Cucsat.G3072 (gene) Cucumber (B10) v3

Overview
NameCucsat.G3072
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionRHOMBOID-like protein
Locationctg1041: 7342622 .. 7344886 (-)
RNA-Seq ExpressionCucsat.G3072
SyntenyCucsat.G3072
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATAACGACCATCGTAGCAAGAATCGTTCTTTGCCATTCTTTGGCTATTTCTCCTTTCTTTCTCTCACTCTAACAACTGGTGCACAATAGCATCCGTCGTAGCCGACGCTATTGTGCACTCCTCCCCCTCCGTTGGTTGTTGGTTGTTGCCGTCACCGCCGTGCAATGGGGAAGAGACCACCGTCGGTCCACGTGGAATCAATGTCCTATGACGTCCACAAGCCCATCTCAATGCCACCGCCACCCAGCTATTATCCTCCCCCGCCCAAACCATGGTTCCCATGGCTGGTGCCTCTCATTTTTCTAGTCAATGTGGGCATGTTTATTTTCATGTTATATGAAAATAATTGCCCCGCCATCTCTGGAAGAAACCGCTGCCTTCTTTACCCTGAGCTCGGCCGATTCTCCTTCCAACCGTTTCATGAGAATCCCCTCCTCGGCCCTTCCATCACCATGTAAGAATAATATTATTCGTTTGTATGGAACATTAGGATATGGGTTTTGTGAATGGGGGATGGAATTGATTGACCCATGAAGAGAAAAAAATGAATCAGGGAGGAAAAAAACATAGAGGGAATTTTGTTATTTTTGTGATGGTTGCTGGTTTGATTTGTTTTTTCTTTGGTTCTGCAGTTTGAAAAGATATGGGGCTTTGGACAAAGAAGCAGTATTAGAACGTGGAGAAGGTTGGCGCTTGGTGTCTTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGCCTCGAACAAGAATTCGGATTTTGTAAGTACTCTCAAACACATATAAGACGTGTTTGAAATGAGTTTTCGAATGTTTAAAAACACTTTTAGTCGTTCTAGACAAACTCGTAATCTATTTTGTATTGGTTTATCATTGTTTCATTGTGTCTTCGCTCTTAGCATTCATTTTCCACCTAGAAGAGAAGAGATTTTGCCCGTTTTTCTTACTTTTTAACATGAATTTGATTTTGTTTTCTTCTAGAATTTCTCAGCTCACTCTTGGTTATGTAACAAAACAGTACAAAAATCTTAAAATGGAAAATAAAAAGTGTTAAAAAAGTCATCAGTTATACTTTTAGAAAGAGTTAAAATAACTCATATAATGTTAAAAAATTGTTCGAACTGAACCCACAGTTGTATCAAAGAAAAGCTGAACCCATTTGGGATTTTGTTTTTTGTTTTAATGTGCTGAGACATTTGGGATGATTTGTTTATATTTTTGGTGTTTGGCAGTGAGAATAGGATGTTTATATGTACTTTCTGGATTTGGTGGGAGTTTACTTTCATCTATAAGTCTCAATCCAGCTGAAAATCCAACCATATCAGTTGGTGCTTCAGGTGCTCTTTTTGGCCTTTTGGGAGCTATGCTTTCTGAGCTCATTACAAATTGGACAATTTATGCAAATAAGGTAAAACTTAAAACCTTTTCTTTTCCTTCCATTGTTCTTTAAATCTATTTCAACTTATATGGTTTCTTTGTCATTCAAACTCAGTGTGCAGCCCTGTTGTCATTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGTTTCATCCCACACGTCGATAATTCCGCTCACATCGGAGGCTTCATTTCGGGTTTTCTCCTTGGTTTCATCCTCTTAATCCGACCGCAATTCGGATACGTTAACCAGAAGTACATCCCTGCAGGCTCTGACGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACCTTTTGTTGATTATAGCTCTACTCCTCTTGGTTTTTGGGTAATTGATTCGTGACTAAGAAATTACACACTCAACAATGATATATTTTTAGTTTTGTTTTCGCTAACTTTTGTTTACTTTTTATTTACGCAGATATGTTTCTGGATTAACAAAGTTGTATAGAACTGATCTACAAGTATCAAAAAACAACCGTTTCTAAAAGATAACTTTGAATTGTTTGATCAACTTGATAATCGTATTCCTCTCAAATGCCAAATTGATGAAGCATTTGAAAATTTAAATGAAAACGAACCTTATTTTTCTTTTCTTTTTTCTCGTGTACTTGTAAGATAAAAAATCATTTTAATGTGAAGTTTTAATTTCTTCTCATTCATACAGTAGTAGAAGAGTAGTATGTAGAAAAATACTCACTTCTTTGTTTACAAAGGAGGACTTCCCTTTTTCCTTTTCTTTTCCCTCTTAAATTCTATTAGATTTCTGTTTCTTTTTTTTTTTTTTTTTTTCTAATCTCTAACATGC

Coding sequence (CDS)

ATGGGGAAGAGACCACCGTCGGTCCACGTGGAATCAATGTCCTATGACGTCCACAAGCCCATCTCAATGCCACCGCCACCCAGCTATTATCCTCCCCCGCCCAAACCATGGTTCCCATGGCTGGTGCCTCTCATTTTTCTAGTCAATGTGGGCATGTTTATTTTCATGTTATATGAAAATAATTGCCCCGCCATCTCTGGAAGAAACCGCTGCCTTCTTTACCCTGAGCTCGGCCGATTCTCCTTCCAACCGTTTCATGAGAATCCCCTCCTCGGCCCTTCCATCACCATTTTGAAAAGATATGGGGCTTTGGACAAAGAAGCAGTATTAGAACGTGGAGAAGGTTGGCGCTTGGTGTCTTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGCCTCGAACAAGAATTCGGATTTTTGAGAATAGGATGTTTATATGTACTTTCTGGATTTGGTGGGAGTTTACTTTCATCTATAAGTCTCAATCCAGCTGAAAATCCAACCATATCAGTTGGTGCTTCAGGTGCTCTTTTTGGCCTTTTGGGAGCTATGCTTTCTGAGCTCATTACAAATTGGACAATTTATGCAAATAAGTGTGCAGCCCTGTTGTCATTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGTTTCATCCCACACGTCGATAATTCCGCTCACATCGGAGGCTTCATTTCGGGTTTTCTCCTTGGTTTCATCCTCTTAATCCGACCGCAATTCGGATACGTTAACCAGAAGTACATCCCTGCAGGCTCTGACGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACCTTTTGTTGATTATAGCTCTACTCCTCTTGGTTTTTGGATATGTTTCTGGATTAACAAAGTTGTATAGAACTGATCTACAAGTATCAAAAAACAACCGTTTCTAA

Protein sequence

MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSGLTKLYRTDLQVSKNNRF
Homology
BLAST of Cucsat.G3072 vs. ExPASy Swiss-Prot
Match: Q9SSR0 (RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.3e-96
Identity = 183/307 (59.61%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKRPP      +  D+      P  P + PP P PW  WLVPLI   N   F   +Y N
Sbjct: 1   MGKRPP------IPPDIENGPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTMYVN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           +CPA S  + CLL+  LGR SFQP  EN LLGPSI  L++ GAL++  V E GE WRL+S
Sbjct: 61  DCPARS--DECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERRLV-EEGERWRLIS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           C+WLH G +HL+ANM+SL+ IG+RLEQEFGF+RIG LYV+SG GGSL+S   L  ++   
Sbjct: 121 CIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVS--CLTDSQGER 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           +SVGASGALFGLLGAMLSELITNWTIY NKC AL++LILII LNL+VGF+P VDNSAH G
Sbjct: 181 VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSAHFG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVK-RKSKHKCYQYLLLIIALLLLVFGYVS 300
           GF++GF LGF+LL+RPQ+GYVN KYIP G D+K +KSKHKCYQ++    +L +L+ G+++
Sbjct: 241 GFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAGFIA 296

Query: 301 GLTKLYR 307
           G TKL R
Sbjct: 301 GYTKLLR 296

BLAST of Cucsat.G3072 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.5e-90
Identity = 178/279 (63.80%), Postives = 210/279 (75.27%), Query Frame = 0

Query: 33  PPKPWFPWLVPLIFLVNVGMFIFMLYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLG 92
           P K WFPWLVP I + N+ +F   ++ NNCP  S      L   LGRF+FQP  ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA---YCLARFLGRFAFQPMKENPLLG 109

Query: 93  PSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS   L++ GALD   V+ + E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 RIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCA 212
           RIG LY++SGFGGSLLSS+         ISVGASGALFGLLGAMLSEL+TNWTIYANK A
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 ALLSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAG-SD 272
           ALL+LI IIA+NLAVG +PHVDN AH+GGF SGFLLGF+ LIRPQ+GY NQ+  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYLLLIIALLLLVFGYVSGLTKLYR-TDL 310
              KSKHK YQY+L I +L+LL+ GY +GL  L R TDL
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDL 321

BLAST of Cucsat.G3072 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.3e-82
Identity = 151/271 (55.72%), Postives = 205/271 (75.65%), Query Frame = 0

Query: 37  WFPWLVPLIFLVNVGMFIFMLYENNCP-AISGRNRCLLYPELGRFSFQPFHENPLLGPSI 96
           W  WL+P I + N+ +FI +++ N+CP  I+G N+  +   LGRFSFQP  ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  TILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIG 156
           + L++ GAL+   V+   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+R+G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALL 216
            +Y++SG GGS+LSS+ L      +ISVGASGALFGLLGAMLSEL+TNWTIYANK AAL+
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRK 276
           +L+ IIA+NLA+G +P VDN AHIGGF++GF LGF+LL+RPQ+G+   +     +  + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR----TNTSRTK 270

Query: 277 SKHKCYQYLLLIIALLLLVFGYVSGLTKLYR 307
            K+  YQY+L +++++LLV G    L  L++
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

BLAST of Cucsat.G3072 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 154/274 (56.20%), Postives = 208/274 (75.91%), Query Frame = 0

Query: 37  WFPWLVPLIFLVNVGMFIFMLYENNCP----AISGRNRCLLYPELGRFSFQPFHENPLLG 96
           W  WLVP+  + NV +F+  ++ NNCP    +   R  C+    LGR SF+P   NPL G
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVA-KFLGRLSFEPLRTNPLFG 112

Query: 97  PSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 156
           PS   L++ GAL+   V+E+ EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF+
Sbjct: 113 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 172

Query: 157 RIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCA 216
           RIG +Y+LSG GGS+LSS+ +      +ISVGASGALFGLLG+MLSEL TNWTIY+NK A
Sbjct: 173 RIGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIA 232

Query: 217 ALLSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDV 276
           ALL+L+ +I +NLA+G +PHVDN AH+GGF++GFLLGFILL RPQF ++ ++++P G+ +
Sbjct: 233 ALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL 292

Query: 277 KRKSKHKCYQYLLLIIALLLLVFGYVSGLTKLYR 307
             + K+K YQYLL +++L+LL+ G+V  L  L+R
Sbjct: 293 --RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

BLAST of Cucsat.G3072 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 9.8e-79
Identity = 149/271 (54.98%), Postives = 195/271 (71.96%), Query Frame = 0

Query: 35  KPWFPWLVPLIFLVNVGMFIFMLYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPS 94
           + WFPWL+P   + NV +F+  +Y NNCP  SG         LGRFSFQ   ENPLLGPS
Sbjct: 62  RSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGD---CFADFLGRFSFQNTRENPLLGPS 121

Query: 95  ITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRI 154
              L+  G LD + V++  EGWRL+SC WLH GV+HLL NML+LLFIGIR+E+EFGF+RI
Sbjct: 122 SLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRI 181

Query: 155 GCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCAAL 214
           G LY++SGFGGS+LS++ L       ISVGASGA+FGLLG MLSE+  NWTIY+NK   +
Sbjct: 182 GLLYLISGFGGSILSALFL----RSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTI 241

Query: 215 LSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKR 274
           ++L+LI+A+NL +G +P VDN AHIGGF +GFLLGF+LLIRP +G++NQ+  P      +
Sbjct: 242 VTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGPGA----K 301

Query: 275 KSKHKCYQYLLLIIALLLLVFGYVSGLTKLY 306
             + K YQ +L  I+LL+LV G++ GL  L+
Sbjct: 302 PHRFKIYQGILWTISLLILVAGFIVGLISLF 321

BLAST of Cucsat.G3072 vs. NCBI nr
Match: XP_011659795.2 (RHOMBOID-like protein 5 [Cucumis sativus] >KAE8646443.1 hypothetical protein Csa_015571 [Cucumis sativus])

HSP 1 Score: 637 bits (1644), Expect = 1.33e-230
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN
Sbjct: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS
Sbjct: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300
           GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300

Query: 301 LTKLYRTDLQVSKNNRF 317
           LTKLYRTDLQVSKNNRF
Sbjct: 301 LTKLYRTDLQVSKNNRF 317

BLAST of Cucsat.G3072 vs. NCBI nr
Match: KAA0058759.1 (RHOMBOID-like protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 591 bits (1523), Expect = 3.97e-212
Identity = 291/319 (91.22%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPIS-MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYE 60
           MGKRPPSV +ESMSY++HKPI+ MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIF LY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLV 120
           NNCPAI+G NRCLLYPELGRFSFQPFHENPLLGP+I+IL+RYGALDKEAV+ERGEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFL+IGCLYVLSGFGGSLLSSISLNPAENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHI 240
           TISVGASGALFGLLGAMLSELITNWTIYANKCAAL+SLILIIALNLAVGF+PHVDNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVS 300
           GGFISGFLLGFILLIRPQFGYVN KYIP GSDVKRKSKHKCYQY LLI+ALLLLVFGY+S
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFGYIS 300

Query: 301 GLTKLYRTD-LQVSKNNRF 317
           GLTKLYRT  +QVSK+NRF
Sbjct: 301 GLTKLYRTGPIQVSKSNRF 319

BLAST of Cucsat.G3072 vs. NCBI nr
Match: XP_038899775.1 (RHOMBOID-like protein 5 [Benincasa hispida])

HSP 1 Score: 570 bits (1470), Expect = 4.09e-204
Identity = 283/317 (89.27%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKRPPSV  ESMSYDVHKP+SMPPPPSYYPPPPKPWFPWLVPLIF+ NV MFIF LYEN
Sbjct: 1   MGKRPPSV--ESMSYDVHKPMSMPPPPSYYPPPPKPWFPWLVPLIFIANVAMFIFTLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPA +GRN CLLYPELGRFSFQPF ENPLLGPS+ IL+ YGALDKEAV+ERGEGWRLVS
Sbjct: 61  NCPATTGRNHCLLYPELGRFSFQPFQENPLLGPSMNILRIYGALDKEAVVERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           C+WLHAGVIHL+ANMLSLLFIGIRLEQEFGFL+IGCLYVLSGFGGSLLSS+SLN AE+PT
Sbjct: 121 CIWLHAGVIHLIANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSLNSAESPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILII LNLAVGFIPHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIGLNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300
           GFISGFLLGFILLIRPQFGYVN KYIP GSDVKRKSKHKCYQY LLIIALLLL+FGY SG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNHKYIPVGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300

Query: 301 LTKLYRTDLQVSKNNRF 317
           LTKLYRT  QV  +N F
Sbjct: 301 LTKLYRTGPQVLTSNIF 315

BLAST of Cucsat.G3072 vs. NCBI nr
Match: XP_023000373.1 (RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 561 bits (1446), Expect = 2.00e-200
Identity = 270/317 (85.17%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKR P   +ESMSYDV KP+SMPPPPSYYPPPPKPWFPWLVPL FL N+ MF+F LYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPA  G NRCL YPELGRFSFQPF ENPLLGPSI+ LKRYGALDKEAV+E GEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL+IGCLYVLSGFGGSLLSS+S+NP+ENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NKCAA +SLILIIALNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVN KYIPAG+DVKRKSKHKCYQY LLI+AL+LL+FGYVSG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTKLYRTDLQVSKNNRF 317
           LT+LY+T  QV ++N F
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of Cucsat.G3072 vs. NCBI nr
Match: XP_008461110.1 (PREDICTED: RHOMBOID-like protein 5 [Cucumis melo])

HSP 1 Score: 560 bits (1442), Expect = 3.88e-200
Identity = 274/297 (92.26%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPIS-MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYE 60
           MGKRPPSV +ESMSY++HKPI+ MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIF LY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLV 120
           NNCPAI+G NRCLLYPELGRFSFQPFHENPLLGP+I+IL+RYGALDKEAV+ERGEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFL+IGCLYVLSGFGGSLLSSISLNPAENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHI 240
           TISVGASGALFGLLGAMLSELITNWTIYANKCAAL+SLILIIALNLAVGF+PHVDNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296
           GGFISGFLLGFILLIRPQFGYVN KYIP GSDVKRKSKHKCYQY LLI+ALLLLVFG
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFG 297

BLAST of Cucsat.G3072 vs. ExPASy TrEMBL
Match: A0A0A0K8T6 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 8.53e-215
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN
Sbjct: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS
Sbjct: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296
           GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296

BLAST of Cucsat.G3072 vs. ExPASy TrEMBL
Match: A0A5A7UZ09 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002320 PE=3 SV=1)

HSP 1 Score: 591 bits (1523), Expect = 1.92e-212
Identity = 291/319 (91.22%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPIS-MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYE 60
           MGKRPPSV +ESMSY++HKPI+ MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIF LY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLV 120
           NNCPAI+G NRCLLYPELGRFSFQPFHENPLLGP+I+IL+RYGALDKEAV+ERGEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFL+IGCLYVLSGFGGSLLSSISLNPAENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHI 240
           TISVGASGALFGLLGAMLSELITNWTIYANKCAAL+SLILIIALNLAVGF+PHVDNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVS 300
           GGFISGFLLGFILLIRPQFGYVN KYIP GSDVKRKSKHKCYQY LLI+ALLLLVFGY+S
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFGYIS 300

Query: 301 GLTKLYRTD-LQVSKNNRF 317
           GLTKLYRT  +QVSK+NRF
Sbjct: 301 GLTKLYRTGPIQVSKSNRF 319

BLAST of Cucsat.G3072 vs. ExPASy TrEMBL
Match: A0A6J1KMF5 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1)

HSP 1 Score: 561 bits (1446), Expect = 9.67e-201
Identity = 270/317 (85.17%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKR P   +ESMSYDV KP+SMPPPPSYYPPPPKPWFPWLVPL FL N+ MF+F LYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPA  G NRCL YPELGRFSFQPF ENPLLGPSI+ LKRYGALDKEAV+E GEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL+IGCLYVLSGFGGSLLSS+S+NP+ENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NKCAA +SLILIIALNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVN KYIPAG+DVKRKSKHKCYQY LLI+AL+LL+FGYVSG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTKLYRTDLQVSKNNRF 317
           LT+LY+T  QV ++N F
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of Cucsat.G3072 vs. ExPASy TrEMBL
Match: A0A1S3CDZ1 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103499793 PE=3 SV=1)

HSP 1 Score: 560 bits (1442), Expect = 1.88e-200
Identity = 274/297 (92.26%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPIS-MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYE 60
           MGKRPPSV +ESMSY++HKPI+ MPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIF LY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLV 120
           NNCPAI+G NRCLLYPELGRFSFQPFHENPLLGP+I+IL+RYGALDKEAV+ERGEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFL+IGCLYVLSGFGGSLLSSISLNPAENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHI 240
           TISVGASGALFGLLGAMLSELITNWTIYANKCAAL+SLILIIALNLAVGF+PHVDNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296
           GGFISGFLLGFILLIRPQFGYVN KYIP GSDVKRKSKHKCYQY LLI+ALLLLVFG
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFG 297

BLAST of Cucsat.G3072 vs. ExPASy TrEMBL
Match: A0A6J1HKQ1 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1)

HSP 1 Score: 558 bits (1439), Expect = 1.13e-199
Identity = 269/317 (84.86%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKR P   +ESMSY+V KP+SMPPPPSYYPPPPKPWFPWLVPL FL N+ MF F LYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFTLYEN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           NCPA  G NRCL YPELGRFSFQPF ENPLLGPSI+ LKRYGALDKEAV+E GEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL+IGCLYVLSGFGGSLLSS+S+NP+ENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NKCAA +SLILIIALNLAVGFIPH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFGYVSG 300
           GF+SGFLLGFILLI+PQFGYVN KYIPAG+DVKRKSKHKCYQY LLI+AL+LL+FGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTKLYRTDLQVSKNNRF 317
           LT+LY+T  QV ++N F
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of Cucsat.G3072 vs. TAIR 10
Match: AT1G52580.1 (RHOMBOID-like protein 5 )

HSP 1 Score: 353.6 bits (906), Expect = 1.7e-97
Identity = 183/307 (59.61%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60
           MGKRPP      +  D+      P  P + PP P PW  WLVPLI   N   F   +Y N
Sbjct: 1   MGKRPP------IPPDIENGPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTMYVN 60

Query: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120
           +CPA S  + CLL+  LGR SFQP  EN LLGPSI  L++ GAL++  V E GE WRL+S
Sbjct: 61  DCPARS--DECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERRLV-EEGERWRLIS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180
           C+WLH G +HL+ANM+SL+ IG+RLEQEFGF+RIG LYV+SG GGSL+S   L  ++   
Sbjct: 121 CIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVS--CLTDSQGER 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240
           +SVGASGALFGLLGAMLSELITNWTIY NKC AL++LILII LNL+VGF+P VDNSAH G
Sbjct: 181 VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSAHFG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVK-RKSKHKCYQYLLLIIALLLLVFGYVS 300
           GF++GF LGF+LL+RPQ+GYVN KYIP G D+K +KSKHKCYQ++    +L +L+ G+++
Sbjct: 241 GFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAGFIA 296

Query: 301 GLTKLYR 307
           G TKL R
Sbjct: 301 GYTKLLR 296

BLAST of Cucsat.G3072 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 178/279 (63.80%), Postives = 210/279 (75.27%), Query Frame = 0

Query: 33  PPKPWFPWLVPLIFLVNVGMFIFMLYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLG 92
           P K WFPWLVP I + N+ +F   ++ NNCP  S      L   LGRF+FQP  ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA---YCLARFLGRFAFQPMKENPLLG 109

Query: 93  PSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS   L++ GALD   V+ + E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 RIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCA 212
           RIG LY++SGFGGSLLSS+         ISVGASGALFGLLGAMLSEL+TNWTIYANK A
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 ALLSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAG-SD 272
           ALL+LI IIA+NLAVG +PHVDN AH+GGF SGFLLGF+ LIRPQ+GY NQ+  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYLLLIIALLLLVFGYVSGLTKLYR-TDL 310
              KSKHK YQY+L I +L+LL+ GY +GL  L R TDL
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDL 321

BLAST of Cucsat.G3072 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 312.8 bits (800), Expect = 3.2e-85
Identity = 172/296 (58.11%), Postives = 203/296 (68.58%), Query Frame = 0

Query: 33  PPKPWFPWLVPLIFLVNVGMFIFMLYENNCPAISGRNRCLLYPELGRFSFQPFHENPLLG 92
           P K WFPWLVP I + N+ +F   ++ NNCP  S      L   LGRF+FQP  ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA---YCLARFLGRFAFQPMKENPLLG 109

Query: 93  PSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS   L++ GALD   V+ + E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 RIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKC- 212
           RIG LY++SGFGGSLLSS+         ISVGASGALFGLLGAMLSEL+TNWTIYANK  
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKVA 229

Query: 213 ---------------------------AALLSLILIIALNLAVGFIPHVDNSAHIGGFIS 272
                                      AALL+LI IIA+NLAVG +PHVDN AH+GGF S
Sbjct: 230 KSSLVKQAALSMNDVSIMSLVFLHLQFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTS 289

Query: 273 GFLLGFILLIRPQFGYVNQKYIPAG-SDVKRKSKHKCYQYLLLIIALLLLVFGYVS 300
           GFLLGF+ LIRPQ+GY NQ+  P G +    KSKHK YQY+L I +L+LL+ GY S
Sbjct: 290 GFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGYTS 338

BLAST of Cucsat.G3072 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 305.4 bits (781), Expect = 5.2e-83
Identity = 151/271 (55.72%), Postives = 205/271 (75.65%), Query Frame = 0

Query: 37  WFPWLVPLIFLVNVGMFIFMLYENNCP-AISGRNRCLLYPELGRFSFQPFHENPLLGPSI 96
           W  WL+P I + N+ +FI +++ N+CP  I+G N+  +   LGRFSFQP  ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  TILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIG 156
           + L++ GAL+   V+   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+R+G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCAALL 216
            +Y++SG GGS+LSS+ L      +ISVGASGALFGLLGAMLSEL+TNWTIYANK AAL+
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRK 276
           +L+ IIA+NLA+G +P VDN AHIGGF++GF LGF+LL+RPQ+G+   +     +  + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR----TNTSRTK 270

Query: 277 SKHKCYQYLLLIIALLLLVFGYVSGLTKLYR 307
            K+  YQY+L +++++LLV G    L  L++
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

BLAST of Cucsat.G3072 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 304.7 bits (779), Expect = 8.8e-83
Identity = 154/274 (56.20%), Postives = 208/274 (75.91%), Query Frame = 0

Query: 37  WFPWLVPLIFLVNVGMFIFMLYENNCP----AISGRNRCLLYPELGRFSFQPFHENPLLG 96
           W  WLVP+  + NV +F+  ++ NNCP    +   R  C+    LGR SF+P   NPL G
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVA-KFLGRLSFEPLRTNPLFG 112

Query: 97  PSITILKRYGALDKEAVLERGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 156
           PS   L++ GAL+   V+E+ EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF+
Sbjct: 113 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 172

Query: 157 RIGCLYVLSGFGGSLLSSISLNPAENPTISVGASGALFGLLGAMLSELITNWTIYANKCA 216
           RIG +Y+LSG GGS+LSS+ +      +ISVGASGALFGLLG+MLSEL TNWTIY+NK A
Sbjct: 173 RIGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIA 232

Query: 217 ALLSLILIIALNLAVGFIPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNQKYIPAGSDV 276
           ALL+L+ +I +NLA+G +PHVDN AH+GGF++GFLLGFILL RPQF ++ ++++P G+ +
Sbjct: 233 ALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL 292

Query: 277 KRKSKHKCYQYLLLIIALLLLVFGYVSGLTKLYR 307
             + K+K YQYLL +++L+LL+ G+V  L  L+R
Sbjct: 293 --RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SSR02.3e-9659.61RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1[more]
Q0WQX72.5e-9063.80RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
Q9CAN17.3e-8255.72RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
Q9LYP11.2e-8156.20RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
F4JBM49.8e-7954.98RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011659795.21.33e-230100.00RHOMBOID-like protein 5 [Cucumis sativus] >KAE8646443.1 hypothetical protein Csa... [more]
KAA0058759.13.97e-21291.22RHOMBOID-like protein 5 [Cucumis melo var. makuwa][more]
XP_038899775.14.09e-20489.27RHOMBOID-like protein 5 [Benincasa hispida][more]
XP_023000373.12.00e-20085.17RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima][more]
XP_008461110.13.88e-20092.26PREDICTED: RHOMBOID-like protein 5 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0K8T68.53e-215100.00RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1[more]
A0A5A7UZ091.92e-21291.22RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold33... [more]
A0A6J1KMF59.67e-20185.17RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1[more]
A0A1S3CDZ11.88e-20092.26RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103499793 PE=3 SV=1[more]
A0A6J1HKQ11.13e-19984.86RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52580.11.7e-9759.61RHOMBOID-like protein 5 [more]
AT2G29050.11.8e-9163.80RHOMBOID-like 1 [more]
AT2G29050.23.2e-8558.11RHOMBOID-like 1 [more]
AT1G63120.15.2e-8355.72RHOMBOID-like 2 [more]
AT5G07250.18.8e-8356.20RHOMBOID-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 38..255
e-value: 2.0E-38
score: 133.9
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 35..257
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 111..255
e-value: 2.7E-42
score: 144.3
NoneNo IPR availablePANTHERPTHR22936:SF87RHOMBOID-LIKE PROTEIN 5coord: 1..306
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 1..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G3072.T1Cucsat.G3072.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity