Cucsat.G17968 (gene) Cucumber (B10) v3

Overview
NameCucsat.G17968
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionPhosphoglycerate kinase
Locationctg3345: 2382860 .. 2386152 (+)
RNA-Seq ExpressionCucsat.G17968
SyntenyCucsat.G17968
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACTATGATGAAATTTACAAATGTACAACAATGGAGTGGACAAAAGCTTGAAGAAATCAACCTAGTTCATCACACAATCTCGAAAGCATGGATCGTCAACCTCATCTCAACGATAAGAGATATGTGTGATCAAAGCCACAAATTGATTATTGCCCATTCGTTCTCATTGTTCTTCTCGTCCATTCAATCTCTCTCCCCCTCCACAAATCATCTTCATCCTTCCATTTCTTCCACATAAAAATACTTCCCCAATTCGCCAAATCGCTTCCTTCTCTGTTTTTCACTCACCCACAAAAACCCACTTCACTCTTCTCCTCTCTCATCATCATCAATGGCGTCTGCAGCTTCTCGTGCAACTTTCCATCTCCTTCCCACCACATCCTCTTCCTCCTCTTCAACCCGCTCTGCCCTTCTTCAATTTTCCCCATCTACCACTTCCTCTTCCTCAATTCGCCTCCGTAGCGCCGCCACTCCTCGCCTTGGCTTTGCCGCTGCAGACCCACTTTTCTCCCTCCATGTAGCTTCCAAGATTCGGTCGTTTGGGGGGAAGGCTTCGAGGGGTGTGGTGTCCATGGCTAAGAAGAGCGTTGGGGATTTGTCTGCCGCTGACTTGAAGGGGAAGAAGGTGTTCGTGAGAGCTGATCTGAATGTGCCTTTGGATGATAATCAGAATATTACTGACGACACTAGGATCAGAGCTGCTATTCCTACAATCAAGCATTTGACGGAGAAGGGAGCTAAAGTTATTCTATCCAGTCATTTGGTGAGTGTACCTTTGGGACTTCTTGTTTACTAGTTAGACTTGTTCTGTTGTTTCTGGATGACAGTAGCTATAAAGTCGTTTGAAGTTATTGATGGGTTGATGGTTTTGGTGATTTTGTATGTGACAGTCTCTTGGTTAATGGAATTTGTAGAGAAAAGGTTAAGGGATGTTAGCTGTGATGGTACATTGCATGTAATGACAATTGGGTTTTTGGTTTTTTGATTAGCCATCCATGGAAACTTGTGATCAGGGATCAGATTAGAGTGTTGACAGTATACTGTTTATGTTGCACAAGCTTGTGAATATGTTGTTAAACAATGGAGTTTTCAGGGACGACCAAAAGGTGTGACTCCAAAGTACAGCTTGGCTCCTCTTGTGCCTAGGCTTTCTGAACTCCTTAGCATTCAGGTACCTTTTCCTTCATATGATCTGAAAGAAGGGTTTGTTTTACTGTTCTTTGATTCCTTCCTTTCCCGTATGTTGTTCATTTAACGCCTCAGAGTTACACTAAACATTTCTAGATGTCATGTCCAAGCAGGTTGTGAAGGCTGATGACTGCATTGGTCCGGAGGTCGAAAAGTTGGTTGCTTCCCTGCCCGACGGTGGAGTTCTTCTCCTTGAAAATGTGAGATTCTACAAGGAAGAAGAGAAAAACGAACCCGAGTTTGCAAAGAAGCTTGCTTCTCTAGCCGATCTTTTTGTGAATGATGCATTTGGGACTGCACATAGAGCTCACGCTTCAACCGAGGGAGTCACCAAGTTTTTAAAGCCATCCGTAGCTGGTTTCCTCTTGCAGAAGGTTGGATCTTTTTCTCCTTGTAGGATTCACTGGTTACTTGCACAAATACCATATGTTACTAATTATATACTATTTTTCTTTTTCACTGATCTCGTATGTAACTGGTAACCGGCCTCAATTTGTTCACAATCTCCAAACTTCAATACATTATGGTTGGCTCAATGTTTATCATGTTATTTTATTGCTGCTATGTCTAATGATTTTTAACCCTGCGGGTAATCACTTTAGGAACTCGACTATCTAGTGGGGGCCGTGTCAAGCCCGAAGAGGCCATTTGCTGCTATTGTTGGTGGTTCAAAGGTTTCTTCAAAGATTGGAGTGATTGAGTCACTTCTAGAAAAATGTGACATCCTACTCCTCGGTGGTGGAATGATCTTTACATTTTACAAGGCACAGGGACTTTCCGTTGGTTCATCTTTGGTGGAAGAGGATAAGCTAGAACTAGCGACCTCCCTCCTTGAGAAGGCCAAGGCTAAAGGAGTATCCCTTTTATTACCAACCGATGTCATCATTGCAGACAAGTTTGCTCCTGATGCAAACAGCAAGGTTTGTTTGTGAATCTTGGTTCTTAGATCCATTTCTCAAACCAATGCACAGAAACACAAGCTTCTGAAATTTTATTGATTGATTCAACTAGTCTTGGTTTTCCAGTCTCTTGGAAACTTCAAAACTATGGATAGGGAAATAAAGAACATGTTGTTTTAAATTGCAGATTGTCCCAGCATCTGCCATCCCTGATGGATGGATGGGGCTGGACATTGGACCAGATTCCGTAAAGACATTCAATGATGCTTTGGACACTACCCAAACTATCATCTGGAATGGACCAATGGGAGTGTTCGAATTCGACAAGTTTGCAGTGGGCACAGAGGTAAGAAAGAACTTCCCCATGGTGATAAAAATGCATTTTAATACTTCACATTGATGTCATTTGCATACTTATATAGCCGATTTGTATTTGTATGTGAGTCATAAATATTGATTTGAAGTTAAACCAAGGAGTAAGGATAGCGTTTCTCTTTTTAACCTTCATGTTATTGCAACACACCTCCCCTATTATTGGAGGTGGGGGATGGCATACCGATCTCAACATGTTTGTTATAGTGCTGGAACTCTGTGAGATCTTGAATATTCATGGTTCTCTGAGGTGCCTGATTTGTTTGGTTCATCAAAGTATGACATGATAGAGACCTATAAACCAATGCAGTTCAACATTTGCACACTATGTATAGCAGATGTGTTTATGTCGCCAGTACCAATCTGAAATATGCACTTGGTTGCAGGCCATTGCTAAAAAACTCGCAGAACTCAGCGGAAAGGGGGTGACGACCATCATCGGAGGTGGAGACTCGGTTGCAGCCGTGGAGAAGGTGGGAGTTGCAAACGTCATGAGCCACATTTCAACCGGTGGGGGTGCTAGTTTGGAGTTGTTGGAAGGCAAAGAGCTACCGGGTGTCCTCGCTCTTGATGAGGCCGTACCTGTCGCCGTGTAGGTGATGGATTATTATTCAATTTGATTTTTTTTTTCTCCCATTGCAGCTGAATGCTTAAATTGCAATGTGTAATAGAGAAATATATATATTGTCGTAGAAGAAGATGAGTTTTTGTTGTAAAAGTGGTAACTTCAACTGTTGTTCCCTTTCTGTCTCTGCATTTGACTTTTCCTTGCTTGCTGGGAACAGATTGTAGGGATAACAACTGCTTTAAGAGTTGTAATAAATTTGTGCATGGATGTCCATTTTCCTCTCAATTATTTTCATTTTTTCTCGTAGATAAAATTCAAAAATAATAATTAGGTGCAACCTTATAT

Coding sequence (CDS)

ATGGCGTCTGCAGCTTCTCGTGCAACTTTCCATCTCCTTCCCACCACATCCTCTTCCTCCTCTTCAACCCGCTCTGCCCTTCTTCAATTTTCCCCATCTACCACTTCCTCTTCCTCAATTCGCCTCCGTAGCGCCGCCACTCCTCGCCTTGGCTTTGCCGCTGCAGACCCACTTTTCTCCCTCCATGTAGCTTCCAAGATTCGGTCGTTTGGGGGGAAGGCTTCGAGGGGTGTGGTGTCCATGGCTAAGAAGAGCGTTGGGGATTTGTCTGCCGCTGACTTGAAGGGGAAGAAGGTGTTCGTGAGAGCTGATCTGAATGTGCCTTTGGATGATAATCAGAATATTACTGACGACACTAGGATCAGAGCTGCTATTCCTACAATCAAGCATTTGACGGAGAAGGGAGCTAAAGTTATTCTATCCAGTCATTTGGGACGACCAAAAGGTGTGACTCCAAAGTACAGCTTGGCTCCTCTTGTGCCTAGGCTTTCTGAACTCCTTAGCATTCAGGTTGTGAAGGCTGATGACTGCATTGGTCCGGAGGTCGAAAAGTTGGTTGCTTCCCTGCCCGACGGTGGAGTTCTTCTCCTTGAAAATGTGAGATTCTACAAGGAAGAAGAGAAAAACGAACCCGAGTTTGCAAAGAAGCTTGCTTCTCTAGCCGATCTTTTTGTGAATGATGCATTTGGGACTGCACATAGAGCTCACGCTTCAACCGAGGGAGTCACCAAGTTTTTAAAGCCATCCGTAGCTGGTTTCCTCTTGCAGAAGGAACTCGACTATCTAGTGGGGGCCGTGTCAAGCCCGAAGAGGCCATTTGCTGCTATTGTTGGTGGTTCAAAGGTTTCTTCAAAGATTGGAGTGATTGAGTCACTTCTAGAAAAATGTGACATCCTACTCCTCGGTGGTGGAATGATCTTTACATTTTACAAGGCACAGGGACTTTCCGTTGGTTCATCTTTGGTGGAAGAGGATAAGCTAGAACTAGCGACCTCCCTCCTTGAGAAGGCCAAGGCTAAAGGAGTATCCCTTTTATTACCAACCGATGTCATCATTGCAGACAAGTTTGCTCCTGATGCAAACAGCAAGATTGTCCCAGCATCTGCCATCCCTGATGGATGGATGGGGCTGGACATTGGACCAGATTCCGTAAAGACATTCAATGATGCTTTGGACACTACCCAAACTATCATCTGGAATGGACCAATGGGAGTGTTCGAATTCGACAAGTTTGCAGTGGGCACAGAGGCCATTGCTAAAAAACTCGCAGAACTCAGCGGAAAGGGGGTGACGACCATCATCGGAGGTGGAGACTCGGTTGCAGCCGTGGAGAAGGTGGGAGTTGCAAACGTCATGAGCCACATTTCAACCGGTGGGGGTGCTAGTTTGGAGTTGTTGGAAGGCAAAGAGCTACCGGGTGTCCTCGCTCTTGATGAGGCCGTACCTGTCGCCGTGTAG

Protein sequence

MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFSLHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEAIAKKLAELSGKGVTTIIGGGDSVAAVEKVGVANVMSHISTGGGASLELLEGKELPGVLALDEAVPVAV
Homology
BLAST of Cucsat.G17968 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.3e-187
Identity = 355/420 (84.52%), Postives = 375/420 (89.29%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGF--AAADPL 60
           MASA +  T   +P TSSS+++   A          SS+  L      RLGF  AAAD L
Sbjct: 1   MASATASHTLCGIPATSSSTTNKAIA---------PSSARFLAKTPLRRLGFAGAAADSL 60

Query: 61  FSLHVASKIRSF--GGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNIT 120
           F+ HVA+K+RS     K  RGV SMAKKSVGDL+AA+LKGKKVFVRADLNVPLDDNQNIT
Sbjct: 61  FTNHVATKLRSLKSSSKPIRGVASMAKKSVGDLTAAELKGKKVFVRADLNVPLDDNQNIT 120

Query: 121 DDTRIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADD 180
           DDTRIRAA+PTIKHL   GAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL IQVVK +D
Sbjct: 121 DDTRIRAAVPTIKHLMANGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVED 180

Query: 181 CIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAH 240
           CIGPEVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAH
Sbjct: 181 CIGPEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAH 240

Query: 241 ASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKC 300
           ASTEGVTKFLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKC
Sbjct: 241 ASTEGVTKFLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKC 300

Query: 301 DILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKF 360
           DILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLP+DV+IADKF
Sbjct: 301 DILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPSDVVIADKF 360

Query: 361 APDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 417
           APDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTT+T+IWNGPMGVFEFDKFAVGTE
Sbjct: 361 APDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTE 411

BLAST of Cucsat.G17968 vs. ExPASy Swiss-Prot
Match: P50318 (Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g56190 PE=1 SV=3)

HSP 1 Score: 650.6 bits (1677), Expect = 1.3e-185
Identity = 346/421 (82.19%), Postives = 376/421 (89.31%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAA-ADPLF 60
           MAS A+ A   ++ +T   ++ TRS+   F    ++S S R       RLGF+A  D  F
Sbjct: 1   MASTAATAALSIIKST-GGAAVTRSSRASFGHIPSTSVSAR-------RLGFSAVVDSRF 60

Query: 61  SLHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDT 120
           S+HVASK+ S  GK +RGV++MAKKSVGDL++ DLKGKKVFVRADLNVPLDDNQNITDDT
Sbjct: 61  SVHVASKVHSVRGKGARGVITMAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDT 120

Query: 121 RIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIG 180
           RIRAAIPTIK L E GAKVILS+HLGRPKGVTPK+SLAPLVPRLSELL I+VVKADDCIG
Sbjct: 121 RIRAAIPTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIG 180

Query: 181 PEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST 240
           PEVE LVASLP+GGVLLLENVRFYKEEEKNEP+FAKKLASLADL+VNDAFGTAHRAHAST
Sbjct: 181 PEVETLVASLPEGGVLLLENVRFYKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHAST 240

Query: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300
           EGVTKFLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEKCDIL
Sbjct: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300

Query: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD 360
           LLGGGMIFTFYKAQGLSVGSSLVEEDKLELAT+LL KAKA+GVSLLLPTDV+IADKFAPD
Sbjct: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPD 360

Query: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTEVRK 420
           ANSKIVPASAIPDGWMGLDIGPDSVKTFN+ALDTTQT+IWNGPMGVFEF+KFA GTE   
Sbjct: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVA 413

BLAST of Cucsat.G17968 vs. ExPASy Swiss-Prot
Match: Q9LD57 (Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1 PE=1 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 2.4e-184
Identity = 350/426 (82.16%), Postives = 376/426 (88.26%), Query Frame = 0

Query: 1   MASAASRATFHLLPTT----SSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAA- 60
           MASAA+ + F LL +T    SS+ +  R++LL   PST+ S        A P LGF+A  
Sbjct: 1   MASAAASSAFSLLKSTGAVASSAGTRARASLLPI-PSTSVS--------ARP-LGFSATL 60

Query: 61  -DPLFSLHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQN 120
               FSLHVASK+ S  GK SRGVVSMAKKSVGDL++ADLKGKKVFVRADLNVPLDDNQ 
Sbjct: 61  DSRRFSLHVASKVESVRGKGSRGVVSMAKKSVGDLTSADLKGKKVFVRADLNVPLDDNQT 120

Query: 121 ITDDTRIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKA 180
           ITDDTRIRAAIPTIK+L E GAKVILS+HLGRPKGVTPK+SLAPLVPRLSELL I+V KA
Sbjct: 121 ITDDTRIRAAIPTIKYLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKA 180

Query: 181 DDCIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHR 240
           DDCIGPEVE LVASLP+GGVLLLENVRFYKEEEKN+PEFAKKLASLADL+VNDAFGTAHR
Sbjct: 181 DDCIGPEVESLVASLPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHR 240

Query: 241 AHASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLE 300
           AHASTEGVTKFLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLE
Sbjct: 241 AHASTEGVTKFLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLE 300

Query: 301 KCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIAD 360
           KCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELAT LL KAKAKGVSLLLPTDV++AD
Sbjct: 301 KCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVAD 360

Query: 361 KFAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVG 420
           KFAPDANSKIVPAS I DGWMGLDIGPDS+KTFN+ALDTTQT+IWNGPMGVFE +KFA G
Sbjct: 361 KFAPDANSKIVPASGIEDGWMGLDIGPDSIKTFNEALDTTQTVIWNGPMGVFEMEKFAAG 416

BLAST of Cucsat.G17968 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 630.2 bits (1624), Expect = 1.8e-179
Identity = 320/361 (88.64%), Postives = 343/361 (95.01%), Query Frame = 0

Query: 59  FSLHVASKIRSFGGKAS---RGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNI 118
           FSLHV SKI S+  +++   RGV SMAKKSVGDL++ADLKGKKVFVRADLNVPLDD+QNI
Sbjct: 4   FSLHVLSKINSYKSQSTKPIRGVASMAKKSVGDLTSADLKGKKVFVRADLNVPLDDSQNI 63

Query: 119 TDDTRIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKAD 178
           TDDTRIRAAIPTIKHL   GAKVILSSHLGRPKGVTPK+SLAPLVPRLSELL +QVVKAD
Sbjct: 64  TDDTRIRAAIPTIKHLINNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKAD 123

Query: 179 DCIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRA 238
           DCIGP+VEKLVA LP+GGVLLLENVRFYKEEEKN+PEFAKKLASLADL+VNDAFGTAHRA
Sbjct: 124 DCIGPDVEKLVAELPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRA 183

Query: 239 HASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEK 298
           HASTEGVTKFLKPSVAGFLLQKELDYLVGAVS+PKRPFAAIVGGSKVSSKIGVIESLLEK
Sbjct: 184 HASTEGVTKFLKPSVAGFLLQKELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEK 243

Query: 299 CDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADK 358
           CDILLLGGGMIFTFYKAQG+SVGSSLVEEDKL+LATSLL KAK KGVSLLLPTDV+IADK
Sbjct: 244 CDILLLGGGMIFTFYKAQGMSVGSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADK 303

Query: 359 FAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGT 417
           FA DA+SKIVPAS IPDGWMGLDIGPDS+KTF++ALDTTQT+IWNGPMGVFEF+KFA GT
Sbjct: 304 FAADADSKIVPASGIPDGWMGLDIGPDSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGT 363

BLAST of Cucsat.G17968 vs. ExPASy Swiss-Prot
Match: P12782 (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.7e-177
Identity = 324/369 (87.80%), Postives = 344/369 (93.22%), Query Frame = 0

Query: 50  LGFAA-ADPLFSLHVASKIR-SFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNV 109
           L FAA ADP  ++HVAS+ R +   + +R V +MAKKSVGDL+AADL+GK+V VRADLNV
Sbjct: 40  LAFAAGADPRLAVHVASRCRAASAARGTRAVATMAKKSVGDLTAADLEGKRVLVRADLNV 99

Query: 110 PLDDNQNITDDTRIRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL 169
           PLDDNQNITDDTRIRAAIPTIK+L   GAKVIL+SHLGRPKGVTPK+SLAPLVPRLSELL
Sbjct: 100 PLDDNQNITDDTRIRAAIPTIKYLLSNGAKVILTSHLGRPKGVTPKFSLAPLVPRLSELL 159

Query: 170 SIQVVKADDCIGPEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVND 229
            I+V KA+D IGPEVEKLVA L +G VLLLENVRFYKEEEKN+PEFAKKLASLADLFVND
Sbjct: 160 GIEVKKAEDVIGPEVEKLVADLANGAVLLLENVRFYKEEEKNDPEFAKKLASLADLFVND 219

Query: 230 AFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIG 289
           AFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL GAVS+PKRPFAAIVGGSKVSSKIG
Sbjct: 220 AFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLDGAVSNPKRPFAAIVGGSKVSSKIG 279

Query: 290 VIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLP 349
           VIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLL KAKAKGVSLLLP
Sbjct: 280 VIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLP 339

Query: 350 TDVIIADKFAPDANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFE 409
           +DVIIADKFAPDANS+ VPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFE
Sbjct: 340 SDVIIADKFAPDANSQTVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFE 399

Query: 410 FDKFAVGTE 417
           FDKFAVGTE
Sbjct: 400 FDKFAVGTE 408

BLAST of Cucsat.G17968 vs. NCBI nr
Match: XP_004139904.1 (phosphoglycerate kinase, chloroplastic [Cucumis sativus] >KGN46797.1 hypothetical protein Csa_020712 [Cucumis sativus])

HSP 1 Score: 787 bits (2033), Expect = 4.02e-285
Identity = 416/416 (100.00%), Postives = 416/416 (100.00%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. NCBI nr
Match: XP_008456300.1 (PREDICTED: phosphoglycerate kinase, chloroplastic [Cucumis melo] >TYK08584.1 phosphoglycerate kinase [Cucumis melo var. makuwa])

HSP 1 Score: 780 bits (2015), Expect = 2.21e-282
Identity = 411/416 (98.80%), Postives = 414/416 (99.52%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL  QVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. NCBI nr
Match: KAA0054686.1 (phosphoglycerate kinase [Cucumis melo var. makuwa])

HSP 1 Score: 776 bits (2003), Expect = 1.55e-280
Identity = 411/417 (98.56%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQ-VVKADDCIG 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL  Q VVKADDCIG
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQQVVKADDCIG 180

Query: 181 PEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST 240
           PEVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST
Sbjct: 181 PEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST 240

Query: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300
           EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL
Sbjct: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300

Query: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD 360
           LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD
Sbjct: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD 360

Query: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 417

BLAST of Cucsat.G17968 vs. NCBI nr
Match: XP_038901653.1 (phosphoglycerate kinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 774 bits (1998), Expect = 8.60e-280
Identity = 407/416 (97.84%), Postives = 413/416 (99.28%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRS+AT RLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSSATQRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL IQVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADL+VNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLL KAKAKGVSLLLPTDV+IADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. NCBI nr
Match: KAG6570594.1 (hypothetical protein SDJN03_29509, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 758 bits (1956), Expect = 2.14e-273
Identity = 399/416 (95.91%), Postives = 408/416 (98.08%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAAS ATFHLLPTTSSS+SSTRSALLQFSP+ TSSSSIRLRSA   RLGFAA+DPLFS
Sbjct: 1   MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFAASDPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSF GKASRGVVSMAKKSVGDL+A+DLKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPK+SLAPLVPRLSELL IQVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLL KAKAKGVSLLLPTDV+IADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. ExPASy TrEMBL
Match: A0A0A0KEF3 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G136020 PE=3 SV=1)

HSP 1 Score: 787 bits (2033), Expect = 1.94e-285
Identity = 416/416 (100.00%), Postives = 416/416 (100.00%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. ExPASy TrEMBL
Match: A0A5D3CBI0 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3734G00020 PE=3 SV=1)

HSP 1 Score: 780 bits (2015), Expect = 1.07e-282
Identity = 411/416 (98.80%), Postives = 414/416 (99.52%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL  QVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. ExPASy TrEMBL
Match: A0A1S3C465 (Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103496286 PE=3 SV=1)

HSP 1 Score: 780 bits (2015), Expect = 1.07e-282
Identity = 411/416 (98.80%), Postives = 414/416 (99.52%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL  QVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

BLAST of Cucsat.G17968 vs. ExPASy TrEMBL
Match: A0A5A7UK91 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G005100 PE=3 SV=1)

HSP 1 Score: 776 bits (2003), Expect = 7.48e-281
Identity = 411/417 (98.56%), Postives = 414/417 (99.28%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAASRATFHLLPTTSSS+SSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS
Sbjct: 1   MASAASRATFHLLPTTSSSTSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSFGGKASRGVVSMAKKSVGDL+AADLKGKKVFVRADLNVPLDDNQNITDDTR
Sbjct: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLTAADLKGKKVFVRADLNVPLDDNQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQ-VVKADDCIG 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELL  Q VVKADDCIG
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGFQQVVKADDCIG 180

Query: 181 PEVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST 240
           PEVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST
Sbjct: 181 PEVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHAST 240

Query: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300
           EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL
Sbjct: 241 EGVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDIL 300

Query: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD 360
           LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD
Sbjct: 301 LLGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPD 360

Query: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 ANSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 417

BLAST of Cucsat.G17968 vs. ExPASy TrEMBL
Match: A0A6J1JFE9 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483926 PE=3 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 1.04e-273
Identity = 399/416 (95.91%), Postives = 408/416 (98.08%), Query Frame = 0

Query: 1   MASAASRATFHLLPTTSSSSSSTRSALLQFSPSTTSSSSIRLRSAATPRLGFAAADPLFS 60
           MASAAS ATFHLLPTTSSS+SSTRSALLQFSP+ TSSSSIRLRSA   RLGFAA+DPLFS
Sbjct: 1   MASAASPATFHLLPTTSSSTSSTRSALLQFSPTATSSSSIRLRSAPIQRLGFAASDPLFS 60

Query: 61  LHVASKIRSFGGKASRGVVSMAKKSVGDLSAADLKGKKVFVRADLNVPLDDNQNITDDTR 120
           LHVASKIRSF GKASRGVVSMAKKSVGDL+A+DLKGKKVFVRADLNVPLD+NQNITDDTR
Sbjct: 61  LHVASKIRSFRGKASRGVVSMAKKSVGDLTASDLKGKKVFVRADLNVPLDENQNITDDTR 120

Query: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLSIQVVKADDCIGP 180
           IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPK+SLAPLVPRLSELL IQVVKADDCIGP
Sbjct: 121 IRAAIPTIKHLTEKGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGIQVVKADDCIGP 180

Query: 181 EVEKLVASLPDGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240
           EVEKLVASLP+GGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE
Sbjct: 181 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPEFAKKLASLADLFVNDAFGTAHRAHASTE 240

Query: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300
           GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL
Sbjct: 241 GVTKFLKPSVAGFLLQKELDYLVGAVSSPKRPFAAIVGGSKVSSKIGVIESLLEKCDILL 300

Query: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLEKAKAKGVSLLLPTDVIIADKFAPDA 360
           LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLL KAKAKGVSLLLPTDV+IADKFAPDA
Sbjct: 301 LGGGMIFTFYKAQGLSVGSSLVEEDKLELATSLLAKAKAKGVSLLLPTDVVIADKFAPDA 360

Query: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416
           NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE
Sbjct: 361 NSKIVPASAIPDGWMGLDIGPDSVKTFNDALDTTQTIIWNGPMGVFEFDKFAVGTE 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q429611.3e-18784.52Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P503181.3e-18582.19Phosphoglycerate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9LD572.4e-18482.16Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1... [more]
P294091.8e-17988.64Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
P127823.7e-17787.80Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004139904.14.02e-285100.00phosphoglycerate kinase, chloroplastic [Cucumis sativus] >KGN46797.1 hypothetica... [more]
XP_008456300.12.21e-28298.80PREDICTED: phosphoglycerate kinase, chloroplastic [Cucumis melo] >TYK08584.1 pho... [more]
KAA0054686.11.55e-28098.56phosphoglycerate kinase [Cucumis melo var. makuwa][more]
XP_038901653.18.60e-28097.84phosphoglycerate kinase, chloroplastic [Benincasa hispida][more]
KAG6570594.12.14e-27395.91hypothetical protein SDJN03_29509, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A0A0KEF31.94e-285100.00Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_6G136020 PE=3 SV=1[more]
A0A5D3CBI01.07e-28298.80Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C4651.07e-28298.80Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103496286 PE=3 SV=1[more]
A0A5A7UK917.48e-28198.56Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1JFE91.04e-27395.91Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483926 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 251..273
score: 52.13
coord: 192..207
score: 70.19
coord: 439..450
score: 62.91
coord: 220..242
score: 68.85
coord: 462..479
score: 85.96
coord: 115..137
score: 55.61
coord: 274..293
score: 60.33
coord: 93..109
score: 63.35
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 91..476
e-value: 1.5E-158
score: 527.8
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 1..487
IPR001576Phosphoglycerate kinaseHAMAPMF_00145Phosphoglyc_kinasecoord: 88..486
score: 40.931084
IPR001576Phosphoglycerate kinaseCDDcd00318Phosphoglycerate_kinasecoord: 92..486
e-value: 0.0
score: 615.033
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 87..263
e-value: 4.2E-76
score: 256.6
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 266..486
e-value: 5.3E-87
score: 292.8
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 89..320
e-value: 4.9E-55
score: 184.5
NoneNo IPR availablePANTHERPTHR11406:SF29PHOSPHOGLYCERATE KINASEcoord: 1..487
IPR015911Phosphoglycerate kinase, conserved sitePROSITEPS00111PGLYCERATE_KINASEcoord: 98..108
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 88..486

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G17968.T1Cucsat.G17968.T1mRNA
Cucsat.G17968.T3Cucsat.G17968.T3mRNA
Cucsat.G17968.T5Cucsat.G17968.T5mRNA
Cucsat.G17968.T4Cucsat.G17968.T4mRNA
Cucsat.G17968.T2Cucsat.G17968.T2mRNA
Cucsat.G17968.T6Cucsat.G17968.T6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004618 phosphoglycerate kinase activity