Cucsat.G322 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G322
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	WRKY transcription factor 22
Location	ctg1: 7129178 .. 7131224 (-)
RNA-Seq Expression	Cucsat.G322
Synteny	Cucsat.G322

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATTTTGGTTTCTATATATAATTCTTTTCCTACTCATTACTCATTACACCCTTATCACCAAAAGTCAGCGCCTTCTTTTGTCTCTATTTTTCTCTCTTAGAACAAAACAACCAAAAAAGAAGAAAGAAAAAAAAGAAACTGATCAAAGAGAAGAAGTTGAGAGAGACAAAGGTACAGATTCTCAGAGCAGAGTTCTGTCTTTTGATAATGGAAGTCGATTGGGATCTTCATGCCGTCGTCAGAGGTTACTCCGCCGCGCCCTCTGCTGCTACCATTGTTCCCGCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAATAACCCTGTCGTCCCTTTTTCTTTTGGTAGAGACCTAACCAACAATCAAATGAAAAACCATTTCTTCTCTCTTCAAGATCCATTTCAACCCTCTAATTGTAATTCCACTCAAGAATTGCATGAGCTTTTCAAACCCTTTTTCCCTAAATCTCAACCATCTCCTTCTCCTCCGCCGCCTCCTCCTGCACCGCCTGCTCCGTCGCTTCTTTCTTCTCCTCCGGCTCCTAAAATTTTGACTCATCAGAAACAGAGCACCCATCTCCCTAAACAACTCCATTCAACCTCTGCTTCTGCTCCGAGATCCAAACGCCGGTGAGCAAGATTCTTCCATTTCTTCTTCATTTTGGATGTGTGTTTGTTTGTTTGTTTGTATTTCTTTTAGCTAATCGATCTTTTGTTTTGGTTCTCTTTTTAGGAAGAATCAGCTGAAGAAGGTTTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGTTTGTGCTCAATTTCAATTCTGAGATTAGGGTTTTTTAGGGTTTTGATTTTGGGGTTAGAACTAATTTTTGGTGCTTTTTTAAAAAATAACAGGGGATATTATAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTGGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCTACTCACCGCAACTCTCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCGTCCACCACCGCGTCCGGATCTGAAAAACTCGACCCGAAACAACCGGTCTGCTCGTCGGAAGAACAAAGTACGATCACGGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGAATCTCCGATTTGATCGTGAACGACGATTTCTATGTGGGTTTTGAAGAACTTGACAGCCCAATAACCGACGACTGCTTTTCCGATCCATTCCCGGCCAACTTCGACCTTCCATGGTTGTTTAACGGAAATCCAGACGGCGAAATTGTAAAACTCCCTTGTTAAATTAATTTCCAACTTTTTTTTTTTATAATCATTCTCCAAACAGAGATCTGTAAAATCAAAGATGTTAGAGCAAAGATTTTAACAGTGTTTTTAGAAAGAGAAAGAGCGAGCAAGATAGTAGTAAATTTCGTCTTGTTTTCTCTGTAATTTTAGAAGAACAGTGATATTGAAGTTGAAGATGCAGTGGGTTTTTGTGAGAATTTTTTTAGGGAAAAAGGAATGAACAACATAAGGGGGGGTTGACTAATTAAAAGAAAAGAAGTGGAATCAAGCAATGAATGAATGACGTGTAGATGGCGTTGACAGAGATGGAAAATTAGAAGAGAGATTAAAAGCAGAGGTTGAAGAAGAGAAAATGGGTATGGAGGAGGAAGATTAAGAATATTGGCTTCTTTTTTCCAAAATGCTTGTCCGAGGACAACAATGGTGGTGGTGGTGATGGTGGAGAATCAGATTTTTTCTTTTGTTTATAGATGAAGAAAAAAAGAAAAAAAAAGAAAAAAAAGAGGATTTGGAATTTTTCATTGTAGTTGATGCCAGTTGTGGTCATAGTGGTAAAGATAAATGGTAGAGATTCAATATCAATATTGTGTATAATTGAATCCAAAATTTTCATTTGTGTTTTTTTCT

Coding sequence (CDS)

ATGGAAGTCGATTGGGATCTTCATGCCGTCGTCAGAGGTTACTCCGCCGCGCCCTCTGCTGCTACCATTGTTCCCGCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAATAACCCTGTCGTCCCTTTTTCTTTTGGTAGAGACCTAACCAACAATCAAATGAAAAACCATTTCTTCTCTCTTCAAGATCCATTTCAACCCTCTAATTGTAATTCCACTCAAGAATTGCATGAGCTTTTCAAACCCTTTTTCCCTAAATCTCAACCATCTCCTTCTCCTCCGCCGCCTCCTCCTGCACCGCCTGCTCCGTCGCTTCTTTCTTCTCCTCCGGCTCCTAAAATTTTGACTCATCAGAAACAGAGCACCCATCTCCCTAAACAACTCCATTCAACCTCTGCTTCTGCTCCGAGATCCAAACGCCGGAAGAATCAGCTGAAGAAGGTTTGTCAAGTTCCGGCGGAGTCTCTGTCTTCGGATATTTGGGCTTGGCGAAAATATGGACAAAAACCCATTAAAGGATCTCCATATCCAAGGGGATATTATAGATGTAGCAGTTCGAAGGGTTGCATGGCCCGAAAACAAGTGGAGAGGAACAGATCCGACCCGGGAATGTTCATAGTCACTTACACGGCGGAGCACAACCACCCGGCGCCTACTCACCGCAACTCTCTCGCCGGCTCCACCCGTCAGAAACCCATCACACCGTCCACCACCGCGTCCGGATCTGAAAAACTCGACCCGAAACAACCGGTCTGCTCGTCGGAAGAACAAAGTACGATCACGGAGAGCAAAGAAGAGAAAGAGGAATTATTAATGGCCGAAGATGAAGAAGACGACGATCTGGGAATCTCCGATTTGATCGTGAACGACGATTTCTATGTGGGTTTTGAAGAACTTGACAGCCCAATAACCGACGACTGCTTTTCCGATCCATTCCCGGCCAACTTCGACCTTCCATGGTTGTTTAACGGAAATCCAGACGGCGAAATTGTAAAACTCCCTTGTTAA

Protein sequence

MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC

Homology

[Close]

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 157/332 (47.29%), Postives = 194/332 (58.43%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           M  DWDLHAVVRG SA  S+AT    ++  S   SS  NP+  F+ GR            
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT----TTVYSPGVSSHTNPI--FTVGRQ----------- 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
                   SN  S  E+ +L+ PF  +S  S           + S ++ P  P+   +QK
Sbjct: 61  --------SNAVSFGEIRDLYTPFTQESVVS-----------SFSCINYPEEPRKPQNQK 120

Query: 121 QSTHLPKQLHS-----TSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGS 180
           +   L     S     + ++  RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGS
Sbjct: 121 RPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGS 180

Query: 181 PYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPIT 240
           PYPRGYYRCS+SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP  
Sbjct: 181 PYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSD 240

Query: 241 PSTTASGSEKLD--PKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDF 300
             T+ S +  +      PV S++E     E      +L    D E+D L +SD +V+DDF
Sbjct: 241 QQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDF 289

Query: 301 YVGFEELDSPITDDCFS-DPFPANFDLPWLFN 325
           + G EE       D FS +  PA+FDL W+ N
Sbjct: 301 FDGLEEF---AAGDSFSGNSAPASFDLSWVVN 289

[Close]

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.6e-39
Identity = 131/338 (38.76%), Postives = 167/338 (49.41%), Query Frame = 0


Query: 4   DWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQ 63
           DWDL AVVR  S++ S             +     +P  P  F                 
Sbjct: 5   DWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQQDPPPPPLFQAS-------------- 64

Query: 64  DPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ-- 123
                S+CN   EL +  KPF P +  + +   PPP  P P   +S P+P IL  Q+Q  
Sbjct: 65  -----SSCN---ELQDSCKPFLPVTTTTTTTWSPPPLLPPPK--ASSPSPNILLKQEQVL 124

Query: 124 -----------------ST---------HLPKQLHSTSASAPRSKRRKNQLKK-VCQVPA 183
                            ST            + L   S    RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITP---------STTASGSEKLDPKQPVCSSEE------ 290
            HP PTHRNSLAGSTR K  P+ P         S T      L P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

[Close]

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 7.8e-34
Identity = 79/150 (52.67%), Postives = 102/150 (68.00%), Query Frame = 0


Query: 134 ASAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 193
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSPYPRG
Sbjct: 177 SSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRG 236

Query: 194 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 253
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+LAGSTR      S+++
Sbjct: 237 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRS-----SSSS 296

Query: 254 SGSEKLDPKQPVCSSEEQSTITESKEEKEE 271
           S +          ++   S + ++   K+E
Sbjct: 297 SLNPSSKSSTAAATTSPSSRVFQNNSSKDE 321

[Close]

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.6e-29
Identity = 108/275 (39.27%), Postives = 142/275 (51.64%), Query Frame = 0


Query: 6   DLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDP 65
           DL A+VRGYS +  A     +  SS + S S   P+   SF                   
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETSSF------------------- 64

Query: 66  FQPS-NCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ 125
           ++P    +   EL EL+KPF+P S     + S   P  + P            +L++  +
Sbjct: 65  YEPEMETSGLDELGELYKPFYPFSTQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSR 124

Query: 126 STHLPKQLHSTSASAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRG 185
           + H+        + +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR 
Sbjct: 125 ADHI------RISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRS 184

Query: 186 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 245
           YYRCSSSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P  T 
Sbjct: 185 YYRCSSSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTL 244

Query: 246 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAE 276
           +   + +      S+    +  ES    +E+ +AE
Sbjct: 245 TKKSEKEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

[Close]

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 6.4e-28
Identity = 109/300 (36.33%), Postives = 149/300 (49.67%), Query Frame = 0


Query: 44  FSFGRDLTNNQMKNHFFSLQDPFQ----PSNCNSTQELHELFKPFFPKSQPSPSPPPPPP 103
           FS   D  NN   N+ F +   F+     S C+    +          S P  SP     
Sbjct: 82  FSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAH 141

Query: 104 APPAPSLLSSPPAPKILTHQKQS------THLPKQLHSTSASAPRSKRRKNQLKKVCQVP 163
              A +  S      + T+  ++      T    Q+  +S      KRRK+Q KKV  +P
Sbjct: 142 VVAAAAAASPRGIINVDTNSPRNCLLVDGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIP 201

Query: 164 A----------ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDP 223
           A          E + SD+WAWRKYGQKPIKGSP+PRGYYRCSSSKGC ARKQVER+R+DP
Sbjct: 202 APAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDP 261

Query: 224 GMFIVTYTAEHNHPAPTHRNSLAGSTRQ--------KPITPSTTASGSEKLDPKQPV--- 283
            M ++TYT+EHNHP P  RN+LAGSTR          P  PST    S  +  +  +   
Sbjct: 262 NMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLP 321

Query: 284 CSSEEQSTITES--KEEK-EELLMAEDEEDDDLGISDLIVN--------DDFYVGFEELD 302
            S+    T++ S  K+E+ +++ +   ++DDD  I+             DDF+   EEL+
Sbjct: 322 SSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELE 381

[Close]

BLAST of Cucsat.G322 vs. NCBI nr
Match: NP_001267594.1 (WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cucumis sativus] >KAE8650243.1 hypothetical protein Csa_011009 [Cucumis sativus])

HSP 1 Score: 662 bits (1708), Expect = 1.02e-239
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

[Close]

BLAST of Cucsat.G322 vs. NCBI nr
Match: TYK17626.1 (WRKY transcription factor 22 [Cucumis melo var. makuwa])

HSP 1 Score: 609 bits (1570), Expect = 9.49e-219
Identity = 314/339 (92.63%), Postives = 321/339 (94.69%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSSS S++S      VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSFSNNS------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPS---PSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPS   P+PPPPPP P AP LLSSP APKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSP-APKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

[Close]

BLAST of Cucsat.G322 vs. NCBI nr
Match: XP_008437986.1 (PREDICTED: WRKY transcription factor 22 [Cucumis melo])

HSP 1 Score: 607 bits (1566), Expect = 3.72e-218
Identity = 313/339 (92.33%), Postives = 320/339 (94.40%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSS S++S       VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSFSNNS-------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPS---PSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPS   P+PPPPPP P AP LLSSP APKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSP-APKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

[Close]

BLAST of Cucsat.G322 vs. NCBI nr
Match: XP_038880705.1 (WRKY transcription factor 22 [Benincasa hispida])

HSP 1 Score: 577 bits (1488), Expect = 2.46e-206
Identity = 303/338 (89.64%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  S ATI+P+SSSSSS++S      +PFSF RDLTN Q KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAVSSTATIIPSSSSSSSNNS------IPFSFSRDLTN-QTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILT--H 120
           SLQDPFQP NCNSTQELHELFKPFFPKSQPSP PPPPPPAPP   LLSSP A KILT   
Sbjct: 61  SLQDPFQPPNCNSTQELHELFKPFFPKSQPSPPPPPPPPAPP---LLSSP-AAKILTPPK 120

Query: 121 QKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
           Q+QSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 121 QQQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITP+ 
Sbjct: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPTP 240

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
           TASGS K DPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGFE
Sbjct: 241 TASGSGKPDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGFE 300

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           ELDSPITDDCFSDPFPANFDLPWLFNGNPD EIVKLPC
Sbjct: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDSEIVKLPC 327

[Close]

BLAST of Cucsat.G322 vs. NCBI nr
Match: XP_023528010.1 (WRKY transcription factor 22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511 bits (1315), Expect = 5.89e-180
Identity = 278/337 (82.49%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  SAATI P+S   S SS+S+N+   PF+FGRDLTN    NHFF
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATI-PSSDFYSVSSTSNNS--TPFAFGRDLTN--QTNHFF 62

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPF+  NCNST+ELHELFKPF PK  P PSPPPPPP+  AP LLSSP A KILTHQK
Sbjct: 63  SLQDPFEGPNCNSTEELHELFKPFSPK--PLPSPPPPPPS--APLLLSSP-AAKILTHQK 122

Query: 121 Q--STHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
           Q  +TH+PKQLHS   SA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 123 QRQNTHVPKQLHSAPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 182

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           R YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T + 
Sbjct: 183 RXYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTP 242

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
            +SGSEK DPKQPVCSSE+QSTITESKEEKEELL AEDEEDDDLG+SDLIVNDDFY+GFE
Sbjct: 243 ASSGSEKPDPKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFE 302

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLP 335
           ELDSPITD+CFSD FPANF+LPW FNG+PD EIVKLP
Sbjct: 303 ELDSPITDECFSDQFPANFELPWSFNGDPDSEIVKLP 328

[Close]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL
Match: E7CEW7 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1)

HSP 1 Score: 662 bits (1708), Expect = 4.94e-240
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336

[Close]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL
Match: A0A0A0L7C1 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 9.21e-238
Identity = 335/336 (99.70%), Postives = 335/336 (99.70%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSS NNPVVPFSFGRDLTNNQMKNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSS-NNPVVPFSFGRDLTNNQMKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120

Query: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180
           QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG
Sbjct: 121 QSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYPRG 180

Query: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240
           YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA
Sbjct: 181 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 240

Query: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300
           SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL
Sbjct: 241 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFEEL 300

Query: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 DSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 335

[Close]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL
Match: A0A5D3D3F9 (WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004970 PE=4 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 4.59e-219
Identity = 314/339 (92.63%), Postives = 321/339 (94.69%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSSS S++S      VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSSFSNNS------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPS---PSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPS   P+PPPPPP P AP LLSSP APKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSP-APKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 332

[Close]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL
Match: A0A1S3AVV5 (WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1)

HSP 1 Score: 607 bits (1566), Expect = 1.80e-218
Identity = 313/339 (92.33%), Postives = 320/339 (94.40%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSAAPSAATIVP+SSS S++S       VPFSFGRDLTNNQ KNHFF
Sbjct: 1   MEVDWDLHAVVRGYSAAPSAATIVPSSSSFSNNS-------VPFSFGRDLTNNQTKNHFF 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPS---PSPPPPPPAPPAPSLLSSPPAPKILT 120
           SLQDPFQPSNCNSTQELHELFKPFFPKSQPS   P+PPPPPP P AP LLSSP APKILT
Sbjct: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSSSSPTPPPPPPPPAAPPLLSSP-APKILT 120

Query: 121 HQKQSTHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180
           H KQSTHLPKQLHSTS SAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY
Sbjct: 121 HHKQSTHLPKQLHSTSVSAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPY 180

Query: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPS 240
           PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP+TP+
Sbjct: 181 PRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPVTPT 240

Query: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGF 300
           TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLG+SDLIVNDDFYVGF
Sbjct: 241 TTASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGVSDLIVNDDFYVGF 300

Query: 301 EELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLPC 336
           EELDSPITDDCFSD FPANFDLPWLFNGNPDGEIVKLPC
Sbjct: 301 EELDSPITDDCFSDQFPANFDLPWLFNGNPDGEIVKLPC 331

[Close]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL
Match: A0A6J1EEC7 (WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 4.05e-180
Identity = 278/337 (82.49%), Postives = 296/337 (87.83%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           MEVDWDLHAVVRGYSA  SAATI P+S   S SS+S+N+   PF+FGRDLTN    NHFF
Sbjct: 3   MEVDWDLHAVVRGYSAVSSAATI-PSSDFYSVSSTSNNS--TPFAFGRDLTN--QTNHFF 62

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
           SLQDPF+  NCNST+ELHELFKPF PK  P PSPPPPPP+  AP LLSSP A KILTHQK
Sbjct: 63  SLQDPFEGPNCNSTEELHELFKPFSPK--PLPSPPPPPPS--APLLLSSP-AAKILTHQK 122

Query: 121 Q--STHLPKQLHSTSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 180
           Q  +TH+PKQLHS   SA RSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP
Sbjct: 123 QRQNTHVPKQLHSAPVSASRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGSPYP 182

Query: 181 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPST 240
           RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKP T + 
Sbjct: 183 RGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPNTQTP 242

Query: 241 TASGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDFYVGFE 300
            +SGSEK D KQPVCSSE+QSTITESKEEKEELL AEDEEDDDLG+SDLIVNDDFY+GFE
Sbjct: 243 ASSGSEKPDSKQPVCSSEDQSTITESKEEKEELL-AEDEEDDDLGVSDLIVNDDFYMGFE 302

Query: 301 ELDSPITDDCFSDPFPANFDLPWLFNGNPDGEIVKLP 335
           ELDSPITD+CFSD FPANF+LPW FNG+PD EIVKLP
Sbjct: 303 ELDSPITDECFSDQFPANFELPWSFNGDPDSEIVKLP 328

[Close]

BLAST of Cucsat.G322 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 221.9 bits (564), Expect = 8.0e-58
Identity = 157/332 (47.29%), Postives = 194/332 (58.43%), Query Frame = 0


Query: 1   MEVDWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFF 60
           M  DWDLHAVVRG SA  S+AT    ++  S   SS  NP+  F+ GR            
Sbjct: 1   MADDWDLHAVVRGCSAVSSSAT----TTVYSPGVSSHTNPI--FTVGRQ----------- 60

Query: 61  SLQDPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQK 120
                   SN  S  E+ +L+ PF  +S  S           + S ++ P  P+   +QK
Sbjct: 61  --------SNAVSFGEIRDLYTPFTQESVVS-----------SFSCINYPEEPRKPQNQK 120

Query: 121 QSTHLPKQLHS-----TSASAPRSKRRKNQLKKVCQVPAESLSSDIWAWRKYGQKPIKGS 180
           +   L     S     + ++  RSKRRK Q KKVC V AE+L+SD+WAWRKYGQKPIKGS
Sbjct: 121 RPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAAEALNSDVWAWRKYGQKPIKGS 180

Query: 181 PYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPIT 240
           PYPRGYYRCS+SKGC+ARKQVERNRSDP MFIVTYTAEHNHPAPTHRNSLAGSTRQKP  
Sbjct: 181 PYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSD 240

Query: 241 PSTTASGSEKLD--PKQPVCSSEEQSTITESKEEKEELLMAEDEEDDDLGISDLIVNDDF 300
             T+ S +  +      PV S++E     E      +L    D E+D L +SD +V+DDF
Sbjct: 241 QQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGDL----DGEEDLLSLSDTVVSDDF 289

Query: 301 YVGFEELDSPITDDCFS-DPFPANFDLPWLFN 325
           + G EE       D FS +  PA+FDL W+ N
Sbjct: 301 FDGLEEF---AAGDSFSGNSAPASFDLSWVVN 289

[Close]

BLAST of Cucsat.G322 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 163.7 bits (413), Expect = 2.6e-40
Identity = 131/338 (38.76%), Postives = 167/338 (49.41%), Query Frame = 0


Query: 4   DWDLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQ 63
           DWDL AVVR  S++ S             +     +P  P  F                 
Sbjct: 5   DWDLFAVVRSCSSSVSTTNSCAGHEDDIGNCKQQQDPPPPPLFQAS-------------- 64

Query: 64  DPFQPSNCNSTQELHELFKPFFPKSQPSPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ-- 123
                S+CN   EL +  KPF P +  + +   PPP  P P   +S P+P IL  Q+Q  
Sbjct: 65  -----SSCN---ELQDSCKPFLPVTTTTTTTWSPPPLLPPPK--ASSPSPNILLKQEQVL 124

Query: 124 -----------------ST---------HLPKQLHSTSASAPRSKRRKNQLKK-VCQVPA 183
                            ST            + L   S    RS++RKNQ K+ +C V  
Sbjct: 125 LESQDQKPPLSVRVFPPSTSSSVFVFRGQRDQLLQQQSQPPLRSRKRKNQQKRTICHVTQ 184

Query: 184 ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEH 243
           E+LSSD+WAWRKYGQKPIKGSPYPR YYRCSSSKGC+ARKQVER+  DP +FIVTYT EH
Sbjct: 185 ENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQVERSNLDPNIFIVTYTGEH 244

Query: 244 NHPAPTHRNSLAGSTRQK--PITP---------STTASGSEKLDPKQPVCSSEE------ 290
            HP PTHRNSLAGSTR K  P+ P         S T      L P  P+  +++      
Sbjct: 245 THPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLSDTVKEEIHLSPTTPLKGNDDVQETNG 304

[Close]

BLAST of Cucsat.G322 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 146.0 bits (367), Expect = 5.5e-35
Identity = 79/150 (52.67%), Postives = 102/150 (68.00%), Query Frame = 0


Query: 134 ASAPRS---KRRKNQLKKVCQVPA----------ESLSSDIWAWRKYGQKPIKGSPYPRG 193
           +S+PR+   KRRK+Q KKV  +PA          E + SD+WAWRKYGQKPIKGSPYPRG
Sbjct: 177 SSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPYPRG 236

Query: 194 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 253
           YYRCSSSKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+LAGSTR      S+++
Sbjct: 237 YYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRS-----SSSS 296

Query: 254 SGSEKLDPKQPVCSSEEQSTITESKEEKEE 271
           S +          ++   S + ++   K+E
Sbjct: 297 SLNPSSKSSTAAATTSPSSRVFQNNSSKDE 321

[Close]

BLAST of Cucsat.G322 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 129.4 bits (324), Expect = 5.4e-30
Identity = 108/275 (39.27%), Postives = 142/275 (51.64%), Query Frame = 0


Query: 6   DLHAVVRGYSAAPSAATIVPASSSSSSSSSSSNNPVVPFSFGRDLTNNQMKNHFFSLQDP 65
           DL A+VRGYS +  A     +  SS + S S   P+   SF                   
Sbjct: 5   DLEAIVRGYSGSGDAF----SGESSGTFSPSFCLPMETSSF------------------- 64

Query: 66  FQPS-NCNSTQELHELFKPFFPKSQP---SPSPPPPPPAPPAPSLLSSPPAPKILTHQKQ 125
           ++P    +   EL EL+KPF+P S     + S   P  + P            +L++  +
Sbjct: 65  YEPEMETSGLDELGELYKPFYPFSTQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSR 124

Query: 126 STHLPKQLHSTSASAPRSKRRKNQLKKVC-QVPAESLSSDIWAWRKYGQKPIKGSPYPRG 185
           + H+        + +   K +KNQ K+V  QV  E+L SD WAWRKYGQKPIKGSPYPR 
Sbjct: 125 ADHI------RISESKSKKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRS 184

Query: 186 YYRCSSSKGCMARKQVERNRSDPGMFIVTYTAEHNHPAPTHRNSLAGSTRQKPITPSTTA 245
           YYRCSSSKGC+ARKQVERN  +P  F +TYT EHNH  PT RNSLAGSTR K   P  T 
Sbjct: 185 YYRCSSSKGCLARKQVERNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTL 244

Query: 246 SGSEKLDPKQPVCSSEEQSTITESKEEKEELLMAE 276
           +   + +      S+    +  ES    +E+ +AE
Sbjct: 245 TKKSEKEVVSSPTSNPMIPSADESSVAVQEMSVAE 250

[Close]

BLAST of Cucsat.G322 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-29
Identity = 109/300 (36.33%), Postives = 149/300 (49.67%), Query Frame = 0


Query: 44  FSFGRDLTNNQMKNHFFSLQDPFQ----PSNCNSTQELHELFKPFFPKSQPSPSPPPPPP 103
           FS   D  NN   N+ F +   F+     S C+    +          S P  SP     
Sbjct: 82  FSTVGDNNNNIHNNNGFLVPKVFEEDHIKSQCSIFPRIRISHSNIIHDSSPCNSPAMSAH 141

Query: 104 APPAPSLLSSPPAPKILTHQKQS------THLPKQLHSTSASAPRSKRRKNQLKKVCQVP 163
              A +  S      + T+  ++      T    Q+  +S      KRRK+Q KKV  +P
Sbjct: 142 VVAAAAAASPRGIINVDTNSPRNCLLVDGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIP 201

Query: 164 A----------ESLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCMARKQVERNRSDP 223
           A          E + SD+WAWRKYGQKPIKGSP+PRGYYRCSSSKGC ARKQVER+R+DP
Sbjct: 202 APAAMNSRSSGEVVPSDLWAWRKYGQKPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDP 261

Query: 224 GMFIVTYTAEHNHPAPTHRNSLAGSTRQ--------KPITPSTTASGSEKLDPKQPV--- 283
            M ++TYT+EHNHP P  RN+LAGSTR          P  PST    S  +  +  +   
Sbjct: 262 NMLVITYTSEHNHPWPIQRNALAGSTRSSTSSSSNPNPSKPSTANVNSSSIGSQNTIYLP 321

Query: 284 CSSEEQSTITES--KEEK-EELLMAEDEEDDDLGISDLIVN--------DDFYVGFEELD 302
            S+    T++ S  K+E+ +++ +   ++DDD  I+             DDF+   EEL+
Sbjct: 322 SSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRPELHDHQHQPDDFFADLEELE 381

The following BLAST results are available for this feature:

BLAST of Cucsat.G322 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04609	1.1e-56	47.29	WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1	[more]
Q9FLX8	3.6e-39	38.76	Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ...	[more]
O64747	7.8e-34	52.67	Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ...	[more]
Q9SUS1	7.6e-29	39.27	Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ...	[more]
Q9SA80	6.4e-28	36.33	Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ...	[more]

BLAST of Cucsat.G322 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267594.1	1.02e-239	100.00	WRKY transcription factor 22-like [Cucumis sativus] >ADU52510.1 WRKY protein [Cu...	[more]
TYK17626.1	9.49e-219	92.63	WRKY transcription factor 22 [Cucumis melo var. makuwa]	[more]
XP_008437986.1	3.72e-218	92.33	PREDICTED: WRKY transcription factor 22 [Cucumis melo]	[more]
XP_038880705.1	2.46e-206	89.64	WRKY transcription factor 22 [Benincasa hispida]	[more]
XP_023528010.1	5.89e-180	82.49	WRKY transcription factor 22-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G322 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
E7CEW7	4.94e-240	100.00	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY18 PE=2 SV=1	[more]
A0A0A0L7C1	9.21e-238	99.70	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G121580 PE=4 S...	[more]
A0A5D3D3F9	4.59e-219	92.63	WRKY transcription factor 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3AVV5	1.80e-218	92.33	WRKY transcription factor 22 OS=Cucumis melo OX=3656 GN=LOC103483243 PE=4 SV=1	[more]
A0A6J1EEC7	4.05e-180	82.49	WRKY transcription factor 22 OS=Cucurbita moschata OX=3662 GN=LOC111431800 PE=4 ...	[more]

BLAST of Cucsat.G322 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01250.1	8.0e-58	47.29	WRKY family transcription factor	[more]
AT5G52830.1	2.6e-40	38.76	WRKY DNA-binding protein 27	[more]
AT2G34830.1	5.5e-35	52.67	WRKY DNA-binding protein 35	[more]
AT4G23550.1	5.4e-30	39.27	WRKY family transcription factor	[more]
AT1G30650.1	4.5e-29	36.33	WRKY DNA-binding protein 14	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 35..83 e-value: 9.2E-12 score: 55.1
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 41..82 e-value: 8.0E-14 score: 51.6
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 42..84 score: 21.252764
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 38..85 e-value: 1.5E-15 score: 58.7
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 42..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..110
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..132
None	No IPR available	PANTHER	PTHR32096:SF97	WRKY TRANSCRIPTION FACTOR 22-LIKE	coord: 43..190
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR32096	WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED	coord: 43..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G322.T1	Cucsat.G322.T1	mRNA
Cucsat.G322.T3	Cucsat.G322.T3	mRNA
Cucsat.G322.T2	Cucsat.G322.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding