Cucsat.G1865 (gene) Cucumber (B10) v3

BLAST of Cucsat.G1865 vs. ExPASy Swiss-Prot
Match: A2RVJ8 (Protein HEAT INTOLERANT 4 OS=Arabidopsis thaliana OX=3702 GN=HIT4 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.8e-29
Identity = 70/119 (58.82%), Postives = 89/119 (74.79%), Query Frame = 0

Query: 8   FCNPLKKP--EQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+   EQSTEVQI+FP+EP P+ CEFDW+  +LQE  D L+EEE L  +Q D FK
Sbjct: 302 FYQPFKEDELEQSTEVQIMFPSEP-PVVCEFDWEFDELQEFVDKLVEEEALPAEQADEFK 361

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 125
           E+VKE+VR A KANRE ++AR KA  EMSE+ K AF+KM+FYKFYP  + D+P+VS V+
Sbjct: 362 EYVKEQVRAAKKANREAKDARKKAIEEMSEDTKQAFQKMKFYKFYPQPSPDTPDVSGVQ 419

BLAST of Cucsat.G1865 vs. NCBI nr
Match: KAE8647582.1 (hypothetical protein Csa_003773 [Cucumis sativus])

HSP 1 Score: 240 bits (613), Expect = 4.80e-76
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0

Query: 1   MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ 60
           MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ
Sbjct: 217 MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ 276

Query: 61  KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV 120
           KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV
Sbjct: 277 KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV 336

Query: 121 SQVK 124
           SQVK
Sbjct: 337 SQVK 340

BLAST of Cucsat.G1865 vs. NCBI nr
Match: XP_011658000.1 (protein HEAT INTOLERANT 4 [Cucumis sativus])

HSP 1 Score: 229 bits (583), Expect = 1.12e-71
Identity = 117/122 (95.90%), Postives = 120/122 (98.36%), Query Frame = 0

Query: 3   FSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKD 62
           + + +FCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKD
Sbjct: 206 YCLPHFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKD 265

Query: 63  AFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQ 122
           AFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQ
Sbjct: 266 AFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQ 325

Query: 123 VK 124
           VK
Sbjct: 326 VK 327

BLAST of Cucsat.G1865 vs. NCBI nr
Match: XP_022133379.1 (uncharacterized protein LOC111005966 [Momordica charantia])

HSP 1 Score: 171 bits (432), Expect = 6.33e-49
Identity = 94/119 (78.99%), Postives = 99/119 (83.19%), Query Frame = 0

Query: 8   FCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+ E  QSTEVQIIFPAEPKPIFCEFDW+  +LQE TD LIEEEELS DQKDAFK
Sbjct: 211 FYQPFKEDELEQSTEVQIIFPAEPKPIFCEFDWELDELQEFTDKLIEEEELSEDQKDAFK 270

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           EFVKEKVREA KANRE  EAR K   EMSEE KAAFEKMRFYKFYPVQTQDSP++S VK
Sbjct: 271 EFVKEKVREAKKANREAREARKKVLQEMSEETKAAFEKMRFYKFYPVQTQDSPDISNVK 329

BLAST of Cucsat.G1865 vs. NCBI nr
Match: XP_041007165.1 (LOW QUALITY PROTEIN: protein HEAT INTOLERANT 4-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 166 bits (420), Expect = 3.68e-47
Identity = 91/116 (78.45%), Postives = 98/116 (84.48%), Query Frame = 0

Query: 11  PLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFKEFV 70
           PLK+ E  QSTEVQIIFPAE KP+FCEFDW+  +L+E TD LI+EEELS DQKDAFKEFV
Sbjct: 211 PLKEDELEQSTEVQIIFPAEQKPVFCEFDWELDELEEFTDKLIQEEELSEDQKDAFKEFV 270

Query: 71  KEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           KEKVREA KANRE  EAR KAC EMSEE KAAFE MRFYKFYPVQT DSP+VS VK
Sbjct: 271 KEKVREAKKANREAREARKKACEEMSEETKAAFENMRFYKFYPVQTPDSPDVSNVK 326

BLAST of Cucsat.G1865 vs. NCBI nr
Match: XP_030949647.1 (protein HEAT INTOLERANT 4-like, partial [Quercus lobata])

HSP 1 Score: 162 bits (409), Expect = 8.24e-47
Identity = 89/119 (74.79%), Postives = 97/119 (81.51%), Query Frame = 0

Query: 8   FCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+ E  QSTEVQIIFP EPKPIFCEFDW+  +L+E TD LI+EEEL+ DQKDAFK
Sbjct: 96  FYQPFKEDELEQSTEVQIIFPVEPKPIFCEFDWELDELEEFTDKLIQEEELAEDQKDAFK 155

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           EFVKEKVREA KANRE  EAR KA  EM+EE KAAFE MRFYKFYPVQT DSP+VS VK
Sbjct: 156 EFVKEKVREAKKANREAREARKKALEEMTEESKAAFENMRFYKFYPVQTPDSPDVSSVK 214

BLAST of Cucsat.G1865 vs. ExPASy TrEMBL
Match: A0A0A0KK99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501910 PE=4 SV=1)

HSP 1 Score: 240 bits (613), Expect = 2.27e-79
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0

Query: 1   MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ 60
           MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ
Sbjct: 1   MLFSVANFCNPLKKPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQ 60

Query: 61  KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV 120
           KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV
Sbjct: 61  KDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNV 120

Query: 121 SQVK 124
           SQVK
Sbjct: 121 SQVK 124

BLAST of Cucsat.G1865 vs. ExPASy TrEMBL
Match: A0A6J1BVU0 (uncharacterized protein LOC111005966 OS=Momordica charantia OX=3673 GN=LOC111005966 PE=4 SV=1)

HSP 1 Score: 171 bits (432), Expect = 3.07e-49
Identity = 94/119 (78.99%), Postives = 99/119 (83.19%), Query Frame = 0

Query: 8   FCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+ E  QSTEVQIIFPAEPKPIFCEFDW+  +LQE TD LIEEEELS DQKDAFK
Sbjct: 211 FYQPFKEDELEQSTEVQIIFPAEPKPIFCEFDWELDELQEFTDKLIEEEELSEDQKDAFK 270

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           EFVKEKVREA KANRE  EAR K   EMSEE KAAFEKMRFYKFYPVQTQDSP++S VK
Sbjct: 271 EFVKEKVREAKKANREAREARKKVLQEMSEETKAAFEKMRFYKFYPVQTQDSPDISNVK 329

BLAST of Cucsat.G1865 vs. ExPASy TrEMBL
Match: A0A7N2N9J2 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 162 bits (409), Expect = 5.81e-48
Identity = 89/119 (74.79%), Postives = 97/119 (81.51%), Query Frame = 0

Query: 8   FCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+ E  QSTEVQIIFP EPKPIFCEFDW+  +L+E TD LI+EEEL+ DQKDAFK
Sbjct: 38  FYQPFKEDELEQSTEVQIIFPVEPKPIFCEFDWELDELEEFTDKLIQEEELAEDQKDAFK 97

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           EFVKEKVREA KANRE  EAR KA  EM+EE KAAFE MRFYKFYPVQT DSP+VS VK
Sbjct: 98  EFVKEKVREAKKANREAREARKKALEEMTEESKAAFENMRFYKFYPVQTPDSPDVSSVK 156

BLAST of Cucsat.G1865 vs. ExPASy TrEMBL
Match: A0A151TKL1 (Myosin-H heavy chain OS=Cajanus cajan OX=3821 GN=KK1_023919 PE=4 SV=1)

HSP 1 Score: 165 bits (417), Expect = 6.07e-47
Identity = 91/122 (74.59%), Postives = 100/122 (81.97%), Query Frame = 0

Query: 6   ANFCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDA 65
           A+F  P K+ E  QSTEV IIFPAEPKP+FCEFDW+  +L+E TD LIEEEELS DQKDA
Sbjct: 223 ADFYQPFKEDEIEQSTEVPIIFPAEPKPVFCEFDWELDELEEFTDKLIEEEELSEDQKDA 282

Query: 66  FKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQV 125
           FKEFVKEKVREA KANRE  EAR KA  EMSEE KAAFE MRFYKFYPVQ+ D+P+VS V
Sbjct: 283 FKEFVKEKVREAKKANREAREARKKAIEEMSEETKAAFESMRFYKFYPVQSPDAPDVSNV 342

BLAST of Cucsat.G1865 vs. ExPASy TrEMBL
Match: C6TH18 (Uncharacterized protein OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 159 bits (402), Expect = 1.48e-46
Identity = 87/119 (73.11%), Postives = 97/119 (81.51%), Query Frame = 0

Query: 8   FCNPLKKPE--QSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F +P K+ E  QSTEVQIIFPAEPKP+FCEFDW+  +L+E TD LIEEEEL  DQKD FK
Sbjct: 58  FYHPFKEDELEQSTEVQIIFPAEPKPVFCEFDWELDELEEFTDKLIEEEELLEDQKDTFK 117

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 124
           EFVKEKVREA KANRE  EAR KA  EMSEE +AAFE MRFYKFYPVQ+ D+P+VS VK
Sbjct: 118 EFVKEKVREAKKANREAREARRKAIQEMSEEARAAFENMRFYKFYPVQSPDAPDVSNVK 176

BLAST of Cucsat.G1865 vs. TAIR 10
Match: AT5G10010.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G64910.1); Has 33260 Blast hits to 16857 proteins in 1270 species: Archae - 88; Bacteria - 3040; Metazoa - 11915; Fungi - 3137; Plants - 1371; Viruses - 424; Other Eukaryotes - 13285 (source: NCBI BLink). )

HSP 1 Score: 128.3 bits (321), Expect = 5.5e-30
Identity = 70/119 (58.82%), Postives = 89/119 (74.79%), Query Frame = 0

Query: 8   FCNPLKKP--EQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVDQKDAFK 67
           F  P K+   EQSTEVQI+FP+EP P+ CEFDW+  +LQE  D L+EEE L  +Q D FK
Sbjct: 302 FYQPFKEDELEQSTEVQIMFPSEP-PVVCEFDWEFDELQEFVDKLVEEEALPAEQADEFK 361

Query: 68  EFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPNVSQVK 125
           E+VKE+VR A KANRE ++AR KA  EMSE+ K AF+KM+FYKFYP  + D+P+VS V+
Sbjct: 362 EYVKEQVRAAKKANREAKDARKKAIEEMSEDTKQAFQKMKFYKFYPQPSPDTPDVSGVQ 419

BLAST of Cucsat.G1865 vs. TAIR 10
Match: AT5G64910.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 58/125 (46.40%), Postives = 83/125 (66.40%), Query Frame = 0

Query: 7   NFC-----NPLK--KPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVD 66
           N+C     NP K    E+ST V+I+FP+EP P+ CE+DW +  ++E TD LI EE L  +
Sbjct: 335 NYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECEYDWVKSVIEEFTDSLINEEVLLPE 394

Query: 67  QKDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPN 125
           QK AF+EFVKEK  +AM A    +EA  KA   +SEE K A+++MR YKFYP+ + D+P+
Sbjct: 395 QKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSEETKKAYQEMRLYKFYPLPSPDTPH 454

BLAST of Cucsat.G1865 vs. TAIR 10
Match: AT5G64910.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1). )

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 58/125 (46.40%), Postives = 83/125 (66.40%), Query Frame = 0

Query: 7   NFC-----NPLK--KPEQSTEVQIIFPAEPKPIFCEFDWKEHKLQELTDDLIEEEELSVD 66
           N+C     NP K    E+ST V+I+FP+EP P+ CE+DW +  ++E TD LI EE L  +
Sbjct: 332 NYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECEYDWVKSVIEEFTDSLINEEVLLPE 391

Query: 67  QKDAFKEFVKEKVREAMKANREEEEARVKACLEMSEERKAAFEKMRFYKFYPVQTQDSPN 125
           QK AF+EFVKEK  +AM A    +EA  KA   +SEE K A+++MR YKFYP+ + D+P+
Sbjct: 392 QKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSEETKKAYQEMRLYKFYPLPSPDTPH 451