Cucsat.G21316 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G21316
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	GDSL esterase/lipase
Location	ctg910: 933806 .. 935194 (+)
RNA-Seq Expression	Cucsat.G21316
Synteny	Cucsat.G21316

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCAGATTCCAAAATGACCATTTCAAGTCGTAGTTGTTTGCTTACATGCACATATTATGTCTCTTCTAGCATGACACTATCAACTTTTTTAGCACAGATGGTTAAATAATCCAACAGCTACGAGTCGAGCCTTGGGAATGTTTGAAAAAAAAATGGAGTTTTCTAAATCATATGTTGTGATGGTCATTGTTTTCTTCATCAACTTCTTTCATGGTGGTTTTTGTTTCACGACCTTTGTGTTTGGAGACTCGCTTGTTGATGCAGGGAATAACGACTATATTTTCACCCTATCCAAAGCTGATTCTCCACCATACGGTATTGATTTTAAACCCTCCGGTGGCCAACCCACAGGAAGGTTCACCAACGGTCGAACTATTTCAGATATTATCGGTAATGCATCCCTCCAAACTATACAGTATATATATCAAACAGTTATCACCTTGATCTATAACTATATATAAGTCAAAGGTATATAAGAATCGATCTCAAGTACGCCTCATATATTATTAAACTAACAACGTTGTTATTGACAACAAGTTATACATACATATGTATATACAATCTTAGCGATCAGTTTTCTATCAAAAGTAATAAAAGTAATTAAGCATGTGATGTAGGATTGTTTATATTATGGAATGTTATTTGAATCAGGGGAATATCTTGGAGCCAAATCATTTCCTCCACCATTTCTAGCTCCAATAAGTACACAATCAGACACAATAATATATAAAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTCTTTTATTTGTAAGTAATTAATGACTCAATATGTTGTTTTCTTCTTTCTAATAACACCATTTTTAATTTGTCCCAAAACGCTTTGAATTTAGTTCAATTTTAACATCTTTACTTTCAAGTGTTCCTTATATCAAATTTTCGTTCAATTTTAATATCTCTACTTTCAAATGTTCCCTATATCATATTAAGTTAACTTTTGAGGGACTAAATTTAAGATTGATTTAAAGTATGGACTAAAATTGAAAAAAAAAATCAAAGTACAAGGTTTGAAATAGTATATTTTAACTTTCCTTTTCTTCTAATATATATATTATGAACAGTTGGGAAGGATTTCACTTAGGGAACAAGTGAAGAATTTTGAGGAGAGTAGAAATGCGATGGTGAAAGTGAAGGGAGAGAATGAAACAATGGAGGTTTTGAAGAATTCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAATTATATTCAACCCTCCATTCCATTTCTCCAAACAAACAAGCCTTCCCCAAGTGATTACTTGGATCATATGATCTCAAATTTAACAGTACACCTCAAGGTAAGATAAATTGATTAAGAAAAATCTATTAGTTTCTTTTCATTGAGTTTATATATCTTTAATTAATTCCACCGTTTTATATCTACAACATAGTACTTGAAAAGAAAATGGTTTATATGTAACCCTTTCTAGCTATATAACAACTCTTAGTTCCTATAGCTCTTGTCTTGGTTTCAATCAAGACCTTAAGTTATATAAATCTCCTTTAATATTCTTATAGTTTTTAAAAAAGTTAATAGTTTTTCATGCATCAATTATTCTCTCTTTTGGTAGGGTAGAGAGAGATGATGAGCTAATATTATTCAAAAGAAAATGTATAAAAATAAGAAAAACAAATAAAAGAATTTATTATTTGGGGAATTAGCAAAACTATAGCAAACCATGAAATCAATTTAAAAACGATAAACAATTTCCTTTTAACAAAATCCAAAATGAAAGCATCGGAGAACCATAAAGATAAAAGATGAAACATAAATTTTCTTCGAACCCATGAAAATTAGATTACTCTTTCTAATTGGATATTGGAATCATTCTATTGATCAATGCAAGAGATTTTGGTAACTAATTATGTTTTTGAAAAAAATAAAAAGGAAAAAACAACTTTGATCGATCTTCTCTACATTTTCAAAATTCTTTTTGCTATTGTGACTTAAACATTTAATCATGGATAATGACAGACAATGAACTAAATATGAATTCTCTTATTTTTGTATGAATTACTATAAAAAACGTGTTTTGTTTTGATATCTCACCTCCTAAAGCCAAGGACACACCCTTCACTGAAGGGACAAAAAGGTGATCCCACCATACATGATGGACGATGCCAATCTTTTGTATGGTGAGACACTTTAGTGTTGGAACCAATTTTAGGACAATTATATCTTGGCAAGACTTGAAGAATAAGAGTATGACTTGGGGGCAAAGAAAAACTTAACAAAGCTAGTAATTACTTAAAGCTTACTTTTCCTACTTTTGATCAAAACACAAATCACCCTAATGGGTCATTTTTTAATACAATTAAAAAGTATCCGATACTTACTAGTTTATATTGAATATGGTTATTTAATTTGTATATTTCTATATTTGGATAGTAAATTTATGATTTGGAAAATATAGTTGGATAATAAATGAAGTTGTTTCATACGTCTAAATTTATAATTAGGTAGCGTGATAATAATTATATGTGTTTACTTATGAAGCGATTGCATGCGTTGGGAGCACGAAAGTTCGTAGTGGTTGGAGTTGGACCGCTTGGTTGCATTCCATTCGTACGAGCGATACATTTTGTAACAAATGAAAAATGCTTAGAAGAAGTGAATCAATTGATCGAAACCTATAATTTTCGGCTTAATGGAGCGGTTGATCAATTAAACCTAGAGTTTGGTCTTAGTACCATGTTCATTTATGCCAATTCATATGCGGTCTTCACGAAGATTATTGTCAATTATCGACAATATGGTTAGACTCCAAATCTCATCTTCTTATAATTCGATGTTATTCTATTTCAAAGTTCGATTTAAGTTTTTCTTTTAAAGAAAGATTGTTTCATCATTTTAGTCTAGATAGTTTGAGCATTTAATTTTATTTTAGCTCCTATATATATTTTCTTAAAAAAATTCAAACAGTTAACTTTTACATTTAATCCATGACATGAATTATCAGTCATGCTTCATTGTAGTAGAGAAAAGTAAGATACATTGACCTTTAGGTATGGGTTAAAAGGAAAAATTGAATGACCATATAGGACTTTGTTGTGGTTTAGAGTTTGAACTTTAGTCCAAATAAAGAAAATTTGGTGGGATTTAGGTAAGAAGGAAATAGGTTGTGTAGAAAAAAGGGGTGTCTTTGAATACCATACAATATTACAAAAGTTCTAATCCCAACACACCTATAATTTTCTCTTTTTCCATGTAAAAAAATAATAATAAACAAAGAAGTGGGTATTAACGTAAACATTATATACTAGATGCATCATTAATAAATCACGTAAATATATGTATCCTTCTTTTTCCGGGTGAAATATTTTAATGGCTTTTCAAACATGAAAATAGGGTTTGTGAATGCAAAGCAACCATGTTGTGTTGGATACTTTCCTCCTTTCATTTGCTACAAGGATCAAAACCAATCATCAAGCTCTTTCTTGTGTGAGGATCGATCTAAATATGTGTTTTGGGATGCATATCACCCAACAGAAGCTGCTAATATCATAATTGCCAAAGAGCTCCTCGATGGAGATGAAACCATTACTTCTCCCATCAACATTCGTCAATTGTATGCCTATCGTTTATAATGTACTGTGTTTGATTTTAGCTATGTTTGTATTGGTTATTTTTACATGTGATGTGTAAGTTCTTCTCATATATAAAATAGATGTGTTTTTCTTATCGTGGCCTATTTAAATATGGATAACCACACAATGGATATATAAAAATAAAAAAAAA

Coding sequence (CDS)

ATGTTTGAAAAAAAAATGGAGTTTTCTAAATCATATGTTGTGATGGTCATTGTTTTCTTCATCAACTTCTTTCATGGTGGTTTTTGTTTCACGACCTTTGTGTTTGGAGACTCGCTTGTTGATGCAGGGAATAACGACTATATTTTCACCCTATCCAAAGCTGATTCTCCACCATACGGTATTGATTTTAAACCCTCCGGTGGCCAACCCACAGGAAGGTTCACCAACGGTCGAACTATTTCAGATATTATCGGGGAATATCTTGGAGCCAAATCATTTCCTCCACCATTTCTAGCTCCAATAAGTACACAATCAGACACAATAATATATAAAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTCTTTTATTTTTGGGAAGGATTTCACTTAGGGAACAAGTGAAGAATTTTGAGGAGAGTAGAAATGCGATGGTGAAAGTGAAGGGAGAGAATGAAACAATGGAGGTTTTGAAGAATTCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAATTATATTCAACCCTCCATTCCATTTCTCCAAACAAACAAGCCTTCCCCAAGTGATTACTTGGATCATATGATCTCAAATTTAACAGTACACCTCAAGCGATTGCATGCGTTGGGAGCACGAAAGTTCGTAGTGGTTGGAGTTGGACCGCTTGGTTGCATTCCATTCGTACGAGCGATACATTTTGTAACAAATGAAAAATGCTTAGAAGAAGTGAATCAATTGATCGAAACCTATAATTTTCGGCTTAATGGAGCGGTTGATCAATTAAACCTAGAGTTTGGTCTTAGTACCATGTTCATTTATGCCAATTCATATGCGGTCTTCACGAAGATTATTGTCAATTATCGACAATATGGGTTTGTGAATGCAAAGCAACCATGTTGTGTTGGATACTTTCCTCCTTTCATTTGCTACAAGGATCAAAACCAATCATCAAGCTCTTTCTTGTGTGAGGATCGATCTAAATATGTGTTTTGGGATGCATATCACCCAACAGAAGCTGCTAATATCATAATTGCCAAAGAGCTCCTCGATGGAGATGAAACCATTACTTCTCCCATCAACATTCGTCAATTGTATGCCTATCGTTTATAA

Protein sequence

MFEKKMEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSIPFLQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTNEKCLEEVNQLIETYNFRLNGAVDQLNLEFGLSTMFIYANSYAVFTKIIVNYRQYGFVNAKQPCCVGYFPPFICYKDQNQSSSSFLCEDRSKYVFWDAYHPTEAANIIIAKELLDGDETITSPINIRQLYAYRL

Homology

[Close]

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot
Match: Q9FJ25 (GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.2e-66
Identity = 129/183 (70.49%), Postives = 154/183 (84.15%), Query Frame = 0


Query: 30  FTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIGEYLG 89
           FT F+FGDSLVD GNN+YIFTLSKADS PYGIDF PS GQPTGRFTNGRTISDI+GE LG
Sbjct: 24  FTNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPTGRFTNGRTISDIVGEALG 83

Query: 90  AKSFPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLFLGRISLREQVKNFEESRNA 149
           AKS PPP+L P +T+++T I  GINYASGA+GILD+TGLLF+GR+ LREQV NFE+SR  
Sbjct: 84  AKSPPPPYLEP-NTEANT-IRNGINYASGAAGILDDTGLLFIGRVPLREQVSNFEKSREY 143

Query: 150 MVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSIPFLQTNKPSPSDYL-DHMISNLTV 209
           MV+V GEN T E+LKN++F++T+GSNDI+NYIQPSIPF   +K  P+D L D M+ +LT 
Sbjct: 144 MVRVIGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFSQDK-LPTDVLQDSMVLHLTT 203

Query: 210 HLK 212
           HLK
Sbjct: 204 HLK 203

[Close]

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.8e-42
Identity = 90/182 (49.45%), Postives = 124/182 (68.13%), Query Frame = 0


Query: 32  TFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIGEYLGAK 91
           +FVFGDSLVDAGNN+Y+ TLS+A+SPP GIDFKPS G PTGRFTNGRTI+DI+GE LG +
Sbjct: 35  SFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIVGEKLGQQ 94

Query: 92  SFPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLFLGRISLREQVKNFEESRNAMV 151
           S+  P+LAP    S   +  G+NYASG  GIL+ TG +F+ R+ +  QV  F  +R    
Sbjct: 95  SYAVPYLAP--NASGEALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFD 154

Query: 152 KVKGENETME-VLKNSIFSLTVGSNDII-NYIQPSIPFLQTNKPSPSDYLDHMISNLTVH 211
           K+ G+++  + + K S+FS+ +GSND + NY+ P +        +P  ++D MIS+L   
Sbjct: 155 KLLGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQ 214

[Close]

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 1.0e-39
Identity = 97/205 (47.32%), Postives = 130/205 (63.41%), Query Frame = 0


Query: 12  YVVMVIVFFINFFH-----GGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPS 71
           ++  +IV  + FF      G      FVFGDSLVDAGNN+Y+ TLSKA+  P GIDF   
Sbjct: 6   FLCQIIVLSVLFFSEVCLAGKKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDF--- 65

Query: 72  GGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDET 131
            G PTGRFTNGRTI DI+ + LG+    PP+LAP  T S ++I  G+NYASG SGIL+ T
Sbjct: 66  -GSPTGRFTNGRTIVDIVYQALGSDELTPPYLAP--TTSGSLILNGVNYASGGSGILNST 125

Query: 132 GLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDII-NYIQPSI 191
           G LF  RI++  Q+ NF  +R  ++   GE+E  ++ +++IFS+T GSND+I NY  P I
Sbjct: 126 GKLFGERINVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVI 185

Query: 192 PFLQTNKPSPSDYLDHMISNLTVHL 211
             LQ    +P  ++D MIS   + L
Sbjct: 186 STLQRKVVAPEVFVDTMISKFRLQL 204

[Close]

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot
Match: O80470 (GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.3e-38
Identity = 87/188 (46.28%), Postives = 119/188 (63.30%), Query Frame = 0


Query: 26  GGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIG 85
           GG    +F+FGDSLVDAGNN+Y+ TLS+A+  P GIDFK SGG PTGRFTNGRTI DI+G
Sbjct: 43  GGGLGASFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVG 102

Query: 86  EYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLFLGRISLREQVKNFEE 145
           E LG+ ++  PFLAP        +  G+NYASG  GI++ TG +F+ R+ +  QV  F  
Sbjct: 103 EELGSANYAIPFLAP--DAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNT 162

Query: 146 SRNAMVKVKGENETME-VLKNSIFSLTVGSNDII-NYIQPSIP----FLQTNKPSPSDYL 205
           +R     + G+ +  + + K SIFS+T+G+ND + NY+ P +     F QT      D L
Sbjct: 163 TRKQFDDLLGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDML 222

Query: 206 DHMISNLT 208
           +H+   LT
Sbjct: 223 EHLRDQLT 228

[Close]

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 5.1e-31
Identity = 77/179 (43.02%), Postives = 114/179 (63.69%), Query Frame = 0


Query: 33  FVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIGEYLGAKS 92
           FVFGDSLVD GNN+++ +L++++  PYGIDF  +G QPTGRF+NG+TI D IGE LG   
Sbjct: 51  FVFGDSLVDNGNNNHLNSLARSNYLPYGIDF--AGNQPTGRFSNGKTIVDFIGELLGLPE 110

Query: 93  FPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLFLGRISLREQVKNFEESRNAMVK 152
             P F+  +    D  I  G+NYAS A GIL+ETG     R S+  QV+NFE++   + +
Sbjct: 111 I-PAFMDTVDGGVD--ILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISR 170

Query: 153 VKGENETMEVLKNSIFSLTVGSNDII-NYIQPSIPFLQTNKPSPSDYLDHMISNLTVHL 211
              +    E +  S+  +++G+ND I NY++P + FL ++   P+ + D ++SN T HL
Sbjct: 171 SMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRL-FLSSSIYDPTSFADLLLSNFTTHL 223

[Close]

BLAST of Cucsat.G21316 vs. NCBI nr
Match: XP_004143942.3 (GDSL esterase/lipase At5g41890 [Cucumis sativus])

HSP 1 Score: 421 bits (1082), Expect = 6.62e-146
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 0


Query: 1   MFEKKMEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYG 60
           MFEKKMEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYG
Sbjct: 1   MFEKKMEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYG 60

Query: 61  IDFKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGAS 120
           IDFKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGAS
Sbjct: 61  IDFKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGAS 120

Query: 121 GILDETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINY 180
           GILDETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINY
Sbjct: 121 GILDETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINY 180

Query: 181 IQPSIPFLQTNKPSPSDYLDHMISNLTVHLK 211
           IQPSIPFLQTNKPSPSDYLDHMISNLTVHLK
Sbjct: 181 IQPSIPFLQTNKPSPSDYLDHMISNLTVHLK 211

[Close]

BLAST of Cucsat.G21316 vs. NCBI nr
Match: KAE8647829.1 (hypothetical protein Csa_000685 [Cucumis sativus])

HSP 1 Score: 411 bits (1057), Expect = 2.31e-143
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 6   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 65
           MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP
Sbjct: 1   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 60

Query: 66  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 125
           SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 120

Query: 126 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 185
           TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI
Sbjct: 121 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 180

Query: 186 PFLQTNKPSPSDYLDHMISNLTVHLK 211
           PFLQTNKPSPSDYLDHMISNLTVHLK
Sbjct: 181 PFLQTNKPSPSDYLDHMISNLTVHLK 206

[Close]

BLAST of Cucsat.G21316 vs. NCBI nr
Match: XP_008437733.1 (PREDICTED: GDSL esterase/lipase At5g41890 [Cucumis melo])

HSP 1 Score: 391 bits (1005), Expect = 2.95e-134
Identity = 197/206 (95.63%), Postives = 200/206 (97.09%), Query Frame = 0


Query: 6   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 65
           M FSKSYVVMVIVF INFF GG CFTTFVFGDSLVDAGNNDYIFTLSKA+SPPYGIDFKP
Sbjct: 1   MVFSKSYVVMVIVFSINFFDGGLCFTTFVFGDSLVDAGNNDYIFTLSKANSPPYGIDFKP 60

Query: 66  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 125
           SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTII+KGINYASGASGILDE
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIHKGINYASGASGILDE 120

Query: 126 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 185
           TGLLFLGRISLREQVKNFEESRN MVK+KGENETMEVLKNSIFSLTVGSNDIINYIQPSI
Sbjct: 121 TGLLFLGRISLREQVKNFEESRNEMVKLKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 180

Query: 186 PFLQTNKPSPSDYLDHMISNLTVHLK 211
           PF QTNKPSPSDYLDHMISNLTVHLK
Sbjct: 181 PFFQTNKPSPSDYLDHMISNLTVHLK 206

[Close]

BLAST of Cucsat.G21316 vs. NCBI nr
Match: XP_038907274.1 (GDSL esterase/lipase At5g41890 [Benincasa hispida])

HSP 1 Score: 350 bits (898), Expect = 2.63e-117
Identity = 183/209 (87.56%), Postives = 190/209 (90.91%), Query Frame = 0


Query: 4   KKMEFS-KSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGID 63
           KK+ FS KSYVV  IVFFINFF G  CFTTFVFGDSLVD GNNDYIFTLSKADSPPYGID
Sbjct: 46  KKIWFSFKSYVVTFIVFFINFFGGVHCFTTFVFGDSLVDVGNNDYIFTLSKADSPPYGID 105

Query: 64  FKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGI 123
           FKPSGGQPTGRFTNGRTISDIIGEYLGAKSFP PFLAP S ++DTI Y GINYASGASGI
Sbjct: 106 FKPSGGQPTGRFTNGRTISDIIGEYLGAKSFPLPFLAP-SKEADTI-YNGINYASGASGI 165

Query: 124 LDETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQ 183
           LDETGLLFLGRI LREQVKNFE SR+ MVKVKGENETMEVLKN+IFSLTVGSNDIINYIQ
Sbjct: 166 LDETGLLFLGRIPLREQVKNFEASRDYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQ 225

Query: 184 PSIPFLQTNKPSPSDYLDHMISNLTVHLK 211
           PSIPF Q NK SPSDYLDHM+SNLT+HLK
Sbjct: 226 PSIPFFQRNKASPSDYLDHMVSNLTIHLK 252

[Close]

BLAST of Cucsat.G21316 vs. NCBI nr
Match: XP_022977773.1 (GDSL esterase/lipase At5g41890 [Cucurbita maxima])

HSP 1 Score: 334 bits (856), Expect = 1.35e-111
Identity = 171/207 (82.61%), Postives = 190/207 (91.79%), Query Frame = 0


Query: 6   MEFSK-SYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 65
           M+FSK SY++M +VF INFF G  CFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK
Sbjct: 1   MKFSKYSYIMMFVVFSINFFPG-LCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 66  PSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILD 125
           PSGG+PTGRFTNGRTISDIIGEYLGA+SFPPPFLAP +T++DTI Y GINYASGA+GILD
Sbjct: 61  PSGGRPTGRFTNGRTISDIIGEYLGAESFPPPFLAP-NTEADTI-YTGINYASGAAGILD 120

Query: 126 ETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPS 185
           ETG LFLGRI LREQVK+FEESR+ +VKVKGENETM++LK +IFSLT GSNDIINY QPS
Sbjct: 121 ETGFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPS 180

Query: 186 IPFLQTNKPSPSDYLDHMISNLTVHLK 211
           IPF Q+NK +PSDYLDHMISNLT+HLK
Sbjct: 181 IPFFQSNKVAPSDYLDHMISNLTIHLK 204

[Close]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL
Match: A0A0A0KM97 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1)

HSP 1 Score: 411 bits (1057), Expect = 1.71e-142
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 6   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 65
           MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP
Sbjct: 1   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 60

Query: 66  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 125
           SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 120

Query: 126 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 185
           TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI
Sbjct: 121 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 180

Query: 186 PFLQTNKPSPSDYLDHMISNLTVHLK 211
           PFLQTNKPSPSDYLDHMISNLTVHLK
Sbjct: 181 PFLQTNKPSPSDYLDHMISNLTVHLK 206

[Close]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL
Match: A0A1S3AUU3 (GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1)

HSP 1 Score: 391 bits (1005), Expect = 1.43e-134
Identity = 197/206 (95.63%), Postives = 200/206 (97.09%), Query Frame = 0


Query: 6   MEFSKSYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKP 65
           M FSKSYVVMVIVF INFF GG CFTTFVFGDSLVDAGNNDYIFTLSKA+SPPYGIDFKP
Sbjct: 1   MVFSKSYVVMVIVFSINFFDGGLCFTTFVFGDSLVDAGNNDYIFTLSKANSPPYGIDFKP 60

Query: 66  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDE 125
           SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTII+KGINYASGASGILDE
Sbjct: 61  SGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIHKGINYASGASGILDE 120

Query: 126 TGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 185
           TGLLFLGRISLREQVKNFEESRN MVK+KGENETMEVLKNSIFSLTVGSNDIINYIQPSI
Sbjct: 121 TGLLFLGRISLREQVKNFEESRNEMVKLKGENETMEVLKNSIFSLTVGSNDIINYIQPSI 180

Query: 186 PFLQTNKPSPSDYLDHMISNLTVHLK 211
           PF QTNKPSPSDYLDHMISNLTVHLK
Sbjct: 181 PFFQTNKPSPSDYLDHMISNLTVHLK 206

[Close]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL
Match: A0A6J1IR23 (GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 SV=1)

HSP 1 Score: 334 bits (856), Expect = 6.54e-112
Identity = 171/207 (82.61%), Postives = 190/207 (91.79%), Query Frame = 0


Query: 6   MEFSK-SYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 65
           M+FSK SY++M +VF INFF G  CFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK
Sbjct: 1   MKFSKYSYIMMFVVFSINFFPG-LCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 66  PSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILD 125
           PSGG+PTGRFTNGRTISDIIGEYLGA+SFPPPFLAP +T++DTI Y GINYASGA+GILD
Sbjct: 61  PSGGRPTGRFTNGRTISDIIGEYLGAESFPPPFLAP-NTEADTI-YTGINYASGAAGILD 120

Query: 126 ETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPS 185
           ETG LFLGRI LREQVK+FEESR+ +VKVKGENETM++LK +IFSLT GSNDIINY QPS
Sbjct: 121 ETGFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPS 180

Query: 186 IPFLQTNKPSPSDYLDHMISNLTVHLK 211
           IPF Q+NK +PSDYLDHMISNLT+HLK
Sbjct: 181 IPFFQSNKVAPSDYLDHMISNLTIHLK 204

[Close]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL
Match: A0A6J1E0T3 (GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE=3 SV=1)

HSP 1 Score: 328 bits (842), Expect = 7.64e-110
Identity = 171/208 (82.21%), Postives = 185/208 (88.94%), Query Frame = 0


Query: 6   MEFSKSY--VVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDF 65
           MEFS+S+   +M++V  +NF     C TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDF
Sbjct: 1   MEFSRSHFITIMLVVSAMNFL-SCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDF 60

Query: 66  KPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGIL 125
           KPSGGQPTGRFTNGRTISDII EYLGAKSFPPPFLAP +T+ DT+ Y+GINYASGASGIL
Sbjct: 61  KPSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAP-NTEVDTM-YRGINYASGASGIL 120

Query: 126 DETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQP 185
           DETGLLFLGRI LREQVKNFEESR  MVKVKGENETMEVLKN+IFSLTVGSNDIINYIQP
Sbjct: 121 DETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQP 180

Query: 186 SIPFLQTNKPSPSDYLDHMISNLTVHLK 211
           SIP  QTNK SPSDYLDHMISN+T+ LK
Sbjct: 181 SIPIFQTNKASPSDYLDHMISNITIQLK 205

[Close]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL
Match: A0A6J1GD95 (GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=3 SV=1)

HSP 1 Score: 323 bits (828), Expect = 1.16e-107
Identity = 164/201 (81.59%), Postives = 183/201 (91.04%), Query Frame = 0


Query: 11  SYVVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQP 70
           SY+ + +VF INFF G  CFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQP
Sbjct: 7   SYITIFVVFSINFFPG-LCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFKPSGGQP 66

Query: 71  TGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGILDETGLLF 130
           TGRFTNGRTISDIIGEYLGA+SFPPPFLAP +T+SDTI + GINYASGA+GILDETG LF
Sbjct: 67  TGRFTNGRTISDIIGEYLGAESFPPPFLAP-NTESDTI-FTGINYASGAAGILDETGFLF 126

Query: 131 LGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQPSIPFLQT 190
           LGRI LREQVK+F+ESR+ +VKVKGENETM++LK +IFSLT GSNDIINY QPSIPF  +
Sbjct: 127 LGRIPLREQVKHFKESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSIPFFGS 186

Query: 191 NKPSPSDYLDHMISNLTVHLK 211
           NK +PSDYLD+MISNLT+HLK
Sbjct: 187 NKVAPSDYLDYMISNLTIHLK 204

The following BLAST results are available for this feature:

BLAST of Cucsat.G21316 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ25	2.2e-66	70.49	GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3...	[more]
Q9M2R9	3.8e-42	49.45	GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2...	[more]
O23470	1.0e-39	47.32	GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3...	[more]
O80470	3.3e-38	46.28	GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2...	[more]
Q9FNP2	5.1e-31	43.02	GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2...	[more]

BLAST of Cucsat.G21316 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143942.3	6.62e-146	100.00	GDSL esterase/lipase At5g41890 [Cucumis sativus]	[more]
KAE8647829.1	2.31e-143	100.00	hypothetical protein Csa_000685 [Cucumis sativus]	[more]
XP_008437733.1	2.95e-134	95.63	PREDICTED: GDSL esterase/lipase At5g41890 [Cucumis melo]	[more]
XP_038907274.1	2.63e-117	87.56	GDSL esterase/lipase At5g41890 [Benincasa hispida]	[more]
XP_022977773.1	1.35e-111	82.61	GDSL esterase/lipase At5g41890 [Cucurbita maxima]	[more]

BLAST of Cucsat.G21316 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM97	1.71e-142	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1	[more]
A0A1S3AUU3	1.43e-134	95.63	GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1	[more]
A0A6J1IR23	6.54e-112	82.61	GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 ...	[more]
A0A6J1E0T3	7.64e-110	82.21	GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE...	[more]
A0A6J1GD95	1.16e-107	81.59	GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 33..355 e-value: 2.8E-27 score: 96.6
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 31..360 e-value: 6.2E-76 score: 257.8
None	No IPR available	PANTHER	PTHR45648	GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)	coord: 14..373
None	No IPR available	PANTHER	PTHR45648:SF25	GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEIN	coord: 14..373
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 163..356
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 33..359 e-value: 8.29011E-134 score: 383.119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G21316.T1	Cucsat.G21316.T1	mRNA
Cucsat.G21316.T2	Cucsat.G21316.T2	mRNA
Cucsat.G21316.T5	Cucsat.G21316.T5	mRNA
Cucsat.G21316.T3	Cucsat.G21316.T3	mRNA
Cucsat.G21316.T4	Cucsat.G21316.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds