Cucsat.G17995 (gene) Cucumber (B10) v3

BLAST of Cucsat.G17995 vs. ExPASy Swiss-Prot
Match: Q9ZT82 (Callose synthase 12 OS=Arabidopsis thaliana OX=3702 GN=CALS12 PE=2 SV=1)

HSP 1 Score: 2177.1 bits (5640), Expect = 0.0e+00
Identity = 1087/1414 (76.87%), Postives = 1224/1414 (86.56%), Query Frame = 0

Query: 2    QYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFLI 61
            QY LVSRET     RM+MK I AA W + F V Y  IW Q+ +DR WS  A   +  FL 
Sbjct: 371  QYPLVSRETKRHFFRMLMKVIAAAVWIVAFTVLYTNIWKQKRQDRQWSNAATTKIYQFLY 430

Query: 62   AAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIKY 121
            A G F+ PE+LALALFI+PW+RNF+EETNWK+F+ L+WWFQ ++FVGRGLREGLVDNIKY
Sbjct: 431  AVGAFLVPEILALALFIIPWMRNFLEETNWKIFFALTWWFQGKSFVGRGLREGLVDNIKY 490

Query: 122  SLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPVV 181
            S FWI VLATKF+FSYFLQ+KPM+ P++ L NL DV YEWHQF+  SNRF+V LLWLPVV
Sbjct: 491  STFWIFVLATKFTFSYFLQVKPMIKPSKLLWNLKDVDYEWHQFYGDSNRFSVALLWLPVV 550

Query: 182  LIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLLN 241
            LIYLMD+QIWY+IYSS VGA VGL DHLGEIR+M QLRLRFQFFASAIQFNLMPEEQLLN
Sbjct: 551  LIYLMDIQIWYAIYSSIVGAVVGLFDHLGEIRDMGQLRLRFQFFASAIQFNLMPEEQLLN 610

Query: 242  ARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDRE 301
            ARG   +KFKD IHRLKLRYG G  +KKLESNQVEA KFA+IWNEII  FREEDI+SDRE
Sbjct: 611  ARG-FGNKFKDGIHRLKLRYGFGRPFKKLESNQVEANKFALIWNEIILAFREEDIVSDRE 670

Query: 302  VELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAVI 361
            VELLELP+NSW + VIRWPCFLLCNELLLALSQA+ELIDAPDKWLWHKICKNEYRRCAV+
Sbjct: 671  VELLELPKNSWDVTVIRWPCFLLCNELLLALSQARELIDAPDKWLWHKICKNEYRRCAVV 730

Query: 362  EAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIILA 421
            EAY+SIKHLLL I+K ++EE SI+TV FQ I+ SI  E+FTKTF ++ LP ++  L  L 
Sbjct: 731  EAYDSIKHLLLSIIKVDTEEHSIITVFFQIINQSIQSEQFTKTFRVDLLPKIYETLQKLV 790

Query: 422  ELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENAV 481
             L+N  + D+ +VVN LQ+LYEIATR FF EK+T  QL N+GL  R+  S   LLF+NA+
Sbjct: 791  GLVNDEETDSGRVVNVLQSLYEIATRQFFIEKKTTEQLSNEGLTPRDPASK--LLFQNAI 850

Query: 482  QFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEKM 541
            + PD +NE FYRQVRRLHTILTSRDSMH++P+NLEARRR+AFFSNSLFMN+PHAPQVEKM
Sbjct: 851  RLPDASNEDFYRQVRRLHTILTSRDSMHSVPVNLEARRRIAFFSNSLFMNMPHAPQVEKM 910

Query: 542  MAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDRE 601
            MAFSVLTPYYSEEV+YSKEQLR E EDGIS LYYLQTIY DEWKNF ERMHREG+  D E
Sbjct: 911  MAFSVLTPYYSEEVVYSKEQLRNETEDGISTLYYLQTIYADEWKNFKERMHREGIKTDSE 970

Query: 602  IWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMRR 661
            +WTTKLRDLRLWAS+RGQTL RTVRGMMYYYRALKMLA+LDSASEMDIREG+QEL S+R 
Sbjct: 971  LWTTKLRDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDSASEMDIREGAQELGSVRN 1030

Query: 662  -----EGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKA 721
                  G  DG  S+      SLSR  SSVS L+KGHEYGTALMK+TYVVACQIYG+QKA
Sbjct: 1031 LQGELGGQSDGFVSEND--RSSLSRASSSVSTLYKGHEYGTALMKFTYVVACQIYGSQKA 1090

Query: 722  KKDPHAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPG 781
            KK+P AEEILYLMK NEALR+AYVDEV  GR E +YYSVLVKYDH LEKEVEI+R+KLPG
Sbjct: 1091 KKEPQAEEILYLMKQNEALRIAYVDEVPAGRGETDYYSVLVKYDHQLEKEVEIFRVKLPG 1150

Query: 782  PLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTIL 841
            P+KLGEGKPENQNHA+IFTRGDAVQTIDMNQD+YFEEALKMRNLL+EY   +GIRKPTIL
Sbjct: 1151 PVKLGEGKPENQNHAMIFTRGDAVQTIDMNQDSYFEEALKMRNLLQEYNHYHGIRKPTIL 1210

Query: 842  GVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGI 901
            GVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLK+RMHYGHPDVFDRFWFL+RGGI
Sbjct: 1211 GVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFLSRGGI 1270

Query: 902  SKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQV 961
            SKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQ+SMFEAKVASGNGEQV
Sbjct: 1271 SKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQV 1330

Query: 962  LSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASE 1021
            LSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMV LTVYAFLWGR+YLALSG+E +  ++
Sbjct: 1331 LSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVILTVYAFLWGRVYLALSGVEKSALAD 1390

Query: 1022 S--NNGALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFS 1081
            S   N AL  ILNQQFIIQLGLFTALPMIVE SLE+GFL +IW+F+ MQ+QLS++FYTFS
Sbjct: 1391 STDTNAALGVILNQQFIIQLGLFTALPMIVEWSLEEGFLLAIWNFIRMQIQLSAVFYTFS 1450

Query: 1082 MGTRAHYFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYAS 1141
            MGTRAHYFGRTILHGGAKYRATGRGFVV+HK F ENYRLYARSHF+KAIELGLIL VYAS
Sbjct: 1451 MGTRAHYFGRTILHGGAKYRATGRGFVVEHKGFTENYRLYARSHFVKAIELGLILIVYAS 1510

Query: 1142 HSAVSTNTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIF 1201
            HS ++ ++ +YIAMT TSWFLVISW+MAPFVFNPSGFDWLKTVYDF++FMNWIWY+G I 
Sbjct: 1511 HSPIAKDSLIYIAMTITSWFLVISWIMAPFVFNPSGFDWLKTVYDFEDFMNWIWYQGRIS 1570

Query: 1202 AKAEQSWERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYL 1261
             K+EQSWE+WWYEEQDHL+ TG  G  +E+IL LRFFFFQYG+VYQL I+ GSTS+ VYL
Sbjct: 1571 TKSEQSWEKWWYEEQDHLRNTGKAGLFVEIILVLRFFFFQYGIVYQLKIANGSTSLFVYL 1630

Query: 1262 LSWICVFVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDI 1321
             SWI +F     ++V+ YARD+Y+AK HI YRLVQFL+I+LAI+VIVALLEFT F F DI
Sbjct: 1631 FSWIYIFAIFVLFLVIQYARDKYSAKAHIRYRLVQFLLIVLAILVIVALLEFTHFSFIDI 1690

Query: 1322 FTSLLAFLPTGWGLLLIAQVLRPFLHS-TILWDIVIAVARFYDILFGVIVMIPVAVLSWL 1381
            FTSLLAF+PTGWG+LLIAQ  R +L + TI W+ V++VAR YDILFG+++M+PVA LSW+
Sbjct: 1691 FTSLLAFIPTGWGILLIAQTQRKWLKNYTIFWNAVVSVARMYDILFGILIMVPVAFLSWM 1750

Query: 1382 PGFQSMQTRILFNEAFSRGLRIFQIVTGKKSKVD 1408
            PGFQSMQTRILFNEAFSRGLRI QIVTGKKSK D
Sbjct: 1751 PGFQSMQTRILFNEAFSRGLRIMQIVTGKKSKGD 1779

BLAST of Cucsat.G17995 vs. ExPASy Swiss-Prot
Match: Q9S9U0 (Callose synthase 11 OS=Arabidopsis thaliana OX=3702 GN=CALS11 PE=2 SV=1)

HSP 1 Score: 1987.2 bits (5147), Expect = 0.0e+00
Identity = 982/1406 (69.84%), Postives = 1167/1406 (83.00%), Query Frame = 0

Query: 2    QYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFLI 61
            QYSLVSRET  L +R+ +K +VA AWT+LF VFY RIWSQ+++D VWS  AN+ V  FL 
Sbjct: 362  QYSLVSRETYWLFIRLTLKFVVAVAWTVLFSVFYARIWSQKNKDGVWSRAANERVVTFLK 421

Query: 62   AAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIKY 121
               V++ PE+LAL LFI+P IRN++EE N  V Y L+WWF S+TFVGRG+REGLVDN+KY
Sbjct: 422  VVFVYVIPELLALVLFIVPCIRNWVEELNLGVVYFLTWWFYSKTFVGRGMREGLVDNVKY 481

Query: 122  SLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPVV 181
            +LFWI+VLATKF FSYFLQI+P++APTRALLNL D  Y WH+FF  ++R AV +LWLPV+
Sbjct: 482  TLFWIIVLATKFIFSYFLQIRPLIAPTRALLNLKDATYNWHEFFGSTHRIAVGMLWLPVI 541

Query: 182  LIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLLN 241
            L+YLMDLQIWYSIYSS VGA +GL  HLGEIRN+ QLRLRFQFF+SA+QFNL PEE LL+
Sbjct: 542  LVYLMDLQIWYSIYSSLVGATIGLFSHLGEIRNIDQLRLRFQFFSSAMQFNLKPEEHLLS 601

Query: 242  ARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDRE 301
             + T+  K +DAIHRLKLRYG+G  + K+ES+QVEAT FA+IWNEII  FREED+ISDRE
Sbjct: 602  PKATMLKKARDAIHRLKLRYGIGQPFNKIESSQVEATWFALIWNEIILTFREEDLISDRE 661

Query: 302  VELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAVI 361
            VELLELP N W+I+VIRWPCFLLCNELLLALSQA EL DAPD WLW KIC +EYRRCAV+
Sbjct: 662  VELLELPPNCWNIRVIRWPCFLLCNELLLALSQANELCDAPDHWLWSKICSSEYRRCAVM 721

Query: 362  EAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIILA 421
            EA++SIK ++L+I+K+ +EE+SI+  LF EID ++  EK T+ + +  L  +H KLI L 
Sbjct: 722  EAFDSIKFVILKIVKNGTEEESILNRLFMEIDENVENEKITEVYKLTVLLRIHEKLISLL 781

Query: 422  ELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENAV 481
            E L  P+K   ++VN LQALYE+   +F K +R+  QL   GLA  +  + T LLF NA+
Sbjct: 782  ERLMDPEKKVFRIVNILQALYELCAWEFPKTRRSTPQLRQLGLAPISLEADTELLFVNAI 841

Query: 482  QFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEKM 541
              P + +  FYRQ+RR+HTILTSRD MHN+P N+EAR RLAFFSNSLFM +P AP VEKM
Sbjct: 842  NLPPLDDVVFYRQIRRVHTILTSRDPMHNVPKNIEARERLAFFSNSLFMTMPQAPSVEKM 901

Query: 542  MAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDRE 601
            MAFSVLTPYY EEV+Y +E LR ENEDGIS L+YLQ IY DEW NFLERM REG   + +
Sbjct: 902  MAFSVLTPYYDEEVMYRQEMLRAENEDGISTLFYLQRIYEDEWVNFLERMRREGAENEND 961

Query: 602  IWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMRR 661
            IW+ K+RDLRLWAS+RGQTL+RTVRGMMYYY ALK LA+LDSASEMDIR G+Q     RR
Sbjct: 962  IWSKKVRDLRLWASYRGQTLSRTVRGMMYYYSALKKLAFLDSASEMDIRMGTQIAPEARR 1021

Query: 662  E--GSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKD 721
                +  G  + + TPS+ +SRM S ++ L KG EYG+A+MK+TYVVACQ+YG  KA+ D
Sbjct: 1022 SYYTNDGGDNTLQPTPSQEISRMASGITHLLKGSEYGSAMMKFTYVVACQVYGQHKARGD 1081

Query: 722  PHAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLK 781
              AEEIL+LMK ++ALR+AYVDEV  GR E EYYSVLVK+D  L++EVEIYRI+LPGPLK
Sbjct: 1082 HRAEEILFLMKNHDALRIAYVDEVDLGRGEVEYYSVLVKFDQQLQREVEIYRIRLPGPLK 1141

Query: 782  LGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVR 841
            LGEGKPENQNHA+IFTRGDA+QTIDMNQDN+FEEALKMRNLLE ++  YGIRKPTILGVR
Sbjct: 1142 LGEGKPENQNHALIFTRGDAIQTIDMNQDNHFEEALKMRNLLESFKTYYGIRKPTILGVR 1201

Query: 842  EHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKA 901
            E +FTGSVSSLAWFMSAQETSFVTLGQRVLANPLK+RMHYGHPDVFDRFWF+ RGGISKA
Sbjct: 1202 EKVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRFWFVPRGGISKA 1261

Query: 902  SRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSR 961
            SRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQ+SMFEAKVASGNGEQ LSR
Sbjct: 1262 SRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQISMFEAKVASGNGEQALSR 1321

Query: 962  DVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIAS-ESN 1021
            DVYRLGHRLDFFRMLSFFYTTVG++FNTM++  TVYAFLWGRLYLALSG+E       S+
Sbjct: 1322 DVYRLGHRLDFFRMLSFFYTTVGYYFNTMLIVFTVYAFLWGRLYLALSGVEKIAKDRSSS 1381

Query: 1022 NGALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTR 1081
            N AL  ILNQQFIIQLGLFTALPMI+ENSLE+GFL ++WDF+TMQLQL+S FYTFSMGTR
Sbjct: 1382 NEALGAILNQQFIIQLGLFTALPMILENSLERGFLPAVWDFITMQLQLASFFYTFSMGTR 1441

Query: 1082 AHYFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAV 1141
             HYFGRTILHGGAKYRATGRGFVV+HK FAENYRLYAR+HFIKAIEL +IL VYA++S +
Sbjct: 1442 THYFGRTILHGGAKYRATGRGFVVEHKKFAENYRLYARTHFIKAIELAIILLVYAAYSPL 1501

Query: 1142 STNTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAE 1201
            + ++FVYI MT +SWFL+ SW+++PF+FNPSGFDWLKTV DFD+F+ W+W RG +F KA+
Sbjct: 1502 AKSSFVYILMTISSWFLITSWIISPFLFNPSGFDWLKTVNDFDDFIAWLWSRGGLFTKAD 1561

Query: 1202 QSWERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWI 1261
            QSW  WW EEQ+HLKTTG WGK+LE+ILDLRFFFFQY +VY L I+   TSI VYL+SW 
Sbjct: 1562 QSWFTWWNEEQEHLKTTGVWGKLLEIILDLRFFFFQYSIVYHLRIAENRTSIGVYLISWG 1621

Query: 1262 CVFVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSL 1321
            C+   +A Y+   YA+ RY+ KEHI YR +QFL+I+L ++V+V +L+FT     D+  SL
Sbjct: 1622 CIIGIVAIYITTIYAQKRYSVKEHIKYRFIQFLVILLTVLVVVMMLQFTKLTVVDLLISL 1681

Query: 1322 LAFLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQS 1381
            LAF+PTGWGL+ IAQVL+PFL ST++WD VI+VARFYD+ FG+IVM PVA+LSWLPGFQ+
Sbjct: 1682 LAFVPTGWGLISIAQVLKPFLLSTVVWDTVISVARFYDLFFGLIVMAPVALLSWLPGFQN 1741

Query: 1382 MQTRILFNEAFSRGLRIFQIVTGKKS 1405
            MQTRILFNEAFSRGL+I  I+ GKKS
Sbjct: 1742 MQTRILFNEAFSRGLQISIILAGKKS 1767

BLAST of Cucsat.G17995 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 1285.0 bits (3324), Expect = 0.0e+00
Identity = 693/1416 (48.94%), Postives = 946/1416 (66.81%), Query Frame = 0

Query: 15   VRMIMKSIVAAAWTILFVVFYVRIWS-QRSRDRVWSA---QANKDVGNFLIAAGVFIAPE 74
            +R I+K +V+ AW ++  + Y +  S    + + W +   Q       +++A  +++ P 
Sbjct: 554  LRNILKIVVSLAWCVVLPLCYAQSVSFAPGKLKQWLSFLPQVKGVPPLYIMAVALYLLPN 613

Query: 75   VLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIKYSLFWILVLA 134
            VLA  +FI P +R ++E ++W +F +L WW Q R +VGRG+ E  +  IKY++FW+L+  
Sbjct: 614  VLAAIMFIFPMLRRWIENSDWHIFRLLLWWSQPRIYVGRGMHESQIALIKYTIFWLLLFC 673

Query: 135  TKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGS--NRFAVVLLWLPVVLIYLMDL 194
             KF+FSYFLQ+K ++ PT A++++  V Y+WH+FF  +  N  AVV LWLPV+L+Y MD 
Sbjct: 674  CKFAFSYFLQVKLLVKPTNAIMSIRHVKYKWHEFFPNAEHNYGAVVSLWLPVILVYFMDT 733

Query: 195  QIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLLNARGTLRS 254
            QIWY+I+S+  G  +G  D LGEIR +  LR RFQ    A    L+P ++      +L  
Sbjct: 734  QIWYAIFSTICGGVIGAFDRLGEIRTLGMLRSRFQSLPGAFNTYLVPSDKTRRRGFSLSK 793

Query: 255  KFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDREVELLELP 314
            +F +                   + + EA KF+ +WNEII+ FREED+ISDRE++LL +P
Sbjct: 794  RFAEV----------------TAARRTEAAKFSQLWNEIISSFREEDLISDREMDLLLVP 853

Query: 315  QNS-WSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAVIEAYESI 374
              S  S+K+I+WP FLL +++ +AL  A +     D  LW +IC +EY +CAVIE YES 
Sbjct: 854  YTSDPSLKLIQWPPFLLASKIPIALDMAAQ-FRTRDSDLWKRICADEYMKCAVIECYESF 913

Query: 375  KHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIILAELL--- 434
            KH +L  L     EK I+ ++ +E++ +I+   F   F M  LP L +K + L  +L   
Sbjct: 914  KH-VLHTLVIGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALCSKFVELVGILKNA 973

Query: 435  NKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTST--TGLLFENAVQ 494
            +  K+DT  VV  LQ + E+ TRD  + +    +L+  G   + S      G   + A+ 
Sbjct: 974  DPAKRDT--VVLLLQDMLEVVTRDMMQNE--NRELVELGHTNKESGRQLFAGTDAKPAIL 1033

Query: 495  FPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEKMM 554
            FP V    ++ Q+ RLH +LT ++S  ++P NLEA+RR+AFF+NSLFM++P AP+V  M+
Sbjct: 1034 FPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMPRAPRVRNML 1093

Query: 555  AFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERM--HREGMVIDR 614
            +FSVLTPYYSEE +YSK  L  ENEDG+S++YYLQ I+ DEW NFLER+    E  V++ 
Sbjct: 1094 SFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERLDCKDETSVLES 1153

Query: 615  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 674
            E     +  LR W S RGQTL RTVRGMMYY RALK+ A+LD A+E +I  G + +    
Sbjct: 1154 E---ENILQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEILAGYKAISEPT 1213

Query: 675  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 734
             E        D+ +  RSL     +V           A +K+TYV  CQ YG QK   D 
Sbjct: 1214 EE--------DKKS-QRSLYTQLEAV-----------ADLKFTYVATCQNYGNQKRSGDR 1273

Query: 735  HAEEILYLMKTNEALRVAYVDEVST---GREEKEYYSVLVKYDHVLEKEVEIYRIKLPGP 794
             A +IL LM  N +LRVAY+DEV     G+ +K +YSVL+K    L++  EIYRIKLPGP
Sbjct: 1274 RATDILNLMVNNPSLRVAYIDEVEEREGGKVQKVFYSVLIKAVDNLDQ--EIYRIKLPGP 1333

Query: 795  LKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILG 854
             K+GEGKPENQNHA+IFTRG+A+Q IDMNQD+Y EEALKMRNLLEE+   +G+R PTILG
Sbjct: 1334 AKIGEGKPENQNHALIFTRGEALQAIDMNQDHYLEEALKMRNLLEEFNEDHGVRAPTILG 1393

Query: 855  VREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGIS 914
             REHIFTGSVSSLAWFMS QETSFVT+GQRVLA+PLK+R HYGHPDVFDR + +TRGGIS
Sbjct: 1394 FREHIFTGSVSSLAWFMSNQETSFVTIGQRVLASPLKVRFHYGHPDVFDRIFHITRGGIS 1453

Query: 915  KASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVL 974
            KASR IN+SEDIFAGFN TLR GNVTHHEYIQVGKGRDVGLNQ+S+FEAKVA GNGEQ L
Sbjct: 1454 KASRGINLSEDIFAGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFEAKVACGNGEQTL 1513

Query: 975  SRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTI---A 1034
            SRD+YRLGHR DFFRM+S ++TTVGF+ ++M+V LTVYAFL+GRLYL+LSG+E  I   A
Sbjct: 1514 SRDLYRLGHRFDFFRMMSCYFTTVGFYISSMIVVLTVYAFLYGRLYLSLSGVEEAIVKFA 1573

Query: 1035 SESNNGALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFS 1094
            +   + +L   +  Q ++QLGL   LPM++E  LE+GF  ++ D + MQLQL+ +F+TFS
Sbjct: 1574 AAKGDSSLKAAMASQSVVQLGLLMTLPMVMEIGLERGFRTALSDLIIMQLQLAPVFFTFS 1633

Query: 1095 MGTRAHYFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYAS 1154
            +GT+ HY+GRTILHGG+KYRATGRGFVV+H+ FAENYR+Y+RSHF+K +EL ++L  Y  
Sbjct: 1634 LGTKVHYYGRTILHGGSKYRATGRGFVVKHEKFAENYRMYSRSHFVKGMELMVLLICYRI 1693

Query: 1155 HSAVSTNTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIF 1214
            +   + ++  Y  +  ++WFLV SWL APF FNPSGF+W K V D+D++  WI  RG I 
Sbjct: 1694 YGKAAEDSVGYALVMGSTWFLVGSWLFAPFFFNPSGFEWQKIVDDWDDWNKWISSRGGIG 1753

Query: 1215 AKAEQSWERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGST-----S 1274
              A +SWE WW EEQ+HL  +GF+GK  E+ L LR+F +QYG+VYQL ++  S      S
Sbjct: 1754 VPANKSWESWWEEEQEHLLHSGFFGKFWEIFLSLRYFIYQYGIVYQLNLTKESRMGKQHS 1813

Query: 1275 IAVYLLSWICVFVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAF 1334
            I VY LSW+ +   +    +V+  R +++A   + +RL++  + I ++V++  L  F   
Sbjct: 1814 IIVYGLSWLVIVAVMIVLKIVSMGRKKFSADFQLMFRLLKLFLFIGSVVIVGMLFHFLKL 1873

Query: 1335 KFRDIFTSLLAFLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAV 1394
               DI  SLLAFLPTGW LL I+QV RP + +  +W  V A+AR Y+ + GV++ +PV V
Sbjct: 1874 TVGDIMQSLLAFLPTGWALLQISQVARPLMKTVGMWGSVKALARGYEYIMGVVIFMPVTV 1922

Query: 1395 LSWLPGFQSMQTRILFNEAFSRGLRIFQIVTGKKSK 1406
            L+W P     QTR+LFN+AFSRGL+I +I+ G K +
Sbjct: 1934 LAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGKKQ 1922

BLAST of Cucsat.G17995 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 1237.6 bits (3201), Expect = 0.0e+00
Identity = 673/1439 (46.77%), Postives = 950/1439 (66.02%), Query Frame = 0

Query: 10   TLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQ----RSRDRVWSAQANKDVGNFLIAAGV 69
            TL + +R I+K   AAAW I+  V Y   W          + W   A      F+IA   
Sbjct: 552  TLHVKLRYILKVFSAAAWVIILPVTYAYSWKDPPAFARTIKSWFGSAMHSPSLFIIAVVS 611

Query: 70   FIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIKYSLFW 129
            +++P +LA  +F+ P +R F+E +N+++  ++ WW Q R +VGRG+ E      KY++FW
Sbjct: 612  YLSPNMLAGVMFLFPLLRRFLERSNYRIVMLMMWWSQPRLYVGRGMHESAFSLFKYTMFW 671

Query: 130  ILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFF-RGSNRFAVVL-LWLPVVLI 189
            +L++ATK +FSY+++I+P++APT+A++      ++WH+FF R  N   VV+ LW P++L+
Sbjct: 672  VLLIATKLAFSYYIEIRPLVAPTQAIMKARVTNFQWHEFFPRAKNNIGVVIALWAPIILV 731

Query: 190  YLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLLNAR 249
            Y MD QIWY+I+S+  G   G    LGEIR +  LR RF+    A    L+P+       
Sbjct: 732  YFMDSQIWYAIFSTLFGGIYGAFRRLGEIRTLGMLRSRFESLPGAFNDRLIPD------- 791

Query: 250  GTLRSKFKDAIHRLKLRYGLGHSY---KKLESNQVEATKFAIIWNEIITIFREEDIISDR 309
            G  + K K       +R  L H++   K   + + EA +FA +WN II+ FREED+ISDR
Sbjct: 792  GKNQQKKKG------IRATLSHNFTEDKVPVNKEKEAARFAQLWNTIISSFREEDLISDR 851

Query: 310  EVELLELPQNSWS---IKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRR 369
            E++LL +P   W+   + +I+WP FLL +++ +AL  AK+  +  D+ L  +I  + Y +
Sbjct: 852  EMDLLLVPY--WADRDLDLIQWPPFLLASKIPIALDMAKD-SNGKDRELKKRIESDTYMK 911

Query: 370  CAVIEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKL 429
            CAV E Y S K+++  +++ N  EK ++ ++F E+D  I      + + M+ALP L+   
Sbjct: 912  CAVRECYASFKNIIKFVVQGN-REKEVIEIIFAEVDKHIDTGDLIQEYKMSALPSLYDHF 971

Query: 430  I-ILAELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLL 489
            + ++  LL+  ++D + VV   Q + E+ TRD   E    + L++        T   G++
Sbjct: 972  VKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSS---HGGTWHGGMI 1031

Query: 490  ----------FENAVQFP-DVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFS 549
                         A++FP +   E++  +++R++ +LT+++S  ++P NLEARRR++FFS
Sbjct: 1032 PLEQQYQLFASSGAIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRRISFFS 1091

Query: 550  NSLFMNIPHAPQVEKMMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWK 609
            NSLFM++P AP+V  M++FSVLTPYY+EEVL+S   L T NEDG+SIL+YLQ I+ DEW 
Sbjct: 1092 NSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIFPDEWN 1151

Query: 610  NFLER---MHREGMVIDREIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLD 669
            NFLER   +  E +    E+      +LRLWAS+RGQTLTRTVRGMMYY +AL++ A+LD
Sbjct: 1152 NFLERVKCLSEEELKESDEL----EEELRLWASYRGQTLTRTVRGMMYYRKALELQAFLD 1211

Query: 670  SASEMDIREGSQELDSMRREGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKY 729
             A   D+ EG +         +++  + + S   RSL     +V           A MK+
Sbjct: 1212 MAMHEDLMEGYK---------AVELNSENNSRGERSLWAQCQAV-----------ADMKF 1271

Query: 730  TYVVACQIYGTQKAKKDPHAEEILYLMKTNEALRVAYVDEV-------STGREEKEYYSV 789
            TYVV+CQ YG  K   DP A++IL LM    +LRVAY+DEV       S    +K YYSV
Sbjct: 1272 TYVVSCQQYGIHKRSGDPRAQDILRLMTRYPSLRVAYIDEVEEPVKDKSKKGNQKVYYSV 1331

Query: 790  LVKY----DH-VLEKEVE--IYRIKLPGPLKLGEGKPENQNHAIIFTRGDAVQTIDMNQD 849
            LVK     DH  L + ++  IYRI+LPGP  LGEGKPENQNHAIIF+RG+ +QTIDMNQD
Sbjct: 1332 LVKVPKSTDHSTLAQNLDQVIYRIRLPGPAILGEGKPENQNHAIIFSRGEGLQTIDMNQD 1391

Query: 850  NYFEEALKMRNLLEEYRRSY-GIRKPTILGVREHIFTGSVSSLAWFMSAQETSFVTLGQR 909
            NY EEALKMRNLL+E+   + G+R P+ILG+REHIFTGSVSSLAWFMS QETSFVT+GQR
Sbjct: 1392 NYMEEALKMRNLLQEFLTKHDGVRHPSILGLREHIFTGSVSSLAWFMSNQETSFVTIGQR 1451

Query: 910  VLANPLKIRMHYGHPDVFDRFWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEY 969
            +LANPL++R HYGHPDVFDR + LTRGG+SKAS+VIN+SEDIFAGFN TLR GNVTHHEY
Sbjct: 1452 LLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHHEY 1511

Query: 970  IQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRDVYRLGHRLDFFRMLSFFYTTVGFFFNT 1029
            IQVGKGRDVGLNQ+SMFEAK+A+GNGEQ LSRD+YRLGHR DFFRM+S ++TTVGF+F+T
Sbjct: 1512 IQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYFST 1571

Query: 1030 MMVTLTVYAFLWGRLYLALSGIENTIASES---NNGALATILNQQFIIQLGLFTALPMIV 1089
            ++  LTVY FL+GRLYL LSG+E  ++++    +N  L   L  Q  +Q+G   ALPM++
Sbjct: 1572 LITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPMLM 1631

Query: 1090 ENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAHYFGRTILHGGAKYRATGRGFVVQH 1149
            E  LE+GF  ++ +F+ MQLQL+ +F+TFS+GT+ HY+GRT+LHGGAKYR+TGRGFVV H
Sbjct: 1632 EIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVVFH 1691

Query: 1150 KSFAENYRLYARSHFIKAIELGLILTVYASHSAVSTNTFVYIAMTFTSWFLVISWLMAPF 1209
              FA+NYRLY+RSHF+K +E+ L+L VY    +       Y+ +T + WF+V +WL APF
Sbjct: 1692 AKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFAPF 1751

Query: 1210 VFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQSWERWWYEEQDHLKTTGFWGKVLEV 1269
            +FNPSGF+W K V D+ ++  WI   G I   AE+SWE WW EEQ+HL+ +G  G V+E+
Sbjct: 1752 LFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVVEI 1811

Query: 1270 ILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICVFVALATYVVVAYARDRYAAKEHIY 1329
            +L LRFF +QYG+VY L I+  + +  VY +SW+ +F+ L     V+  R R++A   + 
Sbjct: 1812 LLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRRFSASFQLM 1871

Query: 1330 YRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLAFLPTGWGLLLIAQVLRPFLHSTIL 1389
            +RL++ LI +  I +IV L+       +DI   +LAF+PTGWG+LLIAQ  +P +H    
Sbjct: 1872 FRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACKPVVHRAGF 1931

Query: 1390 WDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQTRILFNEAFSRGLRIFQIVTGKK 1404
            W  V  +AR Y+I+ G+++  PVA L+W P     QTR+LFN+AFSRGL+I +I+ G +
Sbjct: 1932 WGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGHR 1946

BLAST of Cucsat.G17995 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 657/1433 (45.85%), Postives = 926/1433 (64.62%), Query Frame = 0

Query: 10   TLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSR-----DRVWSAQANKDVGNFLIAAG 69
            +L + +R +MK   AA W ++  V Y   W   S         +   ++     F++A  
Sbjct: 546  SLYVKLRYVMKVGAAAVWVVVMAVTYAYSWKNASGFSQTIKNWFGGHSHNSPSLFIVAIL 605

Query: 70   VFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIKYSLF 129
            ++++P +L+  LF+ P+IR ++E +++K+  ++ WW Q R ++GRG+ E  +   KY++F
Sbjct: 606  IYLSPNMLSALLFLFPFIRRYLERSDYKIMMLMMWWSQPRLYIGRGMHESALSLFKYTMF 665

Query: 130  WILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFF--RGSNRFAVVLLWLPVVL 189
            WI++L +K +FSY+ +IKP++ PT+ ++ +    Y WH+FF    +N   V+ LW PV+L
Sbjct: 666  WIVLLISKLAFSYYAEIKPLVGPTKDIMRIHISVYSWHEFFPHAKNNLGVVIALWSPVIL 725

Query: 190  IYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLLNA 249
            +Y MD QIWY+I S+ VG   G    LGEIR +  LR RFQ    A    L+P++   N+
Sbjct: 726  VYFMDTQIWYAIVSTLVGGLNGAFRRLGEIRTLGMLRSRFQSIPGAFNDCLVPQD---NS 785

Query: 250  RGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQ-VEATKFAIIWNEIITIFREEDIISDRE 309
              T + +F+    R          + +L S++  EA +FA +WN+II+ FREED+ISDRE
Sbjct: 786  DDTKKKRFRATFSR---------KFDQLPSSKDKEAARFAQMWNKIISSFREEDLISDRE 845

Query: 310  VELLELPQNSWS---IKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRC 369
            +ELL +P   WS   + +IRWP FLL +++ +AL  AK+  +  D+ L  ++  + Y  C
Sbjct: 846  MELLLVPY--WSDPDLDLIRWPPFLLASKIPIALDMAKD-SNGKDRELKKRLAVDSYMTC 905

Query: 370  AVIEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLI 429
            AV E Y S K+ L+  L     E  ++  +F +ID  I  E      N++ALPDL+ + +
Sbjct: 906  AVRECYASFKN-LINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYGQFV 965

Query: 430  ILAE-LLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG-AQLINDGLALRNSTST---T 489
             L E LL   ++D +Q+V  L  + E+ TRD  +E+     +  ++G  ++    T    
Sbjct: 966  RLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTPLHQ 1025

Query: 490  GLLFENAVQFPDVT-NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNI 549
               + + ++FP  +  E++  +++RLH +LT ++S  ++P NLEARRRL FFSNSLFM++
Sbjct: 1026 QRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLFMDM 1085

Query: 550  PHAPQVEKMMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMH 609
            P AP++  M++FSVLTPY+SE+VL+S   L  +NEDG+SIL+YLQ I+ DEW NFLER+ 
Sbjct: 1086 PPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLERV- 1145

Query: 610  REGMVIDREIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREG 669
            + G   +         +LRLWAS+RGQTLT+TVRGMMYY +AL++ A+LD A + ++ +G
Sbjct: 1146 KCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELLKG 1205

Query: 670  SQELDSMRREGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYG 729
             + L+    E S  G              + +    L        A MK+T+VV+CQ Y 
Sbjct: 1206 YKALELTSEEASKSG------------GSLWAQCQAL--------ADMKFTFVVSCQQYS 1265

Query: 730  TQKAKKDPHAEEILYLMKTNEALRVAYVDEVS-------TGREEKEYYSVLVK------- 789
              K   D  A++IL LM T  ++RVAY+DEV         G EEK YYS LVK       
Sbjct: 1266 IHKRSGDQRAKDILRLMTTYPSIRVAYIDEVEQTHKESYKGTEEKIYYSALVKAAPQTKP 1325

Query: 790  ---YDHVLEKEVEIYRIKLPGPLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEAL 849
                + V   +  IYRIKLPGP  LGEGKPENQNHAIIFTRG+ +QTIDMNQDNY EEA 
Sbjct: 1326 MDSSESVQTLDQLIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEAF 1385

Query: 850  KMRNLLEEYRRSY-GIRKPTILGVREHIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLK 909
            KMRNLL+E+   + G+R PTILG+REHIFTGSVSSLAWFMS QE SFVT+GQRVLA+PLK
Sbjct: 1386 KMRNLLQEFLEKHGGVRCPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLK 1445

Query: 910  IRMHYGHPDVFDRFWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGR 969
            +R HYGHPD+FDR + LTRGGI KAS+VIN+SEDIFAGFN TLR GNVTHHEYIQVGKGR
Sbjct: 1446 VRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGR 1505

Query: 970  DVGLNQVSMFEAKVASGNGEQVLSRDVYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTV 1029
            DVGLNQ+SMFEAK+A+GNGEQ LSRD+YRLGHR DFFRMLS ++TT+GF+F+TM+  LTV
Sbjct: 1506 DVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTV 1565

Query: 1030 YAFLWGRLYLALSGIENTIASE---SNNGALATILNQQFIIQLGLFTALPMIVENSLEQG 1089
            Y FL+GRLYL LSG+E  ++S+    NN  L   L  Q  +Q+G   ALPM++E  LE+G
Sbjct: 1566 YVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPMMMEIGLERG 1625

Query: 1090 FLQSIWDFLTMQLQLSSIFYTFSMGTRAHYFGRTILHGGAKYRATGRGFVVQHKSFAENY 1149
            F  ++ +F+ MQLQL+S+F+TF +GT+ HY+GRT+ HGGA+YR TGRGFVV H  FAENY
Sbjct: 1626 FHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENY 1685

Query: 1150 RLYARSHFIKAIELGLILTVYASHSAVSTNTFVYIAMTFTSWFLVISWLMAPFVFNPSGF 1209
            R Y+RSHF+K IEL ++L VY            YI +T + WF+V++WL APF+FNPSGF
Sbjct: 1686 RFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGF 1745

Query: 1210 DWLKTVYDFDEFMNWIWYRGSIFAKAEQSWERWWYEEQDHLKTTGFWGKVLEVILDLRFF 1269
            +W K V D+ ++  WI+ RG I    E+SWE WW +E +HL+ +G  G  LE+ L LRFF
Sbjct: 1746 EWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITLEIFLALRFF 1805

Query: 1270 FFQYGVVYQLGISAG-STSIAVYLLSWICVFVALATYVVVAYARDRYAAKEHIYYRLVQF 1329
             FQYG+VY L    G + S  VY  SW  +   L     +   R R++    + +R+++ 
Sbjct: 1806 IFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKG 1865

Query: 1330 LIIILAIVVIVALLEFTAFKFRDIFTSLLAFLPTGWGLLLIAQVLRPFLHSTILWDIVIA 1389
            L+ +  + +++  L       +D+F  +LAF+PTGWG+LLIAQ  +P +    +W  V  
Sbjct: 1866 LVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRT 1925

Query: 1390 VARFYDILFGVIVMIPVAVLSWLPGFQSMQTRILFNEAFSRGLRIFQIVTGKK 1404
            +AR Y+I+ G+++  PVA L+W P     QTR+LFN+AFSRGL+I +I+ G++
Sbjct: 1926 LARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGQR 1941

BLAST of Cucsat.G17995 vs. NCBI nr
Match: XP_004140034.1 (callose synthase 12 [Cucumis sativus] >KGN46739.1 hypothetical protein Csa_020960 [Cucumis sativus])

HSP 1 Score: 2767 bits (7172), Expect = 0.0
Identity = 1406/1409 (99.79%), Postives = 1408/1409 (99.93%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRS+DRVWSAQANKDVGNFL
Sbjct: 359  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSQDRVWSAQANKDVGNFL 418

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 419  IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 478

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV
Sbjct: 479  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 538

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 539  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 598

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 599  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 658

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 659  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 718

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL
Sbjct: 719  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 778

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG QLINDGLALRNSTSTTGLLFENA
Sbjct: 779  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGDQLINDGLALRNSTSTTGLLFENA 838

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 839  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 898

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR
Sbjct: 899  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 958

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR
Sbjct: 959  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 1018

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1019 REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1078

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL
Sbjct: 1079 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 1138

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRR+YGIRKPTILGVRE
Sbjct: 1139 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRNYGIRKPTILGVRE 1198

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1199 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1258

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD
Sbjct: 1259 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 1318

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG
Sbjct: 1319 VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1378

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1379 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1438

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST
Sbjct: 1439 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1498

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS
Sbjct: 1499 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1558

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV
Sbjct: 1559 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1618

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA
Sbjct: 1619 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1678

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1679 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1738

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1739 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1767

BLAST of Cucsat.G17995 vs. NCBI nr
Match: XP_008448292.1 (PREDICTED: callose synthase 12-like [Cucumis melo] >KAA0062056.1 callose synthase 12-like [Cucumis melo var. makuwa] >TYK04965.1 callose synthase 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 2736 bits (7093), Expect = 0.0
Identity = 1388/1409 (98.51%), Postives = 1399/1409 (99.29%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL
Sbjct: 359  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 418

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALF+LPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 419  IAAGVFIAPEVLALALFLLPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 478

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPMM PTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV
Sbjct: 479  YSLFWILVLATKFSFSYFLQIKPMMGPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 538

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 539  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 598

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 599  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 658

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 659  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 718

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSI IEKFTKTFNMNALPDLHAKLI L
Sbjct: 719  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPDLHAKLIKL 778

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG QLI DGLALRNSTSTTGLLFENA
Sbjct: 779  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGDQLIIDGLALRNSTSTTGLLFENA 838

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            +QFPDV+NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 839  IQFPDVSNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 898

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMV D 
Sbjct: 899  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVEDG 958

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL+SMR
Sbjct: 959  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELNSMR 1018

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1019 REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1078

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL
Sbjct: 1079 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 1138

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRR+YGIRKPTILGVRE
Sbjct: 1139 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRNYGIRKPTILGVRE 1198

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1199 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1258

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD
Sbjct: 1259 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 1318

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNT MV LTVYAFLWGRLYLALSGIEN IASESNNG
Sbjct: 1319 VYRLGHRLDFFRMLSFFYTTVGFFFNTTMVILTVYAFLWGRLYLALSGIENAIASESNNG 1378

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1379 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1438

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVS+
Sbjct: 1439 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVSS 1498

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNW+WYRGSIFAKAEQS
Sbjct: 1499 DTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWVWYRGSIFAKAEQS 1558

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYG+VYQLGISAGSTSIAVYLLSWICV
Sbjct: 1559 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGIVYQLGISAGSTSIAVYLLSWICV 1618

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRD+FTSLLA
Sbjct: 1619 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDVFTSLLA 1678

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1679 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1738

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1739 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1767

BLAST of Cucsat.G17995 vs. NCBI nr
Match: XP_038901872.1 (callose synthase 12-like [Benincasa hispida])

HSP 1 Score: 2714 bits (7036), Expect = 0.0
Identity = 1374/1409 (97.52%), Postives = 1394/1409 (98.94%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWS QANKDVGNFL
Sbjct: 358  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSTQANKDVGNFL 417

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALFILPWIRNFMEETNWK+FYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 418  IAAGVFIAPEVLALALFILPWIRNFMEETNWKIFYMLSWWFQSRTFVGRGLREGLVDNIK 477

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKF+FSYFLQIKPMM PTRALLNLGDV YEWHQFFRGSNRFAVVLLWLPV
Sbjct: 478  YSLFWILVLATKFAFSYFLQIKPMMGPTRALLNLGDVTYEWHQFFRGSNRFAVVLLWLPV 537

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGL DHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 538  VLIYLMDLQIWYSIYSSFVGAAVGLFDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 597

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTL+SKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 598  NARGTLKSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 657

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSW+IKVIRWPCFLLCNELLLALSQAKEL+DAPDKWLWHKICKNEYRRCAV
Sbjct: 658  EVELLELPQNSWNIKVIRWPCFLLCNELLLALSQAKELVDAPDKWLWHKICKNEYRRCAV 717

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSI IEKFTKTFNMNALP+LH+KLI L
Sbjct: 718  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPELHSKLIKL 777

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEI+TRDFFKEKRTG QLI DGLALRNSTSTTGLLFENA
Sbjct: 778  AELLNKPKKDTNQVVNTLQALYEISTRDFFKEKRTGDQLIIDGLALRNSTSTTGLLFENA 837

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            V+FPD +NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 838  VKFPDASNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 897

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMV D 
Sbjct: 898  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVKDS 957

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL+SMR
Sbjct: 958  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELNSMR 1017

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDG ASDRS+PSRSLSR GSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1018 REGSIDGSASDRSSPSRSLSRKGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1077

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDH LEKEVEIYRIKLPGPLKL
Sbjct: 1078 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHQLEKEVEIYRIKLPGPLKL 1137

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYR +YGIRKPTILGVRE
Sbjct: 1138 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRHNYGIRKPTILGVRE 1197

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1198 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1257

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQ+LSRD
Sbjct: 1258 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQILSRD 1317

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIEN+IASESNNG
Sbjct: 1318 VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENSIASESNNG 1377

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1378 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1437

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVS+
Sbjct: 1438 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVSS 1497

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS
Sbjct: 1498 DTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1557

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYG+VYQLGISAGSTSIAVYLLSWICV
Sbjct: 1558 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGIVYQLGISAGSTSIAVYLLSWICV 1617

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILAIVVIVALLEFT+FKFRDIFTSLLA
Sbjct: 1618 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAIVVIVALLEFTSFKFRDIFTSLLA 1677

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1678 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1737

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1738 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1766

BLAST of Cucsat.G17995 vs. NCBI nr
Match: XP_022970702.1 (callose synthase 12-like [Cucurbita maxima])

HSP 1 Score: 2628 bits (6812), Expect = 0.0
Identity = 1325/1409 (94.04%), Postives = 1370/1409 (97.23%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVR++MKSIVAAAWTILFVVFYVRIW+QR+RDR+WSAQANKDV NFL
Sbjct: 354  MQYSLVSRETLGLGVRLVMKSIVAAAWTILFVVFYVRIWTQRNRDRIWSAQANKDVSNFL 413

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IA GVFI PEVLALALFILPWIRNFMEETNW++FYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 414  IAGGVFIVPEVLALALFILPWIRNFMEETNWRIFYMLSWWFQSRTFVGRGLREGLVDNIK 473

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPM+APT++LL L DV YEWHQFF  SNRFAV+LLWLPV
Sbjct: 474  YSLFWILVLATKFSFSYFLQIKPMIAPTKSLLQLRDVTYEWHQFFTRSNRFAVILLWLPV 533

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGL DHLGEIRN+PQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 534  VLIYLMDLQIWYSIYSSFVGAAVGLFDHLGEIRNIPQLRLRFQFFASAIQFNLMPEEQLL 593

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTL+SKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISD 
Sbjct: 594  NARGTLKSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDH 653

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELP NSW+IKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 654  EVELLELPLNSWNIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 713

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESI+HL+LQILKHNSEEKSIMTVLFQEIDHSI +EKFTKTFNMNALP+LH KLI L
Sbjct: 714  IEAYESIRHLILQILKHNSEEKSIMTVLFQEIDHSIELEKFTKTFNMNALPELHTKLIKL 773

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELL+KPKKDTNQVVNTLQA+YEIATRDFFKEKR+G QLI DGLALRNSTSTTGLLFENA
Sbjct: 774  AELLSKPKKDTNQVVNTLQAIYEIATRDFFKEKRSGDQLIVDGLALRNSTSTTGLLFENA 833

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            V+FPD +NESFYRQ RRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 834  VKFPDASNESFYRQARRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 893

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERM+REGM+ D 
Sbjct: 894  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMNREGMMKDN 953

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASE+DIREGSQEL S+R
Sbjct: 954  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEVDIREGSQELGSLR 1013

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            +EGSIDG AS+    SR+ SR GSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1014 QEGSIDGFASESLPSSRNFSRSGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1073

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMK+NEALRVAYVDEVSTGR EK+YYSVLVKYDH LE+EVEIYRIKLPGPLKL
Sbjct: 1074 HAEEILYLMKSNEALRVAYVDEVSTGRGEKDYYSVLVKYDHQLEREVEIYRIKLPGPLKL 1133

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYR +YGIRKPTILGVRE
Sbjct: 1134 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRHNYGIRKPTILGVRE 1193

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1194 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1253

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQVSMFEAKVASGNGEQVLSRD
Sbjct: 1254 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQVSMFEAKVASGNGEQVLSRD 1313

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIEN IASESNNG
Sbjct: 1314 VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENAIASESNNG 1373

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLE GFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1374 ALATILNQQFIIQLGLFTALPMIVENSLEHGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1433

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLIL VYASHSAVS+
Sbjct: 1434 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILIVYASHSAVSS 1493

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLV SWLMAPFVFNPSGFDWLKTVYDFD+FMNWIWYRGSIFAKAEQS
Sbjct: 1494 DTFVYIAMTFTSWFLVGSWLMAPFVFNPSGFDWLKTVYDFDDFMNWIWYRGSIFAKAEQS 1553

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLE+ILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWI V
Sbjct: 1554 WERWWYEEQDHLKTTGFWGKVLEIILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWIYV 1613

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILA+V+IVALLEFTAF+F DIFTSLLA
Sbjct: 1614 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAVVIIVALLEFTAFRFIDIFTSLLA 1673

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFL STILW++VI+VARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1674 FLPTGWGLLLIAQVLRPFLQSTILWEVVISVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1733

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1734 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1762

BLAST of Cucsat.G17995 vs. NCBI nr
Match: XP_022985898.1 (callose synthase 12-like [Cucurbita maxima])

HSP 1 Score: 2627 bits (6810), Expect = 0.0
Identity = 1327/1409 (94.18%), Postives = 1366/1409 (96.95%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLG  VRMIMKSIVAAAWT+LF+VFY RIWSQRS+DR WSAQA+KD+GNFL
Sbjct: 358  MQYSLVSRETLGPAVRMIMKSIVAAAWTVLFIVFYWRIWSQRSQDRAWSAQADKDIGNFL 417

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALFILPWIRNFMEETNW++FYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 418  IAAGVFIAPEVLALALFILPWIRNFMEETNWRIFYMLSWWFQSRTFVGRGLREGLVDNIK 477

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YS+FWILVLA+KFSFSYFLQIKPMM PT+ALL LGDV YEWHQFFRGSNRFAV+LLWLPV
Sbjct: 478  YSMFWILVLASKFSFSYFLQIKPMMGPTKALLKLGDVSYEWHQFFRGSNRFAVLLLWLPV 537

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            +LIYLMDLQIWYSIYSSFVGAAVGL DHLGEIRN+PQLRLRFQFFASAIQFNLMP+EQLL
Sbjct: 538  LLIYLMDLQIWYSIYSSFVGAAVGLFDHLGEIRNIPQLRLRFQFFASAIQFNLMPQEQLL 597

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTL+SKFKDAIHRLKLRYGLGHSY KLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 598  NARGTLKSKFKDAIHRLKLRYGLGHSYNKLESNQVEATKFAIIWNEIITIFREEDIISDR 657

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELP NSWSIKV+RWPCFLLCNELLL+LSQAKEL DA DKWLWHKICKNEYRRCAV
Sbjct: 658  EVELLELPLNSWSIKVVRWPCFLLCNELLLSLSQAKELTDATDKWLWHKICKNEYRRCAV 717

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHN+EEKSIMTVLFQEIDHSI IEKFTKTFNMNALPDLH KLI L
Sbjct: 718  IEAYESIKHLLLQILKHNTEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPDLHTKLIKL 777

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELL KPKKDT  VVNTLQA+YEI TRDFFKEKR+G QLI DGLALRNSTSTTGLLFENA
Sbjct: 778  AELLTKPKKDTIGVVNTLQAVYEITTRDFFKEKRSGDQLIIDGLALRNSTSTTGLLFENA 837

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            V+FPDV NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 838  VKFPDVGNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 897

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERM REGM  D 
Sbjct: 898  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMRREGMGKDS 957

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL SM 
Sbjct: 958  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELGSMG 1017

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            +EGSID +ASDRS  +RSLSR GSSVS LFKGHEYGTALMKYTYVVACQIYG+QKAKKDP
Sbjct: 1018 QEGSIDSVASDRSLQTRSLSRTGSSVSFLFKGHEYGTALMKYTYVVACQIYGSQKAKKDP 1077

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMK+NEALRVAYVDEV TGREEKEYYSVLVKYDH LEKEVEIYRIKLPGPLKL
Sbjct: 1078 HAEEILYLMKSNEALRVAYVDEVCTGREEKEYYSVLVKYDHQLEKEVEIYRIKLPGPLKL 1137

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEE++RSYGIRKPTILGVRE
Sbjct: 1138 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEFKRSYGIRKPTILGVRE 1197

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPD+FDRFWFLTRGGISKAS
Sbjct: 1198 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDMFDRFWFLTRGGISKAS 1257

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQVSMFEAKVASGNGEQVLSRD
Sbjct: 1258 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQVSMFEAKVASGNGEQVLSRD 1317

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFY+TVGFFFNTMMVTLTVY FLWGRLYLALSGIEN IA ESNNG
Sbjct: 1318 VYRLGHRLDFFRMLSFFYSTVGFFFNTMMVTLTVYVFLWGRLYLALSGIENEIARESNNG 1377

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLE GFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1378 ALATILNQQFIIQLGLFTALPMIVENSLEHGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1437

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLIL VYASH+AVS+
Sbjct: 1438 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILIVYASHNAVSS 1497

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            NTFVYIAMTFTSWFLV+SWLMAPFVFNPSGFDWLKTVYDFD+FMNWIWYRGSIFAKAEQS
Sbjct: 1498 NTFVYIAMTFTSWFLVLSWLMAPFVFNPSGFDWLKTVYDFDDFMNWIWYRGSIFAKAEQS 1557

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGS SIAVYLLSWICV
Sbjct: 1558 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSKSIAVYLLSWICV 1617

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYAR++YAAK+HIYYRLVQFLIIILAIVVIVALLEFTAF+FRDIFTSLLA
Sbjct: 1618 FVALATYVVVAYARNKYAAKDHIYYRLVQFLIIILAIVVIVALLEFTAFEFRDIFTSLLA 1677

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWG LLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVM+PVAVLSWLPGFQSMQ
Sbjct: 1678 FLPTGWGFLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMVPVAVLSWLPGFQSMQ 1737

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1738 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1766

BLAST of Cucsat.G17995 vs. ExPASy TrEMBL
Match: A0A0A0KD16 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_6G128000 PE=3 SV=1)

HSP 1 Score: 2767 bits (7172), Expect = 0.0
Identity = 1406/1409 (99.79%), Postives = 1408/1409 (99.93%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRS+DRVWSAQANKDVGNFL
Sbjct: 359  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSQDRVWSAQANKDVGNFL 418

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 419  IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 478

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV
Sbjct: 479  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 538

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 539  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 598

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 599  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 658

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 659  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 718

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL
Sbjct: 719  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 778

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG QLINDGLALRNSTSTTGLLFENA
Sbjct: 779  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGDQLINDGLALRNSTSTTGLLFENA 838

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 839  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 898

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR
Sbjct: 899  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 958

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR
Sbjct: 959  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 1018

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1019 REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1078

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL
Sbjct: 1079 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 1138

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRR+YGIRKPTILGVRE
Sbjct: 1139 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRNYGIRKPTILGVRE 1198

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1199 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1258

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD
Sbjct: 1259 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 1318

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG
Sbjct: 1319 VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1378

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1379 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1438

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST
Sbjct: 1439 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1498

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS
Sbjct: 1499 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1558

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV
Sbjct: 1559 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1618

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA
Sbjct: 1619 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1678

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1679 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1738

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1739 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1767

BLAST of Cucsat.G17995 vs. ExPASy TrEMBL
Match: A0A5D3C0T1 (1,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001840 PE=3 SV=1)

HSP 1 Score: 2736 bits (7093), Expect = 0.0
Identity = 1388/1409 (98.51%), Postives = 1399/1409 (99.29%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL
Sbjct: 359  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 418

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALF+LPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 419  IAAGVFIAPEVLALALFLLPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 478

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPMM PTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV
Sbjct: 479  YSLFWILVLATKFSFSYFLQIKPMMGPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 538

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 539  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 598

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 599  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 658

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 659  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 718

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSI IEKFTKTFNMNALPDLHAKLI L
Sbjct: 719  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPDLHAKLIKL 778

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG QLI DGLALRNSTSTTGLLFENA
Sbjct: 779  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGDQLIIDGLALRNSTSTTGLLFENA 838

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            +QFPDV+NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 839  IQFPDVSNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 898

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMV D 
Sbjct: 899  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVEDG 958

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL+SMR
Sbjct: 959  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELNSMR 1018

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1019 REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1078

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL
Sbjct: 1079 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 1138

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRR+YGIRKPTILGVRE
Sbjct: 1139 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRNYGIRKPTILGVRE 1198

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1199 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1258

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD
Sbjct: 1259 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 1318

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNT MV LTVYAFLWGRLYLALSGIEN IASESNNG
Sbjct: 1319 VYRLGHRLDFFRMLSFFYTTVGFFFNTTMVILTVYAFLWGRLYLALSGIENAIASESNNG 1378

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1379 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1438

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVS+
Sbjct: 1439 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVSS 1498

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNW+WYRGSIFAKAEQS
Sbjct: 1499 DTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWVWYRGSIFAKAEQS 1558

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYG+VYQLGISAGSTSIAVYLLSWICV
Sbjct: 1559 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGIVYQLGISAGSTSIAVYLLSWICV 1618

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRD+FTSLLA
Sbjct: 1619 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDVFTSLLA 1678

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1679 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1738

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1739 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1767

BLAST of Cucsat.G17995 vs. ExPASy TrEMBL
Match: A0A1S3BJD1 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103490530 PE=3 SV=1)

HSP 1 Score: 2736 bits (7093), Expect = 0.0
Identity = 1388/1409 (98.51%), Postives = 1399/1409 (99.29%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL
Sbjct: 359  MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 418

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALF+LPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 419  IAAGVFIAPEVLALALFLLPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 478

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPMM PTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV
Sbjct: 479  YSLFWILVLATKFSFSYFLQIKPMMGPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 538

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 539  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 598

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 599  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 658

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 659  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 718

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSI IEKFTKTFNMNALPDLHAKLI L
Sbjct: 719  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPDLHAKLIKL 778

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTG QLI DGLALRNSTSTTGLLFENA
Sbjct: 779  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGDQLIIDGLALRNSTSTTGLLFENA 838

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            +QFPDV+NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 839  IQFPDVSNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 898

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMV D 
Sbjct: 899  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVEDG 958

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL+SMR
Sbjct: 959  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELNSMR 1018

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1019 REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1078

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL
Sbjct: 1079 HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 1138

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRR+YGIRKPTILGVRE
Sbjct: 1139 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRNYGIRKPTILGVRE 1198

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1199 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1258

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD
Sbjct: 1259 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 1318

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNT MV LTVYAFLWGRLYLALSGIEN IASESNNG
Sbjct: 1319 VYRLGHRLDFFRMLSFFYTTVGFFFNTTMVILTVYAFLWGRLYLALSGIENAIASESNNG 1378

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1379 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1438

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVS+
Sbjct: 1439 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVSS 1498

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNW+WYRGSIFAKAEQS
Sbjct: 1499 DTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWVWYRGSIFAKAEQS 1558

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYG+VYQLGISAGSTSIAVYLLSWICV
Sbjct: 1559 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGIVYQLGISAGSTSIAVYLLSWICV 1618

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRD+FTSLLA
Sbjct: 1619 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDVFTSLLA 1678

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1679 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1738

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1739 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1767

BLAST of Cucsat.G17995 vs. ExPASy TrEMBL
Match: A0A6J1HZV7 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111469606 PE=3 SV=1)

HSP 1 Score: 2628 bits (6812), Expect = 0.0
Identity = 1325/1409 (94.04%), Postives = 1370/1409 (97.23%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLGLGVR++MKSIVAAAWTILFVVFYVRIW+QR+RDR+WSAQANKDV NFL
Sbjct: 354  MQYSLVSRETLGLGVRLVMKSIVAAAWTILFVVFYVRIWTQRNRDRIWSAQANKDVSNFL 413

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IA GVFI PEVLALALFILPWIRNFMEETNW++FYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 414  IAGGVFIVPEVLALALFILPWIRNFMEETNWRIFYMLSWWFQSRTFVGRGLREGLVDNIK 473

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YSLFWILVLATKFSFSYFLQIKPM+APT++LL L DV YEWHQFF  SNRFAV+LLWLPV
Sbjct: 474  YSLFWILVLATKFSFSYFLQIKPMIAPTKSLLQLRDVTYEWHQFFTRSNRFAVILLWLPV 533

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            VLIYLMDLQIWYSIYSSFVGAAVGL DHLGEIRN+PQLRLRFQFFASAIQFNLMPEEQLL
Sbjct: 534  VLIYLMDLQIWYSIYSSFVGAAVGLFDHLGEIRNIPQLRLRFQFFASAIQFNLMPEEQLL 593

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTL+SKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISD 
Sbjct: 594  NARGTLKSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDH 653

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELP NSW+IKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV
Sbjct: 654  EVELLELPLNSWNIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 713

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESI+HL+LQILKHNSEEKSIMTVLFQEIDHSI +EKFTKTFNMNALP+LH KLI L
Sbjct: 714  IEAYESIRHLILQILKHNSEEKSIMTVLFQEIDHSIELEKFTKTFNMNALPELHTKLIKL 773

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELL+KPKKDTNQVVNTLQA+YEIATRDFFKEKR+G QLI DGLALRNSTSTTGLLFENA
Sbjct: 774  AELLSKPKKDTNQVVNTLQAIYEIATRDFFKEKRSGDQLIVDGLALRNSTSTTGLLFENA 833

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            V+FPD +NESFYRQ RRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 834  VKFPDASNESFYRQARRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 893

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERM+REGM+ D 
Sbjct: 894  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMNREGMMKDN 953

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASE+DIREGSQEL S+R
Sbjct: 954  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEVDIREGSQELGSLR 1013

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            +EGSIDG AS+    SR+ SR GSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP
Sbjct: 1014 QEGSIDGFASESLPSSRNFSRSGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 1073

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMK+NEALRVAYVDEVSTGR EK+YYSVLVKYDH LE+EVEIYRIKLPGPLKL
Sbjct: 1074 HAEEILYLMKSNEALRVAYVDEVSTGRGEKDYYSVLVKYDHQLEREVEIYRIKLPGPLKL 1133

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYR +YGIRKPTILGVRE
Sbjct: 1134 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRHNYGIRKPTILGVRE 1193

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS
Sbjct: 1194 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 1253

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQVSMFEAKVASGNGEQVLSRD
Sbjct: 1254 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQVSMFEAKVASGNGEQVLSRD 1313

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIEN IASESNNG
Sbjct: 1314 VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENAIASESNNG 1373

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLE GFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1374 ALATILNQQFIIQLGLFTALPMIVENSLEHGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1433

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLIL VYASHSAVS+
Sbjct: 1434 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILIVYASHSAVSS 1493

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            +TFVYIAMTFTSWFLV SWLMAPFVFNPSGFDWLKTVYDFD+FMNWIWYRGSIFAKAEQS
Sbjct: 1494 DTFVYIAMTFTSWFLVGSWLMAPFVFNPSGFDWLKTVYDFDDFMNWIWYRGSIFAKAEQS 1553

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLE+ILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWI V
Sbjct: 1554 WERWWYEEQDHLKTTGFWGKVLEIILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWIYV 1613

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYARD+YAAKEHIYYRLVQFLIIILA+V+IVALLEFTAF+F DIFTSLLA
Sbjct: 1614 FVALATYVVVAYARDKYAAKEHIYYRLVQFLIIILAVVIIVALLEFTAFRFIDIFTSLLA 1673

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWGLLLIAQVLRPFL STILW++VI+VARFYDILFGVIVMIPVAVLSWLPGFQSMQ
Sbjct: 1674 FLPTGWGLLLIAQVLRPFLQSTILWEVVISVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1733

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1734 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1762

BLAST of Cucsat.G17995 vs. ExPASy TrEMBL
Match: A0A6J1JEY4 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111483813 PE=3 SV=1)

HSP 1 Score: 2627 bits (6810), Expect = 0.0
Identity = 1327/1409 (94.18%), Postives = 1366/1409 (96.95%), Query Frame = 0

Query: 1    MQYSLVSRETLGLGVRMIMKSIVAAAWTILFVVFYVRIWSQRSRDRVWSAQANKDVGNFL 60
            MQYSLVSRETLG  VRMIMKSIVAAAWT+LF+VFY RIWSQRS+DR WSAQA+KD+GNFL
Sbjct: 358  MQYSLVSRETLGPAVRMIMKSIVAAAWTVLFIVFYWRIWSQRSQDRAWSAQADKDIGNFL 417

Query: 61   IAAGVFIAPEVLALALFILPWIRNFMEETNWKVFYMLSWWFQSRTFVGRGLREGLVDNIK 120
            IAAGVFIAPEVLALALFILPWIRNFMEETNW++FYMLSWWFQSRTFVGRGLREGLVDNIK
Sbjct: 418  IAAGVFIAPEVLALALFILPWIRNFMEETNWRIFYMLSWWFQSRTFVGRGLREGLVDNIK 477

Query: 121  YSLFWILVLATKFSFSYFLQIKPMMAPTRALLNLGDVPYEWHQFFRGSNRFAVVLLWLPV 180
            YS+FWILVLA+KFSFSYFLQIKPMM PT+ALL LGDV YEWHQFFRGSNRFAV+LLWLPV
Sbjct: 478  YSMFWILVLASKFSFSYFLQIKPMMGPTKALLKLGDVSYEWHQFFRGSNRFAVLLLWLPV 537

Query: 181  VLIYLMDLQIWYSIYSSFVGAAVGLLDHLGEIRNMPQLRLRFQFFASAIQFNLMPEEQLL 240
            +LIYLMDLQIWYSIYSSFVGAAVGL DHLGEIRN+PQLRLRFQFFASAIQFNLMP+EQLL
Sbjct: 538  LLIYLMDLQIWYSIYSSFVGAAVGLFDHLGEIRNIPQLRLRFQFFASAIQFNLMPQEQLL 597

Query: 241  NARGTLRSKFKDAIHRLKLRYGLGHSYKKLESNQVEATKFAIIWNEIITIFREEDIISDR 300
            NARGTL+SKFKDAIHRLKLRYGLGHSY KLESNQVEATKFAIIWNEIITIFREEDIISDR
Sbjct: 598  NARGTLKSKFKDAIHRLKLRYGLGHSYNKLESNQVEATKFAIIWNEIITIFREEDIISDR 657

Query: 301  EVELLELPQNSWSIKVIRWPCFLLCNELLLALSQAKELIDAPDKWLWHKICKNEYRRCAV 360
            EVELLELP NSWSIKV+RWPCFLLCNELLL+LSQAKEL DA DKWLWHKICKNEYRRCAV
Sbjct: 658  EVELLELPLNSWSIKVVRWPCFLLCNELLLSLSQAKELTDATDKWLWHKICKNEYRRCAV 717

Query: 361  IEAYESIKHLLLQILKHNSEEKSIMTVLFQEIDHSIAIEKFTKTFNMNALPDLHAKLIIL 420
            IEAYESIKHLLLQILKHN+EEKSIMTVLFQEIDHSI IEKFTKTFNMNALPDLH KLI L
Sbjct: 718  IEAYESIKHLLLQILKHNTEEKSIMTVLFQEIDHSIEIEKFTKTFNMNALPDLHTKLIKL 777

Query: 421  AELLNKPKKDTNQVVNTLQALYEIATRDFFKEKRTGAQLINDGLALRNSTSTTGLLFENA 480
            AELL KPKKDT  VVNTLQA+YEI TRDFFKEKR+G QLI DGLALRNSTSTTGLLFENA
Sbjct: 778  AELLTKPKKDTIGVVNTLQAVYEITTRDFFKEKRSGDQLIIDGLALRNSTSTTGLLFENA 837

Query: 481  VQFPDVTNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 540
            V+FPDV NESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK
Sbjct: 838  VKFPDVGNESFYRQVRRLHTILTSRDSMHNIPINLEARRRLAFFSNSLFMNIPHAPQVEK 897

Query: 541  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMHREGMVIDR 600
            MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERM REGM  D 
Sbjct: 898  MMAFSVLTPYYSEEVLYSKEQLRTENEDGISILYYLQTIYVDEWKNFLERMRREGMGKDS 957

Query: 601  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELDSMR 660
            EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQEL SM 
Sbjct: 958  EIWTTKLRDLRLWASFRGQTLTRTVRGMMYYYRALKMLAYLDSASEMDIREGSQELGSMG 1017

Query: 661  REGSIDGIASDRSTPSRSLSRMGSSVSLLFKGHEYGTALMKYTYVVACQIYGTQKAKKDP 720
            +EGSID +ASDRS  +RSLSR GSSVS LFKGHEYGTALMKYTYVVACQIYG+QKAKKDP
Sbjct: 1018 QEGSIDSVASDRSLQTRSLSRTGSSVSFLFKGHEYGTALMKYTYVVACQIYGSQKAKKDP 1077

Query: 721  HAEEILYLMKTNEALRVAYVDEVSTGREEKEYYSVLVKYDHVLEKEVEIYRIKLPGPLKL 780
            HAEEILYLMK+NEALRVAYVDEV TGREEKEYYSVLVKYDH LEKEVEIYRIKLPGPLKL
Sbjct: 1078 HAEEILYLMKSNEALRVAYVDEVCTGREEKEYYSVLVKYDHQLEKEVEIYRIKLPGPLKL 1137

Query: 781  GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEYRRSYGIRKPTILGVRE 840
            GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEE++RSYGIRKPTILGVRE
Sbjct: 1138 GEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEFKRSYGIRKPTILGVRE 1197

Query: 841  HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDVFDRFWFLTRGGISKAS 900
            HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPD+FDRFWFLTRGGISKAS
Sbjct: 1198 HIFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKIRMHYGHPDMFDRFWFLTRGGISKAS 1257

Query: 901  RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQVLSRD 960
            RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVG NQVSMFEAKVASGNGEQVLSRD
Sbjct: 1258 RVINISEDIFAGFNCTLRGGNVTHHEYIQVGKGRDVGFNQVSMFEAKVASGNGEQVLSRD 1317

Query: 961  VYRLGHRLDFFRMLSFFYTTVGFFFNTMMVTLTVYAFLWGRLYLALSGIENTIASESNNG 1020
            VYRLGHRLDFFRMLSFFY+TVGFFFNTMMVTLTVY FLWGRLYLALSGIEN IA ESNNG
Sbjct: 1318 VYRLGHRLDFFRMLSFFYSTVGFFFNTMMVTLTVYVFLWGRLYLALSGIENEIARESNNG 1377

Query: 1021 ALATILNQQFIIQLGLFTALPMIVENSLEQGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1080
            ALATILNQQFIIQLGLFTALPMIVENSLE GFLQSIWDFLTMQLQLSSIFYTFSMGTRAH
Sbjct: 1378 ALATILNQQFIIQLGLFTALPMIVENSLEHGFLQSIWDFLTMQLQLSSIFYTFSMGTRAH 1437

Query: 1081 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILTVYASHSAVST 1140
            YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLIL VYASH+AVS+
Sbjct: 1438 YFGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGLILIVYASHNAVSS 1497

Query: 1141 NTFVYIAMTFTSWFLVISWLMAPFVFNPSGFDWLKTVYDFDEFMNWIWYRGSIFAKAEQS 1200
            NTFVYIAMTFTSWFLV+SWLMAPFVFNPSGFDWLKTVYDFD+FMNWIWYRGSIFAKAEQS
Sbjct: 1498 NTFVYIAMTFTSWFLVLSWLMAPFVFNPSGFDWLKTVYDFDDFMNWIWYRGSIFAKAEQS 1557

Query: 1201 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSTSIAVYLLSWICV 1260
            WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGS SIAVYLLSWICV
Sbjct: 1558 WERWWYEEQDHLKTTGFWGKVLEVILDLRFFFFQYGVVYQLGISAGSKSIAVYLLSWICV 1617

Query: 1261 FVALATYVVVAYARDRYAAKEHIYYRLVQFLIIILAIVVIVALLEFTAFKFRDIFTSLLA 1320
            FVALATYVVVAYAR++YAAK+HIYYRLVQFLIIILAIVVIVALLEFTAF+FRDIFTSLLA
Sbjct: 1618 FVALATYVVVAYARNKYAAKDHIYYRLVQFLIIILAIVVIVALLEFTAFEFRDIFTSLLA 1677

Query: 1321 FLPTGWGLLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMIPVAVLSWLPGFQSMQ 1380
            FLPTGWG LLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVM+PVAVLSWLPGFQSMQ
Sbjct: 1678 FLPTGWGFLLIAQVLRPFLHSTILWDIVIAVARFYDILFGVIVMVPVAVLSWLPGFQSMQ 1737

Query: 1381 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1409
            TRILFNEAFSRGLRIFQIVTGKKSKVDQD
Sbjct: 1738 TRILFNEAFSRGLRIFQIVTGKKSKVDQD 1766