Cucsat.G19568 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G19568
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	kunitz trypsin inhibitor 1-like
Location	ctg4: 2556190 .. 2557176 (-)
RNA-Seq Expression	Cucsat.G19568
Synteny	Cucsat.G19568

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTCATATCCTCCATGTTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCATTTTTCAAACAAAACTAAATTACTCTTGTTGAACAATCAAAAGATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTATGTATTAAATCTGTTAGAGTGTTGATATACAACTATTAAATGTGTACCTACACTTCACTCACTATTTAAGCAGATAGATTATGATATATTGAATTCTTAACAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAATGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTCTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCCATCCTGAAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAACTAAATAAAACTTGATGTAAGGATTTACTTTTATGTGTGTTCATTGGGGTGTTATATATGATTAATATATATAATGTGATT

Coding sequence (CDS)

TTTTCATATCCTCCATGTTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCATTTTTCAAACAAAACTAAATTACTCTTGTTGAACAATCAAAAGATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAATGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTCTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCCATCCTGAAAGAGAAAAAATAA

Protein sequence

FSYPPCLAIKWHYTFASQNHHFSNKTKLLLLNNQKMMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK

Homology

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BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.3e-37
Identity = 84/193 (43.52%), Postives = 118/193 (61.14%), Query Frame = 0


Query: 49  WLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYV 108
           WL   +   S AQF    V+DT G P++ D +YFI+PAIT   G+LTL+TR  + CPF V
Sbjct: 15  WL--LVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNV 74

Query: 109 GQVPLLSQEIGFGVTLTPYLEG--DDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSG 168
           G  P   Q  GF V L+P++    +D +R GRDL+ +FQA T C   T+W++GE D  +G
Sbjct: 75  GLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTG 134

Query: 169 RRFVKLGNDNTATG----YFRIDKS-NLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVR 228
           RRF+  G D++  G    +FRI ++ + G++NI WCP++V    +  CG+ GI+ E G  
Sbjct: 135 RRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKI 194

Query: 229 FLALDGPAFPFEF 235
            LALDG A P  F
Sbjct: 195 LLALDGSALPVAF 197

[Close]

BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 4.5e-34
Identity = 79/205 (38.54%), Postives = 115/205 (56.10%), Query Frame = 0


Query: 37  MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 96
           MR     + + + +C F+   + AQF    VLDT G P++ D EYFI+P IT+  G  T+
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTM 62

Query: 97  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYL--EGDDTIREGRDLKFVFQAFTICITGT 156
           ++R  + CP +VG + L     G  V  TP+      D +R  RDL+  FQA + C+  T
Sbjct: 63  VSR-NESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQST 122

Query: 157 QWKVGEEDPKSGRRFVKLGNDNTATG----YFRIDKSNL-GVYNIGWCPSDVPFKGRPRC 216
           +W++GE+D KSGRR +  G D+   G    +FRI ++ L G+YNI WCP++V    +  C
Sbjct: 123 EWRLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFEC 182

Query: 217 GSAGILIEKGVRFLALDGPAFPFEF 235
           G+  +L E G   LALDG   P  F
Sbjct: 183 GTVDMLNENGKILLALDGGPLPLVF 201

[Close]

BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.8e-33
Identity = 79/197 (40.10%), Postives = 107/197 (54.31%), Query Frame = 0


Query: 44  IALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 103
           I + + +   MA  S AQF    V+DT G P++ D EYFI+PAIT   G  TL+T  G  
Sbjct: 8   IFILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-P 67

Query: 104 CPFYVGQVPLLSQEIGFGVTLTPYL--EGDDTIREGRDLKFVFQAFTICITGTQWKVGEE 163
           CP +VG +      +G  V  TP+     DD +R  RDL+  F   T C   T W++GE+
Sbjct: 68  CPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEK 127

Query: 164 DPKSGRRFVKLGNDNTA---TGYFRIDKSNL-GVYNIGWCPSDVPFKGRPRCGSAGILIE 223
           D  SGRR +  G DN A     +FRI ++   G YNI WCP++     + +CG+ G++ E
Sbjct: 128 DATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRE 187

Query: 224 KGVRFLALDGPAFPFEF 235
            G   LALDG A P  F
Sbjct: 188 NGKNLLALDGDALPVVF 198

[Close]

BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.1e-15
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0


Query: 67  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 126
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 127 YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 186
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 187 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 242
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

[Close]

BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 8.8e-14
Identity = 57/171 (33.33%), Postives = 76/171 (44.44%), Query Frame = 0


Query: 50  LCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVG 109
           L   +AI  +     PPV DTEG  L  D  Y++ PA     G LT+  R    CP  V 
Sbjct: 10  LLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCPLLVA 69

Query: 110 QVPLLSQEIGFGVTLTPY----LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKS 169
           Q     +  GF V  TP+       D TIR   D++  F A TIC+  T+W VG+E P +
Sbjct: 70  Q-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDE-PLT 129

Query: 170 GRRFVKLG-----NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGS 212
           G R V  G     + +     FR++K   G Y +  C       G  R G+
Sbjct: 130 GARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

[Close]

BLAST of Cucsat.G19568 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 451 bits (1161), Expect = 9.56e-160
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 95
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 96  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 155
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 156 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 215
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 216 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 251
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of Cucsat.G19568 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 416 bits (1068), Expect = 7.50e-146
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 54  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 113
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60

Query: 114 LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 173
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120

Query: 174 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 233
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180

Query: 234 FVRVDTGEELGSILKEKK 251
           FVRVDTGEELGSILKEKK
Sbjct: 181 FVRVDTGEELGSILKEKK 198

[Close]

BLAST of Cucsat.G19568 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 407 bits (1047), Expect = 2.36e-142
Identity = 197/217 (90.78%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 95
           MMRFHKSL A+FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 96  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 155
           TLITRKGDQCPFYVGQVPL SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 156 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 215
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 216 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 251
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of Cucsat.G19568 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 404 bits (1037), Expect = 7.59e-141
Identity = 195/216 (90.28%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 95
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 96  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 155
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 156 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 215
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 216 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 250
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of Cucsat.G19568 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 382 bits (982), Expect = 1.82e-132
Identity = 187/217 (86.18%), Postives = 199/217 (91.71%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 95
           MM FHKSL A+FS LCFFMAI S AQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMMFHKSL-AIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 96  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 155
           LITRKGDQCPFYVGQVPLLSQ+IGFGVT TPYL G+DTIREGRDLK VFQAFTICIT TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQ 120

Query: 156 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 215
           WKVGE DP +GRRFVKLGND+TATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180

Query: 216 IEKGVRFLALDGPAFPFEFVRVD-TGEELGSILKEKK 251
           IEKG+R LALDG AFPFEFVRV+  G++L SI +EKK
Sbjct: 181 IEKGLRLLALDGSAFPFEFVRVEIAGKKLDSIFQEKK 216

[Close]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 4.63e-160
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 95
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 96  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 155
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 156 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 215
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 216 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 251
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 407 bits (1047), Expect = 1.14e-142
Identity = 197/217 (90.78%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 95
           MMRFHKSL A+FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 96  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 155
           TLITRKGDQCPFYVGQVPL SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 156 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 215
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 216 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 251
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 404 bits (1037), Expect = 3.68e-141
Identity = 195/216 (90.28%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 36  MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 95
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 96  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 155
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 156 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 215
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 216 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 250
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 325 bits (833), Expect = 2.28e-110
Identity = 155/196 (79.08%), Postives = 172/196 (87.76%), Query Frame = 0


Query: 41  KSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRK 100
           KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRK
Sbjct: 5   KSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 64

Query: 101 GDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGE 160
           GDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE
Sbjct: 65  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 124

Query: 161 EDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGV 220
            DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+
Sbjct: 125 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 184

Query: 221 RFLALDGPAFPFEFVR 236
           R LALDG AFPFEFVR
Sbjct: 185 RLLALDGSAFPFEFVR 197

[Close]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 316 bits (809), Expect = 1.03e-106
Identity = 152/196 (77.55%), Postives = 168/196 (85.71%), Query Frame = 0


Query: 41  KSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRK 100
           KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL+TRK
Sbjct: 5   KSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 64

Query: 101 GDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGE 160
           GDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W+VGE
Sbjct: 65  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGE 124

Query: 161 EDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGV 220
            DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+
Sbjct: 125 ADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 184

Query: 221 RFLALDGPAFPFEFVR 236
           R LALDG AFPFEFVR
Sbjct: 185 RLLALDGSAFPFEFVR 197

The following BLAST results are available for this feature:

BLAST of Cucsat.G19568 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	3.3e-37	43.52	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	4.5e-34	38.54	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	3.8e-33	40.10	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
Q8RXD5	6.1e-15	30.60	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	8.8e-14	33.33	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Cucsat.G19568 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139193.1	9.56e-160	100.00	endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus]	[more]
KAE8651587.1	7.50e-146	100.00	hypothetical protein Csa_023465 [Cucumis sativus]	[more]
KAA0063688.1	2.36e-142	90.78	endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T...	[more]
XP_008455014.1	7.59e-141	90.28	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo]	[more]
XP_038888505.1	1.82e-132	86.18	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]

BLAST of Cucsat.G19568 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4	4.63e-160	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	1.14e-142	90.78	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C0M7	3.68e-141	90.28	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1FD70	2.28e-110	79.08	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	1.03e-106	77.55	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 66..238 e-value: 8.2E-27 score: 105.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 67..235 e-value: 1.6E-35 score: 122.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 43..237
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 67..83
None	No IPR available	GENE3D	2.80.10.50		coord: 64..239 e-value: 6.4E-38 score: 132.2
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 43..237
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 64..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G19568.T1	Cucsat.G19568.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity