Cucsat.G225 (gene) Cucumber (B10) v3

BLAST of Cucsat.G225 vs. NCBI nr
Match: XP_004149308.2 (uncharacterized protein LOC101210213 [Cucumis sativus] >KGN56210.1 hypothetical protein Csa_010126 [Cucumis sativus])

HSP 1 Score: 657 bits (1695), Expect = 2.13e-237
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60
           MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY
Sbjct: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60

Query: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120
           LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA
Sbjct: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120

Query: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180
           NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP
Sbjct: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180

Query: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240
           RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS
Sbjct: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240

Query: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE 300
           MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE
Sbjct: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE 300

Query: 301 ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
           ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF
Sbjct: 301 ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346

BLAST of Cucsat.G225 vs. NCBI nr
Match: XP_008452289.1 (PREDICTED: uncharacterized protein LOC103493359 [Cucumis melo] >KAA0046874.1 uncharacterized protein E6C27_scaffold230G00290 [Cucumis melo var. makuwa] >TYK03272.1 uncharacterized protein E5676_scaffold298G00990 [Cucumis melo var. makuwa])

HSP 1 Score: 600 bits (1547), Expect = 8.81e-215
Identity = 317/350 (90.57%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60
           MPILSSNYHWILSLKIL ISTG+LSMALFLKLSVP++ADFV SEIPSIWTSFLSWLTPPY
Sbjct: 1   MPILSSNYHWILSLKILFISTGVLSMALFLKLSVPVVADFVLSEIPSIWTSFLSWLTPPY 60

Query: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120
           LYLLINCIIISIVASSKLQS LENDPIPETPAPPPV+KI SDYAVCG SDILNGYSSYNA
Sbjct: 61  LYLLINCIIISIVASSKLQSKLENDPIPETPAPPPVSKIPSDYAVCGGSDILNGYSSYNA 120

Query: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180
           NQNVVTKVSDLEIDDSNE+YG+IEESRV EMEKKGENDS+IAMKG DESSVLS ITNTLP
Sbjct: 121 NQNVVTKVSDLEIDDSNELYGQIEESRVFEMEKKGENDSVIAMKGSDESSVLSPITNTLP 180

Query: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240
           R DSIGVLFSNK+EKPPVSSRFGQRKFVK+SPEGKPLGVSKPKRQDTLENTWRKITEGRS
Sbjct: 181 RNDSIGVLFSNKKEKPPVSSRFGQRKFVKASPEGKPLGVSKPKRQDTLENTWRKITEGRS 240

Query: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP----GGSGR 300
           MPLTRHLRKSDTWESHGR++PTMVVE    PPSKVMKKSETFKEGRGGGSP    GGSGR
Sbjct: 241 MPLTRHLRKSDTWESHGRRSPTMVVEPATPPPSKVMKKSETFKEGRGGGSPSPSPGGSGR 300

Query: 301 MKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
           +KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEM+GRGGAQSF
Sbjct: 301 LKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMMGRGGAQSF 350

BLAST of Cucsat.G225 vs. NCBI nr
Match: XP_038905377.1 (uncharacterized protein LOC120091427 [Benincasa hispida])

HSP 1 Score: 522 bits (1344), Expect = 8.31e-184
Identity = 287/356 (80.62%), Postives = 312/356 (87.64%), Query Frame = 0

Query: 1   MPILS--SNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTP 60
           MPILS  SNY WILSLKI+LISTG+L MA+FLKLSVP++ADFV S IPSIWT FLSWLTP
Sbjct: 1   MPILSFNSNYQWILSLKIVLISTGVLFMAVFLKLSVPVVADFVLSGIPSIWTCFLSWLTP 60

Query: 61  PYLYLLINCIIISIVASSKLQSNLENDP----IPETPAPPPVTKISSDYAVCGSSDILNG 120
           PYLYLLINCIIISIVASSKLQS LE+D     IPETPAPPPV KISSDYAVCG +DILNG
Sbjct: 61  PYLYLLINCIIISIVASSKLQSKLEDDASVSLIPETPAPPPVAKISSDYAVCGGTDILNG 120

