Cucsat.G17751 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G17751
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 704B1
Location	ctg31: 194909 .. 195774 (-)
RNA-Seq Expression	Cucsat.G17751
Synteny	Cucsat.G17751

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTACTTCATTTTGCTCAACTTCGATCCTGATACACATTTATACATTGAGAGATCCTTTTCTTGTAGTGTGATTTTTATGCTTTCTTGCAAATGCACCTAGTCTTATCACTTTGTACTACTCAGTATTGATTGATATAGTATATTTCATTTTATTGGTATAATATACATTTCATTTGATTGGTCATTTAATAATAACAATAAAAGGCCACATCATTAATTAAATTAAATGTAGCAGTTGCAAACAGTCACATCATATATACATTTCGAATTACAATAAACATTTTAATTTTCAAAATTTCAAGTGGATATTCTCGCAATTAGCAAATTATTATTTGTAGTTAGATATTGATCAATTAGATTCCTACTTTATCAAAATCTTGTTGTGAGTTAACCTAAAATAATATATATATATATATATATATATATATATATTAGGATCTGATGTTTTAAATAATTTTAACCATCACATGTTTTATAGCTAGTAACAAAGAAAATGACAAAAGTACTAAAATAAAACAAAAATACTTCATTTTCTTAAATGGATTGAAATGGTACCATTTCCCCCTCATTACTTTTAAAAACTACAATATTCATCCAAATTAACCAAAAATAATTCATTACAAAGCATGATTAGACACACATACGAGTTAAGATTTTGGCTACACATTCTCAATACTAAGAAGCATGCATACGTATTTAGAGCAGTATAAACCGGGTAATTAACTAATCATAAATTTAAAGGAGTGGTAGAAGGTAGGTGAACGTGTGGTATTGGGTAAACAAGAGAAATGGTTAGGTTAAGCACACAAAAGAGATGTTAATTATAAGTAATAAAGCATATAAAGATTGTTGTTTGGAAATGCAATAGCTATTAATTCCAAGTGTTAATAATATATTATATAAAGAGGTGGCATAATAGAGTTTGTTTTGGAAGCTATTGGTAAAATTAAGAAATGGGAATTAGTTTATTGGAGTGGTGTAATAATAATAATAGAGCTGATTCAGAAGAAGAATGGTACATGAGATCAAGTAATTTAAGAAGAAGTGGAGTAGTAGCGTTGGTGTTGGTGATGATAGCAATAATAAGTGCATGTTGGATAATTATTGTTCATAGATGGAACCAAAGGAAGAAGATGAGGAGAGGTCCAAAAACATGGCCAATCGTAGGAGCAGCAATAGAACAGTTTGTAAATTACCACAGAATGCATGATTGGATTTTGGGTTACCTTAATCTCTACAGAACTGTTGTGGTTCCCATGCCTTTCACTACTTACACTTATATTGCTGACCCTGCCAATGTTCAACATATTCTCAAGACCAACTTTGCTAATTATCCAAAGGTACAAGTACAATTGCCTTACATTCATCTTGTTAATTTACCTTCTTTTTTTTTTTCTTTCTTTTTAAAGAAAAAGTATGAGTAGTAATTTAGTTAATATTATTGTATTAGGGTGATGTATATCATTCATACATGGAAGTGCTTCTTGGAGATGGGATCTTCAACGTAGATGGAGAGTTGTGGAGGAAACAAAGGAAAACTGCTAGCTTCGAATTTGCATCTAAGAATTTGAGGGATTTCAGTACCTTAGTTTTTAGAGACTATGGTCTCAAGCTATCAACTATTTTAACACGTGCCTCTTTACTCAACCAACAAATTGACATGCAGGTGACTTTGTTCATCAAACCCAACCTTTTATTTATTTGTTTTTATTACAGTAAATAAATATTATTCATTTTTAAACGTAGGAATTGTTGATGAGGATGACTTTAGATTCCATATGCAAGGTTGGCTTTGGTGTTGAAATTGGATCATTGGCTCCCAATTTGCCAGAAAATAAGTTTGCTAAGGCCTTTGATACAGCCAATATGATCGTTACCCTTCGCTTCATTGATCCTCTGTGGAAAATTAAAAGATTTCTGAATCTGGGATCTGAAGCTCTTCTTGATAAATCCATCAAAACCATTGATGATTTCACTTATTCTGTCATTGCCACAAGGAAAAAAGAGATCCAAGAAGCTCAAACTACACACACAGATCCCAATAAGGTCATTTACCGTAATACTCTCTACTTCATTGCCCCCTTCATTTTATTAACTTCATTTTTCGTCTATTTTAATATTTTAACTTTGGAAAATTTTGCAAAACTAAAAATTCTTCTTTCAGTTTCACCTTTCATAAATAGCATCCACTGAATTTTGTATAAAAAGTTTACCTTTTCGACTTATATCTTGACCATTATTGATTCTCTTGAATTTTTTGGGTGATCTTTTTTATCTATCCAAAATTAAGAATCACACACGGCCAAATATTACGATAAATTTGGAAAAATGGCATAATGTTTGAAAAAAATATTGACATGGATCTCAATTCATCCTCCACACAAAATTGTACTAAAAAAAATCCAAAACAATTTATTATTTTGAGCTTTTTTGGTATAATTGTCTTCACAGTGAACACCAACATTAGACTGTCTTCGTAAAATTAAAAAAGGATTTTGGATTAATTTAAGTATATAGAACAAAACTGACCAATAACAAAATAAGCGTGTAAGGGTTGAGTTAATTTTTTTTCATAAATTCAGATTTATCATGTTAGAATTTATTATCTCCTTTTACATCATTTATTACTTGTTCGTCATCTTCTCATTTTCCATCGGTGTCATCTTTTTTATAAAGACAATATTTTCTTCTTGTTTCTTTTTTACTTTATCAAGTATTGTGTCCTTATTTTTTTCTTTTTTTGTCACTACATGATTAGTACAAGATTGTGTATTAAGATCGTTTAGATTGAGTACAATATCGGTTAGAATGATTACAATATTATTTAGTGTAGGTACTGCAACCAATTAATTACTGATATTTTTGTTATTTCACGTTGTGAGCTTGTGTGTTTTTTTCATTTTTAAAATTGTTTTATACGTTGTAAATATTGATGCGTTTTGTTTTTTTTAAAAAAAAAAACTCATTAATTATGATAATTTTTTCTCAACTTTCAACTTCAAATAAATCTTTTAACTTTTAAAAATGTAGAAAATACTAAAAGAAAAATTAAATTTGTCTATTATTATTTTTCAGTTTTTAAAGAAAGATATTATTTTGTAATAAAAAAATTGAAGCTTATATAAGGTGGAATTCAATTAATATATGTTTTTGGTATGAGACGATCACAGATGAAACATGACATATTGTCGAGATTTATTGAGTTAGGGGAAGACCCAAACAACAAATTCACAGACAAAAGCTTAAGAGATATTGTTCTGAACTTCGTGATTGCCGGACGTGACACAACAGCCGCAACTCTTTCATGGAGTATTTATATGCTAATGACTCATTCAGATGTCTCCCAAAAACTTTACTCAGAGCTCAAAAGTTTTGAAGAAGAAAGAGCCAAAGAACACTCCATTTCATTGCTTCAATATCAACCAAATGATATTCAATCCTTTGACACAAGAGTTGTACAATTTTCACAACTTCTTAATTATGATTCTCTTGCTCGATTACATTACTTGCATGCTATCGTTACTGAGACACTTCGTCTTTACCCTGCAGTTCCCCAGGTACGAAATTATACATTACTACTCCTTAGATAAACCTATGTTAAACTCGGTGGCAAACTTGCAAATACCATAATCAAACCTTAATGCAAAAGATAATCTAATAGATTTTGTTAAATATGTAATTATTTAAAATGTTGTTATACCCTTGAGGTATTAATTAATTTTAAAAGTGTTATCCATGCATTGCAATTATCCGATAGGACCCAAAGGGAATTTTGGAGGATGATGTATTGCCAGATGGGACAAAGGTAAGAGCTGGAGGAATGGTGACATATGTTCCTTACTCGATGGGAAGAATGGAATACAATTGGGGTTATGATGCAACTTCTTTTAAACCTGAAAGATGGCTCAAACAAGGCATTTTCCAGAATGAATCTCCATTCAAGTTCACAGCTTTTCAGGTCACTTTCCTTTTGATCTTCACTTATATGTTATCACTGAAACTATAAGAAGAGAATCGATCTGTTTCATTTAACACTTCAAGTATTTTGTTTTAAAATAATAATGTTATTATTCAAAATAAATAACTTTTTTTTCTCTAGTCAATTAGTAGACATTTAAGTCGTTTAATTAATTAGAGATTTTGAAATGTTATTGTAGGCTGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAAATGAAAATGACTCTTGCCATTTTATGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATAGAGTCGACTACAGAATGATGACTATTCTCTCAATGGCTAATGGCTTGAAAGTTACTGTGGCTCCACGCTCTTAAAAATTTTATCTCTTTTTATTTACACTTTATCATCAACAATCAAAACCAAAAACATATGTAATAAAATCTCATTTCCTTTTGAAAAAAACAAACTTGTTGATAATATATATAATTCTCATTTCTTTGTTTTTAAATCTTGAGAAACGTTTCACATTTAAAATCAAAATGTCTAATCTGACCATTGGTTCATTGGTGTTGATCGGACACTCTCGTTAATACTGATAATAATACTAGAAGTATTTGGGTTCAAATGCTAAATC

Coding sequence (CDS)

ATGGGAATTAGTTTATTGGAGTGGTGTAATAATAATAATAGAGCTGATTCAGAAGAAGAATGGTACATGAGATCAAGTAATTTAAGAAGAAGTGGAGTAGTAGCGTTGGTGTTGGTGATGATAGCAATAATAAGTGCATGTTGGATAATTATTGTTCATAGATGGAACCAAAGGAAGAAGATGAGGAGAGGTCCAAAAACATGGCCAATCGTAGGAGCAGCAATAGAACAGTTTGTAAATTACCACAGAATGCATGATTGGATTTTGGGTTACCTTAATCTCTACAGAACTGTTGTGGTTCCCATGCCTTTCACTACTTACACTTATATTGCTGACCCTGCCAATGTTCAACATATTCTCAAGACCAACTTTGCTAATTATCCAAAGGGTGATGTATATCATTCATACATGGAAGTGCTTCTTGGAGATGGGATCTTCAACGTAGATGGAGAGTTGTGGAGGAAACAAAGGAAAACTGCTAGCTTCGAATTTGCATCTAAGAATTTGAGGGATTTCAGTACCTTAGTTTTTAGAGACTATGGTCTCAAGCTATCAACTATTTTAACACGTGCCTCTTTACTCAACCAACAAATTGACATGCAGGAATTGTTGATGAGGATGACTTTAGATTCCATATGCAAGGTTGGCTTTGGTGTTGAAATTGGATCATTGGCTCCCAATTTGCCAGAAAATAAGTTTGCTAAGGCCTTTGATACAGCCAATATGATCGTTACCCTTCGCTTCATTGATCCTCTGTGGAAAATTAAAAGATTTCTGAATCTGGGATCTGAAGCTCTTCTTGATAAATCCATCAAAACCATTGATGATTTCACTTATTCTGTCATTGCCACAAGGAAAAAAGAGATCCAAGAAGCTCAAACTACACACACAGATCCCAATAAGATGAAACATGACATATTGTCGAGATTTATTGAGTTAGGGGAAGACCCAAACAACAAATTCACAGACAAAAGCTTAAGAGATATTGTTCTGAACTTCGTGATTGCCGGACGTGACACAACAGCCGCAACTCTTTCATGGAGTATTTATATGCTAATGACTCATTCAGATGTCTCCCAAAAACTTTACTCAGAGCTCAAAAGTTTTGAAGAAGAAAGAGCCAAAGAACACTCCATTTCATTGCTTCAATATCAACCAAATGATATTCAATCCTTTGACACAAGAGTTGTACAATTTTCACAACTTCTTAATTATGATTCTCTTGCTCGATTACATTACTTGCATGCTATCGTTACTGAGACACTTCGTCTTTACCCTGCAGTTCCCCAGGACCCAAAGGGAATTTTGGAGGATGATGTATTGCCAGATGGGACAAAGGTAAGAGCTGGAGGAATGGTGACATATGTTCCTTACTCGATGGGAAGAATGGAATACAATTGGGGTTATGATGCAACTTCTTTTAAACCTGAAAGATGGCTCAAACAAGGCATTTTCCAGAATGAATCTCCATTCAAGTTCACAGCTTTTCAGGCTGGACCAAGGATCTGCATGGGGAAAGACTCGGCATATCTACAAATGAAAATGACTCTTGCCATTTTATGCAGATTCTACAAATTTAGTTTGGTGAGTGGGCATAGAGTCGACTACAGAATGATGACTATTCTCTCAATGGCTAATGGCTTGAAAGTTACTGTGGCTCCACGCTCTTAA

Protein sequence

MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQELLMRMTLDSICKVGFGVEIGSLAPNLPENKFAKAFDTANMIVTLRFIDPLWKIKRFLNLGSEALLDKSIKTIDDFTYSVIATRKKEIQEAQTTHTDPNKMKHDILSRFIELGEDPNNKFTDKSLRDIVLNFVIAGRDTTAATLSWSIYMLMTHSDVSQKLYSELKSFEEERAKEHSISLLQYQPNDIQSFDTRVVQFSQLLNYDSLARLHYLHAIVTETLRLYPAVPQDPKGILEDDVLPDGTKVRAGGMVTYVPYSMGRMEYNWGYDATSFKPERWLKQGIFQNESPFKFTAFQAGPRICMGKDSAYLQMKMTLAILCRFYKFSLVSGHRVDYRMMTILSMANGLKVTVAPRS

Homology

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BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.3e-65
Identity = 122/176 (69.32%), Postives = 143/176 (81.25%), Query Frame = 0


Query: 39  VMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTV 98
           ++IA +   WI + HRW QR K   GPKTWP+VGAAIEQ  N+ RMHDW++ YL   RTV
Sbjct: 5   LVIACMVTSWIFL-HRWGQRNK--SGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTV 64

Query: 99  VVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRK 158
           VVPMPFTTYTYIADP NV+++LKTNF+NYPKG+ YHSYMEVLLGDGIFN DGELWRKQRK
Sbjct: 65  VVPMPFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRK 124

Query: 159 TASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQVTLFIKPNLLFIC 215
           TASFEFASKNLRDFST+VF++Y LKL TIL++AS   QQ+DMQ  L ++  L  IC
Sbjct: 125 TASFEFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQ-ELLMRMTLDSIC 176

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BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-32
Identity = 78/182 (42.86%), Postives = 111/182 (60.99%), Query Frame = 0


Query: 33  VVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYL 92
           ++ + + + A+  AC + I       +K    P   P+VG  +   +N+ R+HD+     
Sbjct: 5   ILTMFVTVSALALACSLWIASYLRNWRKKGVYP---PVVGTMLNHAINFERLHDYHTDQA 64

Query: 93  NLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGEL 152
             Y+T  V  P  +Y +  DP NV+HILKTNFANY KG   +  M+ LLGDGIFNVDG+ 
Sbjct: 65  QRYKTFRVVYPTCSYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGDGIFNVDGDK 124

Query: 153 WRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQIDMQVTLFIKPNLLF 212
           WR+QRK AS EFASK L+DFS+ VF +   KL+ IL +A+ LN  ++MQ  LF++ +L  
Sbjct: 125 WRQQRKLASSEFASKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQ-DLFMRSSLDS 182

Query: 213 IC 215
           IC
Sbjct: 185 IC 182

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BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.1e-26
Identity = 70/183 (38.25%), Postives = 97/183 (53.01%), Query Frame = 0


Query: 32  GVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGY 91
           G  A +++ + +    W + + R        +GP+ WP+VG+      N HRMHD+I   
Sbjct: 3   GSTAAIILTLIVTYIIWFVSLRR------SYKGPRVWPLVGSLPALITNAHRMHDFIADN 62

Query: 92  LNL----YRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLG 151
           L +    Y+T + P+PF        T   DP N++HILKT F NYPKG  + S    LLG
Sbjct: 63  LRMCGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLG 122

Query: 152 DGIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 205
           DGIFN DG+ WR QRKTA+ EF ++ LR   +  V R    +L  IL  A    + ID+Q
Sbjct: 123 DGIFNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQ 179

[Close]

BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 63/179 (35.20%), Postives = 94/179 (52.51%), Query Frame = 0


Query: 34  VALVLVMIAIISACWIIIVHRWNQR-KKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYL 93
           V+  ++++A+++A W+     W QR  +  +GP+ WP++G+         RMHDWI   L
Sbjct: 3   VSNTMLLVAVVAAYWL-----WFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENL 62

Query: 94  ----NLYRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLGD 153
                 Y+T +  +PF        T   DP N++H+LKT F NYPKG  + +     LG 
Sbjct: 63  RACGGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQ 122

Query: 154 GIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 202
           GIFN DG+ W  QRKTA+ EF ++ LR      V R   L+   IL  A    + +D+Q
Sbjct: 123 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQ 176

[Close]

BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.3e-23
Identity = 66/180 (36.67%), Postives = 95/180 (52.78%), Query Frame = 0


Query: 34  VALVLVMIAIISA--CWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGY 93
           ++  L++++ I+A   W+  + R        +GP+ WPI+G+      N  RMHDWI   
Sbjct: 3   ISTALMILSAITAYFLWLTFISR------CLKGPRVWPILGSLPGLIENCERMHDWISDN 62

Query: 94  L----NLYRTVVVPMPFTT-----YTYIADPANVQHILKTNFANYPKGDVYHSYMEVLLG 153
           L      Y+T +  +PF        T   DP N++HILK  F NYPKG  + +    LLG
Sbjct: 63  LRACSGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLG 122

Query: 154 DGIFNVDGELWRKQRKTASFEFASKNLRD-FSTLVFRDYGLKLSTILTRASLLNQQIDMQ 202
            GIFN DG+ W  QRKTA+ EF ++ LR   +  V R   L+   IL  A L ++ ID+Q
Sbjct: 123 QGIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQ 176

[Close]

BLAST of Cucsat.G17751 vs. NCBI nr
Match: XP_004138991.2 (cytochrome P450 704B1 [Cucumis sativus] >KGN61391.1 hypothetical protein Csa_006668 [Cucumis sativus])

HSP 1 Score: 414 bits (1063), Expect = 2.44e-140
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK
Sbjct: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQ 201
           GLKLSTILTRASLLNQQIDMQ
Sbjct: 181 GLKLSTILTRASLLNQQIDMQ 201

[Close]

BLAST of Cucsat.G17751 vs. NCBI nr
Match: XP_008441698.1 (PREDICTED: cytochrome P450 704B1 [Cucumis melo])

HSP 1 Score: 386 bits (991), Expect = 1.90e-129
Identity = 187/201 (93.03%), Postives = 195/201 (97.01%), Query Frame = 0


Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRA+SEEEW+MRSSNLRRS VV LVL +I I++ CWIIIVHRWN+RKK
Sbjct: 1   MGISLLEWCNNNNRANSEEEWHMRSSNLRRSVVVVLVLGVITIVT-CWIIIVHRWNERKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQ 201
            LKLSTILTRASLLNQQ+DMQ
Sbjct: 181 SLKLSTILTRASLLNQQLDMQ 200

[Close]

BLAST of Cucsat.G17751 vs. NCBI nr
Match: KAA0032319.1 (cytochrome P450 704B1 [Cucumis melo var. makuwa] >TYJ99426.1 cytochrome P450 704B1 [Cucumis melo var. makuwa])

HSP 1 Score: 303 bits (777), Expect = 9.30e-98
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 54  RWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADP 113
           +WN+RKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADP
Sbjct: 6   KWNERKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADP 65

Query: 114 ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 173
           ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS
Sbjct: 66  ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 125

Query: 174 TLVFRDYGLKLSTILTRASLLNQQIDMQ 201
           TLVFRDY LKLSTILTRASLLNQQ+DMQ
Sbjct: 126 TLVFRDYSLKLSTILTRASLLNQQLDMQ 153

[Close]

BLAST of Cucsat.G17751 vs. NCBI nr
Match: XP_022946230.1 (cytochrome P450 704B1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 278 bits (710), Expect = 2.77e-88
Identity = 137/184 (74.46%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQ 77
           ++EWY       +SGV+ +   ++      W+II HRWNQRK    GPKTWP++GAA+EQ
Sbjct: 3   DQEWYSS-----KSGVLGVATCLVW----TWMII-HRWNQRKNT--GPKTWPLLGAALEQ 62

Query: 78  FVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYM 137
            +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSYM
Sbjct: 63  LLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYM 122

Query: 138 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQ 197
           EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQQ
Sbjct: 123 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQ 174

Query: 198 IDMQ 201
           +D+Q
Sbjct: 183 VDVQ 174

[Close]

BLAST of Cucsat.G17751 vs. NCBI nr
Match: KAG7029959.1 (Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278 bits (710), Expect = 1.48e-87
Identity = 137/184 (74.46%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQ 77
           ++EWY       +SGV+ +   ++      W+II HRWNQRK    GPKTWP++GAA+EQ
Sbjct: 17  DQEWYSS-----KSGVLGVATCLVW----TWMII-HRWNQRKNT--GPKTWPLLGAALEQ 76

Query: 78  FVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYM 137
            +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSYM
Sbjct: 77  LLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYM 136

Query: 138 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQ 197
           EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQQ
Sbjct: 137 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQ 188

Query: 198 IDMQ 201
           +D+Q
Sbjct: 197 VDVQ 188

[Close]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL
Match: A0A0A0LN46 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 1.18e-140
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK
Sbjct: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQ 201
           GLKLSTILTRASLLNQQIDMQ
Sbjct: 181 GLKLSTILTRASLLNQQIDMQ 201

[Close]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL
Match: A0A1S3B4S2 (cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1)

HSP 1 Score: 386 bits (991), Expect = 9.18e-130
Identity = 187/201 (93.03%), Postives = 195/201 (97.01%), Query Frame = 0


Query: 1   MGISLLEWCNNNNRADSEEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKK 60
           MGISLLEWCNNNNRA+SEEEW+MRSSNLRRS VV LVL +I I++ CWIIIVHRWN+RKK
Sbjct: 1   MGISLLEWCNNNNRANSEEEWHMRSSNLRRSVVVVLVLGVITIVT-CWIIIVHRWNERKK 60

Query: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHIL 120
           MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADPANVQHIL
Sbjct: 61  MRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADPANVQHIL 120

Query: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180
           KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY
Sbjct: 121 KTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDY 180

Query: 181 GLKLSTILTRASLLNQQIDMQ 201
            LKLSTILTRASLLNQQ+DMQ
Sbjct: 181 SLKLSTILTRASLLNQQLDMQ 200

[Close]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL
Match: A0A5D3BJX9 (Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold316G00130 PE=3 SV=1)

HSP 1 Score: 303 bits (777), Expect = 4.50e-98
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 54  RWNQRKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADP 113
           +WN+RKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLY+TVVVPMPFTTYTYIADP
Sbjct: 6   KWNERKKMRRGPKTWPIVGAAIEQFVNYHRMHDWILGYLNLYKTVVVPMPFTTYTYIADP 65

Query: 114 ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 173
           ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS
Sbjct: 66  ANVQHILKTNFANYPKGDVYHSYMEVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS 125

Query: 174 TLVFRDYGLKLSTILTRASLLNQQIDMQ 201
           TLVFRDY LKLSTILTRASLLNQQ+DMQ
Sbjct: 126 TLVFRDYSLKLSTILTRASLLNQQLDMQ 153

[Close]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL
Match: A0A6J1G394 (cytochrome P450 704B1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450376 PE=3 SV=1)

HSP 1 Score: 278 bits (710), Expect = 1.34e-88
Identity = 137/184 (74.46%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQ 77
           ++EWY       +SGV+ +   ++      W+II HRWNQRK    GPKTWP++GAA+EQ
Sbjct: 3   DQEWYSS-----KSGVLGVATCLVW----TWMII-HRWNQRKNT--GPKTWPLLGAALEQ 62

Query: 78  FVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYM 137
            +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSYM
Sbjct: 63  LLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYM 122

Query: 138 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQ 197
           EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQQ
Sbjct: 123 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQ 174

Query: 198 IDMQ 201
           +D+Q
Sbjct: 183 VDVQ 174

[Close]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL
Match: A0A6J1ICQ4 (cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1)

HSP 1 Score: 278 bits (710), Expect = 8.40e-88
Identity = 137/184 (74.46%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 18  EEEWYMRSSNLRRSGVVALVLVMIAIISACWIIIVHRWNQRKKMRRGPKTWPIVGAAIEQ 77
           ++EWY       +SGV+ +   ++      W+II HRWNQRK    GPKTWP++GAA+EQ
Sbjct: 3   DQEWYSS-----KSGVLGVATCLVW----TWMII-HRWNQRKNT--GPKTWPLLGAALEQ 62

Query: 78  FVNYHRMHDWILGYLNLYRTVVVPMPFTTYTYIADPANVQHILKTNFANYPKGDVYHSYM 137
            +NYHRMHDW+LGYLNL +TVVVPMPFTTYTYIADPANVQHILKTNF NYPKGD YHSYM
Sbjct: 63  LLNYHRMHDWLLGYLNLSKTVVVPMPFTTYTYIADPANVQHILKTNFPNYPKGDAYHSYM 122

Query: 138 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSTLVFRDYGLKLSTILTRASLLNQQ 197
           EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFS LVFR+Y LKLS ILTRASL NQQ
Sbjct: 123 EVLLGDGIFNVDGELWRKQRKTASFEFASKNLRDFSALVFREYSLKLSDILTRASLHNQQ 174

Query: 198 IDMQ 201
           +D+Q
Sbjct: 183 VDIQ 174

The following BLAST results are available for this feature:

BLAST of Cucsat.G17751 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C788	7.3e-65	69.32	Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1	[more]
Q50EK3	1.3e-32	42.86	Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1	[more]
Q9CAD6	5.1e-26	38.25	Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1	[more]
O23066	1.1e-23	35.20	Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1	[more]
O80823	5.3e-23	36.67	Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1	[more]

BLAST of Cucsat.G17751 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138991.2	2.44e-140	100.00	cytochrome P450 704B1 [Cucumis sativus] >KGN61391.1 hypothetical protein Csa_006...	[more]
XP_008441698.1	1.90e-129	93.03	PREDICTED: cytochrome P450 704B1 [Cucumis melo]	[more]
KAA0032319.1	9.30e-98	96.62	cytochrome P450 704B1 [Cucumis melo var. makuwa] >TYJ99426.1 cytochrome P450 704...	[more]
XP_022946230.1	2.77e-88	74.46	cytochrome P450 704B1 isoform X2 [Cucurbita moschata]	[more]
KAG7029959.1	1.48e-87	74.46	Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cucsat.G17751 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LN46	1.18e-140	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108690 PE=3 SV=1	[more]
A0A1S3B4S2	9.18e-130	93.03	cytochrome P450 704B1 OS=Cucumis melo OX=3656 GN=LOC103485770 PE=3 SV=1	[more]
A0A5D3BJX9	4.50e-98	96.62	Cytochrome P450 704B1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold31...	[more]
A0A6J1G394	1.34e-88	74.46	cytochrome P450 704B1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450376 P...	[more]
A0A6J1ICQ4	8.40e-88	74.46	cytochrome P450 704B1 OS=Cucurbita maxima OX=3661 GN=LOC111471352 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 52..307 e-value: 7.8E-35 score: 122.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 64..296
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 64..298 e-value: 2.7E-17 score: 62.7
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 37..299
None	No IPR available	PANTHER	PTHR24296:SF8	CYTOCHROME P450 704B1	coord: 37..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G17751.T1	Cucsat.G17751.T1	mRNA
Cucsat.G17751.T3	Cucsat.G17751.T3	mRNA
Cucsat.G17751.T2	Cucsat.G17751.T2	mRNA
Cucsat.G17751.T4	Cucsat.G17751.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen