Cucsat.G21557 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21557
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPentatricopeptide repeat-containing protein
Locationctg944: 932650 .. 935176 (+)
RNA-Seq ExpressionCucsat.G21557
SyntenyCucsat.G21557
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAAACTCCCTCTAAAGTGTGACGCGGAACACGCCCTTAATCCAATCGGGCTGACATCGTGGGAGTTCTACGCTTTAAGCCATCCACAACAGTACAACACCGTGACTATCGAGCCCAATACTACCAATTCGTAGGCTTCAGTTCTTCCTTTTCTGGTTTGATGACGATCATACAAAACTTCTTGTGATTTCGTTCCCAAATGCTCCATTTCAAAAATTTCGCAACAGGGCTAATACGGTTCCGGTACATTTTTCTCAACCGCCATTTCAGTAACTCAAATTCTTTGGTGAATGGCTCCACCGCGCCTTCGAAAGACGACTATTTCGCTGCAATCCACCATATCTCCCACATTGTCCGCCGAGACTTCTACATGGAGCGCACTCTCAACAAGCTCCGAATCTCCAACCTCAATTCCGAGCTCGTTTTCAGAGTCCTTCGCGCTTGCTCCAACTCTGGTACCGAGTCCTTCCGTTTCTTCAACTGGGCTTGCTCTCACAACCCCTCTTACCAACCCACTACCCTTGAATTTGAAGAGCTCGTCAAAACCCTAGCTCGGACCAGAAAGTATACCACGATGTGGAAAGTTCTTCTTCAGATGAAGACTCAGAATCTCAAAATTTCACCGGAAACGATATCGTTCATAATTCAAGAGTATGGTAAGCAGGGCCTTGTAGATAATGCGGTTACCATATTCAATCAATGTTCCAAATCTATCGATTGTCCACAAACAGTTGAGGTCTATAACGCGTTACTTTTTGCGCTTTGTGAGGTTAAAATGTTTCATGGAGCTTATGCGTTGATTAGGAGGATGATTAGAAAAGGCGTAACTCCTGATAAAAAGACTTATGGAACTCTTGTAACTGGATGGTGCTCAGCGGGGAAGATGAAGGAAGCTCAGGAGTTCTTGGAGGAAATGAGCCAGAAGGGGTTCAATCCTCCTTTGCGAGGTCGTGATCTTTTGGTTGAAGGATTGCTTAATGCAGGGTATTTAGAATCTGCTAAGGATATGGTTAGAAAAATGACTAAAGAAGGATCTGTGCCTGATATAGGAACTTTTAATTCTCTGATTGATGTTATATGCAACTCTGGAGAAGTTGATTTTTGCATTAATATTTTTCATGAGGTGTGCAAGTTGGGGCTTTGTCCTGATATAAATACTTACAAGATTTTGATTCCAGCAACTTCGAAAGTAGGTAGGATTGATGAAGCATTCAGGCTTTTGCATTGCTGTATTGAGGATGGACATGTACCGTTTCCAAGTCTTTATGGACCAATACTTAAAGGAATGTGTAAAAGGGGTCAGTTCGATGATGCATTTTGCTTTTTTGGTGATATGAAACATAAGGGGCATCCACCAAATCGACCAGTGTACACAATGTTGATAACAATGTGTGGACGTGGAGGGAGATTTGTTGATGCTGCTAATTACTTGATGGAAATGGCTGAACTTGGTTTACCTCCAATTTCAAGGTGCTTTGATATGGTTACTGATGGATTGAAGAACTGTGGAAAACATGATTTAGCAAAGAAGATTGAACAGCTTGAAGTTTCTATTCGAGGCATTTGATCCTATAGTAAAGTAATAAGCAAGTACTTATTCTGCTTGAAGGATGTGGTTGAAATGGATATGAAAAACGAAAAACTACAGAAGTCGGAAAAAGGACAAACAAAGAAGCACTTCTCTTAGGAAATTCCTTCTAATCACTCTTACACATGTGGAGAGAGTTCAGAGAAAGGAAGTCTCAAGTTGAGGTTATCATCCAGCGAACGATCAACATTTTCAGATGCACTGTATGGATTGTGACTGAGGCTACTGCTATCTAGGTTTCATATACAGCTTCTAATGCTTAATTGCTAATATTATCTAACTGTTTAGTGCATGATTTGCTTGGTCATTTAATCCTGTCTCATGAAAACGTATATTTCCAATGTGCTATGGCCATTAAAATATTTGCTTTGGGTGCTGCCAACTATTGAGCTATGAACAGTATCCATTGAGTTGATAAGACCACACGGTCGATTCTTACAGTTTTCCGCTTTGTTTTTCTAAAAGTTAGAGTTACTATTTGGCTTGAGAAAGCTGAGAGGTGATTTCTACAGGGAAGTGCTGAATTCTTTGAGTTCAAAGGTGAATCGTTTTCTTGCTTCAAGGTGGCGTTCTCTGTCAAAGGATTGCAGTAGCCTTTTCTTCAAATCTATATCAATTGGATTCTTGTAATGTCTTCTGATTCCGGAAGGAAAGACGACTACTTCCAAGTCGATTTGAATTGGGGAGAGATTGTGCTCTTATAAAAGGGGGTTAACTGTAGACTCTCCTTAGCTTTCCATATTCTCATGTATTTCAATCCTGCGATCATTCAATCTTCAATTATATGCTTGTTTTTTCTTTGGTGAGTGATGTAGATATGGTATATGTTGGATATTATATTAAACCGGATGTCCTGGATTTAAGCTCTTGTTGTCTCTCAATTTAATAATGATTTTCACTTGTTTGGTCTTTTTGATATTTCAAACCCACAGGTGAGC

Coding sequence (CDS)

ATGCTCCATTTCAAAAATTTCGCAACAGGGCTAATACGGTTCCGGTACATTTTTCTCAACCGCCATTTCAGTAACTCAAATTCTTTGGTGAATGGCTCCACCGCGCCTTCGAAAGACGACTATTTCGCTGCAATCCACCATATCTCCCACATTGTCCGCCGAGACTTCTACATGGAGCGCACTCTCAACAAGCTCCGAATCTCCAACCTCAATTCCGAGCTCGTTTTCAGAGTCCTTCGCGCTTGCTCCAACTCTGGTACCGAGTCCTTCCGTTTCTTCAACTGGGCTTGCTCTCACAACCCCTCTTACCAACCCACTACCCTTGAATTTGAAGAGCTCGTCAAAACCCTAGCTCGGACCAGAAAGTATACCACGATGTGGAAAGTTCTTCTTCAGATGAAGACTCAGAATCTCAAAATTTCACCGGAAACGATATCGTTCATAATTCAAGAGTATGGTAAGCAGGGCCTTGTAGATAATGCGGTTACCATATTCAATCAATGTTCCAAATCTATCGATTGTCCACAAACAGTTGAGGTCTATAACGCGTTACTTTTTGCGCTTTGTGAGGTTAAAATGTTTCATGGAGCTTATGCGTTGATTAGGAGGATGATTAGAAAAGGCGTAACTCCTGATAAAAAGACTTATGGAACTCTTGTAACTGGATGGTGCTCAGCGGGGAAGATGAAGGAAGCTCAGGAGTTCTTGGAGGAAATGAGCCAGAAGGGGTTCAATCCTCCTTTGCGAGGTCGTGATCTTTTGGTTGAAGGATTGCTTAATGCAGGGTATTTAGAATCTGCTAAGGATATGGTTAGAAAAATGACTAAAGAAGGATCTGTGCCTGATATAGGAACTTTTAATTCTCTGATTGATGTTATATGCAACTCTGGAGAAGTTGATTTTTGCATTAATATTTTTCATGAGGTGTGCAAGTTGGGGCTTTGTCCTGATATAAATACTTACAAGATTTTGATTCCAGCAACTTCGAAAGTAGGTAGGATTGATGAAGCATTCAGGCTTTTGCATTGCTGTATTGAGGATGGACATGTACCGTTTCCAAGTCTTTATGGACCAATACTTAAAGGAATGTGTAAAAGGGGTCAGTTCGATGATGCATTTTGCTTTTTTGGTGATATGAAACATAAGGGGCATCCACCAAATCGACCAGTGTACACAATGTTGATAACAATGTGTGGACGTGGAGGGAGATTTGTTGATGCTGCTAATTACTTGATGGAAATGGCTGAACTTGGTTTACCTCCAATTTCAAGGTGCTTTGATATGGTTACTGATGGATTGAAGAACTGTGGAAAACATGATTTAGCAAAGAAGATTGAACAGCTTGAAGTTTCTATTCGAGGCATTTGA

Protein sequence

MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMERTLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI
Homology
BLAST of Cucsat.G21557 vs. ExPASy Swiss-Prot
Match: Q94JX6 (Pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g18390 PE=2 SV=2)

HSP 1 Score: 634.0 bits (1634), Expect = 1.3e-180
Identity = 302/435 (69.43%), Postives = 358/435 (82.30%), Query Frame = 0

Query: 21  RHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMERTLNKLRISNLNSELVFRVLR 80
           RHF++   L +  + P+K DYFAAI+H+ +IVRR+ + ER+LN LR+  + SE VFRVLR
Sbjct: 27  RHFNSLEPLQSSDSTPTKGDYFAAINHVVNIVRREIHPERSLNSLRLP-VTSEFVFRVLR 86

Query: 81  ACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQMKTQNLKI 140
           A S S  +S RFFNWA S NPSY PT++E+EEL K+LA  +KY +MWK+L QMK  +L I
Sbjct: 87  ATSRSSNDSLRFFNWARS-NPSYTPTSMEYEELAKSLASHKKYESMWKILKQMKDLSLDI 146

Query: 141 SPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMFHGAYAL 200
           S ET+ FII++YGK G VD AV +FN   K++ C QTV+VYN+LL ALC+VKMFHGAYAL
Sbjct: 147 SGETLCFIIEQYGKNGHVDQAVELFNGVPKTLGCQQTVDVYNSLLHALCDVKMFHGAYAL 206

Query: 201 IRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLN 260
           IRRMIRKG+ PDK+TY  LV GWCSAGKMKEAQEFL+EMS++GFNPP RGRDLL+EGLLN
Sbjct: 207 IRRMIRKGLKPDKRTYAILVNGWCSAGKMKEAQEFLDEMSRRGFNPPARGRDLLIEGLLN 266

Query: 261 AGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINT 320
           AGYLESAK+MV KMTK G VPDI TFN LI+ I  SGEV+FCI +++  CKLGLC DI+T
Sbjct: 267 AGYLESAKEMVSKMTKGGFVPDIQTFNILIEAISKSGEVEFCIEMYYTACKLGLCVDIDT 326

Query: 321 YKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMK 380
           YK LIPA SK+G+IDEAFRLL+ C+EDGH PFPSLY PI+KGMC+ G FDDAF FF DMK
Sbjct: 327 YKTLIPAVSKIGKIDEAFRLLNNCVEDGHKPFPSLYAPIIKGMCRNGMFDDAFSFFSDMK 386

Query: 381 HKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLPPISRCFDMVTDGLKNCGKHD 440
            K HPPNRPVYTMLITMCGRGG+FVDAANYL+EM E+GL PISRCFDMVTDGLKN GKHD
Sbjct: 387 VKAHPPNRPVYTMLITMCGRGGKFVDAANYLVEMTEMGLVPISRCFDMVTDGLKNGGKHD 446

Query: 441 LAKKIEQLEVSIRGI 456
           LA +IEQLEV +RG+
Sbjct: 447 LAMRIEQLEVQLRGV 459

BLAST of Cucsat.G21557 vs. ExPASy Swiss-Prot
Match: Q9FH87 (Putative pentatricopeptide repeat-containing protein At5g65820 OS=Arabidopsis thaliana OX=3702 GN=At5g65820 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.9e-41
Identity = 114/413 (27.60%), Postives = 200/413 (48.43%), Query Frame = 0

Query: 31  NGSTAPSKDDYFAAIHHISHIVRRDFY-----MERTLNKLRISNLNSELVFRVLRACSNS 90
           N  T  SK D FA+    S+ + R F+     +E  LN+  +  L   L+ RVL  C ++
Sbjct: 68  NDRTKNSKYDEFASDVEKSYRILRKFHSRVPKLELALNESGV-ELRPGLIERVLNRCGDA 127

Query: 91  GTESFRFFNWACSHNPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQMKTQNLK-ISPET 150
           G   +RFF WA +  P Y  +   ++ +VK L++ R++  +W ++ +M+ +N + I PE 
Sbjct: 128 GNLGYRFFVWA-AKQPRYCHSIEVYKSMVKILSKMRQFGAVWGLIEEMRKENPQLIEPEL 187

Query: 151 ISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRM 210
              ++Q +    +V  A+ + ++  K    P    V+  LL ALC+      A  L   M
Sbjct: 188 FVVLVQRFASADMVKKAIEVLDEMPKFGFEPDEY-VFGCLLDALCKHGSVKDAAKLFEDM 247

Query: 211 IRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYL 270
            R     + + + +L+ GWC  GKM EA+  L +M++ GF P +     L+ G  NAG +
Sbjct: 248 -RMRFPVNLRYFTSLLYGWCRVGKMMEAKYVLVQMNEAGFEPDIVDYTNLLSGYANAGKM 307

Query: 271 ESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKIL 330
             A D++R M + G  P+   +  LI  +C    ++  + +F E+ +     D+ TY  L
Sbjct: 308 ADAYDLLRDMRRRGFEPNANCYTVLIQALCKVDRMEEAMKVFVEMERYECEADVVTYTAL 367

Query: 331 IPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGH 390
           +    K G+ID+ + +L   I+ G +P    Y  I+    K+  F++       M+   +
Sbjct: 368 VSGFCKWGKIDKCYIVLDDMIKKGLMPSELTYMHIMVAHEKKESFEECLELMEKMRQIEY 427

Query: 391 PPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLPPISRCFDMVTDGLKNCG 438
            P+  +Y ++I +  + G   +A     EM E GL P    F ++ +GL + G
Sbjct: 428 HPDIGIYNVVIRLACKLGEVKEAVRLWNEMEENGLSPGVDTFVIMINGLASQG 476

BLAST of Cucsat.G21557 vs. ExPASy Swiss-Prot
Match: Q9S7R4 (Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OTP43 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.4e-38
Identity = 105/414 (25.36%), Postives = 184/414 (44.44%), Query Frame = 0

Query: 74  LVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQM 133
           LV  VL+   N G ++ +FF++  +H+  Y      F+  +   AR   + T+W ++ +M
Sbjct: 58  LVNSVLKRLWNHGPKALQFFHFLDNHHREYVHDASSFDLAIDIAARLHLHPTVWSLIHRM 117

Query: 134 KTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKM 193
           ++  +  SP+T + + + Y   G  D AV +F    +   C Q +  +N +L  LC+ K 
Sbjct: 118 RSLRIGPSPKTFAIVAERYASAGKPDKAVKLFLNMHEH-GCFQDLASFNTILDVLCKSKR 177

Query: 194 FHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDL 253
              AY L  R +R   + D  TY  ++ GWC   +  +A E L+EM ++G NP L   + 
Sbjct: 178 VEKAYELF-RALRGRFSVDTVTYNVILNGWCLIKRTPKALEVLKEMVERGINPNLTTYNT 237

Query: 254 LVEGLLNAGY-----------------------------------LESAKDMVRKMTKEG 313
           +++G   AG                                    ++ A+++  +M +EG
Sbjct: 238 MLKGFFRAGQIRHAWEFFLEMKKRDCEIDVVTYTTVVHGFGVAGEIKRARNVFDEMIREG 297

Query: 314 SVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAF 373
            +P + T+N++I V+C    V+  + +F E+ + G  P++ TY +LI      G      
Sbjct: 298 VLPSVATYNAMIQVLCKKDNVENAVVMFEEMVRRGYEPNVTTYNVLIRGLFHAGEFSRGE 357

Query: 374 RLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLIT-- 433
            L+     +G  P    Y  +++   +  + + A   F  M      PN   Y +LI+  
Sbjct: 358 ELMQRMENEGCEPNFQTYNMMIRYYSECSEVEKALGLFEKMGSGDCLPNLDTYNILISGM 417

Query: 434 -MCGRGGRFVDAANYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLE 450
            +  R    V A   L+EM E G  P    F+ V +GL   G    AK+I +L+
Sbjct: 418 FVRKRSEDMVVAGKLLLEMVERGFIPRKFTFNRVLNGLLLTGNQAFAKEILRLQ 469

BLAST of Cucsat.G21557 vs. ExPASy Swiss-Prot
Match: Q9SR00 (Pentatricopeptide repeat-containing protein At3g04760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g04760 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-36
Identity = 89/318 (27.99%), Postives = 150/318 (47.17%), Query Frame = 0

Query: 104 QPTTLEFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVT 163
           QP    +  L+    +  +     +VL +M++++      T + +I     +G +D A+ 
Sbjct: 155 QPDVFAYNALINGFCKMNRIDDATRVLDRMRSKDFSPDTVTYNIMIGSLCSRGKLDLALK 214

Query: 164 IFNQCSKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGW 223
           + NQ   S +C  TV  Y  L+ A         A  L+  M+ +G+ PD  TY T++ G 
Sbjct: 215 VLNQL-LSDNCQPTVITYTILIEATMLEGGVDEALKLMDEMLSRGLKPDMFTYNTIIRGM 274

Query: 224 CSAGKMKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDI 283
           C  G +  A E +  +  KG  P +   ++L+  LLN G  E  + ++ KM  E   P++
Sbjct: 275 CKEGMVDRAFEMVRNLELKGCEPDVISYNILLRALLNQGKWEEGEKLMTKMFSEKCDPNV 334

Query: 284 GTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHC 343
            T++ LI  +C  G+++  +N+   + + GL PD  +Y  LI A  + GR+D A   L  
Sbjct: 335 VTYSILITTLCRDGKIEEAMNLLKLMKEKGLTPDAYSYDPLIAAFCREGRLDVAIEFLET 394

Query: 344 CIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGR 403
            I DG +P    Y  +L  +CK G+ D A   FG +   G  PN   Y  + +     G 
Sbjct: 395 MISDGCLPDIVNYNTVLATLCKNGKADQALEIFGKLGEVGCSPNSSSYNTMFSALWSSGD 454

Query: 404 FVDAANYLMEMAELGLPP 422
            + A + ++EM   G+ P
Sbjct: 455 KIRALHMILEMMSNGIDP 471

BLAST of Cucsat.G21557 vs. ExPASy Swiss-Prot
Match: Q9LQ14 (Pentatricopeptide repeat-containing protein At1g62930, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g62930 PE=2 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.7e-36
Identity = 94/338 (27.81%), Postives = 164/338 (48.52%), Query Frame = 0

Query: 100 NPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVD 159
           N   +P  + +  L++ L    +++   ++L  M  + +  +  T S +I  + K+G + 
Sbjct: 283 NKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLV 342

Query: 160 NAVTIFNQCSK-SIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGT 219
            A  ++++  K SID    +  Y++L+   C       A  +   MI K   P+  TY T
Sbjct: 343 EAEKLYDEMIKRSID--PDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVTYNT 402

Query: 220 LVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEG 279
           L+ G+C A +++E  E   EMSQ+G        + L++GL  AG  + A+ + +KM  +G
Sbjct: 403 LIKGFCKAKRVEEGMELFREMSQRGLVGNTVTYNTLIQGLFQAGDCDMAQKIFKKMVSDG 462

Query: 280 SVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAF 339
             PDI T++ L+D +C  G+++  + +F  + K  + PDI TY I+I    K G++++ +
Sbjct: 463 VPPDIITYSILLDGLCKYGKLEKALVVFEYLQKSKMEPDIYTYNIMIEGMCKAGKVEDGW 522

Query: 340 RLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMC 399
            L       G  P   +Y  ++ G C++G  ++A   F +MK  G  PN   Y  LI   
Sbjct: 523 DLFCSLSLKGVKPNVIIYTTMISGFCRKGLKEEADALFREMKEDGTLPNSGTYNTLIRAR 582

Query: 400 GRGGRFVDAANYLMEMAELGL----PPISRCFDMVTDG 433
            R G    +A  + EM   G       IS   +M+ DG
Sbjct: 583 LRDGDKAASAELIKEMRSCGFVGDASTISMVINMLHDG 618

BLAST of Cucsat.G21557 vs. NCBI nr
Match: XP_004142520.1 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucumis sativus] >KGN66807.1 hypothetical protein Csa_007547 [Cucumis sativus])

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60
           MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER
Sbjct: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60

Query: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120
           TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART
Sbjct: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120

Query: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180
           RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV
Sbjct: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180

Query: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240
           YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS
Sbjct: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240

Query: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300
           QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD
Sbjct: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300

Query: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360
           FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL
Sbjct: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360

Query: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420
           KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP
Sbjct: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420

Query: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455
           PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI
Sbjct: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455

BLAST of Cucsat.G21557 vs. NCBI nr
Match: XP_008462724.1 (PREDICTED: pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucumis melo] >KAA0062598.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK29718.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 440/455 (96.70%), Postives = 445/455 (97.80%), Query Frame = 0

Query: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60
           MLHFKNFATG IRF YI L R FSNSNS VNGSTAPSKDDYFAAIHHISHIVRRDFYMER
Sbjct: 1   MLHFKNFATGAIRFHYISLIRCFSNSNSSVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60

Query: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120
           TLNKLRIS LNSELVFRVLRACSN GTESFRFFNWACSHNPSYQPTTLE EELVKTLART
Sbjct: 61  TLNKLRISYLNSELVFRVLRACSNCGTESFRFFNWACSHNPSYQPTTLELEELVKTLART 120

Query: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180
           RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV
Sbjct: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180

Query: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240
           YNALLFALCEVKMFHGAYALIRRMI+KGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS
Sbjct: 181 YNALLFALCEVKMFHGAYALIRRMIKKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240

Query: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300
           QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEG VPDIGTFNSLIDVICNSGEVD
Sbjct: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGCVPDIGTFNSLIDVICNSGEVD 300

Query: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360
           FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+CCIEDGHVPFPSLYGPI+
Sbjct: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNCCIEDGHVPFPSLYGPII 360

Query: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420
           KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP
Sbjct: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420

Query: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455
           PISRCFDMVTDGLK+CGKHDLAKKIE+LEVSIRGI
Sbjct: 421 PISRCFDMVTDGLKSCGKHDLAKKIEKLEVSIRGI 455

BLAST of Cucsat.G21557 vs. NCBI nr
Match: XP_038879743.1 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Benincasa hispida] >XP_038879744.1 pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Benincasa hispida] >XP_038879746.1 pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Benincasa hispida] >XP_038879747.1 pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Benincasa hispida])

HSP 1 Score: 874 bits (2259), Expect = 0.0
Identity = 421/441 (95.46%), Postives = 426/441 (96.60%), Query Frame = 0

Query: 15  RYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMERTLNKLRISNLNSEL 74
           +YI L RHFS SNS  NGS APSKDDYFAAIHHISHIVRRDFYMERTLNKLRIS LNSEL
Sbjct: 25  KYICLIRHFSTSNSSWNGSVAPSKDDYFAAIHHISHIVRRDFYMERTLNKLRISYLNSEL 84

Query: 75  VFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLARTRKYTTMWKVLLQMK 134
           VFRVLRACSNSGTESFRFFNWAC+HNPSYQPTTLEFEELVKTLART+KY TMWKVL QMK
Sbjct: 85  VFRVLRACSNSGTESFRFFNWACTHNPSYQPTTLEFEELVKTLARTKKYATMWKVLQQMK 144

Query: 135 TQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMF 194
            QNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMF
Sbjct: 145 MQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEVYNALLFALCEVKMF 204

Query: 195 HGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMSQKGFNPPLRGRDLL 254
           HGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKM+EAQEFLEEMSQKGFNPPLRGRDLL
Sbjct: 205 HGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMREAQEFLEEMSQKGFNPPLRGRDLL 264

Query: 255 VEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGL 314
           VEGLLNAGY ESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGL
Sbjct: 265 VEGLLNAGYFESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVDFCINIFHEVCKLGL 324

Query: 315 CPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPILKGMCKRGQFDDAFC 374
           CPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGH+PFPSLYGPILKGMCKR QFDDAFC
Sbjct: 325 CPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHIPFPSLYGPILKGMCKRSQFDDAFC 384

Query: 375 FFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLPPISRCFDMVTDGLK 434
           FF DMK KGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAE GLPPISRCFDMVTDGLK
Sbjct: 385 FFSDMKRKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAEFGLPPISRCFDMVTDGLK 444

Query: 435 NCGKHDLAKKIEQLEVSIRGI 455
           NCGKHDLAKKIEQLEVSIRGI
Sbjct: 445 NCGKHDLAKKIEQLEVSIRGI 465

BLAST of Cucsat.G21557 vs. NCBI nr
Match: XP_022988097.1 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucurbita maxima])

HSP 1 Score: 857 bits (2215), Expect = 9.24e-313
Identity = 416/466 (89.27%), Postives = 433/466 (92.92%), Query Frame = 0

Query: 1   MLHFKNFATGL------------IRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHI 60
           M HFK F T              IRF  IFL RHFS SNS  NGS  PSKDDYFAAIHHI
Sbjct: 1   MFHFKIFTTQFSIIPKLHRITDAIRFHNIFLIRHFSTSNSSSNGSGTPSKDDYFAAIHHI 60

Query: 61  SHIVRRDFYMERTLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTL 120
           S+IVRRD YMERTLNKLRIS LNSELVFRVLRACSNSGTESFRFFNWACS+NPSYQPTTL
Sbjct: 61  SNIVRRDIYMERTLNKLRISYLNSELVFRVLRACSNSGTESFRFFNWACSNNPSYQPTTL 120

Query: 121 EFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQC 180
           EFEELVKTLART+KYTTMWKVL QMKTQNLKISPETISF+I+EYGKQGLVD AVTIFNQC
Sbjct: 121 EFEELVKTLARTKKYTTMWKVLHQMKTQNLKISPETISFVIEEYGKQGLVDGAVTIFNQC 180

Query: 181 SKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGK 240
           SKSIDCPQTVEVYNALLFALCE+KMFHGAYALIRRMIRKGVTPDKKTYG LVTGWCS+GK
Sbjct: 181 SKSIDCPQTVEVYNALLFALCEIKMFHGAYALIRRMIRKGVTPDKKTYGILVTGWCSSGK 240

Query: 241 MKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNS 300
           MKEAQ+FLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAK+MVRKMTKEGSVPD+GTFNS
Sbjct: 241 MKEAQQFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKNMVRKMTKEGSVPDLGTFNS 300

Query: 301 LIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDG 360
           LI+VIC+SGEVDFCINI+HEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+ CIEDG
Sbjct: 301 LINVICDSGEVDFCINIYHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNYCIEDG 360

Query: 361 HVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420
           H+PFPSLYGPI+KGMCKRGQFDDAFCFF DMKHKGHPPNRPVYTMLITMCGRGGRFVDAA
Sbjct: 361 HIPFPSLYGPIIKGMCKRGQFDDAFCFFSDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420

Query: 421 NYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRG 454
           NYLMEMAE GLPPISRCFDMVTDGLK+CGKHDLAKKIEQLEVSIRG
Sbjct: 421 NYLMEMAEFGLPPISRCFDMVTDGLKSCGKHDLAKKIEQLEVSIRG 466

BLAST of Cucsat.G21557 vs. NCBI nr
Match: XP_022960812.1 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucurbita moschata])

HSP 1 Score: 854 bits (2207), Expect = 1.53e-311
Identity = 416/466 (89.27%), Postives = 432/466 (92.70%), Query Frame = 0

Query: 1   MLHFKNFATGL------------IRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHI 60
           M HFK FAT              IRF  IFL RHFS SNS  NGS  PSKDDYFAAIHHI
Sbjct: 1   MFHFKIFATQFSIIPKLHRITDAIRFHNIFLIRHFSTSNSSSNGSGTPSKDDYFAAIHHI 60

Query: 61  SHIVRRDFYMERTLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTL 120
           S+IVRRD YMERTLNKLRIS LNSELVFRVLRACSNSGTESFRFFNWACS+NPSYQPTTL
Sbjct: 61  SNIVRRDIYMERTLNKLRISYLNSELVFRVLRACSNSGTESFRFFNWACSNNPSYQPTTL 120

Query: 121 EFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQC 180
           EFEELVKTLART+KYTTMWKVL QMKTQNLKISPETISF+I+EYGKQGLVD AVTIFNQC
Sbjct: 121 EFEELVKTLARTKKYTTMWKVLHQMKTQNLKISPETISFVIEEYGKQGLVDGAVTIFNQC 180

Query: 181 SKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGK 240
           SKSIDCPQTVEVYNALLFALCE+KMFHGAYALIRRMIRKGVTPDKKTYG LVTGWCS+GK
Sbjct: 181 SKSIDCPQTVEVYNALLFALCEIKMFHGAYALIRRMIRKGVTPDKKTYGILVTGWCSSGK 240

Query: 241 MKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNS 300
           MKEAQ+FLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAK+MVRKM KEGSVPD+GTFNS
Sbjct: 241 MKEAQQFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKNMVRKMIKEGSVPDLGTFNS 300

Query: 301 LIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDG 360
           LI+VICNSGEVDFCINI+HEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+ CIEDG
Sbjct: 301 LINVICNSGEVDFCINIYHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNYCIEDG 360

Query: 361 HVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420
           H+PFPSLYGPI+KGMCKRGQFDDAFCFF DMK KGHPPNRPVYTMLITMCGRGGRFVDAA
Sbjct: 361 HIPFPSLYGPIIKGMCKRGQFDDAFCFFSDMKLKGHPPNRPVYTMLITMCGRGGRFVDAA 420

Query: 421 NYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRG 454
           NYLMEMAE GLPPISRCFDMVTDGLK+CGKHDLAKKIEQLEVSIRG
Sbjct: 421 NYLMEMAEFGLPPISRCFDMVTDGLKSCGKHDLAKKIEQLEVSIRG 466

BLAST of Cucsat.G21557 vs. ExPASy TrEMBL
Match: A0A0A0M105 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695420 PE=4 SV=1)

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60
           MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER
Sbjct: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60

Query: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120
           TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART
Sbjct: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120

Query: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180
           RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV
Sbjct: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180

Query: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240
           YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS
Sbjct: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240

Query: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300
           QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD
Sbjct: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300

Query: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360
           FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL
Sbjct: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360

Query: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420
           KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP
Sbjct: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420

Query: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455
           PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI
Sbjct: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455

BLAST of Cucsat.G21557 vs. ExPASy TrEMBL
Match: A0A5A7V4T7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3607G00300 PE=4 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 440/455 (96.70%), Postives = 445/455 (97.80%), Query Frame = 0

Query: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60
           MLHFKNFATG IRF YI L R FSNSNS VNGSTAPSKDDYFAAIHHISHIVRRDFYMER
Sbjct: 1   MLHFKNFATGAIRFHYISLIRCFSNSNSSVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60

Query: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120
           TLNKLRIS LNSELVFRVLRACSN GTESFRFFNWACSHNPSYQPTTLE EELVKTLART
Sbjct: 61  TLNKLRISYLNSELVFRVLRACSNCGTESFRFFNWACSHNPSYQPTTLELEELVKTLART 120

Query: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180
           RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV
Sbjct: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180

Query: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240
           YNALLFALCEVKMFHGAYALIRRMI+KGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS
Sbjct: 181 YNALLFALCEVKMFHGAYALIRRMIKKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240

Query: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300
           QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEG VPDIGTFNSLIDVICNSGEVD
Sbjct: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGCVPDIGTFNSLIDVICNSGEVD 300

Query: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360
           FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+CCIEDGHVPFPSLYGPI+
Sbjct: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNCCIEDGHVPFPSLYGPII 360

Query: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420
           KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP
Sbjct: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420

Query: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455
           PISRCFDMVTDGLK+CGKHDLAKKIE+LEVSIRGI
Sbjct: 421 PISRCFDMVTDGLKSCGKHDLAKKIEKLEVSIRGI 455

BLAST of Cucsat.G21557 vs. ExPASy TrEMBL
Match: A0A1S3CJ74 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103501022 PE=4 SV=1)

HSP 1 Score: 910 bits (2351), Expect = 0.0
Identity = 440/455 (96.70%), Postives = 445/455 (97.80%), Query Frame = 0

Query: 1   MLHFKNFATGLIRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60
           MLHFKNFATG IRF YI L R FSNSNS VNGSTAPSKDDYFAAIHHISHIVRRDFYMER
Sbjct: 1   MLHFKNFATGAIRFHYISLIRCFSNSNSSVNGSTAPSKDDYFAAIHHISHIVRRDFYMER 60

Query: 61  TLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTLEFEELVKTLART 120
           TLNKLRIS LNSELVFRVLRACSN GTESFRFFNWACSHNPSYQPTTLE EELVKTLART
Sbjct: 61  TLNKLRISYLNSELVFRVLRACSNCGTESFRFFNWACSHNPSYQPTTLELEELVKTLART 120

Query: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180
           RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV
Sbjct: 121 RKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQCSKSIDCPQTVEV 180

Query: 181 YNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240
           YNALLFALCEVKMFHGAYALIRRMI+KGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS
Sbjct: 181 YNALLFALCEVKMFHGAYALIRRMIKKGVTPDKKTYGTLVTGWCSAGKMKEAQEFLEEMS 240

Query: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNSLIDVICNSGEVD 300
           QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEG VPDIGTFNSLIDVICNSGEVD
Sbjct: 241 QKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGCVPDIGTFNSLIDVICNSGEVD 300

Query: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDGHVPFPSLYGPIL 360
           FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+CCIEDGHVPFPSLYGPI+
Sbjct: 301 FCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNCCIEDGHVPFPSLYGPII 360

Query: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420
           KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP
Sbjct: 361 KGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAANYLMEMAELGLP 420

Query: 421 PISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRGI 455
           PISRCFDMVTDGLK+CGKHDLAKKIE+LEVSIRGI
Sbjct: 421 PISRCFDMVTDGLKSCGKHDLAKKIEKLEVSIRGI 455

BLAST of Cucsat.G21557 vs. ExPASy TrEMBL
Match: A0A6J1JC50 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111485446 PE=4 SV=1)

HSP 1 Score: 857 bits (2215), Expect = 4.48e-313
Identity = 416/466 (89.27%), Postives = 433/466 (92.92%), Query Frame = 0

Query: 1   MLHFKNFATGL------------IRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHI 60
           M HFK F T              IRF  IFL RHFS SNS  NGS  PSKDDYFAAIHHI
Sbjct: 1   MFHFKIFTTQFSIIPKLHRITDAIRFHNIFLIRHFSTSNSSSNGSGTPSKDDYFAAIHHI 60

Query: 61  SHIVRRDFYMERTLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTL 120
           S+IVRRD YMERTLNKLRIS LNSELVFRVLRACSNSGTESFRFFNWACS+NPSYQPTTL
Sbjct: 61  SNIVRRDIYMERTLNKLRISYLNSELVFRVLRACSNSGTESFRFFNWACSNNPSYQPTTL 120

Query: 121 EFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQC 180
           EFEELVKTLART+KYTTMWKVL QMKTQNLKISPETISF+I+EYGKQGLVD AVTIFNQC
Sbjct: 121 EFEELVKTLARTKKYTTMWKVLHQMKTQNLKISPETISFVIEEYGKQGLVDGAVTIFNQC 180

Query: 181 SKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGK 240
           SKSIDCPQTVEVYNALLFALCE+KMFHGAYALIRRMIRKGVTPDKKTYG LVTGWCS+GK
Sbjct: 181 SKSIDCPQTVEVYNALLFALCEIKMFHGAYALIRRMIRKGVTPDKKTYGILVTGWCSSGK 240

Query: 241 MKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNS 300
           MKEAQ+FLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAK+MVRKMTKEGSVPD+GTFNS
Sbjct: 241 MKEAQQFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKNMVRKMTKEGSVPDLGTFNS 300

Query: 301 LIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDG 360
           LI+VIC+SGEVDFCINI+HEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+ CIEDG
Sbjct: 301 LINVICDSGEVDFCINIYHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNYCIEDG 360

Query: 361 HVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420
           H+PFPSLYGPI+KGMCKRGQFDDAFCFF DMKHKGHPPNRPVYTMLITMCGRGGRFVDAA
Sbjct: 361 HIPFPSLYGPIIKGMCKRGQFDDAFCFFSDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420

Query: 421 NYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRG 454
           NYLMEMAE GLPPISRCFDMVTDGLK+CGKHDLAKKIEQLEVSIRG
Sbjct: 421 NYLMEMAEFGLPPISRCFDMVTDGLKSCGKHDLAKKIEQLEVSIRG 466

BLAST of Cucsat.G21557 vs. ExPASy TrEMBL
Match: A0A6J1H8G8 (pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461504 PE=4 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 7.40e-312
Identity = 416/466 (89.27%), Postives = 432/466 (92.70%), Query Frame = 0

Query: 1   MLHFKNFATGL------------IRFRYIFLNRHFSNSNSLVNGSTAPSKDDYFAAIHHI 60
           M HFK FAT              IRF  IFL RHFS SNS  NGS  PSKDDYFAAIHHI
Sbjct: 1   MFHFKIFATQFSIIPKLHRITDAIRFHNIFLIRHFSTSNSSSNGSGTPSKDDYFAAIHHI 60

Query: 61  SHIVRRDFYMERTLNKLRISNLNSELVFRVLRACSNSGTESFRFFNWACSHNPSYQPTTL 120
           S+IVRRD YMERTLNKLRIS LNSELVFRVLRACSNSGTESFRFFNWACS+NPSYQPTTL
Sbjct: 61  SNIVRRDIYMERTLNKLRISYLNSELVFRVLRACSNSGTESFRFFNWACSNNPSYQPTTL 120

Query: 121 EFEELVKTLARTRKYTTMWKVLLQMKTQNLKISPETISFIIQEYGKQGLVDNAVTIFNQC 180
           EFEELVKTLART+KYTTMWKVL QMKTQNLKISPETISF+I+EYGKQGLVD AVTIFNQC
Sbjct: 121 EFEELVKTLARTKKYTTMWKVLHQMKTQNLKISPETISFVIEEYGKQGLVDGAVTIFNQC 180

Query: 181 SKSIDCPQTVEVYNALLFALCEVKMFHGAYALIRRMIRKGVTPDKKTYGTLVTGWCSAGK 240
           SKSIDCPQTVEVYNALLFALCE+KMFHGAYALIRRMIRKGVTPDKKTYG LVTGWCS+GK
Sbjct: 181 SKSIDCPQTVEVYNALLFALCEIKMFHGAYALIRRMIRKGVTPDKKTYGILVTGWCSSGK 240

Query: 241 MKEAQEFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKDMVRKMTKEGSVPDIGTFNS 300
           MKEAQ+FLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAK+MVRKM KEGSVPD+GTFNS
Sbjct: 241 MKEAQQFLEEMSQKGFNPPLRGRDLLVEGLLNAGYLESAKNMVRKMIKEGSVPDLGTFNS 300

Query: 301 LIDVICNSGEVDFCINIFHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLHCCIEDG 360
           LI+VICNSGEVDFCINI+HEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLL+ CIEDG
Sbjct: 301 LINVICNSGEVDFCINIYHEVCKLGLCPDINTYKILIPATSKVGRIDEAFRLLNYCIEDG 360

Query: 361 HVPFPSLYGPILKGMCKRGQFDDAFCFFGDMKHKGHPPNRPVYTMLITMCGRGGRFVDAA 420
           H+PFPSLYGPI+KGMCKRGQFDDAFCFF DMK KGHPPNRPVYTMLITMCGRGGRFVDAA
Sbjct: 361 HIPFPSLYGPIIKGMCKRGQFDDAFCFFSDMKLKGHPPNRPVYTMLITMCGRGGRFVDAA 420

Query: 421 NYLMEMAELGLPPISRCFDMVTDGLKNCGKHDLAKKIEQLEVSIRG 454
           NYLMEMAE GLPPISRCFDMVTDGLK+CGKHDLAKKIEQLEVSIRG
Sbjct: 421 NYLMEMAEFGLPPISRCFDMVTDGLKSCGKHDLAKKIEQLEVSIRG 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94JX61.3e-18069.43Pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Arabidop... [more]
Q9FH878.9e-4127.60Putative pentatricopeptide repeat-containing protein At5g65820 OS=Arabidopsis th... [more]
Q9S7R41.4e-3825.36Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidop... [more]
Q9SR001.3e-3627.99Pentatricopeptide repeat-containing protein At3g04760, chloroplastic OS=Arabidop... [more]
Q9LQ141.7e-3627.81Pentatricopeptide repeat-containing protein At1g62930, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_004142520.10.0100.00pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucumis sa... [more]
XP_008462724.10.096.70PREDICTED: pentatricopeptide repeat-containing protein At5g18390, mitochondrial ... [more]
XP_038879743.10.095.46pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Benincasa ... [more]
XP_022988097.19.24e-31389.27pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucurbita ... [more]
XP_022960812.11.53e-31189.27pentatricopeptide repeat-containing protein At5g18390, mitochondrial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
A0A0A0M1050.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G695420 PE=4 SV=1[more]
A0A5A7V4T70.096.70Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CJ740.096.70pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucumis ... [more]
A0A6J1JC504.48e-31389.27pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucurbit... [more]
A0A6J1H8G87.40e-31289.27pentatricopeptide repeat-containing protein At5g18390, mitochondrial OS=Cucurbit... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 169..242
e-value: 5.6E-18
score: 67.1
coord: 243..308
e-value: 4.9E-9
score: 38.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 309..452
e-value: 3.0E-27
score: 97.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 40..168
e-value: 6.3E-8
score: 34.1
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 358..398
e-value: 4.4E-9
score: 36.4
coord: 281..327
e-value: 4.2E-8
score: 33.3
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 180..212
e-value: 3.6E-6
score: 24.8
coord: 390..421
e-value: 5.6E-4
score: 17.9
coord: 356..387
e-value: 2.7E-7
score: 28.3
coord: 254..283
e-value: 0.0022
score: 16.0
coord: 285..318
e-value: 7.1E-5
score: 20.7
coord: 215..246
e-value: 1.2E-4
score: 20.0
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 254..278
e-value: 1.0
score: 9.7
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 207..240
e-value: 8.1E-11
score: 41.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 387..421
score: 10.05157
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 212..246
score: 12.737088
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 352..386
score: 10.424253
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 317..351
score: 9.700809
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 177..211
score: 10.840783
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 282..316
score: 10.161182
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 20..454
NoneNo IPR availablePANTHERPTHR45613:SF30PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MITOCHONDRIALcoord: 20..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21557.T1Cucsat.G21557.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding