Cucsat.G21542 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21542
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionvacuolar protein 8
Locationctg944: 729021 .. 731476 (+)
RNA-Seq ExpressionCucsat.G21542
SyntenyCucsat.G21542
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTTTGGAGTTTGGAGTGTGGAGGGAGAGGCACCGGCAAAGGATGCAAGTGGGGGTTTTAAGTATAAATAAGTTTTATTTATACTTTCTTATTTTTGGTGGTTTTCTTTCCTTCTCTCCAAAGTAGCTGCAAATTGTTGGCCTTTTCATACCTACGCACTTTCTTCACAAATCTCCCTCTTCTTCCTTCCTTTCTTCCTTCCTTTCTTCCTTCCTTCCTTCCTTCCTTCCTTCCCTCCTTCACTCTTCGCCATGAAAATACCTCCAGAAACCGACCATTTCCTTCTATCTAACAACCTCATTTCTTCTCTTCTTGACGATATTCCTCTCATCACCATTTTCAAAGGCAAGTGGTCTTCCATCAGAGCCAAACTCTCCGATCTTCGCACTCAACTCATCGATGTTTCCCACTTTCCAAACTCCTCTTCCAATCCCCTCTCTCTCGATTTCCTTCATTCTGTTCTCGAAGCTCTTACTCAGGCCGCTTCTCTCTCCCACAAGTGCCGGAATCCCGCTCTCTCCGATGGTAAACTCAAGACCCAGAGCGATATTGACGCTATTCTTGCTAAGTTTGACTCCCTTCTCAAAGACGGTGAGGTTTTGATCAGAAGTGAGATTCTTCACGACGGTGTTGTTTCCAGTTCTTCGTCTAGAAGAGAGGCTGTGAGAGCGGAGTCCAGGAATTTGATTACTAGGTTGCAGATTGGGAGTATTGAATCCAGGGTTTTGGCTATTGATTCGTTGTTGCAGTTGCTGAATGAGGATGATAAGAATGTTACTATCGCCGCAGCTCAAGGTGCTGTTCCTGTTCTTGTTCGGCTACTGGATTCCAGCTCTTTGGAATTGAAGGAAAGGGCTGTTGCTGCTATTTCTATTGTTTCTATGGTGGATGGCGTTAAGCACATCATGATTGCTGAAGGTCTAGTGCTTTTAAATCACTTGCTCAGGATTCTTGATTCCGGTAGCGGTTTTGCTAAGGAGAAGGCCTGTTTAGCCCTCCAACCTCTCAGTATTTCCAAGGAAAATGCTAGGTCTATCGGTTCTAGAGGAGGAATTTCATCTCTGTTGGAGATTTGTGAGGGCGGTACTCCTGGTTCTCAAGCTTCTGCAGCTGCGGTTTTGAGAAATCTTGCGTCATTTAGTGAAATCAAAGAGAATTTCATAGAAGAAAATGGGGTTATTGTTCTTTTGGGGCTTTTGGCCTCGGGAACTCCGTTGGCCCAAGAAAACGCAATTGGTTGTTTGTGTAATTTAGTTCTGGACGATGATAATCTGAAGCTCTTGATTGTTAGAGAAGGCGGAATCGAGTTCTTGAGAAATTTCTGGGATTCTGTTCCATCAGTTCGTAGTCTTGAGGTTGCTGTGGAGCTTTTAAGCCTCTTGGCTTCTTATTCCCCAATTGCAGAAGCTCTTATTTCAGATGGATTTGTCGATAGACTTCTTCCAGTTTTGAGTTGCGGAGTACTAGGTGCTAGAACTGCAGCAGCTCGAGCGGTTTATGAACTCGGATTCTGCACGAAAACGAGAAAAGAAATGGGGGAATCTGGATTCATTACACCCTTAGTTAATATGTTGGATGGTAAATCTGTTGATGAGAGAAAAGCAGCTGCTAAGGCATTGTCTTCTCTATTACAATACTCTGGTAATAGAAAAATTTTCCAGAAAGAAGAGAGGGGAATTGTAAGTGCAGTTCAACTCTTAGACCCTTCGATATCGAATCTCGACAAGAAGTACCCTGTTTCATTATTATCCTCAGTTGCGATTTCAAGCAAGTGTAGAAAGCAAATGGTTGCTGCTGGTGCTGGTTTGTATCTACAAAAGCTTGTTGAAATAAATGTTGAGGGGTCAAAGAAGCTATTGGAAAGCCTTGGCCGTGGTAAAATCTGGGGTGTCTTTGCCAGATCTTAGCCCATCAAGGAGGCAATTTTCCAAACTTCTGTTTATTGTATAGGAACAGATGCATGATTCGCTGTATGTACAATATATGTTTGTATATTCTTAGTTTTAATGGGTGTTGATTTTTAGTTTACAAGTTAATAAACTTTTTATCTGTTCTTATGTGGTAGAAATGTCAAAATGCTGCACCATGCCAGCTGAATTTGAGCATCACTTTACAGACACGATCTAGGTTTGATGACCCAACTTGATTTTGATTCTGTGATCATTACTGGAAACTTCATTGTCCAATAATCGAATGACACACCTTCAATTATTTTAGGAACTGTTCTCTATAATATTAGATTTTGCAGCTGTTATATTATTCTAATGAAGAAAATAAACTTCGATATCAGGATGAATTTTGTGTTCCATTCAACTGATGGAGAAAGGTTGGAAGGCCTTCTCTGAGTCTTCTTTGGTAATAAATTCTAGTCTGCATCTTTTATTCCATTATTAGGTCTTGGTAATCCTTGAACTTTAGAATACAACCCTTCTCTATTCTTCTATATATCTTTCAAA

Coding sequence (CDS)

ATGAAAATACCTCCAGAAACCGACCATTTCCTTCTATCTAACAACCTCATTTCTTCTCTTCTTGACGATATTCCTCTCATCACCATTTTCAAAGGCAAGTGGTCTTCCATCAGAGCCAAACTCTCCGATCTTCGCACTCAACTCATCGATGTTTCCCACTTTCCAAACTCCTCTTCCAATCCCCTCTCTCTCGATTTCCTTCATTCTGTTCTCGAAGCTCTTACTCAGGCCGCTTCTCTCTCCCACAAGTGCCGGAATCCCGCTCTCTCCGATGGTAAACTCAAGACCCAGAGCGATATTGACGCTATTCTTGCTAAGTTTGACTCCCTTCTCAAAGACGGTGAGGTTTTGATCAGAAGTGAGATTCTTCACGACGGTGTTGTTTCCAGTTCTTCGTCTAGAAGAGAGGCTGTGAGAGCGGAGTCCAGGAATTTGATTACTAGGTTGCAGATTGGGAGTATTGAATCCAGGGTTTTGGCTATTGATTCGTTGTTGCAGTTGCTGAATGAGGATGATAAGAATGTTACTATCGCCGCAGCTCAAGGTGCTGTTCCTGTTCTTGTTCGGCTACTGGATTCCAGCTCTTTGGAATTGAAGGAAAGGGCTGTTGCTGCTATTTCTATTGTTTCTATGGTGGATGGCGTTAAGCACATCATGATTGCTGAAGGTCTAGTGCTTTTAAATCACTTGCTCAGGATTCTTGATTCCGGTAGCGGTTTTGCTAAGGAGAAGGCCTGTTTAGCCCTCCAACCTCTCAGTATTTCCAAGGAAAATGCTAGGTCTATCGGTTCTAGAGGAGGAATTTCATCTCTGTTGGAGATTTGTGAGGGCGGTACTCCTGGTTCTCAAGCTTCTGCAGCTGCGGTTTTGAGAAATCTTGCGTCATTTAGTGAAATCAAAGAGAATTTCATAGAAGAAAATGGGGTTATTGTTCTTTTGGGGCTTTTGGCCTCGGGAACTCCGTTGGCCCAAGAAAACGCAATTGGTTGTTTGTGTAATTTAGTTCTGGACGATGATAATCTGAAGCTCTTGATTGTTAGAGAAGGCGGAATCGAGTTCTTGAGAAATTTCTGGGATTCTGTTCCATCAGTTCGTAGTCTTGAGGTTGCTGTGGAGCTTTTAAGCCTCTTGGCTTCTTATTCCCCAATTGCAGAAGCTCTTATTTCAGATGGATTTGTCGATAGACTTCTTCCAGTTTTGAGTTGCGGAGTACTAGGTGCTAGAACTGCAGCAGCTCGAGCGGTTTATGAACTCGGATTCTGCACGAAAACGAGAAAAGAAATGGGGGAATCTGGATTCATTACACCCTTAGTTAATATGTTGGATGGTAAATCTGTTGATGAGAGAAAAGCAGCTGCTAAGGCATTGTCTTCTCTATTACAATACTCTGGTAATAGAAAAATTTTCCAGAAAGAAGAGAGGGGAATTGTAAGTGCAGTTCAACTCTTAGACCCTTCGATATCGAATCTCGACAAGAAGTACCCTGTTTCATTATTATCCTCAGTTGCGATTTCAAGCAAGTGTAGAAAGCAAATGGTTGCTGCTGGTGCTGGTTTGTATCTACAAAAGCTTGTTGAAATAAATGTTGAGGGGTCAAAGAAGCTATTGGAAAGCCTTGGCCGTGGTAAAATCTGGGGTGTCTTTGCCAGATCTTAG

Protein sequence

MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSNPLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRSEILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAAQGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGFAKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIKENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSVPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGFCTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAVQLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLGRGKIWGVFARS
Homology
BLAST of Cucsat.G21542 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 94.4 bits (233), Expect = 4.4e-18
Identity = 101/390 (25.90%), Postives = 169/390 (43.33%), Query Frame = 0

Query: 28  TIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSNPLSLDFLHSVLEALTQAASLSHKCRN- 87
           ++  G+ S      S   T  +    FP + +N  S +  H    A   ++  S + R+ 
Sbjct: 427 SVENGQTSENHHHRSPSATSTVSNEEFPRADANENSEESAH----ATPYSSDASGEIRSG 486

Query: 88  PALSDGKLKTQSDIDAILAKFDSLLKDGEVLIR-SEILHDGVVS--SSSSRRE--AVRAE 147
           P  +     T+ D+     KF      G+   R SE L   +VS  S+ +RR+   V  +
Sbjct: 487 PLAATTSAATRRDLSDFSPKFMDRRTRGQFWRRPSERLGSRIVSAPSNETRRDLSEVETQ 546

Query: 148 SRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAAQGAVPVLVRLLDSSSLELKER 207
            + L+  L+  S++++  A   L  L   +  N  +    GA+ +LV LL S+    +E 
Sbjct: 547 VKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQEN 606

Query: 208 AVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGFAKEKACLALQPLSISKENARS 267
           AV A+  +S+ D  K  +   G +    L+ +L++GS  AKE +   L  LS+ +EN   
Sbjct: 607 AVTALLNLSINDNNKKAIADAGAI--EPLIHVLENGSSEAKENSAATLFSLSVIEENKIK 666

Query: 268 IGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIKENFIEENGVIVLLGLLASGTP 327
           IG  G I  L+++   GTP  +  AA  L NL+   E K   ++   V  L+ L+     
Sbjct: 667 IGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDLMDPAAG 726

Query: 328 LAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSVPSVRSLEVAVELLSLLASYS 387
           +  + A+  L NL    +  +  I +EGGI  L    +   +      A  LL L  +  
Sbjct: 727 MV-DKAVAVLANLATIPEG-RNAIGQEGGIPLLVEVVELGSARGKENAAAALLQLSTNSG 786

Query: 388 PIAEALISDGFVDRLLPVLSCGVLGARTAA 412
                ++ +G V  L+ +   G   AR  A
Sbjct: 787 RFCNMVLQEGAVPPLVALSQSGTPRAREKA 808

BLAST of Cucsat.G21542 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 1.2e-15
Identity = 71/273 (26.01%), Postives = 126/273 (46.15%), Query Frame = 0

Query: 143 RNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAAQGAVPVLVRLLDSSSLELKERA 202
           R L+ RL   S E R  A+  +  L      N  + A  GA+PVLV LL S  +  +E A
Sbjct: 334 RALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENA 393

Query: 203 VAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGFAKEKACLALQPLSISKENARSI 262
           +  +  +S+ +  K +++  G V    ++++L +G+  A+E A   L  LS++ EN   I
Sbjct: 394 ITCVLNLSIYENNKELIMFAGAV--TSIVQVLRAGTMEARENAAATLFSLSLADENKIII 453

Query: 263 GSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIKENFIEENGVIVLLGLLASGTPL 322
           G  G I +L+++ E GTP  +  AA  L NL  +   K   +    V  L+ +L+  T  
Sbjct: 454 GGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRH 513

Query: 323 AQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSVPSVRSLEVAVELLSLLASYSP 382
              +    + +++ ++ + K  IV+   +  L     +  +      A  LLSL    + 
Sbjct: 514 RMVDEALTILSVLANNQDAKSAIVKANTLPALIGILQTDQTRNRENAAAILLSLCKRDT- 573

Query: 383 IAEALISDGFVDRLLPVLSCGVLGARTAAARAV 416
             E LI+ G +  ++P++     G      +A+
Sbjct: 574 --EKLITIGRLGAVVPLMDLSKNGTERGKRKAI 601

BLAST of Cucsat.G21542 vs. ExPASy Swiss-Prot
Match: Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.1e-13
Identity = 82/306 (26.80%), Postives = 137/306 (44.77%), Query Frame = 0

Query: 125 DGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAAQGAV 184
           DG     S    A+RA    L+ +L   SIE R  A+  +  L      N  + A  GA+
Sbjct: 330 DGSFRDLSGDMSAIRA----LVCKLSSQSIEDRRTAVSEIRSLSKRSTDNRILIAEAGAI 389

Query: 185 PVLVRLLDS-SSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGFAKE 244
           PVLV+LL S    E +E AV  I  +S+ +  K +++  G V    ++ +L +GS  A+E
Sbjct: 390 PVLVKLLTSDGDTETQENAVTCILNLSIYEHNKELIMLAGAV--TSIVLVLRAGSMEARE 449

Query: 245 KACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIKENF 304
            A   L  LS++ EN   IG+ G I +L+++ + G+   +  AA  L NL  +   K   
Sbjct: 450 NAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATALFNLCIYQGNKGRA 509

Query: 305 IEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSVPS 364
           +    V  L+ +L   +     +    + +++  +   K  I+R   I  L +       
Sbjct: 510 VRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQVAKTAILRANAIPPLIDCLQK-DQ 569

Query: 365 VRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGFCTK 424
            R+ E A  +L  L       E LIS G +  ++P++     G   A  +A   L    K
Sbjct: 570 PRNRENAAAILLCLCKRD--TEKLISIGRLGAVVPLMELSRDGTERAKRKANSLLELLRK 626

Query: 425 TRKEMG 430
           + +++G
Sbjct: 630 SSRKLG 626

BLAST of Cucsat.G21542 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 5.6e-13
Identity = 70/238 (29.41%), Postives = 119/238 (50.00%), Query Frame = 0

Query: 184 VPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAE--GLVLLNHLLRILDSGSGFA 243
           V +LV  L SS LE + R+V  + +++  +    ++IA    + LL  LL   DSG    
Sbjct: 381 VSLLVEALSSSQLEEQRRSVKQMRLLARENPENRVLIANAGAIPLLVQLLSYPDSG---I 440

Query: 244 KEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIKE 303
           +E A   L  LSI + N + I + G I +++EI E G   ++ ++AA L +L+   E K 
Sbjct: 441 QENAVTTLLNLSIDEVNKKLISNEGAIPNIIEILENGNREARENSAAALFSLSMLDENKV 500

Query: 304 NFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSV 363
                NG+  L+ LL  GT   +++A+  L NL L+  N K   +  G ++ L N     
Sbjct: 501 TIGLSNGIPPLVDLLQHGTLRGKKDALTALFNLSLNSAN-KGRAIDAGIVQPLLNLLKD- 560

Query: 364 PSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELG 420
            ++  ++ A+ +L LLAS+    +A+    F++ L+  +  G    +  A   + ELG
Sbjct: 561 KNLGMIDEALSILLLLASHPEGRQAIGQLSFIETLVEFIRQGTPKNKECATSVLLELG 613

BLAST of Cucsat.G21542 vs. ExPASy Swiss-Prot
Match: Q2U5T5 (Vacuolar protein 8 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=vac8 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 6.8e-11
Identity = 74/334 (22.16%), Postives = 148/334 (44.31%), Query Frame = 0

Query: 145 LITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAAQGAVPVLVRLLDSSSLELKERAVA 204
           LI ++   ++E +  A+  +  L   +D    IA + GA+  L+RL  S  + ++  A  
Sbjct: 152 LIRQMMSPNVEVQCNAVGCITNLATHEDNKAKIARS-GALGPLIRLAKSKDMRVQRNATG 211

Query: 205 AISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGFAKEKACLALQPLSISKENARSIGS 264
           A+  ++  D  +  ++  G + +  L+++L S     +     AL  +++   N + +  
Sbjct: 212 ALLNMTHSDDNRQQLVNAGAIPV--LVQLLSSSDVDVQYYCTTALSNIAVDASNRKRLAQ 271

Query: 265 RGG--ISSLLEICEGGTPGSQASAAAVLRNLASFSEIKENFIEENGVIVLLGLLASGTPL 324
                + SL+ + +  TP  Q  AA  LRNLAS  + +   +   G+  LL LL S    
Sbjct: 272 TESRLVQSLVHLMDSSTPKVQCQAALALRNLASDEKYQLEIVRAKGLPPLLRLLQSSYLP 331

Query: 325 AQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDSVPSVRSLEVAVELLS----LLA 384
              +A+ C+ N+ +   N   +I  + G  FL+   D + S  + E+    +S    L A
Sbjct: 332 LILSAVACIRNISIHPLNESPII--DAG--FLKPLVDLLGSTDNEEIQCHAISTLRNLAA 391

Query: 385 SYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGFCTKTRKEMGESGFITPLV 444
           S     E ++  G V +   ++    L  ++    A+  L    + +  +   G    L+
Sbjct: 392 SSDRNKELVLQAGAVQKCKDLVLKVPLSVQSEMTAAIAVLALSDELKPHLLNLGVFDVLI 451

Query: 445 NMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKE 473
            + + +S++ +  +A AL +L    G+  IF ++
Sbjct: 452 PLTESESIEVQGNSAAALGNLSSKVGDYSIFVRD 478

BLAST of Cucsat.G21542 vs. NCBI nr
Match: XP_004145464.1 (vacuolar protein 8 [Cucumis sativus] >KGN66829.1 hypothetical protein Csa_006888 [Cucumis sativus])

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS
Sbjct: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. NCBI nr
Match: XP_008458985.1 (PREDICTED: vacuolar protein 8 [Cucumis melo] >KAA0054293.1 vacuolar protein 8 [Cucumis melo var. makuwa] >TYK12330.1 vacuolar protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 543/551 (98.55%), Postives = 549/551 (99.64%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPP TDHFLLSNNL+SSLLDDIPLI+IFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPHTDHFLLSNNLLSSLLDDIPLISIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDA+LAKFDSLLKDGEVLIRS
Sbjct: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDALLAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGV+VLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 ENFIEENGVMVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPS RSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSARSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGI+SAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIISAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVE+NVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEMNVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. NCBI nr
Match: XP_038895178.1 (vacuolar protein 8 [Benincasa hispida] >XP_038895179.1 vacuolar protein 8 [Benincasa hispida] >XP_038895180.1 vacuolar protein 8 [Benincasa hispida])

HSP 1 Score: 1008 bits (2605), Expect = 0.0
Identity = 536/551 (97.28%), Postives = 546/551 (99.09%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPPETDHFLLSNNLISSLLDDIPLI  FKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPETDHFLLSNNLISSLLDDIPLINNFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           P+SLDFLHSVL+ALTQAASLSHKCRNPALSDGKLKTQSDIDA+LAKFDSLLKDGEVLIRS
Sbjct: 61  PISLDFLHSVLDALTQAASLSHKCRNPALSDGKLKTQSDIDAVLAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKE+ACLALQPLSISKENARSIGSRGGISSLLEICE GTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKERACLALQPLSISKENARSIGSRGGISSLLEICEAGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
            NFIEENGV+VLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 GNFIEENGVVVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDE+KAAAKALSSLLQYSGNR+IFQKEERGIVSAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDEKKAAAKALSSLLQYSGNRRIFQKEERGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSS+ ISSKCRKQMVAAGAGLYL+KLVE+NVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSLVISSKCRKQMVAAGAGLYLRKLVEMNVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. NCBI nr
Match: XP_022137698.1 (vacuolar protein 8 isoform X2 [Momordica charantia] >XP_022137699.1 vacuolar protein 8 isoform X2 [Momordica charantia])

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 493/551 (89.47%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MK+P E DHF +SN LISSLLDDIP+I+ FKGKWSSIRAKLSDLRTQL+DVS FPNSSSN
Sbjct: 1   MKVPLENDHFAVSNQLISSLLDDIPVISNFKGKWSSIRAKLSDLRTQLLDVSEFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSV EAL++A SLS +CRNP LS GKLKTQSD+D++LAK D LL+DG+VLIRS
Sbjct: 61  PLSLDFLHSVFEALSEATSLSQRCRNPELSGGKLKTQSDVDSVLAKLDCLLRDGDVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDG VSSSSS+REAVRAESRNLITRLQIGSIESR+ AIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGAVSSSSSKREAVRAESRNLITRLQIGSIESRIFAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QG VPVLVRLLDSSSLELKERAVAAISIVSMVDGVKH++IAEGL LLNHLLRILDSGSGF
Sbjct: 181 QGVVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHVLIAEGLPLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACL LQPLSISKENARSIGSRGGI+SLLEICE GTPGSQASAAAVLRNLA FSEIK
Sbjct: 241 AKEKACLTLQPLSISKENARSIGSRGGIASLLEICEAGTPGSQASAAAVLRNLALFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
            NF+EENGVIVLLGLLASGTPLAQENAIGCLCNLV+DDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 GNFMEENGVIVLLGLLASGTPLAQENAIGCLCNLVVDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
            PSVRSLEVAVELL LLA YSP+AEALISDGF+DRLLPVLSCGVLG RTAAARAVYELGF
Sbjct: 361 APSVRSLEVAVELLRLLALYSPLAEALISDGFIDRLLPVLSCGVLGVRTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGE+GFITPLVNMLDGKSVDE++AAAKALSSLLQY+GNRKIFQKEERGIVSAV
Sbjct: 421 CTKTRKEMGEAGFITPLVNMLDGKSVDEKEAAAKALSSLLQYTGNRKIFQKEERGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSI+NLDKKYPVSLLSSV ISSKCRKQM AA AGLYLQKLVE+NVEGSKKLLESL 
Sbjct: 481 QLLDPSITNLDKKYPVSLLSSVVISSKCRKQMAAADAGLYLQKLVEMNVEGSKKLLESLV 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVF RS
Sbjct: 541 RGKIWGVFPRS 551

BLAST of Cucsat.G21542 vs. NCBI nr
Match: XP_023542631.1 (vacuolar protein 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 497/551 (90.20%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           M IPPE D F LSN+ ISSLLDDIPLI  FKGKWSSIRAKLSDLRTQLIDVS FPNSSSN
Sbjct: 1   MTIPPENDQFALSNDRISSLLDDIPLINNFKGKWSSIRAKLSDLRTQLIDVSEFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLS+DFLHSVLE L++AASLS KCRNP LSDGKLKTQSDID++LAK DSLLKDGEVLIRS
Sbjct: 61  PLSVDFLHSVLEVLSEAASLSEKCRNPELSDGKLKTQSDIDSVLAKLDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDG VSSSSSRREAVRAESRNLITRLQIGSIESRVLAI+SLL+LLNEDDKNVTIAAA
Sbjct: 121 EILHDGAVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIESLLKLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QG VPVLVRLLDSSSLELKERAVAAISIVS VDGV+H+MIAEGL LLNHLLRILDSGSGF
Sbjct: 181 QGIVPVLVRLLDSSSLELKERAVAAISIVSSVDGVRHLMIAEGLQLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACL LQPLSISKENARSIGSRGGISSLLEICE GTPGSQASAAAVLRNLASF EIK
Sbjct: 241 AKEKACLTLQPLSISKENARSIGSRGGISSLLEICEAGTPGSQASAAAVLRNLASFGEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLV +DDNLKLLIVREGGIE LR+FWDS
Sbjct: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVAEDDNLKLLIVREGGIELLRSFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
            PSV SLEVAVELL LLAS +PIAEALIS+GFVDRLLP LSCGVLG RTAAARAVYELGF
Sbjct: 361 APSVHSLEVAVELLGLLASSAPIAEALISNGFVDRLLPALSCGVLGVRTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRK+MGE+GFITPLVNMLDGKSV+E++AAAKALSSLLQY+GNRKIFQKEE+GIVSAV
Sbjct: 421 CTKTRKDMGEAGFITPLVNMLDGKSVEEKEAAAKALSSLLQYTGNRKIFQKEEKGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLL+SV ISSKCRKQM AAGAGLYLQKLVE+NV+GSKKLLE LG
Sbjct: 481 QLLDPSISNLDKKYPVSLLASVMISSKCRKQMAAAGAGLYLQKLVEMNVDGSKKLLECLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. ExPASy TrEMBL
Match: A0A0A0M0M7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G699580 PE=4 SV=1)

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS
Sbjct: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. ExPASy TrEMBL
Match: A0A5A7UE60 (Vacuolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G001020 PE=4 SV=1)

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 543/551 (98.55%), Postives = 549/551 (99.64%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPP TDHFLLSNNL+SSLLDDIPLI+IFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPHTDHFLLSNNLLSSLLDDIPLISIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDA+LAKFDSLLKDGEVLIRS
Sbjct: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDALLAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGV+VLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 ENFIEENGVMVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPS RSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSARSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGI+SAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIISAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVE+NVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEMNVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. ExPASy TrEMBL
Match: A0A1S3C8N4 (vacuolar protein 8 OS=Cucumis melo OX=3656 GN=LOC103498236 PE=4 SV=1)

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 543/551 (98.55%), Postives = 549/551 (99.64%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPP TDHFLLSNNL+SSLLDDIPLI+IFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN
Sbjct: 1   MKIPPHTDHFLLSNNLLSSLLDDIPLISIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDA+LAKFDSLLKDGEVLIRS
Sbjct: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDALLAKFDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF
Sbjct: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK
Sbjct: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGV+VLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 ENFIEENGVMVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
           VPS RSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF
Sbjct: 361 VPSARSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGI+SAV
Sbjct: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIISAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVE+NVEGSKKLLESLG
Sbjct: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEMNVEGSKKLLESLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

BLAST of Cucsat.G21542 vs. ExPASy TrEMBL
Match: A0A6J1CB33 (vacuolar protein 8 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009071 PE=4 SV=1)

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 493/551 (89.47%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MK+P E DHF +SN LISSLLDDIP+I+ FKGKWSSIRAKLSDLRTQL+DVS FPNSSSN
Sbjct: 1   MKVPLENDHFAVSNQLISSLLDDIPVISNFKGKWSSIRAKLSDLRTQLLDVSEFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLSLDFLHSV EAL++A SLS +CRNP LS GKLKTQSD+D++LAK D LL+DG+VLIRS
Sbjct: 61  PLSLDFLHSVFEALSEATSLSQRCRNPELSGGKLKTQSDVDSVLAKLDCLLRDGDVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDG VSSSSS+REAVRAESRNLITRLQIGSIESR+ AIDSLLQLLNEDDKNVTIAAA
Sbjct: 121 EILHDGAVSSSSSKREAVRAESRNLITRLQIGSIESRIFAIDSLLQLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QG VPVLVRLLDSSSLELKERAVAAISIVSMVDGVKH++IAEGL LLNHLLRILDSGSGF
Sbjct: 181 QGVVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHVLIAEGLPLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACL LQPLSISKENARSIGSRGGI+SLLEICE GTPGSQASAAAVLRNLA FSEIK
Sbjct: 241 AKEKACLTLQPLSISKENARSIGSRGGIASLLEICEAGTPGSQASAAAVLRNLALFSEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
            NF+EENGVIVLLGLLASGTPLAQENAIGCLCNLV+DDDNLKLLIVREGGIEFLRNFWDS
Sbjct: 301 GNFMEENGVIVLLGLLASGTPLAQENAIGCLCNLVVDDDNLKLLIVREGGIEFLRNFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
            PSVRSLEVAVELL LLA YSP+AEALISDGF+DRLLPVLSCGVLG RTAAARAVYELGF
Sbjct: 361 APSVRSLEVAVELLRLLALYSPLAEALISDGFIDRLLPVLSCGVLGVRTAAARAVYELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGE+GFITPLVNMLDGKSVDE++AAAKALSSLLQY+GNRKIFQKEERGIVSAV
Sbjct: 421 CTKTRKEMGEAGFITPLVNMLDGKSVDEKEAAAKALSSLLQYTGNRKIFQKEERGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSI+NLDKKYPVSLLSSV ISSKCRKQM AA AGLYLQKLVE+NVEGSKKLLESL 
Sbjct: 481 QLLDPSITNLDKKYPVSLLSSVVISSKCRKQMAAADAGLYLQKLVEMNVEGSKKLLESLV 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVF RS
Sbjct: 541 RGKIWGVFPRS 551

BLAST of Cucsat.G21542 vs. ExPASy TrEMBL
Match: A0A6J1K4Z6 (vacuolar protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111490111 PE=4 SV=1)

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 498/551 (90.38%), Postives = 522/551 (94.74%), Query Frame = 0

Query: 1   MKIPPETDHFLLSNNLISSLLDDIPLITIFKGKWSSIRAKLSDLRTQLIDVSHFPNSSSN 60
           MKIPPE D F LSN+ ISSLLDDIPLI  FKGKWSSIRAKLSDLRTQLIDVS FPNSSSN
Sbjct: 1   MKIPPENDQFSLSNDRISSLLDDIPLINNFKGKWSSIRAKLSDLRTQLIDVSEFPNSSSN 60

Query: 61  PLSLDFLHSVLEALTQAASLSHKCRNPALSDGKLKTQSDIDAILAKFDSLLKDGEVLIRS 120
           PLS+DFLHSVL+AL++AA LS KCRNP LSDGKLKTQSDID++LAK DSLLKDGEVLIRS
Sbjct: 61  PLSVDFLHSVLKALSEAAFLSEKCRNPELSDGKLKTQSDIDSVLAKLDSLLKDGEVLIRS 120

Query: 121 EILHDGVVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIDSLLQLLNEDDKNVTIAAA 180
           EILHDG VSSSSSRREAVRAESRNLITRLQIGSIESRVLAI+SLL+LLNEDDKNVTIAAA
Sbjct: 121 EILHDGAVSSSSSRREAVRAESRNLITRLQIGSIESRVLAIESLLKLLNEDDKNVTIAAA 180

Query: 181 QGAVPVLVRLLDSSSLELKERAVAAISIVSMVDGVKHIMIAEGLVLLNHLLRILDSGSGF 240
           QG VPVLVRLLDSSSLELKERAVAAISIVS VDGV+H+MIAEGL LLNHLLRILDSGSGF
Sbjct: 181 QGIVPVLVRLLDSSSLELKERAVAAISIVSSVDGVRHLMIAEGLQLLNHLLRILDSGSGF 240

Query: 241 AKEKACLALQPLSISKENARSIGSRGGISSLLEICEGGTPGSQASAAAVLRNLASFSEIK 300
           AKEKACL LQPLSISKENARSIGSRGGISSLLEICE GTPGSQASAAAVLRNLASF EIK
Sbjct: 241 AKEKACLTLQPLSISKENARSIGSRGGISSLLEICEAGTPGSQASAAAVLRNLASFGEIK 300

Query: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVLDDDNLKLLIVREGGIEFLRNFWDS 360
           ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLV +DDNLKLLIVREGGIE LR+FWDS
Sbjct: 301 ENFIEENGVIVLLGLLASGTPLAQENAIGCLCNLVAEDDNLKLLIVREGGIELLRSFWDS 360

Query: 361 VPSVRSLEVAVELLSLLASYSPIAEALISDGFVDRLLPVLSCGVLGARTAAARAVYELGF 420
            PSV SLEVAVELL LLAS SPIAEALIS+GFVDRLLP LSCGVLG RTAAARAV+ELGF
Sbjct: 361 APSVHSLEVAVELLGLLASSSPIAEALISNGFVDRLLPALSCGVLGVRTAAARAVHELGF 420

Query: 421 CTKTRKEMGESGFITPLVNMLDGKSVDERKAAAKALSSLLQYSGNRKIFQKEERGIVSAV 480
           CTKTRKEMGE+GFITPLVNMLDGKSV+E++AAAKALSSLLQY+GNRKIFQKEE+GIVSAV
Sbjct: 421 CTKTRKEMGEAGFITPLVNMLDGKSVEEKEAAAKALSSLLQYTGNRKIFQKEEKGIVSAV 480

Query: 481 QLLDPSISNLDKKYPVSLLSSVAISSKCRKQMVAAGAGLYLQKLVEINVEGSKKLLESLG 540
           QLLDPSISNLDKKYPVSLL+SV ISSKCRKQM AAGAGLYLQKLVE+NV+GSKKLLE LG
Sbjct: 481 QLLDPSISNLDKKYPVSLLASVMISSKCRKQMAAAGAGLYLQKLVEMNVDGSKKLLECLG 540

Query: 541 RGKIWGVFARS 551
           RGKIWGVFARS
Sbjct: 541 RGKIWGVFARS 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O221934.4e-1825.90U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q8GUG91.2e-1526.01U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q9C9A61.1e-1326.80U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2... [more]
Q681N25.6e-1329.41U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
Q2U5T56.8e-1122.16Vacuolar protein 8 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 ... [more]
Match NameE-valueIdentityDescription
XP_004145464.10.0100.00vacuolar protein 8 [Cucumis sativus] >KGN66829.1 hypothetical protein Csa_006888... [more]
XP_008458985.10.098.55PREDICTED: vacuolar protein 8 [Cucumis melo] >KAA0054293.1 vacuolar protein 8 [C... [more]
XP_038895178.10.097.28vacuolar protein 8 [Benincasa hispida] >XP_038895179.1 vacuolar protein 8 [Benin... [more]
XP_022137698.10.089.47vacuolar protein 8 isoform X2 [Momordica charantia] >XP_022137699.1 vacuolar pro... [more]
XP_023542631.10.090.20vacuolar protein 8 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0M0M70.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G699580 PE=4 SV=1[more]
A0A5A7UE600.098.55Vacuolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G0... [more]
A0A1S3C8N40.098.55vacuolar protein 8 OS=Cucumis melo OX=3656 GN=LOC103498236 PE=4 SV=1[more]
A0A6J1CB330.089.47vacuolar protein 8 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009071 PE=... [more]
A0A6J1K4Z60.090.38vacuolar protein 8 OS=Cucurbita maxima OX=3661 GN=LOC111490111 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 221..261
e-value: 0.1
score: 21.7
coord: 430..470
e-value: 66.0
score: 6.9
coord: 387..429
e-value: 12.0
score: 12.8
coord: 346..386
e-value: 1.8
score: 17.6
coord: 305..345
e-value: 20.0
score: 10.9
coord: 471..511
e-value: 1.9
score: 17.5
IPR000225ArmadilloPFAMPF00514Armcoord: 227..257
e-value: 6.8E-5
score: 22.8
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 232..260
score: 8.9774
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 181..407
e-value: 1.6E-31
score: 111.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 408..590
e-value: 4.4E-17
score: 63.7
NoneNo IPR availablePANTHERPTHR46043:SF14ARMADILLO-TYPE FOLD PROTEIN-RELATEDcoord: 58..599
NoneNo IPR availablePANTHERPTHR46043ARM REPEAT SUPERFAMILY PROTEINcoord: 58..599
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 194..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21542.T1Cucsat.G21542.T1mRNA
Cucsat.G21542.T2Cucsat.G21542.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding