Cucsat.G19232 (gene) Cucumber (B10) v3

Overview
NameCucsat.G19232
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionCBL-interacting serine/threonine-protein kinase 24
Locationctg36: 91988 .. 97558 (+)
RNA-Seq ExpressionCucsat.G19232
SyntenyCucsat.G19232
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATATTTGACGAGTTAACATTTTTTAAAAAGATGAAGGTATTTATTAGTGTTACTTTGCATAGTGTCATCAAATTTATTTTGGTTTCTTGTTCCTCTTTTGAGAATGCCCTCCTTCTTCTATCAAACCCATTATTTTCGATCATACTTCGGTTGCTTCCTTGTCTTCTCATTTCTTCCTCGCCCTTCATAGCCCCATTTTCCGCTTTTAACTGCTCCTTCTCCCGCGATTAACTCAATTCCAGTAAGTTTCTCACCCGGAATATCCCTTCTTTGCTCCTCCGATTCTTCTTCTTCCTGCTGCTCCTCACATTTTCCTTCTAATTACTAAACCAATTTTTTACGATTTATGCTTTCCTCTGCTCTCAGATTCTCCTGTCGGTTGTTTCTGATCAATTTTCCCTGCTACTCTTTCAAAATTTCGGTATCTATGCTGAAAAACAAGAAGAATATTAAGAAGATTGTGTTTCATGAAGAGAAATTGAGTGCGTAGCGGAATAAGGATTTGCACAAGGCTGAGCTGTATGATTCCAATTCAATTTCAAGCTCCGCCGTTCAATTAACGCTAATTTGGGTTATGGAATAGAATAATTGAGGATTTTGTTTTTTCTTATTTGATGACTCCTTGTTGAGTTGAGTCGATTGAGGTTTTTTTGTGCGTCATGTTGGATGGTTGTTCTTAGTTGAGAACTATCGAGGAGTTTGTTTTCTTCTGGGAAGTACGATCATGAAGCGACTTACTAGAAAAGTTGGGAAGTATGAGGTGGGGAGGACCGTTGGGGAAGGGACGTTTGCCAAGGTTAAGTTTGCATGTAACACGGAGACGGGGGAGGGTGTGGCCATGAAAGTATTGGCCAAAAGTAGCATTCTCAAGCACAAAATGGTACAACAGGTAGTTACTTACCTGAATTCTTCATAAATGTCTGGGAGGATTTAATGTCATAGTGCTGCCTAATGAGTATTATTGCCATGTTCATATAAAAATTCAGCATATGCACTTGTATTATTAATCCAAACTCGATGCTGTACCAATTGTATTATTAATCCGAGCTTGTTGGGCATCCCTTTTTTGTTCAACTGTACATCATGAAATCTATTTTAACCCCACTTTCCCTTAAGTGAATTAGAAACCGGATATATAACCTATTATGGAGGTAACGGTAAGACACATGGATTCACTACTCACTTGAAGATTCACATTGCCTATCACTCTATTAGGGAAGTCATGAGAATATACCAAGGATTATCTATATGAGGTGATGGCTTTTGCCATGGTTTAAGCTCAAACATGATCTCAGAGTTTTAGATACCCAGTATTTCCACCTGAATGAAAACTACACAGAAATCAAGAAATGAACAAACTCAGAGTTTTGAATTCAATACGCCTGCAACCTTCATGTCTGGAAGGATACTGCTATTGCTTATATGCAATAAAACTTGCCCTTTGCTTCATGTTATAACTATTTTGTTAATTTAATTTTTATTTTGTAAATTCTCTTTCAGATTAAGAGGGAGATATCAATAATGAAAATTGTCAGGCATCCACATATTGTCCGACTGCATGAGGTACTGCCATGGCTTTATGACAGTTTCAAATAACTTGTAGGATTTATGTTAATTAGTTTCCTGCTATTGGCATCTTTCATGATATCTCCCCCTAAATTTCAGGTGTTGGCTAGCCGGACAAAGATATATATTATTCTTGAGTTTGTCTCAGGAGGTGAACTATATGATAAAATAGTATGGCTTTCCTGCTTAAATAATTGAACACTATTATCACCTTCCTTGTATAAGATTGACGAGTATCAGAACTGTCTAATTATGATTATATATAATTGATCTGCCCAGGTTCTTCAAAAGAAGCTCCCAGAAATTGAGTCTAGACGATACTTTCATCAGCTGATAGATGCAGTTGCTCATTGTCACAGCAAGGGTGTGTACCACAGGGACCTTAAGGTGCAGAACTTTTAAATAGAGAATCTTGTTTAGATGTTCACAGTTATAGCAGAAAAAATGTTTCATATGTTCTGTTCCTAATTAATAACTTATTTCCTTGGTGTAGCCTGAAAACCTTCTTCTTGATGATGATGGAAATCTGAAAGTTTCCGACTTTGGACTGAGTGCACTGTCTCAAAAAGTATTGTGACAGTTATCTTGAATGATAGTAATTTTGTCAATAGTTATAACTTATATGCACATTACTCATTTCTCTAACCTATTCTGATTTGTTGCTGATCAGGGTGTTGACCTCCTTCACACGACATGTGGAACTCCTAATTATGTTGCACCCGAGGTACATTATCCATAAGCAATGTCAAAGTATAAGTACAAGTTAAAGACTCATGATTCGTGAGTTTTAAAGATTGTGCAGGATGTGAGAACATCCTTGCCCCAATGCATGTACTGTACATTGTTAAAATGCTCTTAAGTAGGTGGTTCCATTCATTTGGTCTGAATCATTGGACTTTTGTTTCTTTCTTCTAGGAATGTGTTCATGTTTACCTATCATTTTGTTTTTTAAATCATTATGTAAGCTGCTTCTTGCAGGTGCTTGGTGGTCAGGGTTATGATGGTGCAGCTGCAGATATATGGTCATGTGGAGTCATCCTCTATGTACTAATGGCTGGCTATCTTCCATTTTCAGAGATGGACCTTCCCACCCTAAACATGAAGGTCTGTTTCCAATCTTTACATCACCATAGTTTCCTCAGAATCGAATGACTAGCTCATGTCTATAATTAAGAATACCATTATGCCTGATACAAAAAATGCTTTTTAGGTTCCAACATCTACAACATTTATCCAATTAATGAAATATGGTGTTTTTTTTTTTTTAATCTTTTCTGTAATGATGTACTGGAGGTTGAGAAGCATGCACGGATGAACTGATTTTTGTATGCAATAGATGATGGTTCGACACTTTCACTACTGTAAATGAAAAGTTTCTAACAACTTCATTTCTGATGTATAGATAAATGCAGCAGAGTATTCCTGTCCCTATTGGTTTTCACCTGGTGCAAAGTCCTTGATACAGAAGATCCTTGATCCAAATCCTAAAGCTGTGAGTTTTTTTTTTTTTGTTTCAGAGATTTTGTTGCCGCTTCTCCAATTGTGAGATTTTTGGTTCTCTATAAATCAACGAATTCAATCATCATTAATAGTGTACTATTTTGGAATTTCATGTCTTGAACTGTAATTTGTATCTTGGGTAGGGTTTTTGGTTTTAATGATGTATCATTATTCTGCCGAATGTTGGAAACATTTGTCATGTTGGGTGAGTTCACGTGAAAGTTTGGCATTTATCATTCTTTGTGGAGTACTTAAATAAAGAAAAAGCAGCCACCATAGCATGTGGGATTTAGTATCTGTGTATTGTTTAAAAATTTCACCAGAGGTTCTCATTTATGTCCTGCAGCGAATTCGAATTGAAGAAATTAGAAAAGATCCATGGTTCAGAAGAAACTACATCCCTGCTAAACTTGGAGAAGATGAGGAAGTAAACTTAGATGATGTTCGGGCTGTTTTTGATGACATTGAGGTTGGTTTTTGTTTCCTTTATAGTTTAGGATTTTTCATCTCTCCAAAATGAAAACATTTAGGGATAATATACAACAATGAGCACTACTTGAACCTGCCAGAACCCACCCTTCCTTATAGGAGAGAGCAGTTTGATATAAAACAAATGGCCAAACTATATGTTAGGTACTAGATATTTAGTATGATGTTAGTTAAGGGGTATAAGAGAAAATCAGATAGATAGGAAGTTACTGTAGTTATCGTGTAAAGGTGATTACTAGTAGTTACTGTGTAAGTTTGTTTACCACTTGTTATAAATAGAGGGCGGGTAAGTGAGAGTAAAGGAGGTTATTCTGTGCAGTGATCTAAGGCTTGGGTGAGAGTACTCAAGAGGGAGGTTCCAAGTGGCTTATATTTGGGTTTATCTTGTATTTCTTACAGTTACATTATAATAAATTTAGATCTTGATCTTGTTATGAAGTATGCTAATGCTATACAGTAATGTTTAAATTGTTTTTTTGGATCTTTTTAATCATTGCATATAACCACATTTTTTTTATGGAGCATACTATACTGAATTTTCTGTGCAACGAAATGCTTTCTTCCTTTTTCTAATTGCTTAGCTTGTGTTACTTGACCTTGTTAATATTTACATGTTTTTCATGCATATTTGTGCACTAAATTATTTCTTTTCACTCACATCAGGACTTGCCATTTTGCAGGATGAATATGTAGCCGATCAAACAGAAAAAAGTGATGATGGTCCTTTGATAATGAATGCATTTGAGATGATAACATTATCTCAAGGATTAAATCTGTCACCACTATTTGATCGGCGACAGGTTAGATCATGTCAAGCTTTTTTTTTATTTTTTTTCCTTCTTTTTCTTCTTCATTTTTGGCATAGAATGTTGAGCTCCATAGTATTTTCCTCTCATGCCATAATGTGACCATCTCGTATTTTATACCTACTTGTTGGAACTTTGACATTGACCAATTTTCTCATCTCATTCTTTTCTCTTGAAAAATTTTCCATGACAGGACTATATAAAGCGGCAAACACGTTTTGTTTCTCGTAAACCTGCTAGCTTTATAATGTCTAGCATTGAAGCAGTAGCAGAAGCAATGGGATTAAATGTCCAGACACGTAATTTCAAGGTACGCTTTTATTCTTCCCTCATGACGAATAAAGTGTAGTACTATGATATAGTTTTGGAATTAAATATTAATTTCAATTTCAATTTTTCTTCACAGACAAGGTTGGAAAGAAGGACCGGATACAGGGTTGCACAGTTTGCCGCTGTGCTGGAGGTAATCTCACAGACATGATGATAGATAATCCAAGGTTTTATAAATTTCTTAGAAACTTGTTATTTCTGGGTTTTAGATTTATGAGGTGGCACCATCCCTTTTTATGGTGGATGTCCGCAAAGCTGCCGGAGATACTCTAGAATATCACAAGGTAATTTCATACAATTAGACATCGATGCTCAAAAAGATATAGGGAGGTTTAAATCCTACGTATACACTTCACTTTTTTGTTTGTGTGACCTTGTAGTTCTACAAGAATTTCTGTGGTAAGCTAGAAAACATAATCTGGAAACCATCTGAGAGTGTGGGAAACTGAAGCCCTGCTTGGTTACTAACTGATTCCATGGAGCAAATGAACAAACACACTTAATCAAAGATGCAGCAGAGGATCATGAAGGTTCACTTTCTCTATGATCTGAATTTTGTATATGCTGTTATGATATAACTCAGGAAGTGTGGAATTCTCATCATTCCTGCGTTTAAAGGTTCCGTCACAGGAGAAGAAAAAGAATAAGAAAAGAACTGGTTGAGTGTGAGAAAGTGAAAATGCATCCTTAGGAAATTTGCCTTGGATTGTACAGAATGTGATTGAAAGTAAATTTAGAATCTTTTGCTGTGAGCTGTTGAACGTAAATTTGTTAATAACTAACTAATAGTCCTTTATAAACTATATTTTCTTTACATGTCTCAAATAATCCTTTTTCCAATACTATAAAGAAAATTTAATAAAGGAATATTTTGATTTT

