Cucsat.G2334 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G2334
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Location	ctg1002: 3711504 .. 3713828 (+)
RNA-Seq Expression	Cucsat.G2334
Synteny	Cucsat.G2334

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GACAGAGCAGACTTTGCCGATCTTTCTCTCGCGCGAGTCAGCCTTCTATTCTTTCTGCTTTTCTACTTTCAGAGGTCGAGCCCACGGAGCGAGCGGTCAGAATATCTCAACTGGAAAGCAAGGGGTAAAGAAGTTCAGATCGGGAAGAGTCCCCGCCGATTGTGGAGGAAGCGATAAACAATTTGTATTGTTCGAGTTTTCCTACTAGTTTGGCAATAGCTGATTTGGGATGTTCTTCGGGACCAAACGCTTTGATGGCAGCTTCTGAACTGATTAAAGCAGTGGAAATAATTCGTCAAAAACTGAAGAAGAAACCAATTGAATATCAAGTATTGTTAAATGACTTACCGGGAAATGACTTCAATACAATCTTCAAATCATTGCCTAATTTCCTTCAAAATTTGAGAAGGGAAATTGGAGGTGATGTTGGGCCTTGTCTTTTTACTGGAGTGCCTGCTTCTTTTTATGGAAGGCTTTTTCCTAAGAAAAGTGTCCATTTTGTTCATTCTTCTTACAGTCTTCATTGGCTATCCAAGGTAAATATTAACTTTTCTTCTTCTTCCTTTTTTCTCATCATCAAAGATTGGTAGAGAGCGTAAAACACTTTTTTTCAAAAAAAAAAAAAAAATTAAAGGTGATTACGAGTTGGGTAGATCGAGAGAAGTTTCTGTGTATTACATACATTATATACAAAATGCATGTGAACATATACTTCTCTAGCTATAATTGATTCGATACATAATAGTAAATTCATACTATTAATTTTTTTATTGTGTGTAAGAGGAGAAACCTTAGTAGTGGTTAATTGATTCACTTTTGTTTGAATATGACTAATTTTAGATTTTATTTGCTCATTTTAGCCCTGTATTTTTAAAAAAATTATTTTAGTTTCTGAACTTCTAACAAAAAATATTTTTCATTTTTGCTAGATTCTACTTCTTTTTCCTTTAATATTCTGTTGTTAAATGCTTGTCCCTAAGCATCATCCATATATTAAATTTTGAGTAATTTCCCTAAGGGAAAAAAGTACATAGAAATATCATTAGTTTTAAAAGAAAGAAAGAAAAAAAAAACAATAAAAGCAATAACCAAAATAACAGTTTTATTGGTTTAAAGATTAACTAGCGAAAATAGAAAATGGAAACCCTAAACCTTTGAGATGCCTAAATAGGTGCTCTACTAATCCTTTTTCCTCTAGGATCTTATTCAAAAAATGAAAAAAAAATGGCAGTTTAGTGACAAAATGAATATATTAAATATTTAAAGACTAAAAGAAAAACAAGTCTAAAAATTGATGAAACAAAAATGAGATTTAAACCATAGTTTTCTATTGTTCTTGTGTATAAGAGTATTGAAATTTGCAAAAAAAGCATGTACATAGATTTCTTAGAGGGTATTTGGACCCTTAACTTGAAGTTGGTTAAGTGGAGCTGTTATAACTTCGCTGAGCTCCATATATAATTATGATGCAGTGAATCTATATCATTTAAAACTTAGTCAACTGAATCTCCCAAATTAATGCCCCACCTTCTCTAACTTTGCAAGGATTGAAATTTGTGTAGGTTCCTGAAGGGTTGGAGGAGAATAAAAGAAACATATACATGACTGGTAATAGCCCCAGGAGTGTTGTGAAGGCCTATTACAATCAATTCCAAAAGGACTTTTCATTATTTCTCAAGTGTCGTGCACAAGAATTGGTGGATGGTGGTCGTATGATTTTGACATTGCTAGGAAGGAGAAGCCAAAACCCAGCTAGCAAAGAATGCTCTTACATTTGGGAGCTTTTGGGTCTAGCTCTTAACGACCTAGTCGATCAGGTAAACACCATCGTGTCTAATCTCATATTTATCTTAATGTGGTATCACAACAATAGAACCCTCAAATAATAATTACAACAATTGTAAGATACACAAGTTACTTTCGATCTTTTCCAGGGAATCATAGAAGAGGAGAAATTGGAGAGTTTCCACATACCCAAATACATGCCATCACCAATAGAAATAAGGATTGAGGTTGCAAAAGAAGCATCTTTTGTGATTGATAGCATTAAAGTTTCACAAGTAGATTGGAACGTTTCGGATAATAATGAAATGAACAAAGCAAAATCAGTAGATGTAAGTTTGAAGGGGAGTGGATACAATGTAGCAAAGTACATGAGGGCAGTGGCTGAACCAATTCTTATCAGTCATTTTGGAGAAGAAGTTATGGATGAATTGTTCATCAGATACAGAGAAATCATTGCAGACCGTATGGCCAAAGAGACAACTCAATTTTTCAATGTCACTGTTTCCCTTACTAAACCAAGGTAATTATGGAAAATGACCT

Coding sequence (CDS)

ATGGCAGCTTCTGAACTGATTAAAGCAGTGGAAATAATTCGTCAAAAACTGAAGAAGAAACCAATTGAATATCAAGTATTGTTAAATGACTTACCGGGAAATGACTTCAATACAATCTTCAAATCATTGCCTAATTTCCTTCAAAATTTGAGAAGGGAAATTGGAGGTGATGTTGGGCCTTGTCTTTTTACTGGAGTGCCTGCTTCTTTTTATGGAAGGCTTTTTCCTAAGAAAAGTGTCCATTTTGTTCATTCTTCTTACAGTCTTCATTGGCTATCCAAGGTTCCTGAAGGGTTGGAGGAGAATAAAAGAAACATATACATGACTGGTAATAGCCCCAGGAGTGTTGTGAAGGCCTATTACAATCAATTCCAAAAGGACTTTTCATTATTTCTCAAGTGTCGTGCACAAGAATTGGTGGATGGTGGTCGTATGATTTTGACATTGCTAGGAAGGAGAAGCCAAAACCCAGCTAGCAAAGAATGCTCTTACATTTGGGAGCTTTTGGGTCTAGCTCTTAACGACCTAGTCGATCAGGGAATCATAGAAGAGGAGAAATTGGAGAGTTTCCACATACCCAAATACATGCCATCACCAATAGAAATAAGGATTGAGGTTGCAAAAGAAGCATCTTTTGTGATTGATAGCATTAAAGTTTCACAAGTAGATTGGAACGTTTCGGATAATAATGAAATGAACAAAGCAAAATCAGTAGATGTAAGTTTGAAGGGGAGTGGATACAATGTAGCAAAGTACATGAGGGCAGTGGCTGAACCAATTCTTATCAGTCATTTTGGAGAAGAAGTTATGGATGAATTGTTCATCAGATACAGAGAAATCATTGCAGACCGTATGGCCAAAGAGACAACTCAATTTTTCAATGTCACTGTTTCCCTTACTAAACCAAGGTAA

Protein sequence

MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGPCLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAYYNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQGIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDVSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLTKPR

Homology

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BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot
Match: Q84UB4 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2 OS=Petunia hybrida OX=4102 GN=BSMT2 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 9.3e-95
Identity = 170/301 (56.48%), Postives = 223/301 (74.09%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +  S+L+K VE  R+K   K  E+    NDLPGNDFNT+F+SL  F ++LR+ IG   GP
Sbjct: 67  LVVSQLVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGP 126

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           C F+GVP SFY RLFP KS+HFV+SSYSL WLS+VP G+E NK NIYM   SP SV+KAY
Sbjct: 127 CFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAY 186

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           Y Q++ DFS FLK R++EL+ GG+M+LTLLGR S++P SKEC YIWELL +ALN LV++G
Sbjct: 187 YKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEG 246

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           +I+EEK+++F+IP+Y PSP E++  V KE SF I+ ++ S+V WN S+N           
Sbjct: 247 LIKEEKVDAFNIPQYTPSPAEVKYIVEKEGSFTINRLETSRVHWNASNNE---------- 306

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
             K  GYNV++ MRAVAEP+L+SHF +E+MD +F +Y EII+D M+KE T+F NV VSLT
Sbjct: 307 --KNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIISDCMSKEKTEFINVIVSLT 355

Query: 301 K 302
           K
Sbjct: 367 K 355

[Close]

BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot
Match: Q84UB5 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1 OS=Petunia hybrida OX=4102 GN=BSMT1 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 168/301 (55.81%), Postives = 223/301 (74.09%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +  S+L+K VE  R+K   K  E+    NDLPGNDFNT+F+SL  F ++LR+ IG   GP
Sbjct: 67  LVVSQLVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGP 126

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           C F+GVP SFY RLFP KS+HFV+SSYSL WLS+VP G+E NK NIYM   SP SV+KAY
Sbjct: 127 CFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAY 186

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           Y Q++ DFS FLK R++EL+ GG+M+LTLLGR S++P SKEC YIWELL +ALN LV++G
Sbjct: 187 YKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEG 246

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           +I+EEK+++F+IP+Y PSP E++  V KE SF I+ ++ S+V WN S+N           
Sbjct: 247 LIKEEKVDAFNIPQYTPSPAEVKYIVEKEGSFTINRLETSRVHWNASNNE---------- 306

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
             K  GYNV++ MRAVAEP+L+SHF +E+MD +F +Y EI++D M+KE T+F NV +SLT
Sbjct: 307 --KNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIVSDCMSKENTEFINVIISLT 355

Query: 301 K 302
           K
Sbjct: 367 K 355

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BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-94
Identity = 169/301 (56.15%), Postives = 224/301 (74.42%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +  S+++K VE  R+K   K  E+    NDLPGNDFNT+F+SL  F ++LR+ IG   GP
Sbjct: 67  LVVSQIVKIVEKERKKHGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGP 126

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           C F+GVP SFY RLFP KS+HFV+SSYSL WLS+VP G+E NK NIYM   SP SV+KAY
Sbjct: 127 CFFSGVPGSFYTRLFPSKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAY 186

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           Y Q++ DFS FLK R++EL+ GG+M+LTLLGR S++P SKEC YIWELL +ALN+LV +G
Sbjct: 187 YKQYEIDFSNFLKYRSEELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNELVKEG 246

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           +I+EEK+++F+IP+Y PSP E++  V KE SF I+ ++ S+V WN S+N           
Sbjct: 247 LIKEEKVDAFNIPQYTPSPAEVKYLVEKEGSFTINRLETSRVHWNASNN----------- 306

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
            +K  GYNV++ MRAVAEP+L+SHF +E+MD +F +Y EII+D M+KE T+F NV VSLT
Sbjct: 307 -VKNGGYNVSRCMRAVAEPLLVSHFDKELMDLVFHKYEEIISDCMSKEKTEFINVIVSLT 355

Query: 301 K 302
           K
Sbjct: 367 K 355

[Close]

BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot
Match: Q9SPV4 (Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 5.3e-90
Identity = 164/302 (54.30%), Postives = 220/302 (72.85%), Query Frame = 0


Query: 2   AASELIKAVEIIRQKL-KKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDV-G 61
           A +ELIK VE +R+K+ ++   EYQ+ LNDLPGNDFN IF+SLP         I  DV G
Sbjct: 69  AVTELIKTVEELRKKMGRENSPEYQIFLNDLPGNDFNAIFRSLP---------IENDVDG 128

Query: 62  PCLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKA 121
            C   GVP SFYGRLFP+ ++HF+HSSYSL WLS+VP G+E NK NIYM    P+SV+ A
Sbjct: 129 VCFINGVPGSFYGRLFPRNTLHFIHSSYSLMWLSQVPIGIESNKGNIYMANTCPQSVLNA 188

Query: 122 YYNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQ 181
           YY QFQ+D +LFL+CRAQE+V GGRM+LT+LGRRS++ AS EC  IW+LL +ALN +V +
Sbjct: 189 YYKQFQEDHALFLRCRAQEVVPGGRMVLTILGRRSEDRASTECCLIWQLLAMALNQMVSE 248

Query: 182 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVD 241
           G+IEEEK++ F+IP+Y PSP E+  E+ KE SF+ID I+ S++ W     +   K     
Sbjct: 249 GLIEEEKMDKFNIPQYTPSPTEVEAEILKEGSFLIDHIEASEIYW-----SSCTKDGDGG 308

Query: 242 VSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSL 301
            S++  GYNVA+ MRAVAEP+L+ HFGE +++++F RY+ +I +RM+KE T+F NV VSL
Sbjct: 309 GSVEEEGYNVARCMRAVAEPLLLDHFGEAIIEDVFHRYKLLIIERMSKEKTKFINVIVSL 356

[Close]

BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot
Match: A0A061FDP1 (Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 GN=TCM_034089 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.0e-74
Identity = 142/306 (46.41%), Postives = 205/306 (66.99%), Query Frame = 0


Query: 4   SELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGPCLF 63
           SE++  V+    +L     E+++ LNDL  NDFN+IF SLP F   L+ E G   G C  
Sbjct: 70  SEIMDMVQATSHRLGHPVPEFRLYLNDLYSNDFNSIFMSLPAFYHRLKEEKGIGCGSCFI 129

Query: 64  TGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEE------NKRNIYMTGNSPRSVV 123
           +GV  SFYGRLFP KS+H+VHSS SLHWLS+VP GLE       NK  +Y++ +SP SV+
Sbjct: 130 SGVAGSFYGRLFPSKSLHYVHSSSSLHWLSQVPPGLESNAVTPLNKGKVYISKSSPHSVL 189

Query: 124 KAYYNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLV 183
            AY  QFQ DF +F++ R+QELV GGRM+L+L GRRS +P ++E  Y WELL  A+  LV
Sbjct: 190 HAYSLQFQNDFPMFIESRSQELVSGGRMVLSLFGRRSTDPTTEESCYQWELLAQAIMSLV 249

Query: 184 DQGIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKS 243
            +G+IEEEK++SF+ P Y P   EI++E+ KE SF+ID ++  ++DW+    ++++ A+ 
Sbjct: 250 REGLIEEEKVDSFNTPFYAPCAEEIKVEIQKEGSFIIDRLEGFEIDWDGGAVSDVHTAQG 309

Query: 244 VDVSLKGSGYNVAKYMRAVAEPILISHF--GEEVMDELFIRYREIIADRMAKETTQFFNV 302
             +     G   AK +RAV E +L SHF  G+++MD+LF RY EI+ + ++K  T++FN+
Sbjct: 310 KLL----IGQRAAKALRAVVESMLESHFGIGQDIMDDLFSRYAEIVGNHLSKTRTKYFNL 369

[Close]

BLAST of Cucsat.G2334 vs. NCBI nr
Match: KAE8647593.1 (hypothetical protein Csa_004349 [Cucumis sativus])

HSP 1 Score: 606 bits (1563), Expect = 1.39e-218
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP
Sbjct: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY
Sbjct: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV
Sbjct: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300

Query: 301 KPR 303
           KPR
Sbjct: 301 KPR 303

[Close]

BLAST of Cucsat.G2334 vs. NCBI nr
Match: XP_011658495.2 (uncharacterized protein LOC101206474 [Cucumis sativus])

HSP 1 Score: 606 bits (1563), Expect = 2.20e-212
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP
Sbjct: 405 MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 464

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY
Sbjct: 465 CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 524

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 525 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 584

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV
Sbjct: 585 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 644

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 645 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 704

Query: 301 KPR 303
           KPR
Sbjct: 705 KPR 707

[Close]

BLAST of Cucsat.G2334 vs. NCBI nr
Match: KAA0036205.1 (salicylate carboxymethyltransferase [Cucumis melo var. makuwa] >TYK12599.1 salicylate carboxymethyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 577 bits (1486), Expect = 2.06e-206
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MA SELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFL+NLRREIGGDVGP
Sbjct: 37  MAVSELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLKNLRREIGGDVGP 96

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMT NSPRSV KAY
Sbjct: 97  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTDNSPRSVAKAY 156

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQ+D SLFLKCRAQELVDGG MILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 157 YNQFQQDLSLFLKCRAQELVDGGCMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 216

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSP EIRIEVAKE SFVIDSI+VS+VDW VS+NNE+NKAKSVD 
Sbjct: 217 IIEEEKLESFHIPKYMPSPTEIRIEVAKEGSFVIDSIRVSEVDWKVSNNNEVNKAKSVDE 276

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEE+MDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 277 SLKGSGYNVAKYMRAVAEPILISHFGEEIMDELFIRYREIIADRMAKETTQFFNVTVSLT 336

Query: 301 KPR 303
           KP+
Sbjct: 337 KPK 339

[Close]

BLAST of Cucsat.G2334 vs. NCBI nr
Match: XP_008440756.2 (PREDICTED: uncharacterized protein LOC103485074 [Cucumis melo])

HSP 1 Score: 577 bits (1486), Expect = 2.10e-200
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MA SELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFL+NLRREIGGDVGP
Sbjct: 436 MAVSELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLKNLRREIGGDVGP 495

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMT NSPRSV KAY
Sbjct: 496 CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTDNSPRSVAKAY 555

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQ+D SLFLKCRAQELVDGG MILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 556 YNQFQQDLSLFLKCRAQELVDGGCMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 615

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSP EIRIEVAKE SFVIDSI+VS+VDW VS+NNE+NKAKSVD 
Sbjct: 616 IIEEEKLESFHIPKYMPSPTEIRIEVAKEGSFVIDSIRVSEVDWKVSNNNEVNKAKSVDE 675

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEE+MDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 676 SLKGSGYNVAKYMRAVAEPILISHFGEEIMDELFIRYREIIADRMAKETTQFFNVTVSLT 735

Query: 301 KPR 303
           KP+
Sbjct: 736 KPK 738

[Close]

BLAST of Cucsat.G2334 vs. NCBI nr
Match: XP_038877344.1 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3-like [Benincasa hispida])

HSP 1 Score: 506 bits (1304), Expect = 3.09e-178
Identity = 252/303 (83.17%), Postives = 272/303 (89.77%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           M  S++IKAVE +R KL KKPIE+QVLLNDLPGNDFNTIFKSLP FL+NL+ EIGG VGP
Sbjct: 67  MVVSQVIKAVENVRGKLNKKPIEHQVLLNDLPGNDFNTIFKSLPKFLENLKLEIGGYVGP 126

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLF GVP SFYG LFP KSVHF+HSSYSLHW+  VPEGLEENKRNIYM   SP+SV+KAY
Sbjct: 127 CLFNGVPGSFYGSLFPTKSVHFIHSSYSLHWMMDVPEGLEENKRNIYMADASPKSVLKAY 186

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQKDFSLFLKCRA+ELVDGG MILTLLGR SQNPASKECSYIWELL LALNDLV QG
Sbjct: 187 YNQFQKDFSLFLKCRAEELVDGGCMILTLLGRSSQNPASKECSYIWELLALALNDLVAQG 246

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           +I++EKLESFHI KYMPSP EI+IEVAKE SFVIDSIKVSQVDWNVS+NN+ NK  S DV
Sbjct: 247 MIKKEKLESFHITKYMPSPTEIKIEVAKEGSFVIDSIKVSQVDWNVSNNNQFNKGIS-DV 306

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           S KGSGYNVAKYMRAV EPILISHFGEEVMDELFIRYREIIA+RMA+E TQFFNVTVSLT
Sbjct: 307 SSKGSGYNVAKYMRAVIEPILISHFGEEVMDELFIRYREIIAERMAEERTQFFNVTVSLT 366

Query: 301 KPR 303
           KP+
Sbjct: 367 KPK 368

[Close]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL
Match: A0A0A0KH27 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504400 PE=4 SV=1)

HSP 1 Score: 606 bits (1563), Expect = 4.81e-219
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP
Sbjct: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY
Sbjct: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV
Sbjct: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300

Query: 301 KPR 303
           KPR
Sbjct: 301 KPR 303

[Close]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL
Match: A0A5D3CQJ4 (Salicylate carboxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001830 PE=4 SV=1)

HSP 1 Score: 577 bits (1486), Expect = 9.96e-207
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MA SELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFL+NLRREIGGDVGP
Sbjct: 37  MAVSELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLKNLRREIGGDVGP 96

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMT NSPRSV KAY
Sbjct: 97  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTDNSPRSVAKAY 156

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQ+D SLFLKCRAQELVDGG MILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 157 YNQFQQDLSLFLKCRAQELVDGGCMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 216

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSP EIRIEVAKE SFVIDSI+VS+VDW VS+NNE+NKAKSVD 
Sbjct: 217 IIEEEKLESFHIPKYMPSPTEIRIEVAKEGSFVIDSIRVSEVDWKVSNNNEVNKAKSVDE 276

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEE+MDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 277 SLKGSGYNVAKYMRAVAEPILISHFGEEIMDELFIRYREIIADRMAKETTQFFNVTVSLT 336

Query: 301 KPR 303
           KP+
Sbjct: 337 KPK 339

[Close]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL
Match: A0A1S3B1F0 (uncharacterized protein LOC103485074 OS=Cucumis melo OX=3656 GN=LOC103485074 PE=4 SV=1)

HSP 1 Score: 577 bits (1486), Expect = 1.02e-200
Identity = 286/303 (94.39%), Postives = 294/303 (97.03%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MA SELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFL+NLRREIGGDVGP
Sbjct: 436 MAVSELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLKNLRREIGGDVGP 495

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMT NSPRSV KAY
Sbjct: 496 CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTDNSPRSVAKAY 555

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           YNQFQ+D SLFLKCRAQELVDGG MILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG
Sbjct: 556 YNQFQQDLSLFLKCRAQELVDGGCMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 615

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           IIEEEKLESFHIPKYMPSP EIRIEVAKE SFVIDSI+VS+VDW VS+NNE+NKAKSVD 
Sbjct: 616 IIEEEKLESFHIPKYMPSPTEIRIEVAKEGSFVIDSIRVSEVDWKVSNNNEVNKAKSVDE 675

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
           SLKGSGYNVAKYMRAVAEPILISHFGEE+MDELFIRYREIIADRMAKETTQFFNVTVSLT
Sbjct: 676 SLKGSGYNVAKYMRAVAEPILISHFGEEIMDELFIRYREIIADRMAKETTQFFNVTVSLT 735

Query: 301 KPR 303
           KP+
Sbjct: 736 KPK 738

[Close]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL
Match: A0A6J1BSI5 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005311 PE=4 SV=1)

HSP 1 Score: 471 bits (1213), Expect = 9.45e-165
Identity = 238/302 (78.81%), Postives = 259/302 (85.76%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGD-VG 60
           MA S+LIKAVE  R+KL K PIEYQV LNDLPGNDFNTIF+SLP FL++ + EIGGD +G
Sbjct: 67  MAVSQLIKAVENARRKLCKDPIEYQVFLNDLPGNDFNTIFRSLPGFLESFKMEIGGDDLG 126

Query: 61  PCLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKA 120
           PCLFTGVP SFYGRLFP +SVHFVHSSYSLHWLS VP GLEENKRNIYM   SP+SVV A
Sbjct: 127 PCLFTGVPGSFYGRLFPSRSVHFVHSSYSLHWLSDVPRGLEENKRNIYMAETSPKSVVSA 186

Query: 121 YYNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQ 180
           YYNQFQKDFSLFLKCRA+ELV+GG M+LTLLGRRSQNPASKECSYIWELL LAL+D+V Q
Sbjct: 187 YYNQFQKDFSLFLKCRAEELVEGGCMVLTLLGRRSQNPASKECSYIWELLALALDDMVSQ 246

Query: 181 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVD 240
           G IEEEKLESFHIPKYMPSP EI+IEVAKE SF ID IKVS+VDWNV +NN  +   S  
Sbjct: 247 GKIEEEKLESFHIPKYMPSPKEIKIEVAKEGSFGIDCIKVSEVDWNVYNNNNHSNDGS-- 306

Query: 241 VSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSL 300
              KGSGYNVAK MRAVAEPILISHFGE ++ ELFI+YREIIADRMAKE TQF NVTVSL
Sbjct: 307 ---KGSGYNVAKCMRAVAEPILISHFGEHIIHELFIKYREIIADRMAKEKTQFVNVTVSL 363

[Close]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL
Match: A0A6J1KYB3 (salicylate carboxymethyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111497465 PE=4 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 4.34e-158
Identity = 218/301 (72.43%), Postives = 253/301 (84.05%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           MA S+LIK VE IR  L   PIEYQV LNDLPGNDFN IFK+LPNF +N ++E+G D+GP
Sbjct: 67  MAVSQLIKTVENIRTNLCNNPIEYQVFLNDLPGNDFNAIFKALPNFHENFKKEMGADLGP 126

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           CLFTGVP SFYGRLFP +SVHF+HSSYSLHWLSKVPEGLEENKRNIYM   SP SV+KAY
Sbjct: 127 CLFTGVPGSFYGRLFPSRSVHFIHSSYSLHWLSKVPEGLEENKRNIYMAETSPASVLKAY 186

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECSYIWELLGLALNDLVDQG 180
           Y+QFQKDFSLFL+CRA+ELVDGG M+LTLLGR S++P SKEC YIWELL +ALND+V QG
Sbjct: 187 YDQFQKDFSLFLRCRAEELVDGGYMVLTLLGRSSEDPTSKECCYIWELLAMALNDIVSQG 246

Query: 181 IIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVDV 240
           +IEEEKLESF++PKYMPSP E+ IE+ KE SFV++ I+VS+VDW+V +NN+ +       
Sbjct: 247 MIEEEKLESFNLPKYMPSPTEMSIEIGKEGSFVVNRIQVSKVDWHVYNNNDESNG----- 306

Query: 241 SLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSLT 300
            L+  GYNVAKYMRAVAEPIL+SHFGE +MDELFIRY EII DRMAKE  +F NVTVSLT
Sbjct: 307 -LRAGGYNVAKYMRAVAEPILVSHFGEAIMDELFIRYGEIIVDRMAKEKPEFVNVTVSLT 361

[Close]

BLAST of Cucsat.G2334 vs. TAIR 10
Match: AT5G04370.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.5e-66
Identity = 129/304 (42.43%), Postives = 188/304 (61.84%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +A SE+I  + +  Q+  + P E    LNDLP NDFNT FK    F+Q            
Sbjct: 100 LAMSEIINTINVFCQQRNQNPPEIDCCLNDLPSNDFNTTFK----FIQFFNGMNITSKES 159

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
               GVP SFY RLFP++S+HFVHSSY LHWLSKVPEGLE+NK ++Y+T +SP S  KAY
Sbjct: 160 YFVYGVPGSFYSRLFPRRSLHFVHSSYGLHWLSKVPEGLEKNKMSVYITNSSPLSTYKAY 219

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRS-QNPASKECSYIWELLGLALNDLVDQ 180
            NQFQ+DF+ FLK R++E+V  GRM+LT +GR +  NP  ++C + W LL  +L DLV +
Sbjct: 220 LNQFQRDFATFLKLRSEEMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAE 279

Query: 181 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVD 240
           G++   K++SF++P Y P+  EI+  V KE SF I  ++    D    + +E  ++K   
Sbjct: 280 GLVSASKVDSFYLPFYDPNEKEIKEMVQKEGSFEIRDLETHGYDLGHCNQDESKRSK--- 339

Query: 241 VSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSL 300
                SG N A Y+RAV+EP+L +HFG+ +++ LF ++   ++  ++       ++ VSL
Sbjct: 340 -----SGQNEANYIRAVSEPLLAAHFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSL 391

Query: 301 TKPR 304
           TK +
Sbjct: 400 TKKK 391

[Close]

BLAST of Cucsat.G2334 vs. TAIR 10
Match: AT5G04370.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.5e-66
Identity = 129/304 (42.43%), Postives = 188/304 (61.84%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +A SE+I  + +  Q+  + P E    LNDLP NDFNT FK    F+Q            
Sbjct: 119 LAMSEIINTINVFCQQRNQNPPEIDCCLNDLPSNDFNTTFK----FIQFFNGMNITSKES 178

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
               GVP SFY RLFP++S+HFVHSSY LHWLSKVPEGLE+NK ++Y+T +SP S  KAY
Sbjct: 179 YFVYGVPGSFYSRLFPRRSLHFVHSSYGLHWLSKVPEGLEKNKMSVYITNSSPLSTYKAY 238

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRS-QNPASKECSYIWELLGLALNDLVDQ 180
            NQFQ+DF+ FLK R++E+V  GRM+LT +GR +  NP  ++C + W LL  +L DLV +
Sbjct: 239 LNQFQRDFATFLKLRSEEMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAE 298

Query: 181 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVD 240
           G++   K++SF++P Y P+  EI+  V KE SF I  ++    D    + +E  ++K   
Sbjct: 299 GLVSASKVDSFYLPFYDPNEKEIKEMVQKEGSFEIRDLETHGYDLGHCNQDESKRSK--- 358

Query: 241 VSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSL 300
                SG N A Y+RAV+EP+L +HFG+ +++ LF ++   ++  ++       ++ VSL
Sbjct: 359 -----SGQNEANYIRAVSEPLLAAHFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSL 410

Query: 301 TKPR 304
           TK +
Sbjct: 419 TKKK 410

[Close]

BLAST of Cucsat.G2334 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.5e-63
Identity = 128/305 (41.97%), Postives = 186/305 (60.98%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +A  E+I  + ++ Q + K   E    LNDLP NDFNT FK +P F + L   +  +   
Sbjct: 90  LAIFEIINTINVLCQHVNKNSPEIDCCLNDLPENDFNTTFKFVPFFNKEL---MITNKSS 149

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           C   G P SFY RLF + S+H +HSSY+LHWLSKVPE LE NK N+Y+T +SP+S  KAY
Sbjct: 150 CFVYGAPGSFYSRLFSRNSLHLIHSSYALHWLSKVPEKLENNKGNLYITSSSPQSAYKAY 209

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQN-PASKECSYIWELLGLALNDLVDQ 180
            NQFQKDF++FL+ R++E+V  GRM+LT +GR + N P  ++C + W LL  +L DLV +
Sbjct: 210 LNQFQKDFTMFLRLRSEEIVSNGRMVLTFIGRNTLNDPLYRDCCHFWTLLSNSLRDLVFE 269

Query: 181 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVD---WNVSDNNEMNKAK 240
           G++ E KL++F++P Y P+  E++  + KE SF I+ ++    D   +   D+ E     
Sbjct: 270 GLVSESKLDAFNMPFYDPNVQELKEVIQKEGSFEINELESHGFDLGHYYEEDDFE----- 329

Query: 241 SVDVSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVT 300
                   +G N A  +RAV+EP+LI+HFGEE++D LF +Y   +            ++ 
Sbjct: 330 --------AGRNEANGIRAVSEPMLIAHFGEEIIDTLFDKYAYHVTQHANCRNKTTVSLV 378

Query: 301 VSLTK 302
           VSLTK
Sbjct: 390 VSLTK 378

[Close]

BLAST of Cucsat.G2334 vs. TAIR 10
Match: AT5G66430.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 5.7e-63
Identity = 119/302 (39.40%), Postives = 189/302 (62.58%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNFLQNLRREIGGDVGP 60
           +  +E++  + ++ Q+  +KP E    LNDLP NDFNT FK +P F + ++ +       
Sbjct: 60  LTMAEIVNTINVLCQQCNQKPPEIDCCLNDLPDNDFNTTFKFVPFFNKRVKSK-----RL 119

Query: 61  CLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVPEGLEENKRNIYMTGNSPRSVVKAY 120
           C  +GVP SFY RLFP+KS+HFVHSSYSLHWLSKVP+GLE+N  ++Y+T +SP +  KAY
Sbjct: 120 CFVSGVPGSFYSRLFPRKSLHFVHSSYSLHWLSKVPKGLEKNSSSVYITTSSPPNAYKAY 179

Query: 121 YNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRS-QNPASKECSYIWELLGLALNDLVDQ 180
            NQFQ DF  FL+ R++E+V  GRM+LT +GR++  +P  ++C + W LL  +L DLV +
Sbjct: 180 LNQFQSDFKSFLEMRSEEMVSNGRMVLTFIGRKTLDDPLHRDCCHFWTLLSTSLRDLVYE 239

Query: 181 GIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVDWNVSDNNEMNKAKSVD 240
           G++   K++SF+IP Y PS  E+   +  E SF I+ +++   +  +S+++E     S  
Sbjct: 240 GLVSASKVDSFNIPFYDPSKEEVMEMIRNEGSFEINDLEIHGFELGLSNHDEDYMLHS-- 299

Query: 241 VSLKGSGYNVAKYMRAVAEPILISHFGEEVMDELFIRYREIIADRMAKETTQFFNVTVSL 300
             +  +G   A  +RAV+E +L++ FG ++MD LF ++   ++   +        + VSL
Sbjct: 300 -QISKAGQREANCIRAVSESMLVADFGVDIMDTLFKKFAYHVSQHASCTNKTTVTLVVSL 353

Query: 301 TK 302
            +
Sbjct: 360 IR 353

[Close]

BLAST of Cucsat.G2334 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 236.5 bits (602), Expect = 2.8e-62
Identity = 135/324 (41.67%), Postives = 193/324 (59.57%), Query Frame = 0


Query: 1   MAASELIKAVEIIRQKLKKKPIEYQVLLNDLPGNDFNTIFKSLPNF---LQNLRREIG-- 60
           ++ S ++  +  +   L +   E +V LNDLP NDFN I  SLP F   + N +  +G  
Sbjct: 68  LSISNIVDTIHNLCPDLDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNNKEGLGFG 127

Query: 61  -GDVGPCLFTGVPASFYGRLFPKKSVHFVHSSYSLHWLSKVP-----------EGLEENK 120
            G    C  + VP SFYGRLFP++S+HFVHSS SLHWLS+VP               EN 
Sbjct: 128 RGGGESCFVSAVPGSFYGRLFPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTITADLENM 187

Query: 121 RNIYMTGNSPRSVVKAYYNQFQKDFSLFLKCRAQELVDGGRMILTLLGRRSQNPASKECS 180
             IY++  SP+S  KAY  QFQ DF +FL+ R++ELV GGRM+L+ LGRRS +P ++E  
Sbjct: 188 GKIYISKTSPKSAHKAYALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDPTTEESC 247

Query: 181 YIWELLGLALNDLVDQGIIEEEKLESFHIPKYMPSPIEIRIEVAKEASFVIDSIKVSQVD 240
           Y WELL  AL  +  +GIIEEEK+++F+ P Y  S  E+++ + KE SF ID +++S +D
Sbjct: 248 YQWELLAQALMSMAKEGIIEEEKIDAFNAPYYAASSEELKMVIEKEGSFSIDRLEISPID 307

Query: 241 WNVSDNNEMNKAKSVDVSLK------GSGYNVAKYMRAVAEPILISHFGEEVMDELFIRY 300
           W     +E    +S D+ ++       SG  V+  +RAV EP+L   FGE VMDELF RY
Sbjct: 308 WEGGSISE----ESYDLVIRSKPEALASGRRVSNTIRAVVEPMLEPTFGENVMDELFERY 367

Query: 301 REIIADRMAKETTQFFNVTVSLTK 302
            +I+ +     + ++  V +SL +
Sbjct: 368 AKIVGEYFYVSSPRYAIVILSLVR 387

The following BLAST results are available for this feature:

BLAST of Cucsat.G2334 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84UB4	9.3e-95	56.48	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2...	[more]
Q84UB5	1.2e-94	55.81	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1...	[more]
A4ZDG8	1.2e-94	56.15	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3...	[more]
Q9SPV4	5.3e-90	54.30	Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=...	[more]
A0A061FDP1	7.0e-74	46.41	Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 G...	[more]

BLAST of Cucsat.G2334 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647593.1	1.39e-218	100.00	hypothetical protein Csa_004349 [Cucumis sativus]	[more]
XP_011658495.2	2.20e-212	100.00	uncharacterized protein LOC101206474 [Cucumis sativus]	[more]
KAA0036205.1	2.06e-206	94.39	salicylate carboxymethyltransferase [Cucumis melo var. makuwa] >TYK12599.1 salic...	[more]
XP_008440756.2	2.10e-200	94.39	PREDICTED: uncharacterized protein LOC103485074 [Cucumis melo]	[more]
XP_038877344.1	3.09e-178	83.17	S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3...	[more]

BLAST of Cucsat.G2334 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KH27	4.81e-219	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504400 PE=4 SV=1	[more]
A0A5D3CQJ4	9.96e-207	94.39	Salicylate carboxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3B1F0	1.02e-200	94.39	uncharacterized protein LOC103485074 OS=Cucumis melo OX=3656 GN=LOC103485074 PE=...	[more]
A0A6J1BSI5	9.45e-165	78.81	salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1KYB3	4.34e-158	72.43	salicylate carboxymethyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]

BLAST of Cucsat.G2334 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04370.1	2.5e-66	42.43	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G04370.2	2.5e-66	42.43	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G11480.1	2.5e-63	41.97	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G66430.1	5.7e-63	39.40	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G19640.1	2.8e-62	41.67	jasmonic acid carboxyl methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005299	SAM dependent carboxyl methyltransferase	PFAM	PF03492	Methyltransf_7	coord: 3..301 e-value: 3.7E-108 score: 361.7
IPR005299	SAM dependent carboxyl methyltransferase	PANTHER	PTHR31009	S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN	coord: 2..301
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 2..289 e-value: 1.5E-111 score: 374.6
None	No IPR available	PANTHER	PTHR31009:SF98	SAM DEPENDENT CARBOXYL METHYLTRANSFERASE-RELATED	coord: 2..301
IPR042086	Methyltransferase, alpha-helical capping domain	GENE3D	1.10.1200.270		coord: 94..288 e-value: 1.5E-111 score: 374.6
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 2..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G2334.T1	Cucsat.G2334.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity