Cucsat.G21615 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21615
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionrRNA adenine N(6)-methyltransferase
Locationctg949: 523364 .. 526898 (+)
RNA-Seq ExpressionCucsat.G21615
SyntenyCucsat.G21615
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAAATTTGGGCCTAAAAAAGTGTGGGATTCATTTGGGCTTAAAAAAGTGTGGGATTCATTTGGGCTGAAGTTATTTATACCTTTCACTGCAACTACTAGGGTTAGCCTTCAAATCTGAAGAAAGAGTTAAAGGAAACGGAAAAAGGAAATCAATTTTCCCCTTCCCACGCCCTCTCTCTCTCTCACGCAGCCCACGCCACAGTTTCCACCCAGCAGTCGCCGGCCAGCTTCCGGCAAACCACGCTGCCAGTAACCTGCTCCTCCTTCTCTTATCTTTGACTCTCTTCATAGTCAACAGCAGCAAGAACTCAGCGCCGCTCTGCTGACCATCGTTCCGTCATCCGTGACACTCCGACGACTAGTCGCTCGCCACTCCAACTTCCGGCCTCGGTCGCTCAAGCCATCTGTGGGCTTTCTTAGGGTGATTTTTTCTTACATTTGGCGCTGGAATTTTTCGTTACCCATTATATTTCTGGCCCCGTAACTGATTGCCCACTATGTGTTGTCCAGTTGTTTTAGTATTGAGTTTTATTTGGTGAGTTTTTGGTGAGAAATGCGGTTCCAATCTCTTCTTTAAGTTTCTGAGTAATACTATGTGATTTTAAGTTTTGGAATTGGATAATTTCAGCTTGTTTAATTAAAAAAAGGGTAACTTTGATTGTTTCTTATCCAATAGAAGAAAGGGTACCTTTGAGCCATAATTTATTAGGATAAGTTTTTTAGGCTTTACAGTAAATATACAGTCTCTATTTGGGTTTCAAACGTGGAAACTGATTGATGATTTTAATATAGAATTGATCTGAGACATTTGTAAGTCAAAGGAGAATTGTTTGTTTTCCTTGCTGCTTAGTAAATGTTTTAGAAAAGCTTGTATGCTAAATTTTACGAAACTTTATAAGCATGCTGTAGATATGAGTTTCCTGAGTTATTATGATAGGTATTTATGTTGCATGCTAGATTAGTTTAGTGGATACTAATATTTGGGAGGCAAGTGACATTATTATAGAATTCCTTAGAGTTGAGGAAAGATTTGTAGTAAATTAGTCATTGATTTGTTGCATGAATTATTTGTTTACATACTTCAAAATTTGTACCAATTTAGTTACTGTTGTGATAAATCTCATCAAAACTAAATGCTAATTTATATTACATAAACTAGTCAGTCTTTAAAGTTTATGAGCTAGTTTATTTGTAATCAACATATCAATCTATGCCTATAAAAAATCATTAGATCTTTATAATATTTGCTACGATAGGAATTAAATTGTTACCATTTGCAACTGGAGACTAAATTCTTATCATTTTGTGTATGGAGACTAAATAGAACCTAGAGTTGTAAATTGTTGCTTGTTTGAAAATTTGGAACTAAAATAATCTTTTAAACCTATTTTGTATTTAAAATTGAATAACTACTTTTTACGTTTGACTAAAATTTTAAGGGTATAAGTTTTTAAAACTTGTTAATATTCATGTGGTATTCATATGTATTTTTAATTAGTATATATCTGATCAATTTTATTCTAATTATCTATTAAAGAAACAGATATCATTTATATGTCTAATATATCGTGATACCTATTGATTCATATAATTTTTCAGATATTCCTTCATCGCCAACCTTTCCGAACAGAAGTTAGTTTGGAACAAATATTTGTTGAAGAAACCTTAGAATCTATTGTACTAGTTTCAATTTAGCAACAATGGCGGGAGGCAAGATTAGAAAAGAGAAGCCAAAAGCAGCGGCGAAGGCTGCAACACCGTATCAGGGAGGGATTTCATTCCACAAATCCAAGGGTCAGCATATTCTTAAGAATCCCTTGCTGGTTGACTCTATAGTTCAGAAATCTGGAATTAAGAGCACTGATGTGATCCTCGAGATTGGTCCTGGTACTGGTAATCTCACCAAGAAGCTGCTAGAATGTGGTAAGATGGTTATTGCTGTCGAGCTTGATCCTCGTATGGTTCTCGAGTTGCAGAGGCGTTTTCAAGACACTCCTTACTCTTCTCGCTTGAAGGTGAGTTTTCCCTTTGCTTTATGCTTTAGGTTTATCAAAATTTGAATTTGCTTTTATCTATTGATTCTTTATGTGTTTTTTCTTTATCCATCTGCCACCAATCATTTGGGATTTGTTTGATGTTTTGATTTAGTTGTTCTTATAGCTTCTGCTCGTTGTTAGGGATTGTTTGGTAGGCTGGAATGTAATGAGTTTGTAATGTTCTGTAATTTCATTACATTCCTTTCCCTCTATTTTTAATCCAACACTCTTTCACACATTTCTACCCTTCACGATCCCATTTGAACACCAATTTTAATCCTTGTTTTCAGTAATTTGATTACATGCTCGTCTTCCAAACAGGCTCCTATTTCTTTCAGTTGTATGAATTACTTGTACGTTGTACTCATATCTATCCTCTCTCTTTTGTAGTAGGCAAAATGGAAAATCTTTCCACTTTTTGAAATAGCTCATTGATTAGTTATATTTCCCATCAAATTCTCTTAACAACTATTTGAATGAACCAACCAGGTGTTGTTCTGTATTGGCAGATGTGTGCTTTTTCTTGACCTTTTGTTGGTGTTTTTACTTGATTGATATTTTGAGTTTCTCAAACTTGAATAATAATCGATCAGTTCTTCATCTTGATATGCTCAGGTTATTCAAGGAGATGTACTCAAGACTGAGCTTCCCTATTTTGATATATGTGTGGCAAATATTCCCTATCAAATATCATCACCCCTCACATTCAAATTGCTAAATCATCAACCTGCTTTTCGGTGTGCCATCATAATGTTCCAAAGGGAATTTGCTATGAGGTTAGTTGCTCAACCTGGAGACAAACTTTACTGCCGTTTGACCGTAAACACCCAACTCCTAGCTCGAGTGTCTCACCTCCTAAAAGTTGGAAAGAACAACTTTCGCCCTCCACCTAAGGTGGACTCATCTGTAGTTCGAATTGAGCCAAGGAAACCGCGTATTGAGGTGAAGCAGAAGGAGTGGGATGGATTTCTGAGAATCTGTTTCAATCGAAAGAACAAAACTCTTGGTTCAATATTTAGGCAGAAGAGTGTTCTTTCTTTGCTAGAGAAGAACTACAAGACATTGTGTTCACTTAACATCCTTCAGCAAGGTTCTGGAGGTAATGACGATAGTGCATTGAATTATTCAAATGAGGATCAAAGCATGGAAGTAGACGAGGATGGAGATGAGGAGGAGATGGATATGGAAGATGGTGATGCTGAAGAAGGGGAAGCTTCTGAATTTAAGGGTAAAGTGATGGGTGTTCTAAAGGAGGGAGATTTCGAAGAAAAGAGGTCTTCTAAACTCACCTTACAAGAGTTCTTATACTTGCTTTCTTTGTTCAACAAGGCTGGCATACATTTTTCCTGATTTTGTTTTTGTGATGTGTTTCTCCACAATATCCAATTTTGTCCTTCTCCCCTGTGTTTTAGGACTATCCTTTGTTTACTCCTAGAGAGTAGTAAAGTTATACCAATTTTTGTTGCTTCAACAACTGGCAATTGAAATTGTTACTCTTATTCTTATTCTTCTCTTTTTGTTGTATTAATCCAATGAATTTGTATTCTTTTATGATCCAAATCGTCA

Coding sequence (CDS)

ATGGCGGGAGGCAAGATTAGAAAAGAGAAGCCAAAAGCAGCGGCGAAGGCTGCAACACCGTATCAGGGAGGGATTTCATTCCACAAATCCAAGGGTCAGCATATTCTTAAGAATCCCTTGCTGGTTGACTCTATAGTTCAGAAATCTGGAATTAAGAGCACTGATGTGATCCTCGAGATTGGTCCTGGTACTGGTAATCTCACCAAGAAGCTGCTAGAATGTGGTAAGATGGTTATTGCTGTCGAGCTTGATCCTCGTATGGTTCTCGAGTTGCAGAGGCGTTTTCAAGACACTCCTTACTCTTCTCGCTTGAAGGTTATTCAAGGAGATGTACTCAAGACTGAGCTTCCCTATTTTGATATATGTGTGGCAAATATTCCCTATCAAATATCATCACCCCTCACATTCAAATTGCTAAATCATCAACCTGCTTTTCGGTGTGCCATCATAATGTTCCAAAGGGAATTTGCTATGAGGTTAGTTGCTCAACCTGGAGACAAACTTTACTGCCGTTTGACCGTAAACACCCAACTCCTAGCTCGAGTGTCTCACCTCCTAAAAGTTGGAAAGAACAACTTTCGCCCTCCACCTAAGGTGGACTCATCTGTAGTTCGAATTGAGCCAAGGAAACCGCGTATTGAGGTGAAGCAGAAGGAGTGGGATGGATTTCTGAGAATCTGTTTCAATCGAAAGAACAAAACTCTTGGTTCAATATTTAGGCAGAAGAGTGTTCTTTCTTTGCTAGAGAAGAACTACAAGACATTGTGTTCACTTAACATCCTTCAGCAAGGTTCTGGAGGTAATGACGATAGTGCATTGAATTATTCAAATGAGGATCAAAGCATGGAAGTAGACGAGGATGGAGATGAGGAGGAGATGGATATGGAAGATGGTGATGCTGAAGAAGGGGAAGCTTCTGAATTTAAGGGTAAAGTGATGGGTGTTCTAAAGGAGGGAGATTTCGAAGAAAAGAGGTCTTCTAAACTCACCTTACAAGAGTTCTTATACTTGCTTTCTTTGTTCAACAAGGCTGGCATACATTTTTCCTGA

Protein sequence

MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDAEEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Homology
BLAST of Cucsat.G21615 vs. ExPASy Swiss-Prot
Match: O22268 (Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN=DIM1A PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-144
Identity = 271/353 (76.77%), Postives = 307/353 (86.97%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKA-ATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILE 60
           MAGGKIRKEKPKA+ +A +  YQGGISFHKSKGQHILKNPLLVDSIVQK+GIKSTDVILE
Sbjct: 1   MAGGKIRKEKPKASNRAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILE 60

Query: 61  IGPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYF 120
           IGPGTGNLTKKLLE GK VIAVELD RMVLELQRRFQ TP+S+RLKVIQGDVLKTELP F
Sbjct: 61  IGPGTGNLTKKLLEAGKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRF 120

Query: 121 DICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL 180
           DICVANIPYQISSPLTFKLL H  +FRCA+IM+QREFAMRLVAQPGD LYCRL+VNTQL 
Sbjct: 121 DICVANIPYQISSPLTFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLY 180

Query: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIF 240
           ARVSHLLKVGKNNFRPPPKVDSSVVRIEPR+P  +V +KEWDGFLR+CF RKNKTLGSIF
Sbjct: 181 ARVSHLLKVGKNNFRPPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIF 240

Query: 241 RQKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDG---DEEEMDME 300
           +QKSVLS+LEKN+KTL ++    Q +G    +  +    DQSM +++D    D+++M+M+
Sbjct: 241 KQKSVLSMLEKNFKTLQAVLASLQNNGEPALNTTSMDLGDQSMGMEDDDNEMDDDDMEMD 300

Query: 301 DGDAEEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 350
           +G+ + GE SEFK KVM VLKEG FEEKRSSKL+ QEFLYLLSLFNK+GIHF+
Sbjct: 301 EGEGDGGETSEFKEKVMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHFT 353

BLAST of Cucsat.G21615 vs. ExPASy Swiss-Prot
Match: Q54QK7 (Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=dimt1 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 4.5e-93
Identity = 179/344 (52.03%), Postives = 233/344 (67.73%), Query Frame = 0

Query: 5   KIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGT 64
           K+ K K   AA+           +KS GQH+LKNPL++D+IV KS +KSTD +LEIGPGT
Sbjct: 12  KVEKTKSATAAR-----HHEFQMNKSYGQHLLKNPLIIDAIVDKSQLKSTDTVLEIGPGT 71

Query: 65  GNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVA 124
           GNLT KLLE  K VIA+E+DPRM  ELQ+R   +PY+  L++I GD LK +LPYFD+CVA
Sbjct: 72  GNLTMKLLENCKKVIAIEVDPRMAAELQKRVAASPYAQHLQIILGDFLKVDLPYFDVCVA 131

Query: 125 NIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSH 184
           N+PYQISSPLTFKLL H+P FR A++MFQ+EFA+RL A+PGD LYCRL+VNTQLL++V+H
Sbjct: 132 NVPYQISSPLTFKLLAHRPIFRTAVLMFQKEFALRLGAKPGDSLYCRLSVNTQLLSKVTH 191

Query: 185 LLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSV 244
           L+KVGKNNF PPPKV+S+VVRIEP  P   +   EWDG +++CF+RKNKTL  IFR  SV
Sbjct: 192 LMKVGKNNFLPPPKVESAVVRIEPFNPPPPINFVEWDGLVKLCFSRKNKTLSGIFRVSSV 251

Query: 245 LSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDAEEGE 304
           +  L +NYKT C+L                        +++ DG +E+M           
Sbjct: 252 IETLNQNYKTYCAL----------------------EGKMNTDGSDEQM----------- 311

Query: 305 ASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHF 349
               K  ++  L + DF + RSSKL + +FL LL+ F++ GIHF
Sbjct: 312 ----KELIIKTLTDNDFLDSRSSKLDINDFLKLLNKFHETGIHF 313

BLAST of Cucsat.G21615 vs. ExPASy Swiss-Prot
Match: Q9D0D4 (Probable dimethyladenosine transferase OS=Mus musculus OX=10090 GN=Dimt1 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.7e-93
Identity = 174/327 (53.21%), Postives = 223/327 (68.20%), Query Frame = 0

Query: 23  GGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPGTGNLTKKLLECGKMVIAVE 82
           GG+ F+   GQHILKNPL+V+SI+ K+ ++ TDV+LE+GPGTGN+T KLLE  K V+A E
Sbjct: 26  GGLMFNTGIGQHILKNPLIVNSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACE 85

Query: 83  LDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICVANIPYQISSPLTFKLLNHQ 142
           LDPR+V EL +R Q TP +S+L+V+ GDVLK++LP+FD CVAN+PYQISSP  FKLL H+
Sbjct: 86  LDPRLVAELHKRVQGTPLASKLQVLVGDVLKSDLPFFDACVANLPYQISSPFVFKLLLHR 145

Query: 143 PAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVSHLLKVGKNNFRPPPKVDSS 202
           P FRCAI+MFQREFA+RLVA+PGDKLYCRL++NTQLLARV HL+KVGKNNFRPPPKV+SS
Sbjct: 146 PFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESS 205

Query: 203 VVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKSVLSLLEKNYKTLCSLNILQ 262
           VVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  +V  LLEKNY+  CS+    
Sbjct: 206 VVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSV---- 265

Query: 263 QGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDAEEGEASEFKGKVMGVLKEGDFE 322
                            Q+  + ED                       K+  +L    F 
Sbjct: 266 -----------------QNTVIPED------------------FSIADKIQQILTSTGFS 313

Query: 323 EKRSSKLTLQEFLYLLSLFNKAGIHFS 350
           +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 326 DKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Cucsat.G21615 vs. ExPASy Swiss-Prot
Match: Q9UNQ2 (Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-92
Identity = 175/346 (50.58%), Postives = 230/346 (66.47%), Query Frame = 0

Query: 4   GKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPG 63
           G I + + +   +      GG+ F+   GQHILKNPL+++SI+ K+ ++ TDV+LE+GPG
Sbjct: 7   GAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPG 66

Query: 64  TGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICV 123
           TGN+T KLLE  K V+A ELDPR+V EL +R Q TP +S+L+V+ GDVLKT+LP+FD CV
Sbjct: 67  TGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCV 126

Query: 124 ANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVS 183
           AN+PYQISSP  FKLL H+P FRCAI+MFQREFA+RLVA+PGDKLYCRL++NTQLLARV 
Sbjct: 127 ANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVD 186

Query: 184 HLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKS 243
           HL+KVGKNNFRPPPKV+SSVVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  +
Sbjct: 187 HLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSA 246

Query: 244 VLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDAEEG 303
           V  LLEKNY+  CS++                     ++ + ED                
Sbjct: 247 VQQLLEKNYRIHCSVH---------------------NIIIPED---------------- 306

Query: 304 EASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 350
                  K+  +L    F +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 307 --FSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Cucsat.G21615 vs. ExPASy Swiss-Prot
Match: Q95KJ0 (Probable dimethyladenosine transferase OS=Macaca fascicularis OX=9541 GN=DIMT1 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.0e-92
Identity = 174/346 (50.29%), Postives = 230/346 (66.47%), Query Frame = 0

Query: 4   GKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEIGPG 63
           G I + + +   +      GG+ F+   GQHILKNPL+++SI+ K+ ++ TDV+LE+GPG
Sbjct: 7   GAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPG 66

Query: 64  TGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFDICV 123
           TGN+T KLLE  K V+A ELDPR+V EL++R Q TP +S+L+V+ GDVLKT+LP+FD CV
Sbjct: 67  TGNMTVKLLEKAKKVVACELDPRLVAELRKRVQGTPVASKLQVLVGDVLKTDLPFFDTCV 126

Query: 124 ANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLARVS 183
           AN+PYQISSP  FKLL H+P FRCAI+MFQRE A+RLVA+PGDKLYCRL++NTQLLARV 
Sbjct: 127 ANLPYQISSPFVFKLLLHRPFFRCAILMFQRELALRLVAKPGDKLYCRLSINTQLLARVD 186

Query: 184 HLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFRQKS 243
           HL+KVGKNNFRPPPKV+SSVVRIEP+ P   +  +EWDG +RI F RKNKTL + F+  +
Sbjct: 187 HLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSA 246

Query: 244 VLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDAEEG 303
           V  LLEKNY+  CS++                     ++ + ED                
Sbjct: 247 VQQLLEKNYRIHCSVH---------------------NIIIPED---------------- 306

Query: 304 EASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 350
                  K+  +L    F +KR+  + + +F+ LL  FN  GIHFS
Sbjct: 307 --FSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS 313

BLAST of Cucsat.G21615 vs. NCBI nr
Match: XP_004142540.1 (ribosomal RNA small subunit methyltransferase [Cucumis sativus] >XP_011660265.1 ribosomal RNA small subunit methyltransferase [Cucumis sativus] >KGN66758.1 hypothetical protein Csa_007400 [Cucumis sativus])

HSP 1 Score: 680 bits (1754), Expect = 2.73e-246
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDA 300
           QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSAL+YSNEDQSMEVDEDGDEEEMDMEDGDA
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALDYSNEDQSMEVDEDGDEEEMDMEDGDA 300

Query: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Cucsat.G21615 vs. NCBI nr
Match: XP_008443786.1 (PREDICTED: ribosomal RNA small subunit methyltransferase [Cucumis melo] >KAA0035147.1 ribosomal RNA small subunit methyltransferase [Cucumis melo var. makuwa] >TYK29222.1 ribosomal RNA small subunit methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 672 bits (1735), Expect = 2.15e-243
Identity = 343/349 (98.28%), Postives = 347/349 (99.43%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGKI++EKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIKREKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDA 300
           QKSVLSLLEKNYKTLCSLNILQQGS GNDDSA +YSNEDQSMEVDEDGD+EEMDMEDGDA
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSAGNDDSAFDYSNEDQSMEVDEDGDDEEMDMEDGDA 300

Query: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Cucsat.G21615 vs. NCBI nr
Match: XP_038877082.1 (ribosomal RNA small subunit methyltransferase [Benincasa hispida])

HSP 1 Score: 649 bits (1675), Expect = 3.76e-234
Identity = 334/355 (94.08%), Postives = 341/355 (96.06%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK++KEKPKAAAKAA PYQGGISFHKSKGQHILKNPLLVDSI+QKSGIKSTDVILEI
Sbjct: 1   MAGGKMKKEKPKAAAKAAAPYQGGISFHKSKGQHILKNPLLVDSIIQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDD------SALNYSNEDQSMEVDEDGDEEEMD 300
           QKSVLSLLEKNYKTLCSLNILQQ S GNDD      S    SNEDQ+MEVD+DGD+EEMD
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQSSVGNDDDGVFDYSRFGNSNEDQTMEVDDDGDDEEMD 300

Query: 301 MEDGDAEEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           MEDGD EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 MEDGDVEEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 355

BLAST of Cucsat.G21615 vs. NCBI nr
Match: XP_023525787.1 (ribosomal RNA small subunit methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622 bits (1605), Expect = 1.74e-223
Identity = 323/356 (90.73%), Postives = 337/356 (94.66%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+++EKPKA+AK A PYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKMKREKPKASAKTA-PYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLS 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYS-----NEDQSMEVDEDGDEEEMDM 300
           QKSVLSLLEKNYKTLC+LNI QQGS   DD   +YS     NEDQ+MEVD+DG +EEMDM
Sbjct: 241 QKSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNSNEDQTMEVDDDGGDEEMDM 300

Query: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           +D DAE  EGEASEFK KVMGVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DDSDAEGGEGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

BLAST of Cucsat.G21615 vs. NCBI nr
Match: XP_022155324.1 (ribosomal RNA small subunit methyltransferase [Momordica charantia])

HSP 1 Score: 622 bits (1605), Expect = 1.74e-223
Identity = 322/356 (90.45%), Postives = 337/356 (94.66%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+++EKPKAA K+ TPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKMKREKPKAATKS-TPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYS+RLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSTRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYS-----NEDQSMEVDEDGDEEEMDM 300
           QKSVL+LLEKNYKTLC+LN+ QQGS  NDD   +YS     NEDQ+MEVD+DG +EEMDM
Sbjct: 241 QKSVLTLLEKNYKTLCALNLPQQGSANNDDGDFDYSRFGDSNEDQTMEVDDDGADEEMDM 300

Query: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           +D DAE  EGEASEFK KV+ VLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 DDSDAEVGEGEASEFKNKVLSVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 355

BLAST of Cucsat.G21615 vs. ExPASy TrEMBL
Match: A0A0A0LY97 (rRNA adenine N(6)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G679560 PE=3 SV=1)

HSP 1 Score: 680 bits (1754), Expect = 1.32e-246
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDA 300
           QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSAL+YSNEDQSMEVDEDGDEEEMDMEDGDA
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALDYSNEDQSMEVDEDGDEEEMDMEDGDA 300

Query: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Cucsat.G21615 vs. ExPASy TrEMBL
Match: A0A5A7T0N9 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1228G00080 PE=3 SV=1)

HSP 1 Score: 672 bits (1735), Expect = 1.04e-243
Identity = 343/349 (98.28%), Postives = 347/349 (99.43%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGKI++EKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIKREKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDA 300
           QKSVLSLLEKNYKTLCSLNILQQGS GNDDSA +YSNEDQSMEVDEDGD+EEMDMEDGDA
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSAGNDDSAFDYSNEDQSMEVDEDGDDEEMDMEDGDA 300

Query: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Cucsat.G21615 vs. ExPASy TrEMBL
Match: A0A1S3B8X1 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487290 PE=3 SV=1)

HSP 1 Score: 672 bits (1735), Expect = 1.04e-243
Identity = 343/349 (98.28%), Postives = 347/349 (99.43%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGKI++EKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKIKREKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYSNEDQSMEVDEDGDEEEMDMEDGDA 300
           QKSVLSLLEKNYKTLCSLNILQQGS GNDDSA +YSNEDQSMEVDEDGD+EEMDMEDGDA
Sbjct: 241 QKSVLSLLEKNYKTLCSLNILQQGSAGNDDSAFDYSNEDQSMEVDEDGDDEEMDMEDGDA 300

Query: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 EEGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349

BLAST of Cucsat.G21615 vs. ExPASy TrEMBL
Match: A0A6J1DPX6 (rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111022457 PE=3 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 8.43e-224
Identity = 322/356 (90.45%), Postives = 337/356 (94.66%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+++EKPKAA K+ TPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKMKREKPKAATKS-TPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYS+RLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSTRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYS-----NEDQSMEVDEDGDEEEMDM 300
           QKSVL+LLEKNYKTLC+LN+ QQGS  NDD   +YS     NEDQ+MEVD+DG +EEMDM
Sbjct: 241 QKSVLTLLEKNYKTLCALNLPQQGSANNDDGDFDYSRFGDSNEDQTMEVDDDGADEEMDM 300

Query: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           +D DAE  EGEASEFK KV+ VLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS
Sbjct: 301 DDSDAEVGEGEASEFKNKVLSVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 355

BLAST of Cucsat.G21615 vs. ExPASy TrEMBL
Match: A0A6J1GBX4 (rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452763 PE=3 SV=1)

HSP 1 Score: 620 bits (1599), Expect = 6.92e-223
Identity = 321/356 (90.17%), Postives = 337/356 (94.66%), Query Frame = 0

Query: 1   MAGGKIRKEKPKAAAKAATPYQGGISFHKSKGQHILKNPLLVDSIVQKSGIKSTDVILEI 60
           MAGGK+++EKPKA+AK A PYQGGISFHKSKGQHILKNP+LVDSIVQKSGIKSTDVILEI
Sbjct: 1   MAGGKMKREKPKASAKTA-PYQGGISFHKSKGQHILKNPMLVDSIVQKSGIKSTDVILEI 60

Query: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYSSRLKVIQGDVLKTELPYFD 120
           GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPY+SRLKVIQGDVLKTELPYFD
Sbjct: 61  GPGTGNLTKKLLECGKMVIAVELDPRMVLELQRRFQDTPYASRLKVIQGDVLKTELPYFD 120

Query: 121 ICVANIPYQISSPLTFKLLNHQPAFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLA 180
           ICVANIPYQISSPLTFKLLNHQP+FRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLL+
Sbjct: 121 ICVANIPYQISSPLTFKLLNHQPSFRCAIIMFQREFAMRLVAQPGDKLYCRLTVNTQLLS 180

Query: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240
           RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR
Sbjct: 181 RVSHLLKVGKNNFRPPPKVDSSVVRIEPRKPRIEVKQKEWDGFLRICFNRKNKTLGSIFR 240

Query: 241 QKSVLSLLEKNYKTLCSLNILQQGSGGNDDSALNYS-----NEDQSMEVDEDGDEEEMDM 300
           QKSVLSLLEKNYKTLC+LNI QQGS   DD   +YS     NEDQ+MEVD+DG +EEMDM
Sbjct: 241 QKSVLSLLEKNYKTLCALNIPQQGSADKDDGDFDYSRFGNLNEDQTMEVDDDGGDEEMDM 300

Query: 301 EDGDAE--EGEASEFKGKVMGVLKEGDFEEKRSSKLTLQEFLYLLSLFNKAGIHFS 349
           +D DAE  +GEASEFK KVMGVLKEGDFEEKRSSKL+LQEFLYLLSLFNKAGIHFS
Sbjct: 301 DDSDAEGGDGEASEFKDKVMGVLKEGDFEEKRSSKLSLQEFLYLLSLFNKAGIHFS 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222681.1e-14476.77Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q54QK74.5e-9352.03Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=d... [more]
Q9D0D47.7e-9353.21Probable dimethyladenosine transferase OS=Mus musculus OX=10090 GN=Dimt1 PE=2 SV... [more]
Q9UNQ21.3e-9250.58Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=... [more]
Q95KJ05.0e-9250.29Probable dimethyladenosine transferase OS=Macaca fascicularis OX=9541 GN=DIMT1 P... [more]
Match NameE-valueIdentityDescription
XP_004142540.12.73e-24699.71ribosomal RNA small subunit methyltransferase [Cucumis sativus] >XP_011660265.1 ... [more]
XP_008443786.12.15e-24398.28PREDICTED: ribosomal RNA small subunit methyltransferase [Cucumis melo] >KAA0035... [more]
XP_038877082.13.76e-23494.08ribosomal RNA small subunit methyltransferase [Benincasa hispida][more]
XP_023525787.11.74e-22390.73ribosomal RNA small subunit methyltransferase [Cucurbita pepo subsp. pepo][more]
XP_022155324.11.74e-22390.45ribosomal RNA small subunit methyltransferase [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LY971.32e-24699.71rRNA adenine N(6)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G679560 P... [more]
A0A5A7T0N91.04e-24398.28rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3B8X11.04e-24398.28rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487290 PE=3... [more]
A0A6J1DPX68.43e-22490.45rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110224... [more]
A0A6J1GBX46.92e-22390.17rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11145276... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020598Ribosomal RNA adenine methylase transferase, N-terminalSMARTSM00650rADcneu6coord: 41..210
e-value: 3.7E-87
score: 305.5
NoneNo IPR availableGENE3D1.10.8.480coord: 213..349
e-value: 1.4E-37
score: 130.1
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 27..211
e-value: 1.8E-67
score: 228.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..303
NoneNo IPR availablePANTHERPTHR11727:SF7DIMETHYLADENOSINE TRANSFERASE-RELATEDcoord: 10..343
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 57..139
e-value: 2.02237E-9
score: 52.4323
IPR011530Ribosomal RNA adenine dimethylaseTIGRFAMTIGR00755TIGR00755coord: 26..255
e-value: 9.4E-76
score: 252.4
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPFAMPF00398RrnaADcoord: 26..253
e-value: 2.1E-63
score: 213.9
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPANTHERPTHR11727DIMETHYLADENOSINE TRANSFERASEcoord: 10..343
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPROSITEPS51689SAM_RNA_A_N6_MTcoord: 27..345
score: 64.942787
IPR020596Ribosomal RNA adenine methylase transferase, conserved sitePROSITEPS01131RRNA_A_DIMETHcoord: 57..84
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 22..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21615.T2Cucsat.G21615.T2mRNA
Cucsat.G21615.T1Cucsat.G21615.T1mRNA
Cucsat.G21615.T3Cucsat.G21615.T3mRNA
Cucsat.G21615.T4Cucsat.G21615.T4mRNA
Cucsat.G21615.T5Cucsat.G21615.T5mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0001708 cell fate specification
biological_process GO:0031167 rRNA methylation
biological_process GO:0000154 rRNA modification
biological_process GO:0006364 rRNA processing
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005730 nucleolus
molecular_function GO:0003723 RNA binding
molecular_function GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
molecular_function GO:0008649 rRNA methyltransferase activity