Cucsat.G1918 (gene) Cucumber (B10) v3

Overview
NameCucsat.G1918
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionAspartyl protease family protein 1-like
Locationctg1002: 4221105 .. 4224111 (-)
RNA-Seq ExpressionCucsat.G1918
SyntenyCucsat.G1918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTCACTTTCCCGGGTTTCTCACCGGAACTTTTTGTATTACTCTATTCGCCGTCCATGGCGTCCACCTTCAGTTCCGGCGCTCAAATGTTACTGGTTCTTTCTGTTTTTATTCTCGCCGGAAGCCTAAGAAGCGGCGACGCGGCTTCGTTTAAGTTCGACATCCACCATCGATTTTCGGACTCAATAAAAGGGATTTTCCACTCTGAAGGTTTACCGGAGAAACATACCCCTGGATATTATGCTACTATGGTTCACCGTGACCGGTTAGTTCGTGGTCGGCGATTGGCAGCTAGTGATGTTGATACGCAGCTGACGTTCGCTTATGGCAACGATACCGCCTTCATCCCTGACTTGGGATTGTAATGCTTCTTATTCTCTCTCTCTCTCTCTCTCCATTTTCTTTAATTTCTGTTTGTTTTGATTTATTTTCTAATCTTTGTCGATTATCTTTGATTAAATTGTTCTATCTTGTTTCCTTCCATTTGGGGGTTTTCAGTTTATACTATGCTAATGTTTCGGTCGGAACGCCGTCGTTAGATTTCCTTGTGGCGTTGGACACTGGAAGCGATTTGTTCTGGTTACCGTGTGAATGTTCTAGTTGTTTTACTTATTTGAACACATCAAACGGAGGAAAGGTATTTAAACCGACTTTGCAAATTTTGAAGTTGATGTTTTCTCTGATGAAATCAATTGGTTGACTCTTGTTTTGTTTATCTTTCTACAGTTTATGTTGAATCATTATAGTCCAAATGATTCAACAACGAGCTCAACTGTTCCCTGCACTAGCTCTTTGTGCAACCGATGTACTTCAAACCAAAATGTTTGTCCTTATGAAATGAGATACCTGTCTGCTAATACCTCCTCGATTGGCTACTTGGTTGAGGACGTATTGCACTTGGCCACTGATGATTCACTACTCAAACCTGTTGAGGCTAAGATTACTTTTGGGTGAGTTAATTATGTTTCATCTTTCTGTCTGTATCTTTTTCCTCTAGATTATATCTTGTGCTGAATTTTGTTCTGTTTTTAATAGATTGTTTCTTTTCTTCTAACATTGCTTTTTTTCTTTGCTTAGGAATCAATAATAGTTTATGGTAGAGTAGGGATATAATTATTACACTAACCATCGGCTTAAGTTTTTATTCTCGTCAATTTAAAAACATGGCCTAGAGCAAAGAGGTCTTATATTTGAACTCTTGTAATGACATTGTCAATTAAGATAGCATGAATCAATAAGCAATTATATTCGATTTTGTAACTTAACTTGGTCTAGAGAAAAGAGATCCTTTACTTTTTAAGGACACAGTTGTTCAACCTATAGACAATGTTTAATTAAAGATGTAGCAAAGAGGTTATGTATTTCGAACTTCTTCCAATTAAGGTTGGATCATGGATGGAATAATAAAATATTTTTGACTTTGTAACTTAATCATTGTTGCAAGAGAAGGGTGTTTAATGATCTTATAGAAAGCATCTGCAGTTTGTTGTGTTGTTGCCCCTAAATGTGATTCTTCATGGCAGGTGCGGTACGGTCCAGACTGGTATATTTGCAACAACTGCAGCTCCCAATGGTCTTATTGGGCTTGGCATGGAAAAGATATCAGTTCCAAGCTTCTTAGCAGACCAAGGACTTACCTCAAATTCATTCTCCATGTGTTTCGGAGCTGATGGTTATGGGAGAATCGATTTTGGGGACACAGGCCCAGCAGACCAAAAACAAACCCCCTTCAATACCATGCTCGAGTAGTAAGAACTCTTCACTTCTTAAACCATGCATATTCAAATTGTGCAAAAAGTACAATTTCTTACACCTTCATTTCTCTTGGAGAAATTGGTTTTTCTAATTGGAATGTCACGTTCATTTTGGCAGTCAATCCTACAATGTCACCTTCAATGTGATAAACGTGGGAGGAGAACCCAACGATGTTCCATTTACTGCAATTTTTGACTCTGGAACCTCGTTCACGTACCTAACCGAACCAGCTTACTCAACTATTACTAAGCAAGTAAGTGATCTGCAGTTTCAACCATTGAAAAATGTCATATGGTTTCATGCGATCTCTGATATTTACATCGAATTATTGTGCAGATGGACGCAGGGATGAAGTTAAAGCGCTATTCATTATTTGGTCCTAATTTCCCATTTGAATACTGCTACGAAATTCCGTAAGTAGCTGATGGTTTATTATATATAACGAAGCCAGCTATTCTCTTTCAATACACATTCCTGAATTCTGATTCATACCCTTTTCATATTCAGTCCAGGTGCAAAAGAATTTCAATATCTGACGCTGAATTTTACAATGAAGGGTGGAGATGAATTTACCCCTACAGATATTTTTGTTTTTCTTCCTGTTGATGTGAGTACTATGAACATTATATTTGAAGTAACTTTCAAATTTTAGACAAGAGAGAGCTTTGGCTTTTATGGCTTATTATCTCACTCCTACATGCTATCTTTGGAGCTTTTTGTTGATCTGGGTTTGCTGTTTTTTGGTCTTCAGGAAACTACCCATGTTGCTTGTCTAGCCATTGCCAAAAGCACCGATATTGATCTGATTGGACGTGAGTCCTCCCTTCATGAATTACAAATTTGATCCCTATAATTTTTTTTTTAAAAAAATAGTGATTTTTAATCAAACTATATGAACTAAAATCCAACCTTCGAAATCGAGATTGAGTTCGTGCTTATGATCTAATGGTCTTTCCTTCACGTCTTCTTGCAGAAAACTTCATGACTGGTTATCGTATTACCTTCAATCGTGATCAGATGGTGTTGGGATGGAGTTCTTCAGACTGTGAGTATTATTTCACCTGAAAACTCTTCTTTCTTCTACTAGTCCTTTTTTTTCAATGTCAATGAAAATTGTTTTCTTAACTCCAGGTTACGACAATGGCGTCGGCACTCCCTCCGGCGATACCCCTCCGGCTGATTCTCCCTCCGACTCCCCTCCGACTGATTCCCCACCATCCGACTCCCCTCCGACTGATTCCCCACCGTCCGA

Coding sequence (CDS)

CTCACTTTCCCGGGTTTCTCACCGGAACTTTTTGTATTACTCTATTCGCCGTCCATGGCGTCCACCTTCAGTTCCGGCGCTCAAATGTTACTGGTTCTTTCTGTTTTTATTCTCGCCGGAAGCCTAAGAAGCGGCGACGCGGCTTCGTTTAAGTTCGACATCCACCATCGATTTTCGGACTCAATAAAAGGGATTTTCCACTCTGAAGGTTTACCGGAGAAACATACCCCTGGATATTATGCTACTATGGTTCACCGTGACCGGTTAGTTCGTGGTCGGCGATTGGCAGCTAGTGATGTTGATACGCAGCTGACGTTCGCTTATGGCAACGATACCGCCTTCATCCCTGACTTGGGATTTTTATACTATGCTAATGTTTCGGTCGGAACGCCGTCGTTAGATTTCCTTGTGGCGTTGGACACTGGAAGCGATTTGTTCTGGTTACCGTGTGAATGTTCTAGTTGTTTTACTTATTTGAACACATCAAACGGAGGAAAGTTTATGTTGAATCATTATAGTCCAAATGATTCAACAACGAGCTCAACTGTTCCCTGCACTAGCTCTTTGTGCAACCGATGTACTTCAAACCAAAATGTTTGTCCTTATGAAATGAGATACCTGTCTGCTAATACCTCCTCGATTGGCTACTTGGTTGAGGACGTATTGCACTTGGCCACTGATGATTCACTACTCAAACCTGTTGAGGCTAAGATTACTTTTGGGTGCGGTACGGTCCAGACTGGTATATTTGCAACAACTGCAGCTCCCAATGGTCTTATTGGGCTTGGCATGGAAAAGATATCAGTTCCAAGCTTCTTAGCAGACCAAGGACTTACCTCAAATTCATTCTCCATGTGTTTCGGAGCTGATGGTTATGGGAGAATCGATTTTGGGGACACAGGCCCAGCAGACCAAAAACAAACCCCCTTCAATACCATGCTCGAGTATCAATCCTACAATGTCACCTTCAATGTGATAAACGTGGGAGGAGAACCCAACGATGTTCCATTTACTGCAATTTTTGACTCTGGAACCTCGTTCACGTACCTAACCGAACCAGCTTACTCAACTATTACTAAGCAAATGGACGCAGGGATGAAGTTAAAGCGCTATTCATTATTTGGTCCTAATTTCCCATTTGAATACTGCTACGAAATTCCTCCAGGTGCAAAAGAATTTCAATATCTGACGCTGAATTTTACAATGAAGGGTGGAGATGAATTTACCCCTACAGATATTTTTGTTTTTCTTCCTGTTGATGAAACTACCCATGTTGCTTGTCTAGCCATTGCCAAAAGCACCGATATTGATCTGATTGGACAAAACTTCATGACTGGTTATCGTATTACCTTCAATCGTGATCAGATGGTGTTGGGATGGAGTTCTTCAGACTGTTACGACAATGGCGTCGGCACTCCCTCCGGCGATACCCCTCCGGCTGATTCTCCCTCCGACTCCCCTCCGACTGATTCCCCACCATCCGACTCCCCTCCGACTGATTCCCCACCGTCC

Protein sequence

LTFPGFSPELFVLLYSPSMASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPGYYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNGLIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQSYNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSDSPPTDSPPS
Homology
BLAST of Cucsat.G1918 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 8.2e-128
Identity = 245/488 (50.20%), Postives = 318/488 (65.16%), Query Frame = 0

Query: 25  SGAQMLLVLSVFILAGSL---RSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPGYYA 84
           S  ++L +  + +LA S    R      F F+ HHRFSD + G+   +GLP + +  YY 
Sbjct: 5   SSCRILFLGLLILLASSWVLDRCEGFGEFGFEFHHRFSDQVVGVLPGDGLPNRDSSKYYR 64

Query: 85  TMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDT 144
            M HRDRL+RGRRLA  D  + +TF+ GN+T  +  LGFL+YANV+VGTPS  F+VALDT
Sbjct: 65  VMAHRDRLIRGRRLANED-QSLVTFSDGNETVRVDALGFLHYANVTVGTPSDWFMVALDT 124

Query: 145 GSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTSNQN 204
           GSDLFWLPC+C++C   L    G    LN YSPN S+TS+ VPC S+LC   +RC S ++
Sbjct: 125 GSDLFWLPCDCTNCVRELKAPGGSSLDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPES 184

Query: 205 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 264
            CPY++RYLS  TSS G LVEDVLHL ++D   K + A++TFGCG VQTG+F   AAPNG
Sbjct: 185 DCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKSSKAIPARVTFGCGQVQTGVFHDGAAPNG 244

Query: 265 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 324
           L GLG+E ISVPS LA +G+ +NSFSMCFG DG GRI FGD G  DQ++TP N    + +
Sbjct: 245 LFGLGLEDISVPSVLAKEGIAANSFSMCFGNDGAGRISFGDKGSVDQRETPLNIRQPHPT 304

Query: 325 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 384
           YN+T   I+VGG   D+ F A+FDSGTSFTYLT+ AY+ I++  ++    KRY       
Sbjct: 305 YNITVTKISVGGNTGDLEFDAVFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSEL 364

Query: 385 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 444
           PFEYCY + P    FQY  +N TMKGG  +      V +P+ + T V CLAI K  DI +
Sbjct: 365 PFEYCYALSPNKDSFQYPAVNLTMKGGSSYPVYHPLVVIPMKD-TDVYCLAIMKIEDISI 424

Query: 445 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDS--PPTDS--PPS 503
           IGQNFMTGYR+ F+R++++LGW  SDCY    G  S  T P++  S S  PP  S  P +
Sbjct: 425 IGQNFMTGYRVVFDREKLILGWKESDCY---TGETSARTLPSNRSSSSARPPASSFDPEA 484

BLAST of Cucsat.G1918 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 9.8e-65
Identity = 179/477 (37.53%), Postives = 248/477 (51.99%), Query Frame = 0

Query: 47  AASFKFDIHHRFSD----SIKGIFHSEGLPEKHTPGYYATMVHRDRLVRGRRLAASDVDT 106
           A+ F   + HRFSD    SIK    S+ LP K +  YY  +   D   R +R+       
Sbjct: 22  ASLFSSRLIHRFSDEGRASIKTPSSSDSLPNKQSLEYYRLLAESD--FRRQRMNLGAKVQ 81

Query: 107 QLTFAYGNDT-AFIPDLGFLYYANVSVGTPSLDFLVALDTGSDLFWLPCECSSCFTYLNT 166
            L  + G+ T +   D G+L+Y  + +GTPS+ FLVALDTGS+L W+PC C  C    +T
Sbjct: 82  SLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTGSNLLWIPCNCVQCAPLTST 141

Query: 167 --SNGGKFMLNHYSPNDSTTSSTVPCTSSLCNR---CTSNQNVCPYEMRYLSANTSSIGY 226
             S+     LN Y+P+ S+TS    C+  LC+    C S +  CPY + YLS NTSS G 
Sbjct: 142 YYSSLATKDLNEYNPSSSSTSKVFLCSHKLCDSASDCESPKEQCPYTVNYLSGNTSSSGL 201

Query: 227 LVEDVLHLA--TDDSLL---KPVEAKITFGCGTVQTGIFATTAAPNGLIGLGMEKISVPS 286
           LVED+LHL   T++ L+     V+A++  GCG  Q+G +    AP+GL+GLG  +ISVPS
Sbjct: 202 LVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQSGDYLDGVAPDGLMGLGPAEISVPS 261

Query: 287 FLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTM--LEYQSYNVTFNVINVG 346
           FL+  GL  NSFS+CF  +  GRI FGD GP+ Q+ TPF  +   +Y  Y V      +G
Sbjct: 262 FLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQSTPFLQLDNNKYSGYIVGVEACCIG 321

Query: 347 GE-PNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFEYCYEIPP 406
                   FT   DSG SFTYL E  Y  +  ++D  +     +  G +  +EYCYE   
Sbjct: 322 NSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEIDRHINATSKNFEGVS--WEYCYE--- 381

Query: 407 GAKEFQYLTLNFTMKGGDEFT-PTDIFVFLPVDETTHVACLAIAKS--TDIDLIGQNFMT 466
            + E +   +       + F     +FVF          CL I+ S    I  IGQN+M 
Sbjct: 382 SSAEPKVPAIKLKFSHNNTFVIHKPLFVFQQSQGLVQF-CLPISPSGQEGIGSIGQNYMR 441

Query: 467 GYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSDSPPTDSP 503
           GYR+ F+R+ M LGWS S C ++ +  P   +P + S  +  PTD   S      SP
Sbjct: 442 GYRMVFDRENMKLGWSPSKCQEDKIEPPQA-SPGSTSSPNPLPTDEQQSRGGHAVSP 489

BLAST of Cucsat.G1918 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.8e-24
Identity = 113/468 (24.15%), Postives = 195/468 (41.67%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           M +T        +V  VF+L   + SG   +F F++ H+F+   K +             
Sbjct: 1   MVTTMDPSRISRIVAVVFVLVIQVVSG---NFVFNVTHKFAGKEKQL------------- 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
             + +   D     R LA  D+        G D+    D   LY+  + +G+P  ++ V 
Sbjct: 61  --SELKSHDSFRHARMLANIDL------PLGGDSR--ADSIGLYFTKIKLGSPPKEYYVQ 120

Query: 139 LDTGSDLFWLPC-ECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQ 198
           +DTGSD+ W+ C  C  C   + T  G    L+ Y    S+TS  V C    C+    ++
Sbjct: 121 VDTGSDILWVNCAPCPKC--PVKTDLG--IPLSLYDSKTSSTSKNVGCEDDFCSFIMQSE 180

Query: 199 NV-----CPYEMRYLSANTSSIGYLVEDV-LHLATDDSLLKPVEAKITFGCGTVQTG-IF 258
                  C Y + Y   +TS   ++ +++ L   T +    P+  ++ FGCG  Q+G + 
Sbjct: 181 TCGAKKPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLG 240

Query: 259 ATTAAPNGLIGLGMEKISVPSFLADQGLTSNSFSMCF-GADGYGRIDFGDTGPADQKQTP 318
            T +A +G++G G    S+ S LA  G T   FS C    +G G    G+      K TP
Sbjct: 241 QTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFAVGEVESPVVKTTP 300

Query: 319 FNTMLEYQSYNVTFNVINVGGEPNDVPFT---------AIFDSGTSFTYLTEPAYSTITK 378
              +     YNV    ++V G+P D+P +          I DSGT+  YL +  Y+++ +
Sbjct: 301 I--VPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIE 360

Query: 379 QMDAGMKLKRYSLFGPNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVD 438
           ++ A  ++K + +      F +        K F  + L+F         P D    L  D
Sbjct: 361 KITAKQQVKLHMVQETFACFSFTSNTD---KAFPVVNLHFEDSLKLSVYPHDYLFSLRED 420

Query: 439 ETT---HVACLAIAKSTDIDLIGQNFMTGYRITFNRDQMVLGWSSSDC 466
                     +      D+ L+G   ++   + ++ +  V+GW+  +C
Sbjct: 421 MYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADHNC 433

BLAST of Cucsat.G1918 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.9e-23
Identity = 112/443 (25.28%), Postives = 189/443 (42.66%), Query Frame = 0

Query: 47  AASFKFDIHHRFSDSIKGIFHSEGLPEKHTPGYYATMVHRDRLVRGRRLAASDVDTQLTF 106
           +A+F F   H+F+   K + H +                 D     R LA+ D+      
Sbjct: 22  SANFVFKAQHKFAGKKKNLEHFKS---------------HDTRRHSRMLASIDL------ 81

Query: 107 AYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSDLFWLPCE-CSSCFTYLNTSNGG 166
             G D+  +  +G LY+  + +G+P  ++ V +DTGSD+ W+ C+ C  C T  N +   
Sbjct: 82  PLGGDSR-VDSVG-LYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNLN--- 141

Query: 167 KFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQNV-----CPYEMRYLSANTSSIGYLVED 226
            F L+ +  N S+TS  V C    C+  + + +      C Y + Y   +TS  G  + D
Sbjct: 142 -FRLSLFDMNASSTSKKVGCDDDFCSFISQSDSCQPALGCSYHIVYADESTSD-GKFIRD 201

Query: 227 VLHL--ATDDSLLKPVEAKITFGCGTVQTGIFAT-TAAPNGLIGLGMEKISVPSFLADQG 286
           +L L   T D    P+  ++ FGCG+ Q+G      +A +G++G G    SV S LA  G
Sbjct: 202 MLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATG 261

Query: 287 LTSNSFSMCF-GADGYGRIDFGDTGPADQKQTPFNTMLEYQSYNVTFNVINVGGEPNDVP 346
                FS C     G G    G       K TP   +     YNV    ++V G   D+P
Sbjct: 262 DAKRVFSHCLDNVKGGGIFAVGVVDSPKVKTTPM--VPNQMHYNVMLMGMDVDGTSLDLP 321

Query: 347 FT------AIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFEYCYEI---- 406
            +       I DSGT+  Y  +  Y ++ + + A   +K + +      F +   +    
Sbjct: 322 RSIVRNGGTIVDSGTTLAYFPKVLYDSLIETILARQPVKLHIVEETFQCFSFSTNVDEAF 381

Query: 407 PPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTD----IDLIGQN 466
           PP + EF+  ++  T+   D       ++F   +E       A   +TD    + L+G  
Sbjct: 382 PPVSFEFED-SVKLTVYPHD-------YLFTLEEELYCFGWQAGGLTTDERSEVILLGDL 426

BLAST of Cucsat.G1918 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 5.5e-23
Identity = 95/366 (25.96%), Postives = 159/366 (43.44%), Query Frame = 0

Query: 122 YYANVSVGTPSLDFLVALDTGSDLFWLPCE-CSSCFTYLNTSNGGKFMLNHYSPNDSTTS 181
           Y  N+S+GTP+  F   +DTGSDL W  C+ C+ CF               ++P  S++ 
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPI---------FNPQGSSSF 154

Query: 182 STVPCTSSLCNRCTS---NQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAK 241
           ST+PC+S LC   +S   + N C Y   Y    + + G +  + L   T  S+  P    
Sbjct: 155 STLPCSSQLCQALSSPTCSNNFCQYTYGY-GDGSETQGSMGTETL---TFGSVSIP---N 214

Query: 242 ITFGCGTVQTGIFATTAAPNGLIGLGMEKISVPSFLADQGLTSNSFSMC-FGADGYGRID 301
           ITFGCG    G      A  GL+G+G   +S+PS L    +T  S+ M   G+     + 
Sbjct: 215 ITFGCGENNQGFGQGNGA--GLVGMGRGPLSLPSQL---DVTKFSYCMTPIGSSTPSNLL 274

Query: 302 FGDTGPADQKQTPFNTMLEYQS----YNVTFNVINVGG------------EPNDVPFTAI 361
            G    +    +P  T+++       Y +T N ++VG               N+     I
Sbjct: 275 LGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGII 334

Query: 362 FDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFEYCYEIPPGAKEFQYLTLNF 421
            DSGT+ TY    AY ++ ++  + + L   +  G +  F+ C++ P      Q  T   
Sbjct: 335 IDSGTTLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSNLQIPTFVM 394

Query: 422 TMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTD-IDLIGQNFMTGYRITFNRDQMVLG 466
              GGD   P++ +   P   +  + CLA+  S+  + + G        + ++    V+ 
Sbjct: 395 HFDGGDLELPSENYFISP---SNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVS 434

BLAST of Cucsat.G1918 vs. NCBI nr
Match: XP_031742572.1 (aspartyl protease family protein 1 [Cucumis sativus] >KGN48951.1 hypothetical protein Csa_003569 [Cucumis sativus])

HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 485/486 (99.79%), Postives = 485/486 (99.79%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN
Sbjct: 121 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
           VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF
Sbjct: 301 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL
Sbjct: 361 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSDSPP 498
           IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPS SPP
Sbjct: 421 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSVSPP 480

Query: 499 TDSPPS 504
           TDSPPS
Sbjct: 481 TDSPPS 486

BLAST of Cucsat.G1918 vs. NCBI nr
Match: KAA0025642.1 (aspartyl protease family protein 1-like [Cucumis melo var. makuwa] >TYK12515.1 aspartyl protease family protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 833 bits (2153), Expect = 5.26e-301
Identity = 418/483 (86.54%), Postives = 447/483 (92.55%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLL+LSVFILAGSLRSGDAASFKF+IHHRFSDS+K + HSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLLLSVFILAGSLRSGDAASFKFNIHHRFSDSVKEVLHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAA++ DTQLTFAYGNDT FI +LGFLYYANVSVGTPS+DFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAATNDDTQLTFAYGNDTVFIAELGFLYYANVSVGTPSVDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSC TYLNTSNGGKFMLNHYSPNDSTTSS VPCTSSLC +CTSN+N
Sbjct: 121 LDTGSDLFWLPCECSSCATYLNTSNGGKFMLNHYSPNDSTTSSIVPCTSSLCGQCTSNKN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
           +CPYE+ Y+SANTSSIGYLVEDVLHLA DD+LLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 ICPYEINYVSANTSSIGYLVEDVLHLAADDALLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTS+SFSMCFG DGYGRIDFGDTGPA QKQTPFNTML +QS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSDSFSMCFGLDGYGRIDFGDTGPAGQKQTPFNTML-HQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFN INVGG+ N+VPFTAIFDSGTSFTYLTEP YSTI++QMDAGMKLKR+S FGP+F
Sbjct: 301 YNVTFNEINVGGKTNNVPFTAIFDSGTSFTYLTEPTYSTISEQMDAGMKLKRFS-FGPSF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PF+YCYEI P AKE +   LNFTM+GGDEF P DIFV +PVDET   ACLA+AKSTDIDL
Sbjct: 361 PFQYCYEISPSAKEIRRPQLNFTMEGGDEFVPIDIFVVIPVDETKSAACLALAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP-SDSPPTDSPPSDSP 498
           IGQNFMTGYRI FNR +MVLGWS SDCYDNG  TPS D+PPADSP SDSPPTDSPPSDSP
Sbjct: 421 IGQNFMTGYRIIFNRGEMVLGWSPSDCYDNGDSTPS-DSPPADSPPSDSPPTDSPPSDSP 480

Query: 499 PTD 500
           PTD
Sbjct: 481 PTD 480

BLAST of Cucsat.G1918 vs. NCBI nr
Match: XP_016899154.1 (PREDICTED: aspartyl protease family protein 1-like [Cucumis melo])

HSP 1 Score: 832 bits (2150), Expect = 7.72e-301
Identity = 418/483 (86.54%), Postives = 446/483 (92.34%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLL+LSVFILAGSLRSGDAASFKF+IHHRFSDS+K + HSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLLLSVFILAGSLRSGDAASFKFNIHHRFSDSVKEVLHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAA++ DTQLTFAYGNDT FI +LGFLYYANVSVGTPS+DFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAATNDDTQLTFAYGNDTVFIAELGFLYYANVSVGTPSVDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSC TYLNTSNGGKFMLNHYSPNDSTTSS VPCTSSLC +CTSN+N
Sbjct: 121 LDTGSDLFWLPCECSSCATYLNTSNGGKFMLNHYSPNDSTTSSIVPCTSSLCGQCTSNKN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
            CPYE+ Y+SANTSSIGYLVEDVLHLA DD+LLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 TCPYEINYVSANTSSIGYLVEDVLHLAADDALLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTS+SFSMCFG DGYGRIDFGDTGPA QKQTPFNTML +QS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSDSFSMCFGLDGYGRIDFGDTGPAGQKQTPFNTML-HQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFN INVGG+ N+VPFTAIFDSGTSFTYLTEP YSTI++QMDAGMKLKR+S FGP+F
Sbjct: 301 YNVTFNEINVGGKTNNVPFTAIFDSGTSFTYLTEPTYSTISEQMDAGMKLKRFS-FGPSF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PF+YCYEI P AKE +   LNFTM+GGDEF P DIFV +PVDET   ACLA+AKSTDIDL
Sbjct: 361 PFQYCYEISPSAKEIRRPQLNFTMEGGDEFVPIDIFVVIPVDETKSAACLALAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP-SDSPPTDSPPSDSP 498
           IGQNFMTGYRI FNR +MVLGWS SDCYDNG  TPS D+PPADSP SDSPPTDSPPSDSP
Sbjct: 421 IGQNFMTGYRIIFNRGEMVLGWSPSDCYDNGDSTPS-DSPPADSPPSDSPPTDSPPSDSP 480

Query: 499 PTD 500
           PTD
Sbjct: 481 PTD 480

BLAST of Cucsat.G1918 vs. NCBI nr
Match: XP_038882816.1 (aspartyl protease family protein 1-like [Benincasa hispida])

HSP 1 Score: 793 bits (2049), Expect = 3.29e-285
Identity = 399/492 (81.10%), Postives = 440/492 (89.43%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MA TF+SGAQMLLVLS+F+LAG LRSGDA+SFKF IHHRFSDS+KGI HSEGLPEKHTPG
Sbjct: 1   MAWTFTSGAQMLLVLSIFLLAGGLRSGDASSFKFSIHHRFSDSVKGILHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDR VRGRRLA    DTQLTFAYGNDT FIP+LGFLYYANVSVGTPSLDF VA
Sbjct: 61  YYATMVHRDRFVRGRRLAD---DTQLTFAYGNDTFFIPELGFLYYANVSVGTPSLDFFVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTS 198
           LDTGSDLFWLPCEC SCFTYLNTS+GG+FMLNHYSP+DSTTSSTVPC+SSLC   N+CTS
Sbjct: 121 LDTGSDLFWLPCECRSCFTYLNTSDGGQFMLNHYSPSDSTTSSTVPCSSSLCELSNQCTS 180

Query: 199 NQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAA 258
           NQN CPYE+ YLSANTSS+GYLVEDVLHLATDDSLLKPVEAKITFGCG VQTGIFA +AA
Sbjct: 181 NQNTCPYEINYLSANTSSVGYLVEDVLHLATDDSLLKPVEAKITFGCGKVQTGIFARSAA 240

Query: 259 PNGLIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLE 318
           PNGLIGLGMEKISVPSFLADQGLTS+SFSMCFG D YGRIDFGDTGPA QK+TPFN+ML 
Sbjct: 241 PNGLIGLGMEKISVPSFLADQGLTSDSFSMCFGLDNYGRIDFGDTGPAGQKETPFNSMLH 300

Query: 319 YQSYNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFG 378
           +QSYNV+FN I VG + N+VPFTAIFDSGTSFTYLTEPAYSTI++QMDAGM LKR++ F 
Sbjct: 301 FQSYNVSFNQIIVGDKTNNVPFTAIFDSGTSFTYLTEPAYSTISEQMDAGMDLKRFT-FD 360

Query: 379 PNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDET--THVACLAIAKS 438
            +FPFEYCYE+P  +K+ Q   LNFTMKGGD+F P D+F+ +P+D++  +  ACLAI KS
Sbjct: 361 SSFPFEYCYELPSNSKDVQRPLLNFTMKGGDDFVPLDLFIVVPIDDSGSSRAACLAILKS 420

Query: 439 TDIDLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP-SDSPPTDSP 498
           TDIDLIGQNFMTGYRI FNR++MVLGWS SDCYDNG GTPSGDTPP+DSP SDSPP DSP
Sbjct: 421 TDIDLIGQNFMTGYRIIFNREKMVLGWSLSDCYDNGDGTPSGDTPPSDSPPSDSPPADSP 480

Query: 499 PSD-SPPTDSPP 503
           P+D SPP DSPP
Sbjct: 481 PTDDSPPADSPP 488

BLAST of Cucsat.G1918 vs. NCBI nr
Match: XP_023518254.1 (aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708 bits (1828), Expect = 1.01e-251
Identity = 360/493 (73.02%), Postives = 416/493 (84.38%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MA TFSSG QMLLVLSV++LA  LR+G+AASFKF+IHHRFS+SIKGI  SEGLPEKHTP 
Sbjct: 1   MAWTFSSGVQMLLVLSVYLLACGLRNGEAASFKFNIHHRFSESIKGILTSEGLPEKHTPA 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLA+S+ DT+LTFAYGN+T +I +LG+LYYAN+SVG+PSLDFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLASSNGDTKLTFAYGNETFYIENLGYLYYANISVGSPSLDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTS 198
           LDTGSDL WLPCEC SC TYLNT++GGKF LNHYSP+DSTTS+ VPC++SLC   N+CTS
Sbjct: 121 LDTGSDLLWLPCECRSCLTYLNTTDGGKFALNHYSPDDSTTSAPVPCSNSLCELSNQCTS 180

Query: 199 NQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAA 258
           N N CPYE+ YLSANTSS GYLV+DVLHLATDD  L PVEAKITFGCGTVQTG+F   AA
Sbjct: 181 NTNTCPYEINYLSANTSSTGYLVQDVLHLATDDLKLDPVEAKITFGCGTVQTGVFQRGAA 240

Query: 259 PNGLIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLE 318
           PNGLIGLGM++ISVPS LA+QGL+++SFSMCFG DG GRIDFGD+G   Q++TPFNTM  
Sbjct: 241 PNGLIGLGMDRISVPSLLANQGLSTDSFSMCFGIDGIGRIDFGDSGTPGQRETPFNTMAN 300

Query: 319 YQSYNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFG 378
           Y SYNVT   I VGG+ N+V FTAIFDSGTSFTYL EPAYS I+KQM+AGMKLKR++   
Sbjct: 301 YPSYNVTVTEIIVGGKANNVEFTAIFDSGTSFTYLNEPAYSIISKQMNAGMKLKRFTT-D 360

Query: 379 PNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTD 438
           P+FPFEYCYE+P   K  + + LNFTM GGD+F P D+F+  P+D+TTH  CL + KS D
Sbjct: 361 PDFPFEYCYELPANDKVERPI-LNFTMMGGDDFVPLDLFIGFPIDDTTHAVCLTLIKSND 420

Query: 439 IDLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP--SDSPPTD-SP 498
           I+LIGQNFMTGYRI F+R++MVLGWS SDCYD+  GTPSGDTPPADSP   DSPP D SP
Sbjct: 421 INLIGQNFMTGYRIIFDREKMVLGWSPSDCYDSDAGTPSGDTPPADSPPAKDSPPADDSP 480

Query: 499 PS-DSPPT-DSPP 503
           P+ DSPP  DSPP
Sbjct: 481 PAEDSPPAEDSPP 491

BLAST of Cucsat.G1918 vs. ExPASy TrEMBL
Match: A0A0A0KML0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507250 PE=3 SV=1)

HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 485/486 (99.79%), Postives = 485/486 (99.79%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN
Sbjct: 121 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
           VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF
Sbjct: 301 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL
Sbjct: 361 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSDSPP 498
           IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPS SPP
Sbjct: 421 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSVSPP 480

Query: 499 TDSPPS 504
           TDSPPS
Sbjct: 481 TDSPPS 486

BLAST of Cucsat.G1918 vs. ExPASy TrEMBL
Match: A0A5A7SKI7 (Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00960 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 2.55e-301
Identity = 418/483 (86.54%), Postives = 447/483 (92.55%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLL+LSVFILAGSLRSGDAASFKF+IHHRFSDS+K + HSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLLLSVFILAGSLRSGDAASFKFNIHHRFSDSVKEVLHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAA++ DTQLTFAYGNDT FI +LGFLYYANVSVGTPS+DFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAATNDDTQLTFAYGNDTVFIAELGFLYYANVSVGTPSVDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSC TYLNTSNGGKFMLNHYSPNDSTTSS VPCTSSLC +CTSN+N
Sbjct: 121 LDTGSDLFWLPCECSSCATYLNTSNGGKFMLNHYSPNDSTTSSIVPCTSSLCGQCTSNKN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
           +CPYE+ Y+SANTSSIGYLVEDVLHLA DD+LLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 ICPYEINYVSANTSSIGYLVEDVLHLAADDALLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTS+SFSMCFG DGYGRIDFGDTGPA QKQTPFNTML +QS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSDSFSMCFGLDGYGRIDFGDTGPAGQKQTPFNTML-HQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFN INVGG+ N+VPFTAIFDSGTSFTYLTEP YSTI++QMDAGMKLKR+S FGP+F
Sbjct: 301 YNVTFNEINVGGKTNNVPFTAIFDSGTSFTYLTEPTYSTISEQMDAGMKLKRFS-FGPSF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PF+YCYEI P AKE +   LNFTM+GGDEF P DIFV +PVDET   ACLA+AKSTDIDL
Sbjct: 361 PFQYCYEISPSAKEIRRPQLNFTMEGGDEFVPIDIFVVIPVDETKSAACLALAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP-SDSPPTDSPPSDSP 498
           IGQNFMTGYRI FNR +MVLGWS SDCYDNG  TPS D+PPADSP SDSPPTDSPPSDSP
Sbjct: 421 IGQNFMTGYRIIFNRGEMVLGWSPSDCYDNGDSTPS-DSPPADSPPSDSPPTDSPPSDSP 480

Query: 499 PTD 500
           PTD
Sbjct: 481 PTD 480

BLAST of Cucsat.G1918 vs. ExPASy TrEMBL
Match: A0A1S4DT41 (aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485161 PE=3 SV=1)

HSP 1 Score: 832 bits (2150), Expect = 3.74e-301
Identity = 418/483 (86.54%), Postives = 446/483 (92.34%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MASTFSSGAQMLL+LSVFILAGSLRSGDAASFKF+IHHRFSDS+K + HSEGLPEKHTPG
Sbjct: 1   MASTFSSGAQMLLLLSVFILAGSLRSGDAASFKFNIHHRFSDSVKEVLHSEGLPEKHTPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLVRGRRLAA++ DTQLTFAYGNDT FI +LGFLYYANVSVGTPS+DFLVA
Sbjct: 61  YYATMVHRDRLVRGRRLAATNDDTQLTFAYGNDTVFIAELGFLYYANVSVGTPSVDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLCNRCTSNQN 198
           LDTGSDLFWLPCECSSC TYLNTSNGGKFMLNHYSPNDSTTSS VPCTSSLC +CTSN+N
Sbjct: 121 LDTGSDLFWLPCECSSCATYLNTSNGGKFMLNHYSPNDSTTSSIVPCTSSLCGQCTSNKN 180

Query: 199 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 258
            CPYE+ Y+SANTSSIGYLVEDVLHLA DD+LLKPVEAKITFGCGTVQTGIFATTAAPNG
Sbjct: 181 TCPYEINYVSANTSSIGYLVEDVLHLAADDALLKPVEAKITFGCGTVQTGIFATTAAPNG 240

Query: 259 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 318
           LIGLGMEKISVPSFLADQGLTS+SFSMCFG DGYGRIDFGDTGPA QKQTPFNTML +QS
Sbjct: 241 LIGLGMEKISVPSFLADQGLTSDSFSMCFGLDGYGRIDFGDTGPAGQKQTPFNTML-HQS 300

Query: 319 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 378
           YNVTFN INVGG+ N+VPFTAIFDSGTSFTYLTEP YSTI++QMDAGMKLKR+S FGP+F
Sbjct: 301 YNVTFNEINVGGKTNNVPFTAIFDSGTSFTYLTEPTYSTISEQMDAGMKLKRFS-FGPSF 360

Query: 379 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 438
           PF+YCYEI P AKE +   LNFTM+GGDEF P DIFV +PVDET   ACLA+AKSTDIDL
Sbjct: 361 PFQYCYEISPSAKEIRRPQLNFTMEGGDEFVPIDIFVVIPVDETKSAACLALAKSTDIDL 420

Query: 439 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSP-SDSPPTDSPPSDSP 498
           IGQNFMTGYRI FNR +MVLGWS SDCYDNG  TPS D+PPADSP SDSPPTDSPPSDSP
Sbjct: 421 IGQNFMTGYRIIFNRGEMVLGWSPSDCYDNGDSTPS-DSPPADSPPSDSPPTDSPPSDSP 480

Query: 499 PTD 500
           PTD
Sbjct: 481 PTD 480

BLAST of Cucsat.G1918 vs. ExPASy TrEMBL
Match: A0A6J1BTF7 (aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111005407 PE=3 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 3.25e-250
Identity = 359/493 (72.82%), Postives = 410/493 (83.16%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MA TFSSGAQMLL LSVF+LAG LRSG+A SFKF IHHRFSDSIKGI  SEGLPEK +PG
Sbjct: 1   MAWTFSSGAQMLLFLSVFLLAGELRSGEAGSFKFSIHHRFSDSIKGILDSEGLPEKQSPG 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRDRLV GRRLA ++ D  LTF YGNDT  I +LGFLYYAN+SVGTP L FLVA
Sbjct: 61  YYATMVHRDRLVHGRRLATTNGDPPLTFVYGNDTFLIGNLGFLYYANISVGTPELSFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTS 198
           LDTGSDLFWLPCECSSC TYLNT+NGGKF LNHYSP DSTTS++VPC++SLC   N+C+S
Sbjct: 121 LDTGSDLFWLPCECSSCLTYLNTTNGGKFGLNHYSPKDSTTSTSVPCSNSLCELANQCSS 180

Query: 199 NQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAA 258
             + CPYE+ YLSANTSS GYLV+DVLHLATDD  LKPV+AKITFGCG +QTGIFA +AA
Sbjct: 181 TTSTCPYEINYLSANTSSSGYLVQDVLHLATDDKQLKPVDAKITFGCGKIQTGIFADSAA 240

Query: 259 PNGLIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLE 318
           PNGLIGLGMEKISVPSFLA QGLTS+SFSMCFG DG GRIDFGD G + Q++TPFNTM+ 
Sbjct: 241 PNGLIGLGMEKISVPSFLASQGLTSDSFSMCFGYDGQGRIDFGDIGTSGQRETPFNTMVN 300

Query: 319 YQSYNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFG 378
           + SYNVTF  I VGG+ N++ F+AIFDSGTSF+Y+T+P YS I +QMDAGMKL+R   F 
Sbjct: 301 FPSYNVTFTQIIVGGKSNNLQFSAIFDSGTSFSYITDPVYSLIAEQMDAGMKLERVK-FD 360

Query: 379 PNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVA-CLAIAKST 438
           P+FPFEYCY++PP A  F +  LNFTMKGGD +   D FV +P D +  +A CL I KST
Sbjct: 361 PDFPFEYCYQLPPNANSFSHPRLNFTMKGGDNYGTLDPFVVVPTDNSGGLAACLGIVKST 420

Query: 439 D-IDLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADS--PSDSPPTDS 498
           D IDLIGQNFMTGYRI FNR++MVLGW+ SDCYDNG  TPS ++PPAD+  PSDSPPTDS
Sbjct: 421 DPIDLIGQNFMTGYRIIFNREKMVLGWTESDCYDNGAATPSDNSPPADNSPPSDSPPTDS 480

Query: 499 -PPSDS-PPTDSP 502
            PP+DS PP+DSP
Sbjct: 481 SPPTDSSPPSDSP 492

BLAST of Cucsat.G1918 vs. ExPASy TrEMBL
Match: A0A6J1EEK3 (aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111433625 PE=3 SV=1)

HSP 1 Score: 702 bits (1813), Expect = 6.23e-250
Identity = 359/500 (71.80%), Postives = 415/500 (83.00%), Query Frame = 0

Query: 19  MASTFSSGAQMLLVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPG 78
           MA TFSSG QMLLVLSV++LA  LRSG+AASFKF+IHHRFS+SIKGI  SEGLPEKHTP 
Sbjct: 1   MAWTFSSGVQMLLVLSVYLLACGLRSGEAASFKFNIHHRFSESIKGILTSEGLPEKHTPA 60

Query: 79  YYATMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVA 138
           YYATMVHRD LVRGRRLA+S+ DT+LTFAYGN+T +I +LG+LYYAN+SVG+PSLDFLVA
Sbjct: 61  YYATMVHRDMLVRGRRLASSNGDTKLTFAYGNETFYIENLGYLYYANISVGSPSLDFLVA 120

Query: 139 LDTGSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTS 198
           LDTGSDL WLPCEC SC TYLNT++GGKF LNHYSP+DSTTS+ VPC++SLC   N+CTS
Sbjct: 121 LDTGSDLLWLPCECRSCLTYLNTTDGGKFALNHYSPDDSTTSAPVPCSNSLCELSNQCTS 180

Query: 199 NQNVCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAA 258
           N N CPYE+ YLSANTSS GYLV+DVLHLATDD  L PVE+KITFGCGTVQTG+F   AA
Sbjct: 181 NTNTCPYEINYLSANTSSTGYLVQDVLHLATDDLKLDPVESKITFGCGTVQTGVFQRGAA 240

Query: 259 PNGLIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLE 318
           PNGLIGLGM++ISVPS LA+QGLT++SFSMCFG DG GRIDFGD+G   Q++TPFNTM  
Sbjct: 241 PNGLIGLGMDRISVPSLLANQGLTTDSFSMCFGIDGIGRIDFGDSGTPGQRETPFNTMAN 300

Query: 319 YQSYNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFG 378
           Y SYNVT   I VGG+ N+V FTAIFDSGTSFTYL EPAYS I++QM+AGMKLKR++   
Sbjct: 301 YPSYNVTVTEIIVGGKANNVEFTAIFDSGTSFTYLNEPAYSIISEQMNAGMKLKRFTT-D 360

Query: 379 PNFPFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTD 438
           P+FPFEYCYE+P   K  + + LNFTM GGD+F P D+F+  P+D+TTH  CL + KSTD
Sbjct: 361 PDFPFEYCYELPANDKVERPI-LNFTMMGGDDFVPMDLFIGFPIDDTTHAVCLTLIKSTD 420

Query: 439 IDLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPA-------DSP--SDS 498
           I+LIGQNFMTGYRI F+R++M LGWS SDCYD+  GTPSGDTPPA       DSP   DS
Sbjct: 421 INLIGQNFMTGYRIIFDREKMALGWSPSDCYDSDAGTPSGDTPPAKDSPPADDSPPAEDS 480

Query: 499 PPT-DSPPS-DSPPTDSPPS 504
           PP  DSPP+ DSPPTD  PS
Sbjct: 481 PPAEDSPPAEDSPPTDDSPS 498

BLAST of Cucsat.G1918 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 458.8 bits (1179), Expect = 5.8e-129
Identity = 245/488 (50.20%), Postives = 318/488 (65.16%), Query Frame = 0

Query: 25  SGAQMLLVLSVFILAGSL---RSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPGYYA 84
           S  ++L +  + +LA S    R      F F+ HHRFSD + G+   +GLP + +  YY 
Sbjct: 5   SSCRILFLGLLILLASSWVLDRCEGFGEFGFEFHHRFSDQVVGVLPGDGLPNRDSSKYYR 64

Query: 85  TMVHRDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDT 144
            M HRDRL+RGRRLA  D  + +TF+ GN+T  +  LGFL+YANV+VGTPS  F+VALDT
Sbjct: 65  VMAHRDRLIRGRRLANED-QSLVTFSDGNETVRVDALGFLHYANVTVGTPSDWFMVALDT 124

Query: 145 GSDLFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTSNQN 204
           GSDLFWLPC+C++C   L    G    LN YSPN S+TS+ VPC S+LC   +RC S ++
Sbjct: 125 GSDLFWLPCDCTNCVRELKAPGGSSLDLNIYSPNASSTSTKVPCNSTLCTRGDRCASPES 184

Query: 205 VCPYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNG 264
            CPY++RYLS  TSS G LVEDVLHL ++D   K + A++TFGCG VQTG+F   AAPNG
Sbjct: 185 DCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKSSKAIPARVTFGCGQVQTGVFHDGAAPNG 244

Query: 265 LIGLGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQS 324
           L GLG+E ISVPS LA +G+ +NSFSMCFG DG GRI FGD G  DQ++TP N    + +
Sbjct: 245 LFGLGLEDISVPSVLAKEGIAANSFSMCFGNDGAGRISFGDKGSVDQRETPLNIRQPHPT 304

Query: 325 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 384
           YN+T   I+VGG   D+ F A+FDSGTSFTYLT+ AY+ I++  ++    KRY       
Sbjct: 305 YNITVTKISVGGNTGDLEFDAVFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSEL 364

Query: 385 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDL 444
           PFEYCY + P    FQY  +N TMKGG  +      V +P+ + T V CLAI K  DI +
Sbjct: 365 PFEYCYALSPNKDSFQYPAVNLTMKGGSSYPVYHPLVVIPMKD-TDVYCLAIMKIEDISI 424

Query: 445 IGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDS--PPTDS--PPS 503
           IGQNFMTGYR+ F+R++++LGW  SDCY    G  S  T P++  S S  PP  S  P +
Sbjct: 425 IGQNFMTGYRVVFDREKLILGWKESDCY---TGETSARTLPSNRSSSSARPPASSFDPEA 484

BLAST of Cucsat.G1918 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.6e-113
Identity = 211/446 (47.31%), Postives = 288/446 (64.57%), Query Frame = 0

Query: 31  LVLSVFILAGSLRSGDAASFKFDIHHRFSDSIKGIFHSEG----LPEKHTPGYYATMVHR 90
           L L   ++  S  S +   F F++HHRFSD +K    S G     P K +  Y+  +V R
Sbjct: 10  LFLIPILMLLSFGSCNGRIFTFEMHHRFSDEVKQWSDSTGRFAKFPPKGSFEYFNALVLR 69

Query: 91  DRLVRGRRLAASDVDTQ--LTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSD 150
           D L+RGRRL+ S+ +++  LTF+ GN T+ I  LGFL+Y  V +GTP + F+VALDTGSD
Sbjct: 70  DWLIRGRRLSESESESESSLTFSDGNSTSRISSLGFLHYTTVKLGTPGMRFMVALDTGSD 129

Query: 151 LFWLPCECSSCFTYLNTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTSNQNVCP 210
           LFW+PC+C  C      +   +F L+ Y+P  STT+  V C +SLC   N+C    + CP
Sbjct: 130 LFWVPCDCGKCAPTEGATYASEFELSIYNPKVSTTNKKVTCNNSLCAQRNQCLGTFSTCP 189

Query: 211 YEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNGLIG 270
           Y + Y+SA TS+ G L+EDV+HL T+D   + VEA +TFGCG VQ+G F   AAPNGL G
Sbjct: 190 YMVSYVSAQTSTSGILMEDVMHLTTEDKNPERVEAYVTFGCGQVQSGSFLDIAAPNGLFG 249

Query: 271 LGMEKISVPSFLADQGLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQSYNV 330
           LGMEKISVPS LA +GL ++SFSMCFG DG GRI FGD G +DQ++TPFN    + +YN+
Sbjct: 250 LGMEKISVPSVLAREGLVADSFSMCFGHDGVGRISFGDKGSSDQEETPFNLNPSHPNYNI 309

Query: 331 TFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFE 390
           T   + VG    D  FTA+FD+GTSFTYL +P Y+T+++   +  + KR+S      PFE
Sbjct: 310 TVTRVRVGTTLIDDEFTALFDTGTSFTYLVDPMYTTVSESFHSQAQDKRHSP-DSRIPFE 369

Query: 391 YCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKSTDIDLIGQ 450
           YCY++   A      +L+ TMKG   FT  D  + +   E   V CLAI KS+++++IGQ
Sbjct: 370 YCYDMSNDANASLIPSLSLTMKGNSHFTINDPIIVIST-EGELVYCLAIVKSSELNIIGQ 429

Query: 451 NFMTGYRITFNRDQMVLGWSSSDCYD 468
           N+MTGYR+ F+R+++VL W   DCYD
Sbjct: 430 NYMTGYRVVFDREKLVLAWKKFDCYD 453

BLAST of Cucsat.G1918 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.7e-102
Identity = 219/489 (44.79%), Postives = 302/489 (61.76%), Query Frame = 0

Query: 28  QMLLVLSVFILAGSLRSGDAA-SFKFDIHHRFSDSIKGIFHSEGL-PEKHTPGYYATMVH 87
           Q+ ++LS+ ++   L   +A+  F F++HH FSD +K     + L PEK +  Y+  +  
Sbjct: 6   QVFVLLSLLVVCWGLERCEASGKFSFEVHHMFSDRVKQSLGLDDLVPEKGSLEYFKVLAQ 65

Query: 88  RDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSDL 147
           RDRL+RGR LA+++ +T +TF  GN T  I  LGFL+YANVSVGTP+  FLVALDTGSDL
Sbjct: 66  RDRLIRGRGLASNNEETPITFMRGNRTISIDLLGFLHYANVSVGTPATWFLVALDTGSDL 125

Query: 148 FWLPCEC-SSCFTYLNTSNGGKFM-LNHYSPNDSTTSSTVPCTSSLC---NRCTSNQNVC 207
           FWLPC C S+C   L      +   LN YSPN S+TSS++ C+   C   +RC+S  + C
Sbjct: 126 FWLPCNCGSTCIRDLKEVGLSQSRPLNLYSPNTSSTSSSIRCSDDRCFGSSRCSSPASSC 185

Query: 208 PYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNGLI 267
           PY+++YLS +T + G L EDVLHL T+D  L+PV+A IT GCG  QTG   ++AA NGL+
Sbjct: 186 PYQIQYLSKDTFTTGTLFEDVLHLVTEDEGLEPVKANITLGCGKNQTGFLQSSAAVNGLL 245

Query: 268 GLGMEKISVPSFLADQGLTSNSFSMCFG--ADGYGRIDFGDTGPADQKQTPFNTMLEYQS 327
           GLG++  SVPS LA   +T+NSFSMCFG   D  GRI FGD G  DQ +TP        +
Sbjct: 246 GLGLKDYSVPSILAKAKITANSFSMCFGNIIDVVGRISFGDKGYTDQMETPLLPTEPSPT 305

Query: 328 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 387
           Y V+   ++VGG+   V   A+FD+GTSFT+L EP Y  ITK  D  +  KR  +  P  
Sbjct: 306 YAVSVTEVSVGGDAVGVQLLALFDTGTSFTHLLEPEYGLITKAFDDHVTDKRRPI-DPEL 365

Query: 388 PFEYCYEIPPGAKEFQYLTLNFTMKGGDE-FTPTDIFVFLPVDETTHVACLAIAKSTD-- 447
           PFE+CY++ P      +  +  T +GG + F    +F+    D +  + CL I KS D  
Sbjct: 366 PFEFCYDLSPNKTTILFPRVAMTFEGGSQMFLRNPLFIVWNEDNSA-MYCLGILKSVDFK 425

Query: 448 IDLIGQNFMTGYRITFNRDQMVLGWSSSDCY-DNGVGTPSGDTPPADSPSDSPPTDSPPS 504
           I++IGQNFM+GYRI F+R++M+LGW  SDC+ D  + + +   P  ++PS S  T  P  
Sbjct: 426 INIIGQNFMSGYRIVFDRERMILGWKRSDCFEDESLESTTPPPPETEAPSPSASTPLPSL 485

BLAST of Cucsat.G1918 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-99
Identity = 211/490 (43.06%), Postives = 303/490 (61.84%), Query Frame = 0

Query: 28  QMLLVLSVFILA-GSLRSGDAASFKFDIHHRFSDSIK-GIFHSEGLPEKHTPGYYATMVH 87
           Q+ ++LSV ++  G  R      F F++HH FSDS+K  +   + +PE+ +  Y+  + H
Sbjct: 6   QVFVLLSVLVVCWGFERCEATGKFGFEVHHIFSDSVKQSLGLGDLVPEQGSLEYFKVLAH 65

Query: 88  RDRLVRGRRLAASDVDTQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSDL 147
           RDRL+RGR LA+++ +T +TF  GN T  +  LG LYYANVSVGTP   FLVALDTGSDL
Sbjct: 66  RDRLIRGRGLASNNDETPITFDGGNLTVSVKLLGSLYYANVSVGTPPSSFLVALDTGSDL 125

Query: 148 FWLPCEC-SSCFTYLNTSNGGKFM-LNHYSPNDSTTSSTVPCTSSLC---NRCTSNQNVC 207
           FWLPC C ++C   L      + + LN Y+PN STTSS++ C+   C    +C+S  ++C
Sbjct: 126 FWLPCNCGTTCIRDLEDIGVPQSVPLNLYTPNASTTSSSIRCSDKRCFGSKKCSSPSSIC 185

Query: 208 PYEMRYLSANTSSIGYLVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNGLI 267
           PY++ Y S +T + G L++DVLHLAT+D  L PV+A +T GCG  QTG+F    + NG++
Sbjct: 186 PYQISY-SNSTGTKGTLLQDVLHLATEDENLTPVKANVTLGCGQKQTGLFQRNNSVNGVL 245

Query: 268 GLGMEKISVPSFLADQGLTSNSFSMCFG--ADGYGRIDFGDTGPADQKQTPFNTMLEYQS 327
           GLG++  SVPS LA   +T+NSFSMCFG      GRI FGD G  DQ++TPF ++    +
Sbjct: 246 GLGIKGYSVPSLLAKANITANSFSMCFGRVIGNVGRISFGDRGYTDQEETPFISVAPSTA 305

Query: 328 YNVTFNVINVGGEPNDVPFTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNF 387
           Y V  + ++V G+P D+   A FD+G+SFT+L EPAY  +TK  D  ++ +R  +  P  
Sbjct: 306 YGVNISGVSVAGDPVDIRLFAKFDTGSSFTHLREPAYGVLTKSFDELVEDRRRPV-DPEL 365

Query: 388 PFEYCYEIPPGAKEFQYLTLNFTMKGGDEFTPTDIFVFLPVDETTHVACLAIAKST--DI 447
           PFE+CY++ P A   Q+  +  T  GG +    + F      E   + CL + KS    I
Sbjct: 366 PFEFCYDLSPNATTIQFPLVEMTFIGGSKIILNNPFFTARTQEGNVMYCLGVLKSVGLKI 425

Query: 448 DLIGQNFMTGYRITFNRDQMVLGWSSSDCYDNGVGTPSGDTPPADSPSDSPPTDSPPSDS 504
           ++IGQNF+ GYRI F+R++M+LGW  S C+++     S   PP +  + +P   +PP  S
Sbjct: 426 NVIGQNFVAGYRIVFDRERMILGWKQSLCFED-ESLESTTPPPPEVEAPAPSVSAPPPRS 485

BLAST of Cucsat.G1918 vs. TAIR 10
Match: AT3G51360.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.6e-97
Identity = 203/460 (44.13%), Postives = 283/460 (61.52%), Query Frame = 0

Query: 42  LRSGDAASFKFDIHHRFSDSIKGIFHSEGLPEKHTPGYYATMVHRDRLVRGRRLAASDVD 101
           L S  + S  F+IHHRFS+ +K +    GLPE  +  YY  +VHRD   RGR+L +++ +
Sbjct: 14  LASSVSGSLSFEIHHRFSEQVKTVLGGHGLPEMGSLDYYKALVHRD---RGRQLTSNNNN 73

Query: 102 -TQLTFAYGNDTAFIPDLGFLYYANVSVGTPSLDFLVALDTGSDLFWLPCEC-SSCFTYL 161
            T ++FA GN T    ++ FL+YANV++GTP+  FLVALDTGSDLFWLPC C S+C   +
Sbjct: 74  QTTISFAQGNST---EEISFLHYANVTIGTPAQWFLVALDTGSDLFWLPCNCNSTCVRSM 133

Query: 162 NTSNGGKFMLNHYSPNDSTTSSTVPCTSSLC---NRCTSNQNVCPYEMRYLSANTSSIGY 221
            T  G +  LN Y+P+ S +SS V C S+LC   NRC S  + CPY +RYLS  + S G 
Sbjct: 134 ETDQGERIKLNIYNPSKSKSSSKVTCNSTLCALRNRCISPVSDCPYRIRYLSPGSKSTGV 193

Query: 222 LVEDVLHLATDDSLLKPVEAKITFGCGTVQTGIFATTAAPNGLIGLGMEKISVPSFLADQ 281
           LVEDV+H++T++   +  +A+ITFGC   Q G+F   A  NG++GL +  I+VP+ L   
Sbjct: 194 LVEDVIHMSTEEGEAR--DARITFGCSESQLGLFKEVAV-NGIMGLAIADIAVPNMLVKA 253

Query: 282 GLTSNSFSMCFGADGYGRIDFGDTGPADQKQTPFNTMLEYQSYNVTFNVINVGGEPNDVP 341
           G+ S+SFSMCFG +G G I FGD G +DQ +TP +  +    Y+V+     VG    D  
Sbjct: 254 GVASDSFSMCFGPNGKGTISFGDKGSSDQLETPLSGTISPMFYDVSITKFKVGKVTVDTE 313

Query: 342 FTAIFDSGTSFTYLTEPAYSTITKQMDAGMKLKRYSLFGPNFPFEYCYEIPPGAKEFQYL 401
           FTA FDSGT+ T+L EP Y+ +T      +  +R S    + PFE+CY I   + E +  
Sbjct: 314 FTATFDSGTAVTWLIEPYYTALTTNFHLSVPDRRLSK-SVDSPFEFCYIITSTSDEDKLP 373

Query: 402 TLNFTMKGG---DEFTPTDIFVFLPVDETTHVACLAIAK--STDIDLIGQNFMTGYRITF 461
           +++F MKGG   D F+P  I VF   D +  V CLA+ K  + D  +IGQNFMT YRI  
Sbjct: 374 SVSFEMKGGAAYDVFSP--ILVFDTSDGSFQVYCLAVLKQVNADFSIIGQNFMTNYRIVH 433

Query: 462 NRDQMVLGWSSSDCYD-NGVGTPSG-DTPPADSPSDSPPT 490
           +R++ +LGW  S+C D NG   P+    PP+ +P+ SP T
Sbjct: 434 DRERRILGWKKSNCNDTNGFTGPTALAKPPSMAPTSSPRT 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYV98.2e-12850.20Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX209.8e-6537.53Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q4V3D23.8e-2424.15Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9S9K41.9e-2325.28Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q766C35.5e-2325.96Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_031742572.10.099.79aspartyl protease family protein 1 [Cucumis sativus] >KGN48951.1 hypothetical pr... [more]
KAA0025642.15.26e-30186.54aspartyl protease family protein 1-like [Cucumis melo var. makuwa] >TYK12515.1 a... [more]
XP_016899154.17.72e-30186.54PREDICTED: aspartyl protease family protein 1-like [Cucumis melo][more]
XP_038882816.13.29e-28581.10aspartyl protease family protein 1-like [Benincasa hispida][more]
XP_023518254.11.01e-25173.02aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KML00.099.79Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G50725... [more]
A0A5A7SKI72.55e-30186.54Aspartyl protease family protein 1-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S4DT413.74e-30186.54aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485161 ... [more]
A0A6J1BTF73.25e-25072.82aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1EEK36.23e-25071.80aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G17760.15.8e-12950.20Eukaryotic aspartyl protease family protein [more]
AT4G35880.12.6e-11347.31Eukaryotic aspartyl protease family protein [more]
AT3G51330.12.7e-10244.79Eukaryotic aspartyl protease family protein [more]
AT3G51350.12.1e-9943.06Eukaryotic aspartyl protease family protein [more]
AT3G51360.17.6e-9744.13Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 339..350
score: 47.29
coord: 128..148
score: 44.59
coord: 437..452
score: 23.01
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 25..476
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 319..461
e-value: 8.4E-12
score: 45.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 111..299
e-value: 2.1E-41
score: 144.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 303..466
e-value: 6.0E-27
score: 96.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 117..466
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 122..299
e-value: 6.8E-34
score: 117.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 468..504
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 480..504
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 25..476
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 137..148
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 339..350
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 122..461
score: 34.188499

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G1918.T1Cucsat.G1918.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity