Cucsat.G21544 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21544
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	RPM1-interacting protein 4-like
Location	ctg944: 762556 .. 763722 (+)
RNA-Seq Expression	Cucsat.G21544
Synteny	Cucsat.G21544

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACAAAGTAAAAGCCAAAACACACTAAACACATCGAAAACAAACTTAGAAACTATCGAACTATAATTGACTGATCAAGAACAAAACTTCCAACCTCATTACTCCTCCATCTTTTTTTCTTCTAACAATACGAACTCTTGCATACTCCGCCGTTCCCGTCATCGCGTTGCAGCAACCACGGAGCTGCCACAGCTCATGGCAGTGAGTGTTTTCTTCTTCTTCTTTGTTGCTTTTTTTTTCTTTTTATCCATCAACACAAAAGCACAAATAAAACCCAACTATTAAATGATATCGTGGTCGTGTGGAATTATTAGTTAGAAGTAAGTTTGTATTAGTCTTCAATTAATTTAATGTAATGGGCAGGGGCCAAAGTCGTCATCTCACGTTCCCAAATTCGGTAATTGGGATTCCGATGACGTACCGTACACCATCTATTTCGAAAACGCCCGTGAACTCCAAGCCGCCGGCATCACCTTCGATCCCAACGATCCCGACACCTACCCGCCGACCGCCGTCGCAAACCAAAATTCAAGCCCAGCCAACCACCACCGTTATCACGATGGCGACGACTCCCACCAGCGGCGAGAGATTGTGAACTCCGGGTCGGAGAAATTAAGCAGTGAGAGAAGCGATTCGGATTACGCGCTTCTGAAAGCGAAACAGAGTGGGAGGAAGAAGAAAAATAGCAATGGAGCGGAAGGAATGAGTAGATTTTCTCCGGCGACGGTGGACGGAAAGGGTAATCGGAACCCTAGGGTGCGGCGGAATAATGAGGGGGAAATGATGGCGTCGGTGCCGAAATTTGGAAGCTGGGATGTGAGGGATCCGAAATCGGGAGATGGATATACGGCGATATTCAATAAAGTGAAGATAGAGAAGCAAGTTGGAGGTTCTAATGATACTCAGAGTGTTCCGGCATTGATGAATCAAACGAAGCAGCCAATTACTCAAACAACTGTTGGTAAAAGTTCAAGTCATGGATCCACTTCATTTGGTTCCAAGGTACCCTCCTCTTTGTTCTTGTTTCTTTCTCAAGTTTCTATTAATTTAGGTCATGTTTGTTAATTATTTGGTCTCCATTCATAACTGTTTTTCTATATCAATCACATCTTTGTTGAAATCAATATTTGTTTATTTATGCAGATATGCTGTTGTTTATATCCAAAGTGAAGCACATCGCACATCATGACTGCTTCAAGAACAGACTCAAAACGGTGCAAATGTATTTGCTCATTTTCACACTTTTATAAAATCTATATCCCTTCTTGTGTTTTGATATAAATAATATCTTTCATAATTTCAATAATTATATTTATCTTCAATTATAAATACAAATTATTATAATGGAATAAACCCAATCTAGAATTTGCTTGCTTCATTTTCC

Coding sequence (CDS)

ATGGCAGGGCCAAAGTCGTCATCTCACGTTCCCAAATTCGGTAATTGGGATTCCGATGACGTACCGTACACCATCTATTTCGAAAACGCCCGTGAACTCCAAGCCGCCGGCATCACCTTCGATCCCAACGATCCCGACACCTACCCGCCGACCGCCGTCGCAAACCAAAATTCAAGCCCAGCCAACCACCACCGTTATCACGATGGCGACGACTCCCACCAGCGGCGAGAGATTGTGAACTCCGGGTCGGAGAAATTAAGCAGTGAGAGAAGCGATTCGGATTACGCGCTTCTGAAAGCGAAACAGAGTGGGAGGAAGAAGAAAAATAGCAATGGAGCGGAAGGAATGAGTAGATTTTCTCCGGCGACGGTGGACGGAAAGGGTAATCGGAACCCTAGGGTGCGGCGGAATAATGAGGGGGAAATGATGGCGTCGGTGCCGAAATTTGGAAGCTGGGATGTGAGGGATCCGAAATCGGGAGATGGATATACGGCGATATTCAATAAAGTGAAGATAGAGAAGCAAGTTGGAGGTTCTAATGATACTCAGAGTGTTCCGGCATTGATGAATCAAACGAAGCAGCCAATTACTCAAACAACTGTTGGTAAAAGTTCAAGTCATGGATCCACTTCATTTGGTTCCAAGATATGCTGTTGTTTATATCCAAAGTGA

Protein sequence

MAGPKSSSHVPKFGNWDSDDVPYTIYFENARELQAAGITFDPNDPDTYPPTAVANQNSSPANHHRYHDGDDSHQRREIVNSGSEKLSSERSDSDYALLKAKQSGRKKKNSNGAEGMSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQVGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK

Homology

[Close]

BLAST of Cucsat.G21544 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.7e-06
Identity = 28/74 (37.84%), Postives = 38/74 (51.35%), Query Frame = 0


Query: 10  DGKGNRNPR---VRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQVGGSND 69
           DG G   P+   +R +   E +  VPKFG WD  +P S DGYT IFNKV+ E+  G +  
Sbjct: 124 DGTGKSRPKPTNLRADESPEKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVS 183

Query: 70  TQSVPALMNQTKQP 81
             S       ++ P
Sbjct: 184 GSSRTPTHQSSRNP 197

[Close]

BLAST of Cucsat.G21544 vs. ExPASy Swiss-Prot
Match: O22633 (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 6.4e-06
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = 0


Query: 31 VPKFGSWDVRDPKSGDGYTAIFNKVKIEKQVGG 64
          +PKFG WDV DP S +G+T IFNK + EK+ GG
Sbjct: 8  LPKFGEWDVNDPASAEGFTVIFNKARDEKKTGG 40

[Close]

BLAST of Cucsat.G21544 vs. NCBI nr
Match: XP_011660296.1 (RPM1-interacting protein 4 [Cucumis sativus] >KGN66824.1 hypothetical protein Csa_007241 [Cucumis sativus])

HSP 1 Score: 225 bits (574), Expect = 9.70e-73
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK 108
           VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK
Sbjct: 176 VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK 223

[Close]

BLAST of Cucsat.G21544 vs. NCBI nr
Match: XP_008458983.1 (PREDICTED: RPM1-interacting protein 4-like [Cucumis melo])

HSP 1 Score: 198 bits (503), Expect = 6.04e-62
Identity = 94/107 (87.85%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSP TVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPTTVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYP 107
           +GGSN+ Q+VP LMNQTKQP+TQTTV K+S+ GSTSF SKICCCL P
Sbjct: 176 IGGSNNPQTVPPLMNQTKQPMTQTTVAKTSTQGSTSFVSKICCCLGP 222

[Close]

BLAST of Cucsat.G21544 vs. NCBI nr
Match: KAA0054298.1 (RPM1-interacting protein 4-like [Cucumis melo var. makuwa] >TYK12335.1 RPM1-interacting protein 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 185 bits (469), Expect = 1.35e-56
Identity = 88/101 (87.13%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSP TVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPTTVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKI 101
           +GGSN+ Q+VP LMNQTKQP+TQTTV K+S+ GSTSF SK+
Sbjct: 176 IGGSNNPQTVPPLMNQTKQPMTQTTVAKTSTQGSTSFVSKV 216

[Close]

BLAST of Cucsat.G21544 vs. NCBI nr
Match: XP_038895249.1 (RPM1-interacting protein 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 172 bits (435), Expect = 1.27e-51
Identity = 87/113 (76.99%), Postives = 96/113 (84.96%), Query Frame = 0


Query: 1   MSRFSPATV----DGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK 60
           MSRF+P T      GKGNRNPRVRRN+EGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK
Sbjct: 112 MSRFTPTTTVSGHGGKGNRNPRVRRNDEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK 171

Query: 61  IEKQVGGSNDTQSVPALMNQTKQ-PITQTTVGKSSSHGSTSFGSKICCCLYPK 108
           IEKQ+GGSN+ Q+VP LMNQTK+ PI QTT   +++HGS SF SKICCCL PK
Sbjct: 172 IEKQIGGSNNPQAVPPLMNQTKKLPIAQTTT-TATTHGSNSFVSKICCCLCPK 223

[Close]

BLAST of Cucsat.G21544 vs. NCBI nr
Match: XP_038895247.1 (RPM1-interacting protein 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 156 bits (395), Expect = 2.57e-45
Identity = 80/106 (75.47%), Postives = 90/106 (84.91%), Query Frame = 0


Query: 1   MSRFSPATV----DGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK 60
           MSRF+P T      GKGNRNPRVRRN+EGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK
Sbjct: 112 MSRFTPTTTVSGHGGKGNRNPRVRRNDEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVK 171

Query: 61  IEKQVGGSNDTQSVPALMNQTKQ-PITQTTVGKSSSHGSTSFGSKI 101
           IEKQ+GGSN+ Q+VP LMNQTK+ PI QTT   +++HGS SF SK+
Sbjct: 172 IEKQIGGSNNPQAVPPLMNQTKKLPIAQTTT-TATTHGSNSFVSKV 216

[Close]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL
Match: A0A0A0M0M0 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G699040 PE=4 SV=1)

HSP 1 Score: 225 bits (574), Expect = 4.70e-73
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK 108
           VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK
Sbjct: 176 VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK 223

[Close]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL
Match: A0A1S3C9P5 (RPM1-interacting protein 4-like OS=Cucumis melo OX=3656 GN=LOC103498234 PE=4 SV=1)

HSP 1 Score: 198 bits (503), Expect = 2.92e-62
Identity = 94/107 (87.85%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSP TVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPTTVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYP 107
           +GGSN+ Q+VP LMNQTKQP+TQTTV K+S+ GSTSF SKICCCL P
Sbjct: 176 IGGSNNPQTVPPLMNQTKQPMTQTTVAKTSTQGSTSFVSKICCCLGP 222

[Close]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL
Match: A0A5A7UH42 (RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G001090 PE=4 SV=1)

HSP 1 Score: 185 bits (469), Expect = 6.52e-57
Identity = 88/101 (87.13%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           MSRFSP TVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ
Sbjct: 116 MSRFSPTTVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 175

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKI 101
           +GGSN+ Q+VP LMNQTKQP+TQTTV K+S+ GSTSF SK+
Sbjct: 176 IGGSNNPQTVPPLMNQTKQPMTQTTVAKTSTQGSTSFVSKV 216

[Close]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL
Match: A0A6J1CND0 (RPM1-interacting protein 4-like OS=Momordica charantia OX=3673 GN=LOC111012692 PE=4 SV=1)

HSP 1 Score: 144 bits (362), Expect = 6.74e-41
Identity = 74/110 (67.27%), Postives = 82/110 (74.55%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVR-RNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEK 60
           +SRF P      GN NP  R R NEGEM+ASVPKFGSWD RDPKSGDGYTAIFNKVK+EK
Sbjct: 119 ISRFPPTAAGHGGNPNPNPRARRNEGEMVASVPKFGSWDARDPKSGDGYTAIFNKVKMEK 178

Query: 61  QVGGSNDTQSVPALMNQTKQ-PITQTTVGKSSSHGSTSFGSKICCCLYPK 108
           ++GGSN +Q+VP L NQTKQ PI Q        HGSTSF SK+CCCL PK
Sbjct: 179 KIGGSNSSQAVPPLTNQTKQQPIPQIV------HGSTSFVSKVCCCLCPK 222

[Close]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL
Match: A0A6J1GSU2 (RPM1-interacting protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111457178 PE=4 SV=1)

HSP 1 Score: 114 bits (285), Expect = 1.48e-29
Identity = 63/108 (58.33%), Postives = 71/108 (65.74%), Query Frame = 0


Query: 1   MSRFSPATVDGKGNRNPRVRRNNEGEMMASVPKFGSWDVRDPKSGDGYTAIFNKVKIEKQ 60
           +SR +PA     GN              ASVPKFGSWD RDPKSGDGYTAIFNK+KIEKQ
Sbjct: 109 ISRLAPAVAGHGGN-------------PASVPKFGSWDARDPKSGDGYTAIFNKMKIEKQ 168

Query: 61  VGGSNDTQSVPALMNQTKQPITQTTVGKSSSHGSTSFGSKICCCLYPK 108
           +  SN  QSVP LMN+TKQPI  T    +++ G TSF SKICCCL  K
Sbjct: 169 IAASNP-QSVPPLMNKTKQPIAHT----NTTSGFTSFASKICCCLRTK 198

The following BLAST results are available for this feature:

BLAST of Cucsat.G21544 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYN5	1.7e-06	37.84	RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1	[more]
O22633	6.4e-06	60.61	Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1	[more]

BLAST of Cucsat.G21544 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011660296.1	9.70e-73	100.00	RPM1-interacting protein 4 [Cucumis sativus] >KGN66824.1 hypothetical protein Cs...	[more]
XP_008458983.1	6.04e-62	87.85	PREDICTED: RPM1-interacting protein 4-like [Cucumis melo]	[more]
KAA0054298.1	1.35e-56	87.13	RPM1-interacting protein 4-like [Cucumis melo var. makuwa] >TYK12335.1 RPM1-inte...	[more]
XP_038895249.1	1.27e-51	76.99	RPM1-interacting protein 4-like isoform X2 [Benincasa hispida]	[more]
XP_038895247.1	2.57e-45	75.47	RPM1-interacting protein 4-like isoform X1 [Benincasa hispida]	[more]

BLAST of Cucsat.G21544 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0M0	4.70e-73	100.00	AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G69...	[more]
A0A1S3C9P5	2.92e-62	87.85	RPM1-interacting protein 4-like OS=Cucumis melo OX=3656 GN=LOC103498234 PE=4 SV=...	[more]
A0A5A7UH42	6.52e-57	87.13	RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1CND0	6.74e-41	67.27	RPM1-interacting protein 4-like OS=Momordica charantia OX=3673 GN=LOC111012692 P...	[more]
A0A6J1GSU2	1.48e-29	58.33	RPM1-interacting protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111457178 PE...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008700	RIN4, pathogenic type III effector avirulence factor Avr cleavage site	PFAM	PF05627	AvrRpt-cleavage	coord: 144..175 e-value: 6.5E-15 score: 54.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..135
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 63..112
None	No IPR available	PANTHER	PTHR33159:SF49	OS06G0636100 PROTEIN	coord: 6..222
IPR040387	RPM1-interacting protein 4/NOI4	PANTHER	PTHR33159	RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEIN	coord: 6..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21544.T1	Cucsat.G21544.T1	mRNA
Cucsat.G21544.T3	Cucsat.G21544.T3	mRNA
Cucsat.G21544.T2	Cucsat.G21544.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005886	plasma membrane