Coding sequence (CDS)

ATGAGGTGTTGGCTAGCCGGACAAAGATATATATTATTCTTGAGTTTGTCTCAGGAGGTTCTTCAAAAGAAGCTCCCAGAAATTGAGTCTAGACGATACTTTCATCAGCTGATAGATGCAGTTGCTCATTGTCACAGCAAGGGTGTGTACCACAGGGACCTTAAGCCTGAAAACCTTCTTCTTGATGATGATGGAAATCTGAAAGTTTCCGACTTTGGACTGAGTGCACTGTCTCAAAAAGGTGTTGACCTCCTTCACACGACATGTGGAACTCCTAATTATGTTGCACCCGAGGTGCTTGGTGGTCAGGGTTATGATGGTGCAGCTGCAGATATATGGTCATGTGGAGTCATCCTCTATGTACTAATGGCTGGCTATCTTCCATTTTCAGAGATGGACCTTCCCACCCTAAACATGAAGATAAATGCAGCAGAGTATTCCTGTCCCTATTGGTTTTCACCTGGTGCAAAGTCCTTGATACAGAAGATCCTTGATCCAAATCCTAAAGCTCGAATTCGAATTGAAGAAATTAGAAAAGATCCATGGTTCAGAAGAAACTACATCCCTGCTAAACTTGGAGAAGATGAGGAAGTAAACTTAGATGATGTTCGGGCTGTTTTTGATGACATTGAGGATGAATATGTAGCCGATCAAACAGAAAAAAGTGATGATGGTCCTTTGATAATGAATGCATTTGAGATGATAACATTATCTCAAGGATTAAATCTGTCACCACTATTTGATCGGCGACAGGACTATATAAAGCGGCAAACACGTTTTGTTTCTCGTAAACCTGCTAGCTTTATAATGTCTAGCATTGAAGCAGTAGCAGAAGCAATGGGATTAAATGTCCAGACACGTAATTTCAAGACAAGGTTGGAAAGAAGGACCGGATACAGGGTTGCACAGTTTGCCGCTGTGCTGGAGATTTATGAGGTGGCACCATCCCTTTTTATGGTGGATGTCCGCAAAGCTGCCGGAGATACTCTAGAATATCACAAGTTCTACAAGAATTTCTGTGGTAAGCTAGAAAACATAATCTGGAAACCATCTGAGAGTGTGGGAAACTGA

Protein sequence

MRCWLAGQRYILFLSLSQEVLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQKGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN
Homology
BLAST of Cucsat.G19232 vs. ExPASy Swiss-Prot
Match: Q9LDI3 (CBL-interacting serine/threonine-protein kinase 24 OS=Arabidopsis thaliana OX=3702 GN=CIPK24 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-143
Identity = 242/331 (73.11%), Postives = 280/331 (84.59%), Query Frame = 0

Query: 24  KLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQKGVD 83
           +L E ESR+YF QL+DAVAHCH KGVYHRDLKPENLLLD +GNLKVSDFGLSAL Q+GV+
Sbjct: 105 RLEESESRKYFQQLVDAVAHCHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVE 164

Query: 84  LLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKINA 143
           LL TTCGTPNYVAPEVL GQGYDG+AADIWSCGVIL+V++AGYLPFSE DLP L  KINA
Sbjct: 165 LLRTTCGTPNYVAPEVLSGQGYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINA 224

Query: 144 AEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLDDV 203
           AE+SCP WFS   K LI +ILDPNPK RI+I+ I+KDPWFR NY+P +  E+EEVNLDD+
Sbjct: 225 AEFSCPPWFSAEVKFLIHRILDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDI 284

Query: 204 RAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFVSR 263
           RAVFD IE  YVA+  E++D+GPL+MNAFEMITLSQGLNLS LFDRRQD++KRQTRFVSR
Sbjct: 285 RAVFDGIEGSYVAENVERNDEGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSR 344

Query: 264 KPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVDVR 323
           +  S I+++IEAVA +MG    TRNFKTRLE  +  +  Q A V+EIYEVAPSLFMVDVR
Sbjct: 345 REPSEIIANIEAVANSMGFKSHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVR 404

Query: 324 KAAGDTLEYHKFYKNFCGKLENIIWKPSESV 355
           KAAG+TLEYHKFYK  C KLENIIW+ +E +
Sbjct: 405 KAAGETLEYHKFYKKLCSKLENIIWRATEGI 435

BLAST of Cucsat.G19232 vs. ExPASy Swiss-Prot
Match: Q69Q47 (CBL-interacting protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK24 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-141
Identity = 237/331 (71.60%), Postives = 281/331 (84.89%), Query Frame = 0

Query: 22  QKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQKG 81
           Q KL E E+R+YF QLIDA+ +CHSKGVYHRDLKPENLLLD  GNLKVSDFGLS L+QKG
Sbjct: 110 QGKLRENEARKYFQQLIDAINYCHSKGVYHRDLKPENLLLDSRGNLKVSDFGLSTLAQKG 169

Query: 82  VDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKI 141
           V LLHTTCGTPNYVAPEVL   GYDG+AAD+WSCGVILYVLMAGYLPF E DLPTL  KI
Sbjct: 170 VGLLHTTCGTPNYVAPEVLSNNGYDGSAADVWSCGVILYVLMAGYLPFEEDDLPTLYDKI 229

Query: 142 NAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLD 201
            A ++SCPYWFSPGA SLI +ILDPNPK RI IE+IR+D WF++ Y+  K GEDE V+LD
Sbjct: 230 TAGQFSCPYWFSPGATSLIHRILDPNPKTRITIEQIREDTWFKKTYVAIKRGEDENVDLD 289

Query: 202 DVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFV 261
           DV+AVFD+IED+YV++Q   +D GPL+MNAFEMITLSQGL+LS LFDR+Q+++KRQTRFV
Sbjct: 290 DVQAVFDNIEDKYVSEQVTHNDGGPLVMNAFEMITLSQGLDLSALFDRQQEFVKRQTRFV 349

Query: 262 SRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVD 321
           SRKPA  I+++IE VAE MGL V ++N+K RLE  +  R++ FA VL+++EVAPSLFMVD
Sbjct: 350 SRKPAKTIVATIEVVAETMGLKVHSQNYKLRLEGVSSNRMSPFAVVLQVFEVAPSLFMVD 409

Query: 322 VRKAAGDTLEYHKFYKNFCGKLENIIWKPSE 353
           VRK AGDTLEYH+FYKN C K+E+IIW+P E
Sbjct: 410 VRKVAGDTLEYHRFYKNLCNKMESIIWRPIE 440

BLAST of Cucsat.G19232 vs. ExPASy Swiss-Prot
Match: Q9STV4 (CBL-interacting serine/threonine-protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CIPK8 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 2.3e-121
Identity = 208/331 (62.84%), Postives = 261/331 (78.85%), Query Frame = 0

Query: 24  KLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQKGVD 83
           +L E E+R+YFHQLID V +CHSKGVYHRDLKPENLLLD  GNLK+SDFGLSAL ++GV 
Sbjct: 103 RLSESEARKYFHQLIDGVDYCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSALPEQGVT 162

Query: 84  LLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKINA 143
           +L TTCGTPNYVAPEVL  +GY+GA ADIWSCGVILYVLMAGYLPF EMDLPTL  KI+ 
Sbjct: 163 ILKTTCGTPNYVAPEVLSHKGYNGAVADIWSCGVILYVLMAGYLPFDEMDLPTLYSKIDK 222

Query: 144 AEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLDDV 203
           AE+SCP +F+ GAKSLI +ILDPNP+ RI I EIRKD WF ++Y P +L + E VNLDDV
Sbjct: 223 AEFSCPSYFALGAKSLINRILDPNPETRITIAEIRKDEWFLKDYTPVQLIDYEHVNLDDV 282

Query: 204 RAVFDDIEDE-YVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFVS 263
            A FDD E++ Y  D T   D GPL +NAF++I LSQGLNL+ LFDR +D +K QTRF+S
Sbjct: 283 YAAFDDPEEQTYAQDGTR--DTGPLTLNAFDLIILSQGLNLATLFDRGKDSMKHQTRFIS 342

Query: 264 RKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVDV 323
            KPA+ ++SS+E V+++MG     RN+K R+E  +  + + F+ +LE+++VAPS+ MVD+
Sbjct: 343 HKPANVVLSSMEVVSQSMGFKTHIRNYKMRVEGLSANKTSHFSVILEVFKVAPSILMVDI 402

Query: 324 RKAAGDTLEYHKFYKNFCGKLENIIWKPSES 354
           + AAGD  EY KFYK FC KL++IIWKP ++
Sbjct: 403 QNAAGDAEEYLKFYKTFCSKLDDIIWKPPDA 431

BLAST of Cucsat.G19232 vs. ExPASy Swiss-Prot
Match: Q5JLD8 (CBL-interacting protein kinase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK8 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 6.4e-119
Identity = 209/325 (64.31%), Postives = 250/325 (76.92%), Query Frame = 0

Query: 24  KLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQKGVD 83
           +L E ++RRYF QLID V  CHSKGVYHRDLKPENLLLD  GNLK+SDFGLSA   +G  
Sbjct: 107 RLNEADARRYFQQLIDGVDFCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSAWPAQGGA 166

Query: 84  LLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKINA 143
           LL TTCGTPNYVAPEVL  +GYDGA AD WSCGVILYVL+AGYLPF E+DL TL  KI +
Sbjct: 167 LLRTTCGTPNYVAPEVLSHKGYDGALADTWSCGVILYVLLAGYLPFDEVDLTTLYGKIES 226

Query: 144 AEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLDDV 203
           AEYS P WF  GAKSLI +ILDPNP  RIRIEEIR D WF++NY P +  E EEVNLDDV
Sbjct: 227 AEYSFPAWFPNGAKSLIHRILDPNPDKRIRIEEIRNDEWFKKNYEPTREIESEEVNLDDV 286

Query: 204 RAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFVSR 263
            A FDD E++  AD T   + GPL +NAF++I LSQGLNL+ LFDRRQDY K Q RF+SR
Sbjct: 287 NAAFDDPEED--ADHTLDDEAGPLTLNAFDLIILSQGLNLAALFDRRQDYDKLQNRFLSR 346

Query: 264 KPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVDVR 323
           KPA  IMSS+E VA++MG     RN+K R+E     + +  A +LEI+EVAPS+FM++++
Sbjct: 347 KPAKVIMSSMEVVAQSMGYKTHIRNYKMRVEGLNANKTSHLAVMLEIFEVAPSIFMIELQ 406

Query: 324 KAAGDTLEYHKFYKNFCGKLENIIW 349
           +AAGDT +Y+KF  N+C KL++IIW
Sbjct: 407 RAAGDTSDYNKFINNYCSKLDDIIW 429

BLAST of Cucsat.G19232 vs. ExPASy Swiss-Prot
Match: Q84VQ3 (CBL-interacting serine/threonine-protein kinase 26 OS=Arabidopsis thaliana OX=3702 GN=CIPK26 PE=1 SV=2)

HSP 1 Score: 385.2 bits (988), Expect = 8.1e-106
Identity = 188/334 (56.29%), Postives = 240/334 (71.86%), Query Frame = 0

Query: 24  KLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ--KG 83
           +L E  +R+YF QLI+AV +CHS+GVYHRDLKPENLLLD  GNLKVSDFGLSALS+  +G
Sbjct: 107 RLKEENARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDAQGNLKVSDFGLSALSRQVRG 166

Query: 84  VDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNMKI 143
             LLHT CGTPNY APEVL  QGYDGA AD+WSCGVIL+VL+AGYLPF + +L TL  KI
Sbjct: 167 DGLLHTACGTPNYAAPEVLNDQGYDGATADLWSCGVILFVLLAGYLPFEDSNLMTLYKKI 226

Query: 144 NAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVNLD 203
            A EY CP W SPGAK+LI +ILDPNP  RI I E+  D WF++NY PA   E EE NLD
Sbjct: 227 IAGEYHCPPWLSPGAKNLIVRILDPNPMTRITIPEVLGDAWFKKNYKPAVFEEKEEANLD 286

Query: 204 DVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTRFV 263
           DV AVF D E+ +V   TEK ++ P  MNAFE+I++S+ L+L  LF+  + + KR+TRF 
Sbjct: 287 DVDAVFKDSEEHHV---TEKKEEQPTSMNAFELISMSRALDLGNLFEEEEGF-KRETRFA 346

Query: 264 SRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFMVD 323
           ++  A+ ++  IE  ++ +G ++Q +N+K RLE  T  R        EI++V+PSL M++
Sbjct: 347 AKGAANDLVQKIEEASKPLGFDIQKKNYKMRLENVTAGRKGNLRVATEIFQVSPSLHMIE 406

Query: 324 VRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVG 356
           VRK  GDTLE+HKFYK     L +++WK  ES G
Sbjct: 407 VRKTKGDTLEFHKFYKKLSTSLNDVVWKSGESSG 436

BLAST of Cucsat.G19232 vs. NCBI nr
Match: XP_011659826.1 (CBL-interacting serine/threonine-protein kinase 24 isoform X2 [Cucumis sativus] >KGN65636.2 hypothetical protein Csa_020014 [Cucumis sativus])

HSP 1 Score: 725 bits (1871), Expect = 6.89e-264
Identity = 356/356 (100.00%), Postives = 356/356 (100.00%), Query Frame = 0

Query: 1   MRCWLAGQRYILFLSLSQEVLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLL 60
           MRCWLAGQRYILFLSLSQEVLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLL
Sbjct: 1   MRCWLAGQRYILFLSLSQEVLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLL 60

Query: 61  LDDDGNLKVSDFGLSALSQKGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY 120
           LDDDGNLKVSDFGLSALSQKGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY
Sbjct: 61  LDDDGNLKVSDFGLSALSQKGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY 120

Query: 121 VLMAGYLPFSEMDLPTLNMKINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKD 180
           VLMAGYLPFSEMDLPTLNMKINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKD
Sbjct: 121 VLMAGYLPFSEMDLPTLNMKINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKD 180

Query: 181 PWFRRNYIPAKLGEDEEVNLDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQG 240
           PWFRRNYIPAKLGEDEEVNLDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQG
Sbjct: 181 PWFRRNYIPAKLGEDEEVNLDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQG 240

Query: 241 LNLSPLFDRRQDYIKRQTRFVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYR 300
           LNLSPLFDRRQDYIKRQTRFVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYR
Sbjct: 241 LNLSPLFDRRQDYIKRQTRFVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYR 300

Query: 301 VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN
Sbjct: 301 VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356

BLAST of Cucsat.G19232 vs. NCBI nr
Match: XP_011659824.1 (CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumis sativus] >XP_011659825.1 CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumis sativus])

HSP 1 Score: 686 bits (1771), Expect = 2.47e-247
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 101 VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 160

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM
Sbjct: 161 KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 220

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN
Sbjct: 221 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 280

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 281 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 340

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM
Sbjct: 341 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 400

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN
Sbjct: 401 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 437

BLAST of Cucsat.G19232 vs. NCBI nr
Match: XP_016899826.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 24 isoform X2 [Cucumis melo])

HSP 1 Score: 664 bits (1714), Expect = 6.35e-239
Identity = 323/337 (95.85%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 84  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 143

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GVDLLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE DLPTLNM
Sbjct: 144 QGVDLLHTTCGTPNYVAPEVLSGQGYDGAAADIWSCGVILYVLMAGYLPFSETDLPTLNM 203

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLI+KILDPNPK RIRIEEIRKDPWFRRNY+PAKLGEDEEVN
Sbjct: 204 KINAAEYSCPYWFSPGAKSLIEKILDPNPKTRIRIEEIRKDPWFRRNYVPAKLGEDEEVN 263

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQ+EK+DDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 264 LDDVRAVFDDIEDEYVADQSEKNDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 323

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSR+PA+FIMSSIEAVAE MGLNV TRNFKTRLERRTG+RVAQFAAVLEIYEVAPSLFM
Sbjct: 324 FVSRRPANFIMSSIEAVAETMGLNVHTRNFKTRLERRTGHRVAQFAAVLEIYEVAPSLFM 383

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 384 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 420

BLAST of Cucsat.G19232 vs. NCBI nr
Match: XP_008443989.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumis melo] >TYK06423.1 CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 664 bits (1714), Expect = 1.19e-238
Identity = 323/337 (95.85%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 101 VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 160

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GVDLLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE DLPTLNM
Sbjct: 161 QGVDLLHTTCGTPNYVAPEVLSGQGYDGAAADIWSCGVILYVLMAGYLPFSETDLPTLNM 220

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLI+KILDPNPK RIRIEEIRKDPWFRRNY+PAKLGEDEEVN
Sbjct: 221 KINAAEYSCPYWFSPGAKSLIEKILDPNPKTRIRIEEIRKDPWFRRNYVPAKLGEDEEVN 280

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQ+EK+DDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 281 LDDVRAVFDDIEDEYVADQSEKNDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 340

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSR+PA+FIMSSIEAVAE MGLNV TRNFKTRLERRTG+RVAQFAAVLEIYEVAPSLFM
Sbjct: 341 FVSRRPANFIMSSIEAVAETMGLNVHTRNFKTRLERRTGHRVAQFAAVLEIYEVAPSLFM 400

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 401 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 437

BLAST of Cucsat.G19232 vs. NCBI nr
Match: XP_038879116.1 (CBL-interacting protein kinase 24 isoform X4 [Benincasa hispida])

HSP 1 Score: 661 bits (1705), Expect = 1.33e-238
Identity = 330/356 (92.70%), Postives = 339/356 (95.22%), Query Frame = 0

Query: 1   MRCWLAGQRYILFLSLSQEVLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLL 60
           MRCWLAGQRYIL LSLS EVLQKKLPEIESRRYF QLIDAVAHCHSKGVYHRDLKPENLL
Sbjct: 1   MRCWLAGQRYILSLSLSLEVLQKKLPEIESRRYFQQLIDAVAHCHSKGVYHRDLKPENLL 60

Query: 61  LDDDGNLKVSDFGLSALSQKGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY 120
           LD DGNLKVSDFGLSALS++GV+LLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY
Sbjct: 61  LDADGNLKVSDFGLSALSRQGVELLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILY 120

Query: 121 VLMAGYLPFSEMDLPTLNMKINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKD 180
           VLMAGYLPFSE DLPTLNMKI+AAEYSCPYWFS GAKSLIQKILDPNPK RIRIEEIRKD
Sbjct: 121 VLMAGYLPFSETDLPTLNMKIHAAEYSCPYWFSSGAKSLIQKILDPNPKTRIRIEEIRKD 180

Query: 181 PWFRRNYIPAKLGEDEEVNLDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQG 240
           PWFRRNYIPAKLGEDEEVNLDDV+AVFDDIEDEYVADQ+EKSD GPLIMNAFEMITLSQG
Sbjct: 181 PWFRRNYIPAKLGEDEEVNLDDVQAVFDDIEDEYVADQSEKSDGGPLIMNAFEMITLSQG 240

Query: 241 LNLSPLFDRRQDYIKRQTRFVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYR 300
           LNLSPLFDR QDYIKRQTRFVS KPA+FIMS+IEAVAE MGLNV TRNFKTRLER TG+R
Sbjct: 241 LNLSPLFDRGQDYIKRQTRFVSCKPANFIMSTIEAVAETMGLNVHTRNFKTRLER-TGHR 300

Query: 301 VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFC KLENII KP ESV N
Sbjct: 301 VAQFAAVLEIYEVAPSLFMVDVRKAAGDTLEYHKFYKNFCAKLENIILKPLESVRN 355

BLAST of Cucsat.G19232 vs. ExPASy TrEMBL
Match: A0A1S4DV15 (CBL-interacting serine/threonine-protein kinase 24 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487442 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 3.08e-239
Identity = 323/337 (95.85%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 84  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 143

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GVDLLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE DLPTLNM
Sbjct: 144 QGVDLLHTTCGTPNYVAPEVLSGQGYDGAAADIWSCGVILYVLMAGYLPFSETDLPTLNM 203

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLI+KILDPNPK RIRIEEIRKDPWFRRNY+PAKLGEDEEVN
Sbjct: 204 KINAAEYSCPYWFSPGAKSLIEKILDPNPKTRIRIEEIRKDPWFRRNYVPAKLGEDEEVN 263

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQ+EK+DDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 264 LDDVRAVFDDIEDEYVADQSEKNDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 323

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSR+PA+FIMSSIEAVAE MGLNV TRNFKTRLERRTG+RVAQFAAVLEIYEVAPSLFM
Sbjct: 324 FVSRRPANFIMSSIEAVAETMGLNVHTRNFKTRLERRTGHRVAQFAAVLEIYEVAPSLFM 383

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 384 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 420

BLAST of Cucsat.G19232 vs. ExPASy TrEMBL
Match: A0A5D3C3C6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001260 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 5.76e-239
Identity = 323/337 (95.85%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 101 VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 160

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GVDLLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE DLPTLNM
Sbjct: 161 QGVDLLHTTCGTPNYVAPEVLSGQGYDGAAADIWSCGVILYVLMAGYLPFSETDLPTLNM 220

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLI+KILDPNPK RIRIEEIRKDPWFRRNY+PAKLGEDEEVN
Sbjct: 221 KINAAEYSCPYWFSPGAKSLIEKILDPNPKTRIRIEEIRKDPWFRRNYVPAKLGEDEEVN 280

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQ+EK+DDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 281 LDDVRAVFDDIEDEYVADQSEKNDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 340

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSR+PA+FIMSSIEAVAE MGLNV TRNFKTRLERRTG+RVAQFAAVLEIYEVAPSLFM
Sbjct: 341 FVSRRPANFIMSSIEAVAETMGLNVHTRNFKTRLERRTGHRVAQFAAVLEIYEVAPSLFM 400

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 401 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 437

BLAST of Cucsat.G19232 vs. ExPASy TrEMBL
Match: A0A1S3BA51 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103487442 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 5.76e-239
Identity = 323/337 (95.85%), Postives = 331/337 (98.22%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ
Sbjct: 101 VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 160

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GVDLLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE DLPTLNM
Sbjct: 161 QGVDLLHTTCGTPNYVAPEVLSGQGYDGAAADIWSCGVILYVLMAGYLPFSETDLPTLNM 220

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPGAKSLI+KILDPNPK RIRIEEIRKDPWFRRNY+PAKLGEDEEVN
Sbjct: 221 KINAAEYSCPYWFSPGAKSLIEKILDPNPKTRIRIEEIRKDPWFRRNYVPAKLGEDEEVN 280

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFDDIEDEYVADQ+EK+DDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 281 LDDVRAVFDDIEDEYVADQSEKNDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 340

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSR+PA+FIMSSIEAVAE MGLNV TRNFKTRLERRTG+RVAQFAAVLEIYEVAPSLFM
Sbjct: 341 FVSRRPANFIMSSIEAVAETMGLNVHTRNFKTRLERRTGHRVAQFAAVLEIYEVAPSLFM 400

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 401 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 437

BLAST of Cucsat.G19232 vs. ExPASy TrEMBL
Match: A0A6J1JHP0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111485187 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 6.16e-227
Identity = 309/337 (91.69%), Postives = 321/337 (95.25%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VL KK+PE ESRRYF QLIDAVAHCHSKGVYHRDLKPENLLLD DGNLKVSDFGLSA+SQ
Sbjct: 101 VLHKKIPEFESRRYFQQLIDAVAHCHSKGVYHRDLKPENLLLDADGNLKVSDFGLSAVSQ 160

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GV+LLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE  LPTLNM
Sbjct: 161 QGVELLHTTCGTPNYVAPEVLRGQGYDGAAADIWSCGVILYVLMAGYLPFSESGLPTLNM 220

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPG KSLIQKILDPNPK RIRIEEIRKDPWFRRNY+PAKLG+ EEVN
Sbjct: 221 KINAAEYSCPYWFSPGVKSLIQKILDPNPKTRIRIEEIRKDPWFRRNYMPAKLGKVEEVN 280

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFD IE+EYV DQ+EKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 281 LDDVRAVFDGIENEYVVDQSEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 340

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSRKPA+FIMS+IE VAEAMGLNV TRNFKTRLERRTG+R +QFAAVLEIYEVAPSLFM
Sbjct: 341 FVSRKPANFIMSTIEVVAEAMGLNVHTRNFKTRLERRTGHRASQFAAVLEIYEVAPSLFM 400

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 401 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 437

BLAST of Cucsat.G19232 vs. ExPASy TrEMBL
Match: A0A6J1JJN3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111485187 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 1.10e-226
Identity = 309/337 (91.69%), Postives = 321/337 (95.25%), Query Frame = 0

Query: 20  VLQKKLPEIESRRYFHQLIDAVAHCHSKGVYHRDLKPENLLLDDDGNLKVSDFGLSALSQ 79
           VL KK+PE ESRRYF QLIDAVAHCHSKGVYHRDLKPENLLLD DGNLKVSDFGLSA+SQ
Sbjct: 117 VLHKKIPEFESRRYFQQLIDAVAHCHSKGVYHRDLKPENLLLDADGNLKVSDFGLSAVSQ 176

Query: 80  KGVDLLHTTCGTPNYVAPEVLGGQGYDGAAADIWSCGVILYVLMAGYLPFSEMDLPTLNM 139
           +GV+LLHTTCGTPNYVAPEVL GQGYDGAAADIWSCGVILYVLMAGYLPFSE  LPTLNM
Sbjct: 177 QGVELLHTTCGTPNYVAPEVLRGQGYDGAAADIWSCGVILYVLMAGYLPFSESGLPTLNM 236

Query: 140 KINAAEYSCPYWFSPGAKSLIQKILDPNPKARIRIEEIRKDPWFRRNYIPAKLGEDEEVN 199
           KINAAEYSCPYWFSPG KSLIQKILDPNPK RIRIEEIRKDPWFRRNY+PAKLG+ EEVN
Sbjct: 237 KINAAEYSCPYWFSPGVKSLIQKILDPNPKTRIRIEEIRKDPWFRRNYMPAKLGKVEEVN 296

Query: 200 LDDVRAVFDDIEDEYVADQTEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 259
           LDDVRAVFD IE+EYV DQ+EKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR
Sbjct: 297 LDDVRAVFDGIENEYVVDQSEKSDDGPLIMNAFEMITLSQGLNLSPLFDRRQDYIKRQTR 356

Query: 260 FVSRKPASFIMSSIEAVAEAMGLNVQTRNFKTRLERRTGYRVAQFAAVLEIYEVAPSLFM 319
           FVSRKPA+FIMS+IE VAEAMGLNV TRNFKTRLERRTG+R +QFAAVLEIYEVAPSLFM
Sbjct: 357 FVSRKPANFIMSTIEVVAEAMGLNVHTRNFKTRLERRTGHRASQFAAVLEIYEVAPSLFM 416

Query: 320 VDVRKAAGDTLEYHKFYKNFCGKLENIIWKPSESVGN 356
           VDVRKAAGDTLEYHKFYKNFC KLENIIWKPSESVGN
Sbjct: 417 VDVRKAAGDTLEYHKFYKNFCAKLENIIWKPSESVGN 453

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LDI32.2e-14373.11CBL-interacting serine/threonine-protein kinase 24 OS=Arabidopsis thaliana OX=37... [more]
Q69Q471.2e-14171.60CBL-interacting protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9STV42.3e-12162.84CBL-interacting serine/threonine-protein kinase 8 OS=Arabidopsis thaliana OX=370... [more]
Q5JLD86.4e-11964.31CBL-interacting protein kinase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q84VQ38.1e-10656.29CBL-interacting serine/threonine-protein kinase 26 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
XP_011659826.16.89e-264100.00CBL-interacting serine/threonine-protein kinase 24 isoform X2 [Cucumis sativus] ... [more]
XP_011659824.12.47e-247100.00CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumis sativus] ... [more]
XP_016899826.16.35e-23995.85PREDICTED: CBL-interacting serine/threonine-protein kinase 24 isoform X2 [Cucumi... [more]
XP_008443989.11.19e-23895.85PREDICTED: CBL-interacting serine/threonine-protein kinase 24 isoform X1 [Cucumi... [more]
XP_038879116.11.33e-23892.70CBL-interacting protein kinase 24 isoform X4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A1S4DV153.08e-23995.85CBL-interacting serine/threonine-protein kinase 24 isoform X2 OS=Cucumis melo OX... [more]
A0A5D3C3C65.76e-23995.85Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BA515.76e-23995.85Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1JHP06.16e-22791.69Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1JJN31.10e-22691.69Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 101..227
e-value: 2.5E-49
score: 168.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 1..69
e-value: 2.6E-10
score: 42.0
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 1..230
NoneNo IPR availablePANTHERPTHR43895:SF114NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 1..230
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 108..218
e-value: 7.09495E-48
score: 151.956
IPR004041NAF domainPFAMPF03822NAFcoord: 104..163
e-value: 4.7E-20
score: 71.5
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 102..126
score: 12.128263
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 2..69

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G19232.T3Cucsat.G19232.T3mRNA
Cucsat.G19232.T2Cucsat.G19232.T2mRNA
Cucsat.G19232.T1Cucsat.G19232.T1mRNA
Cucsat.G19232.T4Cucsat.G19232.T4mRNA
Cucsat.G19232.T6Cucsat.G19232.T6mRNA
Cucsat.G19232.T8Cucsat.G19232.T8mRNA
Cucsat.G19232.T5Cucsat.G19232.T5mRNA
Cucsat.G19232.T13Cucsat.G19232.T13mRNA
Cucsat.G19232.T10Cucsat.G19232.T10mRNA
Cucsat.G19232.T12Cucsat.G19232.T12mRNA
Cucsat.G19232.T17Cucsat.G19232.T17mRNA
Cucsat.G19232.T16Cucsat.G19232.T16mRNA
Cucsat.G19232.T14Cucsat.G19232.T14mRNA
Cucsat.G19232.T9Cucsat.G19232.T9mRNA
Cucsat.G19232.T7Cucsat.G19232.T7mRNA
Cucsat.G19232.T11Cucsat.G19232.T11mRNA
Cucsat.G19232.T15Cucsat.G19232.T15mRNA
Cucsat.G19232.T18Cucsat.G19232.T18mRNA
Cucsat.G19232.T20Cucsat.G19232.T20mRNA
Cucsat.G19232.T19Cucsat.G19232.T19mRNA
Cucsat.G19232.T21Cucsat.G19232.T21mRNA
Cucsat.G19232.T22Cucsat.G19232.T22mRNA
Cucsat.G19232.T23Cucsat.G19232.T23mRNA
Cucsat.G19232.T24Cucsat.G19232.T24mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009651 response to salt stress
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity