Cucsat.G20916 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G20916
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	alpha/beta-Hydrolases superfamily protein
Location	ctg907: 222488 .. 226449 (-)
RNA-Seq Expression	Cucsat.G20916
Synteny	Cucsat.G20916

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATATAAATCAACCAAGTTGGTGTCCGTGTTGACTGTTTAATCAGGACGTAACATATAGCATATATATCCTCCTAAACCTTGTTCTTATTTTGTTCATTGTGGGGGATGATAAAAGAAAATTTTGAAAAGGGAAATTGGATGGGTTCTAACGAGATACAATTGAATGAAAATGGTCTGACATTAAAGCCAGATAAGGCAAAAGTAAGAGATGTATTGGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGAGCTTTACACCAATTTCAAAGCTCGATGGACCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTCTGCCATCGCTTCTCTTTTCACAATCTTAGTTGCATTTTGGTCAATTATTCAAAAGTTTTGCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTAAGATGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGCAGACATGAATTCACATGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACGGTCCGCCTCCATCGTTGAGTTCGTAGTAAATGACTGCTGGCAGGTACTCCAAAATTAATTTTAATTTTAACTTTTAAGTAGTTGTTTTAAGTGTTCGGTTGAGTTATTTATAAGTTAAAAATTTTGTTTTAGTTTAAGAAAAAGTTACAGAATTTATATGTTTATCCTGAATTAACCCAATTATAGTAAGTTGGTTTTGTTCAAATAATTGTAGGTAAACATTTTAATTGATCGATTACCAAACAAAATTAAGGTCTCGTTTGCTAATCTATTTTAGTTTTAGCTTTATCTTGATTGGCATCTAATTCCTTAAATATAATAATTGAGTTTTTAGCTAAATTTTAAAAACAAAAACTAGTACAAAGGGATTATGAAATAGAAGTTAAGTAATTTTAAAGAAAAACAGCCAACCCTAATGTTAAATTCTGATTGGAATACATAAACTGATGAGTTTTCTTCTTCTCTTTCTGTTTCATTAGTGAATAATATTCATATGCAAGTAAACTTTGCAATATTTATAAACTTTGGGTAATTGGTTATTTGTTATTAGTTTTTCAGAGAACAAAAATTATTTTATAGCTATTTTTGATAAATACGTGCAGATATGTATTTAGAATTTCGTATGTATTGTTCATTTTATGTTGTCTTGTAGTTTTTCTATTTAATCTCATCTCATTTGTAATATTTTACAAGAACACTGAATGCAATATTCAAATCTTCTCTCAAACTTGATACAAATCCAATCCATGATGTTATTTTTTGGTTTTCAAATGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGGTAAGATTTTTTTTTTTTTTGAACTTCTAATTATCAAATTAAATACAAATAAGGACTTTGGAATTCTCTTTTTCAAATAAATAAAGACTCTGAAATGAACTTCTACTTTTCTAACTAAATAAAGACTGTTGACTTCTATATAATTTTGAATAAATAAAGACTTAGCTTGGTCTACTTTTTTAGTTGTATTATAAAAAAAATGTATTTATATTATCAATCATAATAATATTAAAACTTAGGTGCACCTTGTTTAATTTACTATTTTTTAGATTTTAGGGTTTGGAAAGATATTAAAGCTCTTGTCCATCTTTGAGCAAGCAGATCCTCCCCTTTGTTCTCAAACTATACAGCCAATCTTAATATCAACATTTTTGTTCAATTCTACGGATTATGGGGTTTTCTTGGGTTTTTCTCTAGCCATTGATTTTATTTTCGTTGCTTCTATTAATGAAGGGGTAATGATGATGATGCTATGGAGATGTCCACCTAGTGGAGATGTTCGGGTGCACCTGTTGACCTACATCATATTTTTTTTATAAAAAAACTATTTTTTAGAGTTGACCATGAATCAACATTTTATCTTCTTATTTCAATATATATATTTTAAATTAAGATCTTGTTTGGTATATGTACTTTTAGTTTTTGAAAATTGAGTGATAGCAACCTTTTTACATTCTTTTATTATATAAAAGGAAATGATGATAGTGTTATAAATGTCTACCTAGTGGAGATGTTCTAGTGCACGTACTTACTAATATTGTGTTTTCTTTTGGAAAAATAAGCTTTTTAATTTTTTTTGGTCAAGTAAGCATTTATATTTGTTGAATTCTTATCTAAATTCGGGAAAAGAAAAGAATGTTTTTTAAAAACTACATATTTTGCAGACTTTCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACACACAAAGATCCAAACGTAACAGTGATTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGGACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCACTCCGGGTTCATGCAAGCCCTTGGCTATCAAAAGTCCGGTGGTTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGCCATAGTTTGGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCTCAGTTTATGGTCGACACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCCATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGACGTCTTCTACAATGGCAAGGTGACTCTCTACCTATTTGATCATTTATTCGATTTTTTCGTGGTATTAACAACACATTTGTTCAATAATATATTTGTCTCTTTCTCCATCAACACTTAAGCTTTTGAATCAATTCCTTGTTTAAAATTGTATCATAGAACGTGGTCTCTAAAGAGAGGTGATCTTAGACCATAATGCTTTGATATATCATCTCAAGACTTAAATCGCTAATTGATGCAAAAGCTTTGGACTATACGGATGGAGACAAATTTAATATAAAATATAAGAGAATTGTTGTGTAAATTAATTAAATATATATTTTGGTTCATATAACAAATTTAATATAAAATATAAGAGAATTGCTGTGTAAATTAATTAAATATATATTTTGGTTCATATAACAAAAGTTGTTGGATGTGACAATAATCTATATCATTGCTACACTGAAATCTACATTATATTATATAGTGTGATGGTATATCTTTGAATTTAATTTCTTTTATTTTCCATAATTAAAGAGTGAATAAGTTCTTTTTTTCTTCTTCTTTTTTTTGGTGTTATATTTGTATGCAGTTTTTGAAGGAGCAGCCAAACACAAACTACTTTTCACTAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGGTTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAACCTGACTCGTTGGGGTAATATACATTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAGAAAATTAATTGTGTCTGTTTCGATTTTATTTTCTCAAACTACTTTACTCTTCTTTTTCTTTCTTGATTAATTTTTGTTGTAAGGACGTGTTTGTTTGTTTAGTTTGTTGTTCTTACTTTATTTTTAAATTTTGTTAAAAGTATGATATTCGATGATGATCTTTAGATTATTTTTTAATTCCAAATCAATG

Coding sequence (CDS)

ATGATAAAAGAAAATTTTGAAAAGGGAAATTGGATGGGTTCTAACGAGATACAATTGAATGAAAATGGTCTGACATTAAAGCCAGATAAGGCAAAAGTAAGAGATGTATTGGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGAGCTTTACACCAATTTCAAAGCTCGATGGACCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTCTGCCATCGCTTCTCTTTTCACAATCTTAGTTGCATTTTGGTCAATTATTCAAAAGTTTTGCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTAAGATGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGCAGACATGAATTCACATGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACGGTCCGCCTCCATCGTTGAGTTCGTAGTAAATGACTGCTGGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGACTTTCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACACACAAAGATCCAAACGTAACAGTGATTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGGACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCACTCCGGGTTCATGCAAGCCCTTGGCTATCAAAAGTCCGGTGGTTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGCCATAGTTTGGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCTCAGTTTATGGTCGACACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCCATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGACGTCTTCTACAATGGCAAGTTTTTGAAGGAGCAGCCAAACACAAACTACTTTTCACTAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGGTTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAACCTGACTCGTTGGGGTAATATACATTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

Protein sequence

MIKENFEKGNWMGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFAHYIEADY

Homology

[Close]

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.3e-65
Identity = 161/507 (31.76%), Postives = 259/507 (51.08%), Query Frame = 0


Query: 13  GSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQ--IELYTNFKA--- 72
           GSN  Q++ N L ++P +    D+  + +        K +E PD++    +  + +A   
Sbjct: 10  GSNPGQVS-NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 69

Query: 73  RWTISVSILTQKFLSAI--------------ASLFTILVAFWSIIQKFCYKYVLAAEFWV 132
           RW I VSIL +K +  +               +LF+    F+ ++ +     V+  E   
Sbjct: 70  RWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERG- 129

Query: 133 APSRIINFTDVQPRVRC-SDWELLVPDDNADMNSHDR-----DFKYYSALTIMASKLAYQ 192
           + + +     +  R+    +W  +   +  D     R       +    L +MASKLAY+
Sbjct: 130 SVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYE 189

Query: 193 DYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTS 252
               +A +VE VV+  W+M L++  + WND+Q + +T   +F +  KD N+ VI+FRGT 
Sbjct: 190 ----NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTE 249

Query: 253 VLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGY------------------QKSGGWPK 312
             D +DW  D D+SW+ +     +H GF++A+G                    +     K
Sbjct: 250 PFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKK 309

Query: 313 ELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDK 372
            L D     AYY +R  L+ +   ++NA+F++TGHSLGGALA LF TLL  + ET ++ +
Sbjct: 310 NLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKR 369

Query: 373 IQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGG 432
           +  VYTFGQPR+GN+    FM         +Y+R VY  D+VPR+P+     F Y+HFG 
Sbjct: 370 LLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDD-KTFLYKHFGL 429

Query: 433 CVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTI 477
           C+++D FYN    +++P+ N + L + I  ++ A WE +R L +     G  Y EG+F I
Sbjct: 430 CLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTM-GYTHGPDYKEGWFRI 489

[Close]

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.5e-60
Identity = 131/379 (34.56%), Postives = 197/379 (51.98%), Query Frame = 0


Query: 157 LTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDP 216
           L +MASKLAY+    +  +V  VVN  W+M  +D  N WNDF+ + +T   +  +  KD 
Sbjct: 190 LCMMASKLAYE----NEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDA 249

Query: 217 NVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGW--------- 276
           N+ +++FRGT   D +DW+ D D+SW+ +     +H GF++ALG                
Sbjct: 250 NLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFV 309

Query: 277 ----------------PKELTDPKHEF--------------------------------- 336
                           P E +     F                                 
Sbjct: 310 NNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPER 369

Query: 337 -AYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFG 396
            AYY +R  L+ + K + NAKF++TGHSLGGALA LF  +L  H E  +++++  +YT+G
Sbjct: 370 TAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYG 429

Query: 397 QPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFY 456
           QPRVGN+   +FM    +    KY+R VY  DLVPR+P+ +   F ++HFG C Y++  Y
Sbjct: 430 QPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDN-KTFLFKHFGICQYYNSLY 489

Query: 457 NGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLV 477
             + + E+PN NYF + +++P YL+A WE IRS  +   + G +Y E + ++M R +GL 
Sbjct: 490 IEQNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTM-GYMYGSEYEECWESVMLRALGLF 549

[Close]

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.6e-59
Identity = 166/505 (32.87%), Postives = 245/505 (48.51%), Query Frame = 0


Query: 24  LTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTISVSI-----LTQ 83
           L + PD+    D+ + ++ S      K     D ++     + +RW +SV I     L Q
Sbjct: 13  LIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKILQLFQ 72

Query: 84  KFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWV---APSR-------IINFTDVQ--- 143
              + +  L   L+ F+   Q   +  +L   F +    P R        I + D +   
Sbjct: 73  MPAAMLGHLTDFLLNFY--YQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYLDSRMDL 132

Query: 144 ---PRVRCSDWELLVPDDNADM-NSHDRDFK------YYSALTIMASKLAYQDYSRSASI 203
              P V     E++   DN  +   H+   K          L IMASKLAY+    +  +
Sbjct: 133 HGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYE----NTKV 192

Query: 204 VEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWM 263
           VE VV + W+M  +      N FQ+   THA +F +  KD N+ VI+FRGT    I +W 
Sbjct: 193 VERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNWC 252

Query: 264 VDLDFSWFLLEGKVGIHSGFMQALGY--------------QKSGGWPKELTD-----PKH 323
            D DFS   L     +H GF++A+G                KS G   EL       P H
Sbjct: 253 TDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAPDH 312

Query: 324 -----EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQ 383
                +  Y+     L+ + K + NAKF++TGHSLGGALA LF  +L    ET +LD++ 
Sbjct: 313 LVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDRLL 372

Query: 384 AVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCV 443
            VYTFGQPR+GN +   FM +     + +Y+R VY  D+VPR+PF  +  F++ HFG C+
Sbjct: 373 NVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVF-FTFEHFGTCI 432

Query: 444 YFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIME 477
           Y+D  + G F KE+P+ N F +   I  +++A WE  RS I+   V G +Y E + + M 
Sbjct: 433 YYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNH-VYGAEYKETWESRMF 492

[Close]

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.8e-10
Identity = 64/210 (30.48%), Postives = 104/210 (49.52%), Query Frame = 0


Query: 181 NDCWQMKLIDCRNFWNDFQNKA---TTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVD 240
           N C +++  D   F   F++      T  +  +NT+K   + V++FRG+    I +W+ +
Sbjct: 61  NACPEVEKADA-TFLYSFEDSGVGDVTGFLALDNTNK---LIVLSFRGSR--SIENWIGN 120

Query: 241 LDFSWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNA 300
           L+F    L+    I SG     G+  S  W + + D         LRQ + +  + + + 
Sbjct: 121 LNFD---LKEINDICSGCRGHDGFTSS--W-RSVADT--------LRQKVEDAVREHPDY 180

Query: 301 KFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFM-VDTFKT 360
           + + TGHSLGGALAT+    L  +   I       V+++G PRVGN++FA+F+ V T  T
Sbjct: 181 RVVFTGHSLGGALATVAGADLRGNGYDI------DVFSYGAPRVGNRAFAEFLTVQTGGT 237

Query: 361 HDIKYYRYVYSFDLVPRIPFHSLANFSYRH 387
                YR  ++ D+VPR+P      F Y H
Sbjct: 241 ----LYRITHTNDIVPRLP---PREFGYSH 237

[Close]

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 59/171 (34.50%), Postives = 80/171 (46.78%), Query Frame = 0


Query: 221 IAFRGTSVLDINDWMVDLDF---SWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHE 280
           I FRG+S   I +W+ DL F   S+  + G   +H GF+ + G            + ++E
Sbjct: 171 IVFRGSS--SIRNWIADLTFVPVSYPPVSG-TKVHKGFLDSYG------------EVQNE 230

Query: 281 FAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFG 340
                L Q      K   + K  +TGHSLGGA A L   L  Y  E  L      +YT G
Sbjct: 231 LVATVLDQ-----FKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSSSNLFLYTQG 290

Query: 341 QPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFG 389
           QPRVG+ +FA ++V T     I Y R V   D+VP +P    A F + H G
Sbjct: 291 QPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP---PAAFGFLHAG 313

[Close]

BLAST of Cucsat.G20916 vs. NCBI nr
Match: XP_031739748.1 (uncharacterized protein LOC105435389 [Cucumis sativus])

HSP 1 Score: 1032 bits (2669), Expect = 0.0
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0


Query: 1   MIKENFEKGNWMGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIE 60
           MIKENFEKGNWMGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIE
Sbjct: 1   MIKENFEKGNWMGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIE 60

Query: 61  LYTNFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIIN 120
           LYTNFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIIN
Sbjct: 61  LYTNFKARWTISVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIIN 120

Query: 121 FTDVQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVV 180
           FTDVQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVV
Sbjct: 121 FTDVQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVV 180

Query: 181 NDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDF 240
           NDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDF
Sbjct: 181 NDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDF 240

Query: 241 SWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFI 300
           SWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFI
Sbjct: 241 SWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFI 300

Query: 301 ITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIK 360
           ITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIK
Sbjct: 301 ITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIK 360

Query: 361 YYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKY 420
           YYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKY
Sbjct: 361 YYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKY 420

Query: 421 LSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHL 480
           LSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHL
Sbjct: 421 LSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHL 480

Query: 481 PPDHHELLKFAHYIEADY 498
           PPDHHELLKFAHYIEADY
Sbjct: 481 PPDHHELLKFAHYIEADY 498

[Close]

BLAST of Cucsat.G20916 vs. NCBI nr
Match: KGN55200.1 (hypothetical protein Csa_012841 [Cucumis sativus])

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
           SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
           RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 492 HYIEADY 498
           HYIEADY
Sbjct: 481 HYIEADY 487

[Close]

BLAST of Cucsat.G20916 vs. NCBI nr
Match: XP_038896694.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 756 bits (1952), Expect = 1.13e-271
Identity = 363/487 (74.54%), Postives = 414/487 (85.01%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGSNEI+  EN L LK   AKVRDV  FLLPSGSRKIK ++ CPDDQI  YTNFKARWTI
Sbjct: 1   MGSNEIEFRENYLILKAKDAKVRDVFLFLLPSGSRKIKNVIYCPDDQIGFYTNFKARWTI 60

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
           SVSIL QKFL  +A+L T+L AFWSIIQ+F YKY+L AEFWVAP  I NF D++P+VRC 
Sbjct: 61  SVSILAQKFLLTLATLLTLLAAFWSIIQRFFYKYILLAEFWVAPQTI-NFMDLKPKVRCR 120

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DW++L P+DNADMN  +RDF+YYS LTIMASKLAYQDYS+  S+VE VVNDCWQM LIDC
Sbjct: 121 DWKILDPNDNADMNLEERDFRYYSNLTIMASKLAYQDYSQYPSVVESVVNDCWQMNLIDC 180

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
            NFWNDFQ+KATTHA MFENT KDP VTV+AFRGTSVL+ +DW VD +FSW  LEG   I
Sbjct: 181 FNFWNDFQDKATTHAFMFENTSKDPKVTVVAFRGTSVLEPSDWTVDFNFSWSKLEGVGNI 240

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           H+GFMQALG+Q   GWPK+L  PKH++AYY LRQ L++IAK+NDNA+FIITGHSLGGALA
Sbjct: 241 HAGFMQALGFQDLAGWPKDLLQPKHQYAYYTLRQKLKDIAKANDNAEFIITGHSLGGALA 300

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLA+HNET +L KI+AVYT+GQPRVGNQ FAQFMVDT + +  KYYRYVYSFDLV
Sbjct: 301 TLFVTLLAHHNETTILHKIRAVYTYGQPRVGNQEFAQFMVDTVERYGFKYYRYVYSFDLV 360

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PR+PF S+  F Y+HFGGCVYFD FY+GKFLKEQPNTNYFS IW+IPKYLSA WEFIRSL
Sbjct: 361 PRVPFDSVV-FKYKHFGGCVYFDCFYHGKFLKEQPNTNYFSPIWLIPKYLSACWEFIRSL 420

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           IITPIVKGR YFEGFFTI+ERT+GL+IPGISAHVCSNYVN+TR GNI LP D+ E L+ A
Sbjct: 421 IITPIVKGRDYFEGFFTILERTLGLLIPGISAHVCSNYVNVTRLGNIQLPSDYEETLQLA 480

Query: 492 HYIEADY 498
           HYI+A+Y
Sbjct: 481 HYIDANY 485

[Close]

BLAST of Cucsat.G20916 vs. NCBI nr
Match: XP_022936593.1 (uncharacterized protein LOC111443149 [Cucurbita moschata])

HSP 1 Score: 616 bits (1588), Expect = 1.71e-216
Identity = 300/486 (61.73%), Postives = 365/486 (75.10%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
            VSIL QKFL A+A+    L AF S+IQ   + Y+      +        +D  P VRC 
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYM------IGKGEFYTRSDSGPEVRCR 122

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ C
Sbjct: 123 DWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLGC 182

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
            NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   I
Sbjct: 183 FNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRI 242

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           HSGFMQALG QK+ GWPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGALA
Sbjct: 243 HSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGALA 302

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   + +KYYRYVYSFDLV
Sbjct: 303 TLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDLV 362

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PR+PF S+  F Y+HFGGC+YFD FYNGKF KEQPNTNYFSLIWVIPKY SA WE I SL
Sbjct: 363 PRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISSL 422

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           II P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I + PD +E LK  
Sbjct: 423 IIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKLV 473

Query: 492 HYIEAD 497
            YIEAD
Sbjct: 483 RYIEAD 473

[Close]

BLAST of Cucsat.G20916 vs. NCBI nr
Match: KAG6591383.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615 bits (1587), Expect = 2.43e-216
Identity = 300/486 (61.73%), Postives = 364/486 (74.90%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
            VSIL QKFL A+A+    L AF S+IQ   + Y+      +        +D  P VRC 
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYM------IGKGEFYTRSDSGPEVRCR 122

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ C
Sbjct: 123 DWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLGC 182

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
            NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   I
Sbjct: 183 FNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRI 242

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           HSGFMQALG QK+ GWPKEL   +HEFAYY LRQ LR+I KSND AKFI+TGHSLGGALA
Sbjct: 243 HSGFMQALGLQKATGWPKELPGAEHEFAYYTLRQRLRDIVKSNDKAKFIMTGHSLGGALA 302

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   +  KYYRYVYSFDLV
Sbjct: 303 TLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGFKYYRYVYSFDLV 362

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PR+PF S+  F Y+HFGGC+YFD FYNGKF +EQPNTNYFSLIWVIPKYLSA WE I SL
Sbjct: 363 PRVPFDSII-FKYKHFGGCLYFDCFYNGKFRREQPNTNYFSLIWVIPKYLSAHWELISSL 422

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           II P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I + PD +E LK  
Sbjct: 423 IIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKLV 473

Query: 492 HYIEAD 497
            YIEAD
Sbjct: 483 RYIEAD 473

[Close]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL
Match: A0A0A0KZS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE=4 SV=1)

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
           SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
           RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 492 HYIEADY 498
           HYIEADY
Sbjct: 481 HYIEADY 487

[Close]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL
Match: A0A6J1FDN5 (uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC111443149 PE=4 SV=1)

HSP 1 Score: 616 bits (1588), Expect = 8.29e-217
Identity = 300/486 (61.73%), Postives = 365/486 (75.10%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
            VSIL QKFL A+A+    L AF S+IQ   + Y+      +        +D  P VRC 
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYM------IGKGEFYTRSDSGPEVRCR 122

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191
           DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ C
Sbjct: 123 DWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLGC 182

Query: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251
            NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   I
Sbjct: 183 FNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRI 242

Query: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311
           HSGFMQALG QK+ GWPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGALA
Sbjct: 243 HSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGALA 302

Query: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371
           TLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   + +KYYRYVYSFDLV
Sbjct: 303 TLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDLV 362

Query: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431
           PR+PF S+  F Y+HFGGC+YFD FYNGKF KEQPNTNYFSLIWVIPKY SA WE I SL
Sbjct: 363 PRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISSL 422

Query: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491
           II P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I + PD +E LK  
Sbjct: 423 IIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKLV 473

Query: 492 HYIEAD 497
            YIEAD
Sbjct: 483 RYIEAD 473

[Close]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 505 bits (1301), Expect = 2.32e-173
Identity = 256/466 (54.94%), Postives = 319/466 (68.45%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MGS E + + + L LKP++A +  +  F LP G RK++ L++CP  +   YT F  RW I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
            +SIL QKFL AIA+L            K+C    +  +F        + T   P+V C 
Sbjct: 61  FISILLQKFLLAIANLI-----------KYCKS--MRGKF--------HKTCAAPKVNCK 120

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDY-SRSASIVEFVVNDCWQMKLID 191
           +W+++  + N  +   D  FKYY ALT+MAS LAYQDY S   S+V+ VVN CW+M L+ 
Sbjct: 121 EWKIIGMEKNIKLADDDT-FKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLG 180

Query: 192 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 251
           C +FWNDFQNKATT   MF+NT  DPNVTV+AFRG+S  +I DWMVDL+ SW+ +EG   
Sbjct: 181 CYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSS--EIYDWMVDLNLSWYNIEGIGK 240

Query: 252 IHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 311
           IH GFMQALG QK  GWPKEL +P H+FAYY LRQ LR+I KSNDNA+FI TGHSLGGAL
Sbjct: 241 IHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGAL 300

Query: 312 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDL 371
           A LF TLLA+H++  LL+K+QAVYTFGQPRVG++ FAQFM +T + +  KYYRYVYS DL
Sbjct: 301 AVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDL 360

Query: 372 VPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRS 431
           VPR+PF    N  Y HFGGCVYF+  YNGKFL+ QPN NYFS IW+I KY+SA WE IRS
Sbjct: 361 VPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRS 420

Query: 432 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWG 476
           L+I  I     Y EGF  ++ R  GL+ PG SAH+C NY+N  RWG
Sbjct: 421 LMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWG 442

[Close]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 466 bits (1199), Expect = 9.41e-158
Identity = 245/474 (51.69%), Postives = 329/474 (69.41%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71
           MG  + Q + + L LKP++A + D+  F+LP G  +++KL++CP+++ E Y +F  RW I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131
            +SIL+QKFL AIA+L          ++K+     +  +FW    R+  +      V C 
Sbjct: 61  FISILSQKFLLAIANL----------LEKW---EPMRGKFW----RVQTYAS---EVNCR 120

Query: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSR-SASIVEFVVNDCWQMKLID 191
           DW++   ++N  + + D  F YY A+T+MAS L YQD S   +S+V  VVN CW+MKL+ 
Sbjct: 121 DWKIG-KEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLG 180

Query: 192 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 251
           C +F N F++KA T A MF+NT  DPNVTV+AF+GT+   I D ++DL+FSW+ ++ ++G
Sbjct: 181 CYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNT--IYDAILDLNFSWYNVDQRIG 240

Query: 252 -IHSGFMQALGYQKSG-GWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGG 311
            IHSGFM+ALG QK   GWPKEL    H+FAYY+LRQ LR+IAKSND AKFI TGHSLGG
Sbjct: 241 NIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGG 300

Query: 312 ALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSF 371
           ALA LFVT+L+YHNE+ +L+K+QAVYTFGQPRVGN  F +FM  T K +  KYYRYVYS 
Sbjct: 301 ALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSM 360

Query: 372 DLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFI 431
           DLVPRIPF  + +  Y+HFGGCVYF+  Y+G+F++ QPN NYFS +W+IPKYL A WE I
Sbjct: 361 DLVPRIPF-DIFDEWYKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELI 420

Query: 432 RSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPP 482
           RSL++  I     YFEGF T++ R  GL+IPG SAH+C NY+ L R G   +PP
Sbjct: 421 RSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGK--MPP 448

[Close]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 466 bits (1198), Expect = 1.38e-157
Identity = 253/496 (51.01%), Postives = 321/496 (64.72%), Query Frame = 0


Query: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTN-FKARWT 71
           M S E + ++N L LKP  A + D+  FLLP G RK  K ++CP+ + + YT+ F  RW 
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 72  ISVSILTQKFLSAIASLFTILVAFWSIIQKFC---------YKYVLAAEFWVAPSRIINF 131
           I +SI+ Q  L AIA+    L AF   +  F             +L  E  V P    N 
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDE--NS 120

Query: 132 TDVQPRVRCSDWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVN 191
            +    V  +DW      D       +  F+YY  LT+MA++L+Y+    S   V  VVN
Sbjct: 121 AEYTSVVGFADWRR----DLDKSIKAEEIFRYYGGLTVMAAQLSYE----SKPFVLSVVN 180

Query: 192 DCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFS 251
           D W+MKL+   NFWNDFQ KATT A MF+NT  DPNVTV+AFRGTS LD  DW VD DFS
Sbjct: 181 DRWKMKLLGHFNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFS 240

Query: 252 WFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFII 311
           W+ +EG   IHSGFM+ALG QK+ GWP+EL   KH FAYY LRQ LR+IAKSNDNAKFII
Sbjct: 241 WYDIEGVGRIHSGFMKALGLQKATGWPEELVKSKHHFAYYTLRQKLRDIAKSNDNAKFII 300

Query: 312 TGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKY 371
           TGHSLGGALA LFVT+L+ H ++ +L+K+QAVYT+ QPRVG++ FA+FMV+T K +  KY
Sbjct: 301 TGHSLGGALAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKY 360

Query: 372 YRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYL 431
           +RYVYS DLVPR+PF  +  F Y+HFG C+YF+  Y G+ +KE+PN NYFSLIWV+PKYL
Sbjct: 361 HRYVYSSDLVPRVPFDGVI-FKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYL 420

Query: 432 SADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLP 491
           +A WE IRS I+ P+VKG  Y E       R VGL IPG++AH+ ++YVN  R G  + P
Sbjct: 421 NAWWELIRSFIV-PLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 479

Query: 492 PDHHELLKFAHYIEAD 497
           P      +F   IEAD
Sbjct: 481 PQ-----EFEDSIEAD 479

The following BLAST results are available for this feature:

BLAST of Cucsat.G20916 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFU8	3.3e-65	31.76	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
A0A1S3ZP85	2.5e-60	34.56	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	3.6e-59	32.87	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	6.8e-10	30.48	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	1.2e-09	34.50	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of Cucsat.G20916 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739748.1	0.0	100.00	uncharacterized protein LOC105435389 [Cucumis sativus]	[more]
KGN55200.1	0.0	100.00	hypothetical protein Csa_012841 [Cucumis sativus]	[more]
XP_038896694.1	1.13e-271	74.54	triacylglycerol lipase OBL1-like [Benincasa hispida]	[more]
XP_022936593.1	1.71e-216	61.73	uncharacterized protein LOC111443149 [Cucurbita moschata]	[more]
KAG6591383.1	2.43e-216	61.73	Lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cucsat.G20916 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZS7	0.0	100.00	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE...	[more]
A0A6J1FDN5	8.29e-217	61.73	uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC1114431...	[more]
A0A6J1CEU2	2.32e-173	54.94	uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1C148	9.41e-158	51.69	uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CEI9	1.38e-157	51.01	uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 220..377 e-value: 7.6E-35 score: 119.9
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 140..414 e-value: 6.7E-47 score: 161.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 152..405
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 17..481
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 17..481
None	No IPR available	CDD	cd00519	Lipase_3	coord: 151..394 e-value: 1.14878E-40 score: 144.156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G20916.T1	Cucsat.G20916.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity