Cucsat.G1849 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1849
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Exonuclease domain-containing protein
Location	ctg1002: 3261290 .. 3263077 (-)
RNA-Seq Expression	Cucsat.G1849
Synteny	Cucsat.G1849

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAGAAGCTAAGGACTTCTTCATCCACGGACCAAGCTCTTGACTTTAATTTCAAGACAACCAATCAAACCCTCAACACCAAGAATAATCCTCTCTTTTCTTCGCCCATTTATAACCTCTTCAATCTCCCATGTCATTGAACATTAAGAAAACAAAACTTTATCTTGAAACTATTTGACATAAATGCCAATGGATATGGAGGCTTCAAGTTCATTCAAAGAAGGTGCAGCTGAGATTGTATTTTTCGACTTGGAAACTACCGTACCCAAAAGAGTTGGGCAACGGTTTCGAGTATTGGAATTTGGTGCTATTGTTGTCTGCCCAATGAAACTTGTAGAGCTCGAGAGCTTTACTACTCTCATTAGGCCAACGGATTTATCTGCTGTTGCGTTGAGGTCTAGCCGACCTGACGGAATAACTAGAGCAGCTGTTGCAACTGCACCATTGTTTGAGGAAGTGGCTGACAAGATATTCAGCATTTTGAATGGAAGGATATGGGCAGGTCACAACATTCGAAGATTCGACTGTGTTCGTATTAAGGAGGCATTTGCAGAGATTGGCAGGCCTGCTCCTATCCCAGTTGGGATGGTTGATTCTTTAGGAGTCTTAACGAACAAATTTGGCAAGAGAGCCGGGAACATGAAGGTGAGAAATAACGAACTTTTGAACCTTGAAAACCATAACATATAGCATACAACGTTTGGCAAAATAGTTCTCATATATTTTGAACTGTTTTATATTTAGATGGCATCATTGGCTTCTTACTTTCAGTTGGGACAGCAAAAGCACAGGTATCAATACAATTGATCGACTCCCTTCTTTGTATGTGTTAACTTGTATCATCTTAGTTCACAAACTGACAAAAGTATTTTTGTGCAGGAGCCTCGACGATGTTCGTATGAACTTGGAGGTTCTCAAACATTGTGCTACTGTTCTATTTCTGGTATGTATACAAAAATTTCTACCACATATGCCGCTCTTCTTGGACAAATGAGTTATCTAAAACAATTTCTCCACCAGGAATCGACCCTCCCAAGCATATTGCAAGAGAAGTGGAAGACTTCATCCACCAAAACAACTCGAAGCAGAGCCAATGGGAAGCTGAATTGCAGAGAGGAAAGTAGTCGAAAATCTCCTCCATCATCACCGGGATATCAACTGAGATCAGTTCCTTATTCAAGGGAAGGATTAGGCATTGGAAAGGTTTAGTCATAGGTCTTTCTTTATATTTCAATGAAATGTTTTCCCCAACCAGTTTATGAAAATATGCTGAATTGAAACAGATGACAGCTAAGGTAAAGAATTTATTCTACATGGCTCAGGGAAAACAGCATCTCAACGTCTTACTTAAACATTCTCGACTGCTATTACGGTGACTTGAAACCACATGGTTGTCCTTTGCACTCCAAATTGTCATTGTTTTGTAATAAGCTGGAAAGGAAGACAACAATATATTTTCTATGAAGGACAGGAAGTGTGGACGTAAAAGAGCGGTATGTCATCTTCTTGATGAAAGTACCTTGTGCTTTTGGAGAGTGATTCTTGTTTGTTCTTGGGAGGGATTCTTTCATGTACATATATGAAAATATATAACCAGAAATCCATTGGTTTATTCTTTCATTACTTCATCCTGTTTTGTTTTTAAGTTATGAGTACATCTTCAAATTTTATGCAAAATGAACCAAGTCCATTTCCTATACCCTTTTCCCTTTCAAATGGAACCTTTCTTTCAAAACAACACATGGCTGAAATCATCTCTCACCAACCATCTGGATTTTGAACAGCACAGTTAAATATATACAAATACACACATAAAGCTTTAAATCTCAGTTTCATGGCTCTGCCATGAATTCTTTAAATCAATTTGTTACCAAGAGAAAATTTAAACTTGGAACAGACCACATTTATAATTTTGTCCATTAAACTTGTTCGATAAAAATAAAAAATCAACTTTTAAATCTAACTGAAATTAGGTTAATTAATTAACATAATCTGGAGGTGGAAGATTTGATCATTCATCCTACAATTAATTAGTAAATATATGAAAAAAAATTTGTAACTCTCCTAGTCAATTTGTAAGTATTTATCCCTCCCACAAGTTTTCTTTCTTCAATTCTTTCTTAAGTTTTTATTCTAAACTTCAAGTGGTGGAGTGGCAACCCTACTACACCTTTTAGATTTAATTATAATGTTTTCAACTAACTATAACCTATAAATTAACTATAGTATAATAATTTAAAAATCATCTTCCTCAAAAATACTTTGTGACGAGACACAAATTACTATAATCTACGAGCTATTATAATTGACAAACCATAATAATCACTAACCAACTTAGCACTTCAAACAATCTCCTCTATTTAAAAATTAACAAAATATTTTATTGGATTTGACATAGTTAGGTATGGTTGATAAATTGTGATGTTATGTTATATTTATAAATAAGATTAGAAAATGTTGTAATTTTGAGGGAAACAAATAAACGTCAAATCAGGAAGCTACAATTGGATTCCAACTAAAGTTTGATAGCAAAAATCCAAAAGGGATGGTGTATGTTTCAGAGCCCCACTTTTGAATTAATTCACATCCTCATTATTCATACATATATTGTAATAATTGAACACACCCTTAAAAAACTATAACGTGTAAGTACTCCTTTTGTAATGTTTTATTTATTTATCTCATAAGTAATCTACCATACCCTTACCCCCTCAAATATCACAATGACATGGACGACAAGGGGCCTCCAAACAAGGGGCCTTCAATGGCTTATAAACAGCAATTTTTTTAATTTTTTAGCTAAAAAAGTAGATCAAATTTCACGAGATCTCTTCTTTATCACATTCACAACTACCAGACTCTTCGGAAAAGTTGGATAAAAAGGAAAAGCAAACAGAGCTTTTGGTAAAGAAAAAGGGCCACACCAATTTTTAACCCCAAACAAAATAGTAGTGGGGATGTATATGAAGTTTCAGAAATGCATTGGTTTGGGATCAGTTGTTGGAATTTACAAAGAATTGAATTGACAAGTTTCCAACCAACTCTGTTCTATATTGTGATACAATTCTACTGTTTCATGCCTCTGTTTCAGCTTTTGCACAATTTCCTGTTTATAGTTATACAAACCCATGTGACACCTCTAGTTTTCTTTCGTTTCATTTTGGACTTTATGGCACCCACCCACCTTCCATTCATTTACTTATTTACTTTATTCTTCTTTAACCGATTAAAACTACCGTTTTATTGAATTGAGAAAAATAAACAGAAATCATACATAATATATTATCACGTGAATAAATGGGGTGGGTTTCCTGCACATTGATGGGGATGAGGATGGGACCTCCATCGCCACCTATTGGATACAAGGGAACATTCTAGATTACCCTTTACAATTTCATATCTAATTTCATCGAATTATTAAACAAAAAAATTGAAAGTTCTTTTGTTAGAAGGTAAATAATGTCTTTTCAATTAAACTATTCTGGATTATAAGCTAAGAAAAACAATGGATTGATTGATTGATTGATATATATGGATGTATGCATTAATTAAGAACAATGAAGAAGGGGGAATGGTATTGCTGG

Coding sequence (CDS)

ATGCCAATGGATATGGAGGCTTCAAGTTCATTCAAAGAAGGTGCAGCTGAGATTGTATTTTTCGACTTGGAAACTACCGTACCCAAAAGAGTTGGGCAACGGTTTCGAGTATTGGAATTTGGTGCTATTGTTGTCTGCCCAATGAAACTTGTAGAGCTCGAGAGCTTTACTACTCTCATTAGGCCAACGGATTTATCTGCTGTTGCGTTGAGGTCTAGCCGACCTGACGGAATAACTAGAGCAGCTGTTGCAACTGCACCATTGTTTGAGGAAGTGGCTGACAAGATATTCAGCATTTTGAATGGAAGGATATGGGCAGGTCACAACATTCGAAGATTCGACTGTGTTCGTATTAAGGAGGCATTTGCAGAGATTGGCAGGCCTGCTCCTATCCCAGTTGGGATGGTTGATTCTTTAGGAGTCTTAACGAACAAATTTGGCAAGAGAGCCGGGAACATGAAGATGGCATCATTGGCTTCTTACTTTCAGTTGGGACAGCAAAAGCACAGGAGCCTCGACGATGTTCGTATGAACTTGGAGGTTCTCAAACATTGTGCTACTGTTCTATTTCTGGAATCGACCCTCCCAAGCATATTGCAAGAGAAGTGGAAGACTTCATCCACCAAAACAACTCGAAGCAGAGCCAATGGGAAGCTGAATTGCAGAGAGGAAAGTAGTCGAAAATCTCCTCCATCATCACCGGGATATCAACTGAGATCAGTTCCTTATTCAAGGGAAGGATTAGGCATTGGAAAGGTTTAG

