e-169
Identity = 328/626 (52.40%), Postives = 429/626 (68.53%), Query Frame = 0


Query: 11  PITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLP-- 70
           PIT SKYS RV LKTLLD +D G    G++ V+GGWVKS++ V +++PP P P  + P  
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  ------PKVNKDVSCIEIIQSRIPIVRSLLKMLYGNS----LHDGEKLETPVHKPPLPST 130
                      ++ C EIIQS++ I +    +L G      + D  +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLAINDGSCVASLQVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVE 190
            +  I+DGS ++SLQVVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199

Query: 191 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 250
           K+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + 
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259

Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEP-----DGLSLETVKAAITE 310
           F YVQ+P ITTT   GF E F VTTL GK    +  E+K+P     DG S++TVKA I E
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKE 319

Query: 311 KSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFFP 370
           K+ L+  L+RS+SNRE +V AV DLKKTN+LA Q+E ++KSK+ +  + +K+    +FF 
Sbjct: 320 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 379

Query: 371 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 430
           ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM
Sbjct: 380 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 439

Query: 431 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKA 490
           +CAD+  KFL K+VLE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+KA
Sbjct: 440 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 499

Query: 491 TNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAF 550
           T  K     K ++G ALT +HLSYL D I+K PVIV  YPK  K F VRLNDD KTVAAF
Sbjct: 500 TTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAF 559

Query: 551 DVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDR 610
           D+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL+++RHG VKHSG SL+ ++
Sbjct: 560 DLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQ 619

Query: 611 FVLFTTGLTDIKDVIPFPRSFGKVSN 614
            +L+ TGL DIKD IPFPRS+GK +N
Sbjct: 620 MLLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 2.1e-130
Identity = 256/604 (42.38%), Postives = 367/604 (60.76%), Query Frame = 0


Query: 15  SKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLPPKVNKD 74
           +++SNRVL++T+LD  DGG    GQ   +GGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  VSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAFLAINDGSCVASLQV 134
                                       G++           + +FLA+NDGSC A+LQV
Sbjct: 87  ---------------------------QGKR-----------TFSFLAVNDGSCPANLQV 146

Query: 135 VVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQEKYPLS 194
           +V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  KYPL 
Sbjct: 147 MVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYPLP 206

Query: 195 QKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEG 254
           + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT+D EG
Sbjct: 207 KTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEG 266

Query: 255 FSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV 314
             E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ +++++EA+ 
Sbjct: 267 AGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEAIT 326

Query: 315 TAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFFPQETYLTVSGQLHLETYACA 374
            AV +LK   E    ++ R + +    +KD  I    +FF ++ +LTVSGQL +ETYACA
Sbjct: 327 AAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYACA 386

Query: 375 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 434
           L NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+LE C 
Sbjct: 387 LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEKCY 446

Query: 435 DDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTD 494
            DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +       +++G  L  
Sbjct: 447 ADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDLAS 506

Query: 495 DHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER 554
           +H  YL + +F+KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER
Sbjct: 507 EHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREER 566

Query: 555 LDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPR 611
            DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R +LF TGL +I+DVIPFPR
Sbjct: 567 YDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPFPR 569

[Close]

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 4.0e-121
Identity = 249/606 (41.09%), Postives = 358/606 (59.08%), Query Frame = 0


Query: 15  SKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLPPKVNKD 74
           +++S+RVL++++L    GG    GQK  +GGWVK+ ++  +                   
Sbjct: 27  AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGKG------------------ 86

Query: 75  VSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAFLAINDGSCVASLQV 134
                                                     + AFL +NDGSC A+LQV
Sbjct: 87  ------------------------------------------TFAFLEVNDGSCPANLQV 146

Query: 135 VVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQEKYPLS 194
           +V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+   YPL 
Sbjct: 147 MVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYPLP 206

Query: 195 QKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEG 254
           + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT+D EG
Sbjct: 207 KTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEG 266

Query: 255 FSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV 314
             E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ +++++E + 
Sbjct: 267 AGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEEIT 326

Query: 315 TAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FFPQETYLTVSGQLHLETYACA 374
            +V  L         +E R + K    K+  K+   N FF ++ +LTVSGQL +ETYACA
Sbjct: 327 ASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYACA 386

Query: 375 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 434
           L +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+++ C 
Sbjct: 387 LSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDKCG 446

Query: 435 DDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQ-KSPHLKKLKFGAALT 494
           DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++G  L 
Sbjct: 447 DDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGIDLA 506

Query: 495 DDHLSYLADTIF-KKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKE 554
            +H  YL +  F +KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+E
Sbjct: 507 SEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQRE 566

Query: 555 ERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPF 611
           ER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R + F TG+ +I+DVIPF
Sbjct: 567 ERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPF 568

[Close]

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.9e-95
Identity = 198/495 (40.00%), Postives = 279/495 (56.36%), Query Frame = 0


Query: 120 FLAINDGSCVASLQVVVESSLHHPTHL---LPTGTCILVEGVLKQVPVPGKHV-VRLEVE 179
           F+ INDGS +++ Q++    +     L   L TG  +   G +  V  PGK   + ++  
Sbjct: 40  FIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTI--VESPGKEQNLEMQAT 99

Query: 180 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 239
            I  +GK + E Y L +K+   +FLR  ++ RPRT T+ +VTR+R+AL FA H FFQ   
Sbjct: 100 AITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRG 159

Query: 240 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLV 299
           FLY+  P IT +D EG  + F VTTL        P                         
Sbjct: 160 FLYIHTPIITGSDCEGAGKMFQVTTLDQNNPARTP------------------------- 219

Query: 300 KELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVS 359
                     E  V   QD                              FF + TYLTVS
Sbjct: 220 ----------EGRVDYTQD------------------------------FFGKPTYLTVS 279

Query: 360 GQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVK 419
           GQL+ E YACAL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M+CA+D +K
Sbjct: 280 GQLNGEIYACALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLK 339

Query: 420 FLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHL 479
           ++ K+VL++C +DM+F  K +  + I RLE +++ SFE+ SYT A+ ILEKA  +     
Sbjct: 340 YILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEKADKKFE--- 399

Query: 480 KKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGG 539
             +K+G  L  +H  +LA+  F KPVI+ +YPK+ K F +R N+D KTVAA DV++PK G
Sbjct: 400 YPVKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVG 459

Query: 540 VVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGL 599
            +I GSQ+EERL VL ++++E  LP E+Y WYLE+++ G V HSGF   F+R V FTTG+
Sbjct: 460 EIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGM 464

Query: 600 TDIKDVIPFPRSFGK 611
            +I+DVIPFPR  GK
Sbjct: 520 ENIRDVIPFPRHPGK 464

[Close]

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 338.2 bits (866), Expect = 2.0e-91
Identity = 198/500 (39.60%), Postives = 284/500 (56.80%), Query Frame = 0


Query: 112 KPPLPSTAFLAINDGSCVASLQVVVESSLHHPTHL---LPTGTCILVEGVLKQVPVPGK- 171
           K  L   +F+ +NDGS +A+LQVV++ SL     L   L TG  ++VEG  K  P PGK 
Sbjct: 28  KRTLKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKG 87

Query: 172 HVVRLEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFAN 231
             V L+  K+  +G  +   YPL +K+   +FLR   + RPRT T+ +V R+R+A   A 
Sbjct: 88  QRVELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAI 147

Query: 232 HTFFQNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAA 291
           H FFQ   FL+V  P IT +D EG  + F VTTL                          
Sbjct: 148 HQFFQERGFLWVHTPIITASDCEGAGDLFKVTTL-------------------------- 207

Query: 292 ITEKSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFP 351
                    +L+R   N E +     D  +                          +FF 
Sbjct: 208 ---------DLQRVPKNGEGI-----DYSQ--------------------------DFFG 267

Query: 352 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 411
           ++ YLTVSGQL  E  A A  NVY+ GP FRA+   +++H AE WMVE E+AF +L+   
Sbjct: 268 KQAYLTVSGQLEAEVMALAFQNVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDR 327

Query: 412 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKA 471
             A++ +K++ K+VLE C +DM+F  +RID   +   ++I++  F  ++Y+ AI++LEKA
Sbjct: 328 QWAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEKA 387

Query: 472 TNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAF 531
            +QK  +   + +G  L  +H  YLA+T+FK+P IV +YPK+ K F +RLNDDGKTVAA 
Sbjct: 388 -DQKFEY--PVAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDDGKTVAAM 447

Query: 532 DVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDR 591
           D++ PK G +I GSQ+EERLD+L  RM+E G+P E   WYL+++R+G V H+GF L F+R
Sbjct: 448 DILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAGFGLGFER 456

Query: 592 FVLFTTGLTDIKDVIPFPRS 608
            V F TG+ +I+DVIPFPR+
Sbjct: 508 IVQFMTGMANIRDVIPFPRT 456

[Close]

BLAST of Cucsat.G3222 vs. NCBI nr
Match: XP_004149331.1 (asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1206 bits (3121), Expect = 0.0
Identity = 611/613 (99.67%), Postives = 613/613 (100.00%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120
           PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360
           SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL
Sbjct: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480
           VLEHCMDDMKFVQKRIDKNSIHRLESI+SCSFEKVSYTTAIEILEKATNQKSPHLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480

Query: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVSN 613
           VIPFPRSFGKVSN
Sbjct: 601 VIPFPRSFGKVSN 613

[Close]

BLAST of Cucsat.G3222 vs. NCBI nr
Match: XP_008458080.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo])

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 593/613 (96.74%), Postives = 601/613 (98.04%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120
           PAPADSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360
           SESNREALV AVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHL
Sbjct: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480
           VLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEKAT+QKS HLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480

Query: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVSN 613
           VIPFPRSFGKV N
Sbjct: 601 VIPFPRSFGKVIN 613

[Close]

BLAST of Cucsat.G3222 vs. NCBI nr
Match: TYJ96325.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1160 bits (3001), Expect = 0.0
Identity = 592/617 (95.95%), Postives = 601/617 (97.41%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPA----DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
           PAPA    DSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSG 360
           ELERSESNREALV AVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLK 480
           LSKWVLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEKAT+QKS HLK
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480

Query: 481 KLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
           KLKFGAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVSN 613
           DIKDVIPFPRSFGKV N
Sbjct: 601 DIKDVIPFPRSFGKVIN 617

[Close]

BLAST of Cucsat.G3222 vs. NCBI nr
Match: KAA0026087.1 (asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1116 bits (2887), Expect = 0.0
Identity = 576/617 (93.35%), Postives = 587/617 (95.14%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPA----DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
           PAPA    DSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSG 360
           ELERSESNREALV AVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLK 480
           LSKWVLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEK  ++ S  L 
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLSTLLS 480

Query: 481 KLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
              F    T +  SYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 CDSFFLLCTLN--SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVSN 613
           DIKDVIPFPRSFGKV N
Sbjct: 601 DIKDVIPFPRSFGKVIN 615

[Close]

BLAST of Cucsat.G3222 vs. NCBI nr
Match: XP_038899280.1 (asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida])

HSP 1 Score: 1102 bits (2850), Expect = 0.0
Identity = 559/619 (90.31%), Postives = 588/619 (94.99%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAA+ P+VFTP+TFSKYSNRVLLKTLLDT DGGL+FVGQK V+GGWVKSSKEVIR+A P+
Sbjct: 1   MAAEHPTVFTPVTFSKYSNRVLLKTLLDTADGGLAFVGQKLVIGGWVKSSKEVIREAAPS 60

Query: 61  PAPA------DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPP 120
           PA A      ++LPPK  KDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKLET V KPP
Sbjct: 61  PADASVRAVANALPPKA-KDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLETAVPKPP 120

Query: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180
           LPSTAFLA+NDGSCVASLQVVVESSLHHPTHL+PTGTCILVEG+LKQVPVPGKHVVRLEV
Sbjct: 121 LPSTAFLAVNDGSCVASLQVVVESSLHHPTHLMPTGTCILVEGILKQVPVPGKHVVRLEV 180

Query: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240
           EKILHVGKVEQ+KYPLSQKKLPMDFLRKFSYFRPRTTTVASV RI +ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQQKYPLSQKKLPMDFLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNL 300
           SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGK+VEPTEDKE DG+SLETVKAAITEKSNL
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKKVEPTEDKEHDGVSLETVKAAITEKSNL 300

Query: 301 VKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTV 360
           VKELERSESN+EALV AVQDLKKTNELALQLEA+EKSK+KSRKDKVIPGNFFPQETYLT 
Sbjct: 301 VKELERSESNKEALVAAVQDLKKTNELALQLEAKEKSKTKSRKDKVIPGNFFPQETYLTA 360

Query: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLV 420
           SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAEL++AMNCADDLV
Sbjct: 361 SGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELENAMNCADDLV 420

Query: 421 KFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPH 480
           KFLSKWVL+HC+DDMKFVQKRIDK SIHRLES++SCSFEKVSY  AIEILEKAT+QKS H
Sbjct: 421 KFLSKWVLDHCLDDMKFVQKRIDKTSIHRLESVISCSFEKVSYIAAIEILEKATDQKSQH 480

Query: 481 LKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540
           LKKLKFGAALT DHLSYLAD IFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKG
Sbjct: 481 LKKLKFGAALTVDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKG 540

Query: 541 GVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTG 600
           GVVISGSQKEER DVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTG
Sbjct: 541 GVVISGSQKEERFDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTG 600

Query: 601 LTDIKDVIPFPRSFGKVSN 613
           LTDIKDVIPFPRSFGKVSN
Sbjct: 601 LTDIKDVIPFPRSFGKVSN 618

[Close]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL
Match: A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)

HSP 1 Score: 1206 bits (3121), Expect = 0.0
Identity = 611/613 (99.67%), Postives = 613/613 (100.00%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120
           PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360
           SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL
Sbjct: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480
           VLEHCMDDMKFVQKRIDKNSIHRLESI+SCSFEKVSYTTAIEILEKATNQKSPHLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480

Query: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVSN 613
           VIPFPRSFGKVSN
Sbjct: 601 VIPFPRSFGKVSN 613

[Close]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 593/613 (96.74%), Postives = 601/613 (98.04%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAF 120
           PAPADSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLPSTAF
Sbjct: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLPSTAF 120

Query: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180
           LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV
Sbjct: 121 LAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKILHV 180

Query: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240
           GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ
Sbjct: 181 GKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQ 240

Query: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVKELER 300
           LPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVKELER
Sbjct: 241 LPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVKELER 300

Query: 301 SESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSGQLHL 360
           SESNREALV AVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSGQLHL
Sbjct: 301 SESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSGQLHL 360

Query: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420
           ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW
Sbjct: 361 ETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKW 420

Query: 421 VLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKF 480
           VLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEKAT+QKS HLKKLKF
Sbjct: 421 VLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLKKLKF 480

Query: 481 GAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540
           GAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG
Sbjct: 481 GAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISG 540

Query: 541 SQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKD 600
           SQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLTDIKD
Sbjct: 541 SQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLTDIKD 600

Query: 601 VIPFPRSFGKVSN 613
           VIPFPRSFGKV N
Sbjct: 601 VIPFPRSFGKVIN 613

[Close]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 1160 bits (3001), Expect = 0.0
Identity = 592/617 (95.95%), Postives = 601/617 (97.41%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPA----DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
           PAPA    DSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSG 360
           ELERSESNREALV AVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLK 480
           LSKWVLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEKAT+QKS HLK
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKATDQKSQHLK 480

Query: 481 KLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
           KLKFGAALT DHLSYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 KLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVSN 613
           DIKDVIPFPRSFGKV N
Sbjct: 601 DIKDVIPFPRSFGKVIN 617

[Close]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL
Match: A0A5A7SMX6 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00190 PE=3 SV=1)

HSP 1 Score: 1116 bits (2887), Expect = 0.0
Identity = 576/617 (93.35%), Postives = 587/617 (95.14%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60
           MAAQQP+VF+PITFSKYSNRVLLKTLLDTTDGGLSFVGQK VVGGWVKSSKEVIRDAPPA
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  PAPA----DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLP 120
           PAPA    DSLPPK+NKDVSCIEIIQSRIPIVRSLLKMLYGN+LH GEKL+TPVHKPPLP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHKPPLP 120

Query: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180
           STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK
Sbjct: 121 STAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEK 180

Query: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240
           ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF
Sbjct: 181 ILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSF 240

Query: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEKSNLVK 300
           LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVE TEDKEPDGLSLETVKAAITEKSNLVK
Sbjct: 241 LYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSG 360
           ELERSESNREALV AVQDLKKTN+LALQLEAREKSKSKSRKDKVIPGNF PQETYLTVSG
Sbjct: 301 ELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420
           QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF
Sbjct: 361 QLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKF 420

Query: 421 LSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLK 480
           LSKWVLEHCMDDMKFVQKRIDK SIHRLESI+SCSFEKVSYT AIEILEK  ++ S  L 
Sbjct: 421 LSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILEKVKSRLSTLLS 480

Query: 481 KLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540
              F    T +  SYLADTIFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV
Sbjct: 481 CDSFFLLCTLN--SYLADTIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGV 540

Query: 541 VISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLT 600
           VISGSQKEERLDVLMTRM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVLFTTGLT
Sbjct: 541 VISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVLFTTGLT 600

Query: 601 DIKDVIPFPRSFGKVSN 613
           DIKDVIPFPRSFGKV N
Sbjct: 601 DIKDVIPFPRSFGKVIN 615

[Close]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 519/623 (83.31%), Postives = 559/623 (89.73%), Query Frame = 0


Query: 1   MAAQQPSVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAP-P 60
           MAA+ P+ FTP+TFSKYS RVLLKT+LD +DGGL FV QK V+GGWVKSSKEV R+A  P
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  APAPA----DSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPP- 120
           A A      D+LP +  KDVSCIEI+QSRIPIVRSLL+MLYGN LH GEKLETPVHKPP 
Sbjct: 61  ADASVRTAGDALPRRA-KDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPP 120

Query: 121 -LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLE 180
            LPST FL +NDGSCVASLQVVVESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLE
Sbjct: 121 PLPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLE 180

Query: 181 VEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQN 240
           VEKILHVGKVEQEKYPL+QKKLPMD LRKFSYFRPRTTTVASV RI +ALTFA HTFFQN
Sbjct: 181 VEKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQN 240

Query: 241 NSFLYVQLPTITTTDSEGFSEKFHVTTLAGKG--KRVEPTEDKEPDGLSLETVKAAITEK 300
           + FLYVQLPTITTTDSEGFSEKFHVTT AGK   K++EPTEDKE DG+SLETVKAA  EK
Sbjct: 241 HGFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEK 300

Query: 301 SNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKV-IPGNFFPQET 360
            N+VKELERSESNREAL  AVQDLKKTNELALQLEAR K KS+ +KDKV I  NFFPQET
Sbjct: 301 RNIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQET 360

Query: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCA 420
           YLTVSGQLHLETYACALGNVYSCGPRFRAD+I SAKHAAEMWMVEVEIAFAEL+D +NCA
Sbjct: 361 YLTVSGQLHLETYACALGNVYSCGPRFRADRIVSAKHAAEMWMVEVEIAFAELEDVINCA 420

Query: 421 DDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQ 480
           DDLVKFLSKWVLEHC+DDMKFVQKRIDK S+HRLES ++ SFEKVSYT A+EIL+KAT+Q
Sbjct: 421 DDLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAAVEILDKATDQ 480

Query: 481 KSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVV 540
           KS  LK+LKFGAALTDDHLSYLAD IFKKPVIV NYPKETKPFNVRLNDDGKTVAAFDVV
Sbjct: 481 KSQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVV 540

Query: 541 LPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVL 600
           LPKGGVVISGSQKEER DVL++RM+EMGLPREQYEWYLEIQRHG VKHSGFSLAFDRFVL
Sbjct: 541 LPKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQRHGTVKHSGFSLAFDRFVL 600

Query: 601 FTTGLTDIKDVIPFPRSFGKVSN 613
           FTTGLTDIKDVIPFPRS GKVS+
Sbjct: 601 FTTGLTDIKDVIPFPRSLGKVSH 622

[Close]

BLAST of Cucsat.G3222 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 597.0 bits (1538), Expect = 1.7e-170
Identity = 328/626 (52.40%), Postives = 429/626 (68.53%), Query Frame = 0


Query: 11  PITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLP-- 70
           PIT SKYS RV LKTLLD +D G    G++ V+GGWVKS++ V +++PP P P  + P  
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  ------PKVNKDVSCIEIIQSRIPIVRSLLKMLYGNS----LHDGEKLETPVHKPPLPST 130
                      ++ C EIIQS++ I +    +L G      + D  +L      PP    
Sbjct: 80  SSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEYV 139

Query: 131 AFLAINDGSCVASLQVVVESSLH--HPTHLLPTGTCILVEGVLK-QVPVPGKHVVRLEVE 190
            +  I+DGS ++SLQVVV+S+L     T L+  GTCI+ EGVL+  +    KHV+ LE E
Sbjct: 140 FYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEAE 199

Query: 191 KILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNS 250
           K+LHVG V+ EKYPLS+K+LP+  LR FS+FRPRTTTV SVTR+ SALT A+HTF Q + 
Sbjct: 200 KLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYHG 259

Query: 251 FLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEP-----DGLSLETVKAAITE 310
           F YVQ+P ITTT   GF E F VTTL GK    +  E+K+P     DG S++TVKA I E
Sbjct: 260 FQYVQVPVITTT--TGFGEMFRVTTLLGK---TDDKEEKKPPVQEKDGFSIDTVKAVIKE 319

Query: 311 KSNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKS--RKDKV-IPGNFFP 370
           K+ L+  L+RS+SNRE +V AV DLKKTN+LA Q+E ++KSK+ +  + +K+    +FF 
Sbjct: 320 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 379

Query: 371 QETYLTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAM 430
           ++TYLT SG+ HLE+YA ALG VY+ GPRF ADKI++A+H AE W VE E+AFAEL DAM
Sbjct: 380 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 439

Query: 431 NCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKA 490
           +CAD+  KFL K+VLE+  +DMKF+ KR+DK    RLE+  S S  + SYT  I +L+KA
Sbjct: 440 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 499

Query: 491 TNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAF 550
           T  K     K ++G ALT +HLSYL D I+K PVIV  YPK  K F VRLNDD KTVAAF
Sbjct: 500 TTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDDKKTVAAF 559

Query: 551 DVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDR 610
           D+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL+++RHG VKHSG SL+ ++
Sbjct: 560 DLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQ 619

Query: 611 FVLFTTGLTDIKDVIPFPRSFGKVSN 614
            +L+ TGL DIKD IPFPRS+GK +N
Sbjct: 620 MLLYATGLPDIKDAIPFPRSWGKANN 638

[Close]

BLAST of Cucsat.G3222 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 467.6 bits (1202), Expect = 1.5e-131
Identity = 256/604 (42.38%), Postives = 367/604 (60.76%), Query Frame = 0


Query: 15  SKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLPPKVNKD 74
           +++SNRVL++T+LD  DGG    GQ   +GGWVKS ++                      
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRD---------------------- 86

Query: 75  VSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAFLAINDGSCVASLQV 134
                                       G++           + +FLA+NDGSC A+LQV
Sbjct: 87  ---------------------------QGKR-----------TFSFLAVNDGSCPANLQV 146

Query: 135 VVESSLHHPTHLLPTGTCILVEGVLKQVPVPGK---HVVRLEVEKILHVGKVEQEKYPLS 194
           +V+ SL+  ++L+ TGTC+ V+GVLK VP  GK     + L V K++ VG V+  KYPL 
Sbjct: 147 MVDPSLYDVSNLVATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYPLP 206

Query: 195 QKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEG 254
           + KL ++ LR   + R RT ++++V RIR+AL FA H+FFQ +SFLY+  P ITT+D EG
Sbjct: 207 KTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEG 266

Query: 255 FSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV 314
             E F  TTL    +R+E    ++  P    +E  +  + E+ N+V EL+ +++++EA+ 
Sbjct: 267 AGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEAIT 326

Query: 315 TAVQDLKKTNELALQLEAREKSK-SKSRKDKVI--PGNFFPQETYLTVSGQLHLETYACA 374
            AV +LK   E    ++ R + +    +KD  I    +FF ++ +LTVSGQL +ETYACA
Sbjct: 327 AAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYACA 386

Query: 375 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 434
           L NVY+ GP FRA+   +++H AE WMVE EIAFA+L+D MNCA+  VK++  W+LE C 
Sbjct: 387 LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEKCY 446

Query: 435 DDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQKSPHLKKLKFGAALTD 494
            DM+ + K  D   I RL+ + S  F +++YT AIE+LE+A  +       +++G  L  
Sbjct: 447 ADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDLAS 506

Query: 495 DHLSYLADTIFKKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER 554
           +H  YL + +F+KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER
Sbjct: 507 EHERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREER 566

Query: 555 LDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPR 611
            DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R +LF TGL +I+DVIPFPR
Sbjct: 567 YDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPFPR 569

[Close]

BLAST of Cucsat.G3222 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 436.8 bits (1122), Expect = 2.9e-122
Identity = 249/606 (41.09%), Postives = 358/606 (59.08%), Query Frame = 0


Query: 15  SKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPAPAPADSLPPKVNKD 74
           +++S+RVL++++L    GG    GQK  +GGWVK+ ++  +                   
Sbjct: 27  AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGKG------------------ 86

Query: 75  VSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHKPPLPSTAFLAINDGSCVASLQV 134
                                                     + AFL +NDGSC A+LQV
Sbjct: 87  ------------------------------------------TFAFLEVNDGSCPANLQV 146

Query: 135 VVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHV---VRLEVEKILHVGKVEQEKYPLS 194
           +V+SSL+  + L+ TGTC+ V+GVLK +P  GK +   + L VE ++ VG V+   YPL 
Sbjct: 147 MVDSSLYDLSRLVATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYPLP 206

Query: 195 QKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLYVQLPTITTTDSEG 254
           + KL  +FLR   + R RT  +++V RIR+AL FA H+FFQ +SFLY+  P ITT+D EG
Sbjct: 207 KTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEG 266

Query: 255 FSEKFHVTTLAGKGKRVEP--TEDKEPDGLSLETVKAAITEKSNLVKELERSESNREALV 314
             E F VTTL    +RVE    ++  P    +E  +  + E+   V +L+ +++++E + 
Sbjct: 267 AGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEEIT 326

Query: 315 TAVQDLKKTNELALQLEAREKSKSKSRKD--KVIPGN-FFPQETYLTVSGQLHLETYACA 374
            +V  L         +E R + K    K+  K+   N FF ++ +LTVSGQL +ETYACA
Sbjct: 327 ASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYACA 386

Query: 375 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 434
           L +VY+ GP FRA+   +++H AE WMVE EIAFA++ D MNCA+  VK++ KW+++ C 
Sbjct: 387 LSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDKCG 446

Query: 435 DDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQ-KSPHLKKLKFGAALT 494
           DDM+ + K +D+    RL  +   SF++V+YT AIE LEKA  Q K     K+++G  L 
Sbjct: 447 DDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGIDLA 506

Query: 495 DDHLSYLADTIF-KKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKE 554
            +H  YL +  F +KP+IV NYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+E
Sbjct: 507 SEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQRE 566

Query: 555 ERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVLFTTGLTDIKDVIPF 611
           ER DV+  R+ EMGLP E YEWYL+++R+G VKH GF L F+R + F TG+ +I+DVIPF
Sbjct: 567 ERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPF 568

[Close]

BLAST of Cucsat.G3222 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 2.7e-88
Identity = 194/497 (39.03%), Postives = 285/497 (57.34%), Query Frame = 0


Query: 117 STAFLAINDGSCVASLQVVVESSLHHPTHL----LPTGTCILVEGVLKQVPVPG-KHVVR 176
           S  F+ INDGSC+++LQ V+ S       +    + TG  + V+G +  V   G K  V 
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI--VASQGTKQKVE 185

Query: 177 LEVEKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFF 236
           L+VEKI+ VG+ +   YP+ +K++  +FLR  ++ RPRT T  +V R+R+ L +A H FF
Sbjct: 186 LKVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFF 245

Query: 237 QNNSFLYVQLPTITTTDSEGFSEKFHVTTLAGKGKRVEPTEDKEPDGLSLETVKAAITEK 296
           Q + F++V  P IT +D EG  E+F VTTL                              
Sbjct: 246 QESGFVWVASPIITASDCEGAGEQFCVTTLI----------------------------- 305

Query: 297 SNLVKELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETY 356
                      S+ E   T++  + KT    +                    +FF +  +
Sbjct: 306 ----------PSSHENTDTSIDAIPKTKGGLIDWSQ----------------DFFGKPAF 365

Query: 357 LTVSGQLHLETYACALGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCAD 416
           LTVSGQL+ ETYA AL +VY+ GP FRA+   +++H AE WM+E E+AFA+L D M CA 
Sbjct: 366 LTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACAT 425

Query: 417 DLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIMSCSFEKVSYTTAIEILEKATNQK 476
             ++++ K+VL++C +DM+F    I+K  I RL  +    F ++ YT AIEIL KA N+K
Sbjct: 426 AYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKA-NKK 485

Query: 477 SPHLKKLKFGAALTDDHLSYLADTIF-KKPVIVCNYPKETKPFNVRLNDDGKTVAAFDVV 536
                 +K+G  L  +H  Y+ +  F  +PVI+ +YPKE K F +R NDDGKTVAA D++
Sbjct: 486 FDF--PVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDML 545

Query: 537 LPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVKHSGFSLAFDRFVL 596
           +P+ G +I GSQ+EERL+VL  R+ E+ L +E Y WYL+++R+G V H+GF L F+R V 
Sbjct: 546 VPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQ 561

Query: 597 FTTGLTDIKDVIPFPRS 608
           F TG+ +I+DVIPFPR+
Sbjct: 606 FVTGIDNIRDVIPFPRT 561

[Close]

BLAST of Cucsat.G3222 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-15
Identity = 35/77 (45.45%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 367 LGNVYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKDAMNCADDLVKFLSKWVLEHCM 426
           +G+VY+  P FRA+K  +++H AE WMVEVE+AFA +++AMNC++ +VK +   +LE C 
Sbjct: 1   MGDVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCR 60

Query: 427 DDMKFVQKRIDKNSIHR 444
           DDM+++ +++D+  I R
Sbjct: 61  DDMEYMVEKVDEFCIDR 77

The following BLAST results are available for this feature:

BLAST of Cucsat.G3222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW95	2.3e-169	52.40	Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ...	[more]
Q9SW96	2.1e-130	42.38	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	4.0e-121	41.09	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	2.9e-95	40.00	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
P52276	2.0e-91	39.60	Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117...	[more]

BLAST of Cucsat.G3222 vs. NCBI nr


Match Name	Identity	Description
XP_004149331.1	99.67	asparagine--tRNA ligase, cytoplasmic 2 isoform X1 [Cucumis sativus]	[more]
XP_008458080.1	96.74	PREDICTED: asparagine--tRNA ligase, cytoplasmic 2 [Cucumis melo]	[more]
TYJ96325.1	95.95	asparagine--tRNA ligase [Cucumis melo var. makuwa]	[more]
KAA0026087.1	93.35	asparagine--tRNA ligase [Cucumis melo var. makuwa]	[more]
XP_038899280.1	90.31	asparagine--tRNA ligase, cytoplasmic 2 [Benincasa hispida]	[more]

BLAST of Cucsat.G3222 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0K8G1	99.67	Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1	[more]
A0A1S3C6Z5	96.74	Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1	[more]
A0A5D3BD07	95.95	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5A7SMX6	93.35	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A6J1KTV9	83.31	asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of Cucsat.G3222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07420.1	1.7e-170	52.40	asparaginyl-tRNA synthetase 2	[more]
AT5G56680.1	1.5e-131	42.38	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	2.9e-122	41.09	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	2.7e-88	39.03	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G68420.1	1.8e-15	45.45	Class II aaRS and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 85..122
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 12..416 e-value: 3.2E-81 score: 275.3
None	No IPR available	PANTHER	PTHR22594:SF36	ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATED	coord: 17..417
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 17..417
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 18..411
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 372..386 score: 32.58 coord: 172..185 score: 32.47 coord: 156..168 score: 41.78 coord: 332..348 score: 27.94
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 17..415 e-value: 2.9E-105 score: 350.6
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 17..64 e-value: 1.8E-4 score: 20.7
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 142..410 e-value: 2.3E-52 score: 178.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G3222.T1	Cucsat.G3222.T1	mRNA
Cucsat.G3222.T2	Cucsat.G3222.T2	mRNA
Cucsat.G3222.T3	Cucsat.G3222.T3	mRNA
Cucsat.G3222.T4	Cucsat.G3222.T4	mRNA
Cucsat.G3222.T7	Cucsat.G3222.T7	mRNA
Cucsat.G3222.T8	Cucsat.G3222.T8	mRNA
Cucsat.G3222.T5	Cucsat.G3222.T5	mRNA
Cucsat.G3222.T6	Cucsat.G3222.T6	mRNA
Cucsat.G3222.T9	Cucsat.G3222.T9	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding