Cucsat.G2580 (gene) Cucumber (B10) v3

BLAST of Cucsat.G2580 vs. ExPASy Swiss-Prot
Match: P29503 (Protein neuralized OS=Drosophila melanogaster OX=7227 GN=neur PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-09
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = 0

Query: 755 KCCICYSMEIDSLLYRCGHMCSCMKCGKELQWR---GGKCPVCRSPIEDVVQ 804
           +C ICY   IDS+LY CGHMC C  C  E QWR   GG+CP+CR+ I DV++
Sbjct: 700 ECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR 750

BLAST of Cucsat.G2580 vs. ExPASy Swiss-Prot
Match: Q24746 (Protein neuralized OS=Drosophila virilis OX=7244 GN=neur PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-09
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = 0

Query: 755 KCCICYSMEIDSLLYRCGHMCSCMKCGKELQWR---GGKCPVCRSPIEDVVQ 804
           +C ICY   IDS+LY CGHMC C  C  E QWR   GG+CP+CR+ I DV++
Sbjct: 693 ECTICYENPIDSVLYMCGHMCMCYDCAIE-QWRGVGGGQCPLCRAVIRDVIR 743

BLAST of Cucsat.G2580 vs. ExPASy Swiss-Prot
Match: Q0MW30 (E3 ubiquitin-protein ligase NEURL1B OS=Mus musculus OX=10090 GN=Neurl1b PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 2.1e-08
Identity = 24/54 (44.44%), Postives = 37/54 (68.52%), Query Frame = 0

Query: 751 SRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRG-GKCPVCRSPIEDVVQ 804
           SR  +C +C+  E+D+++Y CGHMC C  CG  L+ +    CP+CR PI+DV++
Sbjct: 489 SRNGECTVCFDSEVDTVIYTCGHMCLCHGCGLRLRRQARACCPICRRPIKDVIK 542

BLAST of Cucsat.G2580 vs. ExPASy Swiss-Prot
Match: A8MQ27 (E3 ubiquitin-protein ligase NEURL1B OS=Homo sapiens OX=9606 GN=NEURL1B PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.4e-07
Identity = 22/50 (44.00%), Postives = 35/50 (70.00%), Query Frame = 0

Query: 755 KCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRG-GKCPVCRSPIEDVVQ 804
           +C +C+  E+D+++Y CGHMC C  CG  L+ +    CP+CR PI+DV++
Sbjct: 502 ECTVCFDGEVDTVIYTCGHMCLCHSCGLRLKRQARACCPICRRPIKDVIK 551

BLAST of Cucsat.G2580 vs. ExPASy Swiss-Prot
Match: Q923S6 (E3 ubiquitin-protein ligase NEURL1 OS=Mus musculus OX=10090 GN=Neurl1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.0e-07
Identity = 22/50 (44.00%), Postives = 33/50 (66.00%), Query Frame = 0

Query: 755 KCCICYSMEIDSLLYRCGHMCSCMKCGKELQWR-GGKCPVCRSPIEDVVQ 804
           +C ICY   +D+++Y CGHMC C  CG  L+      CP+CR PI+D+++
Sbjct: 520 ECTICYEHAVDTVIYTCGHMCLCYSCGLRLKKALHACCPICRRPIKDIIK 569

BLAST of Cucsat.G2580 vs. NCBI nr
Match: XP_004134880.1 (uncharacterized protein LOC101206495 [Cucumis sativus] >KGN49544.1 hypothetical protein Csa_003486 [Cucumis sativus])

HSP 1 Score: 1566 bits (4056), Expect = 0.0
Identity = 810/810 (100.00%), Postives = 810/810 (100.00%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL
Sbjct: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD 180
           GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD
Sbjct: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD 180

Query: 181 AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW 240
           AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW
Sbjct: 181 AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW 240

Query: 241 VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD 300
           VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD
Sbjct: 241 VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD 300

Query: 301 ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER 360
           ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER
Sbjct: 301 ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER 360

Query: 361 FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM 420
           FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM
Sbjct: 361 FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM 420

Query: 421 HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS 480
           HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS
Sbjct: 421 HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS 480

Query: 481 QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN 540
           QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN
Sbjct: 481 QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN 540

Query: 541 DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE 600
           DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE
Sbjct: 541 DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE 600

Query: 601 GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN 660
           GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN
Sbjct: 601 GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN 660

Query: 661 RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS 720
           RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS
Sbjct: 661 RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS 720

Query: 721 IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC 780
           IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC
Sbjct: 721 IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC 780

Query: 781 GKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           GKELQWRGGKCPVCRSPIEDVVQASFSTRS
Sbjct: 781 GKELQWRGGKCPVCRSPIEDVVQASFSTRS 810

BLAST of Cucsat.G2580 vs. NCBI nr
Match: XP_008439420.1 (PREDICTED: uncharacterized protein LOC103484234 [Cucumis melo])

HSP 1 Score: 1456 bits (3769), Expect = 0.0
Identity = 764/812 (94.09%), Postives = 780/812 (96.06%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           N NPVL NFPVTKTNHDT+TKRSDDSQQH STNDPQTQTTSTP PPIPETS NKNQTSKL
Sbjct: 61  NTNPVLTNFPVTKTNHDTSTKRSDDSQQHASTNDPQTQTTSTPAPPIPETSPNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQ--ACSVEAGDFEDER 180
           GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQ  ACSVEAGDFEDER
Sbjct: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQEQACSVEAGDFEDER 180

Query: 181 YDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS 240
           YDAGPGSDDGFADWHSSRTSSSSPP STQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS
Sbjct: 181 YDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS 240

Query: 241 SWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRE 300
           SWVEDHNDQPNESSSLHPTLIP+DQVE KCLSHILCSPRIRGRQAFADLLLQIERDRQRE
Sbjct: 241 SWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGRQAFADLLLQIERDRQRE 300

Query: 301 LDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLR 360
           LD+LVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRS  IMHLR
Sbjct: 301 LDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSFTIMHLR 360

Query: 361 ERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVV 420
           ERFSGV ++GAKSPRGEMLN++DDDKTQ DTNANT T    PHVINTNEKDKDNDNQQVV
Sbjct: 361 ERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHT---PPHVINTNEKDKDNDNQQVV 420

Query: 421 GMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNS 480
           G+HSNNTD+DQI+GGFKEEQIEKQEQK   PDQELEPEQGGEVD PSLEGIWQDGSKLNS
Sbjct: 421 GIHSNNTDNDQIIGGFKEEQIEKQEQK---PDQELEPEQGGEVDPPSLEGIWQDGSKLNS 480

Query: 481 DSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS 540
           DS DSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS
Sbjct: 481 DSHDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS 540

Query: 541 ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCF 600
           ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQT QEEEYNEVKEDDDVDEELWCF
Sbjct: 541 ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYNEVKEDDDVDEELWCF 600

Query: 601 SEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGED 660
           SEGHTQ KSSDNEEEDDD ERSLI+AQYHEASDYLDQSASPLQLASPSILSSWSY+MGED
Sbjct: 601 SEGHTQPKSSDNEEEDDD-ERSLITAQYHEASDYLDQSASPLQLASPSILSSWSYDMGED 660

Query: 661 SNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELR 720
           SNRGTSISSPQPL PQFSSNNNQ RPSLVSASH+PSIEMELIYDLRGHMEQLYREMSELR
Sbjct: 661 SNRGTSISSPQPLLPQFSSNNNQHRPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELR 720

Query: 721 KSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCM 780
           KSIKCCMDMQLMLQHSIKR HEVGGRKSKKDKSRKPKCCICY+MEIDSLLYRCGHMCSCM
Sbjct: 721 KSIKCCMDMQLMLQHSIKRSHEVGGRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCM 780

Query: 781 KCGKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           KCGKELQWRGGKCPVCR+PIEDVVQAS S  S
Sbjct: 781 KCGKELQWRGGKCPVCRAPIEDVVQASLSAHS 805

BLAST of Cucsat.G2580 vs. NCBI nr
Match: KAA0052413.1 (altered inheritance of mitochondria protein 44 [Cucumis melo var. makuwa])

HSP 1 Score: 1450 bits (3754), Expect = 0.0
Identity = 764/818 (93.40%), Postives = 780/818 (95.35%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           N NPVL NFPVTKTNHDT+TKRSDDSQQH STNDPQTQTTSTP PPIPETS NKNQTSKL
Sbjct: 61  NTNPVLTNFPVTKTNHDTSTKRSDDSQQHASTNDPQTQTTSTPAPPIPETSPNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANAN------TAVCSTKQETEAEQEQ--ACSVEAG 180
           GASSLVQIWEKRLNVSS NIGLNANANAN      TAVCSTKQETEAEQEQ  ACSVEAG
Sbjct: 121 GASSLVQIWEKRLNVSS-NIGLNANANANANANANTAVCSTKQETEAEQEQEQACSVEAG 180

Query: 181 DFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTA 240
           DFEDERYDAGPGSDDGFADWHSSRTSSSSPP STQSQSSDAGERERVRVVDIIRRLTLTA
Sbjct: 181 DFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDIIRRLTLTA 240

Query: 241 AKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIE 300
           AKPPHSSWVEDHNDQPNESSSLHPTLIP+DQVE KCLSHILCSPRIRGRQAFADLLLQIE
Sbjct: 241 AKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGRQAFADLLLQIE 300

Query: 301 RDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSS 360
           RDRQRELD+LVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRS 
Sbjct: 301 RDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSF 360

Query: 361 NIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDN 420
            IMHLRERFSGV ++GAKSPRGEMLN++DDDKTQ DTNANT T    PHVINTNEKDKDN
Sbjct: 361 TIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHT---PPHVINTNEKDKDN 420

Query: 421 DNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQD 480
           DNQQVVG+HSNNTD+DQI+GGFKEEQIEKQEQK   PDQELEPEQGGEVD PSLEGIWQD
Sbjct: 421 DNQQVVGIHSNNTDNDQIIGGFKEEQIEKQEQK---PDQELEPEQGGEVDPPSLEGIWQD 480

Query: 481 GSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ 540
           GSKLNSDS DSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ
Sbjct: 481 GSKLNSDSHDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ 540

Query: 541 MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVD 600
           MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQT QEEEYNEVKEDDDVD
Sbjct: 541 MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYNEVKEDDDVD 600

Query: 601 EELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWS 660
           EELWCFSEGHTQ KSSDNEEEDDDDERSLI+AQYHEASDYLDQSASPLQLASPSILSSWS
Sbjct: 601 EELWCFSEGHTQPKSSDNEEEDDDDERSLITAQYHEASDYLDQSASPLQLASPSILSSWS 660

Query: 661 YEMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYR 720
           Y+MGEDSNRGTSISSPQPL PQFSSNNNQ RPSLVSASH+PSIEMELIYDLRGHMEQLYR
Sbjct: 661 YDMGEDSNRGTSISSPQPLLPQFSSNNNQHRPSLVSASHNPSIEMELIYDLRGHMEQLYR 720

Query: 721 EMSELRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCG 780
           EMSELRKSIKCCMDMQLMLQHSIKR HEVGGRKSKKDKSRKPKCCICY+MEIDSLLYRCG
Sbjct: 721 EMSELRKSIKCCMDMQLMLQHSIKRSHEVGGRKSKKDKSRKPKCCICYNMEIDSLLYRCG 780

Query: 781 HMCSCMKCGKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           HMCSCMKCGKELQWRGGKCPVCR+PIEDVVQAS S  S
Sbjct: 781 HMCSCMKCGKELQWRGGKCPVCRAPIEDVVQASLSAHS 811

BLAST of Cucsat.G2580 vs. NCBI nr
Match: XP_038877490.1 (putative uncharacterized protein DDB_G0271606 [Benincasa hispida])

HSP 1 Score: 1262 bits (3266), Expect = 0.0
Identity = 697/842 (82.78%), Postives = 745/842 (88.48%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVE SSSSSPFGCVLRDHNRRREP+V ATHVARFR+NLKTLVMDRLNDCITITPNK
Sbjct: 1   MASSQVEISSSSSPFGCVLRDHNRRREPNVAATHVARFRDNLKTLVMDRLNDCITITPNK 60

Query: 61  N----------PNPVLANFPVTKTNHDTTT----KRSDDSQQHTSTNDPQTQT-TSTPTP 120
           N          PNPVL NF V+KTNH+TTT    +R++DSQ+ TS NDPQTQT T T + 
Sbjct: 61  NKNKNQNQNQNPNPVLPNFRVSKTNHNTTTNAAPRRANDSQRQTSINDPQTQTQTQTTST 120

Query: 121 PIPETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANA-NTAVCSTKQETEAEQE 180
           P PET +NKNQTSKLGASSLVQIWEKRLNVSSSN+GLNANANA NTAVCS KQETE EQE
Sbjct: 121 PTPETGTNKNQTSKLGASSLVQIWEKRLNVSSSNVGLNANANASNTAVCSAKQETETEQE 180

Query: 181 QACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDI 240
           QACSVE GDFEDERYDAGPGS+DGFADWHSSRTSSSSPP STQSQSSDAGERERVRVVDI
Sbjct: 181 QACSVEVGDFEDERYDAGPGSEDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDI 240

Query: 241 IRRLTLTAAKPPHSSWVEDHNDQPNESSSL-HPTLIPRDQVE-SKCLSHILCSPRIRGRQ 300
           IRRLTLTAAKPPHSSWVEDHND PNESSS  HPTLI RDQVE ++CLSHILCSPRIRGRQ
Sbjct: 241 IRRLTLTAAKPPHSSWVEDHNDHPNESSSSSHPTLILRDQVEPARCLSHILCSPRIRGRQ 300

Query: 301 AFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFV 360
           AFADLLLQIERDRQRELDILVERRAVSKF QRGRIQSLLRLKILKRGMALEDEQKRPQFV
Sbjct: 301 AFADLLLQIERDRQRELDILVERRAVSKFSQRGRIQSLLRLKILKRGMALEDEQKRPQFV 360

Query: 361 ITPRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHV 420
           ITPRENHRSS IMHLRE+FSGV ++GA+SP GEMLNN+D+DK QS TNA+T      PH 
Sbjct: 361 ITPRENHRSSTIMHLREKFSGVGENGARSPIGEMLNNNDEDKNQSHTNAHTP-----PHA 420

Query: 421 INTNEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQ-----QKPDQELEP-- 480
            NTNEK+KDNDN+QVVG+HSN+ ++DQIL GFKEEQIE++E++Q     Q+ +QE EP  
Sbjct: 421 TNTNEKEKDNDNEQVVGIHSNSINNDQILEGFKEEQIEEREREQEQEQKQEIEQEQEPKQ 480

Query: 481 --EQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFA 540
             EQG EVD PS EG WQD   LN DSQDS+NGWEAE QSEA EESYGADYVGTSYDWF+
Sbjct: 481 EQEQGEEVDPPSSEGTWQDRPNLNLDSQDSINGWEAEDQSEAAEESYGADYVGTSYDWFS 540

Query: 541 DISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLE 600
           DISRPRSYWEDRRQSWYQQMLDS+SANDEIRQLIQRKTVS FLSSDFRERMDKLMVTRLE
Sbjct: 541 DISRPRSYWEDRRQSWYQQMLDSSSANDEIRQLIQRKTVSTFLSSDFRERMDKLMVTRLE 600

Query: 601 RQTHQEEEYNEVKEDDDVD--EELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASD 660
           RQT +EEEY+EV E+DD D  EELWCFSEG TQSKSSDN EE++D ERSLISAQYHEASD
Sbjct: 601 RQTQEEEEYDEVNEEDDDDRAEELWCFSEGRTQSKSSDNGEEEED-ERSLISAQYHEASD 660

Query: 661 YLDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLV 720
           YLDQS SPLQLASPSILSSWSY    EMGEDSNRG S SSPQP QPQFSSNN QQ  SLV
Sbjct: 661 YLDQSTSPLQLASPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSNN-QQGSSLV 720

Query: 721 SASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGG-RKS 780
           S +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR HEVGG R+S
Sbjct: 721 STTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR-HEVGGGRRS 780

Query: 781 KKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRSPIEDVVQASF 808
           KK+KSRKPKCCICY+MEIDSLLYRCGHMCSCMKC KELQWRGGKCPVCR+PIEDVVQASF
Sbjct: 781 KKEKSRKPKCCICYNMEIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCRAPIEDVVQASF 834

BLAST of Cucsat.G2580 vs. NCBI nr
Match: XP_022925581.1 (trichohyalin-like [Cucurbita moschata] >KAG7034825.1 Protein neuralized, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 630/859 (73.34%), Postives = 696/859 (81.02%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASS+VE SSS+SPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  N----PNPVLANFPVTKTNHDTTTKRSDDSQ----------QHTSTNDPQTQTTSTPTPP 120
           N    PNPV+ NF V +TNHD+  +RS+  Q           + + N+PQT+TT     P
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSAPRRSNPCQTLSTIINHPQNNNNNNNPQTRTT-----P 120

Query: 121 IPETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANT--AVCSTKQETEAEQE 180
            P+T ++KN +SKLGASSLVQIWEKRLNVSSSN+GLNANANAN   +VCS KQETE EQE
Sbjct: 121 TPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQETEQEQE 180

Query: 181 Q--------ACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGER 240
           Q        ACS+EAGDF DERYDAG GS+D FADWHSSRTSSSSPP STQSQ SDA ER
Sbjct: 181 QEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQSQISDARER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSP 300
           ERVRVVDIIRRLTLTAAKPPHSSWVED ND  NESSS +PTLI R QVE KCLSHIL SP
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVED-NDHSNESSS-NPTLILRYQVEPKCLSHILYSP 300

Query: 301 RIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360
           RIRGRQAFADLLLQIERDRQREL+ LVERRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQ
Sbjct: 301 RIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQ 360

Query: 361 KRPQFVITPRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTH 420
           KRP+FVITPR NHR+  I HLRERFSG  ++GA+SP GEML+N+DDDK Q DT+ +T   
Sbjct: 361 KRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDPHT--- 420

Query: 421 SHTPHVINT----NEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQE 480
               H  NT    N+ DKD++NQQVVG++        I   F EE+IE   +K+++P QE
Sbjct: 421 ----HATNTKDNDNDNDKDSNNQQVVGINP-------IPEHFNEEEIE---EKEEEPAQE 480

Query: 481 LEPEQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWF 540
            EPEQ  EVD PS EG WQD   LN DSQDS+NGWEAE  SEA EESY  +Y+GTSYDWF
Sbjct: 481 PEPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESYDENYLGTSYDWF 540

Query: 541 ADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRL 600
           ADISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLI+RKTVSNFLSS+FRERMDKLMV+RL
Sbjct: 541 ADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSRL 600

Query: 601 ERQTHQEEEYN---------EVKEDDDVDEELWCFSEGHTQSKSSDNEEEDDD-----DE 660
           ERQT QEEEY+         E +E+++ +EELWCFSEGHTQ KSSDNEEE+++     DE
Sbjct: 601 ERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPKSSDNEEEEEEEEEDRDE 660

Query: 661 RSLIS-AQYHEASDYLDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQP 720
           RSLIS AQY EASD LD SASPLQ  SPSILSSWSY    EMGEDSNRG S SSPQP QP
Sbjct: 661 RSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQP 720

Query: 721 QFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQH 780
           QFSSN   QR S VS +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQH
Sbjct: 721 QFSSNT--QRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQH 780

Query: 781 SIKRVHEVGG--RKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKC 810
           SIKR HEVGG  RKSKK+KSRK KCCICY M+IDSLLYRCGHMCSCMKC KELQWRGGKC
Sbjct: 781 SIKR-HEVGGGGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKC 832

BLAST of Cucsat.G2580 vs. ExPASy TrEMBL
Match: A0A0A0KL44 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G538730 PE=4 SV=1)

HSP 1 Score: 1566 bits (4056), Expect = 0.0
Identity = 810/810 (100.00%), Postives = 810/810 (100.00%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL
Sbjct: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD 180
           GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD
Sbjct: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYD 180

Query: 181 AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW 240
           AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW
Sbjct: 181 AGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSW 240

Query: 241 VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD 300
           VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD
Sbjct: 241 VEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELD 300

Query: 301 ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER 360
           ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER
Sbjct: 301 ILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLRER 360

Query: 361 FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM 420
           FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM
Sbjct: 361 FSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGM 420

Query: 421 HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS 480
           HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS
Sbjct: 421 HSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNSDS 480

Query: 481 QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN 540
           QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN
Sbjct: 481 QDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNSAN 540

Query: 541 DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE 600
           DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE
Sbjct: 541 DEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSE 600

Query: 601 GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN 660
           GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN
Sbjct: 601 GHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSN 660

Query: 661 RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS 720
           RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS
Sbjct: 661 RGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKS 720

Query: 721 IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC 780
           IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC
Sbjct: 721 IKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKC 780

Query: 781 GKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           GKELQWRGGKCPVCRSPIEDVVQASFSTRS
Sbjct: 781 GKELQWRGGKCPVCRSPIEDVVQASFSTRS 810

BLAST of Cucsat.G2580 vs. ExPASy TrEMBL
Match: A0A1S3AYB9 (uncharacterized protein LOC103484234 OS=Cucumis melo OX=3656 GN=LOC103484234 PE=4 SV=1)

HSP 1 Score: 1456 bits (3769), Expect = 0.0
Identity = 764/812 (94.09%), Postives = 780/812 (96.06%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           N NPVL NFPVTKTNHDT+TKRSDDSQQH STNDPQTQTTSTP PPIPETS NKNQTSKL
Sbjct: 61  NTNPVLTNFPVTKTNHDTSTKRSDDSQQHASTNDPQTQTTSTPAPPIPETSPNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQ--ACSVEAGDFEDER 180
           GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQ  ACSVEAGDFEDER
Sbjct: 121 GASSLVQIWEKRLNVSSSNIGLNANANANTAVCSTKQETEAEQEQEQACSVEAGDFEDER 180

Query: 181 YDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS 240
           YDAGPGSDDGFADWHSSRTSSSSPP STQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS
Sbjct: 181 YDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHS 240

Query: 241 SWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRE 300
           SWVEDHNDQPNESSSLHPTLIP+DQVE KCLSHILCSPRIRGRQAFADLLLQIERDRQRE
Sbjct: 241 SWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGRQAFADLLLQIERDRQRE 300

Query: 301 LDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSSNIMHLR 360
           LD+LVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRS  IMHLR
Sbjct: 301 LDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSFTIMHLR 360

Query: 361 ERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVV 420
           ERFSGV ++GAKSPRGEMLN++DDDKTQ DTNANT T    PHVINTNEKDKDNDNQQVV
Sbjct: 361 ERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHT---PPHVINTNEKDKDNDNQQVV 420

Query: 421 GMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQDGSKLNS 480
           G+HSNNTD+DQI+GGFKEEQIEKQEQK   PDQELEPEQGGEVD PSLEGIWQDGSKLNS
Sbjct: 421 GIHSNNTDNDQIIGGFKEEQIEKQEQK---PDQELEPEQGGEVDPPSLEGIWQDGSKLNS 480

Query: 481 DSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS 540
           DS DSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS
Sbjct: 481 DSHDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSNS 540

Query: 541 ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCF 600
           ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQT QEEEYNEVKEDDDVDEELWCF
Sbjct: 541 ANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYNEVKEDDDVDEELWCF 600

Query: 601 SEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGED 660
           SEGHTQ KSSDNEEEDDD ERSLI+AQYHEASDYLDQSASPLQLASPSILSSWSY+MGED
Sbjct: 601 SEGHTQPKSSDNEEEDDD-ERSLITAQYHEASDYLDQSASPLQLASPSILSSWSYDMGED 660

Query: 661 SNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELR 720
           SNRGTSISSPQPL PQFSSNNNQ RPSLVSASH+PSIEMELIYDLRGHMEQLYREMSELR
Sbjct: 661 SNRGTSISSPQPLLPQFSSNNNQHRPSLVSASHNPSIEMELIYDLRGHMEQLYREMSELR 720

Query: 721 KSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCM 780
           KSIKCCMDMQLMLQHSIKR HEVGGRKSKKDKSRKPKCCICY+MEIDSLLYRCGHMCSCM
Sbjct: 721 KSIKCCMDMQLMLQHSIKRSHEVGGRKSKKDKSRKPKCCICYNMEIDSLLYRCGHMCSCM 780

Query: 781 KCGKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           KCGKELQWRGGKCPVCR+PIEDVVQAS S  S
Sbjct: 781 KCGKELQWRGGKCPVCRAPIEDVVQASLSAHS 805

BLAST of Cucsat.G2580 vs. ExPASy TrEMBL
Match: A0A5A7U932 (Altered inheritance of mitochondria protein 44 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G00080 PE=4 SV=1)

HSP 1 Score: 1450 bits (3754), Expect = 0.0
Identity = 764/818 (93.40%), Postives = 780/818 (95.35%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNQ 60

Query: 61  NPNPVLANFPVTKTNHDTTTKRSDDSQQHTSTNDPQTQTTSTPTPPIPETSSNKNQTSKL 120
           N NPVL NFPVTKTNHDT+TKRSDDSQQH STNDPQTQTTSTP PPIPETS NKNQTSKL
Sbjct: 61  NTNPVLTNFPVTKTNHDTSTKRSDDSQQHASTNDPQTQTTSTPAPPIPETSPNKNQTSKL 120

Query: 121 GASSLVQIWEKRLNVSSSNIGLNANANAN------TAVCSTKQETEAEQEQ--ACSVEAG 180
           GASSLVQIWEKRLNVSS NIGLNANANAN      TAVCSTKQETEAEQEQ  ACSVEAG
Sbjct: 121 GASSLVQIWEKRLNVSS-NIGLNANANANANANANTAVCSTKQETEAEQEQEQACSVEAG 180

Query: 181 DFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTA 240
           DFEDERYDAGPGSDDGFADWHSSRTSSSSPP STQSQSSDAGERERVRVVDIIRRLTLTA
Sbjct: 181 DFEDERYDAGPGSDDGFADWHSSRTSSSSPPSSTQSQSSDAGERERVRVVDIIRRLTLTA 240

Query: 241 AKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIE 300
           AKPPHSSWVEDHNDQPNESSSLHPTLIP+DQVE KCLSHILCSPRIRGRQAFADLLLQIE
Sbjct: 241 AKPPHSSWVEDHNDQPNESSSLHPTLIPKDQVEPKCLSHILCSPRIRGRQAFADLLLQIE 300

Query: 301 RDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSS 360
           RDRQRELD+LVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRS 
Sbjct: 301 RDRQRELDVLVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHRSF 360

Query: 361 NIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDN 420
            IMHLRERFSGV ++GAKSPRGEMLN++DDDKTQ DTNANT T    PHVINTNEKDKDN
Sbjct: 361 TIMHLRERFSGVGENGAKSPRGEMLNDNDDDKTQPDTNANTHT---PPHVINTNEKDKDN 420

Query: 421 DNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPSLEGIWQD 480
           DNQQVVG+HSNNTD+DQI+GGFKEEQIEKQEQK   PDQELEPEQGGEVD PSLEGIWQD
Sbjct: 421 DNQQVVGIHSNNTDNDQIIGGFKEEQIEKQEQK---PDQELEPEQGGEVDPPSLEGIWQD 480

Query: 481 GSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ 540
           GSKLNSDS DSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ
Sbjct: 481 GSKLNSDSHDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQ 540

Query: 541 MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVD 600
           MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQT QEEEYNEVKEDDDVD
Sbjct: 541 MLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTQQEEEYNEVKEDDDVD 600

Query: 601 EELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWS 660
           EELWCFSEGHTQ KSSDNEEEDDDDERSLI+AQYHEASDYLDQSASPLQLASPSILSSWS
Sbjct: 601 EELWCFSEGHTQPKSSDNEEEDDDDERSLITAQYHEASDYLDQSASPLQLASPSILSSWS 660

Query: 661 YEMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYR 720
           Y+MGEDSNRGTSISSPQPL PQFSSNNNQ RPSLVSASH+PSIEMELIYDLRGHMEQLYR
Sbjct: 661 YDMGEDSNRGTSISSPQPLLPQFSSNNNQHRPSLVSASHNPSIEMELIYDLRGHMEQLYR 720

Query: 721 EMSELRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCG 780
           EMSELRKSIKCCMDMQLMLQHSIKR HEVGGRKSKKDKSRKPKCCICY+MEIDSLLYRCG
Sbjct: 721 EMSELRKSIKCCMDMQLMLQHSIKRSHEVGGRKSKKDKSRKPKCCICYNMEIDSLLYRCG 780

Query: 781 HMCSCMKCGKELQWRGGKCPVCRSPIEDVVQASFSTRS 810
           HMCSCMKCGKELQWRGGKCPVCR+PIEDVVQAS S  S
Sbjct: 781 HMCSCMKCGKELQWRGGKCPVCRAPIEDVVQASLSAHS 811

BLAST of Cucsat.G2580 vs. ExPASy TrEMBL
Match: A0A6J1ECL3 (trichohyalin-like OS=Cucurbita moschata OX=3662 GN=LOC111432973 PE=4 SV=1)

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 630/859 (73.34%), Postives = 696/859 (81.02%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASS+VE SSS+SPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  N----PNPVLANFPVTKTNHDTTTKRSDDSQ----------QHTSTNDPQTQTTSTPTPP 120
           N    PNPV+ NF V +TNHD+  +RS+  Q           + + N+PQT+TT     P
Sbjct: 61  NQNHNPNPVIPNFRVPRTNHDSAPRRSNPCQTLSTIINHPQNNNNNNNPQTRTT-----P 120

Query: 121 IPETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANT--AVCSTKQETEAEQE 180
            P+T ++KN +SKLGASSLVQIWEKRLNVSSSN+GLNANANAN   +VCS KQETE EQE
Sbjct: 121 TPQTGTDKNHSSKLGASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQETEQEQE 180

Query: 181 Q--------ACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGER 240
           Q        ACS+EAGDF DERYDAG GS+D FADWHSSRTSSSSPP STQSQ SDA ER
Sbjct: 181 QEQEQEQEQACSLEAGDFGDERYDAGLGSEDVFADWHSSRTSSSSPPSSTQSQISDARER 240

Query: 241 ERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSP 300
           ERVRVVDIIRRLTLTAAKPPHSSWVED ND  NESSS +PTLI R QVE KCLSHIL SP
Sbjct: 241 ERVRVVDIIRRLTLTAAKPPHSSWVED-NDHSNESSS-NPTLILRYQVEPKCLSHILYSP 300

Query: 301 RIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQ 360
           RIRGRQAFADLLLQIERDRQREL+ LVERRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQ
Sbjct: 301 RIRGRQAFADLLLQIERDRQRELETLVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQ 360

Query: 361 KRPQFVITPRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTH 420
           KRP+FVITPR NHR+  I HLRERFSG  ++GA+SP GEML+N+DDDK Q DT+ +T   
Sbjct: 361 KRPKFVITPRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDPHT--- 420

Query: 421 SHTPHVINT----NEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQE 480
               H  NT    N+ DKD++NQQVVG++        I   F EE+IE   +K+++P QE
Sbjct: 421 ----HATNTKDNDNDNDKDSNNQQVVGINP-------IPEHFNEEEIE---EKEEEPAQE 480

Query: 481 LEPEQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWF 540
            EPEQ  EVD PS EG WQD   LN DSQDS+NGWEAE  SEA EESY  +Y+GTSYDWF
Sbjct: 481 PEPEQEQEVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESYDENYLGTSYDWF 540

Query: 541 ADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRL 600
           ADISRPRSYWEDRR+SWYQQMLDSNSAN+EIRQLI+RKTVSNFLSS+FRERMDKLMV+RL
Sbjct: 541 ADISRPRSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSRL 600

Query: 601 ERQTHQEEEYN---------EVKEDDDVDEELWCFSEGHTQSKSSDNEEEDDD-----DE 660
           ERQT QEEEY+         E +E+++ +EELWCFSEGHTQ KSSDNEEE+++     DE
Sbjct: 601 ERQTQQEEEYDDGAEEEDEEEEEEEEEGEEELWCFSEGHTQPKSSDNEEEEEEEEEDRDE 660

Query: 661 RSLIS-AQYHEASDYLDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQP 720
           RSLIS AQY EASD LD SASPLQ  SPSILSSWSY    EMGEDSNRG S SSPQP QP
Sbjct: 661 RSLISSAQYQEASDDLDPSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQP 720

Query: 721 QFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQH 780
           QFSSN   QR S VS +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQH
Sbjct: 721 QFSSNT--QRSSPVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQH 780

Query: 781 SIKRVHEVGG--RKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKC 810
           SIKR HEVGG  RKSKK+KSRK KCCICY M+IDSLLYRCGHMCSCMKC KELQWRGGKC
Sbjct: 781 SIKR-HEVGGGGRKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKC 832

BLAST of Cucsat.G2580 vs. ExPASy TrEMBL
Match: A0A6J1IHV7 (uncharacterized protein LOC111477598 OS=Cucurbita maxima OX=3661 GN=LOC111477598 PE=4 SV=1)

HSP 1 Score: 1090 bits (2819), Expect = 0.0
Identity = 627/847 (74.03%), Postives = 689/847 (81.35%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNK 60
           MASS+VE SSS+SPFGCVLRDHNRRREP+VTATHVARFRNNLKTLVMDRLNDCITITPN+
Sbjct: 1   MASSEVEISSSASPFGCVLRDHNRRREPNVTATHVARFRNNLKTLVMDRLNDCITITPNR 60

Query: 61  N----PNPVLANFPVTKTNHDTTTKRSDDSQ----------QHTSTNDPQTQTTSTPTPP 120
           N    PNPV+ NF   +TNHD+  +RS+  Q           + + N+PQ +TT     P
Sbjct: 61  NQNHNPNPVIPNFRGPRTNHDSAPRRSNPCQTLSTIINHPQNNNNNNNPQIRTT-----P 120

Query: 121 IPETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANT--AVCSTKQETEAEQE 180
            PET ++KN +SKL ASSLVQIWEKRLNVSSSN+GLNANANAN   +VCS KQETE EQE
Sbjct: 121 TPETGTDKNHSSKL-ASSLVQIWEKRLNVSSSNVGLNANANANATPSVCSVKQETEQEQE 180

Query: 181 QACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVRVVDI 240
           QACS+EAGDF DERYDAG GS+DGFADWHSSRTSSSSPP STQSQ SDA ERERVRVVDI
Sbjct: 181 QACSLEAGDFSDERYDAGLGSEDGFADWHSSRTSSSSPPSSTQSQISDARERERVRVVDI 240

Query: 241 IRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKCLSHILCSPRIRGRQAF 300
           IRRLTLTAAKPPHSSWVED ND  +ESSS +PTLI R QVE KCLSHIL SPRIRGRQAF
Sbjct: 241 IRRLTLTAAKPPHSSWVED-NDHSSESSS-NPTLILRYQVEPKCLSHILYSPRIRGRQAF 300

Query: 301 ADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMALEDEQKRPQFVIT 360
           ADLLLQIERDRQREL+ LVERRAVSKFPQRGRIQSLLRLKIL+RGMALEDEQKRP+FVIT
Sbjct: 301 ADLLLQIERDRQRELEALVERRAVSKFPQRGRIQSLLRLKILQRGMALEDEQKRPKFVIT 360

Query: 361 PRENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVIN 420
           PR NHR+  I HLRERFSG  ++GA+SP GEML+N+DDDK Q DT+A+T       H  N
Sbjct: 361 PRANHRAYTISHLRERFSGAGENGARSPIGEMLDNNDDDKNQLDTDAHT-------HATN 420

Query: 421 TNEKDKDNDN------QQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQG 480
            N+ D DNDN      QQVVG++        I   F EE+IE+QE     P QE  PE  
Sbjct: 421 ANDNDNDNDNDKDSNNQQVVGINP-------IPEDFNEEEIEEQE-----PVQEPVPEP- 480

Query: 481 GEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRP 540
            EVD PS EG WQD   LN DSQDS+NGWEAE  SEA EESY  +Y+GTSYDWFADISRP
Sbjct: 481 -EVDPPSSEGRWQDRPNLNLDSQDSINGWEAEDHSEAAEESYDENYLGTSYDWFADISRP 540

Query: 541 RSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQ 600
           RSYWEDRR+SWYQQMLDSNSAN+EIRQLI+RKTVSNFLSS+FRERMDKLMV+RLERQT Q
Sbjct: 541 RSYWEDRRKSWYQQMLDSNSANEEIRQLIERKTVSNFLSSEFRERMDKLMVSRLERQTQQ 600

Query: 601 EEEYN-----EVKEDDDVDEELWCFSEGHTQSKSSDNEEEDDD---DERSLIS-AQYHEA 660
           EEEY+     E +E+++ +EELWCFSEGHTQ +SSDNEEE+++   DERSLIS A Y EA
Sbjct: 601 EEEYDDGAEEEEEEEEEEEEELWCFSEGHTQPESSDNEEEEEEEDRDERSLISSAPYQEA 660

Query: 661 SDYLDQSASPLQLASPSILSSWSY----EMGEDSNRGTSISSPQPLQPQFSSNNNQQRPS 720
           SD LDQSASPLQ  SPSILSSWSY    EMGEDSNRG S SSPQP QPQFSSN   QR S
Sbjct: 661 SDDLDQSASPLQFPSPSILSSWSYQLDNEMGEDSNRGASTSSPQPFQPQFSSNT--QRSS 720

Query: 721 LVSASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGG-- 780
            VS +HHPSIEMELIYDLRGHMEQLY+EMSELRKSIKCCMDMQLMLQHSIKR HEVGG  
Sbjct: 721 PVSTTHHPSIEMELIYDLRGHMEQLYQEMSELRKSIKCCMDMQLMLQHSIKR-HEVGGGG 780

Query: 781 RKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCSCMKCGKELQWRGGKCPVCRSPIEDVVQ 810
           RKSKK+KSRK KCCICY M+IDSLLYRCGHMCSCMKC KELQWRGGKCPVC +PIEDVV+
Sbjct: 781 RKSKKEKSRKRKCCICYDMQIDSLLYRCGHMCSCMKCAKELQWRGGKCPVCGAPIEDVVR 815

BLAST of Cucsat.G2580 vs. TAIR 10
Match: AT1G30860.1 (RING/U-box superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 1.2e-59
Identity = 230/765 (30.07%), Postives = 364/765 (47.58%), Query Frame = 0

Query: 94  DPQTQTTSTPTPPIPETSSNKNQTSKLGASSLVQIWEKRLNVSSSNIGLNANANANTAVC 153
           D   +T +     + E+     +   L ASSLVQIWE RLN   SN G + +++ +TA  
Sbjct: 45  DSWIETHNNKNNRVLESPLESKRNDNLVASSLVQIWEARLN--RSNGGNSPSSSQSTASS 104

Query: 154 STKQETEAEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDA 213
           S++ ++    + +   E+   +          D   AD      S S          SD+
Sbjct: 105 SSRSDSGVSVQDSGLSESSSID---------GDSEIADRTVEIESRS------HGLVSDS 164

Query: 214 GERERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPR-----DQVESKC 273
           GE +  RV D+IRRL+    K          ++       L     PR        E   
Sbjct: 165 GESKWGRVADLIRRLSNEDKKRTAG------DNGSGGGGGLEIIRTPRPYCTSSSSEKSN 224

Query: 274 LSHILCSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKR 333
              +  SP+IRGRQAF D L+Q+ERDR RELD L ER AVS+F QRGR+QS+LRL+ L R
Sbjct: 225 FPVVSFSPKIRGRQAFTDFLMQMERDRHRELDSLFERNAVSRFTQRGRLQSMLRLRNLNR 284

Query: 334 GMALEDEQKRPQFVITPRENHRSSNIMHLRERF--------SGVDKHGAKSPRGEMLNND 393
            + ++D  +              S ++HLRE+F        +  DK      R   +NN 
Sbjct: 285 CLVIQDRNRSNAKTTGLNRIESGSAVLHLREKFHANVVNSAAATDKR-KNHQRSTEMNNK 344

Query: 394 DDDKTQSDTNANTQ------------THSHTPHVINTNEKDKDNDNQQVVGMHSNNTDDD 453
             ++T+  T  +T+                 P      E     + + V G   +  +  
Sbjct: 345 SVEETEGTTTTSTRLKDGGVTLEAFFAERLRPRNRKIEEATLCKEEESVSGTVDSQKNCL 404

Query: 454 QILGGFKEEQI------EKQEQKQQKPDQELEPEQG----------GEV--DLPSLEGIW 513
           Q+    + E+       EK+E++   P + L  E            G+   +    EG+ 
Sbjct: 405 QLQETREVEETCNDGKSEKKEEENTSPSEYLSRESRLRQNQDENNVGKYMQETRETEGLV 464

Query: 514 QDGSKLNS--DSQDS--MNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRR 573
            + ++++   D Q++  +N W  E +  A E+SY  +Y   + DW ++I+RPRSYWE+ R
Sbjct: 465 HESNEMDQCLDQQETSYLNRW-GEQEEYADEQSYYGEY---NDDWLSEIARPRSYWEELR 524

Query: 574 QSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEVK 633
           +S Y +++++ S  D+I +L++R+TV++FL S  RE++D LM++R+  QTH ++   + +
Sbjct: 525 KSRYLEVMNTRSEKDDICRLLERRTVTDFLDSGLREKIDNLMMSRV--QTHSDKHSKKWE 584

Query: 634 EDDDVDEEL-WCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASP 693
              + +EE+ +   E   +      EE+DD D+ S               S+S +  +SP
Sbjct: 585 LQQEEEEEVNFEIDEEIKEEPLRGGEEQDDRDDLS-------------QSSSSHISASSP 644

Query: 694 SILSSWSYEMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRG 753
           +   SW       S++ T ++S   L  Q     N Q P           EMELI  +R 
Sbjct: 645 A--GSW-------SSQDTDVTSTPALVVQ-----NPQSP-----------EMELISGMRS 704

Query: 754 HMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEID 811
            ++QL +EMS LR S+K C+D    LQH           K+ ++   K KCC+C   +++
Sbjct: 705 QIQQLQQEMSVLRDSVKTCLDANASLQH-----------KAHQENPMKRKCCVCDETQVE 730

BLAST of Cucsat.G2580 vs. TAIR 10
Match: AT2G34920.1 (RING/U-box superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 5.0e-58
Identity = 249/814 (30.59%), Postives = 379/814 (46.56%), Query Frame = 0

Query: 15  FGCVLRDHNRRREPSVTATHVARFRNNLKTLVMDRLNDCITITPNKNPNPVLANFPVTKT 74
           FG VLRD N+R    V       F+ NLK  V             K   P +++      
Sbjct: 8   FGSVLRDRNQRHNDDVV------FKKNLKAQV-------------KTAPPAISD------ 67

Query: 75  NHDTTTKRSDDSQQHTSTNDPQTQTTSTP-TPPIPETSSNKNQTSKL-GASSLVQIWEKR 134
                 + S++       N  +    S P +P  P T    N +  L GASSLVQIWE R
Sbjct: 68  ------ESSENRVDSLIGNKRKKNNKSRPGSPEKPRTRKGNNFSDNLGGASSLVQIWEAR 127

Query: 135 LNVSSSNIGLNANANANTAVCSTKQETEAEQEQACSVEAGDFEDERYDAGPGSDDGFADW 194
           LN S+           N+A+ S   E  +E     SV+      E +   P S DG ++ 
Sbjct: 128 LNRSN---------GGNSAIHSQSIEISSE----ASVQ------EIHLLAP-SIDGESE- 187

Query: 195 HSSRTSSSSPPCSTQSQSSDAGERERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESS 254
             S   S SP  + + +S          V DIIRRL+            E      N   
Sbjct: 188 --SENESKSPDQTVEIESGTLNS-----VSDIIRRLS-----------NEQKLTASNNGG 247

Query: 255 SLHPTLIPRDQVESKCLSHILCSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFP 314
           ++   ++    +E      + CSPRIRGRQA++DLL+ +ER+R REL+ L+ R AVS+FP
Sbjct: 248 AVDMPIVKTPTLEKSSFQVVTCSPRIRGRQAYSDLLVHLERERHRELESLLGRNAVSRFP 307

Query: 315 QRGRIQSLLRLKILKRGMALEDEQKRPQFVITPRENHR---SSNIMHLRERFSGVDKHGA 374
           QRGR+QS+LRL+ LKRG+A++D  +      T  + +R   SS I+HLRE+      + A
Sbjct: 308 QRGRLQSMLRLRSLKRGLAIQDRHRG----TTKSDLNRFQPSSTILHLREKLREKAANAA 367

Query: 375 KSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGMHSNNTDDD- 434
            +  G         +T+S  +  T    H+P   +T        N +   +  N T+   
Sbjct: 368 -AEAGLKKGQQSTVETESMQSKETSGILHSP---STERLSPQKRNIEEAILRKNETETKM 427

Query: 435 ---QILGGFKEEQIEKQEQK--------QQKPDQELEPEQGGEVDLPSLEGIWQDGSKLN 494
              Q+      E +E++            Q+P + L  E+ G+++    EG  Q+   L 
Sbjct: 428 SYLQLKKAIVAEVLERESDNTSPLTSVTPQEP-RILRNEEAGKLE-SGTEGT-QETPFLE 487

Query: 495 SDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDRRQSWYQQMLDSN 554
           +      +GWE + + E  E+SY   Y   SYDWF +ISRPR+YWED R+S Y +++++ 
Sbjct: 488 TQEMSFQSGWEEQEEYE-DEQSY---YGDMSYDWFTEISRPRTYWEDLRKSRYLEVMNTK 547

Query: 555 SANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLE-RQTHQEEEYNEVKEDDDVDEELW 614
           S  D+I +L++R+TVS FL S  RE++DKL+++R++    H+ EE  + +E  D+ EE  
Sbjct: 548 SDKDDICRLLERRTVSGFLQSGLREKIDKLIMSRVQIHPAHRIEEATKEEEKYDIGEE-- 607

Query: 615 CFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMG 674
                         ++ED DD               L QS+S  Q+ +PS   SW     
Sbjct: 608 --------------KDEDRDD---------------LSQSSS--QIFAPSPAGSW----- 652

Query: 675 EDSNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSE 734
             S+  T ++S                    + +H+    ++L             EMSE
Sbjct: 668 --SSLDTGVTS--------------------TPTHNLHSTLQL-------------EMSE 652

Query: 735 LRKSIKCCMDMQLMLQHSIKRVHEVGGRKSKKDKSRKPKCCICYSMEIDSLLYRCGHMCS 794
           LR S+K C+D+   LQ S+             +   K KCC+C   ++++LLYRCGHMC+
Sbjct: 728 LRDSVKTCLDVNASLQKSV-----------HLENPFKRKCCVCNETQVETLLYRCGHMCT 652

Query: 795 CMKCGKELQWRGGKCPVCRSPIEDVVQASFSTRS 811
           C++C  ELQ+ GGKCP+C + I DVV+    +R+
Sbjct: 788 CLRCANELQYNGGKCPICHAKILDVVRVFVDSRT 652

BLAST of Cucsat.G2580 vs. TAIR 10
Match: AT4G20160.1 (LOCATED IN: chloroplast; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G30860.1); Has 64917 Blast hits to 39974 proteins in 1949 species: Archae - 279; Bacteria - 7425; Metazoa - 26604; Fungi - 5788; Plants - 3193; Viruses - 275; Other Eukaryotes - 21353 (source: NCBI BLink). )

HSP 1 Score: 143.7 bits (361), Expect = 6.6e-34
Identity = 135/433 (31.18%), Postives = 210/433 (48.50%), Query Frame = 0

Query: 346  RENHRSSNIMHLRERFSGVDKHGAKSPRGEMLNNDDDDKTQSDTNANTQTHSHTPHVINT 405
            RE  +  N +   E+   +    AKS + E+  N   + +Q + +A+        H    
Sbjct: 807  REKDKERNSLQYGEKMCFLRNSEAKSTK-EIERNKSQEVSQGEESAS--------HGSRE 866

Query: 406  NEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQKQQKPDQELEPEQGGEVDLPS 465
            + K+K++  Q        N +D +   G KE + E+ ++ +++                 
Sbjct: 867  SAKEKNSSQQDDETSTHRNPNDKK---GIKEPEDEESKKVERE----------------- 926

Query: 466  LEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYGADYVGTSYDWFADISRPRSYWEDR 525
                 + G  +   S + +N W+     E  EE YG DY     DW  DISRPRSYWED 
Sbjct: 927  -----ETGENVEEASVEFVNDWDGNEMEEEEEEEYG-DYFNGDDDWIHDISRPRSYWEDL 986

Query: 526  RQSWYQQMLDSNSANDEIRQLIQRKTVSNFLSSDFRERMDKLMVTRLERQTHQEEEYNEV 585
            R+  Y ++L++ S   +I  LI+R+TVSNFL+SD R+++D LM+TR+  Q+H     N++
Sbjct: 987  RKERYLEVLNTESEKKDICNLIERRTVSNFLTSDLRQKIDNLMITRV--QSHIGVPLNQI 1046

Query: 586  KEDDDVDEELWCFSEGHTQSKSSDNEEEDDDDERSLISAQYHEASDYLDQSASPLQLASP 645
            +E D+ +EE W       + + S   EED++ E           SD   QS+     A  
Sbjct: 1047 EEGDEYEEE-W-------EVECSARNEEDNETEEEPEKTNLEAPSDVCSQSS-----ARS 1106

Query: 646  SILSSWSY---EMGEDSNRGTSISSPQPLQPQFSSNNNQQRPSLVSASHHPSIEMELIYD 705
            S + SW++   ++ +D+   TS+S P+PL P   +N + Q             +M+ I D
Sbjct: 1107 STMMSWNFRDQDIDKDNEPTTSLSLPEPLVP---TNQSTQ-------------DMQTISD 1166

Query: 706  LRGHMEQLYREMSELRKSIKCCMDMQLMLQHSIKRVHEVGG-----RKSKKDKSRKPKCC 765
            L+  MEQL REM ELR ++K C+DMQL  Q S+ +     G     R   K    K KCC
Sbjct: 1167 LKEQMEQLQREMLELRNTVKSCIDMQLHFQKSVTQDLSRSGSSAEQRVDPKKDPLKRKCC 1173

Query: 766  ICYSMEIDSLLYR 771
            +C  M +DSLLYR
Sbjct: 1227 VCSEMPVDSLLYR 1173

HSP 2 Score: 90.9 bits (224), Expect = 5.1e-18
Identity = 139/500 (27.80%), Postives = 220/500 (44.00%), Query Frame = 0

Query: 1   MASSQVEFSSSSSPFGCVL---------RDHN-------RRREPSVTATHVARFRNNLKT 60
           MASS+VE SS  + F CVL         RD+N            +V   HVA F  NL  
Sbjct: 1   MASSEVEMSSCQT-FNCVLSRPHERCTQRDNNTNNNKKKNNNNNNVRNIHVAAFEKNLNV 60

Query: 61  LVMDRLNDCITITPNKNPNPVLANFPVTKTN----HDTTTKRSDDSQQHTSTNDPQTQTT 120
            V D L +C +++ +   +  +   P   +N     D    ++++S+Q   ++     + 
Sbjct: 61  FVRDHLENC-SVSVDDVVDDSIKAVPECSSNKSSVSDHRLSKTEESRQDVPSSSSSLGSD 120

Query: 121 STPTPPIPETSSNKNQTSKLGASSLVQIWEKR------------LNVSSSNIGLNANANA 180
           S P    PE  S         ASSLVQIWE R            ++  +S++G NA  N+
Sbjct: 121 SDPNSGQPENPSRN------AASSLVQIWEARTTQQPPSSNQSLIDSRTSSMGSNALENS 180

Query: 181 NTAVCSTKQETEAEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQS 240
             ++ S K+    +  + C+ E    E+E  +  P                  PP     
Sbjct: 181 E-SLESVKESQMIQPIEECNNEE---EEEEIECAP------------------PPL---- 240

Query: 241 QSSDAGERERVRVVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVESKC 300
             S   +RE VRV+DIIR+L+  +    ++      ND   E  +           E++ 
Sbjct: 241 -ESGEKDREGVRVMDIIRKLSNDSETITNNDNGSSGNDNSKEVQT----------TEARS 300

Query: 301 LSHILCSPRIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKR 360
              + CSPRIRGRQA ADLL+Q+ RDR+++L  L ER  VSKF  RGRIQS LR++  +R
Sbjct: 301 FPQVACSPRIRGRQALADLLVQMTRDREKDLACLRERHCVSKFTNRGRIQSTLRIRCYER 360

Query: 361 GMALEDEQKRPQFVITPRENH--RSSNIMH-LRERFSGVDKH---GAK----SPRGEMLN 420
            +A++   +          N   R S +MH LRE++    ++   GA     S RG +++
Sbjct: 361 CLAIQGRHRSKSTSAGSDSNRSSRGSGVMHLLREKYKANSENIETGASTSHASTRGRIMD 420

Query: 421 NDDDDKTQSDTNANTQTHSHTPHVINTNEKDKDNDNQQVVGMHSNNTDDDQILGG--FKE 457
            D    T+      T   S     I       +N  + V+   S N +  + L G   ++
Sbjct: 421 KDPHKATEKKVLQETIEKSGVKE-IKEVVSAVENAKKGVLSEISENRNGLKKLAGETIQK 454

BLAST of Cucsat.G2580 vs. TAIR 10
Match: AT5G04460.1 (RING/U-box superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 1.1e-31
Identity = 186/685 (27.15%), Postives = 313/685 (45.69%), Query Frame = 0

Query: 161 AEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVR 220
           + +EQ+  +  G+ E  R+ A    D    D HSS         + + +     ERERVR
Sbjct: 234 SSREQSPDLGDGERERVRHIARGWMDSRIND-HSSNVRQRDD--NRRPEWLGDTERERVR 293

Query: 221 VVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVE-SKCLSHILCSP--- 280
           ++    ++T        ++  ED     +E+   H      D+V     ++H    P   
Sbjct: 294 IIREWMQMTSQQRGGARATPREDQR-STSEADRNHDAAPQVDRVRVGLAVNHEEGQPPHV 353

Query: 281 -----RIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMA 340
                R+RGRQA  DLL++ ER+RQREL  L+E RAVS F  R RIQSLLR + L+    
Sbjct: 354 RRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHRNRIQSLLRGRFLRNERP 413

Query: 341 LEDEQKRPQFVITPRENHRSSNIMHLRER--FSGVDK---HGAKSPRGEMLNNDDDDKTQ 400
              E+       TP  +  S  ++ LRER   SG+ +   +G ++   E  +N D+D + 
Sbjct: 414 TVPER-------TP--SMASRELLQLRERQTVSGLREGFHNGRENIVHENTSNTDNDNSN 473

Query: 401 SDTN--ANTQTHSHTPHVINTNEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQ 460
           + TN  A   T  ++  V + +       N   +   ++ ++       ++E+  +++  
Sbjct: 474 TSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESNLANADRDWEEDTNQRRIW 533

Query: 461 KQQKPDQE---LEPEQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYG 520
           ++  P  E   LE     + D      I +D + ++   +++    + EY++   +E++G
Sbjct: 534 QENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREASGFADDEYRT---QEAHG 593

Query: 521 ADYVGTSY----DW---FADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSN 580
             +  +S     +W    ++  R R   + RR + +    D N  + E+R+L+ R++VSN
Sbjct: 594 VWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNVYSMELRELLSRRSVSN 653

Query: 581 FLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSEGHTQSKSSDNEEED 640
            L S FRE +D+L+ +  ER+ H             VD +L      + Q+   D+ E D
Sbjct: 654 LLHSGFRESLDQLIQSYAERRGHTH-----------VDWDL----HANLQTAIPDSPERD 713

Query: 641 DDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSNRGTSISSPQPLQPQ 700
            D +  +     ++ +        P   A P                      PQP+  Q
Sbjct: 714 TDHQ--VFVRNDNQLNGINGSQLLPTPPAPP----------------------PQPIWHQ 773

Query: 701 FSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHS 760
              + +  R S+    H   IE E++ DLRG + +L + MS++++ ++ CMDMQL LQ S
Sbjct: 774 DLHHTSWSRHSM----HRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEACMDMQLELQRS 833

Query: 761 IKRVHEVGGRKSKKDKS---------------RKPKCCICYSMEIDSLLYRCGHMCSCMK 805
           +++       +S  D+                 K  CC+C    ID+LLYRCGHMC+C K
Sbjct: 834 VRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDALLYRCGHMCTCSK 859

BLAST of Cucsat.G2580 vs. TAIR 10
Match: AT5G04460.3 (RING/U-box superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 1.1e-31
Identity = 186/685 (27.15%), Postives = 313/685 (45.69%), Query Frame = 0

Query: 161 AEQEQACSVEAGDFEDERYDAGPGSDDGFADWHSSRTSSSSPPCSTQSQSSDAGERERVR 220
           + +EQ+  +  G+ E  R+ A    D    D HSS         + + +     ERERVR
Sbjct: 234 SSREQSPDLGDGERERVRHIARGWMDSRIND-HSSNVRQRDD--NRRPEWLGDTERERVR 293

Query: 221 VVDIIRRLTLTAAKPPHSSWVEDHNDQPNESSSLHPTLIPRDQVE-SKCLSHILCSP--- 280
           ++    ++T        ++  ED     +E+   H      D+V     ++H    P   
Sbjct: 294 IIREWMQMTSQQRGGARATPREDQR-STSEADRNHDAAPQVDRVRVGLAVNHEEGQPPHV 353

Query: 281 -----RIRGRQAFADLLLQIERDRQRELDILVERRAVSKFPQRGRIQSLLRLKILKRGMA 340
                R+RGRQA  DLL++ ER+RQREL  L+E RAVS F  R RIQSLLR + L+    
Sbjct: 354 RRDLRRVRGRQALLDLLMRAERERQRELQGLLEHRAVSDFAHRNRIQSLLRGRFLRNERP 413

Query: 341 LEDEQKRPQFVITPRENHRSSNIMHLRER--FSGVDK---HGAKSPRGEMLNNDDDDKTQ 400
              E+       TP  +  S  ++ LRER   SG+ +   +G ++   E  +N D+D + 
Sbjct: 414 TVPER-------TP--SMASRELLQLRERQTVSGLREGFHNGRENIVHENTSNTDNDNSN 473

Query: 401 SDTN--ANTQTHSHTPHVINTNEKDKDNDNQQVVGMHSNNTDDDQILGGFKEEQIEKQEQ 460
           + TN  A   T  ++  V + +       N   +   ++ ++       ++E+  +++  
Sbjct: 474 TSTNALAIAITAGNSQRVTDESSTSSRQGNDSPILPDNSESNLANADRDWEEDTNQRRIW 533

Query: 461 KQQKPDQE---LEPEQGGEVDLPSLEGIWQDGSKLNSDSQDSMNGWEAEYQSEAGEESYG 520
           ++  P  E   LE     + D      I +D + ++   +++    + EY++   +E++G
Sbjct: 534 QENVPVDERPNLEQTTLTQFDGYDNTDINRDETSVSDMHREASGFADDEYRT---QEAHG 593

Query: 521 ADYVGTSY----DW---FADISRPRSYWEDRRQSWYQQMLDSNSANDEIRQLIQRKTVSN 580
             +  +S     +W    ++  R R   + RR + +    D N  + E+R+L+ R++VSN
Sbjct: 594 VWHENSSRQSDGNWPGTRSEALRSRRVVQLRRLNRFHPPEDDNVYSMELRELLSRRSVSN 653

Query: 581 FLSSDFRERMDKLMVTRLERQTHQEEEYNEVKEDDDVDEELWCFSEGHTQSKSSDNEEED 640
            L S FRE +D+L+ +  ER+ H             VD +L      + Q+   D+ E D
Sbjct: 654 LLHSGFRESLDQLIQSYAERRGHTH-----------VDWDL----HANLQTAIPDSPERD 713

Query: 641 DDDERSLISAQYHEASDYLDQSASPLQLASPSILSSWSYEMGEDSNRGTSISSPQPLQPQ 700
            D +  +     ++ +        P   A P                      PQP+  Q
Sbjct: 714 TDHQ--VFVRNDNQLNGINGSQLLPTPPAPP----------------------PQPIWHQ 773

Query: 701 FSSNNNQQRPSLVSASHHPSIEMELIYDLRGHMEQLYREMSELRKSIKCCMDMQLMLQHS 760
              + +  R S+    H   IE E++ DLRG + +L + MS++++ ++ CMDMQL LQ S
Sbjct: 774 DLHHTSWSRHSM----HRSEIEWEVMNDLRGDVARLQQGMSQMQRMLEACMDMQLELQRS 833

Query: 761 IKRVHEVGGRKSKKDKS---------------RKPKCCICYSMEIDSLLYRCGHMCSCMK 805
           +++       +S  D+                 K  CC+C    ID+LLYRCGHMC+C K
Sbjct: 834 VRQEVSAALNRSAGDQGMSAETSEDGSRWSHVSKGTCCVCCDNHIDALLYRCGHMCTCSK 859