Cucsat.G301 (gene) Cucumber (B10) v3

BLAST of Cucsat.G301 vs. ExPASy Swiss-Prot
Match: Q76MT4 (ESF1 homolog OS=Rattus norvegicus OX=10116 GN=Esf1 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.2e-47
Identity = 164/477 (34.38%), Postives = 250/477 (52.41%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEE++ GPV L            +D  + +    EKLR Y+  RL+YYYAVVECDS  T
Sbjct: 386 MKEEQIQGPVELL--------SIPEDAPEKDWASREKLRDYQFKRLKYYYAVVECDSPET 445

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAP-SSYEVLNFHTPALQHS 120
           A  +Y+ CDG+EFE S + +DLRFIPD + F+  P+D A+E   ++Y+   F + A+  S
Sbjct: 446 ASKIYEDCDGLEFESSCSFIDLRFIPDDITFDDEPKDAASEVDLTAYKPKYFTSAAMGTS 505

Query: 121 KIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLA--SDESESDDESDDGEDQVD---- 180
            + ++WDE + +R+  L RKF  D+L D++ + +LA  S++ E  +E+ +GED V     
Sbjct: 506 TVEITWDETDHERITTLNRKFKKDELLDMDFEAYLASSSEDEEEVEEAPEGEDGVSIEDG 565

Query: 181 --KKRKKGD-----KYRALLQSDEDGEQDGGQ---DMEVTFNTGL----EDISKRILEKK 240
             KK +K D     KYR LLQ  ++ E+ G +   +ME+ +  GL    E++ K  LE K
Sbjct: 566 KTKKSQKDDEEQIAKYRQLLQVIQEKEKKGKENDMEMEIKWVPGLKESAEEMVKNKLEGK 625

Query: 241 DKKSETLWEAHLRKKHEKRMASRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESG-- 300
           DK   T WE  L KK EK+   + + A + +    +   +V+  D +F EE  VK+ G  
Sbjct: 626 DKL--TPWEQFLEKKKEKKRLKKKQKALAEEASEDEIPSDVDLNDPYFAEE--VKKIGIK 685

Query: 301 KDRTKNIKGREHVGEDGAAEASRAELELLLADDD-------GVDTSIKGYNLKHKKKK-- 360
           K   K+ K      E+   E  +AE+ LL+ D++         D  ++  NL  KKKK  
Sbjct: 686 KKSMKSAKDGATSEEETELEKQKAEMALLVMDEEEDSKKHFNYDKIVEHQNLSKKKKKQL 745

Query: 361 -GKEDITEDKIPTVDYNDPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGYQ 420
             K+++ ED    V+ +D RF A++ S LF LDP+DP FK++ A  + +  K        
Sbjct: 746 MKKKELLEDDF-EVNVSDARFQAMYTSHLFNLDPSDPNFKKTKAMEKILEEK-------- 805

Query: 421 PTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMKSKQLQ 445
             + R  K      A     +  G  + K   D +      LS L+KS+K K++Q Q
Sbjct: 806 -ARHREQKEERLIQAVERAQQDTGKPAQKQPMDPA------LSMLIKSVKNKTEQFQ 834

BLAST of Cucsat.G301 vs. ExPASy Swiss-Prot
Match: Q3V1V3 (ESF1 homolog OS=Mus musculus OX=10090 GN=Esf1 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 6.8e-47
Identity = 163/478 (34.10%), Postives = 250/478 (52.30%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEE++ GPV L            +D  + +    EKLR Y+  RL+YYYAV ECDS  T
Sbjct: 388 MKEEQVQGPVELL--------SIPEDAPEKDWASREKLRDYQFKRLKYYYAVAECDSPET 447

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAP-SSYEVLNFHTPALQHS 120
           A  +Y+ CDG+EFE S + +DLRFIPD + F+  P+D A E   ++Y+   F + A+  S
Sbjct: 448 ASKIYEDCDGLEFESSCSFIDLRFIPDDITFDDEPKDVALEVDLTAYKPKYFTSAAMGTS 507

Query: 121 KIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLA--SDESESDDESDDGEDQVD---- 180
            + ++WDE + +R+  L RKF  D+L D++ + +LA  S++ E  +E+ +GE+ V+    
Sbjct: 508 TVEITWDETDHERITTLNRKFKKDELLDMDFQAYLASSSEDEEEVEEAPEGEEGVNIGED 567

Query: 181 ---KKRKKGD-----KYRALLQSDEDGEQDGGQ---DMEVTFNTGL----EDISKRILEK 240
              KK +K D     KYR LLQ  ++ E+ G +   +ME+ +  GL    E++ K  LE 
Sbjct: 568 GKTKKSQKDDEEQIAKYRQLLQVIQEKEKKGKENDMEMEIKWVPGLKESAEEMVKNKLEG 627

Query: 241 KDKKSETLWEAHLRKKHEKRMASRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESG- 300
           KDK   T WE  L KK EK+   + + A + +D   +   +V+  D +F EE  VK+ G 
Sbjct: 628 KDKL--TPWEQFLEKKKEKKRLKKKQKALAEEDSEDELPSDVDFNDPYFAEE--VKKIGI 687

Query: 301 -KDRTKNIKGREHVGEDGAAEASRAELELLLADDD-------GVDTSIKGYNLKHKKKK- 360
            K   K+ K      E+   E  +AE+ LL+ D++         D  ++  NL  KKKK 
Sbjct: 688 KKKSMKSAKDSASSEEETDLEKQKAEMALLVMDEEEDSKKHFNYDKIVEHQNLSKKKKKQ 747

Query: 361 --GKEDITEDKIPTVDYNDPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGY 420
              K+++ ED    V+ +D RF A++ S LF LDP+DP FK++ A  + +  K       
Sbjct: 748 LMKKKELVEDDF-EVNVSDARFQAMYTSHLFNLDPSDPNFKKTKAMEKILEEK------- 807

Query: 421 QPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMKSKQLQ 445
              + R  K      A     +  G  + K   D +      LS L+KS+K K++Q Q
Sbjct: 808 --ARHRERKEELLIQAVERAQQDTGKPTQKQPMDPA------LSMLIKSVKNKTEQFQ 837

BLAST of Cucsat.G301 vs. ExPASy Swiss-Prot
Match: Q9H501 (ESF1 homolog OS=Homo sapiens OX=9606 GN=ESF1 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.0e-46
Identity = 168/478 (35.15%), Postives = 253/478 (52.93%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEE++ GPV L            +D  + +    EKLR Y+  RL+YYYAVV+CDS  T
Sbjct: 394 MKEEQVQGPVELL--------SIPEDAPEKDWTSREKLRDYQFKRLKYYYAVVDCDSPET 453

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAP-SSYEVLNFHTPALQHS 120
           A  +Y+ CDG+EFE S + +DLRFIPD + F+  P+D A+E   ++Y+   F + A+  S
Sbjct: 454 ASKIYEDCDGLEFESSCSFIDLRFIPDDITFDDEPKDVASEVNLTAYKPKYFTSAAMGTS 513

Query: 121 KIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLAS---DESESDDE--SDDG----ED 180
            + ++WDE + +R+  L RKF  ++L D++ + +LAS   DE E ++E   DDG    ED
Sbjct: 514 TVEITWDETDHERITMLNRKFKKEELLDMDFQAYLASSSEDEEEIEEELQGDDGVNVEED 573

Query: 181 QVDKKRKKGD-----KYRALLQSDEDGEQDGGQ---DMEVTFNTGL----EDISKRILEK 240
              KK +K D     KYR LLQ  ++ E+ G +   +ME+ +  GL    E++ K  LE 
Sbjct: 574 GKTKKSQKDDEEQIAKYRQLLQVIQEKEKKGKENDMEMEIKWVPGLKESAEEMVKNKLEG 633

Query: 241 KDKKSETLWEAHLRKKHEKRMASRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESG- 300
           KDK   T WE  L KK EK+   R + A + +    +   +V+  D +F EE  VK+ G 
Sbjct: 634 KDKL--TPWEQFLEKKKEKKRLKRKQKALAEEASEEELPSDVDLNDPYFAEE--VKQIGI 693

Query: 301 -KDRTKNIKGREHVGEDGAAEASRAELELLLADDD-------GVDTSIKGYNLKHKKKK- 360
            K   K+ K      E+   E  +AE+ LL+ D+D         +  ++  NL  KKKK 
Sbjct: 694 NKKSVKSAKDGTSPEEEIEIERQKAEMALLMMDEDEDSKKHFNYNKIVEHQNLSKKKKKQ 753

Query: 361 --GKEDITEDKIPTVDYNDPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGY 420
              K+++ ED    V+ ND RF A++ S LF LDP+DP FK++ A  + +  K  +    
Sbjct: 754 LMKKKELIEDDF-EVNVNDARFQAMYTSHLFNLDPSDPNFKKTKAMEKILEEKARQ---- 813

Query: 421 QPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMKSKQLQ 445
           +  K +    + K+  +  E ES+            K     LS L+KSIK K++Q Q
Sbjct: 814 RERKEQELTQAIKKKESEIEKESQ-----------RKSIDPALSMLIKSIKTKTEQFQ 843

BLAST of Cucsat.G301 vs. ExPASy Swiss-Prot
Match: O74828 (Pre-rRNA-processing protein esf1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=esf1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.2e-45
Identity = 156/457 (34.14%), Postives = 238/457 (52.08%), Query Frame = 0

Query: 13  FDGEQKKNDEDDDDDNDDEEMDNE----KLRAYEMSRLRYYYAVVECDSIATADYLYKTC 72
           F  +  ++D+D++D   +E++ NE    KLR Y++ RLRYYYAVVECDS+ TA  +Y+TC
Sbjct: 257 FGFDNNESDQDEEDALIEEDLGNEFDMVKLRQYQLERLRYYYAVVECDSVRTAKVIYETC 316

Query: 73  DGVEFERSSNILDLRFIPDSMKF-EHPPRDTATEAPSSYEVLNFHTPALQHSKIHLSWDE 132
           DG E+E S+NI DLRFIPD + F +   R+  T+AP  YE  +F T ALQHSK+ LSWD 
Sbjct: 317 DGAEYETSANIYDLRFIPDDVTFDDDESREVCTKAPEKYEPRDFVTDALQHSKVKLSWDA 376

Query: 133 DEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKK-----GDKY 192
           ++P R   +K+ F +  + DL+   ++AS ESE +D      D +  + +K      D +
Sbjct: 377 EDPHRKDLIKKAFTSQDIEDLDFSAYIASSESEDED-----VDVIRSRYQKLLSGDADDF 436

Query: 193 RALLQ--SDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 252
           +A      D+D  +    +MEVTF +G  D+         +K ET  E + RK  E++  
Sbjct: 437 QANSNPFEDDDKLEGANGEMEVTFTSGF-DVDNN--ANSSEKDETTIEKYKRKAAERKQR 496

Query: 253 SR--NKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEA 312
            +   +   + DDE   +D ++   D FF +    K++ ++  KN KG+    ED  A A
Sbjct: 497 RKELRQLKKTKDDEGEGSDVDLGFDDPFFKD----KDASRNNKKNKKGKHTQIEDPTA-A 556

Query: 313 SRAELELLLADDDGVDTSIKGYNLK--------------HKKKKGKEDITEDKIPTVDYN 372
           S+ ELE L+ +D+     +  +++K               KK    E + E      D +
Sbjct: 557 SKEELENLVREDENDSEQLDHFDMKSILKAEKFKKNRKLKKKASNLEGLQEGF--EADVS 616

Query: 373 DPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAP 432
           DPRF+AL+ +  FALDPT+P FKR+   V ++  +  K    Q  +++ GK   K     
Sbjct: 617 DPRFAALYTNHNFALDPTNPHFKRTKT-VEKIMDESRKRRSNQLEQTQDGKPELKIKKRK 676

Query: 433 GEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMKSK 442
            E   KGD            ++ EL  +VKSIK   K
Sbjct: 677 AE---KGD------------QRQELDRIVKSIKRSGK 682

BLAST of Cucsat.G301 vs. ExPASy Swiss-Prot
Match: Q756J5 (Pre-rRNA-processing protein ESF1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=ESF1 PE=3 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 2.0e-43
Identity = 147/477 (30.82%), Postives = 248/477 (51.99%), Query Frame = 0

Query: 1   MKEEELHGPV-GLFDGEQKKNDEDDDDD-----------NDDEEMDNEKLRAYEMSRLRY 60
           M+ EE+ GP   +F  ++ K  + DDDD           + +++ D++ LR Y++ RLRY
Sbjct: 197 MQREEVEGPPREVFKSKKDKKAKQDDDDEIGLKDLYEQGDAEKDYDSKALRRYQLERLRY 256

Query: 61  YYAVVECDSIATADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEV 120
           YYAVV C+++ATA+ +Y+ CDG E+E ++N+ DLR++P+ + F+  PR+     P  Y+ 
Sbjct: 257 YYAVVYCNNVATAEAIYQNCDGTEYESTANMFDLRYVPEGVTFDDEPREECASVPKDYKP 316

Query: 121 LNFHTPALQHSKIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDG 180
           + F T ALQHS++ L+WDE    RV+  KR F+  ++ D++ K +LASD  ES  E+DD 
Sbjct: 317 VQFSTSALQHSQVKLTWDETPADRVEMAKRAFSQKEIEDMDFKAYLASDSEES--EADDN 376

Query: 181 EDQVDKKRKKGDKYRAL---LQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSET 240
            +  +K R      +     L +DE  E++   D+++TF  GLE    +   ++D + E 
Sbjct: 377 SEAKNKLRSLVSSVKVADKPLFADESDEEEA--DVQITFTPGLEGGEAK---EEDAEEEN 436

Query: 241 LWEAHLRKKHEKRMASRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIK 300
           + E   RK+ E+R   + +                       V+E  +K+  ++  K+ K
Sbjct: 437 ILEKLKRKEKERRKKRKER-----------------------VKE--LKKQAEEEKKSSK 496

Query: 301 GREHVGEDGAAEAS----RAELELLLADDDGVDTSIKG---YN----LKHKKKKGKEDIT 360
             +H  +  + + S    RAELELL+ +DD    SI     +N    L+ +K++GK+   
Sbjct: 497 KEKHASKSESEQKSENDKRAELELLMMEDDENSQSINSSAHFNMNDILRSEKERGKKSKY 556

Query: 361 EDKIPTV------DYNDPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGYQP 420
           + K   V      D NDPRF  +F    FA+DP+ P+FK +AA ++Q+          Q 
Sbjct: 557 QKKDKIVEDDFKPDLNDPRFKEVFEDRDFAIDPSQPEFKETAA-MKQI---------LQE 616

Query: 421 TKSRHGKSSTKQPAAPGEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMKSKQLQL 446
            + R  KS++K+     + + + D ++ + GD        L  LV  +K K K+ +L
Sbjct: 617 RRKRSSKSASKK----RKTDEQSDSAMDSAGD--------LKGLVAKLKRKGKKPKL 619

BLAST of Cucsat.G301 vs. NCBI nr
Match: XP_004134297.1 (pre-rRNA-processing protein ESF1 [Cucumis sativus] >XP_011650670.1 pre-rRNA-processing protein ESF1 [Cucumis sativus] >KGN56410.1 hypothetical protein Csa_010247 [Cucumis sativus])

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 482/483 (99.79%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 258 MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 317

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK
Sbjct: 318 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 377

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD
Sbjct: 378 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 437

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA
Sbjct: 438 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 497

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR
Sbjct: 498 SRNKSADSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 557

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 558 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 617

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD
Sbjct: 618 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 677

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ 480
           SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ
Sbjct: 678 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ 737

Query: 481 RKM 483
           RKM
Sbjct: 738 RKM 740

BLAST of Cucsat.G301 vs. NCBI nr
Match: XP_008437867.1 (PREDICTED: pre-rRNA-processing protein esf1 [Cucumis melo] >TYK20826.1 pre-rRNA-processing protein esf1 [Cucumis melo var. makuwa])

HSP 1 Score: 854 bits (2207), Expect = 9.51e-307
Identity = 457/484 (94.42%), Postives = 464/484 (95.87%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQKKN EDDDDD   EEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQKKNVEDDDDD---EEMDNEKLRAYEMSRLRYYYAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPSSYEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSSYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGE+QVDKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGENQVDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKK EKRMA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKREKRMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDTDREV+EVDDFFVEEPPVKES KDR KNIKG+EHVG DGAAEASR
Sbjct: 496 SRNKSADSSDDESSDTDREVDEVDDFFVEEPPVKESAKDRIKNIKGKEHVGVDGAAEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYNDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQPTKS+HGKSSTKQPAAPGEDE+KGDV VKTEGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQPTKSQHGKSSTKQPAAPGEDEAKGDVPVKTEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKK- 480
           SSKKEKYELSSLVKSIKMKSKQLQL SG GKIPKKDRK +FP TEEELQPPTKNKS KK 
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGDGKIPKKDRKHRFPATEEELQPPTKNKSAKKK 735

Query: 481 QRKM 483
           QRKM
Sbjct: 736 QRKM 736

BLAST of Cucsat.G301 vs. NCBI nr
Match: KAA0048875.1 (pre-rRNA-processing protein esf1 [Cucumis melo var. makuwa])

HSP 1 Score: 847 bits (2188), Expect = 7.36e-304
Identity = 454/484 (93.80%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQKKN EDDDDD   EEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQKKNVEDDDDD---EEMDNEKLRAYEMSRLRYYYAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPSSYEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSSYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGE+QVDKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGENQVDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKK EK MA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKREKSMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDT REV+EVDDFFVEEPPVKES KDR KNIKG+EHVG DGAAEASR
Sbjct: 496 SRNKSADSSDDESSDTVREVDEVDDFFVEEPPVKESAKDRIKNIKGKEHVGVDGAAEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYNDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQPTKS+HGKSSTKQPAAPGEDE+KG+V VKTEGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQPTKSQHGKSSTKQPAAPGEDEAKGEVPVKTEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKK- 480
           SSKKEKYELSSLVKSIKMKSKQLQL SG GKIPKKDRK +FP TEEELQPPTKNKS KK 
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGDGKIPKKDRKHRFPATEEELQPPTKNKSAKKK 735

Query: 481 QRKM 483
           QRKM
Sbjct: 736 QRKM 736

BLAST of Cucsat.G301 vs. NCBI nr
Match: XP_038884339.1 (pre-rRNA-processing protein esf1 [Benincasa hispida])

HSP 1 Score: 801 bits (2070), Expect = 6.09e-286
Identity = 428/482 (88.80%), Postives = 449/482 (93.15%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQ+KNDEDDDD    EEMDNEKLRAYEMSRLRYY+AVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQEKNDEDDDDG---EEMDNEKLRAYEMSRLRYYFAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPS YEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSCYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDES+SDDESDDGE + DKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESDSDDESDDGE-RTDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDK SETLWEAHLRKK EKRMA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKISETLWEAHLRKKREKRMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           ++NKSA+SSDDE+SDTDREV+EVDDFFVEEPPVKES KDRTK+IK REHVG DG+ EASR
Sbjct: 496 AKNKSAHSSDDENSDTDREVDEVDDFFVEEPPVKESMKDRTKSIKDREHVGVDGSVEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADD+GVDT IKGYNLKHK+KKGKEDI EDKIPTVDY+DPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDEGVDTGIKGYNLKHKRKKGKEDIAEDKIPTVDYDDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQ TKS+HGKSSTKQPA  GEDE  GD  VK EGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQSTKSQHGKSSTKQPAVSGEDEVNGDAPVKIEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ 480
           SSKKEKYELSSLVKSIKMKSKQLQL SGGGK+ KKD K++FP  EEELQPPT NKS KK+
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGGGKMAKKDGKERFPAKEEELQPPTMNKSSKKK 733

Query: 481 RK 482
           ++
Sbjct: 736 QR 733

BLAST of Cucsat.G301 vs. NCBI nr
Match: XP_022926491.1 (pre-rRNA-processing protein esf1 [Cucurbita moschata])

HSP 1 Score: 747 bits (1929), Expect = 2.75e-264
Identity = 418/494 (84.62%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKND-EDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIA 60
           MKEEELHGPVGLFD EQ+KND +DDDDD+DDEE+DNEKLRAYEMSRLRYYYAVVECDSIA
Sbjct: 254 MKEEELHGPVGLFDDEQEKNDGDDDDDDDDDEEIDNEKLRAYEMSRLRYYYAVVECDSIA 313

Query: 61  TADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHS 120
           TADYLYKTCDGVEFERSSN+LDLRFIPDSM+F+HPPRDTATEAPSSYEVLNFHTPALQHS
Sbjct: 314 TADYLYKTCDGVEFERSSNVLDLRFIPDSMEFKHPPRDTATEAPSSYEVLNFHTPALQHS 373

Query: 121 KIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDES--DDG-EDQVDKKR 180
           KIHLSWDEDEPQRV ALKRKFNADQLADLELKEFLASDESES+DES  DDG EDQ DKKR
Sbjct: 374 KIHLSWDEDEPQRVTALKRKFNADQLADLELKEFLASDESESEDESGDDDGVEDQTDKKR 433

Query: 181 KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHE 240
           KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRK+ E
Sbjct: 434 KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKRRE 493

Query: 241 KRMASRNKSANSSDDESSDTDREV-EEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGA 300
           KR+ +R+KSA SSDD+SSDTDREV EE  DFFVEEPPVK+S KDRTKNIK REHVG DG 
Sbjct: 494 KRVNARSKSARSSDDDSSDTDREVGEEAGDFFVEEPPVKQSKKDRTKNIKDREHVGLDGE 553

Query: 301 AEASRAELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSP 360
           AEASRAELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDY+DPRFSALFNSP
Sbjct: 554 AEASRAELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYSDPRFSALFNSP 613

Query: 361 LFALDPTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSV 420
           L+ALDPTDPQFKRSAAYVRQVALKQ K DGYQ TKS+ G+SSTKQPAA G+D    +V V
Sbjct: 614 LYALDPTDPQFKRSAAYVRQVALKQQKVDGYQLTKSQLGESSTKQPAASGDDTVNSNVPV 673

Query: 421 KTEGDSSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTT-----EEELQP 480
            TEGDSS K KYELSSLVKSIKMKS+QLQL SGG K PK+D K +   T     E +L P
Sbjct: 674 MTEGDSSTK-KYELSSLVKSIKMKSQQLQLQSGGRKTPKEDGKKRPRATRATGEERQLPP 733

Query: 481 PTKNKSGKKQ-RKM 483
              NKSGKK+ RKM
Sbjct: 734 IMNNKSGKKKPRKM 746

BLAST of Cucsat.G301 vs. ExPASy TrEMBL
Match: A0A0A0L333 (NUC153 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119430 PE=3 SV=1)

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 482/483 (99.79%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 258 MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 317

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK
Sbjct: 318 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 377

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD
Sbjct: 378 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 437

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA
Sbjct: 438 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 497

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR
Sbjct: 498 SRNKSADSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 557

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 558 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 617

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD
Sbjct: 618 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 677

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ 480
           SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ
Sbjct: 678 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKKQ 737

Query: 481 RKM 483
           RKM
Sbjct: 738 RKM 740

BLAST of Cucsat.G301 vs. ExPASy TrEMBL
Match: A0A5D3DBA6 (Pre-rRNA-processing protein esf1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00880 PE=3 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 4.61e-307
Identity = 457/484 (94.42%), Postives = 464/484 (95.87%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQKKN EDDDDD   EEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQKKNVEDDDDD---EEMDNEKLRAYEMSRLRYYYAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPSSYEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSSYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGE+QVDKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGENQVDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKK EKRMA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKREKRMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDTDREV+EVDDFFVEEPPVKES KDR KNIKG+EHVG DGAAEASR
Sbjct: 496 SRNKSADSSDDESSDTDREVDEVDDFFVEEPPVKESAKDRIKNIKGKEHVGVDGAAEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYNDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQPTKS+HGKSSTKQPAAPGEDE+KGDV VKTEGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQPTKSQHGKSSTKQPAAPGEDEAKGDVPVKTEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKK- 480
           SSKKEKYELSSLVKSIKMKSKQLQL SG GKIPKKDRK +FP TEEELQPPTKNKS KK 
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGDGKIPKKDRKHRFPATEEELQPPTKNKSAKKK 735

Query: 481 QRKM 483
           QRKM
Sbjct: 736 QRKM 736

BLAST of Cucsat.G301 vs. ExPASy TrEMBL
Match: A0A1S3AUN8 (pre-rRNA-processing protein esf1 OS=Cucumis melo OX=3656 GN=LOC103483171 PE=3 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 4.61e-307
Identity = 457/484 (94.42%), Postives = 464/484 (95.87%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQKKN EDDDDD   EEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQKKNVEDDDDD---EEMDNEKLRAYEMSRLRYYYAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPSSYEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSSYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGE+QVDKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGENQVDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKK EKRMA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKREKRMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDTDREV+EVDDFFVEEPPVKES KDR KNIKG+EHVG DGAAEASR
Sbjct: 496 SRNKSADSSDDESSDTDREVDEVDDFFVEEPPVKESAKDRIKNIKGKEHVGVDGAAEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYNDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQPTKS+HGKSSTKQPAAPGEDE+KGDV VKTEGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQPTKSQHGKSSTKQPAAPGEDEAKGDVPVKTEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKK- 480
           SSKKEKYELSSLVKSIKMKSKQLQL SG GKIPKKDRK +FP TEEELQPPTKNKS KK 
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGDGKIPKKDRKHRFPATEEELQPPTKNKSAKKK 735

Query: 481 QRKM 483
           QRKM
Sbjct: 736 QRKM 736

BLAST of Cucsat.G301 vs. ExPASy TrEMBL
Match: A0A5A7U5G7 (Pre-rRNA-processing protein esf1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00900 PE=3 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 3.56e-304
Identity = 454/484 (93.80%), Postives = 462/484 (95.45%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKNDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIAT 60
           MKEEELHGPVGLFD EQKKN EDDDDD   EEMDNEKLRAYEMSRLRYYYAVVECDSIAT
Sbjct: 256 MKEEELHGPVGLFDDEQKKNVEDDDDD---EEMDNEKLRAYEMSRLRYYYAVVECDSIAT 315

Query: 61  ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHSK 120
           ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRD ATEAPSSYEVLNFHTPALQHSK
Sbjct: 316 ADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDIATEAPSSYEVLNFHTPALQHSK 375

Query: 121 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGEDQVDKKRKKGD 180
           IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGE+QVDKKRKKGD
Sbjct: 376 IHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGENQVDKKRKKGD 435

Query: 181 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHEKRMA 240
           KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKK EK MA
Sbjct: 436 KYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKREKSMA 495

Query: 241 SRNKSANSSDDESSDTDREVEEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGAAEASR 300
           SRNKSA+SSDDESSDT REV+EVDDFFVEEPPVKES KDR KNIKG+EHVG DGAAEASR
Sbjct: 496 SRNKSADSSDDESSDTVREVDEVDDFFVEEPPVKESAKDRIKNIKGKEHVGVDGAAEASR 555

Query: 301 AELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSPLFALD 360
           AELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDYNDPRFSALFNSPLFALD
Sbjct: 556 AELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYNDPRFSALFNSPLFALD 615

Query: 361 PTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSVKTEGD 420
           PTDPQFKRSAAYVRQVALKQ KGDGYQPTKS+HGKSSTKQPAAPGEDE+KG+V VKTEGD
Sbjct: 616 PTDPQFKRSAAYVRQVALKQQKGDGYQPTKSQHGKSSTKQPAAPGEDEAKGEVPVKTEGD 675

Query: 421 SSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTTEEELQPPTKNKSGKK- 480
           SSKKEKYELSSLVKSIKMKSKQLQL SG GKIPKKDRK +FP TEEELQPPTKNKS KK 
Sbjct: 676 SSKKEKYELSSLVKSIKMKSKQLQLQSGDGKIPKKDRKHRFPATEEELQPPTKNKSAKKK 735

Query: 481 QRKM 483
           QRKM
Sbjct: 736 QRKM 736

BLAST of Cucsat.G301 vs. ExPASy TrEMBL
Match: A0A6J1EF28 (pre-rRNA-processing protein esf1 OS=Cucurbita moschata OX=3662 GN=LOC111433624 PE=3 SV=1)

HSP 1 Score: 747 bits (1929), Expect = 1.33e-264
Identity = 418/494 (84.62%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKKND-EDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSIA 60
           MKEEELHGPVGLFD EQ+KND +DDDDD+DDEE+DNEKLRAYEMSRLRYYYAVVECDSIA
Sbjct: 254 MKEEELHGPVGLFDDEQEKNDGDDDDDDDDDEEIDNEKLRAYEMSRLRYYYAVVECDSIA 313

Query: 61  TADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQHS 120
           TADYLYKTCDGVEFERSSN+LDLRFIPDSM+F+HPPRDTATEAPSSYEVLNFHTPALQHS
Sbjct: 314 TADYLYKTCDGVEFERSSNVLDLRFIPDSMEFKHPPRDTATEAPSSYEVLNFHTPALQHS 373

Query: 121 KIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDES--DDG-EDQVDKKR 180
           KIHLSWDEDEPQRV ALKRKFNADQLADLELKEFLASDESES+DES  DDG EDQ DKKR
Sbjct: 374 KIHLSWDEDEPQRVTALKRKFNADQLADLELKEFLASDESESEDESGDDDGVEDQTDKKR 433

Query: 181 KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKKHE 240
           KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRK+ E
Sbjct: 434 KKGDKYRALLQSDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAHLRKRRE 493

Query: 241 KRMASRNKSANSSDDESSDTDREV-EEVDDFFVEEPPVKESGKDRTKNIKGREHVGEDGA 300
           KR+ +R+KSA SSDD+SSDTDREV EE  DFFVEEPPVK+S KDRTKNIK REHVG DG 
Sbjct: 494 KRVNARSKSARSSDDDSSDTDREVGEEAGDFFVEEPPVKQSKKDRTKNIKDREHVGLDGE 553

Query: 301 AEASRAELELLLADDDGVDTSIKGYNLKHKKKKGKEDITEDKIPTVDYNDPRFSALFNSP 360
           AEASRAELELLLADDDGVDT IKGYNLKHKKKKGKEDI EDKIPTVDY+DPRFSALFNSP
Sbjct: 554 AEASRAELELLLADDDGVDTGIKGYNLKHKKKKGKEDIAEDKIPTVDYSDPRFSALFNSP 613

Query: 361 LFALDPTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPAAPGEDESKGDVSV 420
           L+ALDPTDPQFKRSAAYVRQVALKQ K DGYQ TKS+ G+SSTKQPAA G+D    +V V
Sbjct: 614 LYALDPTDPQFKRSAAYVRQVALKQQKVDGYQLTKSQLGESSTKQPAASGDDTVNSNVPV 673

Query: 421 KTEGDSSKKEKYELSSLVKSIKMKSKQLQLPSGGGKIPKKDRKDQFPTT-----EEELQP 480
            TEGDSS K KYELSSLVKSIKMKS+QLQL SGG K PK+D K +   T     E +L P
Sbjct: 674 MTEGDSSTK-KYELSSLVKSIKMKSQQLQLQSGGRKTPKEDGKKRPRATRATGEERQLPP 733

Query: 481 PTKNKSGKKQ-RKM 483
              NKSGKK+ RKM
Sbjct: 734 IMNNKSGKKKPRKM 746

BLAST of Cucsat.G301 vs. TAIR 10
Match: AT3G01160.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cytosol, nucleus; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NUC153 (InterPro:IPR012580); Has 36638 Blast hits to 21323 proteins in 1057 species: Archae - 109; Bacteria - 2369; Metazoa - 13796; Fungi - 4858; Plants - 1657; Viruses - 489; Other Eukaryotes - 13360 (source: NCBI BLink). )

HSP 1 Score: 433.3 bits (1113), Expect = 2.5e-121
Identity = 256/457 (56.02%), Postives = 333/457 (72.87%), Query Frame = 0

Query: 1   MKEEELHGPVGLFDGEQKK--NDEDDDDDNDDEEMDNEKLRAYEMSRLRYYYAVVECDSI 60
           MKEEE+HGPV   DG++K   +D++D+++ +DE++ N+KLRAYE+SRL+YY+AV ECDS 
Sbjct: 246 MKEEEIHGPV--IDGDKKNDASDDEDEEEEEDEDVINQKLRAYEISRLKYYFAVAECDSS 305

Query: 61  ATADYLYKTCDGVEFERSSNILDLRFIPDSMKFEHPPRDTATEAPSSYEVLNFHTPALQH 120
           ATADYLYK+CDG+EFERSSN LDLRFIPDSM+F+HPPRD A+EAP+ YE L+F + ALQ 
Sbjct: 306 ATADYLYKSCDGIEFERSSNKLDLRFIPDSMEFKHPPRDIASEAPAGYEGLDFQSRALQM 365

Query: 121 SKIHLSWDEDEPQRVKALKRKFNADQLADLELKEFLASDESESDDESDDGED---QVDKK 180
           SK++LSWDEDEP R+K L +KFN +QLA+LE+KEFLASDES+SD+E D G +   Q  KK
Sbjct: 366 SKVNLSWDEDEPHRIKTLNQKFNPEQLANLEMKEFLASDESDSDEEDDLGNEVINQSKKK 425

Query: 181 RKKGDKYRALLQ-----SDEDGEQDGGQDMEVTFNTGLEDISKRILEKKDKKSETLWEAH 240
            KK DKYRAL++     SD+D E++  QDMEVTFNTGLED+SK IL+KKD +SE++WE +
Sbjct: 426 DKKKDKYRALIEAEDVDSDKDLEEENDQDMEVTFNTGLEDLSKEILKKKDNQSESVWETY 485

Query: 241 LRKKHEKRMASRNK----SANSSDDESSDTDREV---EEVDDFFVEEPPVKESGKDRTKN 300
           LR++ EK+ A +NK     ++  DD+  + DR+    +  DDFF+EEPP+K+  K+    
Sbjct: 486 LRQRREKKRARKNKQKDDDSSPDDDDDYNIDRKAVKDDGDDDFFMEEPPLKKKKKE---- 545

Query: 301 IKGREHVGEDGAAEASRAELELLLADDDGVD-TSIKGYNLKHKKKKGKEDITEDKIPTVD 360
             G+    E  A E SRAELELLLAD++  D   +KGYN+K K KKGK DI+EDKIP  +
Sbjct: 546 --GKTKKEEVAAEEKSRAELELLLADENAGDGNGLKGYNIKRKAKKGKTDISEDKIPAAE 605

Query: 361 YNDPRFSALFNSPLFALDPTDPQFKRSAAYVRQVALKQPKGDGYQPTKSRHGKSSTKQPA 420
            +DPRFSALF+SP +ALDPTDPQFKRSA Y RQ+ALKQ         +   G    K P 
Sbjct: 606 LDDPRFSALFSSPYYALDPTDPQFKRSATYARQLALKQ--------KEDPKGHEDVKAPK 665

Query: 421 APGEDESKGDVSVKTEGDSSKKEKYELSSLVKSIKMK 440
              E  S G++        SKKE++EL+S VKS+KMK
Sbjct: 666 EKQELNSDGNL-------GSKKERHELTSTVKSLKMK 679