Cucsat.G18523 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G18523
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	plant-specific TFIIB-related protein PTF2
Location	ctg3379: 890939 .. 895101 (+)
RNA-Seq Expression	Cucsat.G18523
Synteny	Cucsat.G18523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTGCAACGATTCCGGAAAGTCCAGTTCTTAGGGTTTGCTGCACTTTGCACAAAGTCCTGAAATTTTCTTCCCCGGCGTATAATTCATCGGTGAAATCTCCTAATTTGCTTGTAATTTTCTTATCTCTAGTGAATCTCAAGCTGATGCATTGAATTTCTTGCCTCCAATCTCGTCTCTCCGATGTCCGGCTCTTGTAAGAATTGCCACAGCAGGTCCATCTTCCGTGACGATGTTTCCGGAAACCAGATTTGCTCCTCCTGCGGAATTGTTCAGGAATTTGACAATTATGACGCTCAGCTTGGCGGTATCAATGGTCCACAGGGCACATTCGTCCGCGTTGGAACATCAGGTTCGGGCAGCGTTTTGAATTATAAGGACAAGAAGATCTATGAGGCTCAAAAAGTTATTGAAGATATTACGTTTAGGTTAGGATTTTCTGCTTCTAAATCCAATGATGTTAGGATTTTAGTATCGACGATTACAGAGGGCGAGTATGGATTAGGTGATTGGTTCCCAATTCTCGTTGGTGCTTGTGCGTACGTTTCTATGCGCAAGGATAGTCGGCCATTATCAATGTCAGAAGTTGCATCTGCCGTCGAATGTGATCTTCATGAACTTGGCAGAATGGTTATGAGAGTTGTTGAATTTTTGGATTTGAGAGGGTCTGAGTTTCCGGTGTTTGATATTGTGGGGTCATTAGAGCGGGCCGCTAGGAACTCACCTTCTTTCTCAAGATTAGAAGCTGATATTCTTGAGAGAATTGTTAAGCAAGGAATTTTTTTGCTACAATGTGCTATGAAATGGTTTCTGACAACTGGGCGGCAACCGCTACCAATGGTGGCTGCGGTGTTAGTTCTGGTGTCGAAATTGAATGAGGTTGATGTTAGTATTGAGAATGTAGGAATGGAGGTTCATGCGAACGTTTCAACATGTAAAAAGAGGTATAGGGAACTTCTGGAGGCACTTGTGGAAGTTGGAAAAAAGTTGCCTTGGGGAAAAGATATCACGACAAAGAATATTGTAAAGAATGCTCCTTTTGTGATTCAATATATGGAATTGAAATCAATGTCAAAGGCCAGTGGTAAGGGGAAGGACTTGGAGAATGTGGAGATTGATTTGCAGTCTGCAGTGAGTGAGTGTTTAAGAAAAGAGCTTGAGTATGAGAGTGAGGTTTATAATTTAGAAGACGATTCTCAGTATTTTGAGTTACAACGCAGTAGATGGCAGGATGAATCGAATCGTGATAATGGGAACAGGCTCAATATTTCACATGAATGTTTATCCTTAATTTATAATAAATTTTTAGATGAAATGGCTGAGCTCAGGTCGTCGGGTGGTATTAATGAGGTCTATGGTACAAAGCAGGGAAGAAAAACTGGTTTTTACTCAAGTACAGAGTGGTGGGAGGGGAAATCAGAATTAAGCAAAAAGCTTCTATTGCAGCAATTATTGGAGACTGATATAGGGTCGCAGGGTATCCCACCATCATTTGTTTCGAGTTGCAATGCATACGAAAGGAGGAAGGAGAAAGTAAACGCTGCTAAGAAGCGAATCCAGAGGATTATGCACCCTTCAACTGCTCCAGCTGATGATGTAAATATTAAGAAGAAAAGAAAGCGTAAGGGAGCTGATGTTATAGAATGGGAAGATATAATAATTGAAACCCTTCTTCTCCATGGAGTACAGGAGGAGGAAATTGAGAAGGGCCATTACAAGGTCTTGTTGGACTTGTACGTCTTCACTTCTTCAACTTTTTAGACGTTTTTTTTCCATATGGAGACAATACTGAAAGTGTATAGGAAGGTACAGGCAAAGATCTGAATATCTGAGAAATAAGATGTAGAATTTTCAGCACAAAGAGAAGTTATTTCTGGTTGATATTGTTGATTGATAAAGATTTTGGATGTTCATTTATGAATTCAGTCGGAACTTGAGTTAAAATTTGTAAACAATTCTTGGAATTCTGGCTGGCAATACAGATGATGAATTGACTAGAAAGCAACTCCTATTTGTTTCAAATTGTTGTCTTGTTTTGTTCTGGAACTACATAAAATGGTCTATTCGAATTACGTCAACTTCTGCTATTGCTCGTGGTTTACGTCAATGATTCAACTCTCTAGTAACTCTTGGTAGTTCAAAGGATTCTTCTAAAGGTAAATTTCTTCCTAACTTTTTTTTTTGTCATAACAGCAACTCGATTTAAAATACTGCTCTTGCAATGAATTTCTTTCACAATTGGATATAATATCAATGAAGACAAGAGAAGATTGAAGGGAAAAATGATGAAGATTTTTTTTGAAGACCTCTTTTTCTTTTTTAATTCTGTTAGAATCTATATATATGGAATAAATAATTATGCAAAACTTACCCTAAATATTTCTTTTTCTTTTCTTCTTTGCTCTTTACCTTCTATTTAAGTTCCCTCATTTGTACAATTGTATTCATTAGAAAAACAATAAAACTATAAACATCATGATTTTTCTCCCGGTTCTTTTTCCACGTAAACCTTGATTTCATGTTTATTGCTTTCAATATGGTATCATAGCGAAGCAACAACGAAACCCTAGAAAAAATTTTAGGAAACACCCGGACCAAAACAGAAGTTACCGTCGCCGCTGTGTCCACCACCATGGAAAAATTACTCCACCGAATTCAAGTGTAAATCTACATAGTGGGCCAACCCTTGCTGTCGTCTGCGCATTATTTCGACTAGAAAACATCGCACGCGCTGCTTCTGTCGGGCGTATTGGCCCATGCATTGTCGTCCATTCATCTCACTGCCCATCCCATCCGCCTCTACAAGCTGTCGCATATCCAACCATTTTACCCTTTCAGTTAGGGGTGTTTAAAAAACTCGATAATCCGCCCAACCCGAATTACCCAACCCAAATTGTAAGGGTTGGATTGGGTTAAATTAGGTTTTGAGTTGGGTTGGGTTAAATTAAGTTTTGGGTTGGGTTGGGTTAGAGATTGAAAAAAAATTAAAATGAGCAGGGTTACCAGGTTGGAGAATCAAGGGTCGTGTTTACCCAACCCGACCTGGTCATATATATATAAAACCCTAACTTGTCATTTTAATTTTTCTTTTCCCCACCACAAACGGCGCACAACCATGACATTTCATCCGTTCTTCAGTTCCAACTTCCATACTTCGTAGTTCTCCGGCCTTCAGACGTTTTAGCCAAGAATGTTATGAACTTCTCATTCACAAGTTGCACCTCGCAGGCCGCAGTCTCAAGCTTTGTTCCCAACATTTTCTGTTAGTCCGTTTCCCATCGCAGTTATCAATTTCGACCTTTGCACGTTTCGATCGACAAACGTTTTTGTCGCACTTCGCAAGCTGCACTCTCAAGCTTTGTTCCCAACATATTCCGTTCGTTTGTTTCCCTTCACAGTATCAATTCTGACCTTCGCACGTTTCGACTGGCGAACAGTTTAGCTTCTTTCGTTCCCTTCGCAATCGCAAGATCTAAGATCTAAGTTTCAGCCGACAAGCATAAATTACTTTTGTTATATTTTGTTGTCTTTAGAATAAATTCAATCTATTCTATTCTAAGTTTCTTAATCTTTTGGAGCCTTCCACGTTCAATATACCACATATTTGGGTTTCTTTAAAATTCGTCACCATGAAAATCACCCTTATCTAATTCTAGGGTAAGTTAAATATTTGATAGTTAACTTTGTCTTGTGTTTAATAATTTGATGCATATATTGTGAATTGGATTTTATATGTTTGTGAAACTTTGTTTTGTGTTTAATAATTTGATGCATATATTGTGAATTGGATTTTATATGTTTGTGAAGCTTTGTTTTGGGTTTAATAATTTGATGCATATTTTGTGAATTTGGATTTTATATGTTGTGAAACTCTCAATTGTGTTGCCAAGTTTATCAGCTGTGTTTAATAGTTATGAATTTAATTAGATCTTGGTTTATAATATGTTGTGAATTTGGTTTAGTTCTCAATTATGGTGTTATAAAATTTTGAAAAAACAATATGATTAAAAAAAGAAAAAAAAGGCCAACCCGTCAACCTAATGTGCCTTCGAAAGAACTTGGTTACTTTGGGTTGGTCCATATTTTTCTTCCAACTCGGTCCAACCCGGCTTATGTACACCTACTTTCGGTCATCCGCAGCCGCACGTGCCACCCATTGTCGTA

Coding sequence (CDS)

ATGTCCGGCTCTTGTAAGAATTGCCACAGCAGGTCCATCTTCCGTGACGATGTTTCCGGAAACCAGATTTGCTCCTCCTGCGGAATTGTTCAGGAATTTGACAATTATGACGCTCAGCTTGGCGGTATCAATGGTCCACAGGGCACATTCGTCCGCGTTGGAACATCAGGTTCGGGCAGCGTTTTGAATTATAAGGACAAGAAGATCTATGAGGCTCAAAAAGTTATTGAAGATATTACGTTTAGGTTAGGATTTTCTGCTTCTAAATCCAATGATGTTAGGATTTTAGTATCGACGATTACAGAGGGCGAGTATGGATTAGGTGATTGGTTCCCAATTCTCGTTGGTGCTTGTGCGTACGTTTCTATGCGCAAGGATAGTCGGCCATTATCAATGTCAGAAGTTGCATCTGCCGTCGAATGTGATCTTCATGAACTTGGCAGAATGGTTATGAGAGTTGTTGAATTTTTGGATTTGAGAGGGTCTGAGTTTCCGGTGTTTGATATTGTGGGGTCATTAGAGCGGGCCGCTAGGAACTCACCTTCTTTCTCAAGATTAGAAGCTGATATTCTTGAGAGAATTGTTAAGCAAGGAATTTTTTTGCTACAATGTGCTATGAAATGGTTTCTGACAACTGGGCGGCAACCGCTACCAATGGTGGCTGCGGTGTTAGTTCTGGTGTCGAAATTGAATGAGGTTGATGTTAGTATTGAGAATGTAGGAATGGAGGTTCATGCGAACGTTTCAACATGTAAAAAGAGGTATAGGGAACTTCTGGAGGCACTTGTGGAAGTTGGAAAAAAGTTGCCTTGGGGAAAAGATATCACGACAAAGAATATTGTAAAGAATGCTCCTTTTGTGATTCAATATATGGAATTGAAATCAATGTCAAAGGCCAGTGGTAAGGGGAAGGACTTGGAGAATGTGGAGATTGATTTGCAGTCTGCAGTGAGTGAGTGTTTAAGAAAAGAGCTTGAGTATGAGAGTGAGGTTTATAATTTAGAAGACGATTCTCAGTATTTTGAGTTACAACGCAGTAGATGGCAGGATGAATCGAATCGTGATAATGGGAACAGGCTCAATATTTCACATGAATGTTTATCCTTAATTTATAATAAATTTTTAGATGAAATGGCTGAGCTCAGGTCGTCGGGTGGTATTAATGAGGTCTATGGTACAAAGCAGGGAAGAAAAACTGGTTTTTACTCAAGTACAGAGTGGTGGGAGGGGAAATCAGAATTAAGCAAAAAGCTTCTATTGCAGCAATTATTGGAGACTGATATAGGGTCGCAGGGTATCCCACCATCATTTGTTTCGAGTTGCAATGCATACGAAAGGAGGAAGGAGAAAGTAAACGCTGCTAAGAAGCGAATCCAGAGGATTATGCACCCTTCAACTGCTCCAGCTGATGATGTAAATATTAAGAAGAAAAGAAAGCGTAAGGGAGCTGATGTTATAGAATGGGAAGATATAATAATTGAAACCCTTCTTCTCCATGGAGTACAGGAGGAGGAAATTGAGAAGGGCCATTACAAGGTCTTGTTGGACTTGTACGTCTTCACTTCTTCAACTTTTTAG

Protein sequence

MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGSVLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAYVSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNSPSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENVGMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKASGKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRLNISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLLQQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKRKGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF

Homology

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BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot
Match: O81787 (Plant-specific TFIIB-related protein PTF2 OS=Arabidopsis thaliana OX=3702 GN=PTF2 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-127
Identity = 246/538 (45.72%), Postives = 353/538 (65.61%), Query Frame = 0


Query: 5   CKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGSVLNY 64
           CK C+  +  RD+ +GN  C  CG ++E+DNY+AQLGGI GPQGT++RVGT G GSVL+Y
Sbjct: 3   CKRCNGSNFERDEDTGNSYCGGCGTLREYDNYEAQLGGIRGPQGTYIRVGTIGRGSVLDY 62

Query: 65  KDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAYVSMR 124
           KDKKIYEA  +IE+ T RL    +K+  ++ ++S +T+GE+G G+WFPIL+GAC Y  +R
Sbjct: 63  KDKKIYEANNLIEETTERLNL-GNKTEVIKSMISKLTDGEFGQGEWFPILIGACCYAVVR 122

Query: 125 KDSR-PLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNSPSF 184
           ++ +  LSM EVA  V CDLH+LG M+ RVV+ LDL   E   FD+VG   +   NSP  
Sbjct: 123 EEGKGVLSMEEVAYEVGCDLHQLGPMIKRVVDHLDL---ELREFDLVGLFTKTVTNSPRL 182

Query: 185 SRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENVGME 244
           + ++ D  E+I+KQG FL+ CA+KWFL+TGR+P+P+V AVL  V ++N V V I+++  +
Sbjct: 183 TDVDRDKKEKIIKQGTFLMNCALKWFLSTGRRPMPLVVAVLAFVVQVNGVKVKIDDLAKD 242

Query: 245 VHANVSTCKKRYRELLEALVEVGKK--LPWGKDITTKNIVKNAPFVIQYMELKSMSK-AS 304
              +++TCK RY+EL E LV+V ++  LPW KD+T KN++K++  +   ME KSM K   
Sbjct: 243 ASVSLTTCKTRYKELSEKLVKVAEEVGLPWAKDVTVKNVLKHSGTLFALMEAKSMKKRKQ 302

Query: 305 GKGKDLENVE-IDLQSAVSECLRKELEYESEVYNLEDD------SQYFELQRSRWQDESN 364
           G GK+L   +   ++  V +CL KE      +Y  +DD      S+YF+++  R     N
Sbjct: 303 GTGKELVRTDGFCVEDLVMDCLSKE-----SMYCYDDDARQDTMSRYFDVEGERQLSLCN 362

Query: 365 RDNGNRLNISHECLSLIYNKFLDEMA----ELRSSGGINEVYGTKQGRKTGFYSSTEWWE 424
            D+    NIS   LS  YN+F D +       RS G    ++  ++    G  S+  WW+
Sbjct: 363 YDD----NISENQLSTKYNEFEDRVCGGTLAKRSQGSSQSMW--QRRSVFGMVSTENWWK 422

Query: 425 GKSELSKKLLLQQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPA 484
           GKSELSK+LLL+ LLE D+G + +PPS++  C A ERR+EK+ AAK RI  I HPS   +
Sbjct: 423 GKSELSKRLLLKDLLEKDVGLEALPPSYIKGCVAVERRREKIKAAKLRINAIQHPSDNVS 482

Query: 485 D-----DVNIKKKRKRKGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 523
           +     ++   KK+++KG++ I+WED++I+TL+LH V EEEIEKGHYK LLDL+VF S
Sbjct: 483 EGALSLELEHSKKKRKKGSE-IDWEDLVIQTLVLHNVNEEEIEKGHYKTLLDLHVFNS 524

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BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot
Match: Q4R318 (Transcription factor IIIB 50 kDa subunit OS=Macaca fascicularis OX=9541 GN=BRF2 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.0e-07
Identity = 47/188 (25.00%), Postives = 92/188 (48.94%), Query Frame = 0


Query: 113 ILVGACAYVSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVG- 172
           +LVG C  ++ R+ + PL+M  + + +  DL     + M++V+ L L      + ++V  
Sbjct: 116 VLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSIYMQIVKLLGLDMPSLCLAELVKT 175

Query: 173 --SLERAARNSPSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLV-S 232
             S  +  + SPS      +  E+++ + + L++ A + +L TGR PLP++ A   L   
Sbjct: 176 YCSSFKLFQASPSVPAKYVEDKEKMLSRTLQLVELANETWLVTGRHPLPVITAATFLAWQ 235

Query: 233 KLNEVD-VSIENVGMEVHANVS---TCKKRYRELLEALVEVGKKLPWGK--DITTKNIVK 291
            L   D +S         ANV        R +ELL  L+ + ++L W +   +  +++VK
Sbjct: 236 SLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVVK 295

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BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot
Match: Q9HAW0 (Transcription factor IIIB 50 kDa subunit OS=Homo sapiens OX=9606 GN=BRF2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.3e-07
Identity = 47/190 (24.74%), Postives = 92/190 (48.42%), Query Frame = 0


Query: 113 ILVGACAYVSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVG- 172
           +LVG C  ++ R+ + PL+M  + + +  DL       M++V+ L L      + ++V  
Sbjct: 116 VLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLAELVKT 175

Query: 173 --SLERAARNSPSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLV-S 232
             S  +  + SPS      +  E+++ + + L++ A + +L TGR PLP++ A   L   
Sbjct: 176 YCSSFKLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVTGRHPLPVITAATFLAWQ 235

Query: 233 KLNEVD-VSIENVGMEVHANVS---TCKKRYRELLEALVEVGKKLPWGK--DITTKNIVK 292
            L   D +S         ANV        R +ELL  L+ + ++L W +   +  +++VK
Sbjct: 236 SLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRMAEQLAWLRVLRLDKRSVVK 295

[Close]

BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot
Match: A8KBY2 (Transcription factor IIIB 50 kDa subunit OS=Danio rerio OX=7955 GN=brf2 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.4e-07
Identity = 45/171 (26.32%), Postives = 84/171 (49.12%), Query Frame = 0


Query: 113 ILVGACAYVSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGS 172
           IL G C +   R++S P+ M  +   ++ D +++G +   + + L+L+ ++  +  ++ S
Sbjct: 114 ILAGCCMFHICRQNSWPVFMGTIGYLLDADNYQMGTIYQELTKSLNLQTTQVCITRMLES 173

Query: 173 LERAARNSP-SFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPM-VAAVLVLVSKL 232
                + +P     + +   +R+V Q   LL+ A   ++ TGR+P P+ +AAV V    L
Sbjct: 174 FCYDFKLAPDEVEEVFSVAQQRLVDQTSALLELAADTWILTGRRPFPLFLAAVYVAWQSL 233

Query: 233 N---EVDVSIENVGMEVHA-------NVSTCKKRYRELLEALVEVGKKLPW 272
           N    +  S+        A       +  T  KR  ELLE L ++G++LPW
Sbjct: 234 NPLARMKYSLMKFCKIAKAPEQLWCKSKDTINKRLNELLEVLCKLGRELPW 284

[Close]

BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot
Match: Q4V8D6 (Transcription factor IIIB 50 kDa subunit OS=Rattus norvegicus OX=10116 GN=Brf2 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.7e-07
Identity = 45/196 (22.96%), Postives = 92/196 (46.94%), Query Frame = 0


Query: 113 ILVGACAYVSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGS 172
           ++VG C  ++ R+ + PL+M  + + +  DL       M++V+ L L      + D+V S
Sbjct: 116 VVVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLLGLDVPSLCLADLVKS 175

Query: 173 L---ERAARNSPSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLV-S 232
                +  + SPS      +  ++++ + + L++ A + +L TGR PLP++ A   L   
Sbjct: 176 YCSSFKLFQASPSMPAKYVEDKDKMLSRTLLLVELANETWLVTGRHPLPIITAATFLAWQ 235

Query: 233 KLNEVD---------VSIENVGMEVHANVSTCKKRYRELLEALVEVGKKLPWGK--DITT 292
            L   D           + NV +   A       R +ELL  L+++  +L W +   +  
Sbjct: 236 SLRPSDRLTCSLARFCKLANVDLPYPA-----ASRLQELLAVLLQMASQLAWLQVLRLDK 295

Query: 293 KNIVKNAPFVIQYMEL 294
           +++VK+   ++Q+  +
Sbjct: 296 RSVVKHIGDLLQHRHM 306

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BLAST of Cucsat.G18523 vs. NCBI nr
Match: XP_004149065.1 (plant-specific TFIIB-related protein PTF2 [Cucumis sativus] >KGN65641.1 hypothetical protein Csa_019679 [Cucumis sativus])

HSP 1 Score: 1031 bits (2667), Expect = 0.0
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
           GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS
Sbjct: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL
Sbjct: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525
           KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525

[Close]

BLAST of Cucsat.G18523 vs. NCBI nr
Match: XP_008443157.1 (PREDICTED: transcription factor IIIB 50 kDa subunit [Cucumis melo] >KAA0057033.1 transcription factor IIIB 50 kDa subunit [Cucumis melo var. makuwa])

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 500/525 (95.24%), Postives = 511/525 (97.33%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRSIFRDDVSGN ICSSCGIVQEF+NY+AQLGGINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK+YEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPLSMSEVASAVECDLHELGRMVMRVV+FLDLRGSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVS+ENV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENV IDLQSAVSECLRKELEYESEV NLEDDSQYFELQ SRW DESN DNGNRL
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYNKFLDEMAELRSSGGIN+VYG KQGRK GFYSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAY+RRKEKVNAAKKRIQRIMHPS APA DVNIKKKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525
           KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDL+VFTSSTF
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTSSTF 525

[Close]

BLAST of Cucsat.G18523 vs. NCBI nr
Match: XP_038893651.1 (plant-specific TFIIB-related protein PTF2 [Benincasa hispida])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 470/520 (90.38%), Postives = 493/520 (94.81%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRS+FRDDVSGN ICSSCGIVQEFDNY+AQL GINGP GTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSLFRDDVSGNLICSSCGIVQEFDNYEAQLSGINGPLGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKI+EAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIFEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVASAVECDLHELGRMV+RVV+FLDL+G EFPVFDIVGSLERA RNS
Sbjct: 121 VSMRKDNRPLPMSEVASAVECDLHELGRMVIRVVDFLDLKGPEFPVFDIVGSLERATRNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSR++ADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSI+NV
Sbjct: 181 PSFSRVDADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIDNV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELLEALV+VGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVKVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENV +DLQSAVSECLRKELEYESEV NLEDDSQYFELQ S W+DES+ DNGNRL
Sbjct: 301 GKGKDLENVGVDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSSWRDESSCDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY+KFLDEMA L+SSGGINEVYG KQGRK GFYSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYHKFLDEMAHLKSSGGINEVYGRKQGRKAGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLE DIGSQGIPPSFVSSCNAY+RRKEK+ AAKKRIQRIM PS AP  DVNI+KKRKR
Sbjct: 421 QQLLEIDIGSQGIPPSFVSSCNAYKRRKEKIIAAKKRIQRIMLPSNAPIGDVNIEKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVF 520
           KG + IEWEDIIIETLLLHGV+EEEIEKGHY  LLDL+VF
Sbjct: 481 KGTNGIEWEDIIIETLLLHGVKEEEIEKGHYNALLDLHVF 520

[Close]

BLAST of Cucsat.G18523 vs. NCBI nr
Match: KAG6573303.1 (Plant-specific TFIIB-related protein PTF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 857 bits (2213), Expect = 2.68e-310
Identity = 435/522 (83.33%), Postives = 472/522 (90.42%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RS+FRDDVSGN ICSSCGIVQEFDNYDAQLGG+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVAS +ECDLHELGRMV+RVV+FLDL+G  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFS++EAD L+ IVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVS LNEV+VSI++V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMS   
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           G+GK+ E+V IDLQ+AVSECLRKEL+YESEV NLEDDSQYFEL  + W+DES+ D+ N+L
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMA LRSSGGINE+YG K GRK GF SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
            QLLETDIG  G+PPSFV+SCNAY+RRKEK+NAAK RIQRIM PS A   DVN +K+RKR
Sbjct: 421 HQLLETDIGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522
           K A  IEWEDIIIETLLLH V+EEEIEKGHY  LLDL+VF+S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

[Close]

BLAST of Cucsat.G18523 vs. NCBI nr
Match: XP_023542889.1 (plant-specific TFIIB-related protein PTF2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856 bits (2212), Expect = 3.81e-310
Identity = 434/522 (83.14%), Postives = 471/522 (90.23%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RS+FRDDVSGN ICSSCGIVQEFDNYDAQLGG+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVAS +ECDLHELGRMV+RVV+FLDL+G  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFS++EAD L+ IVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVS LNEV+VSI++V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMS   
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           G+GK+ E+V IDLQ+AVSECLRKEL+YESEV NLEDDSQYFEL  + W+DES+ D+ N+L
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMA LRSSGGINE+YG K GRK GF SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
            QLLETD+G  G+PPSFV+SCNAY+RRKEK+NAAK RIQRIM PS A   DVN +K+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNAAIGDVNAEKERKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522
           K A  IEWEDIIIETLLLH V+EEEIEKGHY  LLDL+VF S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFGS 522

[Close]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL
Match: A0A0A0M0A0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G477510 PE=4 SV=1)

HSP 1 Score: 1031 bits (2667), Expect = 0.0
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
           GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS
Sbjct: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL
Sbjct: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525
           KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525

[Close]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL
Match: A0A5A7UMA0 (Transcription factor IIIB 50 kDa subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002190 PE=4 SV=1)

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 500/525 (95.24%), Postives = 511/525 (97.33%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRSIFRDDVSGN ICSSCGIVQEF+NY+AQLGGINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK+YEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPLSMSEVASAVECDLHELGRMVMRVV+FLDLRGSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVS+ENV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENV IDLQSAVSECLRKELEYESEV NLEDDSQYFELQ SRW DESN DNGNRL
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYNKFLDEMAELRSSGGIN+VYG KQGRK GFYSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAY+RRKEKVNAAKKRIQRIMHPS APA DVNIKKKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525
           KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDL+VFTSSTF
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTSSTF 525

[Close]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL
Match: A0A1S3B6X4 (transcription factor IIIB 50 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103486826 PE=4 SV=1)

HSP 1 Score: 979 bits (2532), Expect = 0.0
Identity = 500/525 (95.24%), Postives = 511/525 (97.33%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCK+C+SRSIFRDDVSGN ICSSCGIVQEF+NY+AQLGGINGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKDCNSRSIFRDDVSGNLICSSCGIVQEFENYEAQLGGINGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKK+YEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY
Sbjct: 61  VLNYKDKKVYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPLSMSEVASAVECDLHELGRMVMRVV+FLDLRGSEFPVFDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLSMSEVASAVECDLHELGRMVMRVVDFLDLRGSEFPVFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVS+ENV
Sbjct: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSMENV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMS AS
Sbjct: 241 AMEVHAKVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSNAS 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           GKGKDLENV IDLQSAVSECLRKELEYESEV NLEDDSQYFELQ SRW DESN DNGNRL
Sbjct: 301 GKGKDLENVGIDLQSAVSECLRKELEYESEVNNLEDDSQYFELQGSRWCDESNCDNGNRL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIYNKFLDEMAELRSSGGIN+VYG KQGRK GFYSSTEWWEGKS LSKKLLL
Sbjct: 361 NISHECLSLIYNKFLDEMAELRSSGGINDVYGRKQGRKAGFYSSTEWWEGKSVLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
           QQLLETDIGSQGIPPSFVSSCNAY+RRKEKVNAAKKRIQRIMHPS APA DVNIKKKRKR
Sbjct: 421 QQLLETDIGSQGIPPSFVSSCNAYKRRKEKVNAAKKRIQRIMHPSNAPAGDVNIKKKRKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTSSTF 525
           KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDL+VFTSSTF
Sbjct: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLHVFTSSTF 525

[Close]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL
Match: A0A6J1JZD6 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita maxima OX=3661 GN=LOC111490217 PE=4 SV=1)

HSP 1 Score: 853 bits (2203), Expect = 4.32e-309
Identity = 433/522 (82.95%), Postives = 470/522 (90.04%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RS+FRDDVSGN ICSSCGIVQEFDNYDAQLGG+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQK+IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQKIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVAS +ECDLHELGRMV+RVV+FLDL+G  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFS++EAD L+ IVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVS LNEV+VSI++V
Sbjct: 181 PSFSKVEADTLDIIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMS   
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           G+GK+ E+V IDLQ+AVSECLRKEL+YESEV NLEDDSQYFEL  + W DES+ D+ N+L
Sbjct: 301 GQGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWHDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMA LRSSG INE+YG K GRK GF SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGEINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
            QLLETD+G  G+PPSFV+SCNAY+RRKEK+NAAK RIQRIM PS A   DVN +K+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSHAAIGDVNAEKERKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522
           K A  IEWEDIIIETLLLH V+EEEIEKGHY  LLDL+VF+S
Sbjct: 481 KRAVGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

[Close]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL
Match: A0A6J1GSR8 (plant-specific TFIIB-related protein PTF2 OS=Cucurbita moschata OX=3662 GN=LOC111457096 PE=4 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 8.72e-309
Identity = 431/522 (82.57%), Postives = 470/522 (90.04%), Query Frame = 0


Query: 1   MSGSCKNCHSRSIFRDDVSGNQICSSCGIVQEFDNYDAQLGGINGPQGTFVRVGTSGSGS 60
           MSGSCKNC  RS+FRDDVSGN ICSSCGIVQEFDNYDAQLGG+NGPQGTFVRVGTSGSGS
Sbjct: 1   MSGSCKNCSGRSLFRDDVSGNLICSSCGIVQEFDNYDAQLGGLNGPQGTFVRVGTSGSGS 60

Query: 61  VLNYKDKKIYEAQKVIEDITFRLGFSASKSNDVRILVSTITEGEYGLGDWFPILVGACAY 120
           VLNYKDKKIYEAQ +IEDITFRLGFSASKSNDVRILVSTITEGEYG GDWF ILVGAC+Y
Sbjct: 61  VLNYKDKKIYEAQNIIEDITFRLGFSASKSNDVRILVSTITEGEYGQGDWFSILVGACSY 120

Query: 121 VSMRKDSRPLSMSEVASAVECDLHELGRMVMRVVEFLDLRGSEFPVFDIVGSLERAARNS 180
           VSMRKD+RPL MSEVAS +ECDLHELGRMV+RVV+FLDL+G  FP FDIVGSLERAARNS
Sbjct: 121 VSMRKDNRPLPMSEVASTIECDLHELGRMVLRVVDFLDLKGPAFPGFDIVGSLERAARNS 180

Query: 181 PSFSRLEADILERIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSKLNEVDVSIENV 240
           PSFS++EAD L+ IVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVS LNEV+VSI++V
Sbjct: 181 PSFSKVEADTLDVIVKQGIFLLQCAMKWFLTTGRQPLPMVAAVLVLVSGLNEVNVSIDDV 240

Query: 241 GMEVHANVSTCKKRYRELLEALVEVGKKLPWGKDITTKNIVKNAPFVIQYMELKSMSKAS 300
            MEVHA VSTCKKRYRELL ALVEVGKKLPWGKDIT +NIVKNAPFVIQYMELKSMS   
Sbjct: 241 AMEVHAKVSTCKKRYRELLGALVEVGKKLPWGKDITKRNIVKNAPFVIQYMELKSMSNTC 300

Query: 301 GKGKDLENVEIDLQSAVSECLRKELEYESEVYNLEDDSQYFELQRSRWQDESNRDNGNRL 360
           G+GK+ E+V IDLQ+AVSECLRKEL+YESEV NLEDDSQYFEL  + W+DES+ D+ N+L
Sbjct: 301 GRGKNFEDVGIDLQAAVSECLRKELQYESEVNNLEDDSQYFELIGNSWRDESSCDDRNKL 360

Query: 361 NISHECLSLIYNKFLDEMAELRSSGGINEVYGTKQGRKTGFYSSTEWWEGKSELSKKLLL 420
           NISHECLSLIY KFLDEMA LRSSGGINE+YG K GRK GF SSTEWWEGKS+LSKKLLL
Sbjct: 361 NISHECLSLIYTKFLDEMAHLRSSGGINEIYGRKLGRKAGFQSSTEWWEGKSQLSKKLLL 420

Query: 421 QQLLETDIGSQGIPPSFVSSCNAYERRKEKVNAAKKRIQRIMHPSTAPADDVNIKKKRKR 480
            QLLETD+G  G+PPSFV+SCNAY+RRKEK+NAAK RIQRIM PS     +VN +K+RKR
Sbjct: 421 HQLLETDVGLNGMPPSFVTSCNAYKRRKEKINAAKNRIQRIMLPSNTAIGNVNAEKERKR 480

Query: 481 KGADVIEWEDIIIETLLLHGVQEEEIEKGHYKVLLDLYVFTS 522
           K A  IEWEDIIIETLLLH V+EEEIEKGHY  LLDL+VF+S
Sbjct: 481 KRAIGIEWEDIIIETLLLHDVKEEEIEKGHYNALLDLHVFSS 522

The following BLAST results are available for this feature:

BLAST of Cucsat.G18523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81787	1.1e-127	45.72	Plant-specific TFIIB-related protein PTF2 OS=Arabidopsis thaliana OX=3702 GN=PTF...	[more]
Q4R318	2.0e-07	25.00	Transcription factor IIIB 50 kDa subunit OS=Macaca fascicularis OX=9541 GN=BRF2 ...	[more]
Q9HAW0	3.3e-07	24.74	Transcription factor IIIB 50 kDa subunit OS=Homo sapiens OX=9606 GN=BRF2 PE=1 SV...	[more]
A8KBY2	4.4e-07	26.32	Transcription factor IIIB 50 kDa subunit OS=Danio rerio OX=7955 GN=brf2 PE=2 SV=...	[more]
Q4V8D6	5.7e-07	22.96	Transcription factor IIIB 50 kDa subunit OS=Rattus norvegicus OX=10116 GN=Brf2 P...	[more]

BLAST of Cucsat.G18523 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149065.1	0.0	100.00	plant-specific TFIIB-related protein PTF2 [Cucumis sativus] >KGN65641.1 hypothet...	[more]
XP_008443157.1	0.0	95.24	PREDICTED: transcription factor IIIB 50 kDa subunit [Cucumis melo] >KAA0057033.1...	[more]
XP_038893651.1	0.0	90.38	plant-specific TFIIB-related protein PTF2 [Benincasa hispida]	[more]
KAG6573303.1	2.68e-310	83.33	Plant-specific TFIIB-related protein PTF2, partial [Cucurbita argyrosperma subsp...	[more]
XP_023542889.1	3.81e-310	83.14	plant-specific TFIIB-related protein PTF2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G18523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0A0	0.0	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G477510 PE=4 SV=1	[more]
A0A5A7UMA0	0.0	95.24	Transcription factor IIIB 50 kDa subunit OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A1S3B6X4	0.0	95.24	transcription factor IIIB 50 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103486826...	[more]
A0A6J1JZD6	4.32e-309	82.95	plant-specific TFIIB-related protein PTF2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GSR8	8.72e-309	82.57	plant-specific TFIIB-related protein PTF2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 441..461
None	No IPR available	GENE3D	1.10.472.10		coord: 62..162 e-value: 2.5E-8 score: 35.8
None	No IPR available	GENE3D	2.20.25.10		coord: 2..39 e-value: 1.1E-5 score: 26.6
None	No IPR available	GENE3D	1.10.472.10		coord: 183..265 e-value: 1.9E-7 score: 33.2
None	No IPR available	PANTHER	PTHR11618:SF13	TRANSCRIPTION INITIATION FACTOR IIB	coord: 3..298
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 4..37
IPR000812	Transcription factor TFIIB	PRINTS	PR00685	TIFACTORIIB	coord: 142..157 score: 25.83 coord: 214..230 score: 32.55 coord: 246..260 score: 29.33 coord: 17..37 score: 33.65
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 3..298
IPR036915	Cyclin-like superfamily	SUPERFAMILY	47954	Cyclin-like	coord: 193..262
IPR036915	Cyclin-like superfamily	SUPERFAMILY	47954	Cyclin-like	coord: 72..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G18523.T1	Cucsat.G18523.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070897	transcription preinitiation complex assembly
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0097550	transcription preinitiation complex
molecular_function	GO:0017025	TBP-class protein binding