Cucsat.G20910 (gene) Cucumber (B10) v3

Overview
NameCucsat.G20910
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionabscisic acid 8'-hydroxylase 4-like
Locationctg907: 162412 .. 164926 (-)
RNA-Seq ExpressionCucsat.G20910
SyntenyCucsat.G20910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTGTAACAGACTTTGGGTCTCTGTATCTTCAAAAATGACATCTCCACTTTTTTTTTTTCCCTCCTCTGCCTCAGTATAAAACCCCCCTGCTTTGGCACAATCACTATTATTACAAACCAAATATTTAAAAGAAAAAAAACCATTTAATCAATTTCATATAGAGAAAGATTAAATGGGTGTAGTTTTTCTATCTATTTTGATCTTTTTTCTCACTCTATTATGGTACATTTCTTTAAAGAGAGACAAGAAGGCAGCCATTAGAGATGGAACTAAGCTCCCTCCTGGTTCTTTAGGATGGCCTTATATTGGAGAAACTCTTCAACTCTATTCTCAACACCCCGATTTTTTTTTCGCTGCCAAACAAAAAAGGTTCGAACATTTTCTTTTTCTTTTTCAAAGTTATGAAATATCTTAATTGTTGAACTATGTCCTTTTTGTGTTTCTTTTAAGGTATGGAGAGATTTTTAAGACCCATATACTTGGATGTCCTTGTGTGATGTTGGCTAGTCCTGAGGCAGCGAGATTTGTGTTAGTGACACAGGCTCATTTGTTTAAACCAACATACCCCAAAAGCAAAGAGCTTTTAATTGGCCCATCTGCTCTGTTTTTTCACCAAGGTGATTACCATTGCCGTCTAAGGAAGCTGGTTCAGAGCTCTCTTTCTTTGGACTCCATCCGTACCTTAGTCCCCGATATCGACTCCGTGGCCGCCTCCGCCCTCGACTCCTGGGCCACCTCCGGTGTTATTAACACCTTTCATGAAATGAAAAAGGTTTGTTAATAAGCTTCATCAATCTAGAATCGTTTTTTTAAGAAAATGCATGTCAATTATGACTCAAAGGGTTGTTTTTGTTAATTAATCAGATATCATTTGAAGTTGGGATATTGACCATTTTTGGCCATTTGGAAGCTGCTTACAAAGATGACCTTAAAAAAAACTACAGCATATTGGAAGCAGGCTACAATTCTTTTCCCACCAACATTCCAGGAACTCCATACAAAAAGGCACTCTCAGTAAGTCAAACTTCAGAACAAAAATAAAATAAAATAAAGGCAAAAAGAAAATTTGTCTTATCTTATCTTATTATTGTTATTATTATTATTATTATTATTTTAAAAAAACATATATAAATAAAAGAATTCTGTGTTCTTAACAGGCAAGAAAGAGGCTAAATAAAATCCTAGGTGACATAATTCATGAGAGGGGAGAAAGAAGGCTGAGAGAAAAGGATTTATTGGGGAGCCTTCTCAATTCCATTGATGATGAATGTGAAGTTAAAATAAGTGATTCCCAAATAGCAGATAACATAATAGGAGCGCTTTTTGCTGCTCAAGACACCACTGCCAGTGTCATGACTTGGATTGTTAAGTACCTCCATGACCGTCCCAAGCTTCGTCAATCCATAAAGGTACGTATCGGTATAGTCGACCACCACAATATAACGGACAGTTTATAAAACGAGTAATATTTTGAGTGCACTATGCACTATTCACTATTCACCTACATAATTTTTTTTGGTTTTCTTTCAGGCTGAGCAAGAGGAAATTTTAAAATTAAATTGTGAGAATAATCAAGGGTTAAATTGGGTACAGACAAGGAACATGCCAATCACTTACAAGGTTAATTTTCTGAATGAATTTGTATTATTATTAACTAATAAATGATTTTTAAGAGATTGATTTATGTGGATTTTTTTGTTAAAAACACAGATGGTATTGGAGAGCTTAAGGATGGCCAGCATTATATCATTCACTTTTAGAGAGGCTGTAGCTGATGTGGAATACAAAGGTAAAAGGAAAAAAGAATAAAAAAAAAAAACAGAAAATAAATTGAATTTTATTTCTTTTAGTTTGAACATAATAATATAATAAATAATATGAATTTTTTTGTGAAATGACGTGTAACAGGATACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTTCGGAACATCCATCACAGTCCAGAATATTTTCCTGATCCTCACAATTTCGACCCTTCTAGATTTGAGGTAAAAACAACTCAAACTAAAACTAAACACTTTATAAATTCAAATACTATTTTATTATTTTAATTAAAATGTGGGTTACTGTTCAGGTTGCACCAAAACCCAATACATTTATGCCATTTGGCAGTGGAGTTCATGCCTGCCCAGGAAATGAACTTGCCAAGCTCGAGATCTTGATCATGATTCATCATCTAGTCACCAAGTTCAGGTCAAAACGATTCGTTTAGATAGTGACTATTTGCCTTTTCTTCTTCTTCTACTTTTCGAGAGTAATAACTATTTTTTTTGTTTATTTTTGCTAACTTTGGCAGGTGGGAATTGGTTGGCTCGCAAAACAGGGTTCAGCATAGTCCATTTCCCGTTCCCTTTCAAGGCCTTCCAGCGAGATTTTGGAAGATAGCCCATTAGAAAACTGTAGACGGGAATATGAGCCCGCACTAAGTGGGCCGATTGCCATCCAATCCAAATCCCAGCCCAAGCCCAAGACTTGGGCTGTCGTTTCAAGAATCCATTTTCTCCTCTCCCCTTCTCCATCTGTACTCAAACAGTTTATATTAGTCTAAAATTTTAATTTCATAAATAATCAAAACTTTCAACTAATTATACCAAACTTGTGCTTTATGTTTTTAATCCATGTTTATGCTACCATCATTAATTGGCAGTCTTGCGTACTTTTAATTGACATAGATCTAAAGATTTAAACTGTCGATCTCAAACTATCCTCATGACTTCAACTTATATATACGCCAATTGGGTGTCTATC

Coding sequence (CDS)

ATGGGTGTAGTTTTTCTATCTATTTTGATCTTTTTTCTCACTCTATTATGGTACATTTCTTTAAAGAGAGACAAGAAGGCAGCCATTAGAGATGGAACTAAGCTCCCTCCTGGTTCTTTAGGATGGCCTTATATTGGAGAAACTCTTCAACTCTATTCTCAACACCCCGATTTTTTTTTCGCTGCCAAACAAAAAAGGTATGGAGAGATTTTTAAGACCCATATACTTGGATGTCCTTGTGTGATGTTGGCTAGTCCTGAGGCAGCGAGATTTGTGTTAGTGACACAGGCTCATTTGTTTAAACCAACATACCCCAAAAGCAAAGAGCTTTTAATTGGCCCATCTGCTCTGTTTTTTCACCAAGGTGATTACCATTGCCGTCTAAGGAAGCTGGTTCAGAGCTCTCTTTCTTTGGACTCCATCCGTACCTTAGTCCCCGATATCGACTCCGTGGCCGCCTCCGCCCTCGACTCCTGGGCCACCTCCGGTGTTATTAACACCTTTCATGAAATGAAAAAGATATCATTTGAAGTTGGGATATTGACCATTTTTGGCCATTTGGAAGCTGCTTACAAAGATGACCTTAAAAAAAACTACAGCATATTGGAAGCAGGCTACAATTCTTTTCCCACCAACATTCCAGGAACTCCATACAAAAAGGCACTCTCAGCAAGAAAGAGGCTAAATAAAATCCTAGGTGACATAATTCATGAGAGGGGAGAAAGAAGGCTGAGAGAAAAGGATTTATTGGGGAGCCTTCTCAATTCCATTGATGATGAATGTGAAGTTAAAATAAGTGATTCCCAAATAGCAGATAACATAATAGGAGCGCTTTTTGCTGCTCAAGACACCACTGCCAGTGTCATGACTTGGATTGTTAAGTACCTCCATGACCGTCCCAAGCTTCGTCAATCCATAAAGGCTGAGCAAGAGGAAATTTTAAAATTAAATTGTGAGAATAATCAAGGGTTAAATTGGGTACAGACAAGGAACATGCCAATCACTTACAAGATGGTATTGGAGAGCTTAAGGATGGCCAGCATTATATCATTCACTTTTAGAGAGGCTGTAGCTGATGTGGAATACAAAGGATACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTTCGGAACATCCATCACAGTCCAGAATATTTTCCTGATCCTCACAATTTCGACCCTTCTAGATTTGAGGTTGCACCAAAACCCAATACATTTATGCCATTTGGCAGTGGAGTTCATGCCTGCCCAGGAAATGAACTTGCCAAGCTCGAGATCTTGATCATGATTCATCATCTAGTCACCAAGTTCAGGTGGGAATTGGTTGGCTCGCAAAACAGGGTTCAGCATAGTCCATTTCCCGTTCCCTTTCAAGGCCTTCCAGCGAGATTTTGGAAGATAGCCCATTAG

Protein sequence

MGVVFLSILIFFLTLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSIRTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENNQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWELVGSQNRVQHSPFPVPFQGLPARFWKIAH
Homology
[Close]
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.6e-160
Identity = 273/386 (70.73%), Postives = 320/386 (82.90%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLV QA+LFKPTYPKSKE LIG SA+FFHQGDYH  LRKLVQ+ L+ +SI
Sbjct: 87  MLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQGDYHNHLRKLVQAPLNPESI 146

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           R  +P I+ ++ SAL+SW    V+NT+HEMKK SFEVGIL IFGHL+   K++LKKNYSI
Sbjct: 147 RNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEVGILAIFGHLDGHVKEELKKNYSI 206

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           ++ GYNSFP N+PGT Y+KAL ARK+L KIL +II E  E++  EK LL   LN+ +++ 
Sbjct: 207 VDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKEKKTLEKGLLSCFLNAKEEKG 266

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
            + +++ QIADNIIG LFAAQDTTASV+TWI+KYLHD PKL + +KAEQ+ I + N + N
Sbjct: 267 FLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDNPKLLECVKAEQKVIWQSNEQEN 326

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
            GL W QTR MPIT ++VLE+LRMASIISF FREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 327 HGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVADVEYKGYLIPKGWKVMPLFRNIH 386

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           H+PE+FPDP  FDPSRFE APKPNTFMPFGSGVHACPGNELAKLEILIM HHLVTKFRWE
Sbjct: 387 HNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPGNELAKLEILIMTHHLVTKFRWE 446

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWK 387
           +VGS + +Q+ PFPVP  GL ARFWK
Sbjct: 447 VVGSGSGIQYGPFPVPLGGLAARFWK 472

[Close]
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 538.9 bits (1387), Expect = 4.8e-152
Identity = 257/384 (66.93%), Postives = 314/384 (81.77%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVT AH+FKPTYP+SKE LIGPSALFFHQGDYH  +RKLVQSS   ++I
Sbjct: 81  MLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETI 140

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           R L+PDI+ +A S+L SWA   +++T+ EMKK +F+VGIL IFGHLE++YK+ LK NY+I
Sbjct: 141 RKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIFGHLESSYKEILKHNYNI 200

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           ++ GYNSFP ++PGT Y KAL ARK+L  I+ +II ER E+R  + D LG LLN  +++ 
Sbjct: 201 VDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTDFLGHLLNFKNEKG 260

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
            V ++  QIADNIIG LFAAQDTTAS +TWI+KYLHD  KL +++KAEQ+ I + N    
Sbjct: 261 RV-LTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSREK 320

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           + L W QTRNMP+T+K+++ESLRMASIISFTFREAV DVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 321 KPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIH 380

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           H+P+YF +P  FDPSRFEV PKPNTFMPFGSGVHACPGNELAKL+ILI +HHLV+ FRWE
Sbjct: 381 HNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWE 440

Query: 361 LVGSQNRVQHSPFPVPFQGLPARF 385
           + G +  +Q+SPFP+P  GLPA F
Sbjct: 441 VKGGEKGIQYSPFPIPQNGLPATF 463

[Close]
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.9e-148
Identity = 254/387 (65.63%), Postives = 310/387 (80.10%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYP+SKE +IGPSALFFHQGDYH RLRKLVQ  L  D++
Sbjct: 91  MLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFHQGDYHLRLRKLVQGPLGPDAL 150

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIF-GHLEAAYKDDLKKNYS 120
           R LVPD+++   S L SW    V +TFH MK++SF+VGI+TIF G L+   K +L++NY+
Sbjct: 151 RALVPDVEAAVRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYA 210

Query: 121 ILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDE 180
           I+E GYNSFP + PGT Y KA+ AR+RL+ +L DI+ ER  R     DLLG L+ S   +
Sbjct: 211 IVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGD 270

Query: 181 CEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCEN 240
               ++D Q+ADNIIG LFAAQDTTASV+TWIVKYLHD PKL ++++AEQ  I   N   
Sbjct: 271 DGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGG 330

Query: 241 NQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNI 300
              L W QTR+M +T+K++LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMPLFRNI
Sbjct: 331 RLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNI 390

Query: 301 HHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRW 360
           HH+P+YF DP  FDPSRF+V+P+PNTFMPFG+GVHACPGNELAKLE+L++IHHLVT +RW
Sbjct: 391 HHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRW 450

Query: 361 ELVGSQNRVQHSPFPVPFQGLPARFWK 387
           E+VGS + V++SPFPVP  GL A+ W+
Sbjct: 451 EIVGSSDEVEYSPFPVPKHGLLAKLWR 476

[Close]
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-147
Identity = 253/387 (65.37%), Postives = 310/387 (80.10%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYP+SKE +IGPSALFF+QGDYH RLRKLVQ  L  D++
Sbjct: 91  MLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFNQGDYHLRLRKLVQGPLGPDAL 150

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIF-GHLEAAYKDDLKKNYS 120
           R LVPD+++   S L SW    V +TFH MK++SF+VGI+TIF G L+   K +L++NY+
Sbjct: 151 RALVPDVEAAVRSTLASW-DGNVSSTFHAMKRLSFDVGIVTIFGGRLDERRKAELRQNYA 210

Query: 121 ILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDE 180
           I+E GYNSFP + PGT Y KA+ AR+RL+ +L DI+ ER  R     DLLG L+ S   +
Sbjct: 211 IVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRERRARGEPGSDLLGCLMQSRAGD 270

Query: 181 CEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCEN 240
               ++D Q+ADNIIG LFAAQDTTASV+TWIVKYLHD PKL ++++AEQ  I   N   
Sbjct: 271 DGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDHPKLLEAVRAEQAAIRAANDGG 330

Query: 241 NQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNI 300
              L W QTR+M +T+K++LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMPLFRNI
Sbjct: 331 RLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNI 390

Query: 301 HHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRW 360
           HH+P+YF DP  FDPSRF+V+P+PNTFMPFG+GVHACPGNELAKLE+L++IHHLVT +RW
Sbjct: 391 HHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPGNELAKLEMLVLIHHLVTGYRW 450

Query: 361 ELVGSQNRVQHSPFPVPFQGLPARFWK 387
           E+VGS + V++SPFPVP  GL A+ W+
Sbjct: 451 EIVGSSDEVEYSPFPVPKHGLLAKLWR 476

[Close]
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 3.9e-146
Identity = 248/390 (63.59%), Postives = 311/390 (79.74%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLV+QA LFKPTYP SKE +IGPSALFFHQG+YH RLR+LVQ++L+ DS+
Sbjct: 98  MLASPEAARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSL 157

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIF-GHLEAAYKDDLKKNYS 120
           R LVPD+D+  A+ L +W+   V +TFH MKK+SF+VG++TIF G L   ++++L+ NYS
Sbjct: 158 RALVPDVDAAVAATLAAWSGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYS 217

Query: 121 ILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDD- 180
           ++E GYN FP   PGT Y KA+ ARKRL  IL +I+ ER  R     DLLG L+ S DD 
Sbjct: 218 VVERGYNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDG 277

Query: 181 --ECEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLN 240
                  ++D QIADN++G LFAAQDTTASV+TWI+KYLHD PKL +++KAEQ  I   N
Sbjct: 278 TAGAVALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVAN 337

Query: 241 CENNQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLF 300
               + L W QTR+M +T++++LESLRMASIISFTFREAVADVEYKG+LIPKGWKVMPLF
Sbjct: 338 EGGKRPLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLF 397

Query: 301 RNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTK 360
           RNIHH+P+YF DP  FDPSRF+VAP+P+TF+PFGSGVHACPGNELAKLE+L+++H LVT 
Sbjct: 398 RNIHHNPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTA 457

Query: 361 FRWELVGSQNRVQHSPFPVPFQGLPARFWK 387
           +RWE+VG+ + V++SPFPVP  GL A+ WK
Sbjct: 458 YRWEIVGASDEVEYSPFPVPRGGLNAKLWK 487

[Close]
BLAST of Cucsat.G20910 vs. NCBI nr
Match: XP_004141255.1 (abscisic acid 8'-hydroxylase CYP707A1 [Cucumis sativus] >KGN55192.1 hypothetical protein Csa_011955 [Cucumis sativus])

HSP 1 Score: 792 bits (2046), Expect = 4.08e-288
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI
Sbjct: 82  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 142 RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 202 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN
Sbjct: 262 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 382 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQNRVQHSPFPVPFQGLPARFWKIAH
Sbjct: 442 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 470

[Close]
BLAST of Cucsat.G20910 vs. NCBI nr
Match: XP_008452547.1 (PREDICTED: abscisic acid 8'-hydroxylase 4-like [Cucumis melo])

HSP 1 Score: 771 bits (1990), Expect = 1.39e-279
Identity = 378/389 (97.17%), Postives = 384/389 (98.71%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 82  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 142 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 202 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 262 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 382 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 442 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 470

[Close]
BLAST of Cucsat.G20910 vs. NCBI nr
Match: KAA0064383.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 771 bits (1990), Expect = 2.52e-279
Identity = 378/389 (97.17%), Postives = 384/389 (98.71%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 98  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 157

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 158 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 217

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 218 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 277

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 278 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 337

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 338 QGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 397

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 398 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 457

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 458 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 486

[Close]
BLAST of Cucsat.G20910 vs. NCBI nr
Match: XP_038897319.1 (abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida])

HSP 1 Score: 745 bits (1924), Expect = 1.57e-269
Identity = 365/389 (93.83%), Postives = 378/389 (97.17%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVL+TQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLD+I
Sbjct: 82  MLASPEAARFVLMTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDAI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTL+PDI+SVAASALDSWAT+G+INTFHEMKKISFEVGILTIFGHLEAAYK+DLK NYSI
Sbjct: 142 RTLIPDINSVAASALDSWATAGLINTFHEMKKISFEVGILTIFGHLEAAYKEDLKINYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHER E RL EKDLLGSLLNS+DDE 
Sbjct: 202 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREERLGEKDLLGSLLNSLDDEG 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISD+QIADNIIG LFAAQDTTASVMTWIVKYLHDRPKL QSIKAEQEEILKLNCEN 
Sbjct: 262 EVKISDAQIADNIIGVLFAAQDTTASVMTWIVKYLHDRPKLLQSIKAEQEEILKLNCENG 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           Q LNWVQTRNMPI+YKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QRLNWVQTRNMPISYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFD SRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 382 HSPEYFPDPHNFDSSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 442 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 470

[Close]
BLAST of Cucsat.G20910 vs. NCBI nr
Match: TYK20204.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 739 bits (1909), Expect = 3.65e-267
Identity = 367/389 (94.34%), Postives = 373/389 (95.89%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 98  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 157

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 158 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 217

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 218 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 277

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 278 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 337

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNW           MVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 338 QGLNW-----------MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 397

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 398 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 457

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 458 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 475

[Close]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match: A0A0A0L3K1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 1.97e-288
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI
Sbjct: 82  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 142 RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 202 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN
Sbjct: 262 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 382 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQNRVQHSPFPVPFQGLPARFWKIAH
Sbjct: 442 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 470

[Close]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match: A0A1S3BTH6 (abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3 SV=1)

HSP 1 Score: 771 bits (1990), Expect = 6.72e-280
Identity = 378/389 (97.17%), Postives = 384/389 (98.71%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 82  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 142 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 202 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 262 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 382 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 442 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 470

[Close]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match: A0A5A7V8C1 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00840 PE=3 SV=1)

HSP 1 Score: 771 bits (1990), Expect = 1.22e-279
Identity = 378/389 (97.17%), Postives = 384/389 (98.71%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 98  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 157

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 158 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 217

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 218 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 277

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 278 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 337

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 338 QGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 397

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 398 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 457

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 458 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 486

[Close]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match: A0A5D3D9K5 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003410 PE=3 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 1.77e-267
Identity = 367/389 (94.34%), Postives = 373/389 (95.89%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQ+SLSLDSI
Sbjct: 98  MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSI 157

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           RTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI
Sbjct: 158 RTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 217

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERGERRLREKDLLGSLLNSIDDEC
Sbjct: 218 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDEC 277

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           EVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+PKL QS+KAEQEEILKLN ENN
Sbjct: 278 EVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENN 337

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           QGLNW           MVLESLRMASIIS+TFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 338 QGLNW-----------MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIH 397

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
           HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE
Sbjct: 398 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 457

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFWKIAH
Sbjct: 458 LVGSQNGVQHSPFPVPFQGLPARFWKIAH 475

[Close]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match: A0A6J1IYR9 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 PE=3 SV=1)

HSP 1 Score: 688 bits (1775), Expect = 5.39e-247
Identity = 335/389 (86.12%), Postives = 361/389 (92.80%), Query Frame = 0 

Query: 1   MLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQSSLSLDSI 60
           MLASPEAARFVLVTQAHLFKPTYPKSKE LIGPSALF+HQGDYHCRLRKLVQ+SLSLD I
Sbjct: 82  MLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYHQGDYHCRLRKLVQNSLSLDVI 141

Query: 61  RTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSI 120
           R+LVPDIDSV  SAL SW  + +INTFHEMKKISFEVGIL IFGHLEAAYK+DLKKNYSI
Sbjct: 142 RSLVPDIDSVTTSALHSWTGTQLINTFHEMKKISFEVGILAIFGHLEAAYKEDLKKNYSI 201

Query: 121 LEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERGERRLREKDLLGSLLNSIDDEC 180
           L+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHER E+++ +KDLLG  LNSID+E 
Sbjct: 202 LDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERREKKVGKKDLLGWFLNSIDEEG 261

Query: 181 EVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRPKLRQSIKAEQEEILKLNCENN 240
           E  +SD+QI DNIIG LFAAQDTTASVMTW+VKYL+D+PKL Q++K EQEEIL+ NC+ N
Sbjct: 262 EA-MSDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQPKLLQTLKKEQEEILEFNCQKN 321

Query: 241 QGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 300
           Q LNWVQTRNMP++YK+VLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH
Sbjct: 322 QQLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADVEYKGYLIPKGWKVMPLFRNIH 381

Query: 301 HSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRWE 360
            SPEYF DPH FDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILI+IHHLVTKFRWE
Sbjct: 382 RSPEYFADPHKFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILILIHHLVTKFRWE 441

Query: 361 LVGSQNRVQHSPFPVPFQGLPARFWKIAH 389
           LVGSQN VQHSPFPVPFQGLPARFW IAH
Sbjct: 442 LVGSQNGVQHSPFPVPFQGLPARFWNIAH 469

The following BLAST results are available for this feature:
BLAST of Cucsat.G20910 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A9QNE71.6e-16070.73Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q9LJK24.8e-15266.93Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q0J1851.9e-14865.63Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2121.2e-14765.37Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q09J783.9e-14663.59Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
BLAST of Cucsat.G20910 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004141255.14.08e-288100.00abscisic acid 8'-hydroxylase CYP707A1 [Cucumis sativus] >KGN55192.1 hypothetical... [more]
XP_008452547.11.39e-27997.17PREDICTED: abscisic acid 8'-hydroxylase 4-like [Cucumis melo][more]
KAA0064383.12.52e-27997.17abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
XP_038897319.11.57e-26993.83abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida][more]
TYK20204.13.65e-26794.34abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
BLAST of Cucsat.G20910 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L3K11.97e-288100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1[more]
A0A1S3BTH66.72e-28097.17abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3... [more]
A0A5A7V8C11.22e-27997.17Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3D9K51.77e-26794.34Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1IYR95.39e-24786.12abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002397Cytochrome P450, B-classPRINTSPR00359BP450coord: 80..89
score: 51.56
coord: 89..100
score: 45.37
coord: 54..69
score: 35.14
coord: 9..20
score: 34.63
coord: 26..53
score: 29.13
IPR001128Cytochrome P450PFAMPF00067p450coord: 8..117
e-value: 1.4E-33
score: 116.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 6..140
e-value: 2.3E-42
score: 147.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 9..138
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..139
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 8..139
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 82..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G20910.T1Cucsat.G20910.T1mRNA
Cucsat.G20910.T3Cucsat.G20910.T3mRNA
Cucsat.G20910.T6Cucsat.G20910.T6mRNA
Cucsat.G20910.T2Cucsat.G20910.T2mRNA
Cucsat.G20910.T5Cucsat.G20910.T5mRNA
Cucsat.G20910.T8Cucsat.G20910.T8mRNA
Cucsat.G20910.T7Cucsat.G20910.T7mRNA
Cucsat.G20910.T4Cucsat.G20910.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033075 isoquinoline alkaloid biosynthetic process
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010295 (+)-abscisic acid 8'-hydroxylase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen