Cucsat.G1771 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G1771
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome P450
Location	ctg1002: 1952094 .. 1953906 (-)
RNA-Seq Expression	Cucsat.G1771
Synteny	Cucsat.G1771

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTTGGAGAATACTCAATTATAATGTCTGTCCTTCTCCATTAGGTATATTTATATATCAACACAAATTGAAATCATACCCACAAAAAAAAAACACCATGGATTTAGACACTTACGTCCTCTATTCTTCTCTCTCACTTTTCTTTCTTCTTCTTGCTTTCAAATTCATCTCTCGATCTCTTCGACTTCGCAATAACTTACCGCCAAGTCCACCCTCTTTTCCGGTCATCGGACATCTCCATCTCTTGAAAAAGCCAATCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCGTTGGAAAATACATCGGCTATAATTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCGATTCATCAATTCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTATGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATGTCGAAGGGTATTGAGAGGAAATTAATACAACTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAGAAGGAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAGATAATCAAAGGATTTATATTGGTAATTAATAAATTCTCCCACTTCTCTATTTTATTTATCATTTTTTTTTGGAAGAAAGGTGGATTTCCACAAATTTTTAGTTAAAAAGTTGTAATAAACTTACCAGAAATATAATAAATTTTGTAGATATTACTAACGGCTGGATCCGACACATCCGCGGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTGTTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCGTCTCTGAGACTCTTAGGTTGAATCCTGCGGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTATGATGTGCCACGAGACACAATGGTACTAGTCAATGCTTGGGCCATACATAGAGATTCAAACGAGTGGGAGGATTGCATGAGTTTTAAACCAGAGCGATACGAAAAATCAGAAGCTGTAGAGATACACAAGTTTTTGCCTTTTGGATTAGGAAGGCGTGCATGTCCTGGTTCTGCCATGGCACATAGAGTGATGGGTCTTACTTTAGCAACTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGAAGAAGACATTGACATGAAGGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCCATCATCAACAACATTTTTTACTAATAAATATCTATTTCTAGTAATTTACATGTACTTGTGTATTTTTTTACAAGAGATAATTGCAAATTTAACAATTAGATTCAAAATAAT

Coding sequence (CDS)

ACTTGGAGAATACTCAATTATAATGTCTGTCCTTCTCCATTAGGTATATTTATATATCAACACAAATTGAAATCATACCCACAAAAAAAAAACACCATGGATTTAGACACTTACGTCCTCTATTCTTCTCTCTCACTTTTCTTTCTTCTTCTTGCTTTCAAATTCATCTCTCGATCTCTTCGACTTCGCAATAACTTACCGCCAAGTCCACCCTCTTTTCCGGTCATCGGACATCTCCATCTCTTGAAAAAGCCAATCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCGTTGGAAAATACATCGGCTATAATTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCGATTCATCAATTCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTATGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATGTCGAAGGGTATTGAGAGGAAATTAATACAACTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAGAAGGAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAGATAATCAAAGGATTTATATTGATATTACTAACGGCTGGATCCGACACATCCGCGGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTGTTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCGTCTCTGAGACTCTTAGGTTGAATCCTGCGGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTATGATGTGCCACGAGACACAATGGTACTAGTCAATGCTTGGGCCATACATAGAGATTCAAACGAGTGGGAGGATTGCATGAGTTTTAAACCAGAGCGATACGAAAAATCAGAAGCTGTAGAGATACACAAGTTTTTGCCTTTTGGATTAGGAAGGCGTGCATGTCCTGGTTCTGCCATGGCACATAGAGTGATGGGTCTTACTTTAGCAACTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGAAGAAGACATTGACATGAAGGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCCATCATCAACAACATTTTTTACTAA

Protein sequence

TWRILNYNVCPSPLGIFIYQHKLKSYPQKKNTMDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIFY

Homology

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BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 1.1e-162
Identity = 283/502 (56.37%), Postives = 371/502 (73.90%), Query Frame = 0


Query: 35  LDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSNLS 94
           + T+ L   +SLFFL++  K    + R   NLPP P   P+IG+LH LK+P+H TF  LS
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60

Query: 95  AKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPY 154
            KYG + SL  GS+L VV+SS    ++CFTKND+VLANRP  L GKYIGYN TT+  +PY
Sbjct: 61  QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120

Query: 155 GDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELT 214
           GDHWRNLRRI +IEI S  R+N F +IRRDE+MR+++KL+  S + F++VE++   SE+T
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180

Query: 215 FNITMRMAAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGI 274
           FN  MRM +GKRYYG   DV++ EEAR FR +IKE+V++GG +N GDFL  L W    G+
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240

Query: 275 ERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILIL 334
           E++L ++ K+ DAFLQGLID+HR  K    NTMIDHLL+ QQS+P++Y DQIIKG ++++
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300

Query: 335 LTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSE 394
           L AG+DTS+VT+EWA+S+LLN+P+++KKAK E+DT IG +R V+E DISKLPYLQ IV E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360

Query: 395 TLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEK 454
           TLRL+ AAP+LVPH ++ D  + GY++P++T+++VNAW IHRD N W D   FKPER+EK
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420

Query: 455 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIM 514
               E++K L FGLGRRACPG  ++ R  GLTL  LIQCFEWK+IGEE IDM E  G   
Sbjct: 421 EG--EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITA 480

Query: 515 TKMVPLEAMCKAR-PI-INNIF 532
            K   L AMCK R P+ IN++F
Sbjct: 481 GKKTSLNAMCKVRHPLKINDVF 499

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BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 1.2e-161
Identity = 282/494 (57.09%), Postives = 373/494 (75.51%), Query Frame = 0


Query: 39  VLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPP-SFPVIGHLHLLKKPIHRTFSNLSAKY 98
           +LY+ L++  L ++ K    S R R NLPPSP  + PVIGHLHL+ K +HR+  +LS KY
Sbjct: 6   LLYTFLAVVLLSISLKLFPVS-RRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 65

Query: 99  GPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDH 158
           G V SL+LG++L +V+SS AA E+CFTKND+V ANRP  ++GKYIGYNYTTM+G+PYG+H
Sbjct: 66  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 125

Query: 159 WRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNI 218
           WRNLRR+ A+EIFS   +N+F  IR DEV +++  L  +S   F KVE++S +SEL+FN+
Sbjct: 126 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 185

Query: 219 TMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKLI 278
           TMRM AGKRY+G+DV + +EA+ FR LI E+    G SNPGDF+P L W+  K  E+K+ 
Sbjct: 186 TMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 245

Query: 279 QLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGS 338
           ++ +++DAFLQ LI + R  K     TMIDHLLSLQ+S+P++Y DQIIKG I++LL AG+
Sbjct: 246 KISQEMDAFLQRLIHESRINK--NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGT 305

Query: 339 DTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLN 398
           DTSAVT+EWA+S LLN+P+ L+KA+ EI+TQ+G  RL+EE D+ KL YL  I+SET RL 
Sbjct: 306 DTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLC 365

Query: 399 PAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVE 458
           PAAPMLVPH +++DCK+ GYDVP+ T++LVNAWAIHRD   W++   FKPER+   E +E
Sbjct: 366 PAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVE-LE 425

Query: 459 IHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVP 518
             K +PFG+GRR+CPGS +A RV+GLTL  LIQCFEWK+IGE  IDM E SG  M K  P
Sbjct: 426 PSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQP 485

Query: 519 LEAMCKARPIINNI 531
           LEA+CK R I++ +
Sbjct: 486 LEALCKPRNILHKV 494

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BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 556.6 bits (1433), Expect = 3.0e-157
Identity = 267/493 (54.16%), Postives = 370/493 (75.05%), Query Frame = 0


Query: 43  SLSLFFLLLAFKF-ISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSNLSAKYGPVM 102
           S S+F+L L F F I    R   NLPP PPS P+IG+LH LK+P+HRTF  LS KYG V 
Sbjct: 8   SYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVF 67

Query: 103 SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 162
           SL  GS+L VV+SS++  + CFTKNDVVLANRPR L GKYI YNYTT+    YG+HWRNL
Sbjct: 68  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 127

Query: 163 RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 222
           RRI A+++ S  RIN F+ IRRDE  R++ +L+ +S   F+++E+ S + ++TFN  MRM
Sbjct: 128 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 187

Query: 223 AAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKLIQL 282
            +GKRYYGE  D ++ +EA +FR+++ E++ + G +N  DF+P+L ++  + +E++L  +
Sbjct: 188 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 247

Query: 283 GKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDT 342
             K DAFL+GLI++HR TK+E  NTMIDHLL+LQ S+P++Y DQIIKG  L +L AG+D+
Sbjct: 248 SGKTDAFLRGLIEEHR-TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDS 307

Query: 343 SAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPA 402
           SAVT+EW++S+LLN+P+VLKK K+E+DT +GQ+RLV+ESD+ KL YL+ +++ETLRL   
Sbjct: 308 SAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTP 367

Query: 403 APMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH 462
           AP+L+PH  +++C I GY VP+DT+VL+NAWAIHRD   W +  +FKPER+EK    E+ 
Sbjct: 368 APLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG--ELE 427

Query: 463 KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLE 522
           K + FG+GRRACPG  +A R + +TLA LIQCF+WK I  + ID+ E  G  +TK+VPL+
Sbjct: 428 KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLK 487

Query: 523 AMCKARPIINNIF 532
           AMCK+RP+IN +F
Sbjct: 488 AMCKSRPVINKVF 497

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BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 8.8e-157
Identity = 280/499 (56.11%), Postives = 370/499 (74.15%), Query Frame = 0


Query: 39  VLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSF-PVIGHLHLLKKPIHRTFSNLSAKY 98
           VLYS  SL FL+++FKF+      + NLPPSPP + P+IGHL LLK PIHRT  + S   
Sbjct: 9   VLYSIFSLIFLIISFKFLKPK---KQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 68

Query: 99  -----GPVMSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIG 158
                G VMSLRLGS+L  V+SS   A E+CF KNDVVLANRP++++GK++GYN T MI 
Sbjct: 69  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 128

Query: 159 APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMS 218
           APYGDHWRNLRR+  IEIFS  R+N F  +R DEV R++ +LS  +  + + VE++  + 
Sbjct: 129 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 188

Query: 219 ELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGI 278
           +LTFN  MRM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  D++P+L   S   
Sbjct: 189 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS-SY 248

Query: 279 ERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILIL 338
           E ++ +LG++ D FLQGLIDD R  +E G  TMIDHLL LQ+S+ ++Y DQIIKG ILI+
Sbjct: 249 ENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIKGIILIM 308

Query: 339 LTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSE 398
           + AG++TSAVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+S+LPYL+ IV E
Sbjct: 309 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 368

Query: 399 TLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEK 458
           TLRL+PA P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+D  SFKPER+EK
Sbjct: 369 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 428

Query: 459 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIM 518
            E  E  K L FGLGRRACPGS +A R++GL L +LIQCFEW+++G  ++DMKE  G  +
Sbjct: 429 EE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTV 488

Query: 519 TKMVPLEAMCKARPIINNI 531
            K +PL+A+CKARP ++ I
Sbjct: 489 PKAIPLKAICKARPFLHKI 500

[Close]

BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 5.7e-156
Identity = 264/497 (53.12%), Postives = 366/497 (73.64%), Query Frame = 0


Query: 41  YSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSNLSAKYGPV 100
           YS LSL F++     +  + R   NLPP PP+ P+IG+LH LK P+HRTF+ LS  YG +
Sbjct: 5   YSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDI 64

Query: 101 MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 160
            SL  GS+L VV+SS +   +CFTKND++LANRPR L GKYI YNYTT+  A YGDHWRN
Sbjct: 65  FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 124

Query: 161 LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQ---FSKVEIQSAMSELTFNI 220
           LRRI  I++ S +R+N F  +RRDE  R+++KL  + + +   F+KVE++  ++E+TFN 
Sbjct: 125 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 184

Query: 221 TMRMAAGKRYYGE--DVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERK 280
            MRM +GKRYYG+  DV++ EEA++FRE+I E++++ G +N GDFLP+L  +    +E++
Sbjct: 185 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 244

Query: 281 LIQLGKKVDAFLQGLIDDHRKTK-EEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLT 340
             ++ K+ +AFL+GLI++HR+        TMIDHLL L +S+P++Y+D +IKG I  +L 
Sbjct: 245 CKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLL 304

Query: 341 AGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETL 400
           AG+DTSAVT+EW +S LLN+P+VLKKAKEE+DTQIG+ +LV+E D+SKLPYLQ I+SETL
Sbjct: 305 AGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETL 364

Query: 401 RLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSE 460
           RL+P AP+L+PHY++ DC I  ++VP+DT++L N W IHRD   W D +SFKPER+EK E
Sbjct: 365 RLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEE 424

Query: 461 AVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTK 520
             E++K + FGLGRRACPG ++A R +G T+  LIQCFEW++  EE +DM E  G  M  
Sbjct: 425 --EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPM 484

Query: 521 MVPLEAMCKARPIINNI 531
            +PL AMCKA PI N++
Sbjct: 485 KIPLRAMCKALPIANDV 499

[Close]

BLAST of Cucsat.G1771 vs. NCBI nr
Match: XP_004141996.2 (cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_003503 [Cucumis sativus])

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET
Sbjct: 301 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT
Sbjct: 421 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCKARPIINNIFY
Sbjct: 481 KMVPLEAMCKARPIINNIFY 500

[Close]

BLAST of Cucsat.G1771 vs. NCBI nr
Match: XP_008440331.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 471/500 (94.20%), Postives = 488/500 (97.60%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKP HRTF N
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPPHRTFLN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANRPRLL+GKYIGYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHR TKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSAVTMEWAL HLLNNPQVLK A++E+DTQIGQERLVEESDI KLPYLQRI+ ET
Sbjct: 301 TAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERLVEESDIPKLPYLQRIIYET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYA+NDCKICGYDVPR+TMVL+NAWAIHRDSNEWED MSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           +A+E+HKFLPFG+GRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDM+ELSGA+MT
Sbjct: 421 KAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCK RPIINNI Y
Sbjct: 481 KMVPLEAMCKTRPIINNILY 500

[Close]

BLAST of Cucsat.G1771 vs. NCBI nr
Match: TYK12853.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 468/500 (93.60%), Postives = 487/500 (97.40%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVL SSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKP+HRTF N
Sbjct: 1   MDLDTYVLCSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPLHRTFLN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANRPRLL+GKYIGYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHR TKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSA+TMEWAL HLLNNPQVLKKA++E+DTQIGQE LVEESDI KLPYLQRI+ ET
Sbjct: 301 TAGSDTSAMTMEWALCHLLNNPQVLKKARDELDTQIGQEILVEESDIPKLPYLQRIIYET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYA+NDCKICGYDVPR+TMVL+NAWAIHRDSNEWED MSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           +A+E+HKFLPFG+GRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDM+ELSGA+MT
Sbjct: 421 KAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCK R IINNI Y
Sbjct: 481 KMVPLEAMCKTRSIINNILY 500

[Close]

BLAST of Cucsat.G1771 vs. NCBI nr
Match: XP_022949637.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 835 bits (2158), Expect = 3.03e-302
Identity = 410/498 (82.33%), Postives = 453/498 (90.96%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDL T+ LYS  S+ FLL AF  I+RSLRLR NLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLHTF-LYSFFSILFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
            YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKLS NSIHQ+SKVE+QSA+SE
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           +TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GGVSNPGDF P+LNW+SKG E
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKL +LGKKVDAFLQGLIDDHR+ KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAG+DTSAVT+EWAL HLLNNP+VLKKA++EIDTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRL PAAPML+PHYA NDC I GYDVPRDTM+LVNAWAIHRD NEWED +SFKPERYEKS
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           E VE+HK L FGLGRRACPGSAMAHR+MGLTL  LIQCFEW+KIG ED+DM E  GA MT
Sbjct: 421 E-VEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEWEKIGAEDLDMNERVGATMT 480

Query: 513 KMVPLEAMCKARPIINNI 530
           KMVPLEAMC+ARP ++N+
Sbjct: 481 KMVPLEAMCRARPTVDNL 496

[Close]

BLAST of Cucsat.G1771 vs. NCBI nr
Match: XP_022977458.1 (cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 835 bits (2157), Expect = 4.47e-302
Identity = 410/497 (82.49%), Postives = 452/497 (90.95%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDL T+ LYS  S  FLL AF  I+RSLR R NLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLHTF-LYSFFSPLFLLFAFNLITRSLRPRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
            YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKLS NSIHQ+SKVE+QSA+SE
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           +TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GGVSNPGDF P+LNW+SKG E
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLI+LGKKVDAFLQGLIDDHR+ KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAG+DTSAVT+EWALSHLLNNP+VLKKAK+EIDTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRL PAAPML+PHYA NDC ICGYDVPRDTM+LVNAWAIHRD NEWED +SFKPERYEKS
Sbjct: 361 LRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           E VE+HK L FG+GRRACPGSAMAHR+MGLTL  LIQCFEW++IG ED+DM E  GA MT
Sbjct: 421 E-VEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATMT 480

Query: 513 KMVPLEAMCKARPIINN 529
           KMVPLEAMC+ARP ++ 
Sbjct: 481 KMVPLEAMCRARPTVDG 495

[Close]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL
Match: A0A0A0KFV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1)

HSP 1 Score: 998 bits (2579), Expect = 0.0
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET
Sbjct: 301 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT
Sbjct: 421 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCKARPIINNIFY
Sbjct: 481 KMVPLEAMCKARPIINNIFY 500

[Close]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL
Match: A0A1S3B0W6 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1)

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 471/500 (94.20%), Postives = 488/500 (97.60%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKP HRTF N
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPPHRTFLN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANRPRLL+GKYIGYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHR TKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSAVTMEWAL HLLNNPQVLK A++E+DTQIGQERLVEESDI KLPYLQRI+ ET
Sbjct: 301 TAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERLVEESDIPKLPYLQRIIYET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYA+NDCKICGYDVPR+TMVL+NAWAIHRDSNEWED MSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           +A+E+HKFLPFG+GRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDM+ELSGA+MT
Sbjct: 421 KAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCK RPIINNI Y
Sbjct: 481 KMVPLEAMCKTRPIINNILY 500

[Close]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL
Match: A0A5D3CLS1 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004450 PE=3 SV=1)

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 468/500 (93.60%), Postives = 487/500 (97.40%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDLDTYVL SSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKP+HRTF N
Sbjct: 1   MDLDTYVLCSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPLHRTFLN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANRPRLL+GKYIGYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
           PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           LTFNITMRMAAGKRYYGEDVTNEEEARRFR LIKEIVAVGGVSNPGDFLPVLNWMSKGIE
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLIQLGKKVDAFLQGLIDDHR TKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAGSDTSA+TMEWAL HLLNNPQVLKKA++E+DTQIGQE LVEESDI KLPYLQRI+ ET
Sbjct: 301 TAGSDTSAMTMEWALCHLLNNPQVLKKARDELDTQIGQEILVEESDIPKLPYLQRIIYET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRLNPAAPMLVPHYA+NDCKICGYDVPR+TMVL+NAWAIHRDSNEWED MSFKPERYEKS
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           +A+E+HKFLPFG+GRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDM+ELSGA+MT
Sbjct: 421 KAMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMT 480

Query: 513 KMVPLEAMCKARPIINNIFY 532
           KMVPLEAMCK R IINNI Y
Sbjct: 481 KMVPLEAMCKTRSIINNILY 500

[Close]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL
Match: A0A6J1GCP2 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=1)

HSP 1 Score: 835 bits (2158), Expect = 1.47e-302
Identity = 410/498 (82.33%), Postives = 453/498 (90.96%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDL T+ LYS  S+ FLL AF  I+RSLRLR NLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLHTF-LYSFFSILFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
            YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKLS NSIHQ+SKVE+QSA+SE
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           +TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GGVSNPGDF P+LNW+SKG E
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKL +LGKKVDAFLQGLIDDHR+ KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAG+DTSAVT+EWAL HLLNNP+VLKKA++EIDTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRL PAAPML+PHYA NDC I GYDVPRDTM+LVNAWAIHRD NEWED +SFKPERYEKS
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           E VE+HK L FGLGRRACPGSAMAHR+MGLTL  LIQCFEW+KIG ED+DM E  GA MT
Sbjct: 421 E-VEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEWEKIGAEDLDMNERVGATMT 480

Query: 513 KMVPLEAMCKARPIINNI 530
           KMVPLEAMC+ARP ++N+
Sbjct: 481 KMVPLEAMCRARPTVDNL 496

[Close]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL
Match: A0A6J1IQ18 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1)

HSP 1 Score: 835 bits (2157), Expect = 2.16e-302
Identity = 410/497 (82.49%), Postives = 452/497 (90.95%), Query Frame = 0


Query: 33  MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 92
           MDL T+ LYS  S  FLL AF  I+RSLR R NLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLHTF-LYSFFSPLFLLFAFNLITRSLRPRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 93  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 152
           LSAKYGPVMSLRLGS LAVV+SSS+AVE+CFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 153 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 212
            YGDHWRNLRRIGAIEIFS SRINKF DIRRDEV R+VRKLS NSIHQ+SKVE+QSA+SE
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 213 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 272
           +TFNI+MRMAAGKRYYGEDVT++EEARRFRELIKEIVA GGVSNPGDF P+LNW+SKG E
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 273 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 332
           RKLI+LGKKVDAFLQGLIDDHR+ KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 333 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 392
           TAG+DTSAVT+EWALSHLLNNP+VLKKAK+EIDTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 393 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKS 452
           LRL PAAPML+PHYA NDC ICGYDVPRDTM+LVNAWAIHRD NEWED +SFKPERYEKS
Sbjct: 361 LRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 453 EAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMT 512
           E VE+HK L FG+GRRACPGSAMAHR+MGLTL  LIQCFEW++IG ED+DM E  GA MT
Sbjct: 421 E-VEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATMT 480

Query: 513 KMVPLEAMCKARPIINN 529
           KMVPLEAMC+ARP ++ 
Sbjct: 481 KMVPLEAMCRARPTVDG 495

[Close]

BLAST of Cucsat.G1771 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 576.6 bits (1485), Expect = 2.0e-164
Identity = 286/490 (58.37%), Postives = 373/490 (76.12%), Query Frame = 0


Query: 45  SLFFLLLAFKFISRSLRLRNNLPPSPP-SFPVIGHLHLLKKPIHRTFSNLSAKY--GPVM 104
           S+ F++L+  ++   L+ + NLPPSP  S PVIGHL LLK PIHRTF +LS      P+ 
Sbjct: 9   SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68

Query: 105 SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 164
           SLRLG++L  V SS +  E+CFTKNDVVLANRP  ++ K++ Y+YTTMI A YGDHWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 165 RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 224
           RRIG++EIFS  R+N F  IR+DE+ R+V +LS N   +F KV+++S +S+LTFN  +RM
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 225 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 284
            AGKRYYG+ V ++ EA+R R+LI ++VA  G  N  D+LPVL  +S   E ++ +L  +
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS-DYETRVKKLAGR 248

Query: 285 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 344
           +D FLQGL+D+ R+ KE+G NTMIDHLL+LQ+S+PD++ D+IIKG +L L+ AG+DTSAV
Sbjct: 249 LDEFLQGLVDEKREAKEKG-NTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAV 308

Query: 345 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 404
           T+EWALS++LN+P VL KA++EID +IG +RL++ESDIS LPYLQ IVSETLRL PAAPM
Sbjct: 309 TLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPM 368

Query: 405 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 464
           L+PH A+ DCK+ GYD+PR T++L N WAIHRD   W+D MSFKPER+EK    E  K +
Sbjct: 369 LLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG--EAQKLM 428

Query: 465 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMC 524
           PFGLGRRACPGS +AHR++ LTL +LIQC EW+KIGEE +DM E  G  M K  PLEAMC
Sbjct: 429 PFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMC 488

Query: 525 KARPIINNIF 532
           +ARP +  IF
Sbjct: 489 RARPSVVKIF 493

[Close]

BLAST of Cucsat.G1771 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 559.3 bits (1440), Expect = 3.3e-159
Identity = 275/490 (56.12%), Postives = 363/490 (74.08%), Query Frame = 0


Query: 45  SLFFLLLAFKFISRSLRLRNNLPPSPP-SFPVIGHLHLLKKPIHRTFSNLSAKYG--PVM 104
           +  F+ L+  FI   ++ R NLPPSP  + PVIGHL LLK P+HR F ++S   G  P++
Sbjct: 9   TFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPII 68

Query: 105 SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 164
           SLRLG++L  V+SS +  E+CFTKNDVVLANR   L  K+I Y  TT++ A YGDHWRNL
Sbjct: 69  SLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNL 128

Query: 165 RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 224
           RRIGA+EIFS  R+N F+ IRRDE+ R++  LS NS  +F+KVE++S  S LTFN  +RM
Sbjct: 129 RRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRM 188

Query: 225 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 284
            AGK YYG+   ++ EA+R RELI E +   G  N  D+LP+L W++ G E+++ ++  +
Sbjct: 189 LAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWIT-GSEKRIKKIASR 248

Query: 285 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 344
           +D FLQGL+D+ R+ KE+ +NTM+DHLL LQ+++P++Y D IIKG +L L+ AG+DTSAV
Sbjct: 249 LDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAV 308

Query: 345 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 404
           T+EW LS LLN+PQ+L KA++EID ++G  RLVEESD+S LPYLQ IVSE+LRL PA+P+
Sbjct: 309 TLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPL 368

Query: 405 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 464
           LVPH A+ DCK+ GY +PR TM+L NAWAIHRD   W+D  SFKPER+EK    E  K L
Sbjct: 369 LVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG--EAQKLL 428

Query: 465 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKE-LSGAIMTKMVPLEAM 524
            FGLGRRACPGS +A R+  LT+ +LIQCFEW++IGEE++DM E   G IM K +PL AM
Sbjct: 429 GFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAM 488

Query: 525 CKARPIINNI 531
           CKARP++  I
Sbjct: 489 CKARPVVGKI 495

[Close]

BLAST of Cucsat.G1771 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 555.1 bits (1429), Expect = 6.3e-158
Identity = 280/499 (56.11%), Postives = 370/499 (74.15%), Query Frame = 0


Query: 39  VLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSF-PVIGHLHLLKKPIHRTFSNLSAKY 98
           VLYS  SL FL+++FKF+      + NLPPSPP + P+IGHL LLK PIHRT  + S   
Sbjct: 9   VLYSIFSLIFLIISFKFLKPK---KQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 68

Query: 99  -----GPVMSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIG 158
                G VMSLRLGS+L  V+SS   A E+CF KNDVVLANRP++++GK++GYN T MI 
Sbjct: 69  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 128

Query: 159 APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMS 218
           APYGDHWRNLRR+  IEIFS  R+N F  +R DEV R++ +LS  +  + + VE++  + 
Sbjct: 129 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 188

Query: 219 ELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGI 278
           +LTFN  MRM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  D++P+L   S   
Sbjct: 189 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS-SY 248

Query: 279 ERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILIL 338
           E ++ +LG++ D FLQGLIDD R  +E G  TMIDHLL LQ+S+ ++Y DQIIKG ILI+
Sbjct: 249 ENRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIKGIILIM 308

Query: 339 LTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSE 398
           + AG++TSAVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+S+LPYL+ IV E
Sbjct: 309 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 368

Query: 399 TLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEK 458
           TLRL+PA P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+D  SFKPER+EK
Sbjct: 369 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 428

Query: 459 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIM 518
            E  E  K L FGLGRRACPGS +A R++GL L +LIQCFEW+++G  ++DMKE  G  +
Sbjct: 429 EE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTV 488

Query: 519 TKMVPLEAMCKARPIINNI 531
            K +PL+A+CKARP ++ I
Sbjct: 489 PKAIPLKAICKARPFLHKI 500

[Close]

BLAST of Cucsat.G1771 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 548.5 bits (1412), Expect = 5.9e-156
Identity = 277/500 (55.40%), Postives = 360/500 (72.00%), Query Frame = 0


Query: 35  LDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPP-SFPVIGHLHLLKKPIHRTFSNL 94
           ++T  L  SL  FF+ L+ K +      + NLPPSP   FP+IGHLHLLK P+HR F +L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 95  SAKY--GPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIG 154
           S       + SL LGS+L  V+SS A  E+CFTKNDVVLANRP  LVGK+IGYN TTM+G
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 155 APYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMS 214
           A YGD WRNLRRIG IEIFS  R+N F  IR+DE+ R++  L+ NS H F KVE++    
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 215 ELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGI 274
            LT N  +RM AGKR+YG+   N+ EA+  R+LI E+V  GG  N  D+ P+L +++   
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVT-NY 247

Query: 275 ERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILIL 334
           E+ + +L  +VD FLQ L+++ R  K +G NTMIDHLLSLQ+++PD+Y D IIKG IL++
Sbjct: 248 EKHVKKLAGRVDEFLQSLVNEKRVEKVKG-NTMIDHLLSLQETQPDYYTDVIIKGIILVM 307

Query: 335 LTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSE 394
           + AG+DTSA T+EWA+S+LLN+P+VL+KAK EID QIG +RLVEE DI KLPYLQ IVSE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367

Query: 395 TLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEK 454
           TLRL P APML+PH A+ DC + GYDVPR T++LVNAWAIHRD   WE+   FKPER+EK
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427

Query: 455 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKEL-SGAI 514
               E  K +PFG+GRR+CPGS +A R++ L L +L+QCFEW+++ E+ +DM+E   G  
Sbjct: 428 KG--EDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTT 487

Query: 515 MTKMVPLEAMCKARPIINNI 531
           M K   L+AMCKARPI++ +
Sbjct: 488 MRKATSLQAMCKARPIVHKV 503

[Close]

BLAST of Cucsat.G1771 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 545.8 bits (1405), Expect = 3.8e-155
Identity = 266/486 (54.73%), Postives = 360/486 (74.07%), Query Frame = 0


Query: 48  FLLLAFKFISRSLRLRNNLPPSPP-SFPVIGHLHLLKKPIHRTFSNLSAKYG--PVMSLR 107
           F++L+  F+   ++ + NLPPSP  + PVIGHL LLK P+HR F ++S   G  P++SLR
Sbjct: 12  FIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLR 71

Query: 108 LGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNLRRI 167
           LG++L  V+SS +  E+CFTKNDV+LANR   +  K+I Y  +T++ A Y +HWRNLRRI
Sbjct: 72  LGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRI 131

Query: 168 GAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRMAAG 227
           GA+EIFS  R+N F+ IRRDE+ R++ +L  NS + F+KVE++S  S+LTFN  +RM AG
Sbjct: 132 GALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAG 191

Query: 228 KRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKKVDA 287
           K YYG+   ++ EA+R R LI E ++  G  N  D++P+L W++   E ++ +L  ++D 
Sbjct: 192 KCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-ETRIKKLAGRLDE 251

Query: 288 FLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTME 347
           FLQGL+D+ R+ KE+  NTM+DHLL LQ+++P++Y D+IIKG +L L+  G+DT+AVT+E
Sbjct: 252 FLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLE 311

Query: 348 WALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPMLVP 407
           WALS LLNNP+VL KA++EID  IG +RL+EESDI  LPYLQ IVSETLRL PAAPML+P
Sbjct: 312 WALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLP 371

Query: 408 HYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFLPFG 467
           H A+ DCK+ GYD+PR TM+L NAWAIHRD   W+D  SFKPER+EK    E  K +PFG
Sbjct: 372 HVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG--EAKKLMPFG 431

Query: 468 LGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMCKAR 527
           LGRRACPGS +A R++ L+L +LIQCFEW++IGEE++DM E  G  M K  PLEAMC+AR
Sbjct: 432 LGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRAR 491

Query: 528 PIINNI 531
             +  I
Sbjct: 492 DFVGKI 494

The following BLAST results are available for this feature:

BLAST of Cucsat.G1771 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WNQ8	1.1e-162	56.37	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
W8JMU7	1.2e-161	57.09	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	3.0e-157	54.16	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q9FG65	8.8e-157	56.11	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q6WNQ9	5.7e-156	53.12	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]

BLAST of Cucsat.G1771 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141996.2	0.0	100.00	cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_0035...	[more]
XP_008440331.1	0.0	94.20	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
TYK12853.1	0.0	93.60	cytochrome P450 81E8-like [Cucumis melo var. makuwa]	[more]
XP_022949637.1	3.03e-302	82.33	cytochrome P450 81E8-like [Cucurbita moschata]	[more]
XP_022977458.1	4.47e-302	82.49	cytochrome P450 81E8-like [Cucurbita maxima]	[more]

BLAST of Cucsat.G1771 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFV2	0.0	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1	[more]
A0A1S3B0W6	0.0	94.20	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1	[more]
A0A5D3CLS1	0.0	93.60	Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1GCP2	1.47e-302	82.33	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=...	[more]
A0A6J1IQ18	2.16e-302	82.49	cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1	[more]

BLAST of Cucsat.G1771 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	2.0e-164	58.37	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	3.3e-159	56.12	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT5G36220.1	6.3e-158	56.11	cytochrome p450 81d1	[more]
AT3G28740.1	5.9e-156	55.40	Cytochrome P450 superfamily protein	[more]
AT4G37360.1	3.8e-155	54.73	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 386..404 score: 31.64 coord: 323..340 score: 28.51 coord: 460..470 score: 59.43 coord: 470..493 score: 32.52 coord: 213..231 score: 24.86 coord: 343..369 score: 37.82 coord: 94..113 score: 43.8 coord: 427..451 score: 29.44 coord: 118..139 score: 19.42
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 334..351 score: 36.18 coord: 387..398 score: 35.82 coord: 470..481 score: 28.84 coord: 461..470 score: 60.23
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 67..513 e-value: 6.7E-96 score: 321.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 53..523 e-value: 2.7E-124 score: 417.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 67..524
None	No IPR available	PIRSR	PIRSR000209-1	PIRSR000209-1	coord: 68..505 e-value: 1.4E-41 score: 140.0
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 40..528
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 40..528
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 463..472

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G1771.T1	Cucsat.G1771.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen