Cucsat.G19240 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G19240
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	P-loop containing nucleoside triphosphate hydrolases superfamily protein
Location	ctg36: 373867 .. 376579 (+)
RNA-Seq Expression	Cucsat.G19240
Synteny	Cucsat.G19240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAGACATTTACTAAGGTTAGGTTTTGTTACTGTCTCTGATTCTTCTTCTTCTTCCTTTCCATTTCTTTCTTTTTGTTTTTTTGTCTCTGTATTAAAACTTATTTGTTGAGGAACACTAGTTTTGATTGAATTTTTTTAGGGTAGATTTTGTTTTCCCAAAGAGATGAACACCAGTCCATATTAAGCCATGAAATTCTTTGATTGTAAAATCTTCACCAAAATTTTTATAATTATTTTTGATGGTGTCATTCCAATTGTTTGTTAGTGCCGATTTCTTTGCAATCTTCAGAATTCTTTTCTTTGTTTTCAAGAATTTGTGTAGGTTGGTAGAGATTTGCACTTGTGAAACAACACAAGATTTTGTTATAGTTTAAATCTATAAAAATTAGAGAATCTAACAAGTGTGATTAACGATAACAATCTGAGGAGCTTGAGAGGGTAATTGCATCCAATGTGTGTGGATCATTATTTAACAAATATGAGGAGCTTTTGATGAGAAGAAATAATTGCATTGTAGTTTTGAAATCTGTTCAACAATCCATGGATTTGCTTGGTCTTCCACAAACTACAAATAAAAGAACCATTGTGAATTTCTATTTTCAGAATGCTTCATTGTTCTTTTGCACGGTTTCAGGTACGTTTAAGTTGTATTTGAAGTCATTAGAGCCATTGAAGGCTTTGGTGATTGATGAAGCAGCACAGTTGAAAGAGTGTGAGTGTGTTATTCCAATGCAACGGGCTGACATAAAGCATGCAATCCTCATAGGTGATGAGTGCCAATTGCCAGCATTGGTTTAGAGCAATGTAAGAAAGTTTCTCCCTTTATTTAATCATATTTGTTTTAACATATATTGACACTTCGATACACATTTGTTAAACATGATAAATACCTTTTGAACACTCACGTACACATATGGATAACCTAAACATTTCATAGACTTAAAATTCTCATACCCTATTTGAGAGGCTAAGCTCATTGGGGCATCCAAAACATCTTCTCAATGTGCAATAAAGAATGCATCCATCCATAAGCCTTTTCCCAAACTCAAAATTCTACTCAAGTCAGATCTCAGATGGTCCTAATGTCAAAGCTAAAGCCTATGAAAAGACGTTTCTTCCCGGACCGATGTTCGATTCGTATTCTTTCATAAACATAAATGGTGCACGAGAAGAAAAGGATGAAATTGGACACAGTTTGAAAAACATGGTAGAGGTTGATGTTGTGCTCAAAATTGTTCATTCTTTGTCTCAAGGTATAATATTTTCTCTATTTGATCTTTGTTTTCAGTTTGGTTACTTATGTGTCAGGTGAAATTTTCTCAAATTGTTATATACATTATTATTTTGCTATTTTTATGTAAATCTCTTTCGTTTTCTTAGAAGCATGAAAGCATTTCAACTTCGTTAGTGTGTCTTTCACATGTGTGTACCATACATATCGAATACTTTAGTTTTCCTACTGCGTTTGACCCTAAGATACTAAAGCCTAAATGTATAAGACCCTTGTTGGTGGAAAAGATTTTATCTCACAGTAGTTGTAGGAAATCAAAATAGTACTAAAATATGTTAGACATATATTTAATGCTTATTTCGTACTAAATAGACCGTAGATAAAATAGCAAAACTAGAGAGTGAAATACATGGTGTGTCAAAGTAAAATTTGAAATATATTGTTAAATTGCAGAACTCATAGACTATAATTTTTCATCTGTAAAACAGTATGTCTTCTAAAAACATGCATGTCCATGTTGTGCCCATGGCGTGTCATCTTGTTTCATGTCGAATCTTTAATACATATATATTTTTTAATATAATATATGTTTGTAGCATGTGTTGACTCAAAAGGAAAGATGAGCATTGGTGTAGTGTCCCCATATTCAGCTCAAGTCGTTACCATTCAACGCAAAATTGGGAAGAAGTACAATTGTAATGGATTTAATGTGAAGGTGAGTTCAGTTGATGGCTTTCAAGGAGGGGTGGAAGATATCATTATTATTTCCACTGTTTGGTGTAATACAGGATCGTCCATCGGGTTTTTGTCGAGTAATCAAAGAACAAATGTCGCACTTACAAGAGCTAGGTAGCTGCTTTCTAGTTTCTGTATGCTTACTCTTTTTTTGTTCCTTTACTTCAACATGTATAATTCAAATTAAATTAAACGCAGGGTGAGTTTCAAGTTGTTTATTTGGAGTGAAAGGAGCTTTTAGCTCATTAACTAAATGAAAACAATTTTCAAAATTAATCATCATATGATGTTTTTCTAATTTATGATTTTTTCCTTTCGATTGACCATTATAATGTATTTGAAAACTAGTCCTTTTCTTTGACTTAAGAGTAGGTACTGTTTGTGGATATTGGGAAACATCAAAACACTGTCAAAGAGTAACTCGGTTTGGGAAGAGTTGGTTATGGATGCCAAGAATCATGGCTGTTTCTTCAATGCTAATGCCAATGCCATAACCAAAGACATCCAACAGATAGTTCCACTACTGGATTATTTCAACTCCAATGATGAAGGAAGAAGCTTGGAGATAATGGAGGGAAAGAACGTAGAAGATCATGAATCCAAGGCCAAGAGGCTGCTGCAGTTGTTGTGGCAAGATTAACTTCATAAAAAATGGAGATGGAGGTATTCTTAAATGCTTATATCCCTAAAAATGACTTCAACACTTCTATTTATGAATTTCATGAGAATTTCATGAAGCCACTGGGCTGATTTTAAATTGCTAGGATGATCAACCTGCTGGGGAAGGTAACATTAATCTTGTTTTGTTTACATTTGAACTACTCAAATTTCTATCACATAAAAATTATCATTCATGAACATAATAAAGTGGATTTTAAAGATGTAGAAAATGACTTTTGTTTAGTGT

Coding sequence (CDS)

GAGACATTTACTAAGGTGATGAGTGCCAATTGCCAGCATTGGTTTAGAGCAATAATGCATCCATCCATAAGCCTTTTCCCAAACTCAAAATTCTACTCAAGTCAGATCTCAGATGGTCCTAATGTCAAAGCTAAAGCCTATGAAAAGACGTTTCTTCCCGGACCGATGTTCGATTCGTATTCTTTCATAAACATAAATGGTGCACGAGAAGAAAAGGATGAAATTGGACACAGTTTGAAAAACATGGTAGAGGTTGATGTTGTGCTCAAAATTGTTCATTCTTTGTCTCAAGCATGTGTTGACTCAAAAGGAAAGATGAGCATTGGTGTAGTGTCCCCATATTCAGCTCAAGTCGTTACCATTCAACGCAAAATTGGGAAGAAGTACAATTGTAATGGATTTAATGTGAAGGTGAGTTCAGTTGATGGCTTTCAAGGAGGGGTGGAAGATATCATTATTATTTCCACTGTTTGGTGTAATACAGGATCGTCCATCGGGTTTTTGTCGAGTAATCAAAGAACAAATGTCGCACTTACAAGAGCTAGGTACTGTTTGTGGATATTGGGAAACATCAAAACACTGTCAAAGAGTAACTCGGTTTGGGAAGAGTTGGTTATGGATGCCAAGAATCATGGCTGTTTCTTCAATGCTAATGCCAATGCCATAACCAAAGACATCCAACAGATAGTTCCACTACTGGATTATTTCAACTCCAATGATGAAGGAAGAAGCTTGGAGATAATGGAGGGAAAGAACGTAGAAGATCATGAATCCAAGGCCAAGAGGCTGCTGCAGTTGTTGTGGCAAGATTAA

Protein sequence

ETFTKVMSANCQHWFRAIMHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHSLKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSNSVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHESKAKRLLQLLWQD

Homology

[Close]

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot
Match: Q00416 (Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEN1 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 9.1e-27
Identity = 76/224 (33.93%), Postives = 127/224 (56.70%), Query Frame = 0


Query: 1    MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
            MHPSIS FP+S+FY  ++ DGP +            P+   Y F +I   R+E++    S
Sbjct: 1657 MHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPL-APYKFFDIISGRQEQNAKTMS 1716

Query: 61   LKNMVEVDVVLKIVHSLSQAC---VDSKGKMSIGVVSPYSAQVVTIQRKIGKKY-NCNGF 120
              NM E+ V +++V  L +     +D  GK  IG++SPY  Q+  ++++  + +      
Sbjct: 1717 YTNMEEIRVAIELVDYLFRKFDNKIDFTGK--IGIISPYREQMQKMRKEFARYFGGMINK 1776

Query: 121  NVKVSSVDGFQGGVEDIIIISTVWC-NTGSSIGFLSSNQRTNVALTRARYCLWILGNIKT 180
            ++  +++DGFQG  ++II+IS V   +T SS+GFL   +R NVALTRA+  +W+LG+ ++
Sbjct: 1777 SIDFNTIDGFQGQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLGHQRS 1836

Query: 181  LSKSNSVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFN 220
            L+KS  +W +L+ DAK+  C   A +  +     +   +L  FN
Sbjct: 1837 LAKS-KLWRDLIEDAKDRSCLAYACSGFLDPRNNRAQSILRKFN 1876

[Close]

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot
Match: O94387 (Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC29A10.10c PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.6e-26
Identity = 82/232 (35.34%), Postives = 124/232 (53.45%), Query Frame = 0


Query: 1    MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
            M+P IS FP+  FY+S++ DGPN+ A    + +   P    Y F N++G   E      S
Sbjct: 1585 MNPEISRFPSKFFYNSKLLDGPNMSA-VTSRPWHEDPQLGIYRFFNVHGT--EAFSNSKS 1644

Query: 61   LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGF-NVK 120
            L N+ E   +L +   L Q  ++   +  IGVV+PY +QV  ++ +  +KY    F ++ 
Sbjct: 1645 LYNVEEASFILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLD 1704

Query: 121  VSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKS 180
            + +VDGFQG  +DIII S V  +    IGFL   +R NVALTRA+  L+I+GN K L + 
Sbjct: 1705 IHTVDGFQGQEKDIIIFSCVRSSMSGGIGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQE 1764

Query: 181  NSVWEELVMDAKNHGCFFNANANAI--TKDIQQIVPLLDYFNSNDEGRSLEI 230
            + ++  L+ DAK  G + + +AN    +K I  +   L   N N   R   I
Sbjct: 1765 D-IFYSLIEDAKTRGVWRDLSANQFKNSKSISNVSTHLASNNLNLASRDTPI 1812

[Close]

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot
Match: Q92355 (Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.5e-25
Identity = 72/191 (37.70%), Postives = 108/191 (56.54%), Query Frame = 0


Query: 1    MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
            MHP IS FP+ KFY S++ DG N+  K  ++ +   P F  Y   ++ G  +E+     S
Sbjct: 1447 MHPDISHFPSKKFYDSRLEDGDNMAEKT-QQVWHVNPKFTQYRLFDVRG--KERTSNTMS 1506

Query: 61   LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFN-VK 120
              N+ EV+ ++ +V  L     D      IGV++PY +Q+  ++R    KY  +  + + 
Sbjct: 1507 TYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVITPYRSQLHELRRAFKVKYGKSFMSTID 1566

Query: 121  VSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKS 180
            + +VDGFQG  +DII  S V   +   IGFL   +R NVALTRAR  L I+GN++TL K+
Sbjct: 1567 IQTVDGFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARSSLLIIGNMETL-KT 1626

Query: 181  NSVWEELVMDA 191
            + +W  LV DA
Sbjct: 1627 DDLWGSLVDDA 1633

[Close]

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot
Match: B6SFA4 (Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.0e-22
Identity = 69/239 (28.87%), Postives = 122/239 (51.05%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDE-IGH 60
           MHP I  FP+ +FY   + DG +++A+   + +     F  + F +I+  +E +      
Sbjct: 558 MHPEIRSFPSKQFYEGALEDGSDIEAQT-TRDWHKYRCFGPFCFFDIHEGKESQHPGATG 617

Query: 61  SLKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFN-V 120
           S  N+ EV+ VL I H L     + K    + ++SPY+ QV T + +  + +       V
Sbjct: 618 SRVNLDEVEFVLLIYHRLVTMYPELKSSSQLAIISPYNYQVKTFKDRFKEMFGTEAEKVV 677

Query: 121 KVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSK 180
            +++VDGFQG  +D+ I S V  N    IGFLS+++R NV +TRA+  + ++G+  TL K
Sbjct: 678 DINTVDGFQGREKDVAIFSCVRANENGQIGFLSNSRRMNVGITRAKSSVLVVGSAATL-K 737

Query: 181 SNSVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVED 238
           S+ +W+ L+  A+     F  +            PL ++F S +   ++++ E   + D
Sbjct: 738 SDPLWKNLIESAEQRNRLFKVSK-----------PLNNFF-SEENLETMKLTEDMEIPD 782

[Close]

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot
Match: Q9FJR0 (Regulator of nonsense transcripts 1 homolog OS=Arabidopsis thaliana OX=3702 GN=UPF1 PE=1 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 3.9e-22
Identity = 74/214 (34.58%), Postives = 106/214 (49.53%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHP++S FP++ FY   + +G  +  +       P P+ +   F  +   +EE    G S
Sbjct: 724 MHPALSEFPSNSFYEGTLQNGVTIIERQTTGIDFPWPVPNRPMFFYVQLGQEEISASGTS 783

Query: 61  LKNMVEVDVVLKIVHS-LSQACVDSKGKMSIGVVSPYSAQ---VVTIQRKIGKKYNCNGF 120
             N  E   V K+V + L    V S+    IGV++PY  Q   +V    + G        
Sbjct: 784 YLNRTEAANVEKLVTAFLKSGVVPSQ----IGVITPYEGQRAYIVNYMARNGSLRQQLYK 843

Query: 121 NVKVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTL 180
            ++V+SVD FQG  +D II+S V  N    IGFL+  +R NVALTRARY + ILGN K L
Sbjct: 844 EIEVASVDSFQGREKDYIILSCVRSNEHQGIGFLNDPRRLNVALTRARYGIVILGNPKVL 903

Query: 181 SKSNSVWEELVMDAKNHGCFFNANANAITKDIQQ 211
           SK   +W  L+   K H C      N + + + Q
Sbjct: 904 SK-QPLWNGLLTHYKEHECLVEGPLNNLKQSMVQ 932

[Close]

BLAST of Cucsat.G19240 vs. NCBI nr
Match: XP_031737582.1 (uncharacterized ATP-dependent helicase C29A10.10c isoform X1 [Cucumis sativus])

HSP 1 Score: 509 bits (1310), Expect = 1.10e-181
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS
Sbjct: 13  MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 72

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 120
           LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV
Sbjct: 73  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 132

Query: 121 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN 180
           SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN
Sbjct: 133 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN 192

Query: 181 SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 240
           SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES
Sbjct: 193 SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 252

Query: 241 KAKRLLQLLWQD 252
           KAKRLLQLLWQD
Sbjct: 253 KAKRLLQLLWQD 264

[Close]

BLAST of Cucsat.G19240 vs. NCBI nr
Match: XP_031736106.1 (helicase sen1 isoform X3 [Cucumis sativus] >KAE8653666.1 hypothetical protein Csa_007501 [Cucumis sativus])

HSP 1 Score: 478 bits (1230), Expect = 1.93e-169
Identity = 240/252 (95.24%), Postives = 242/252 (96.03%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFYSSQISDGPNVK KAYEKTF+ GPMF SYSFININGAREEKDEIGHS
Sbjct: 13  MHPSISLFPNSKFYSSQISDGPNVKPKAYEKTFILGPMFGSYSFININGAREEKDEIGHS 72

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 120
           LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV
Sbjct: 73  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 132

Query: 121 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN 180
           SSVDGFQGG EDIIIISTV CNTGSSI FL SNQRTNVALTRARYCLW LGN KTLSKSN
Sbjct: 133 SSVDGFQGGEEDIIIISTVPCNTGSSIWFLLSNQRTNVALTRARYCLWTLGNFKTLSKSN 192

Query: 181 SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 240
           SVWEELVMDAKN GCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES
Sbjct: 193 SVWEELVMDAKNRGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 252

Query: 241 KAKRLLQLLWQD 252
           KAKRLLQLLW+D
Sbjct: 253 KAKRLLQLLWKD 264

[Close]

BLAST of Cucsat.G19240 vs. NCBI nr
Match: XP_031736102.1 (helicase sen1 isoform X2 [Cucumis sativus] >XP_031736103.1 helicase sen1 isoform X2 [Cucumis sativus] >XP_031736104.1 helicase sen1 isoform X2 [Cucumis sativus] >XP_031736105.1 helicase sen1 isoform X2 [Cucumis sativus])

HSP 1 Score: 472 bits (1215), Expect = 4.31e-167
Identity = 240/254 (94.49%), Postives = 241/254 (94.88%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFYSSQISDGPNVK KAYEKTF+ GPMF SYSFININGAREEKDEIGHS
Sbjct: 13  MHPSISLFPNSKFYSSQISDGPNVKPKAYEKTFILGPMFGSYSFININGAREEKDEIGHS 72

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 120
           LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV
Sbjct: 73  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 132

Query: 121 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN 180
           SSVDGFQGG EDIIIISTV CNTGSSI FL SNQRTNVALTRARYCLW LGN KTLSKSN
Sbjct: 133 SSVDGFQGGEEDIIIISTVPCNTGSSIWFLLSNQRTNVALTRARYCLWTLGNFKTLSKSN 192

Query: 181 SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 240
           SVWEELVMDAKN GCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES
Sbjct: 193 SVWEELVMDAKNRGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 252

Query: 241 KAKRLLQLL--WQD 252
           KAKRLLQLL  W D
Sbjct: 253 KAKRLLQLLCHWAD 266

[Close]

BLAST of Cucsat.G19240 vs. NCBI nr
Match: XP_031736100.1 (helicase sen1 isoform X1 [Cucumis sativus] >XP_031736101.1 helicase sen1 isoform X1 [Cucumis sativus])

HSP 1 Score: 471 bits (1213), Expect = 9.03e-167
Identity = 238/249 (95.58%), Postives = 239/249 (95.98%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFYSSQISDGPNVK KAYEKTF+ GPMF SYSFININGAREEKDEIGHS
Sbjct: 13  MHPSISLFPNSKFYSSQISDGPNVKPKAYEKTFILGPMFGSYSFININGAREEKDEIGHS 72

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 120
           LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV
Sbjct: 73  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 132

Query: 121 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKSN 180
           SSVDGFQGG EDIIIISTV CNTGSSI FL SNQRTNVALTRARYCLW LGN KTLSKSN
Sbjct: 133 SSVDGFQGGEEDIIIISTVPCNTGSSIWFLLSNQRTNVALTRARYCLWTLGNFKTLSKSN 192

Query: 181 SVWEELVMDAKNHGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 240
           SVWEELVMDAKN GCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES
Sbjct: 193 SVWEELVMDAKNRGCFFNANANAITKDIQQIVPLLDYFNSNDEGRSLEIMEGKNVEDHES 252

Query: 241 KAKRLLQLL 249
           KAKRLLQLL
Sbjct: 253 KAKRLLQLL 261

[Close]

BLAST of Cucsat.G19240 vs. NCBI nr
Match: XP_031737586.1 (probable helicase MAGATAMA 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 329 bits (843), Expect = 2.86e-111
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS
Sbjct: 13  MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 72

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 120
           LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV
Sbjct: 73  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYNCNGFNVKV 132

Query: 121 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRAR 164
           SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRAR
Sbjct: 133 SSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRAR 176

[Close]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL
Match: A0A6J1CTS8 (LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like OS=Momordica charantia OX=3673 GN=LOC111014153 PE=3 SV=1)

HSP 1 Score: 314 bits (805), Expect = 5.53e-99
Identity = 160/203 (78.82%), Postives = 175/203 (86.21%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEK-DEIGH 60
           MHPSISLFPNSKFYS+QISDGPNVKAK Y K+FLPGPMF SYSFI+IN  REEK D +GH
Sbjct: 381 MHPSISLFPNSKFYSNQISDGPNVKAKDYTKSFLPGPMFGSYSFIDINHGREEKRDGMGH 440

Query: 61  SLKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYN-CNGFNV 120
           S +NM EV +VLKI+H+LS+ACV SK K+SIGVVSPYSAQV  IQ K+G+KYN C GF V
Sbjct: 441 SWRNMAEVHIVLKILHTLSKACVRSKEKISIGVVSPYSAQVAAIQHKVGRKYNNCGGFTV 500

Query: 121 KVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSK 180
           KVSSVDGFQGG EDIIIISTV  N+GSSIGFLSSNQR NVALTRARYCLWILGN   LSK
Sbjct: 501 KVSSVDGFQGGEEDIIIISTVRSNSGSSIGFLSSNQRANVALTRARYCLWILGNFNALSK 560

Query: 181 SNSVWEELVMDAKNHGCFFNANA 201
           SNSV EELV+DAKN GCFF A+A
Sbjct: 561 SNSVXEELVIDAKNRGCFFRADA 583

[Close]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL
Match: A0A5D3CGE7 (Regulator of nonsense transcripts 1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5463G00100 PE=4 SV=1)

HSP 1 Score: 289 bits (739), Expect = 2.33e-92
Identity = 150/216 (69.44%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSIS FPNSKFYS+QISDGPNVK + Y K FL GPMF SYSF++IN  REEKD I  S
Sbjct: 181 MHPSISCFPNSKFYSNQISDGPNVKTEGYVKKFLNGPMFGSYSFMDINEGREEKDGITQS 240

Query: 61  LKNMVEVDVVLKIVHSL---SQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYN-CNGF 120
            KNM EVDVVL+I+H L   +  CVDS  K+SIGVVSPYSAQV  I+ K+G+ YN  N F
Sbjct: 241 WKNMAEVDVVLQIIHKLYNKATTCVDSNEKISIGVVSPYSAQVAAIEYKLGRNYNNSNIF 300

Query: 121 NVKVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTL 180
            V+VSSVDGFQGG EDIIIISTV  N  SSIGFLSSNQRTNVALTRARYCLWILGN  TL
Sbjct: 301 KVRVSSVDGFQGGEEDIIIISTVRSNRSSSIGFLSSNQRTNVALTRARYCLWILGNFDTL 360

Query: 181 SKSNSVWEELVMDAKNHGCFFNANANAITKDIQQIV 212
           S S+S+WE+LV DAKN GCFFNA  +   KD+  ++
Sbjct: 361 SNSDSIWEDLVFDAKNRGCFFNAKED---KDLANVM 393

[Close]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL
Match: A0A5B7AXI3 (Putative helicase MAGATAMA 3 (Fragment) OS=Davidia involucrata OX=16924 GN=Din_030858 PE=4 SV=1)

HSP 1 Score: 274 bits (701), Expect = 5.80e-90
Identity = 138/201 (68.66%), Postives = 163/201 (81.09%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSISLFPNSKFY +QI D PNV++K YE+ +LPGPMF  YSFINI G +EE  ++GHS
Sbjct: 19  MHPSISLFPNSKFYHNQILDAPNVQSKTYERHYLPGPMFGPYSFINIVGGKEELADVGHS 78

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKY-NCNGFNVK 120
            +N+VE+ VVLKIVH+L +A   SK K+SIGVVSPY+AQVVTIQ K+GKKY N + F VK
Sbjct: 79  RRNLVELAVVLKIVHNLYKAWNGSKKKLSIGVVSPYAAQVVTIQDKLGKKYENLDKFIVK 138

Query: 121 VSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKS 180
           V S+DGFQGG EDIIIISTV  NTG  +GFLSS QRTNVALTRAR+CLWILGN +TL+KS
Sbjct: 139 VKSIDGFQGGEEDIIIISTVRANTGGFVGFLSSPQRTNVALTRARHCLWILGNKRTLAKS 198

Query: 181 NSVWEELVMDAKNHGCFFNAN 200
            SVWE L+ DA+   C FNA+
Sbjct: 199 GSVWEALICDAERRQCLFNAD 219

[Close]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL
Match: A0A1S3CEY4 (uncharacterized protein LOC103500100 OS=Cucumis melo OX=3656 GN=LOC103500100 PE=4 SV=1)

HSP 1 Score: 291 bits (745), Expect = 5.82e-89
Identity = 150/216 (69.44%), Postives = 169/216 (78.24%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSIS FPNSKFYS+QISDGPNVK + Y K FL GPMF SYSF++IN  REEKD I  S
Sbjct: 616 MHPSISCFPNSKFYSNQISDGPNVKTEGYVKKFLNGPMFGSYSFMDINEGREEKDGITQS 675

Query: 61  LKNMVEVDVVLKIVHSL---SQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKYN-CNGF 120
            KNM EVDVVL+I+H L   +  CVDS  K+SIGVVSPYSAQV  I+ K+G+ YN  N F
Sbjct: 676 WKNMAEVDVVLQIIHKLYNKATTCVDSNEKISIGVVSPYSAQVAAIEHKLGRNYNNSNSF 735

Query: 121 NVKVSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTL 180
            V+VSSVDGFQGG EDIIIISTV  N  SSIGFLSSNQRTNVALTRARYCLWILGN  TL
Sbjct: 736 KVRVSSVDGFQGGEEDIIIISTVRSNRSSSIGFLSSNQRTNVALTRARYCLWILGNFDTL 795

Query: 181 SKSNSVWEELVMDAKNHGCFFNANANAITKDIQQIV 212
           S S+S+WE+LV DAKN GCFFNA  +   KD+  ++
Sbjct: 796 SNSDSIWEDLVFDAKNRGCFFNAKED---KDLANVM 828

[Close]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL
Match: A0A5C7HKA8 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_017989 PE=4 SV=1)

HSP 1 Score: 277 bits (708), Expect = 2.11e-83
Identity = 144/240 (60.00%), Postives = 179/240 (74.58%), Query Frame = 0


Query: 1   MHPSISLFPNSKFYSSQISDGPNVKAKAYEKTFLPGPMFDSYSFININGAREEKDEIGHS 60
           MHPSIS FPNS+FY +QI DGPNVK+++YE+ +LPGPMF  YSFINI G RE+  E+GHS
Sbjct: 640 MHPSISFFPNSEFYHNQILDGPNVKSQSYEQCYLPGPMFGPYSFINILGGREDHVEVGHS 699

Query: 61  LKNMVEVDVVLKIVHSLSQACVDSKGKMSIGVVSPYSAQVVTIQRKIGKKY-NCNGFNVK 120
             NMVEV VV+ I+ +L +A + SK K+SIGVVSPY AQVV IQ+++G+KY N +GF VK
Sbjct: 700 RSNMVEVSVVMNILQNLHKAWIRSKKKLSIGVVSPYKAQVVAIQKQLGRKYENRDGFVVK 759

Query: 121 VSSVDGFQGGVEDIIIISTVWCNTGSSIGFLSSNQRTNVALTRARYCLWILGNIKTLSKS 180
           V SVDGFQGG EDIIIISTV CN G  IGFLS  QR NVALTRARYCLWILGN +TL++S
Sbjct: 760 VKSVDGFQGGEEDIIIISTVRCNNGGYIGFLSKPQRVNVALTRARYCLWILGNERTLTRS 819

Query: 181 NSVWEELVMDAKNHGCFFNANANA-ITKDIQQIVPLLDYFNSND--EGRSLEIMEGKNVE 236
            SVW+ LV DAK+  CFFNA+ +  + + I ++    D F+     E  S E  + K++E
Sbjct: 820 ESVWQNLVRDAKDRRCFFNADDDKDLARAILEVKKEYDKFDEQGLLESCSYETKDNKSME 879

The following BLAST results are available for this feature:

BLAST of Cucsat.G19240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00416	9.1e-27	33.93	Helicase SEN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292...	[more]
O94387	2.6e-26	35.34	Uncharacterized ATP-dependent helicase C29A10.10c OS=Schizosaccharomyces pombe (...	[more]
Q92355	6.5e-25	37.70	Helicase sen1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 G...	[more]
B6SFA4	3.0e-22	28.87	Probable helicase MAGATAMA 3 OS=Arabidopsis thaliana OX=3702 GN=MAA3 PE=2 SV=1	[more]
Q9FJR0	3.9e-22	34.58	Regulator of nonsense transcripts 1 homolog OS=Arabidopsis thaliana OX=3702 GN=U...	[more]

BLAST of Cucsat.G19240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031737582.1	1.10e-181	100.00	uncharacterized ATP-dependent helicase C29A10.10c isoform X1 [Cucumis sativus]	[more]
XP_031736106.1	1.93e-169	95.24	helicase sen1 isoform X3 [Cucumis sativus] >KAE8653666.1 hypothetical protein Cs...	[more]
XP_031736102.1	4.31e-167	94.49	helicase sen1 isoform X2 [Cucumis sativus] >XP_031736103.1 helicase sen1 isoform...	[more]
XP_031736100.1	9.03e-167	95.58	helicase sen1 isoform X1 [Cucumis sativus] >XP_031736101.1 helicase sen1 isoform...	[more]
XP_031737586.1	2.86e-111	100.00	probable helicase MAGATAMA 3 isoform X2 [Cucumis sativus]	[more]

BLAST of Cucsat.G19240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTS8	5.53e-99	78.82	LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like OS=Momordica charantia OX=...	[more]
A0A5D3CGE7	2.33e-92	69.44	Regulator of nonsense transcripts 1-like protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A5B7AXI3	5.80e-90	68.66	Putative helicase MAGATAMA 3 (Fragment) OS=Davidia involucrata OX=16924 GN=Din_0...	[more]
A0A1S3CEY4	5.82e-89	69.44	uncharacterized protein LOC103500100 OS=Cucumis melo OX=3656 GN=LOC103500100 PE=...	[more]
A0A5C7HKA8	2.11e-83	60.00	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_017989 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 19..230 e-value: 2.4E-53 score: 182.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 19..208
IPR041679	DNA2/NAM7 helicase-like, C-terminal	PFAM	PF13087	AAA_12	coord: 19..191 e-value: 1.7E-44 score: 151.8
IPR041679	DNA2/NAM7 helicase-like, C-terminal	CDD	cd18808	SF1_C_Upf1	coord: 19..209 e-value: 7.34552E-54 score: 170.878
None	No IPR available	PANTHER	PTHR10887:SF474	OS04G0582700 PROTEIN	coord: 19..222
IPR045055	DNA2/NAM7-like helicase	PANTHER	PTHR10887	DNA2/NAM7 HELICASE FAMILY	coord: 19..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G19240.T1	Cucsat.G19240.T1	mRNA
Cucsat.G19240.T2	Cucsat.G19240.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007015	actin filament organization
biological_process	GO:0009860	pollen tube growth
biological_process	GO:0030050	vesicle transport along actin filament
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016459	myosin complex
cellular_component	GO:0031982	vesicle
molecular_function	GO:0051015	actin filament binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0000146	microfilament motor activity