Query: 121 YSSYNANQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSS 180
           YSSYNAN NVVTK+SDL+ID+SN+VYG++ ESRVSEMEKK ENDS+I MK GDES V S 
Sbjct: 121 YSSYNANPNVVTKISDLQIDESNDVYGQMVESRVSEMEKKVENDSLIVMKAGDESDVSSP 180

Query: 181 ITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRK 240
           IT  LPRKDSIG+LFSN++EKPPVSSR GQRKFVK+SPEGKPLGVSKPKRQDTLE+TWR 
Sbjct: 181 ITTALPRKDSIGLLFSNEKEKPPVSSRIGQRKFVKASPEGKPLGVSKPKRQDTLESTWRT 240

Query: 241 ITEGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP--- 300
           ITEGRSMPLTRHLRKSDTWESHGR+   + V DPATPPSKVMKKSETF E   GGSP   
Sbjct: 241 ITEGRSMPLTRHLRKSDTWESHGRRGAAIAV-DPATPPSKVMKKSETFNERSNGGSPSPS 300

Query: 301 -GGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
            GGSGR++RE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEM+GRG A SF
Sbjct: 301 PGGSGRLRREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMMGRG-AHSF 354

BLAST of Cucsat.G225 vs. NCBI nr
Match: XP_022985244.1 (uncharacterized protein LOC111483295 [Cucurbita maxima])

HSP 1 Score: 471 bits (1212), Expect = 8.39e-164
Identity = 267/352 (75.85%), Postives = 294/352 (83.52%), Query Frame = 0

Query: 1   MPILSSN--YHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTP 60
           M ILSSN  Y WILSLKILL+STG+LSMA+FLKLSVP+++DF+ SEIPSIWT FLSWLTP
Sbjct: 1   MSILSSNSSYQWILSLKILLVSTGVLSMAVFLKLSVPVVSDFIVSEIPSIWTCFLSWLTP 60

Query: 61  PYLYLLINCIIISIVASSKLQSNLEND----PIPETPAPPPVTKISSDYAVCGSSDILNG 120
           PYLYLLINCIIISIVASSK Q  LE+D    PIP T AP PV KISS YAVCG S+I NG
Sbjct: 61  PYLYLLINCIIISIVASSKFQPKLEDDASVPPIPHTIAPFPVAKISSAYAVCGGSEIFNG 120

Query: 121 YSSYNANQNVVTKVSDLEIDDSNEVYGRIE-ESRVSEMEKKGENDSMIAMKGGDESSVLS 180
           YS YNA  NV T VSDL+I++SN VYGR+  ESRVSEMEKKGENDS+I  K  DES V S
Sbjct: 121 YS-YNAIPNVATNVSDLQIEESNGVYGRMNVESRVSEMEKKGENDSLI--KASDESGVSS 180

Query: 181 SITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWR 240
            +TN L RKDSIG++FSN+ E+PPVSSR GQRKFVK+SPEGKPLGVSKPKRQ+T E+TWR
Sbjct: 181 PMTNALQRKDSIGLIFSNENERPPVSSRIGQRKFVKASPEGKPLGVSKPKRQETFESTWR 240

Query: 241 KITEGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP-- 300
            ITE RSMPLTRHLRKSDTWESHGR+   +V  DPATPPSKVMKKSETF +   GGSP  
Sbjct: 241 TITERRSMPLTRHLRKSDTWESHGRRGAALV--DPATPPSKVMKKSETFNDRSSGGSPSP 300

Query: 301 --GGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 341
             GGSGR+KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVG G
Sbjct: 301 SPGGSGRVKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGHG 347

BLAST of Cucsat.G225 vs. NCBI nr
Match: KAG6577392.1 (hypothetical protein SDJN03_24966, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471 bits (1211), Expect = 1.19e-163
Identity = 268/352 (76.14%), Postives = 294/352 (83.52%), Query Frame = 0

Query: 1   MPILSSN--YHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTP 60
           M ILSSN  Y WILSLKILL+STG+LSMA+FLKLSVP+++DF+ SEIPSIWT FLSWLTP
Sbjct: 1   MSILSSNSSYQWILSLKILLVSTGVLSMAVFLKLSVPVVSDFIVSEIPSIWTCFLSWLTP 60

Query: 61  PYLYLLINCIIISIVASSKLQSNLEND----PIPETPAPPPVTKISSDYAVCGSSDILNG 120
           PYLYLLINCIIISIVASSK Q  LE+D    PIP+T AP PV KISS YAVCG S+I NG
Sbjct: 61  PYLYLLINCIIISIVASSKFQPKLEDDASVPPIPQTIAPFPVAKISSAYAVCGGSEIFNG 120

Query: 121 YSSYNANQNVVTKVSDLEIDDSNEVYGRIE-ESRVSEMEKKGENDSMIAMKGGDESSVLS 180
           YS YNA  NV T VSDL+I++SN VYGR++ ESRVSEMEKKGENDS+I  K  DES VLS
Sbjct: 121 YS-YNAIPNVATNVSDLQIEESNGVYGRMKVESRVSEMEKKGENDSLI--KASDESRVLS 180

Query: 181 SITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWR 240
            ITN L RKDSIG+ FSN+ E+PPVSSR GQRK VK+SPEGKPLGVSKPKRQ+TLE+TWR
Sbjct: 181 PITNALQRKDSIGLTFSNENERPPVSSRIGQRKLVKASPEGKPLGVSKPKRQETLESTWR 240

Query: 241 KITEGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP-- 300
            ITE RSMPLTRHLRKSDTWESHGR+   +V  DPATPPSKVMKKSETF E   GGSP  
Sbjct: 241 TITERRSMPLTRHLRKSDTWESHGRRGAALV--DPATPPSKVMKKSETFNERSSGGSPSP 300

Query: 301 --GGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 341
             G SGR+KRE S +QDELNRRVEAFIKKFNDEMRLQRQES KQYQEMVG G
Sbjct: 301 SPGSSGRVKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESFKQYQEMVGHG 347

BLAST of Cucsat.G225 vs. ExPASy TrEMBL
Match: A0A0A0L2K5 (Fiber expressed protein OS=Cucumis sativus OX=3659 GN=Csa_3G099710 PE=4 SV=1)

HSP 1 Score: 657 bits (1695), Expect = 1.03e-237
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60
           MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY
Sbjct: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60

Query: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120
           LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA
Sbjct: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120

Query: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180
           NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP
Sbjct: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180

Query: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240
           RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS
Sbjct: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240

Query: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE 300
           MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE
Sbjct: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKRE 300

Query: 301 ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
           ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF
Sbjct: 301 ASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346

BLAST of Cucsat.G225 vs. ExPASy TrEMBL
Match: A0A5D3BWR5 (DUF4408 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G00990 PE=4 SV=1)

HSP 1 Score: 600 bits (1547), Expect = 4.26e-215
Identity = 317/350 (90.57%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60
           MPILSSNYHWILSLKIL ISTG+LSMALFLKLSVP++ADFV SEIPSIWTSFLSWLTPPY
Sbjct: 1   MPILSSNYHWILSLKILFISTGVLSMALFLKLSVPVVADFVLSEIPSIWTSFLSWLTPPY 60

Query: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120
           LYLLINCIIISIVASSKLQS LENDPIPETPAPPPV+KI SDYAVCG SDILNGYSSYNA
Sbjct: 61  LYLLINCIIISIVASSKLQSKLENDPIPETPAPPPVSKIPSDYAVCGGSDILNGYSSYNA 120

Query: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180
           NQNVVTKVSDLEIDDSNE+YG+IEESRV EMEKKGENDS+IAMKG DESSVLS ITNTLP
Sbjct: 121 NQNVVTKVSDLEIDDSNELYGQIEESRVFEMEKKGENDSVIAMKGSDESSVLSPITNTLP 180

Query: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240
           R DSIGVLFSNK+EKPPVSSRFGQRKFVK+SPEGKPLGVSKPKRQDTLENTWRKITEGRS
Sbjct: 181 RNDSIGVLFSNKKEKPPVSSRFGQRKFVKASPEGKPLGVSKPKRQDTLENTWRKITEGRS 240

Query: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP----GGSGR 300
           MPLTRHLRKSDTWESHGR++PTMVVE    PPSKVMKKSETFKEGRGGGSP    GGSGR
Sbjct: 241 MPLTRHLRKSDTWESHGRRSPTMVVEPATPPPSKVMKKSETFKEGRGGGSPSPSPGGSGR 300

Query: 301 MKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
           +KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEM+GRGGAQSF
Sbjct: 301 LKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMMGRGGAQSF 350

BLAST of Cucsat.G225 vs. ExPASy TrEMBL
Match: A0A1S3BTJ4 (uncharacterized protein LOC103493359 OS=Cucumis melo OX=3656 GN=LOC103493359 PE=4 SV=1)

HSP 1 Score: 600 bits (1547), Expect = 4.26e-215
Identity = 317/350 (90.57%), Postives = 331/350 (94.57%), Query Frame = 0

Query: 1   MPILSSNYHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPY 60
           MPILSSNYHWILSLKIL ISTG+LSMALFLKLSVP++ADFV SEIPSIWTSFLSWLTPPY
Sbjct: 1   MPILSSNYHWILSLKILFISTGVLSMALFLKLSVPVVADFVLSEIPSIWTSFLSWLTPPY 60

Query: 61  LYLLINCIIISIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNA 120
           LYLLINCIIISIVASSKLQS LENDPIPETPAPPPV+KI SDYAVCG SDILNGYSSYNA
Sbjct: 61  LYLLINCIIISIVASSKLQSKLENDPIPETPAPPPVSKIPSDYAVCGGSDILNGYSSYNA 120

Query: 121 NQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 180
           NQNVVTKVSDLEIDDSNE+YG+IEESRV EMEKKGENDS+IAMKG DESSVLS ITNTLP
Sbjct: 121 NQNVVTKVSDLEIDDSNELYGQIEESRVFEMEKKGENDSVIAMKGSDESSVLSPITNTLP 180

Query: 181 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWRKITEGRS 240
           R DSIGVLFSNK+EKPPVSSRFGQRKFVK+SPEGKPLGVSKPKRQDTLENTWRKITEGRS
Sbjct: 181 RNDSIGVLFSNKKEKPPVSSRFGQRKFVKASPEGKPLGVSKPKRQDTLENTWRKITEGRS 240

Query: 241 MPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP----GGSGR 300
           MPLTRHLRKSDTWESHGR++PTMVVE    PPSKVMKKSETFKEGRGGGSP    GGSGR
Sbjct: 241 MPLTRHLRKSDTWESHGRRSPTMVVEPATPPPSKVMKKSETFKEGRGGGSPSPSPGGSGR 300

Query: 301 MKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRGGAQSF 346
           +KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEM+GRGGAQSF
Sbjct: 301 LKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMMGRGGAQSF 350

BLAST of Cucsat.G225 vs. ExPASy TrEMBL
Match: A0A6J1JCS1 (uncharacterized protein LOC111483295 OS=Cucurbita maxima OX=3661 GN=LOC111483295 PE=4 SV=1)

HSP 1 Score: 471 bits (1212), Expect = 4.06e-164
Identity = 267/352 (75.85%), Postives = 294/352 (83.52%), Query Frame = 0

Query: 1   MPILSSN--YHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTP 60
           M ILSSN  Y WILSLKILL+STG+LSMA+FLKLSVP+++DF+ SEIPSIWT FLSWLTP
Sbjct: 1   MSILSSNSSYQWILSLKILLVSTGVLSMAVFLKLSVPVVSDFIVSEIPSIWTCFLSWLTP 60

Query: 61  PYLYLLINCIIISIVASSKLQSNLEND----PIPETPAPPPVTKISSDYAVCGSSDILNG 120
           PYLYLLINCIIISIVASSK Q  LE+D    PIP T AP PV KISS YAVCG S+I NG
Sbjct: 61  PYLYLLINCIIISIVASSKFQPKLEDDASVPPIPHTIAPFPVAKISSAYAVCGGSEIFNG 120

Query: 121 YSSYNANQNVVTKVSDLEIDDSNEVYGRIE-ESRVSEMEKKGENDSMIAMKGGDESSVLS 180
           YS YNA  NV T VSDL+I++SN VYGR+  ESRVSEMEKKGENDS+I  K  DES V S
Sbjct: 121 YS-YNAIPNVATNVSDLQIEESNGVYGRMNVESRVSEMEKKGENDSLI--KASDESGVSS 180

Query: 181 SITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWR 240
            +TN L RKDSIG++FSN+ E+PPVSSR GQRKFVK+SPEGKPLGVSKPKRQ+T E+TWR
Sbjct: 181 PMTNALQRKDSIGLIFSNENERPPVSSRIGQRKFVKASPEGKPLGVSKPKRQETFESTWR 240

Query: 241 KITEGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP-- 300
            ITE RSMPLTRHLRKSDTWESHGR+   +V  DPATPPSKVMKKSETF +   GGSP  
Sbjct: 241 TITERRSMPLTRHLRKSDTWESHGRRGAALV--DPATPPSKVMKKSETFNDRSSGGSPSP 300

Query: 301 --GGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 341
             GGSGR+KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVG G
Sbjct: 301 SPGGSGRVKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGHG 347

BLAST of Cucsat.G225 vs. ExPASy TrEMBL
Match: A0A6J1ETQ9 (uncharacterized protein LOC111435872 OS=Cucurbita moschata OX=3662 GN=LOC111435872 PE=4 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.91e-162
Identity = 265/352 (75.28%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 1   MPILSSN--YHWILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTP 60
           M ILSSN  Y WILSLKILL+STG+LSMA+FLKLSVP+++DF+ SEIPSIWT FLSWLTP
Sbjct: 1   MSILSSNSSYQWILSLKILLVSTGVLSMAVFLKLSVPVVSDFIVSEIPSIWTCFLSWLTP 60

Query: 61  PYLYLLINCIIISIVASSKLQSNLEND----PIPETPAPPPVTKISSDYAVCGSSDILNG 120
           PYLYLLINCIIISIVASSK Q  LE+D    PIP+T AP PV KISS YAVCG S+I NG
Sbjct: 61  PYLYLLINCIIISIVASSKFQPKLEDDASVPPIPQTIAPFPVAKISSAYAVCGGSEIFNG 120

Query: 121 YSSYNANQNVVTKVSDLEIDDSNEVYGRIE-ESRVSEMEKKGENDSMIAMKGGDESSVLS 180
           Y  YNA  NV T VSDL+I++SN VYGR++ ESRVSEMEKKGENDS+I  K  DES V S
Sbjct: 121 YC-YNAIPNVATNVSDLQIEESNGVYGRMKVESRVSEMEKKGENDSLI--KASDESGVSS 180

Query: 181 SITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEGKPLGVSKPKRQDTLENTWR 240
            +TN+L RKDSIG++FSN+ E+PPVSSR GQRK VK+SPEGKPLGVSKPKRQ+TLE+TWR
Sbjct: 181 PMTNSLQRKDSIGLMFSNENERPPVSSRIGQRKLVKASPEGKPLGVSKPKRQETLESTWR 240

Query: 241 KITEGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSP-- 300
            ITE RSMPLTRHLRKSDTWESHGR+   +V  DPATPPSKVMKKSETF +   G SP  
Sbjct: 241 TITERRSMPLTRHLRKSDTWESHGRRGAALV--DPATPPSKVMKKSETFNDRSSGRSPSP 300

Query: 301 --GGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 341
             GGSGR+KRE S +QDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVG G
Sbjct: 301 SPGGSGRVKREPSLTQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGHG 347

BLAST of Cucsat.G225 vs. TAIR 10
Match: AT1G61260.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 179.9 bits (455), Expect = 3.6e-45
Identity = 141/354 (39.83%), Postives = 203/354 (57.34%), Query Frame = 0

Query: 10  WILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPYLYLLINCII 69
           W+++ K +LIS+G+ ++AL LKLSVP+  DF  S  P +W+S LSWL PPYLY++ N II
Sbjct: 4   WMMTTKAVLISSGVATVALLLKLSVPVAVDFSVSRAPILWSSLLSWLKPPYLYVVTNGII 63

Query: 70  ISIVASSK-LQSNLENDPIPE-----------TPAPPPVTKISSDYAVCGSSDILNG--- 129
           I+IVASSK  +S+ + D   E               P V +  +   +    D+  G   
Sbjct: 64  ITIVASSKYYRSHHDRDEEDEIVVYGGGGYKIQTEEPIVNQHQASPRILEVKDLDTGAHF 123

Query: 130 ---YSSYNANQNVVTKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGD--ES 189
               ++  A +     V+ +  DD  E    I+ +  +E E + E  S+I ++  D  ES
Sbjct: 124 GFVVANLEAEELESEAVTAVVFDDEEEEKKIIDSAATAEDEIEEELKSVIMVENSDLVES 183

Query: 190 SVLSSITNTLPRKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPE-GKPLGVSKPKRQDTL 249
            V       +P    I        EKP V+SRFG RK +K+S E G+ L V+KPK+ +TL
Sbjct: 184 DV-------IPPPMMIESENLPPIEKPLVTSRFGHRKLMKASQEGGRALRVTKPKKNETL 243

Query: 250 ENTWRKITEGRSMPLTRHL-RKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRG 309
           ENTW+ ITEG+S PLTR L R+SDT+     +  +  V+    P   V KKS+TF++   
Sbjct: 244 ENTWKMITEGKSTPLTRQLYRRSDTF----GRGDSGGVDGEVKP---VYKKSDTFRDRTN 303

Query: 310 GGSPGGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 342
                 + ++++E S SQ+ELNRRVEAFIKKFN+EM+LQR ESL+QY+E+  RG
Sbjct: 304 YYQLAETAKVRKEPSLSQEELNRRVEAFIKKFNEEMKLQRMESLRQYKEITSRG 343

BLAST of Cucsat.G225 vs. TAIR 10
Match: AT5G54300.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-39
Identity = 135/336 (40.18%), Postives = 186/336 (55.36%), Query Frame = 0

Query: 18  LISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPYLYLLINCIIISIVASSK 77
           ++  G+ S+A  + L+VP ++ FV S  P I+ + +  L PPYLYL+IN II+ I+A+SK
Sbjct: 16  VVIAGVSSIATAMILTVPSVSHFVVSCFPIIYDNTVFLLKPPYLYLVINSIIVCIIATSK 75

Query: 78  L---QSNLENDPIPE--TPAPPPVTKISSDYAVCGSSDILNGYSSYNANQNVVTKVSDLE 137
           L    S++++  I E  TP P PV          G  ++++  S Y      V K+ D+ 
Sbjct: 76  LTHKSSSVDDSEISEVVTPIPIPVPVHLPSDIDSGYLNVVHVVSDYT---GFVEKIDDVS 135

Query: 138 IDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLPRKDSIGVLFSNK 197
           I+ + E   +  E  V E EK  E+         +  +    + N  P    I +L  + 
Sbjct: 136 INPTVEAIRKFPE--VQEAEKSKESSD-----SPEPETEKPKLKNDSP---EISIL-KHS 195

Query: 198 EEKPPVSSRFGQRKFVKSSPEG----KPLGVSK-PKRQDTLENTWRKITEGRSMPLTRHL 257
             KPP   RF Q+K +KS+ EG      LGV+K P+RQDTLE TW+KITEGRS PLT+HL
Sbjct: 196 TRKPP---RFNQQKSLKSNSEGGNKKTALGVTKPPRRQDTLETTWKKITEGRSTPLTKHL 255

Query: 258 RKSDTWE--SHGRKAPTMVVEDPATPPSKVMKKSETFKEGRGGGSPGGSGRMKREASPSQ 317
            KSDTW+  +H + +P            + M KSE  K+            +KRE SP Q
Sbjct: 256 TKSDTWQERAHVQSSP---------ENKEKMTKSENLKDINTPTEE--KTVLKREPSPGQ 315

Query: 318 DELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 342
           +ELNRRVEAFIKKFN+EMRLQR ESL +Y EMV  G
Sbjct: 316 EELNRRVEAFIKKFNEEMRLQRLESLAKYNEMVNGG 323

BLAST of Cucsat.G225 vs. TAIR 10
Match: AT1G11220.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 133.7 bits (335), Expect = 2.9e-31
Identity = 122/342 (35.67%), Postives = 172/342 (50.29%), Query Frame = 0

Query: 11  ILSLKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPYLYLLINCIII 70
           ++S+K  LI+ G+++++LFLK SVP+  DF  S  P  W+SFLSWL PPYL++ IN II 
Sbjct: 5   MISIKAALITAGIVAVSLFLKSSVPIAVDFSVSRFPIFWSSFLSWLKPPYLFVAINVIIT 64

Query: 71  SIVASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNANQNVVTKVS- 130
            I+ASSK   +     + E         +  +Y +                 NV+T+   
Sbjct: 65  IIMASSKFYQS-----VGEQDGEDDEILLGGEYTI----------------PNVITQAPP 124

Query: 131 ------DLEIDDSNEVYGRIEESRVSEMEKKGENDSMI---AMKGGDESSVLSSITNTLP 190
                 D + D    V   I  + V  +E   E   M       GGDE +V+ S  N   
Sbjct: 125 RRLVDLDADFDFVATVQSPILVAEVEILEVVFEEKEMAISGQTNGGDEFAVMRSELNQPI 184

Query: 191 RKDSIGVLFSNKEEKPPVSSRFGQRKFVKSSPEG-----KPLGVSKPKRQDTLENTWRKI 250
            ++S  +      EKP VS+R G RK +K+S +G     K L V KP R +TLENTW  I
Sbjct: 185 MEESENL---PPAEKPLVSARSGHRKPIKASSKGVNRKKKALKVVKPNRHETLENTWNMI 244

Query: 251 T-EGRSMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKE----GRGGGS 310
           T EG+S PLT H RK+           +M   +       V++K+ETF++     +   +
Sbjct: 245 TEEGKSTPLTCHYRKT-----------SMSGLNAGGDVKPVLRKAETFRDVTNYRQSSPT 304

Query: 311 PGGSGRMKREASPSQDELNRRVEAFIKKFNDEMRLQRQESLK 333
                +MK+E SPS++ELNRRVEAFIKK  +E    R ESLK
Sbjct: 305 VTSPVKMKKEMSPSREELNRRVEAFIKKCKEE----RLESLK 307

BLAST of Cucsat.G225 vs. TAIR 10
Match: AT1G11210.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 99.8 bits (247), Expect = 4.7e-21
Identity = 117/354 (33.05%), Postives = 172/354 (48.59%), Query Frame = 0

Query: 14  LKILLISTGLLSMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPYLYLLINCIIISIV 73
           +K +LISTG+++ A+ LK+ VP+  D  FS+ P I +SFL+WL PPYLY++ N III + 
Sbjct: 5   MKAVLISTGVVATAMHLKVIVPVAMD--FSQNPIILSSFLTWLKPPYLYVITNVIIIVVG 64

Query: 74  ASSKLQSNLENDPIPETPAPPPVTKISS-----DYAVCGSSDILNGYSSYNANQNVV--- 133
            S +                  +T +SS     DY    S D  N + +   +Q +V   
Sbjct: 65  VSYR------------------ITTVSSHVDGKDYEASYSGD--NKFQT--DHQQIVQEA 124

Query: 134 -----TKVSDLEIDDSNEVYGRIEESRVSEMEKKGENDSMIAMKGGDESSVLSSITNTLP 193
                T+  D +     +V   ++E  V   EK  E  + +  +      V+S   N  P
Sbjct: 125 PLRRRTETKDADFGFIGKVLQIVKEPEVVYEEK--ERPATVEEEEKKCIIVVSKSENQPP 184

Query: 194 RKDSIGVLFSNKEEKPPVSSRFGQRK-FVKSSP----EGKPLGVSKPKRQDTLENTWRKI 253
                        EKP V++R GQ+K  VK++P      + L V+KPKR +TLENTW+ I
Sbjct: 185 ------------VEKPLVTARIGQKKPVVKTTPAERNSMRALRVAKPKRNETLENTWKMI 244

Query: 254 TEGR--SMPLTRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKEGRG-----G 313
            EG   ++PLT + ++ DT+                T  S V+KKSETF +         
Sbjct: 245 MEGNKSTLPLTSYYKRPDTFGL-----------GEETKQSGVLKKSETFSDRTNCYQSLP 304

Query: 314 GSPGGSGRMKR-EASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEMVGRG 342
             P    ++K+ + S S+DELNR+VEAFIKK NDE    R  S+K   E+   G
Sbjct: 305 PPPPPLVKVKKVKVSRSRDELNRKVEAFIKKCNDE----RFASMKLDNEVARHG 305

BLAST of Cucsat.G225 vs. TAIR 10
Match: AT1G11230.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 106/340 (31.18%), Postives = 164/340 (48.24%), Query Frame = 0

Query: 14  LKILLISTGLL-SMALFLKLSVPLLADFVFSEIPSIWTSFLSWLTPPYLYLLINCIIISI 73
           +K +LISTG++ +M++FLK+ +P+   F  S   ++W+SFL WL PPYL++ +N +I  I
Sbjct: 7   IKAVLISTGIITAMSMFLKVFLPVTLYFSLS-FSTLWSSFLPWLKPPYLFVFVNVMITII 66

Query: 74  VASSKLQSNLENDPIPETPAPPPVTKISSDYAVCGSSDILNGYSSYNANQNVVTKVSDLE 133
           +ASS+   +     I +             Y    +  I+N  S          +V D++
Sbjct: 67  IASSRYYRS-----IGDHDGKDEKNLHGRGYYRIQTEPIVNQTSPPR------LEVKDMD 126

Query: 134 IDDSNEVYGRIEESRVSEME---KKGENDSMIAMKGGDESSVLSSITNTLPRKDSIGVLF 193
           +D   +    I+   V   E   ++ E +    + GGDE  VL    N  P         
Sbjct: 127 LDVDFDFMATIQPPEVERSEVVYEEKEEEISELINGGDEFVVLEESENLPP--------- 186

Query: 194 SNKEEKPPVSSRFGQRKFVKSSPEG-----KPLGVSKPKRQDTLENTWRKIT-EGRSMPL 253
               EKP VS+RF  RK VK +P+G     K L V  PKR    +N W+ I+ EG S PL
Sbjct: 187 ---VEKPLVSARFEHRKMVKVTPKGDDIRKKALKVVNPKR----DNKWKTISEEGTSRPL 246

Query: 254 -TRHLRKSDTWESHGRKAPTMVVEDPATPPSKVMKKSETFKE-----GRGGGSPGGSGRM 313
            T H ++ D +   G  A               ++KSETF++      +   +     +M
Sbjct: 247 STSHYQRPDIF---GLGA-----------GGDSLRKSETFRDVTNYYHQSSLTVTPPVKM 300

Query: 314 KREASPSQDELNRRVEAFIKKFNDEMRLQRQESLKQYQEM 338
           ++E  PS ++LNRR+EAFIKK  +E    R ESL+  +E+
Sbjct: 307 EKEMLPSLEDLNRRIEAFIKKVKEE----RLESLRLDKEV 300