Protein sequence

MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRSVPYSREGLGIGKV

Homology

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BLAST of Cucsat.G1849 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.0e-85
Identity = 167/253 (66.01%), Postives = 195/253 (77.08%), Query Frame = 0


Query: 17  EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRSSRPD 76
           EIVFFDLET VP + GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V++RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 77  GITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIPVGMV 136
           GITRA V  AP FE+VA+KI  +LNGRIWAGHNIRRFDCVRIKEAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 137 DSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196
           DSLG+L++KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 197 SILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRSVPYSREGLGIGKGKQH 256
           + L+ KW TSS   TRSR N ++      ++++ P S G   +     +  L   +G Q 
Sbjct: 189 NHLEGKWHTSSKIMTRSRRNYQI------AQRAMPYSKGSLEKMTQNVKNLLSKAQGNQT 248

Query: 257 LNVLLKHSRLLLR 270
           L  L+ HS  LLR
Sbjct: 249 LQSLINHSHSLLR 255

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BLAST of Cucsat.G1849 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.3e-68
Identity = 139/233 (59.66%), Postives = 172/233 (73.82%), Query Frame = 0


Query: 7   ASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLS 66
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIG 126
            ++  + R  GITR  V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRI+EAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 187 TVLFLESTLPSILQEKWKTSSTKTTRSRANGKL---NCREE--SSRKSPPSSP 235
           TVLFLES++P IL +    S  K+ R+R+NGKL     RE   SS  SP + P
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 234

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BLAST of Cucsat.G1849 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-66
Identity = 129/202 (63.86%), Postives = 157/202 (77.72%), Query Frame = 0


Query: 13  EGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRS 72
           E  +EI FFD+ETTVPKR GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++  S
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 73  SRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIP 132
            R +GI R  V  APLF ++AD ++ IL+GRIWAGHNI RFDC RI+EAFAEIGR  P P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 133 VGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLE 192
            G +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 193 STLPSILQEKWKTSSTKTTRSR 215
           S+LP    +   +  T ++R R
Sbjct: 184 SSLPYAHVDNSVSPETISSRRR 204

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BLAST of Cucsat.G1849 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-64
Identity = 127/222 (57.21%), Postives = 165/222 (74.32%), Query Frame = 0


Query: 13  EGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRS 72
           E  +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +  RS
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 73  SRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIP 132
            + +GI R  V +A  F ++AD ++ IL+GRIWAGHNI +FDC RI+EAFAEIGR  P P
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 133 VGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLE 192
            G +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 193 STLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSP 235
           S+LP  L E   T++T  T SR       R  + +KSP  SP
Sbjct: 191 SSLPDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

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BLAST of Cucsat.G1849 vs. NCBI nr
Match: XP_004143479.1 (protein NEN4 isoform X2 [Cucumis sativus])

HSP 1 Score: 522 bits (1345), Expect = 1.22e-186
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI
Sbjct: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGKGKQHLNVLLKHSRLLLR 269
           VPYSREGLGIGKGKQHLNVLLKHSRLLLR
Sbjct: 241 VPYSREGLGIGKGKQHLNVLLKHSRLLLR 269

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BLAST of Cucsat.G1849 vs. NCBI nr
Match: XP_011657983.1 (protein NEN4 isoform X1 [Cucumis sativus] >KAE8647561.1 hypothetical protein Csa_003314 [Cucumis sativus])

HSP 1 Score: 513 bits (1321), Expect = 8.96e-183
Identity = 269/282 (95.39%), Postives = 269/282 (95.39%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI
Sbjct: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSREGLGIGK             GKQHLNVLLKHSRLLLR
Sbjct: 241 VPYSREGLGIGKMTAKVKNLFYMAQGKQHLNVLLKHSRLLLR 282

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BLAST of Cucsat.G1849 vs. NCBI nr
Match: XP_008440629.1 (PREDICTED: protein NEN4 isoform X3 [Cucumis melo] >KAA0036283.1 protein NEN4 isoform X3 [Cucumis melo var. makuwa] >TYK12677.1 protein NEN4 isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 490 bits (1261), Expect = 1.25e-173
Identity = 256/282 (90.78%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 VPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

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BLAST of Cucsat.G1849 vs. NCBI nr
Match: XP_016899301.1 (PREDICTED: protein NEN4 isoform X1 [Cucumis melo])

HSP 1 Score: 484 bits (1246), Expect = 2.79e-171
Identity = 256/286 (89.51%), Postives = 261/286 (91.26%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFL----ESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGY 240
           VLKHCATVLFL    ESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGY
Sbjct: 181 VLKHCATVLFLFLHQESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGY 240

Query: 241 QLRSVPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           QLRSVPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 QLRSVPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 286

[Close]

BLAST of Cucsat.G1849 vs. NCBI nr
Match: XP_016899303.1 (PREDICTED: protein NEN4 isoform X4 [Cucumis melo])

HSP 1 Score: 483 bits (1244), Expect = 4.69e-171
Identity = 255/282 (90.43%), Postives = 260/282 (92.20%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKH SLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKH-SLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 VPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 281

[Close]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL
Match: A0A0A0KIX8 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G500610 PE=4 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 5.91e-187
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI
Sbjct: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGKGKQHLNVLLKHSRLLLR 269
           VPYSREGLGIGKGKQHLNVLLKHSRLLLR
Sbjct: 241 VPYSREGLGIGKGKQHLNVLLKHSRLLLR 269

[Close]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL
Match: A0A5D3CL58 (Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002650 PE=4 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 6.05e-174
Identity = 256/282 (90.78%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 VPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

[Close]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL
Match: A0A1S3B2A8 (protein NEN4 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 6.05e-174
Identity = 256/282 (90.78%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 VPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

[Close]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL
Match: A0A1S4DTI3 (protein NEN4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 1.35e-171
Identity = 256/286 (89.51%), Postives = 261/286 (91.26%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180

Query: 181 VLKHCATVLFL----ESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGY 240
           VLKHCATVLFL    ESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGY
Sbjct: 181 VLKHCATVLFLFLHQESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGY 240

Query: 241 QLRSVPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           QLRSVPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 QLRSVPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 286

[Close]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL
Match: A0A1S4DTM0 (protein NEN4 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 2.27e-171
Identity = 255/282 (90.43%), Postives = 260/282 (92.20%), Query Frame = 0


Query: 1   MPMDMEASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLI 60
           MPMDMEAS SFKEGA EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESF TLI
Sbjct: 1   MPMDMEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLI 60

Query: 61  RPTDLSAVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKE 120
           +PTDLSAVALRSSRPDGITR AVATAPLFE+VADKIFSILNGRIWAGHNIRRFDCVRIKE
Sbjct: 61  KPTDLSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKE 120

Query: 121 AFAEIGRPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLE 180
           AFAEIGRPAP PVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKH SLDDVRMNLE
Sbjct: 121 AFAEIGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKH-SLDDVRMNLE 180

Query: 181 VLKHCATVLFLESTLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRS 240
           VLKHCATVLFLESTLPSILQ+KWKTS TKTTR+RANGKL+CREESSRKSPPSSPGYQLRS
Sbjct: 181 VLKHCATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRS 240

Query: 241 VPYSREGLGIGK-------------GKQHLNVLLKHSRLLLR 269
           VPYSRE LGIGK             GKQHLNVLLKHSR LLR
Sbjct: 241 VPYSRERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 281

[Close]

BLAST of Cucsat.G1849 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.5e-86
Identity = 167/253 (66.01%), Postives = 195/253 (77.08%), Query Frame = 0


Query: 17  EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRSSRPD 76
           EIVFFDLET VP + GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V++RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 77  GITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIPVGMV 136
           GITRA V  AP FE+VA+KI  +LNGRIWAGHNIRRFDCVRIKEAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 137 DSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196
           DSLG+L++KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 197 SILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSPGYQLRSVPYSREGLGIGKGKQH 256
           + L+ KW TSS   TRSR N ++      ++++ P S G   +     +  L   +G Q 
Sbjct: 189 NHLEGKWHTSSKIMTRSRRNYQI------AQRAMPYSKGSLEKMTQNVKNLLSKAQGNQT 248

Query: 257 LNVLLKHSRLLLR 270
           L  L+ HS  LLR
Sbjct: 249 LQSLINHSHSLLR 255

[Close]

BLAST of Cucsat.G1849 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-69
Identity = 139/233 (59.66%), Postives = 172/233 (73.82%), Query Frame = 0


Query: 7   ASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLS 66
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIG 126
            ++  + R  GITR  V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRI+EAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 187 TVLFLESTLPSILQEKWKTSSTKTTRSRANGKL---NCREE--SSRKSPPSSP 235
           TVLFLES++P IL +    S  K+ R+R+NGKL     RE   SS  SP + P
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 234

[Close]

BLAST of Cucsat.G1849 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 8.9e-68
Identity = 129/202 (63.86%), Postives = 157/202 (77.72%), Query Frame = 0


Query: 13  EGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRS 72
           E  +EI FFD+ETTVPKR GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++  S
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 73  SRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIP 132
            R +GI R  V  APLF ++AD ++ IL+GRIWAGHNI RFDC RI+EAFAEIGR  P P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 133 VGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLE 192
            G +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 193 STLPSILQEKWKTSSTKTTRSR 215
           S+LP    +   +  T ++R R
Sbjct: 184 SSLPYAHVDNSVSPETISSRRR 204

[Close]

BLAST of Cucsat.G1849 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 8.3e-66
Identity = 127/222 (57.21%), Postives = 165/222 (74.32%), Query Frame = 0


Query: 13  EGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLSAVALRS 72
           E  +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +  RS
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 73  SRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPIP 132
            + +GI R  V +A  F ++AD ++ IL+GRIWAGHNI +FDC RI+EAFAEIGR  P P
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 133 VGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLE 192
            G +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 193 STLPSILQEKWKTSSTKTTRSRANGKLNCREESSRKSPPSSP 235
           S+LP  L E   T++T  T SR       R  + +KSP  SP
Sbjct: 191 SSLPDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of Cucsat.G1849 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.6e-61
Identity = 130/233 (55.79%), Postives = 161/233 (69.10%), Query Frame = 0


Query: 7   ASSSFKEGAAEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFTTLIRPTDLS 66
           ASS   +  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVALRSSRPDGITRAAVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIG 126
            ++  + R  GITR  V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRI+EAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPIPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLE      
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE------ 181

Query: 187 TVLFLESTLPSILQEKWKTSSTKTTRSRANGKL---NCREE--SSRKSPPSSP 235
                ES++P IL +    S  K+ R+R+NGKL     RE   SS  SP + P
Sbjct: 182 -----ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDP 223

The following BLAST results are available for this feature:

BLAST of Cucsat.G1849 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJ23	2.0e-85	66.01	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]
Q9CA74	2.3e-68	59.66	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q9FLR0	1.2e-66	63.86	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	1.2e-64	57.21	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]

BLAST of Cucsat.G1849 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143479.1	1.22e-186	100.00	protein NEN4 isoform X2 [Cucumis sativus]	[more]
XP_011657983.1	8.96e-183	95.39	protein NEN4 isoform X1 [Cucumis sativus] >KAE8647561.1 hypothetical protein Csa...	[more]
XP_008440629.1	1.25e-173	90.78	PREDICTED: protein NEN4 isoform X3 [Cucumis melo] >KAA0036283.1 protein NEN4 iso...	[more]
XP_016899301.1	2.79e-171	89.51	PREDICTED: protein NEN4 isoform X1 [Cucumis melo]	[more]
XP_016899303.1	4.69e-171	90.43	PREDICTED: protein NEN4 isoform X4 [Cucumis melo]	[more]

BLAST of Cucsat.G1849 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIX8	5.91e-187	100.00	Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G500610...	[more]
A0A5D3CL58	6.05e-174	90.78	Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3B2A8	6.05e-174	90.78	protein NEN4 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1	[more]
A0A1S4DTI3	1.35e-171	89.51	protein NEN4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1	[more]
A0A1S4DTM0	2.27e-171	90.43	protein NEN4 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1	[more]

BLAST of Cucsat.G1849 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39810.1	1.5e-86	66.01	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	1.6e-69	59.66	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G07710.1	8.9e-68	63.86	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	8.3e-66	57.21	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	1.6e-61	55.79	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	SMART	SM00479	exoiiiendus	coord: 17..173 e-value: 6.5E-10 score: 48.9
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 19..170 e-value: 1.8E-21 score: 77.4
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 10..170 e-value: 4.0E-23 score: 84.2
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 9..170
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 9..170
None	No IPR available	CDD	cd06127	DEDDh	coord: 19..170 e-value: 2.19312E-20 score: 80.8076
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 18..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1849.T4	Cucsat.G1849.T4	mRNA
Cucsat.G1849.T3	Cucsat.G1849.T3	mRNA
Cucsat.G1849.T2	Cucsat.G1849.T2	mRNA
Cucsat.G1849.T9	Cucsat.G1849.T9	mRNA
Cucsat.G1849.T1	Cucsat.G1849.T1	mRNA
Cucsat.G1849.T6	Cucsat.G1849.T6	mRNA
Cucsat.G1849.T5	Cucsat.G1849.T5	mRNA
Cucsat.G1849.T8	Cucsat.G1849.T8	mRNA
Cucsat.G1849.T7	Cucsat.G1849.T7	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding