Cucsat.G19767 (gene) Cucumber (B10) v3

Overview
NameCucsat.G19767
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionARF guanine-nucleotide exchange factor GNL2
Locationctg4: 796454 .. 802329 (+)
RNA-Seq ExpressionCucsat.G19767
SyntenyCucsat.G19767
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAAGAATGTAAGCGGTGTTTCCATGAAAGAAAATGAGAAATGAGAACAAAAAAAGTTGACAAAGAGAAGAAGAGAACGTACCTTGGAAATTTAAGAGGCGAGCCAAAGCCTGTCCTTGAATCATTCTTCAACCGTCACCAAACCACATGAATCGTTCGAGAGATGAACCATAAAATAATTAAATTGTGAACAAATCTGGAGATTTTAATGGTAGAATCATTCCAATCTTCAATTCCAGCCATTCACATTCCTTCCCATCACCTTAAAAAGTTACTAATAACCCAAACAAATACCTCTCGGTTCCTTCTCTTCCAAATCTTCAACAACAATTCTCATCCTCGATATTCTCTTTCGTGTTCATGGACAAAAGATCATCCGATGACGAAGACGAAAAGGATTCTCCCAAATCCAAACGACGAGAGCTCGGTCTCTCCTGTATGTTAAACACCGAAGTTGGATCCATCCTCGCCGTTATCCGCCGTCCACCTTCCGAATTAAACTCTCCTTATATCTCCACCATTGATGAAACCTACGATTCAACAATCCAACAATCCTTAAAATCCCTTCGAGCATTAATCTTCCACCCTCAACAAAAATGGCGAACCATCGACCCTTCCATCTACATCTCTCCTATTCTCGACGTTATACAAAGCGACGACATTCCCGCTGCGGCCACTGGCGTCGCTCTCTCTGCATTGCTCAAAATAATAAAGGTGGAAATCTTCGACGAGAAGACTCCAGGTGCTAAAGACGCAATAAATTTAATAGTAATTGGAATCACAAATTGCAAACTTGAAAAAACTGACCTAGTAACCGAAGATGCCGTGATGATGAAGGTTCTACAAGTTCTTGCTGGATTGATGAACCACAGAGCTTCATACCTGTTAAATGATCAATCTGTGTGTACAATTGTGAACACATGTTTCAATGTGGTTCAGCAATCGGCGAGTAGAGGGGATTTGCTGCAGAGGACGGCGAGGTACACGATGAATGAGCTGATACAGATAATATTTTCGAGGTTGCCGGAGATAGAGGTGAGAGACGGGGAGGAGTCGGAGTCGGATACGGAGGATGCGGATTTAGGAGGGAGTTTGGATTCAGGGTATGGGATAAGGTGTGTGATAGATGTGTTTCATTTTTTGTGTTCGTTGTTGAATGTGGTGGAAATAATGGTGGAGGTGGGGGATGGAGGGTTAGGATCAAGGACAGCGGATGAGGATGTTCAATTGTTTGCTTTGGTGTTGATAAACTCGGCGGTGGAATTGAGTGGAGACGCCATTGGTAAACATCCAAAGCTTTTGAGGATGGTTCAGGATGATCTGTTTCATCATTTGATTCATTATGGAGCAGCTTCCAATCCTTTGGTTTTGTCCATGATCTGCAGTACAGTTTTGAATATCTATCACTTCCTTCGAAGGTAAACACCCTCTTCCAGCTTTTAAAAATCCCCGCCTTACCTTATAGCTACTTATACAAGAGATTATTGGTTCAAGCTAATATAAACCACCTACCTAGAAATTAAACATTCATCCATGTCAAAAAAATCGTGACTTGCTTTTTTTTTTGTTCTTCTAATATAGAGAGAACTTACTTGTAGACTAATGTTTAGCAATTGAATTATTCAGGATTTATTTATTTTTAAGTTTAATAATATGGGGGAGTGATTCCTCTTGGTTTCAAAATTAAAAGTATAATATGCATCAACTCTTTTTAGTTACGTGAAGATTAGCATTGTAATAACTTATTCTTCCTCAAAAGTTTTAGGAGCTGTTTTTCTAAAACTATATTTATTAAAGAAATAGTTGCAAATTTAGCAATCAAATTCAAATTAATCAAGTATATAGCAACATTTTAAAAAATTTGCATTTTAAAAAATTTGCAAATAGAGCAAAATTTGTCCAATTCTATCAATGATATAAGTCTATCAGTGATAGTTTTGCTATATTTATAATTTTAAAAAAATGTTGTTATATCCTTAATTATTATTGTTAAAATAGTCATCAATCTCAATTTTGTTTGAGTGAAAGTTGTACTTTATTTTGGGTTAAAAGTATTTGGGCGTAGTCCATAAAAGAAAAGCCCAATTTCTAAAAGTTTCAAATATTAGAATATTCTAGAAAAATTATATTATGTTAGAAAAAAATGTAACAGTAAAAGAATAGTCTAGGAAAACATATATAATCACTCAATCAGGAACGTACTGGCAAAATCGTAACTATAAAGTCATAAATTTAGAGGTATCAACTAGTATAAACAATAGAGATATTTTAGAATTTCCATTAATAAGATGCGATCAATAACACAAAAGACATCTTTCTTTAACTCTTTTAACAATATGGGAAAAGTATATGAGCAACTCACTTGAAGATAAATGTAATTATTTAACCGAAGAAACTATTATGCTTAAATTGATTAAAACAATGTTGAACTTTAATGGAATGTAACAAGAAAATAATTTCTAATTCTTCCTCCTACAAAATATTTCTGCAATTGTATAGACTATGAGAGAAGTATGGTCATGCGGAAGGCAAATATATCTCTCAACATTTCCCTAATACATGAACTCAATACCTAAACAAATGAAAAGAGAGAATAAAGAGAAACCAAATAACTAATATTTTTCTATTTCGTCAATCTTTGGACAGGTTTGTTCGACTTCAGTTAGAATCATTCTTCGTCTATGTGGCATTGAAATTAGCATCATTTGGGAACTCAACCCAAATCCAAGAAGTTGCACTTGAAGGAATCATAAACTTCTGTAGACAGTCTTCCTTCATTCTAGAATTCTATGTAAACTATGACTGCGATCCCCTTCGTTGGAATTTGCTCGAGGAGATCGGGAAGTTGCTATGTAAGCTCTCATTTCCTACAGGAAGTCCCTTAACCACTTTAAATATCCAAGCCTTCGAGGGACTAGTGATTGTGATTCACAACATTGCAGAAAAACTTGATAAACATAAAGAAGAAACTTGTGGGGGCGGCGGCAATTTAAGAGTATATCCAGCTCAAGTCGACGAATATATACCGTTTTGGGAAGAAAAATCTAAAGAAGACTTAGACTTGGAGGATTGGCTACGTTATGTAAGAGTAAGAAAGGCACAGAAGAAGAAGATATTGATCGCTGGTCACCATTTCAATAGAGATGAAAAGAAAGGCTTAGCATATTTGAAGCTTTCTCTGTTGGTTTCTGATCCTCCGGATCCGAAAGCATATGCTTACTTTTTCAGGTACACTCATGGATTGGACAAACAGTTTGTTGGTGAATATCTTGGTGATCCTGGTCAGTTTCATGTAAAAGTGTTGGCTGAGTTTACAGAGACCTTTGAATTCACAGGCATGATTCTAGACACTGCTCTTAGAACTTACCTCGAGACGTTTCGATTGCCGGGAGAAGCGCAAAAGATTCATCGCATATTAGAGGCGTTTTCAGAGAGATTTTATGAACTACAATCTTCAAATACCTTCGCGAGTAAAGATACAGTGTTTGTTCTTTGCTATTCTTTAATTATGTTGAATACTGATCAACATAATCCACAAGTGAAGAAAAAGATGACCGAAGACGAGTTCATCAGAAACAACAGAGAAATTAATGCAGGGAAGGATCTTCCTAGAGATTATTTATCTGAGCTTTTTCATTCGATTTCGAATAATGCAATTATACTCTCACCACAGTCAGGTTTGCAACTTGATATGAACCCTAGTAAATGGGTTGAATTGATGAATAGATCCAAGATTATACAACCATTCATGTCGTATGATTTTGACCCTCGCCTGGGAAGAGACATGTTTGGCTGCATTGCTGGTCCTTCTGTTGCATCTCTTGCAGCTTTCTTTGAGCATGCGGATGAAGATGAGATGCTGAATGAATGCATTGAAGGATTGTTTTCCATTGCTAAGATAACACAGTATGGATTAGAGGACACGCTCGATGAACTGCTGGCAATGTTTTGCAAATTTACCACTCTATTGAACCCTTATGCATCTGCAGAAGAAACACTTTTTGTATTTAGTCATGATATGAAGCCAAAACTGGCAACGCTAGCAGTTTTCACGATTGCAAACAACTTCGGTGACACAATTCGAGGAGGTTGGAGAAACATTGTGGATTGCCTACTAAAACTCAAAAGACTTAAACTACTTCCCCAATCAGTTATTGACTTCGAAGTAGCTTCCACCTCATCAAATGATGTTGCAAGGTCAGATTCAGGTGTGATTTTCCCATCTCAAGATCCTAAATTTTGCACCCAGCAGTCCTCTGGTATGGTCAGTCGGTTTTCGCAGTTTTTATCACTTGATAGTATGGAAGATTCCTTAACTCTCAACTTAAATGAATATGAACAAAATTTGAAGTTTATCAAGCAATGTCGCATCGGAAACATCTTTAGCAACAGTTCAAACATACTTGATGAGGCTCTACTCAATCTTGGGCGGTCCCTAATATTTGCCGCTGCTGGAAAAGGACAGAAGTTTAGCACACCAATTGAAGAAGAAGAAACTGTCGGATTTTGTTGGGATCTAATTATAACAATGACCATGGCTAACCTATACCGATTCCAAGTTTTCTGGCCTAACTTCCATGAGTATCTACAAACAGTTGTTCAATTTCCTCTTTTCTCTGCCATCCCATTTGCAGAAAAGGCTGTTTTAGGACTTTTCAAGGTTTGTCTAAGGCTCCTTTCGACATACCAACCTGACAAAATACCTGAGGAACTCATCTTCAAGTCCATAAATTTGATGTGGATGCTTGATAAGGAGATTCTTGATACTTGCTTTGAGTCCATTACACAATCAGTAAGCAAGATTCTTATTGAATACCCAGCAAATCTCCAAAGCCAAATTGGTTGGAAGTCACTGCTTCATTTGTTGTCAGCCACTGGTCGGCATCCCGAAACCTATGACCAAGGAGTCGAGACCTTGATCATGTTGATGTCTGATGCGTCACATATAACACGCACAAACTATACCTTCTGTATAGATTGCGCCTTTAGTTATGTTGCACTCAAGAATAGCCCGTTAGACAAAAACTTGAAAATCTTAGATGCATTATCAGATTCTGTAAATTTCTTAGTCCAATGGTACCGGAACTACTGTGCAGAATCAGGGAATAGTTTTAGTGTAGCAAGCAATGCAAGTAGCTCCTCATTAGATGAAAAAGGTTTAGGATCTTCTAATTTTGCCTTGACTTTATTTCTCAAACTAGGCGAAGCACTTCGAAAAACGAGCTTAGCTAGAAGAGAAGAGATAAGAAACCATGCCATTACATCTCTTAAGAAAAGCTTTGTGTTGGCAGAAGAATTGGATTTCCCTCCAACCAACTGTATTGGTTGTTTTAACAATATAATTTTTGCAATGGTGGATGATTTGCATGAAAAAATGTTGGAGTACTCTAGAAGAGATAATGCAGAGAGGGAGGCAAGAAGCATGGATGGTACTCTAAAGATTTCAATGGAGCTCTTGACAGATGTTTACTTGGTATACTTGAAACAGATATCAGAAAGCCCTGGGTTCCGGACCTTTTGGCTTGGGGTATTGAGAAGAATGGATACCTGTATGAAGGCTGATCTAGGAAGTTATGGTGAATCAAGTTTGAAAGACTTAATCCCAGAATTATTGAGAAAAATAATCACAACTATGAGAGAAAAAGAAATATTGGTGAAGAAAGAAGGTGAAGATCTGTGGGAAATAACTTACATCCAGATCCAGTGGATTGCTCCAGGCATCAAAGACGAGCTTTTCCCAGAAGAATGCTTTTGAACTTGTGACATATTACTTGTAAAATAATAGATTAACTTTAACTCACTGATAATTTACCTTTGTTTTTGCTCTTAGAAAGTAATCGGAGGAATTTTTCTACCTTTTTTTTTATAGGATTAT

Coding sequence (CDS)

ATGGACAAAAGATCATCCGATGACGAAGACGAAAAGGATTCTCCCAAATCCAAACGACGAGAGCTCGGTCTCTCCTGTATGTTAAACACCGAAGTTGGATCCATCCTCGCCGTTATCCGCCGTCCACCTTCCGAATTAAACTCTCCTTATATCTCCACCATTGATGAAACCTACGATTCAACAATCCAACAATCCTTAAAATCCCTTCGAGCATTAATCTTCCACCCTCAACAAAAATGGCGAACCATCGACCCTTCCATCTACATCTCTCCTATTCTCGACGTTATACAAAGCGACGACATTCCCGCTGCGGCCACTGGCGTCGCTCTCTCTGCATTGCTCAAAATAATAAAGGTGGAAATCTTCGACGAGAAGACTCCAGGTGCTAAAGACGCAATAAATTTAATAGTAATTGGAATCACAAATTGCAAACTTGAAAAAACTGACCTAGTAACCGAAGATGCCGTGATGATGAAGGTTCTACAAGTTCTTGCTGGATTGATGAACCACAGAGCTTCATACCTGTTAAATGATCAATCTGTGTGTACAATTGTGAACACATGTTTCAATGTGGTTCAGCAATCGGCGAGTAGAGGGGATTTGCTGCAGAGGACGGCGAGGTACACGATGAATGAGCTGATACAGATAATATTTTCGAGGTTGCCGGAGATAGAGGTGAGAGACGGGGAGGAGTCGGAGTCGGATACGGAGGATGCGGATTTAGGAGGGAGTTTGGATTCAGGGTATGGGATAAGGTGTGTGATAGATGTGTTTCATTTTTTGTGTTCGTTGTTGAATGTGGTGGAAATAATGGTGGAGGTGGGGGATGGAGGGTTAGGATCAAGGACAGCGGATGAGGATGTTCAATTGTTTGCTTTGGTGTTGATAAACTCGGCGGTGGAATTGAGTGGAGACGCCATTGGTAAACATCCAAAGCTTTTGAGGATGGTTCAGGATGATCTGTTTCATCATTTGATTCATTATGGAGCAGCTTCCAATCCTTTGGTTTTGTCCATGATCTGCAGTACAGTTTTGAATATCTATCACTTCCTTCGAAGGTTTGTTCGACTTCAGTTAGAATCATTCTTCGTCTATGTGGCATTGAAATTAGCATCATTTGGGAACTCAACCCAAATCCAAGAAGTTGCACTTGAAGGAATCATAAACTTCTGTAGACAGTCTTCCTTCATTCTAGAATTCTATGTAAACTATGACTGCGATCCCCTTCGTTGGAATTTGCTCGAGGAGATCGGGAAGTTGCTATGTAAGCTCTCATTTCCTACAGGAAGTCCCTTAACCACTTTAAATATCCAAGCCTTCGAGGGACTAGTGATTGTGATTCACAACATTGCAGAAAAACTTGATAAACATAAAGAAGAAACTTGTGGGGGCGGCGGCAATTTAAGAGTATATCCAGCTCAAGTCGACGAATATATACCGTTTTGGGAAGAAAAATCTAAAGAAGACTTAGACTTGGAGGATTGGCTACGTTATGTAAGAGTAAGAAAGGCACAGAAGAAGAAGATATTGATCGCTGGTCACCATTTCAATAGAGATGAAAAGAAAGGCTTAGCATATTTGAAGCTTTCTCTGTTGGTTTCTGATCCTCCGGATCCGAAAGCATATGCTTACTTTTTCAGGTACACTCATGGATTGGACAAACAGTTTGTTGGTGAATATCTTGGTGATCCTGGTCAGTTTCATGTAAAAGTGTTGGCTGAGTTTACAGAGACCTTTGAATTCACAGGCATGATTCTAGACACTGCTCTTAGAACTTACCTCGAGACGTTTCGATTGCCGGGAGAAGCGCAAAAGATTCATCGCATATTAGAGGCGTTTTCAGAGAGATTTTATGAACTACAATCTTCAAATACCTTCGCGAGTAAAGATACAGTGTTTGTTCTTTGCTATTCTTTAATTATGTTGAATACTGATCAACATAATCCACAAGTGAAGAAAAAGATGACCGAAGACGAGTTCATCAGAAACAACAGAGAAATTAATGCAGGGAAGGATCTTCCTAGAGATTATTTATCTGAGCTTTTTCATTCGATTTCGAATAATGCAATTATACTCTCACCACAGTCAGGTTTGCAACTTGATATGAACCCTAGTAAATGGGTTGAATTGATGAATAGATCCAAGATTATACAACCATTCATGTCGTATGATTTTGACCCTCGCCTGGGAAGAGACATGTTTGGCTGCATTGCTGGTCCTTCTGTTGCATCTCTTGCAGCTTTCTTTGAGCATGCGGATGAAGATGAGATGCTGAATGAATGCATTGAAGGATTGTTTTCCATTGCTAAGATAACACAGTATGGATTAGAGGACACGCTCGATGAACTGCTGGCAATGTTTTGCAAATTTACCACTCTATTGAACCCTTATGCATCTGCAGAAGAAACACTTTTTGTATTTAGTCATGATATGAAGCCAAAACTGGCAACGCTAGCAGTTTTCACGATTGCAAACAACTTCGGTGACACAATTCGAGGAGGTTGGAGAAACATTGTGGATTGCCTACTAAAACTCAAAAGACTTAAACTACTTCCCCAATCAGTTATTGACTTCGAAGTAGCTTCCACCTCATCAAATGATGTTGCAAGGTCAGATTCAGGTGTGATTTTCCCATCTCAAGATCCTAAATTTTGCACCCAGCAGTCCTCTGGTATGGTCAGTCGGTTTTCGCAGTTTTTATCACTTGATAGTATGGAAGATTCCTTAACTCTCAACTTAAATGAATATGAACAAAATTTGAAGTTTATCAAGCAATGTCGCATCGGAAACATCTTTAGCAACAGTTCAAACATACTTGATGAGGCTCTACTCAATCTTGGGCGGTCCCTAATATTTGCCGCTGCTGGAAAAGGACAGAAGTTTAGCACACCAATTGAAGAAGAAGAAACTGTCGGATTTTGTTGGGATCTAATTATAACAATGACCATGGCTAACCTATACCGATTCCAAGTTTTCTGGCCTAACTTCCATGAGTATCTACAAACAGTTGTTCAATTTCCTCTTTTCTCTGCCATCCCATTTGCAGAAAAGGCTGTTTTAGGACTTTTCAAGGTTTGTCTAAGGCTCCTTTCGACATACCAACCTGACAAAATACCTGAGGAACTCATCTTCAAGTCCATAAATTTGATGTGGATGCTTGATAAGGAGATTCTTGATACTTGCTTTGAGTCCATTACACAATCAGTAAGCAAGATTCTTATTGAATACCCAGCAAATCTCCAAAGCCAAATTGGTTGGAAGTCACTGCTTCATTTGTTGTCAGCCACTGGTCGGCATCCCGAAACCTATGACCAAGGAGTCGAGACCTTGATCATGTTGATGTCTGATGCGTCACATATAACACGCACAAACTATACCTTCTGTATAGATTGCGCCTTTAGTTATGTTGCACTCAAGAATAGCCCGTTAGACAAAAACTTGAAAATCTTAGATGCATTATCAGATTCTGTAAATTTCTTAGTCCAATGGTACCGGAACTACTGTGCAGAATCAGGGAATAGTTTTAGTGTAGCAAGCAATGCAAGTAGCTCCTCATTAGATGAAAAAGGTTTAGGATCTTCTAATTTTGCCTTGACTTTATTTCTCAAACTAGGCGAAGCACTTCGAAAAACGAGCTTAGCTAGAAGAGAAGAGATAAGAAACCATGCCATTACATCTCTTAAGAAAAGCTTTGTGTTGGCAGAAGAATTGGATTTCCCTCCAACCAACTGTATTGGTTGTTTTAACAATATAATTTTTGCAATGGTGGATGATTTGCATGAAAAAATGTTGGAGTACTCTAGAAGAGATAATGCAGAGAGGGAGGCAAGAAGCATGGATGGTACTCTAAAGATTTCAATGGAGCTCTTGACAGATGTTTACTTGGTATACTTGAAACAGATATCAGAAAGCCCTGGGTTCCGGACCTTTTGGCTTGGGGTATTGAGAAGAATGGATACCTGTATGAAGGCTGATCTAGGAAGTTATGGTGAATCAAGTTTGAAAGACTTAATCCCAGAATTATTGAGAAAAATAATCACAACTATGAGAGAAAAAGAAATATTGGTGAAGAAAGAAGGTGAAGATCTGTGGGAAATAACTTACATCCAGATCCAGTGGATTGCTCCAGGCATCAAAGACGAGCTTTTCCCAGAAGAATGCTTTTGA

Protein sequence

MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDSTIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDADLGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIPFWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLDELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLSLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQKFSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVLGLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPEECF
Homology
BLAST of Cucsat.G19767 vs. ExPASy Swiss-Prot
Match: F4K2K3 (ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=GNL2 PE=2 SV=1)

HSP 1 Score: 1843.6 bits (4774), Expect = 0.0e+00
Identity = 908/1382 (65.70%), Postives = 1139/1382 (82.42%), Query Frame = 0

Query: 16   KSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETY-DSTIQQSLKSLRALIF 75
            ++KR+ELG+SCMLNTEVG++LAVIRRP SE    Y+S  +  + DS++QQSLKSLRALIF
Sbjct: 7    RAKRKELGISCMLNTEVGAVLAVIRRPLSE---SYLSPQETDHCDSSVQQSLKSLRALIF 66

Query: 76   HPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAIN 135
            +PQQ WRTIDPS+Y+SP L+VIQSD+IPA+AT VALS++LKI+K+EIFDEKTPGAKDA+N
Sbjct: 67   NPQQDWRTIDPSVYLSPFLEVIQSDEIPASATAVALSSILKILKIEIFDEKTPGAKDAMN 126

Query: 136  LIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQ 195
             IV GIT+C+LEKTDLV+EDAVMM++LQVL G+M H +S LL DQ+VCTIVNTCF VVQQ
Sbjct: 127  SIVSGITSCRLEKTDLVSEDAVMMRILQVLTGIMKHPSSELLEDQAVCTIVNTCFQVVQQ 186

Query: 196  SASRGDLLQRTARYTMNELIQIIFSRLPEIEVR---DGEESESDTEDADLGGSLDSGYGI 255
            S  RGDLLQR  RYTM+ELIQIIFSRLP+ EVR    GE+SESDT++ D+ G    GYGI
Sbjct: 187  STGRGDLLQRNGRYTMHELIQIIFSRLPDFEVRGDEGGEDSESDTDEIDMSG----GYGI 246

Query: 256  RCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHP 315
            RC ID+FHFLCSLLNVVE++  +   G    TADEDVQ+FALVLINSA+ELSGDAIG+HP
Sbjct: 247  RCCIDIFHFLCSLLNVVEVVENL--EGTNVHTADEDVQIFALVLINSAIELSGDAIGQHP 306

Query: 316  KLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVYVALKLA 375
            KLLRMVQDDLFHHLIHYGA+S+PLVLSMICS +LNIYHFLR+F+RLQLE+FF +V L++ 
Sbjct: 307  KLLRMVQDDLFHHLIHYGASSSPLVLSMICSCILNIYHFLRKFMRLQLEAFFSFVLLRVT 366

Query: 376  SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLSFPTGSP 435
            +F     +QEVALEG+INFCRQ +FI+E YVNYDCDP+  N+ EE GK+LC+ +FPT  P
Sbjct: 367  AFTGFLPLQEVALEGLINFCRQPAFIVEAYVNYDCDPMCRNIFEETGKVLCRHTFPTSGP 426

Query: 436  LTTLNIQAFEGLVIVIHNIAEKLDKHK----EETCGGGGNLRVYPAQVDEYIPFWEEKSK 495
            LT++ IQAFEGLVI+IHNIA+ +D+ +    EE       ++  P ++ EYIPFW +K K
Sbjct: 427  LTSIQIQAFEGLVILIHNIADNMDREEDEGNEEDDNNSNVIKPSPVEIHEYIPFWIDKPK 486

Query: 496  EDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYFFR 555
            E  D E W+ ++RVRKAQK+K+ IA +HFNRDEKKGL YLK + LVSDP DP A A FFR
Sbjct: 487  E--DFETWVDHIRVRKAQKRKLAIAANHFNRDEKKGLEYLKYNYLVSDPLDPMALASFFR 546

Query: 556  YTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIH 615
            +T GLDK  +G+YLGDP + H+ VL  FT TFEFTGM LDTALRT+LE+FRLPGE+QKI 
Sbjct: 547  FTPGLDKTMIGDYLGDPDELHLSVLRSFTHTFEFTGMNLDTALRTFLESFRLPGESQKIE 606

Query: 616  RILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREI 675
            R++EAFSERFY+ QSS+ FASKDTV +LCYSLIMLNTDQHNPQV++KMTEDEFIRNNR I
Sbjct: 607  RMIEAFSERFYDQQSSDIFASKDTVHILCYSLIMLNTDQHNPQVRRKMTEDEFIRNNRAI 666

Query: 676  NAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSYDFDP 735
            NAG DLP++YLSELF SI+ NA  LS  SG  ++MNP++W+ELMNR+K  QPF    FD 
Sbjct: 667  NAGNDLPKEYLSELFQSIATNAFALSTHSG-PVEMNPNRWIELMNRTKTTQPFSLCQFDR 726

Query: 736  RLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLDELLAMF 795
            R+GRDMF  IAGPS+A+++AFFEH+D+DE+L+EC++ + SIA++ QYGLED LDEL+A F
Sbjct: 727  RIGRDMFATIAGPSIAAVSAFFEHSDDDEVLHECVDAMISIARVAQYGLEDILDELIASF 786

Query: 796  CKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKR 855
            CKFTTLLNPY + EETLF FSHDMKP++ATLAVFT+AN FGD+IRGGWRNIVDCLLKL++
Sbjct: 787  CKFTTLLNPYTTPEETLFAFSHDMKPRMATLAVFTLANTFGDSIRGGWRNIVDCLLKLRK 846

Query: 856  LKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLSLDSMED 915
            L+LLPQSVI+FE+     N  + SD   +  SQD KF  +Q S ++ RFS FL+LD++E+
Sbjct: 847  LQLLPQSVIEFEI--NEENGGSESDMNNV-SSQDTKFNRRQGSSLMGRFSHFLALDNVEE 906

Query: 916  SLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQKFSTPIEE 975
            S+ L ++E+EQNLK IKQCRIG IFS SS + D A+LNLGRSLI+AAAGKGQKFST IEE
Sbjct: 907  SVALGMSEFEQNLKVIKQCRIGQIFSKSSVLPDVAVLNLGRSLIYAAAGKGQKFSTAIEE 966

Query: 976  EETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVLGLFKVCL 1035
            EETV FCWDLIIT+ ++N++RF +FWP++HEYL  V  FPLFS IPF EK + GLF+VC+
Sbjct: 967  EETVKFCWDLIITIALSNVHRFNMFWPSYHEYLLNVANFPLFSPIPFVEKGLPGLFRVCI 1026

Query: 1036 RLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANLQSQIGWK 1095
            ++L++   D +PEELIF+S+ +MW +DKEI++TC+++IT+ VSKI+I+Y ANL + IGWK
Sbjct: 1027 KILASNLQDHLPEELIFRSLTIMWKIDKEIIETCYDTITEFVSKIIIDYSANLHTNIGWK 1086

Query: 1096 SLLHLLSATGRHPETYDQGVETLIMLMS-DASHITRTNYTFCIDCAFSYVALKNSPLDKN 1155
            S+L LLS  GRHPET +Q V+ LI LMS +ASH+++++Y +CIDCAFS+VAL+NS ++KN
Sbjct: 1087 SVLQLLSLCGRHPETKEQAVDALIGLMSFNASHLSQSSYAYCIDCAFSFVALRNSSVEKN 1146

Query: 1156 LKILDALSDSVNFLVQWYRNYCAESGNSFSVASN-ASSSSLDEKGLGSSNFALTLFLKLG 1215
            LKILD ++DSV  LV+WY+    ++ NS+S ASN +SSSS++E  L   NF   LFLKL 
Sbjct: 1147 LKILDLMADSVTMLVKWYKTASTDTANSYSPASNTSSSSSMEENNLRGVNFVHHLFLKLS 1206

Query: 1216 EALRKTSLARREEIRNHAITSLKKSFVLA-EELDFPPTNCIGCFNNIIFAMVDDLHEKML 1275
            EA RKT+LARREEIRN A+TSL+KSF +  E+L F P+ CI C +++IF  +DDLHEK+L
Sbjct: 1207 EAFRKTTLARREEIRNRAVTSLEKSFTMGHEDLGFTPSGCIYCIDHVIFPTIDDLHEKLL 1266

Query: 1276 EYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKA 1335
            +YSRR+NAERE RSM+GTLKI+M++L +V+LVYL+QI ES  FRTFWLGVLRRMDTCMKA
Sbjct: 1267 DYSRRENAEREMRSMEGTLKIAMKVLMNVFLVYLEQIVESAEFRTFWLGVLRRMDTCMKA 1326

Query: 1336 DLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDELFP 1387
            DLG YG++ L++++PELL  +I TM+EKEILV+KE +DLWEITYIQIQWIAP +KDELFP
Sbjct: 1327 DLGEYGDNKLQEVVPELLTTMIGTMKEKEILVQKEDDDLWEITYIQIQWIAPALKDELFP 1373

BLAST of Cucsat.G19767 vs. ExPASy Swiss-Prot
Match: Q42510 (ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=GN PE=1 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 3.1e-302
Identity = 589/1463 (40.26%), Postives = 894/1463 (61.11%), Query Frame = 0

Query: 4    RSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDSTIQ 63
            ++ ++E E            L+CM++TE+ ++LAV+RR        Y+S  D+   S I 
Sbjct: 11   KAIEEEPEDFECTDSSNTTTLACMIDTEIAAVLAVMRR-NVRWGGRYMSGDDQLEHSLI- 70

Query: 64   QSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFD 123
            QSLK+LR  +F   Q W TI P +Y+ P LDVI+SD+  A  T +ALS++ KI+ + + D
Sbjct: 71   QSLKALRKQVFSWNQPWHTISPMLYLQPFLDVIRSDETGAPITSIALSSVYKILNLNVID 130

Query: 124  EKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCT 183
            + T   +DA++L+V  +T+C+ E TD  +E+ V+MK+LQVL   M ++AS +L++Q VCT
Sbjct: 131  QNTANIEDAMHLVVDSVTSCRFEVTDPASEEVVLMKILQVLLACMKNKASVMLSNQHVCT 190

Query: 184  IVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIE------------------ 243
            +VNTCF VV Q+  +G+LLQR AR+TM+EL++ IFS LP++E                  
Sbjct: 191  VVNTCFRVVHQAGMKGELLQRVARHTMHELVRCIFSHLPDVERTETTLVNRAGSIKQEKA 250

Query: 244  ------------VRDGE-ESESDTE---------------DADLG-GS-----------L 303
                        V DG   SE D E               D  +G GS           +
Sbjct: 251  GVDSDYAIVSKPVEDGNANSEYDVENSMATFATGAQSLMDDGPVGPGSRKPASPYDLHIM 310

Query: 304  DSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRT----ADEDVQLFALVLINSAVE 363
               YG+  ++++FHFLCSLLNVVE +      G+GSR+     DEDV LFAL LINSA+E
Sbjct: 311  TEPYGVPSMVEIFHFLCSLLNVVEHV------GMGSRSNTIAFDEDVPLFALNLINSAIE 370

Query: 364  LSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLES 423
            L G +I  HP+LL ++QD+LF +L+ +G + +PL+LSM+CS VLN+Y  LR  ++LQLE+
Sbjct: 371  LGGSSIRHHPRLLSLIQDELFRNLMQFGLSMSPLILSMVCSIVLNLYQHLRTELKLQLEA 430

Query: 424  FFVYVALKLA--SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGK 483
            FF  V L+LA   +G S Q QEVA+E ++NFCRQ SF++E Y N DCD    N+ EE+  
Sbjct: 431  FFSCVILRLAQGKYGPSYQQQEVAMEALVNFCRQKSFMVEMYANLDCDITCSNVFEELSN 490

Query: 484  LLCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYI 543
            LL K +FP   PL+ ++I A +GL+ VI  +AE++         G   L + P  +DEY 
Sbjct: 491  LLSKSTFPVNCPLSAMHILALDGLIAVIQGMAERIS-------NGLTGLDLGPVHLDEYT 550

Query: 544  PFWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDP 603
            PFW  K     D   W+ +VR RK  K++++I   HFNRD KKGL +L+ + L+ D  DP
Sbjct: 551  PFWMVKCDNYSDPNHWVSFVRRRKYIKRRLMIGADHFNRDPKKGLEFLQGTHLLPDKLDP 610

Query: 604  KAYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRL 663
            ++ A FFRYT GLDK  VG++LG+  +F V+VL EF  TF+F  M LDTALR +LETFRL
Sbjct: 611  QSVACFFRYTAGLDKNLVGDFLGNHDEFCVQVLNEFAGTFDFQYMNLDTALRLFLETFRL 670

Query: 664  PGEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDE 723
            PGE+QKI R+LEAFSER+Y +QS    A+KD   VL YS+IMLNTDQHN QVKKKMTE++
Sbjct: 671  PGESQKIQRVLEAFSERYY-MQSPEILANKDAALVLSYSIIMLNTDQHNVQVKKKMTEED 730

Query: 724  FIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQL-DMNPSKWVELMNRSKIIQ 783
            FIRNNR IN G DLPR++LSELFHSI NN I  +P+ G    +M PS+W++LM++SK   
Sbjct: 731  FIRNNRHINGGNDLPREFLSELFHSICNNEIRTTPEQGAGFPEMTPSRWIDLMHKSKKTA 790

Query: 784  PFMSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQ-YGLE 843
            P++  D    L  DMF  ++GP++A+++  F+HA+ +++   CI+G  +IAKI+  + LE
Sbjct: 791  PYILADSRAYLDHDMFAIMSGPTIAAISVVFDHAEHEDVYQTCIDGFLAIAKISACHHLE 850

Query: 844  DTLDELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRN 903
            D LD+L+   CKFTTLLNP +S +E +  F  D K ++AT+ +FTIAN +GD IR GWRN
Sbjct: 851  DVLDDLVVSLCKFTTLLNP-SSVDEPVLAFGDDAKARMATITIFTIANKYGDYIRTGWRN 910

Query: 904  IVDCLLKLKRLKLLPQSVI-DFEVASTSSND------VARSDSGVIFPSQDPKFCTQQSS 963
            I+DC+L+L +L LLP  V  D    S  S++      +A S S     S       ++SS
Sbjct: 911  ILDCILRLHKLGLLPARVASDAADESEHSSEQGQGKPLANSLSSAHLQSMG---TPRRSS 970

Query: 964  GMVSRFSQFLSLDSME---DSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLG 1023
            G++ RFSQ LSLD+ E         L  +++ L+ I++C I +IF+ S  +  E+LL L 
Sbjct: 971  GLMGRFSQLLSLDTEEPRSQPTEQQLAAHQRTLQTIQKCHIDSIFTESKFLQAESLLQLA 1030

Query: 1024 RSLIFAAAGKGQKFSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFP 1083
            R+LI+ AAG+ QK ++  E+E+T  FC +L+I +T+ N  R  + W   +E++ T+ Q  
Sbjct: 1031 RALIW-AAGRPQKGTSSPEDEDTAVFCLELLIAITLNNRDRIVLLWQGVYEHIATIAQST 1090

Query: 1084 LFSAIPFAEKAVLGLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQ 1143
            +       +KA+ GL ++C RLL     + + +EL+ +S+ L+  LD  + D   E I  
Sbjct: 1091 VMPC-NLVDKAIFGLLRICQRLLP--YKESLADELL-RSLQLVLKLDARVADAYCEQIAI 1150

Query: 1144 SVSKILIEYPANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTF 1203
             VS+++     +++SQ GW+++  LLS T RHPE  + G + +  +MS+ +H+   NY  
Sbjct: 1151 EVSRLVKANANHIRSQAGWRTITSLLSITARHPEASESGFDAVSFVMSEGTHLYPANYVL 1210

Query: 1204 CIDCAFSYVALKNSPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLD 1263
            C+D A  +   +    +++++ LD + DS+ FL +W               + ++  ++ 
Sbjct: 1211 CVDAARQFAESRVGQSERSIRALDLMGDSLEFLAKW---------------ALSAKENMG 1270

Query: 1264 EKGLGSSNFAL-TLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIG 1323
            E+  G  +  +  ++L+L + LRK  L +RE++RNHA+ SL+K     + ++   +    
Sbjct: 1271 EEDFGKMSQDIGEMWLRLVQGLRKVCLDQREDVRNHALQSLQKCLGGVDGINLAHSMWSQ 1330

Query: 1324 CFNNIIFAMVDDLHEKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPG 1383
            CF+ +IF ++DDL    LE +    ++++ R+M+GTL ++++LL+ V+L  L+++S+   
Sbjct: 1331 CFDKVIFTVLDDL----LEIAA--GSQKDYRNMEGTLLLAIKLLSKVFLQQLQELSQLST 1390

Query: 1384 FRTFWLGVLRRMDTCMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKE---GEDL 1387
            F   WLGVL RM+  MK  +       L++ +PELL+ I+  M+ K +L+++    G+ L
Sbjct: 1391 FCKLWLGVLTRMEKYMKVKVRGKKSDKLQESVPELLKNILLVMKTKGVLLQRSALGGDSL 1427

BLAST of Cucsat.G19767 vs. ExPASy Swiss-Prot
Match: Q9FLY5 (ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=GNL1 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 1.6e-266
Identity = 539/1452 (37.12%), Postives = 848/1452 (58.40%), Query Frame = 0

Query: 17   SKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDSTIQQSLKSLRALIFHP 76
            SK  +  ++ M+N+E+G++LAV+RR        YI+  D+   S I  SLK LR  IF  
Sbjct: 22   SKPSKGAVASMINSEIGAVLAVMRR-NVRWGVRYIADDDQLEHSLI-HSLKELRKQIFSW 81

Query: 77   QQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLI 136
            Q  W+ +DP +YI P LDVI SD+  A  TGVALS++ KI+ +E+F  +T    +A+++I
Sbjct: 82   QSNWQYVDPRLYIQPFLDVILSDETGAPITGVALSSVYKILTLEVFTLETVNVGEAMHII 141

Query: 137  VIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSA 196
            V  + +C+ E TD  +E+ V+MK+LQVL   +  +AS  L++Q +CTIVNTC  VV QS+
Sbjct: 142  VDAVKSCRFEVTDPASEEVVLMKILQVLLACVKSKASNGLSNQDICTIVNTCLRVVHQSS 201

Query: 197  SRGDLLQRTARYTMNELIQIIFSRL----------------------------------- 256
            S+ +LLQR AR+TM+ELI+ IFS+L                                   
Sbjct: 202  SKSELLQRIARHTMHELIRCIFSQLPFISPLANECELHVDNKVGTVDWDPNSGEKRVENG 261

Query: 257  ----------------------PEIEVRDGEESESDTEDADLGGSLDSG----YGIRCVI 316
                                  PE ++R+ E+    ++D +   + ++     YGI C++
Sbjct: 262  NIASISDTLGTDKDDPSSEMVIPETDLRNDEKKTEVSDDLNAAANGENAMMAPYGIPCMV 321

Query: 317  DVFHFLCSLLNVVEIMVEVGDGGLGSRT----ADEDVQLFALVLINSAVELSGDAIGKHP 376
            ++FHFLC+LLNV E      +G + SR+     DEDV LFAL LINSA+EL G +  +HP
Sbjct: 322  EIFHFLCTLLNVGE------NGEVNSRSNPIAFDEDVPLFALGLINSAIELGGPSFREHP 381

Query: 377  KLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVYVALKLA 436
            KLL ++QDDLF +L+ +G + +PL+LS +CS VLN+Y  LR  +++QLE+FF YV L++A
Sbjct: 382  KLLTLIQDDLFCNLMQFGMSMSPLILSTVCSIVLNLYLNLRTELKVQLEAFFSYVLLRIA 441

Query: 437  --SFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLSFPTG 496
                G+S Q QEVA+E +++ CRQ +FI E + N+DCD    N+ E++  LL K +FP  
Sbjct: 442  QSKHGSSYQQQEVAMEALVDLCRQHTFIAEVFANFDCDITCSNVFEDVSNLLSKNAFPVN 501

Query: 497  SPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIPFWEEKSKED 556
             PL+ ++I A +GL+ ++  +AE++ +    +          P   + Y  FW  + +  
Sbjct: 502  GPLSAMHILALDGLISMVQGMAERVGEELPAS--------DVPTHEERYEEFWTVRCENY 561

Query: 557  LDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYFFRYT 616
             D   W+ +VR  K  KKK+++    FNRD  KGL YL+   L+ +  DPK+ A FFRYT
Sbjct: 562  GDPNFWVPFVRKVKHIKKKLMLGADRFNRDPNKGLQYLQGVHLLPEKLDPKSVACFFRYT 621

Query: 617  HGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRI 676
             GLDK  +G++LG+  QF ++VL EF +TF+F  M L TALR ++ TF+L GEAQKIHR+
Sbjct: 622  CGLDKNVMGDFLGNHDQFCIQVLHEFAKTFDFQNMNLATALRLFVGTFKLSGEAQKIHRV 681

Query: 677  LEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNREINA 736
            LEAFSER+YE QS +    KD  FVL YS+I+LNTDQHN QVK +MTE++FIRNNR IN 
Sbjct: 682  LEAFSERYYE-QSPHILIDKDAAFVLAYSIILLNTDQHNAQVKTRMTEEDFIRNNRTING 741

Query: 737  GKDLPRDYLSELFHSISNNAIILSPQ--SGLQLDMNPSKWVELMNRSKIIQPFMSYDFDP 796
            G DLPR+YLSE++HSI ++ I +     +G QL M  S+W+ ++ +SK   P++  D   
Sbjct: 742  GADLPREYLSEIYHSIRHSEIQMDEDKGTGFQL-MTASRWISVIYKSKETSPYIQCDAAS 801

Query: 797  RLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKIT-QYGLEDTLDELLAM 856
             L RDMF  ++GP++A+ +  FE A+++++L  CI+GL +IAK++  Y L   LD+L+  
Sbjct: 802  HLDRDMFYIVSGPTIAATSVVFEQAEQEDVLRRCIDGLLAIAKLSAYYHLNSVLDDLVVS 861

Query: 857  FCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLK 916
             CKFT    P  SA+E + V   D + ++AT AVF IAN +GD I  GW+NI++C+L L 
Sbjct: 862  LCKFTPFFAP-LSADEAVLVLGEDARARMATEAVFLIANKYGDYISAGWKNILECVLSLN 921

Query: 917  RLKLLPQSVI-----DFEVASTSSNDVARSDSGVIFPSQ-DPKFCTQQSSGMVSRFSQFL 976
            +L +LP  +      D E+++++      S + V   SQ  P    ++SS  + RF   L
Sbjct: 922  KLHILPDHIASDAADDPELSTSNLEQEKPSANPVPVVSQSQPSAMPRKSSSFIGRF--LL 981

Query: 977  SLDSMEDSLTLNLNE---YEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGK 1036
            S DS E     +  E   Y+     +K C I +IFS+S  +  E+L  L  SLI  A+GK
Sbjct: 982  SFDSEETKPLPSEEELAAYKHARGIVKDCHIDSIFSDSKFLQAESLQQLVNSLI-RASGK 1041

Query: 1037 GQKFSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEK 1096
                     +E +  FC +L+I +T+ N  R  + WP  +E++  +VQ  L +     EK
Sbjct: 1042 ---------DEASSVFCLELLIAVTLNNRDRILLIWPTVYEHILGIVQLTL-TPCTLVEK 1101

Query: 1097 AVLGLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYP 1156
            AV G+ K+C RLL     + + +EL+ KS+ L+  L  ++ D   E I Q V +++    
Sbjct: 1102 AVFGVLKICQRLLP--YKENLTDELL-KSLQLVLKLKAKVADAYCERIAQEVVRLVKANA 1161

Query: 1157 ANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVA 1216
            ++++S+ GW++++ LLS T RHPE  + G E L  +MS+ +H+  +NY  C+D A  +  
Sbjct: 1162 SHVRSRTGWRTIISLLSITARHPEASEAGFEALRFIMSEGAHLLPSNYELCLDAASHFAE 1221

Query: 1217 LKNSPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFA 1276
             +   +D+++  +D +S+SV  L +W +    E+ NS          S D   +G     
Sbjct: 1222 SRVGEVDRSISAIDLMSNSVFCLARWSQ----EAKNSIGETDAMMKLSED---IGK---- 1281

Query: 1277 LTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVD 1336
              ++LKL + L+K  L +R+E+RNHAI+ L+++   A+ +  P      CF++ +F ++D
Sbjct: 1282 --MWLKLVKNLKKVCLDQRDEVRNHAISMLQRAIAGADGIMLPQPLWFQCFDSAVFILLD 1341

Query: 1337 DLHEKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRR 1387
            D    +L +S  ++ +   ++++ TL ++ +L++  +L  L+ IS+ P F   W+GVL R
Sbjct: 1342 D----VLTFSIENSRKTLKKTVEETLVLATKLMSKAFLQSLQDISQQPSFCRLWVGVLNR 1401

BLAST of Cucsat.G19767 vs. ExPASy Swiss-Prot
Match: Q92538 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Homo sapiens OX=9606 GN=GBF1 PE=1 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 2.0e-91
Identity = 315/1275 (24.71%), Postives = 541/1275 (42.43%), Query Frame = 0

Query: 83   IDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVIGITN 142
            I+P++++ P L+VI+S+D     TG+AL+++ K +   + D    G  + +  +   +T+
Sbjct: 59   IEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTH 118

Query: 143  CKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLL 202
             +   TD  +++ V+MK+LQVL  L+       L ++SVC I+ +CF +  +     +LL
Sbjct: 119  ARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFE-MRLSELL 178

Query: 203  QRTARYTMNELIQIIFSRLPEI-------------------------------------- 262
            +++A +T+ +++Q++F+RLP+                                       
Sbjct: 179  RKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPP 238

Query: 263  ----EVRDGEE----------------------------SESDTEDADLGGSLDSG---- 322
                +V  G E                            + S+   A +  S DSG    
Sbjct: 239  RHMTKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFS 298

Query: 323  ------------------------------------------------------------ 382
                                                                        
Sbjct: 299  SQTTSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVL 358

Query: 383  -----------------------------------------YGIRCVIDVFHFLCSLLNV 442
                                                     YG+ C+ ++F FL SL N 
Sbjct: 359  EECTSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNP 418

Query: 443  -----VEIMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLF 502
                  E+M+ +G                 L L+  A+E +   + +   LL +++D++ 
Sbjct: 419  HDRHNSEVMIHMG-----------------LHLLTVALESA--PVAQCQTLLGLIKDEMC 478

Query: 503  HHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVYVALKLASFGNST---QI 562
             HL    +     + +        ++  +R  ++ Q+E  ++   +++ +  N     ++
Sbjct: 479  RHLFQLLSIERLNLYAASLRVCFLLFESMREHLKFQME-MYIKKLMEIITVENPKMPYEM 538

Query: 563  QEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLSFPTGSPLTTLNIQA 622
            +E+ALE I+   R  SF+ E Y+NYDCD    NL EE+ KLL K +FP    L T ++ +
Sbjct: 539  KEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLS 598

Query: 623  FEGLVIVI-----HNIAEKLD----KHKEETC---------------------------- 682
             + L+ VI     H  A+ L+    + K+ET                             
Sbjct: 599  LDALLTVIDSTEAHCQAKVLNSLTQQEKKETARPSCEIVDGTREASNTERTASDGKAVGM 658

Query: 683  ---------GGGGNL-----RVYPAQVDEYI------PFWEEKSKEDLDLEDWLRYVRVR 742
                      GGG L     +   + ++E +       F  +  +    L D    + ++
Sbjct: 659  ASDIPGLHLPGGGRLPPEHGKSGCSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIK 718

Query: 743  KAQKKKILIAG-HHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYFFRYTHGLDKQFVGEYL 802
               KKK+LI G   FN+  KKG+ +L+   L++ P D    A + R    LDK+ +GE++
Sbjct: 719  --NKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFV 778

Query: 803  GDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQ 862
             D  + ++ +L  F  TF F G+ LD ALR YLE FRLPGEA  I R+LEAF+ER+    
Sbjct: 779  SD--RKNIDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCN 838

Query: 863  SSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKK---MTEDEFIRNNREINAGKDLPRDYL 922
             S  FA+ D  F L Y++IMLNTDQHN  V+K+   MT +EF +N + +N GKD  +D L
Sbjct: 839  GS-PFANSDACFSLAYAVIMLNTDQHNHNVRKQNAPMTLEEFRKNLKGVNGGKDFEQDIL 898

Query: 923  SELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQ------PFMSYDFDPRLGRD 982
             +++H+I N  I++ P+    L      W  L++R    +      P  SYD       D
Sbjct: 899  EDMYHAIKNEEIVM-PEEQTGLVRENYVWNVLLHRGATPEGIFLRVPTASYDL------D 958

Query: 983  MFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKIT-QYGLEDTLDELLAMFCKFT 1042
            +F    GP++A+L+  F+ + E+ ++ + I G    A I+  YGL D  D L+   CKFT
Sbjct: 959  LFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFT 1018

Query: 1043 TLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLL 1098
             L +   S E    VF  + K  +A   VF +A+  GD +R GW+NI++ +L+L R +LL
Sbjct: 1019 ALSS--ESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLL 1078

BLAST of Cucsat.G19767 vs. ExPASy Swiss-Prot
Match: Q9R1D7 (Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Cricetulus griseus OX=10029 GN=GBF1 PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 2.2e-90
Identity = 310/1265 (24.51%), Postives = 534/1265 (42.21%), Query Frame = 0

Query: 83   IDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEKTPGAKDAINLIVIGITN 142
            I+P++++ P L+VI+S+D     TG+AL+++ K +   + D    G  + +  +   +T+
Sbjct: 59   IEPNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTH 118

Query: 143  CKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIVNTCFNVVQQSASRGDLL 202
             +   TD  +++ V+MK+LQVL  L+       L ++SVC I+ +CF +  +     +LL
Sbjct: 119  ARFVGTDPASDEVVLMKILQVLRTLLLTPVGTHLTNESVCEIMQSCFRICFE-MRLSELL 178

Query: 203  QRTARYTMNELIQIIFSRLPE--------------------------------------- 262
            +++A +T+ +++Q++F+RLP+                                       
Sbjct: 179  RKSAEHTLVDMVQLLFTRLPQFKEEPKSYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPP 238

Query: 263  --------------------------------------------------------IEVR 322
                                                                    +E+ 
Sbjct: 239  RHMTRVTPGSELPAPNGATLSCNLTSGMPFIDVPSSISSASSEAASAVVSPCTDSGLELS 298

Query: 323  DGEESESDTEDADLGGS------LDSG--------------------------------- 382
                S+ D  D +  GS       +SG                                 
Sbjct: 299  SQTTSKEDLTDLEQAGSPRESTTTESGSNEIGVSDQLDPQEGSHVEKAQSASVESIPEVL 358

Query: 383  ---------------------------------------YGIRCVIDVFHFLCSLLNVVE 442
                                                   YG+ C+ ++F FL SL N  +
Sbjct: 359  EECTSPPDHSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHD 418

Query: 443  IMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYG 502
                        R   E +    L L+  A+E +   + +   LL +++D++  HL    
Sbjct: 419  ------------RHNSEGMIHMGLHLLTVALESA--PVAQCQTLLGLIKDEMCRHLFQLL 478

Query: 503  AASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVYVALKLASFGNST---QIQEVALEG 562
            +     + +        ++  +R  ++ QLE  ++   +++ +  N     +++E+ALE 
Sbjct: 479  SVERLNLYAASLRVCFLLFESMREHLKFQLE-MYMKKLMEIITVENPKMPYEMKEMALEA 538

Query: 563  IINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLSFPTGSPLTTLNIQAFEGLVIV 622
            I+   R  SF+ E Y+NYDCD    NL E++ KLL K +FP    L T ++ + + L+ V
Sbjct: 539  IVQLWRIPSFVTELYINYDCDYYCANLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTV 598

Query: 623  I-----HNIAEKLD----KHKEET------------------------------------ 682
            I     H  A+ L+    + K+ET                                    
Sbjct: 599  IDSTEAHCQAKVLNTLTQQEKKETSRPSYEAVDSTQEANSTERATIDGKATGMASDALGL 658

Query: 683  -CGGGGNLRVYPAQ-----VDEY------IPFWEEKSKEDLDLEDWLRYVRVRKAQKKKI 742
                GG L     +     V+E         F  +  +    L D    + ++   KKK+
Sbjct: 659  HLQSGGWLSAEHGKPRCNDVEEAGDSGADKKFTRKPPRFSCLLPDPRELIEIK--NKKKL 718

Query: 743  LIAG-HHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYFFRYTHGLDKQFVGEYLGDPGQFH 802
            LI G   FN+  KKG+ +L+   L++ P D    A + R    LDK+ +GE++ D  + +
Sbjct: 719  LITGTEQFNQKPKKGIQFLQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSD--RKN 778

Query: 803  VKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQKIHRILEAFSERFYELQSSNTFAS 862
            + +L  F  TF F G+ LD ALR YLE FRLPGEA  IHR+LEAF+E +     S  FA+
Sbjct: 779  IDLLESFVSTFSFQGLRLDEALRLYLEAFRLPGEAPVIHRLLEAFTEHWRSCNGS-PFAN 838

Query: 863  KDTVFVLCYSLIMLNTDQHNPQVKKK---MTEDEFIRNNREINAGKDLPRDYLSELFHSI 922
             D  F L Y++IMLNTDQHN  V+K+   MT +EF +N + +N GKD  +D L +++H+I
Sbjct: 839  SDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAI 898

Query: 923  SNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQ------PFMSYDFDPRLGRDMFGCIAG 982
             N  I++ P+    L      W  L++R    +      P  SYD       D+F    G
Sbjct: 899  KNEEIVM-PEEQTGLVRENYVWSVLLHRGATPEGIFLRVPPGSYDL------DLFTMTWG 958

Query: 983  PSVASLAAFFEHADEDEMLNECIEGLFSIAKIT-QYGLEDTLDELLAMFCKFTTLLNPYA 1042
            P++A+L+  F+ + E+ ++ + I G    A I+  YGL D  D L+   CKFT L +   
Sbjct: 959  PTIAALSYVFDKSIEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSS--E 1018

Query: 1043 SAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKLKRLKLLPQSVIDF 1098
            S E    VF  + K  +A   VF +A+  GD +R GW+NI++ +L+L R +LLPQ++++ 
Sbjct: 1019 SIENLPTVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAVLQLFRAQLLPQAMVEV 1078

BLAST of Cucsat.G19767 vs. NCBI nr
Match: XP_004139429.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypothetical protein Csa_019390 [Cucumis sativus])

HSP 1 Score: 2737 bits (7096), Expect = 0.0
Identity = 1388/1388 (100.00%), Postives = 1388/1388 (100.00%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL
Sbjct: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL
Sbjct: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1388

BLAST of Cucsat.G19767 vs. NCBI nr
Match: KAA0061562.1 (ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710.1 ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa])

HSP 1 Score: 2683 bits (6955), Expect = 0.0
Identity = 1353/1388 (97.48%), Postives = 1375/1388 (99.06%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYI+TIDETYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE MVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVE-MVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            +F +YVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EEIGKL
Sbjct: 361  AFSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLS LVSDPPDP+
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPR 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 721  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTM NLYRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLST+QPDK+PEELIFKSINLMWMLDKEILD CFESITQSVSKILIEYPANL
Sbjct: 1021 GLFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSV SNASSSSLD+KGLGSSNFAL L
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALAL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKAD+GSYGESSLK+L+PELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1387

BLAST of Cucsat.G19767 vs. NCBI nr
Match: XP_008458277.1 (PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo])

HSP 1 Score: 2682 bits (6952), Expect = 0.0
Identity = 1352/1388 (97.41%), Postives = 1375/1388 (99.06%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYI+TIDETYDS
Sbjct: 3    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 63   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 123  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 183  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE MVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 243  LGGSMDSGYGIRCVIDVFHFLCSLLNVVE-MVEVGDGGLGSRTADEDVQLFALVLINSAV 302

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 303  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 362

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            +F +YVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EE+GKL
Sbjct: 363  AFSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKL 422

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 423  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 482

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLS LVSDPPDP+
Sbjct: 483  FWEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPR 542

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 543  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 602

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 603  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 662

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 663  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 722

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 723  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 782

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 783  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 842

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 843  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 902

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 903  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 962

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTM NLYRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 963  FSTPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1022

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLST+QPDK+PEELIFKSINLMWMLDKEILD CFESITQSVSKILIEYPANL
Sbjct: 1023 GLFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANL 1082

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1083 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1142

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSV SNASSSSLD+KGLGSSNFAL L
Sbjct: 1143 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALAL 1202

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1203 FLKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLH 1262

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1263 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1322

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKAD+GSYGESSLK+L+PELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1323 CMKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1382

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1383 ELFPEECF 1389

BLAST of Cucsat.G19767 vs. NCBI nr
Match: XP_038889761.1 (ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida])

HSP 1 Score: 2608 bits (6759), Expect = 0.0
Identity = 1312/1390 (94.39%), Postives = 1362/1390 (97.99%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            M+KRSSDDE+EKDSPKSKRRELGLSCMLNTEVG++LAVIRRPPS+LNSPYISTID+TYDS
Sbjct: 1    MEKRSSDDEEEKDSPKSKRRELGLSCMLNTEVGAVLAVIRRPPSDLNSPYISTIDDTYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPG KDAINLIV+GITNCKLEKTDLVTEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFNEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGE+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLG----SRTADEDVQLFALVLI 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVEI VE+GDGGLG    SRTADEDVQLFALVLI
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEI-VEIGDGGLGMGGGSRTADEDVQLFALVLI 300

Query: 301  NSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVR 360
            NSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGA+SNPLVLSMICSTVLNIYHFLRRFVR
Sbjct: 301  NSAVELSGDAIGKHPKLLRMVQDDLFHHLIHYGASSNPLVLSMICSTVLNIYHFLRRFVR 360

Query: 361  LQLESFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEE 420
            LQLE+FFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EE
Sbjct: 361  LQLEAFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEE 420

Query: 421  IGKLLCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVD 480
            IGKLLCKLSFPTGSPLTTLNIQAFEGLVI+IHNIAEKLDKHKEE+CGG G+LRVYPAQV 
Sbjct: 421  IGKLLCKLSFPTGSPLTTLNIQAFEGLVIMIHNIAEKLDKHKEESCGGNGSLRVYPAQVT 480

Query: 481  EYIPFWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDP 540
            EY PFWEEKSKEDL+LEDWL YVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLS LVSDP
Sbjct: 481  EYRPFWEEKSKEDLELEDWLNYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSQLVSDP 540

Query: 541  PDPKAYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLET 600
            PDPKAYA+FFRYTHGLDKQF+GEYLGDP QFHVKVLAEFT+TFEFTGMILDTALRTYLET
Sbjct: 541  PDPKAYAFFFRYTHGLDKQFIGEYLGDPDQFHVKVLAEFTDTFEFTGMILDTALRTYLET 600

Query: 601  FRLPGEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMT 660
            FRLPGEAQKIHRILEAFSERFY+LQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMT
Sbjct: 601  FRLPGEAQKIHRILEAFSERFYDLQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMT 660

Query: 661  EDEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKI 720
            EDEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKI
Sbjct: 661  EDEFIRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKI 720

Query: 721  IQPFMSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGL 780
            IQPFM  DFDPRLGRDMF CIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGL
Sbjct: 721  IQPFMLCDFDPRLGRDMFACIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGL 780

Query: 781  EDTLDELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWR 840
            EDTLDELLA+F KFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGD+IRGGW+
Sbjct: 781  EDTLDELLALFSKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDSIRGGWK 840

Query: 841  NIVDCLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRF 900
            NIVDCLLKLKRLKLLP SVIDFEVASTSSNDVARS+SGVIFPSQDPKFC+QQSSGM SRF
Sbjct: 841  NIVDCLLKLKRLKLLPPSVIDFEVASTSSNDVARSESGVIFPSQDPKFCSQQSSGMASRF 900

Query: 901  SQFLSLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAG 960
            SQFLSLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFS+SSNI DEAL +LGRSLIFAAAG
Sbjct: 901  SQFLSLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSSSSNIHDEALFHLGRSLIFAAAG 960

Query: 961  KGQKFSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAE 1020
            KGQKFSTP+EEEETVGFCWDLIITMTMANLYRFQVFWP+FHEYLQ VVQFPLFSAIPFAE
Sbjct: 961  KGQKFSTPVEEEETVGFCWDLIITMTMANLYRFQVFWPSFHEYLQAVVQFPLFSAIPFAE 1020

Query: 1021 KAVLGLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEY 1080
            KAVLGLFKVCLRLLSTYQP+K+PEELIFKSINLMWMLDKEILDTCFESIT+SVSKI+IEY
Sbjct: 1021 KAVLGLFKVCLRLLSTYQPEKLPEELIFKSINLMWMLDKEILDTCFESITKSVSKIIIEY 1080

Query: 1081 PANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYV 1140
            PANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSD +HITRTNYTFCIDCAFSYV
Sbjct: 1081 PANLQSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDGTHITRTNYTFCIDCAFSYV 1140

Query: 1141 ALKNSPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNF 1200
            ALKNSPL+KNLKILD+LSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLD+K LGSSNF
Sbjct: 1141 ALKNSPLEKNLKILDSLSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDDKSLGSSNF 1200

Query: 1201 ALTLFLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMV 1260
            +LTLFLKLGEALRKTSLARREEIRNHAI SLKKSFVLAEELDFPPTNCI CFNNIIFAMV
Sbjct: 1201 SLTLFLKLGEALRKTSLARREEIRNHAIASLKKSFVLAEELDFPPTNCISCFNNIIFAMV 1260

Query: 1261 DDLHEKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLR 1320
            DDLHEKMLEYSRRDNAEREARSM+GTLKISME+LTD+YL+YLKQISES GFRTFWLGVLR
Sbjct: 1261 DDLHEKMLEYSRRDNAEREARSMEGTLKISMEVLTDIYLLYLKQISESAGFRTFWLGVLR 1320

Query: 1321 RMDTCMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAP 1380
            RMDTCMKADLGSYGESSLK+L+P+LLRKIIT MREKEIL+KKEGEDLWEITYIQIQWIAP
Sbjct: 1321 RMDTCMKADLGSYGESSLKELVPDLLRKIITNMREKEILIKKEGEDLWEITYIQIQWIAP 1380

Query: 1381 GIKDELFPEE 1386
            GIKDELFPEE
Sbjct: 1381 GIKDELFPEE 1389

BLAST of Cucsat.G19767 vs. NCBI nr
Match: XP_022157565.1 (ARF guanine-nucleotide exchange factor GNL2 [Momordica charantia])

HSP 1 Score: 2466 bits (6391), Expect = 0.0
Identity = 1241/1383 (89.73%), Postives = 1321/1383 (95.52%), Query Frame = 0

Query: 6    SDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDSTIQQS 65
            SD+ED++DS KSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYIS ID+T+DS+IQQS
Sbjct: 5    SDEEDDRDSSKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYISPIDDTFDSSIQQS 64

Query: 66   LKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEK 125
            LKSLRALIFHPQQKWRTIDPSIYISPILDV+QSDDIPAAATGVALSALLKIIKVEIF+EK
Sbjct: 65   LKSLRALIFHPQQKWRTIDPSIYISPILDVVQSDDIPAAATGVALSALLKIIKVEIFNEK 124

Query: 126  TPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIV 185
            TPGAKDAINLIV+GITNCKLEKTDL+TEDAVMMK+LQVLAGLMNHRASYLLNDQSVCTIV
Sbjct: 125  TPGAKDAINLIVLGITNCKLEKTDLITEDAVMMKILQVLAGLMNHRASYLLNDQSVCTIV 184

Query: 186  NTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDADLGGSL 245
            NTCFNVVQQSA+RGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGE+SESDTEDADLGGSL
Sbjct: 185  NTCFNVVQQSANRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADLGGSL 244

Query: 246  DSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGD 305
            DSGYGIRCVIDVFHFLCSL+NVVE M E+ DG L SRTADEDVQLFALVLINSAVELSGD
Sbjct: 245  DSGYGIRCVIDVFHFLCSLMNVVE-MGEMMDG-LASRTADEDVQLFALVLINSAVELSGD 304

Query: 306  AIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVY 365
            AIGKHP+LLRMVQDDLFHHLIHYGA  NPLVLSMICSTVLNIYHFLRRFVRLQLE+FFVY
Sbjct: 305  AIGKHPRLLRMVQDDLFHHLIHYGARCNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFVY 364

Query: 366  VALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLS 425
            VAL+ AS+GNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNL E+IGKLLCKLS
Sbjct: 365  VALRSASYGNSTQIQEVALEGIINFCRQSSFILETYVNYDCDPLRWNLFEDIGKLLCKLS 424

Query: 426  FPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIPFWEEK 485
            FP GSPL++L IQAFEGLVI+IHNIAEKLDK  E     G +LRVYPA V EY PFWEE+
Sbjct: 425  FPAGSPLSSLQIQAFEGLVIMIHNIAEKLDK-LEGGPSRGSSLRVYPAHVSEYRPFWEER 484

Query: 486  SKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYF 545
            SKEDL+LE WL YVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLV DPPDPKAYA+F
Sbjct: 485  SKEDLELEGWLNYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVPDPPDPKAYAFF 544

Query: 546  FRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQK 605
            FRYT+GLDKQFVGEYLGDP  FHV+VLAEFT TFEFTGMILDTALRTYLETFRLPGEAQK
Sbjct: 545  FRYTYGLDKQFVGEYLGDPDPFHVRVLAEFTGTFEFTGMILDTALRTYLETFRLPGEAQK 604

Query: 606  IHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNR 665
            IHRILEAFSERFY+LQSSNTF +KD VFVLCYSLIMLNTDQHNPQVK+KMTEDEFIRNNR
Sbjct: 605  IHRILEAFSERFYDLQSSNTFVNKDAVFVLCYSLIMLNTDQHNPQVKRKMTEDEFIRNNR 664

Query: 666  EINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSYDF 725
            EIN GKDLPRDYLSELF SI+NNAIIL+PQSGLQLDMNPSKW+EL NRSKIIQPF+  DF
Sbjct: 665  EINGGKDLPRDYLSELFQSIANNAIILAPQSGLQLDMNPSKWIELTNRSKIIQPFVLCDF 724

Query: 726  DPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLDELLA 785
            D RLGRDMF CIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQ GLEDTLDELLA
Sbjct: 725  DIRLGRDMFACIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQCGLEDTLDELLA 784

Query: 786  MFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKL 845
            +F KFTTLLNPYASAEETLF FSHD+KPKLATLAVFTIANNFGD+IRGGWRNIVDCLLKL
Sbjct: 785  LFSKFTTLLNPYASAEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVDCLLKL 844

Query: 846  KRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLSLDSM 905
            KRLKLLPQSVI+FE+AST+ +D+A+S+SGVIFPSQDPKFCTQQSSGM SRFSQFLSLDSM
Sbjct: 845  KRLKLLPQSVIEFELASTAPHDLAKSESGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSM 904

Query: 906  EDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQKFSTPI 965
            EDSL+LNLNE+EQNLKFIKQCRIGNIFSNSSN+LD+ALLNLGRSLIFAAAGKGQKFSTPI
Sbjct: 905  EDSLSLNLNEFEQNLKFIKQCRIGNIFSNSSNLLDDALLNLGRSLIFAAAGKGQKFSTPI 964

Query: 966  EEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVLGLFKV 1025
            EEEETVGFCWDLIITM++AN++RFQVFWP+FHEYLQ V+QFPLFS IPFAEKAVLGLFKV
Sbjct: 965  EEEETVGFCWDLIITMSLANVHRFQVFWPSFHEYLQAVIQFPLFSTIPFAEKAVLGLFKV 1024

Query: 1026 CLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANLQSQIG 1085
            CL+LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFE+ITQSVSKILIEYPANLQS IG
Sbjct: 1025 CLKLLSTYQPEKYPEELIFKSINLMWMLDKEILDTCFEAITQSVSKILIEYPANLQSTIG 1084

Query: 1086 WKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSPLDK 1145
            WKSLLHLLSATGRHPETYDQGVETLIMLMSD +HITRTNYTFCIDCAFSYVALKNSPL+K
Sbjct: 1085 WKSLLHLLSATGRHPETYDQGVETLIMLMSDGTHITRTNYTFCIDCAFSYVALKNSPLEK 1144

Query: 1146 NLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTLFLKLG 1205
            NLKILD LSDSVNFL+QWYRNYCAESGNSFSV+SNASSSSL++KGLGSSNFALTLF+KLG
Sbjct: 1145 NLKILDLLSDSVNFLIQWYRNYCAESGNSFSVSSNASSSSLEDKGLGSSNFALTLFVKLG 1204

Query: 1206 EALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEKMLE 1265
            EALRKTSLARREEIRNHAI SLKKSF LAEELDF PTNCI CFN +IFAMVDDLHEKMLE
Sbjct: 1205 EALRKTSLARREEIRNHAIVSLKKSFFLAEELDFSPTNCINCFNLVIFAMVDDLHEKMLE 1264

Query: 1266 YSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKAD 1325
            YSRRDNAEREARSM+GTLKISM+LLTDVYL++LK ISES GFRTFWLGVLRRMDTCMKAD
Sbjct: 1265 YSRRDNAEREARSMEGTLKISMDLLTDVYLLFLKPISESAGFRTFWLGVLRRMDTCMKAD 1324

Query: 1326 LGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPE 1385
            LG+YGESSLKDL+P+LLRK+IT MREKEILV+KEG+DLWE TYIQIQWIAP IK+ELFPE
Sbjct: 1325 LGAYGESSLKDLVPDLLRKLITNMREKEILVQKEGDDLWETTYIQIQWIAPSIKEELFPE 1384

Query: 1386 ECF 1388
            E F
Sbjct: 1385 ESF 1384

BLAST of Cucsat.G19767 vs. ExPASy TrEMBL
Match: A0A0A0LF22 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 SV=1)

HSP 1 Score: 2737 bits (7096), Expect = 0.0
Identity = 1388/1388 (100.00%), Postives = 1388/1388 (100.00%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL
Sbjct: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL
Sbjct: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1388

BLAST of Cucsat.G19767 vs. ExPASy TrEMBL
Match: A0A5A7V061 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00190 PE=4 SV=1)

HSP 1 Score: 2683 bits (6955), Expect = 0.0
Identity = 1353/1388 (97.48%), Postives = 1375/1388 (99.06%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYI+TIDETYDS
Sbjct: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE MVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVE-MVEVGDGGLGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            +F +YVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EEIGKL
Sbjct: 361  AFSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLS LVSDPPDP+
Sbjct: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPR 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 721  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTM NLYRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLST+QPDK+PEELIFKSINLMWMLDKEILD CFESITQSVSKILIEYPANL
Sbjct: 1021 GLFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSV SNASSSSLD+KGLGSSNFAL L
Sbjct: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALAL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1261 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKAD+GSYGESSLK+L+PELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1321 CMKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1381 ELFPEECF 1387

BLAST of Cucsat.G19767 vs. ExPASy TrEMBL
Match: A0A1S3C8R1 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497742 PE=4 SV=1)

HSP 1 Score: 2682 bits (6952), Expect = 0.0
Identity = 1352/1388 (97.41%), Postives = 1375/1388 (99.06%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYI+TIDETYDS
Sbjct: 3    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYIATIDETYDS 62

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 63   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 122

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IFDEKTPG KDAINLIV+GITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS
Sbjct: 123  IFDEKTPGVKDAINLIVLGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 182

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+EVRDGEESESDTEDAD
Sbjct: 183  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEVRDGEESESDTEDAD 242

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE MVEVGDGGLGSRTADEDVQLFALVLINSAV
Sbjct: 243  LGGSMDSGYGIRCVIDVFHFLCSLLNVVE-MVEVGDGGLGSRTADEDVQLFALVLINSAV 302

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
            ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 303  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 362

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            +F +YVAL+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EE+GKL
Sbjct: 363  AFSIYVALRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEMGKL 422

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGG GNLRVYPAQVDEYIP
Sbjct: 423  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGSGNLRVYPAQVDEYIP 482

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSKEDLDLEDWLRYVRVRKAQKKKI IAGHHFNRDEKKGLAYLKLS LVSDPPDP+
Sbjct: 483  FWEEKSKEDLDLEDWLRYVRVRKAQKKKIFIAGHHFNRDEKKGLAYLKLSQLVSDPPDPR 542

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYAYFFRYTHGLDKQFVGEYLGDP QFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP
Sbjct: 543  AYAYFFRYTHGLDKQFVGEYLGDPDQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 602

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 603  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 662

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF
Sbjct: 663  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 722

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            MS DFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 723  MSCDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 782

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLAMFCKFTTLLNPYASAEETLFVFSHD+KPKLATLAVFTIANNFGDTIRGGWRNIVD
Sbjct: 783  DELLAMFCKFTTLLNPYASAEETLFVFSHDLKPKLATLAVFTIANNFGDTIRGGWRNIVD 842

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL
Sbjct: 843  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 902

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSLTLNLNEYEQNLKF+KQCRIGNIFSNS+NILDEALLNLGRSLIFAAAGKGQK
Sbjct: 903  SLDSMEDSLTLNLNEYEQNLKFVKQCRIGNIFSNSANILDEALLNLGRSLIFAAAGKGQK 962

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLIITMTM NLYRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 963  FSTPVEEEETVGFCWDLIITMTMTNLYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1022

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCLRLLST+QPDK+PEELIFKSINLMWMLDKEILD CFESITQSVSKILIEYPANL
Sbjct: 1023 GLFKVCLRLLSTHQPDKLPEELIFKSINLMWMLDKEILDNCFESITQSVSKILIEYPANL 1082

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN
Sbjct: 1083 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1142

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSV SNASSSSLD+KGLGSSNFAL L
Sbjct: 1143 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVVSNASSSSLDDKGLGSSNFALAL 1202

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            FLKLGEALRKTSLARREEIRNHAITSLKKSF+LAEELDFPPTNCIGCFNNIIFAMVDDLH
Sbjct: 1203 FLKLGEALRKTSLARREEIRNHAITSLKKSFLLAEELDFPPTNCIGCFNNIIFAMVDDLH 1262

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNAEREARSM+GTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT
Sbjct: 1263 EKMLEYSRRDNAEREARSMEGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1322

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKAD+GSYGESSLK+L+PELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD
Sbjct: 1323 CMKADIGSYGESSLKELVPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1382

Query: 1381 ELFPEECF 1388
            ELFPEECF
Sbjct: 1383 ELFPEECF 1389

BLAST of Cucsat.G19767 vs. ExPASy TrEMBL
Match: A0A6J1DTF7 (ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LOC111024228 PE=4 SV=1)

HSP 1 Score: 2466 bits (6391), Expect = 0.0
Identity = 1241/1383 (89.73%), Postives = 1321/1383 (95.52%), Query Frame = 0

Query: 6    SDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDSTIQQS 65
            SD+ED++DS KSKRRELGLSCMLNTEVG+ILAVIRRPPSELNSPYIS ID+T+DS+IQQS
Sbjct: 5    SDEEDDRDSSKSKRRELGLSCMLNTEVGAILAVIRRPPSELNSPYISPIDDTFDSSIQQS 64

Query: 66   LKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVEIFDEK 125
            LKSLRALIFHPQQKWRTIDPSIYISPILDV+QSDDIPAAATGVALSALLKIIKVEIF+EK
Sbjct: 65   LKSLRALIFHPQQKWRTIDPSIYISPILDVVQSDDIPAAATGVALSALLKIIKVEIFNEK 124

Query: 126  TPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQSVCTIV 185
            TPGAKDAINLIV+GITNCKLEKTDL+TEDAVMMK+LQVLAGLMNHRASYLLNDQSVCTIV
Sbjct: 125  TPGAKDAINLIVLGITNCKLEKTDLITEDAVMMKILQVLAGLMNHRASYLLNDQSVCTIV 184

Query: 186  NTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDADLGGSL 245
            NTCFNVVQQSA+RGDLLQRTARYTM+ELIQIIFSRLPEIEVRDGE+SESDTEDADLGGSL
Sbjct: 185  NTCFNVVQQSANRGDLLQRTARYTMHELIQIIFSRLPEIEVRDGEDSESDTEDADLGGSL 244

Query: 246  DSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAVELSGD 305
            DSGYGIRCVIDVFHFLCSL+NVVE M E+ DG L SRTADEDVQLFALVLINSAVELSGD
Sbjct: 245  DSGYGIRCVIDVFHFLCSLMNVVE-MGEMMDG-LASRTADEDVQLFALVLINSAVELSGD 304

Query: 306  AIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLESFFVY 365
            AIGKHP+LLRMVQDDLFHHLIHYGA  NPLVLSMICSTVLNIYHFLRRFVRLQLE+FFVY
Sbjct: 305  AIGKHPRLLRMVQDDLFHHLIHYGARCNPLVLSMICSTVLNIYHFLRRFVRLQLEAFFVY 364

Query: 366  VALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKLLCKLS 425
            VAL+ AS+GNSTQIQEVALEGIINFCRQSSFILE YVNYDCDPLRWNL E+IGKLLCKLS
Sbjct: 365  VALRSASYGNSTQIQEVALEGIINFCRQSSFILETYVNYDCDPLRWNLFEDIGKLLCKLS 424

Query: 426  FPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIPFWEEK 485
            FP GSPL++L IQAFEGLVI+IHNIAEKLDK  E     G +LRVYPA V EY PFWEE+
Sbjct: 425  FPAGSPLSSLQIQAFEGLVIMIHNIAEKLDK-LEGGPSRGSSLRVYPAHVSEYRPFWEER 484

Query: 486  SKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPKAYAYF 545
            SKEDL+LE WL YVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLV DPPDPKAYA+F
Sbjct: 485  SKEDLELEGWLNYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVPDPPDPKAYAFF 544

Query: 546  FRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLPGEAQK 605
            FRYT+GLDKQFVGEYLGDP  FHV+VLAEFT TFEFTGMILDTALRTYLETFRLPGEAQK
Sbjct: 545  FRYTYGLDKQFVGEYLGDPDPFHVRVLAEFTGTFEFTGMILDTALRTYLETFRLPGEAQK 604

Query: 606  IHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEFIRNNR 665
            IHRILEAFSERFY+LQSSNTF +KD VFVLCYSLIMLNTDQHNPQVK+KMTEDEFIRNNR
Sbjct: 605  IHRILEAFSERFYDLQSSNTFVNKDAVFVLCYSLIMLNTDQHNPQVKRKMTEDEFIRNNR 664

Query: 666  EINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPFMSYDF 725
            EIN GKDLPRDYLSELF SI+NNAIIL+PQSGLQLDMNPSKW+EL NRSKIIQPF+  DF
Sbjct: 665  EINGGKDLPRDYLSELFQSIANNAIILAPQSGLQLDMNPSKWIELTNRSKIIQPFVLCDF 724

Query: 726  DPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTLDELLA 785
            D RLGRDMF CIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQ GLEDTLDELLA
Sbjct: 725  DIRLGRDMFACIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQCGLEDTLDELLA 784

Query: 786  MFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVDCLLKL 845
            +F KFTTLLNPYASAEETLF FSHD+KPKLATLAVFTIANNFGD+IRGGWRNIVDCLLKL
Sbjct: 785  LFSKFTTLLNPYASAEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVDCLLKL 844

Query: 846  KRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFLSLDSM 905
            KRLKLLPQSVI+FE+AST+ +D+A+S+SGVIFPSQDPKFCTQQSSGM SRFSQFLSLDSM
Sbjct: 845  KRLKLLPQSVIEFELASTAPHDLAKSESGVIFPSQDPKFCTQQSSGMASRFSQFLSLDSM 904

Query: 906  EDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQKFSTPI 965
            EDSL+LNLNE+EQNLKFIKQCRIGNIFSNSSN+LD+ALLNLGRSLIFAAAGKGQKFSTPI
Sbjct: 905  EDSLSLNLNEFEQNLKFIKQCRIGNIFSNSSNLLDDALLNLGRSLIFAAAGKGQKFSTPI 964

Query: 966  EEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVLGLFKV 1025
            EEEETVGFCWDLIITM++AN++RFQVFWP+FHEYLQ V+QFPLFS IPFAEKAVLGLFKV
Sbjct: 965  EEEETVGFCWDLIITMSLANVHRFQVFWPSFHEYLQAVIQFPLFSTIPFAEKAVLGLFKV 1024

Query: 1026 CLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANLQSQIG 1085
            CL+LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFE+ITQSVSKILIEYPANLQS IG
Sbjct: 1025 CLKLLSTYQPEKYPEELIFKSINLMWMLDKEILDTCFEAITQSVSKILIEYPANLQSTIG 1084

Query: 1086 WKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKNSPLDK 1145
            WKSLLHLLSATGRHPETYDQGVETLIMLMSD +HITRTNYTFCIDCAFSYVALKNSPL+K
Sbjct: 1085 WKSLLHLLSATGRHPETYDQGVETLIMLMSDGTHITRTNYTFCIDCAFSYVALKNSPLEK 1144

Query: 1146 NLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTLFLKLG 1205
            NLKILD LSDSVNFL+QWYRNYCAESGNSFSV+SNASSSSL++KGLGSSNFALTLF+KLG
Sbjct: 1145 NLKILDLLSDSVNFLIQWYRNYCAESGNSFSVSSNASSSSLEDKGLGSSNFALTLFVKLG 1204

Query: 1206 EALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLHEKMLE 1265
            EALRKTSLARREEIRNHAI SLKKSF LAEELDF PTNCI CFN +IFAMVDDLHEKMLE
Sbjct: 1205 EALRKTSLARREEIRNHAIVSLKKSFFLAEELDFSPTNCINCFNLVIFAMVDDLHEKMLE 1264

Query: 1266 YSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDTCMKAD 1325
            YSRRDNAEREARSM+GTLKISM+LLTDVYL++LK ISES GFRTFWLGVLRRMDTCMKAD
Sbjct: 1265 YSRRDNAEREARSMEGTLKISMDLLTDVYLLFLKPISESAGFRTFWLGVLRRMDTCMKAD 1324

Query: 1326 LGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKDELFPE 1385
            LG+YGESSLKDL+P+LLRK+IT MREKEILV+KEG+DLWE TYIQIQWIAP IK+ELFPE
Sbjct: 1325 LGAYGESSLKDLVPDLLRKLITNMREKEILVQKEGDDLWETTYIQIQWIAPSIKEELFPE 1384

Query: 1386 ECF 1388
            E F
Sbjct: 1385 ESF 1384

BLAST of Cucsat.G19767 vs. ExPASy TrEMBL
Match: A0A6J1FBD6 (ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC111443895 PE=4 SV=1)

HSP 1 Score: 2459 bits (6372), Expect = 0.0
Identity = 1243/1388 (89.55%), Postives = 1322/1388 (95.24%), Query Frame = 0

Query: 1    MDKRSSDDEDEKDSPKSKRRELGLSCMLNTEVGSILAVIRRPPSELNSPYISTIDETYDS 60
            M+KRSS  +D K+S KSKRRELGLSCMLNTEVG++LAVIRRPPSELN+PYI T D+TYDS
Sbjct: 1    MEKRSSRHDDGKESSKSKRRELGLSCMLNTEVGAVLAVIRRPPSELNAPYICTADDTYDS 60

Query: 61   TIQQSLKSLRALIFHPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120
            +IQQSLKSLRALIF PQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE
Sbjct: 61   SIQQSLKSLRALIFQPQQKWRTIDPSIYISPILDVIQSDDIPAAATGVALSALLKIIKVE 120

Query: 121  IFDEKTPGAKDAINLIVIGITNCKLEKTDLVTEDAVMMKVLQVLAGLMNHRASYLLNDQS 180
            IF+EKTPGAKDAINLIV+GITNCKLEKTD+VTEDAVMMK+LQVLAGLMNHRASYLLNDQS
Sbjct: 121  IFEEKTPGAKDAINLIVLGITNCKLEKTDVVTEDAVMMKILQVLAGLMNHRASYLLNDQS 180

Query: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEIEVRDGEESESDTEDAD 240
            VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPE+E RD E+SESDTEDAD
Sbjct: 181  VCTIVNTCFNVVQQSASRGDLLQRTARYTMNELIQIIFSRLPEMEFRDEEDSESDTEDAD 240

Query: 241  LGGSLDSGYGIRCVIDVFHFLCSLLNVVEIMVEVGDGGLGSRTADEDVQLFALVLINSAV 300
            LGGS+DSGYGIRCVIDVFHFLCSLLNVVE   E+ DG LGSRTADEDVQLFALVLINSAV
Sbjct: 241  LGGSMDSGYGIRCVIDVFHFLCSLLNVVE-TAEMVDG-LGSRTADEDVQLFALVLINSAV 300

Query: 301  ELSGDAIGKHPKLLRMVQDDLFHHLIHYGAASNPLVLSMICSTVLNIYHFLRRFVRLQLE 360
             LSGDAIGKHPKLLRM+QDDLFHHLIHYGA SNPLVLSMICSTVLNIYHFLRRFVRLQLE
Sbjct: 301  GLSGDAIGKHPKLLRMIQDDLFHHLIHYGARSNPLVLSMICSTVLNIYHFLRRFVRLQLE 360

Query: 361  SFFVYVALKLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLLEEIGKL 420
            +FF+YV L+LASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNL EEIGKL
Sbjct: 361  AFFIYVVLRLASFGNSTQIQEVALEGIINFCRQSSFILEFYVNYDCDPLRWNLFEEIGKL 420

Query: 421  LCKLSFPTGSPLTTLNIQAFEGLVIVIHNIAEKLDKHKEETCGGGGNLRVYPAQVDEYIP 480
            LCKLSFP GSPLTTL IQAFEGLVI+IHNIAEKL+K +      GG+ R+YPAQV+ Y P
Sbjct: 421  LCKLSFPMGSPLTTLQIQAFEGLVIMIHNIAEKLEKEESS----GGSSRIYPAQVNVYRP 480

Query: 481  FWEEKSKEDLDLEDWLRYVRVRKAQKKKILIAGHHFNRDEKKGLAYLKLSLLVSDPPDPK 540
            FWEEKSK+DL  E+WL YVRVRKAQKKK+LIAGHHFNRDEKKGLAYLKL  LVSDPPDPK
Sbjct: 481  FWEEKSKDDLQYENWLDYVRVRKAQKKKVLIAGHHFNRDEKKGLAYLKLCQLVSDPPDPK 540

Query: 541  AYAYFFRYTHGLDKQFVGEYLGDPGQFHVKVLAEFTETFEFTGMILDTALRTYLETFRLP 600
            AYA+FFRYT+GLDKQ +GEYLGDP QFHV+VLAEFT+TFEFTGMILDTALRTYLETFRLP
Sbjct: 541  AYAFFFRYTNGLDKQLIGEYLGDPDQFHVRVLAEFTDTFEFTGMILDTALRTYLETFRLP 600

Query: 601  GEAQKIHRILEAFSERFYELQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660
            GEAQKIHRILEAFSERFY LQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF
Sbjct: 601  GEAQKIHRILEAFSERFYVLQSSNTFASKDTVFVLCYSLIMLNTDQHNPQVKKKMTEDEF 660

Query: 661  IRNNREINAGKDLPRDYLSELFHSISNNAIILSPQSGLQLDMNPSKWVELMNRSKIIQPF 720
            IRNNREINAGKDLPRDYLSELFHSISN+AIILSPQSGLQ DMNPSKWVELMNRSKIIQPF
Sbjct: 661  IRNNREINAGKDLPRDYLSELFHSISNHAIILSPQSGLQFDMNPSKWVELMNRSKIIQPF 720

Query: 721  MSYDFDPRLGRDMFGCIAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780
            M  DFDPRLGRDMF C+AGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL
Sbjct: 721  MLCDFDPRLGRDMFACVAGPSVASLAAFFEHADEDEMLNECIEGLFSIAKITQYGLEDTL 780

Query: 781  DELLAMFCKFTTLLNPYASAEETLFVFSHDMKPKLATLAVFTIANNFGDTIRGGWRNIVD 840
            DELLA+F K+TTLLNPYAS EETLF FSHD+KPKLATLAVFTIANNFGD+IRGGWRNIVD
Sbjct: 781  DELLAIFSKYTTLLNPYASTEETLFAFSHDLKPKLATLAVFTIANNFGDSIRGGWRNIVD 840

Query: 841  CLLKLKRLKLLPQSVIDFEVASTSSNDVARSDSGVIFPSQDPKFCTQQSSGMVSRFSQFL 900
            CLLKLKRLKLLPQSVIDFEVAST+SND+A+SDSGVIFPSQDPKF TQQSSGM  RFSQFL
Sbjct: 841  CLLKLKRLKLLPQSVIDFEVASTTSNDMAKSDSGVIFPSQDPKFGTQQSSGMAGRFSQFL 900

Query: 901  SLDSMEDSLTLNLNEYEQNLKFIKQCRIGNIFSNSSNILDEALLNLGRSLIFAAAGKGQK 960
            SLDSMEDSL+LNLNE+EQNLKFIKQCRIG+IFS+SS++ DEALLNLGRSLIFAAAGKGQK
Sbjct: 901  SLDSMEDSLSLNLNEFEQNLKFIKQCRIGSIFSSSSSLHDEALLNLGRSLIFAAAGKGQK 960

Query: 961  FSTPIEEEETVGFCWDLIITMTMANLYRFQVFWPNFHEYLQTVVQFPLFSAIPFAEKAVL 1020
            FSTP+EEEETVGFCWDLI TM++AN+YRFQVFWP+FHEYLQTVVQFPLFSAIPFAEKAVL
Sbjct: 961  FSTPVEEEETVGFCWDLITTMSLANVYRFQVFWPSFHEYLQTVVQFPLFSAIPFAEKAVL 1020

Query: 1021 GLFKVCLRLLSTYQPDKIPEELIFKSINLMWMLDKEILDTCFESITQSVSKILIEYPANL 1080
            GLFKVCL+LLSTYQP+K PEELIFKSINLMWMLDKEILDTCFESITQSVSKI+IEYPANL
Sbjct: 1021 GLFKVCLKLLSTYQPEKHPEELIFKSINLMWMLDKEILDTCFESITQSVSKIIIEYPANL 1080

Query: 1081 QSQIGWKSLLHLLSATGRHPETYDQGVETLIMLMSDASHITRTNYTFCIDCAFSYVALKN 1140
            Q+ IGWKSLLHLLSATGRHPETY+QGVETLIMLMSD +HITRTNYTFCI+CAFSYVALKN
Sbjct: 1081 QTAIGWKSLLHLLSATGRHPETYEQGVETLIMLMSDGTHITRTNYTFCIECAFSYVALKN 1140

Query: 1141 SPLDKNLKILDALSDSVNFLVQWYRNYCAESGNSFSVASNASSSSLDEKGLGSSNFALTL 1200
            SPLDKNLKILD LSDSVNFLVQWYRNYCAESGNS+SVASNASSSS ++KGLGS NFALTL
Sbjct: 1141 SPLDKNLKILDLLSDSVNFLVQWYRNYCAESGNSYSVASNASSSS-EDKGLGS-NFALTL 1200

Query: 1201 FLKLGEALRKTSLARREEIRNHAITSLKKSFVLAEELDFPPTNCIGCFNNIIFAMVDDLH 1260
            F+KLGEALRKTSLARREEIRNHA+ SLKKSF+LAEELDF  TNCI  FN ++FAMVDDLH
Sbjct: 1201 FIKLGEALRKTSLARREEIRNHAVISLKKSFMLAEELDFTSTNCINFFNLVVFAMVDDLH 1260

Query: 1261 EKMLEYSRRDNAEREARSMDGTLKISMELLTDVYLVYLKQISESPGFRTFWLGVLRRMDT 1320
            EKMLEYSRRDNA+REARSM+GTLKISM+LLTDVYL++LKQISES GFRTFWLG+LRRMDT
Sbjct: 1261 EKMLEYSRRDNAKREARSMEGTLKISMDLLTDVYLLFLKQISESSGFRTFWLGILRRMDT 1320

Query: 1321 CMKADLGSYGESSLKDLIPELLRKIITTMREKEILVKKEGEDLWEITYIQIQWIAPGIKD 1380
            CMKADLGSYGESSLKDL+PELLRKIIT MREKEIL+KKEG+DLWEITYIQIQWIAP IK+
Sbjct: 1321 CMKADLGSYGESSLKDLVPELLRKIITKMREKEILMKKEGDDLWEITYIQIQWIAPAIKE 1380

Query: 1381 ELFPEECF 1388
            ELFPEE F
Sbjct: 1381 ELFPEESF 1380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4K2K30.0e+0065.70ARF guanine-nucleotide exchange factor GNL2 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q425103.1e-30240.26ARF guanine-nucleotide exchange factor GNOM OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9FLY51.6e-26637.12ARF guanine-nucleotide exchange factor GNL1 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q925382.0e-9124.71Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Ho... [more]
Q9R1D72.2e-9024.51Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 OS=Cr... [more]
Match NameE-valueIdentityDescription
XP_004139429.10.0100.00ARF guanine-nucleotide exchange factor GNL2 [Cucumis sativus] >KGN60625.1 hypoth... [more]
KAA0061562.10.097.48ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo var. makuwa] >TYK10710... [more]
XP_008458277.10.097.41PREDICTED: ARF guanine-nucleotide exchange factor GNL2 [Cucumis melo][more]
XP_038889761.10.094.39ARF guanine-nucleotide exchange factor GNL2 [Benincasa hispida][more]
XP_022157565.10.089.73ARF guanine-nucleotide exchange factor GNL2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LF220.0100.00SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004740 PE=4 S... [more]
A0A5A7V0610.097.48ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C8R10.097.41ARF guanine-nucleotide exchange factor GNL2 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
A0A6J1DTF70.089.73ARF guanine-nucleotide exchange factor GNL2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1FBD60.089.55ARF guanine-nucleotide exchange factor GNL2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000904Sec7 domainSMARTSM00222sec7_5coord: 502..690
e-value: 1.9E-73
score: 260.0
IPR000904Sec7 domainPFAMPF01369Sec7coord: 506..690
e-value: 3.3E-65
score: 219.2
IPR000904Sec7 domainPROSITEPS50190SEC7coord: 503..688
score: 37.070827
IPR000904Sec7 domainCDDcd00171Sec7coord: 515..690
e-value: 1.01583E-73
score: 241.358
IPR032691Guanine nucleotide exchange factor, N-terminalPFAMPF12783Sec7_Ncoord: 251..410
e-value: 9.5E-26
score: 90.6
NoneNo IPR availableGENE3D1.10.220.20coord: 499..580
e-value: 2.1E-19
score: 71.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR10663:SF322ARF GUANINE-NUCLEOTIDE EXCHANGE FACTOR GNL2coord: 12..1386
NoneNo IPR availablePANTHERPTHR10663GUANYL-NUCLEOTIDE EXCHANGE FACTORcoord: 12..1386
IPR032629Mon2, dimerisation and cyclophilin-binding domainPFAMPF16213DCBcoord: 89..221
e-value: 3.4E-8
score: 33.4
IPR023394Sec7, C-terminal domain superfamilyGENE3D1.10.1000.11coord: 581..697
e-value: 5.8E-45
score: 154.4
IPR035999Sec7 domain superfamilySUPERFAMILY48425Sec7 domaincoord: 502..692
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 155..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G19767.T1Cucsat.G19767.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009846 pollen germination
biological_process GO:0015031 protein transport
biological_process GO:0032012 regulation of ARF protein signal transduction
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0005829 cytosol
cellular_component GO:0016020 membrane
cellular_component GO:0090406 pollen tube
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity