Cucsat.G3016 (gene) Cucumber (B10) v3

Overview
NameCucsat.G3016
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionSpoU_methylase domain-containing protein
Locationctg1041: 6580967 .. 6583637 (-)
RNA-Seq ExpressionCucsat.G3016
SyntenyCucsat.G3016
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTAAATAATAATTATAAAAATAGCGAGTGTTCTTCCTTTGAAACGAACTTAGTGAGGCTCTCGAGCGGCAACAAGAAGAAGGAAGAAGCAGAAGATAGTGTTGCTGAGATGCCGTTAATGGACACAACAACAGCCACTCTCAGAGCTTTAAACCCATTTACCCCTTTTCAACTACGCTCATCCAGGTCTCTTCATCTTCTTCGCCCTCGCTCCTTCTCCCTTTTCACCAACAAACACCACAATCTATCTTCTTCATCCTTACGCCGGTTACTCCCCTTCTGCTCCTCATGTAAGTATATATGATACATCTGTAATTAAACGTTCTTACATCCTATGGACTCTATAATCTCTGCTGTGAATTGGGTTGAAGTTATTTTCTTCAGTTTGATTTTGCTGTGTTCATGGCGCAGCTGACAGAGTGGAGGGTTCTGGTTCCCTACCGACCGGTGTTGGTGAGGCCGTCAATGGGGTCTCGCATCCAAAGCTTCTTCAAGTTGTATTGGTGTCTCCCTTGGTAATTGCTTAGCTGATTCTTTTTCTTGCTTTTCGTTTCTGCATTTTTTTTTCTAGTAATCGCTATTTTTAGTTATTGTTTGTGTTCTTATCTGTATTTGGTGGAGTTGTATGGCTTGATTTAATATTTTGAATAAGTTTCACTTTGCTTTAAGATTTTGTTTGATTGTAAAGGATGATATTTCATACTATTGGAAAATTTGATCAAATGTAAACTGTACATTTAAGAACTGCAGTCAATTGAGTGGTATGACTTCGAACCTTGGATTGAGCTTAATAAGAAAAAAAAAACCCTGTTTGGGAGATTGAAATGTAAATTACGATGAAATGTTTAATTCGGTGTTAAATTGGGATTGTGAAACGGGAAAAATGGGAGAAAGAGAGTTGAGTTGAAAATAATGGAAAAGGAATATAAAAGGAGTTACAAACCAGGCTCAAAACCCTAACCATCAAATTCTAGCTTCCCAAACATGTTCATTGAATCTAGGAATTGGGTTAGACTCGAGCTTGGTTGCCCTCGAATTAATGGGGCTAAGGCCCTCGATACCGTGTTACTAAAACAAACATATATATGAAACTCAGAGGATATTAGCACATGGAAATTTGCTGACATTTTACATGGTATTTTGTTTGAAACCGAATGAACAAGGACTAGGGAGCCTTTAGTTAGATCTTTGCATCAATCTTAGGTGGTATTCTTGGATGACTAACAAGTTGAAATTAGTAGAGCTGCTTGGGAACAGTCTTCTTCAAGTCGAAATGTGAGGGTTGAGCAAAACCTCTCATAGAAATTTTAATAGATTGCTTGAAAACTTGCTAGGCGTATTATGGTTGTTTATGTCATATATAGCAAAAGGTCATGTGATGTTAATCGTAAACTTTTATTTTGGTTAGTATCAAATGCTTGGAGCAAATTTCTTCCAAATTGATTTCTACTTCTAATGAAATACACACTCGACATTGTGTGTACACATACGGCACCGCTTATTAGTTTGTATATCACGGCACGTTGTATGCAGTTGTGTGAAACACGGTGAACGCCCAATTGTAATTTCTCCATTTGCCTTTTTGGGTTGCAGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTGTGTTGTCCTTGGCAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCTGCATCGGCTGTAGGACTACATCTAGTTGGGGTTAGTATTGTATCATCTTTACTTTAAGCATAATCGTTCTGACTTTAATAACTTATCCACTTGTATTGTCTCCTTCTCAGCCATTGGGATTTAAGGTTGATGACACAAAGCTAAAGCGGGCAGGTTTGGATTATTGGCCGTATCCCATGATGCATTTTATATATGATTCATTTGCTCTCATTTACAGTTAAATATCAGGGATTCAATGTTAGGAAATTGTGCTTTACTTTGTCTTGAACTGTTTGAGTTTTCTATTTCGTGCTGTCACTGTTCGTTCAATGTTTTCGGGTTTTAACATTGTATTTTAGATACGTTGTCGTTAAAATTCATAACACGTGGGCGGATTTTCAAGAATACTTTAAGAAGCAGGTAAGCATGGACAAGATTCCATTTTAGTTTCCTCTTTGCATCCTACATTGTTGGAAACTATGTCTACCAACTCAAATGATATGATATGTACAGGAAGGAGAAAAGCGGCTGCTAGCATTTACAAAAAGAGGAACAACACATCACTCGGCGAGTGCCTATTTCTCTGACGATTTGATTTCTATACGAGTCGTCAATTGCTCATAGGCCAGTAGGAATTTTGAGTAACAATTTGTTTTCTCATTTCATTTCTGCAGGACTTTTCGTACAAGCGGGGCGATTACTTGTTGTTCGGCTCAGAAACTACTGGATTACCACCTGAAGCGCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACATTAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTCTCGGTGAGTGTTGGTATTGCTATATACGAAGCTTCAAGACAACTAAATTACGAGCAACTTCAGGTTTCATCAGAGAGTTGTACTAACAATGAACAATCTATTATTACAGAGGATGTTTTTGCTTGATCCTTAGATGTGAAATTTTGTACCATTTTAATTTTATTTGGAGACTCTCAAAGAAACATTGTTTACCTTATGATATTAAAGCCAATTCATTGCATGATGCTGTTATGTTACTATTTTGTTATACTTTGTAATGTTTACTTGGATTTCACATTACTTTAATGCTACAATTTTCTCATATATTATTTGT

Coding sequence (CDS)

CCTTTAAATAATAATTATAAAAATAGCGAGTGTTCTTCCTTTGAAACGAACTTAGTGAGGCTCTCGAGCGGCAACAAGAAGAAGGAAGAAGCAGAAGATAGTGTTGCTGAGATGCCGTTAATGGACACAACAACAGCCACTCTCAGAGCTTTAAACCCATTTACCCCTTTTCAACTACGCTCATCCAGGTCTCTTCATCTTCTTCGCCCTCGCTCCTTCTCCCTTTTCACCAACAAACACCACAATCTATCTTCTTCATCCTTACGCCGGTTACTCCCCTTCTGCTCCTCATCTGACAGAGTGGAGGGTTCTGGTTCCCTACCGACCGGTGTTGGTGAGGCCGTCAATGGGGTCTCGCATCCAAAGCTTCTTCAAGTTGTATTGGTGTCTCCCTTGACAACCTCAAAATTCCTCAAGGATGGCTCTGAGATTTGTGTTGTCCTTGGCAGATTCCTGGGAACACTGGTAGCATTGCCAGAACATGTGCTGCATCGGCTGTAG

Protein sequence

PLNNNYKNSECSSFETNLVRLSSGNKKKEEAEDSVAEMPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSSSDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLTTSKFLKDGSEICVVLGRFLGTLVALPEHVLHRL
Homology
BLAST of Cucsat.G3016 vs. ExPASy Swiss-Prot
Match: P74516 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0992 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-09
Identity = 30/47 (63.83%), Postives = 39/47 (82.98%), Query Frame = 0

Query: 115 LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 162
           ++++VLV+P IP NTG+IARTCAA+   LHLVGPLGF++ D  LKRA
Sbjct: 1   MVKLVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFELGDRYLKRA 47

BLAST of Cucsat.G3016 vs. ExPASy Swiss-Prot
Match: O31590 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=cspR PE=3 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 30/46 (65.22%), Postives = 33/46 (71.74%), Query Frame = 0

Query: 116 LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 162
           L VVL  P IP NTG+IARTCAA+   LHL+ PLGF  DD  LKRA
Sbjct: 6   LHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRA 51

BLAST of Cucsat.G3016 vs. ExPASy Swiss-Prot
Match: P32813 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Geobacillus stearothermophilus OX=1422 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.4e-08
Identity = 29/46 (63.04%), Postives = 33/46 (71.74%), Query Frame = 0

Query: 116 LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 162
           L VVL  P IP NTG+IARTCAA+   LHL+ PLGF  DD  L+RA
Sbjct: 3   LHVVLFQPEIPANTGNIARTCAATNTALHLIRPLGFSTDDKMLRRA 48

BLAST of Cucsat.G3016 vs. ExPASy Swiss-Prot
Match: Q48V41 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Streptococcus pyogenes serotype M28 (strain MGAS6180) OX=319701 GN=M28_Spy0301 PE=3 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-08
Identity = 30/56 (53.57%), Postives = 38/56 (67.86%), Query Frame = 0

Query: 118 VVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA-----DTLSLKF 169
           +VL  P IP NTG+IARTCAA+   LH++ P+GF +DD K+KRA     D L L F
Sbjct: 20  IVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHF 75

BLAST of Cucsat.G3016 vs. ExPASy Swiss-Prot
Match: Q0BBL1 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) OX=339670 GN=trmL PE=3 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-07
Identity = 24/47 (51.06%), Postives = 34/47 (72.34%), Query Frame = 0

Query: 115 LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 162
           +  V+LV+P IP NTG++ R CA +   LHL+ PLGF +DD K++RA
Sbjct: 1   MFNVILVAPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRA 47

BLAST of Cucsat.G3016 vs. NCBI nr
Match: KAA0058644.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >TYK10451.1 putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 281 bits (719), Expect = 4.69e-92
Identity = 146/162 (90.12%), Postives = 152/162 (93.83%), Query Frame = 0

Query: 2   ECSSF--ETNLVRLSSGNKKKEEAEDSVAEMPLMDTTTATLRALNPFTPFQLRSSRSLHL 61
           +CSSF  ET+LVRLSSGNKKKEEA DS A+MPLMDTTTATLR LNPFTPFQ RSSRS HL
Sbjct: 19  QCSSFSFETSLVRLSSGNKKKEEAVDSAAKMPLMDTTTATLRTLNPFTPFQRRSSRSHHL 78

Query: 62  LRPRSFSLFTNKHHNLSSSSLRRLLPFCSSSDRVEGSGSLPTGVGEAVNGVSHPKLLQVV 121
           LRP SFSLFTNKHHN SSSS RRLLPFCSSS+RVEGSGSLP+GVGEAVNGVSHPK+LQVV
Sbjct: 79  LRPHSFSLFTNKHHNPSSSSSRRLLPFCSSSERVEGSGSLPSGVGEAVNGVSHPKILQVV 138

Query: 122 LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 161
           LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA
Sbjct: 139 LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 180

BLAST of Cucsat.G3016 vs. NCBI nr
Match: XP_004135918.1 (uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical protein Csa_016173 [Cucumis sativus])

HSP 1 Score: 261 bits (667), Expect = 7.74e-85
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 89
           MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS
Sbjct: 1   MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 60

Query: 90  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 149
           SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL
Sbjct: 61  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 150 GFKVDDTKLKRA 161
           GFKVDDTKLKRA
Sbjct: 121 GFKVDDTKLKRA 132

BLAST of Cucsat.G3016 vs. NCBI nr
Match: XP_008461270.1 (PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo])

HSP 1 Score: 244 bits (622), Expect = 5.24e-78
Identity = 123/132 (93.18%), Postives = 126/132 (95.45%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 89
           MPLMDTTTATLR LNPFTPFQ RSSRS HLLRP SFSLFTNKHHN SSSS RRLLPFCSS
Sbjct: 1   MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 60

Query: 90  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 149
           S+RVEGSGSLP+GVGEAVNGVSHPK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL
Sbjct: 61  SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 150 GFKVDDTKLKRA 161
           GFKVDDTKLKRA
Sbjct: 121 GFKVDDTKLKRA 132

BLAST of Cucsat.G3016 vs. NCBI nr
Match: XP_038898778.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X2 [Benincasa hispida])

HSP 1 Score: 221 bits (562), Expect = 6.96e-69
Identity = 115/133 (86.47%), Postives = 117/133 (87.97%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHN-LSSSSLRRLLPFCS 89
           MPLMDT T TLR LNPF PFQLRSSR  HLLRP  FS FTNK HN  SSSS  RLLPFCS
Sbjct: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60

Query: 90  SSDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 149
            S+RVEGSGSLP+GVGEAVNGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP
Sbjct: 61  LSERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120

Query: 150 LGFKVDDTKLKRA 161
           LGFKVDDTKLKRA
Sbjct: 121 LGFKVDDTKLKRA 133

BLAST of Cucsat.G3016 vs. NCBI nr
Match: XP_038898777.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 213 bits (542), Expect = 9.78e-66
Identity = 114/142 (80.28%), Postives = 118/142 (83.10%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHN-LSSSSLRRLLPFCS 89
           MPLMDT T TLR LNPF PFQLRSSR  HLLRP  FS FTNK HN  SSSS  RLLPFCS
Sbjct: 1   MPLMDTATVTLRTLNPFPPFQLRSSRPHHLLRPHCFSHFTNKQHNPSSSSSFHRLLPFCS 60

Query: 90  ---------SSDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAAS 149
                    +++RVEGSGSLP+GVGEAVNGVS PKLLQVVLVSPLIPGNTGSIARTCAAS
Sbjct: 61  LFWFCCVNGAAERVEGSGSLPSGVGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAAS 120

Query: 150 AVGLHLVGPLGFKVDDTKLKRA 161
           AVGLHLVGPLGFKVDDTKLKRA
Sbjct: 121 AVGLHLVGPLGFKVDDTKLKRA 142

BLAST of Cucsat.G3016 vs. ExPASy TrEMBL
Match: A0A5D3CFA4 (Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001000 PE=3 SV=1)

HSP 1 Score: 281 bits (719), Expect = 2.27e-92
Identity = 146/162 (90.12%), Postives = 152/162 (93.83%), Query Frame = 0

Query: 2   ECSSF--ETNLVRLSSGNKKKEEAEDSVAEMPLMDTTTATLRALNPFTPFQLRSSRSLHL 61
           +CSSF  ET+LVRLSSGNKKKEEA DS A+MPLMDTTTATLR LNPFTPFQ RSSRS HL
Sbjct: 19  QCSSFSFETSLVRLSSGNKKKEEAVDSAAKMPLMDTTTATLRTLNPFTPFQRRSSRSHHL 78

Query: 62  LRPRSFSLFTNKHHNLSSSSLRRLLPFCSSSDRVEGSGSLPTGVGEAVNGVSHPKLLQVV 121
           LRP SFSLFTNKHHN SSSS RRLLPFCSSS+RVEGSGSLP+GVGEAVNGVSHPK+LQVV
Sbjct: 79  LRPHSFSLFTNKHHNPSSSSSRRLLPFCSSSERVEGSGSLPSGVGEAVNGVSHPKILQVV 138

Query: 122 LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 161
           LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA
Sbjct: 139 LVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRA 180

BLAST of Cucsat.G3016 vs. ExPASy TrEMBL
Match: A0A0A0K6L9 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428850 PE=3 SV=1)

HSP 1 Score: 261 bits (667), Expect = 3.75e-85
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 89
           MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS
Sbjct: 1   MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 60

Query: 90  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 149
           SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL
Sbjct: 61  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 150 GFKVDDTKLKRA 161
           GFKVDDTKLKRA
Sbjct: 121 GFKVDDTKLKRA 132

BLAST of Cucsat.G3016 vs. ExPASy TrEMBL
Match: A0A1S3CFJ1 (putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499907 PE=3 SV=1)

HSP 1 Score: 244 bits (622), Expect = 2.54e-78
Identity = 123/132 (93.18%), Postives = 126/132 (95.45%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 89
           MPLMDTTTATLR LNPFTPFQ RSSRS HLLRP SFSLFTNKHHN SSSS RRLLPFCSS
Sbjct: 1   MPLMDTTTATLRTLNPFTPFQRRSSRSHHLLRPHSFSLFTNKHHNPSSSSSRRLLPFCSS 60

Query: 90  SDRVEGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 149
           S+RVEGSGSLP+GVGEAVNGVSHPK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL
Sbjct: 61  SERVEGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPL 120

Query: 150 GFKVDDTKLKRA 161
           GFKVDDTKLKRA
Sbjct: 121 GFKVDDTKLKRA 132

BLAST of Cucsat.G3016 vs. ExPASy TrEMBL
Match: A0A6J1KVT5 (uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049 PE=3 SV=1)

HSP 1 Score: 204 bits (518), Expect = 6.49e-62
Identity = 108/149 (72.48%), Postives = 123/149 (82.55%), Query Frame = 0

Query: 14  SSGNKKKEEAEDSVAEMPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHH 73
           S+  +++ + ED  A+MPLMDTT  TLR L+PF+PF LRSS S  LLR R FS F++KH 
Sbjct: 34  STAEERRRQEEDCSAKMPLMDTTATTLRTLHPFSPFLLRSSTSHRLLRARCFSHFSHKHR 93

Query: 74  NLSSSSLRRLLPFCSSSDRVEGS-GSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSI 133
           +  SSS RRLLPFCS S++ EGS GSLP GVGEA+NGVS  K+LQVVLVSPLIPGNTGSI
Sbjct: 94  HPCSSSSRRLLPFCSFSEKGEGSSGSLPRGVGEALNGVSQQKVLQVVLVSPLIPGNTGSI 153

Query: 134 ARTCAASAVGLHLVGPLGFKVDDTKLKRA 161
           ARTCAASAVGLHLVGPLGFKVDDTKLKRA
Sbjct: 154 ARTCAASAVGLHLVGPLGFKVDDTKLKRA 182

BLAST of Cucsat.G3016 vs. ExPASy TrEMBL
Match: A0A6J1H4X6 (uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC111460609 PE=3 SV=1)

HSP 1 Score: 191 bits (484), Expect = 2.12e-57
Identity = 101/133 (75.94%), Postives = 112/133 (84.21%), Query Frame = 0

Query: 30  MPLMDTTTATLRALNPFTPFQLRSSRSLHLLRPRSFSLFTNKHHNLSSSSLRRLLPFCSS 89
           MPLMDTT  TLR L+PF+PF LR+S S  LLR R FS F++KH +  SSS RRLLPFCS 
Sbjct: 1   MPLMDTTATTLRTLHPFSPFLLRASTSHRLLRARCFSHFSHKHRHPCSSSSRRLLPFCSF 60

Query: 90  SDRVEGS-GSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 149
           S++ EGS GSLP GVG+A+NGVS  K+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP
Sbjct: 61  SEKGEGSSGSLPRGVGKALNGVSQQKVLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGP 120

Query: 150 LGFKVDDTKLKRA 161
           LGFKVDD KLKRA
Sbjct: 121 LGFKVDDKKLKRA 133

BLAST of Cucsat.G3016 vs. TAIR 10
Match: AT5G26880.1 (AGAMOUS-like 26 )

HSP 1 Score: 92.0 bits (227), Expect = 5.3e-19
Identity = 64/122 (52.46%), Postives = 75/122 (61.48%), Query Frame = 0

Query: 49  FQLRSSRSL-------HLLRPRSFSL--FTNKHHNLSSSSLRRLLPFCSSSDRVEGSGSL 108
           F LRSS  L       HL    S S+  F++K   + S +LR +      SD+      L
Sbjct: 14  FHLRSSGRLFPSLSLTHLKGKLSLSINSFSSK---IQSHALRGV--GIGESDK---KNPL 73

Query: 109 PTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLK 162
           P G GE V   +  KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++K
Sbjct: 74  PRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVK 127

BLAST of Cucsat.G3016 vs. TAIR 10
Match: AT5G26880.2 (AGAMOUS-like 26 )

HSP 1 Score: 92.0 bits (227), Expect = 5.3e-19
Identity = 64/122 (52.46%), Postives = 75/122 (61.48%), Query Frame = 0

Query: 49  FQLRSSRSL-------HLLRPRSFSL--FTNKHHNLSSSSLRRLLPFCSSSDRVEGSGSL 108
           F LRSS  L       HL    S S+  F++K   + S +LR +      SD+      L
Sbjct: 14  FHLRSSGRLFPSLSLTHLKGKLSLSINSFSSK---IQSHALRGV--GIGESDK---KNPL 73

Query: 109 PTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLK 162
           P G GE V   +  KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++K
Sbjct: 74  PRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARVK 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P745161.3e-0963.83Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain... [more]
O315901.1e-0865.22Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Bacillus subtilis (strain... [more]
P328131.4e-0863.04Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Geobacillus stearothermop... [more]
Q48V411.8e-0853.57Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Streptococcus pyogenes se... [more]
Q0BBL12.7e-0751.06tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia ambifaria (strain ATC... [more]
Match NameE-valueIdentityDescription
KAA0058644.14.69e-9290.12putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >... [more]
XP_004135918.17.74e-85100.00uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical ... [more]
XP_008461270.15.24e-7893.18PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo][more]
XP_038898778.16.96e-6986.47putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X2 [Benincasa hispid... [more]
XP_038898777.19.78e-6680.28putative tRNA (cytidine(34)-2'-O)-methyltransferase isoform X1 [Benincasa hispid... [more]
Match NameE-valueIdentityDescription
A0A5D3CFA42.27e-9290.12Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa ... [more]
A0A0A0K6L93.75e-85100.00SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428... [more]
A0A1S3CFJ12.54e-7893.18putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1KVT56.49e-6272.48uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049... [more]
A0A6J1H4X62.12e-5775.94uncharacterized protein LOC111460609 OS=Cucurbita moschata OX=3662 GN=LOC1114606... [more]
Match NameE-valueIdentityDescription
AT5G26880.15.3e-1952.46AGAMOUS-like 26 [more]
AT5G26880.25.3e-1952.46AGAMOUS-like 26 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G3016.T3Cucsat.G3016.T3mRNA
Cucsat.G3016.T2Cucsat.G3016.T2mRNA
Cucsat.G3016.T1Cucsat.G3016.T1mRNA
Cucsat.G3016.T8Cucsat.G3016.T8mRNA
Cucsat.G3016.T5Cucsat.G3016.T5mRNA
Cucsat.G3016.T4Cucsat.G3016.T4mRNA
Cucsat.G3016.T7Cucsat.G3016.T7mRNA
Cucsat.G3016.T6Cucsat.G3016.T6mRNA
Cucsat.G3016.T10Cucsat.G3016.T10mRNA
Cucsat.G3016.T11Cucsat.G3016.T11mRNA
Cucsat.G3016.T9Cucsat.G3016.T9mRNA
Cucsat.G3016.T12Cucsat.G3016.T12mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002131 wobble position cytosine ribose methylation
biological_process GO:0002132 wobble position uridine ribose methylation
biological_process GO:0001510 RNA methylation
biological_process GO:0006396 RNA processing
molecular_function GO:0003723 RNA binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity