Cucsat.G17495 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G17495
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	ctg278: 325108 .. 329054 (-)
RNA-Seq Expression	Cucsat.G17495
Synteny	Cucsat.G17495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTCCATAAAAAATGCAGAATCCAACGATTTCCCGCCATGTTAACCGTAGCGCCAAGAACCCTATCTCCTTCACCGTTTCTTCACATCCCACAAACAAACTCCCATTTCCTCACCTCTTCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAATACCCCTCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGTCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTTTACCGGAAATCCAAGGGCCGTCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTATCTAGAGTTGCGGAAAATATTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGCGGACATCGGCCTTCTCAGTGAGTGCAGAATCTTACTCTTTTAGTTATTTGCTTTAACTGGTTCATTAAAATCTTGATATGAAATATCTTATCATTGTAATATTTTGTATCTATGCATAGAGGTGCTGAGGGATTGATAATGTTTATAATTAAATTTAACAATAACTCAATAGTTTAAGCTCTTGGGTCTTTGAGTGATTAGATATGGTATAGAGTTGATGTGTTTAAACTCTGTAATGTACTAATGTCATCTCCTTCCCACTTAATATTGTTTTTCACTTTTGTTGGGAATTTTATATAAATTTTCAAGAGATAACTGTGGTGGAGTGCTATTGTTATAATTAAATTTATCACAAATTATCAGCTTAAACTTTTTGGGTCAATTATTTAATAAAAAAGATTAGATATAGTTATTTTAGTTTTGATTTTTAGTAGTTGAATTTGGTGTTAGCTCTCAATTAATAGTGTTAGTTACTTGGTGAGTCTTTGTTTCTAGGACTAAGCAATTAGACTTGGAACTGACGGATTGGTTAAACAAAAACTTTAAACTGACTTTTCAGCGGAATGTTTAAAAAACTGAACTGAACTGAACTAGCTAAATTGACAAAACCAGCTTAAATTGAATAAGGGTTGCTAAAAATAACAAACTTTCTCCAAATCATTCTGATCGTTTCTGGTTTTGACTTGCATCCTTACTTGTACCAAGTTTTAAGCCTAAAAGTGAAATGGTTGCTAAGTATATGAATATAGGTGGATAATTATATGAAGATGTGGTAATTAAAATTCTTTTTTCTCCTTTTTCTTGTTTCTCTCTTTTTCTTTCCATTTCTCTCTAGATCTTTAGCTGCATACGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGTGATAGTTGATGGGTTTAGCGTTGTCTGCTAGTGTTGGATGTATCTAGTATGATCATTGATGGTGATTGATCGAAGCTCTCACTATTCAATATCAGGCATTATGGTTTTGTTAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTAAGATAACGACTACTATGATTTTGCTTGAAATGATTAATGAATATGCTTGGATTTGGTGGTTTCTTATTCGTTTAGGTTCTTGATTTCTAGTGTAATTACTTGCAACATTCAATTTTACAAGTTCGGAGATACATTAAGTTTTTTGACTTGTTAGGTTTCTGAGTCACTTGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGCGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAATTTTATTTGTTCTTCTCGTGATTTAATATTTTATCTTGTTTTGATCCAATAATGCAAGAAGTGATCTATCAAAGCCCCCCTTCTCTATGCTTAGGTGGCAAGTCGGGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGTGAGAATACTGGTACTTAAGATGTGATAAATTGTGTGATGAATGTTATTCCACACGGCTTAACTGGTTTTGTTGTCGTGATATTCTCTTGAATGCTTGAAAATAAAGAAATATTGTTGTATACATGAGACACAAGACAAAAAAACAATGATGGGTTATTCTCTATGAGTAGGACATAGCCATGGCGTGGATGTGCTTTGCTAAAATTTATATGTTTTTGAGCTATATCGTTTTTATACAATTTGAAGTCTGTAAGCTTTGTGTGTATAATCTATTTGAAAAAATAAGTTTGGATAGTTTTGTTTCAATATTTATTATTATTATTTTAACAAATTTTTGACACGTGTCTAACAAGTGCAAAAACTTATACTCAATTTGTTTAACTACTGTCCAGCACATGTCTATTTGTCTTGTGGGTACAAGTATATCACACTTGTTCATCATGTTTTGGAAGTGTCCAATACGCGTTGAACATGGACGATTTGTCCAAACTAGCATGTTTTTTTGCTTGGATCGTAGATGGGTGAAAAGAAAAACTGTTCTCTCTTTTCTCTCTCTTTCTGAGGGTTTTGGTTGTTGGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCAACTGCAGCGAAATTGTCTTGGTGCGCATCAAGTCACATGAATGTTTTAGCGATAGTTTGTGGTCATGGAAACTAAACCCTTCATACGCAGACAAGAACGAAATGAAACCAATGTAAGCATCCATCTATCTCTCTTTAGTTATCTCATTTCGAAAATTGATGAAGATAATTGTGGGTCAAAAGAACCATGTCCTTGCCCAAAGCTCTCAAGAAATTTCGAACAACTTTAACTTTCTGTTGCATTCTGTCAAGATAGACAAGAAATGAGCACACACAAACACAAGACAGAGTCACAGAGACACCATGCCATGTCATTTAAAGTAAAGTGTTGTGTTGATGCTTTGTAGAAGTAGATAAAACTAAAATGTTTATATCAAATTCATGCATATTAAGTGTAAATTAGTTTATATGAGCCAGGATGAGATCACTGAAGCAGTTGAACTCACGAAAGCTGTGCGGAGCTCAAGCACGGGACTTGATAAAGAAGCTGCCGCCCAGACTTGTTAGCCACAATTCTTTATCTTTACTTCTTATAGAGCCATGATGTGATGTGATGTGATTTGATATGATGTTCTCATCTTAGGCGCCAAAATGAAATATCAAGCTAGAAAAGGCAGGCCTGTAAAATTTACCTTCTCAATTTTTAGTTGAAAATTGCTGACAAGACTAACTTAATATGTAGGATATATTATGAAATTCTCAATTTTTGTGTGCAACAGGAAAAGTAACTCGATCATAAATGGCATAGTCTGCCTCCTTAGCTTGTAGGTTCAAGATTTGATCCCTATTCCTGTGATTGCCGTACTAAAGTAAATTGTAATTTAACATATGATGTTATACTCTAATATAACAACTTCTTTGGTTTGTAAT

Coding sequence (CDS)

GTCCATAAAAAATGCAGAATCCAACGATTTCCCGCCATGTTAACCGTAGCGCCAAGAACCCTATCTCCTTCACCGTTTCTTCACATCCCACAAACAAACTCCCATTTCCTCACCTCTTCATCCATTCATCTCTTCCAATTTTCACACTCCAACAAAATACCCCTCAACAATTGTCTCTCAAACAATCGCACCGAAAGGGGCTTAGATTTCGACATCGGAGACACCTTCTTCCGTCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTTTACCGGAAATCCAAGGGCCGTCTTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGATCGCTTCGGTACTTGGTCGAGTCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGATGAAAATCGGAGGGTTATTGTGAAGAATCTATCTAGAGTTGCGGAAAATATTGGGGACAATCGGCGGTGGTTGGTTACTAATTGTGATGCGAACAGGGTAATGACGGAGTTTTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGAAGTGATTCGTCGTTTCTGAGACCCGCTATAAATGCTTTGAAATTGGATGGATTGCTCTATGTTACGTCGACGGATGGCTTCTCTTCCGGCGGACATCGGCCTTCTCAATCTTTAGCTGCATACGGAGCATATGTGCGTCCCATGCCATTTTCAAATGAAATTGGTCTGCGGATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTTCTATGCTACTCCACTCTTTTCGTATTATTCGTACCATGGACCTGTTTTCAGAGTCATGCTACGAATCAATCGAGGGAGGATTCATAAAAACAGGCATTATGGTTTTGTTAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGCTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTTGTGGTCTCGGGACCTCTTTGGATTGGACCTCTACACAGTGCAGACTACATCGAAGACATATTAACCTTGGCCAAGCAATGGGAATGGATAGGCAATGGCCAGGGGAAGGATCTTGAGAAACTTCTGAGGCAGATGGTGGATGAGAGCGACTCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGGGCAAAAGTGAATTCACCTCCCCTCAGTACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCAATCAAGACTAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCAACTGCAGCGAAATTGTCTTGGTGCGCATCAAGTCACATGA

Protein sequence

VHKKCRIQRFPAMLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDIGDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIAQLQRNCLGAHQVT

Homology

[Close]

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 1.3e-31
Identity = 105/369 (28.46%), Postives = 180/369 (48.78%), Query Frame = 0


Query: 76  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 135
           F+    A  RD+ V+   L    K R+ VLDAL   GIR +R+ +E+ A+ +  ND +++
Sbjct: 27  FYNPRMALNRDIAVV---LLNVLKPRI-VLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 136 NRRVIVKNL-----SRVAENIG-----DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 195
              ++ KN+       + E+ G       +  +V + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 196 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 255
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 256 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 315
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 316 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 375
                   E+ +   P   +         GP+W+GPL S + +E++L +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 376 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 432
            K   KLL+ + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.9e-31
Identity = 115/373 (30.83%), Postives = 183/373 (49.06%), Query Frame = 0


Query: 76  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 135
           F+    A  RD+ VLA     K+ G  RVLDAL   GIR +RY +E+ A  +  ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 136 NRRVIVKNL-----------SRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVD 195
             R+IV N+            ++A   G+ +  +VTN DANR+M E +    +FDF+D+D
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGE-KTLVVTNADANRLMNEQF---RYFDFVDLD 146

Query: 196 SFGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGL 255
            FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GL
Sbjct: 147 PFGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGL 206

Query: 256 RMLIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGN 315
           R+LIG + R A+        L +YY  H   FR  L++  G    ++    + Y  +   
Sbjct: 207 RILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ--- 266

Query: 316 SQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQG 375
                  E G+      ++ +       GPLW+GPL + +++E++  L+ +   +G+   
Sbjct: 267 ------DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS--- 326

Query: 376 KDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIAS 435
           K  +K L  + DE +  +P  Y     VA R  +    L+ ++  + + GY A+R+H + 
Sbjct: 327 KKTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSP 370

Query: 436 NAIKTNCPMAECI 437
            A+KT+ P  E +
Sbjct: 387 TALKTDAPFEEVL 370

[Close]

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 8.3e-31
Identity = 110/369 (29.81%), Postives = 172/369 (46.61%), Query Frame = 0


Query: 76  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDANDE 135
           F+    A  RD+ VL   L    K ++ VLDAL   GIR +R+ +E+ A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVL---LLNVLKPKI-VLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 136 NRRVIVKNL-----SRVAEN-----IGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDS 195
              ++ KN+       + EN         +  ++ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 196 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 255
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 256 LIGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN---RHYGFVSYCEKCG 315
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 316 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 375
             +                S +S      GPLW+GPL +   + ++L L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 376 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 432
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot
Match: Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.4e-30
Identity = 106/361 (29.36%), Postives = 189/361 (52.35%), Query Frame = 0


Query: 76  FFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVES-EADFVLANDAND 135
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VES +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 136 ENRRVIVKNLSRVAENIG-DNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFL 195
               +I  N    A+N G +N +  + N DAN ++ E        D+ID+D FGS + F+
Sbjct: 95  NATSLIQIN----AKNNGIENAK--IYNKDANALLYEVK-----SDYIDIDPFGSPAPFI 154

Query: 196 RPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVARE 255
             ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + RE
Sbjct: 155 LSSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIRE 214

Query: 256 ASVLGFYATPLFSYYSYHGPVFRVMLRINRGR----IHKNRHYGFVSYCEKCGNSQAFSW 315
           A++L     PLFS+Y+ +   +R+ +R+  G      + N+H  +   C +CG  Q F  
Sbjct: 215 AAILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV- 274

Query: 316 SELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKL 375
            E  +  CP       E   + GPL+IGPL+S ++++ I+ L   + ++ +     ++KL
Sbjct: 275 EENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKL 334

Query: 376 LRQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLSTMMSEMIKAGYAASRSHIASNAIKT 430
           L   V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T
Sbjct: 335 LN--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRT 364

[Close]

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot
Match: Q9YDY7 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=trm1 PE=3 SV=3)

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-28
Identity = 104/374 (27.81%), Postives = 178/374 (47.59%), Query Frame = 0


Query: 74  DTFFRHESATGRDLGVLAAALYRKSKGRLR---VLDALCGCGIRSLRYLVESEADFVLAN 133
           + F+       RD+ V+AA+  R++    R     DA  G G+R +RY VE+    V+ N
Sbjct: 39  EVFYNPAMEFNRDVSVVAASALRRTGLLTRSGVAFDAHAGVGVRGVRYAVEAGYVKVIMN 98

Query: 134 DANDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDF---FDFIDVDSFG 193
           D N +   +   N        G    +++ N ++N +M  F+L ++       ID+D +G
Sbjct: 99  DINPKASMLAALNARANGLEPGS---YMIFNKESNSLM--FHLSRERPTPVSLIDIDPYG 158

Query: 194 SDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYG-AYVRPMPFSNEIGLRML 253
           S + F+  A+       ++ +T+TD     G +   ++  Y    V   P S E GLR+L
Sbjct: 159 SPAPFVDAALALSGKGTVVAMTATDLAVLEGGKARAAVRRYMLRSVSKTPVSKETGLRVL 218

Query: 254 IGGVAREASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKN----RHYGFVSYCEKCG 313
           +G VAR A+       PL +YY+ H   +RV + + RG    +     + G + YC + G
Sbjct: 219 LGYVARVAAAHDKAVKPLLAYYADH--YYRVYVAVERGARRSDSMLEENLGRLVYCPETG 278

Query: 314 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQ 373
            + A S++E    +  C  S V    V + P WIG L    ++E +L +A +  W+G   
Sbjct: 279 VALALSYAE--DPASACGGSYV----VAADPAWIGSLGDQAFLEAMLNIAVEAVWLGT-- 338

Query: 374 GKDLEKLLRQMVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIA 433
              +EKLL  +  E+       Y+ +  VAS+A+VN+P  S ++  +   GY A  +H +
Sbjct: 339 RPRVEKLLNTLHGEAPLSPRSIYVSLTSVASKARVNTPKKSKVVELLRSMGYRAVATHFS 397

Query: 434 SNAIKTNCPMAECI 437
              ++T+ P  E +
Sbjct: 399 GEGVRTDAPWGEVL 397

[Close]

BLAST of Cucsat.G17495 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIAQLQRNCLGAHQVT 452
           AECIRIAQLQRNCLGAHQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]

BLAST of Cucsat.G17495 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >TYK22417.1 tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 849 bits (2194), Expect = 4.66e-310
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIAQLQRNCLGAHQVT 452
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Cucsat.G17495 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 815 bits (2106), Expect = 1.24e-296
Identity = 402/441 (91.16%), Postives = 424/441 (96.15%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           ML+V PRT SPSPFLH PQTNSH L++S+IHLFQFSHSNK  LNNCL NNRTERGL+FDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           G TFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLSRVAE+ GD RRW+VTNCDANRVMT FYL+KDFFDFID+DSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLG YATPLFSYYSYHGPVFRVMLRINRGR++KNRHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVS+SLVVSGPLW GPLHSADYIE++L+LAKQW+WIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIA-QLQRNCLGAHQVT 452
           AECIRIA +LQ+NCLG HQVT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

[Close]

BLAST of Cucsat.G17495 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 799 bits (2063), Expect = 4.43e-290
Identity = 396/441 (89.80%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFY TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIA-QLQRNCLGAHQVT 452
           AECIRIA +LQ++CLG HQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Cucsat.G17495 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 796 bits (2057), Expect = 3.63e-289
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIA-QLQRNCLGAHQVT 452
           AECIRIA +LQ++CLG HQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIAQLQRNCLGAHQVT 452
           AECIRIAQLQRNCLGAHQVT
Sbjct: 421 AECIRIAQLQRNCLGAHQVT 440

[Close]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 849 bits (2194), Expect = 2.26e-310
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIAQLQRNCLGAHQVT 452
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 849 bits (2194), Expect = 2.26e-310
Identity = 420/440 (95.45%), Postives = 428/440 (97.27%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTVA  TLSPSPFL+I QTNSHFLTS SIHLFQFSHSNK   NNCLSNNRTERGLDFDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LR+LDALCGCGIRSLRYLVESEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENRRVIVKNLS+VAEN GDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI KNRHYGFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIAQLQRNCLGAHQVT 452
           A+CIRIAQLQ+NCLG HQVT
Sbjct: 421 ADCIRIAQLQQNCLGVHQVT 440

[Close]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 799 bits (2063), Expect = 2.14e-290
Identity = 396/441 (89.80%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFY TPLFSYYSYHGPVFRVMLRINRGRI+KNRHYGFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIA-QLQRNCLGAHQVT 452
           AECIRIA +LQ++CLG HQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL
Match: A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)

HSP 1 Score: 796 bits (2057), Expect = 1.76e-289
Identity = 395/441 (89.57%), Postives = 415/441 (94.10%), Query Frame = 0


Query: 13  MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 72
           MLTV   TLSPSPFL   QT SH  +SS+IHLFQFS S K PLNNCL NNRTERG++FDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 73  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 132
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 133 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 192
           NDENR VIVKNLSRVAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 193 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 252
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 253 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 312
           EASVLGFY TPLFSYYSYHGPVFRVMLRI+RGRI+KNRHYGFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 313 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 372
           GQMSCPCND+RVSESLVVSGPLW GPLHSADYIED+LTLA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 373 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 432
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 433 AECIRIA-QLQRNCLGAHQVT 452
           AECIRIA +LQ++CLG HQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

The following BLAST results are available for this feature:

BLAST of Cucsat.G17495 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9V1P3	1.3e-31	28.46	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
B6YUU9	2.9e-31	30.83	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
P81554	8.3e-31	29.81	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
Q97ZH0	2.4e-30	29.36	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai...	[more]
Q9YDY7	1.0e-28	27.81	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700...	[more]

BLAST of Cucsat.G17495 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655038.1	0.0	100.00	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR...	[more]
XP_008456934.1	4.66e-310	95.45	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]...	[more]
XP_038892632.1	1.24e-296	91.16	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida]	[more]
XP_022943113.1	4.43e-290	89.80	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]
XP_022986521.1	3.63e-289	89.57	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima]	[more]

BLAST of Cucsat.G17495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP99	0.0	100.00	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A1S3C509	2.26e-310	95.45	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3DFE8	2.26e-310	95.45	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1FWD4	2.14e-290	89.80	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JBD3	1.76e-289	89.57	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 67..277 e-value: 2.7E-38 score: 132.2
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 13..280
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 52..281 score: 61.144688
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 41..281 e-value: 1.2E-58 score: 200.4
None	No IPR available	PANTHER	PTHR10631:SF9	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE	coord: 13..280
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 94..200 e-value: 4.06783E-4 score: 37.0243
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 54..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G17495.T1	Cucsat.G17495.T1	mRNA
Cucsat.G17495.T3	Cucsat.G17495.T3	mRNA
Cucsat.G17495.T4	Cucsat.G17495.T4	mRNA
Cucsat.G17495.T9	Cucsat.G17495.T9	mRNA
Cucsat.G17495.T6	Cucsat.G17495.T6	mRNA
Cucsat.G17495.T2	Cucsat.G17495.T2	mRNA
Cucsat.G17495.T7	Cucsat.G17495.T7	mRNA
Cucsat.G17495.T8	Cucsat.G17495.T8	mRNA
Cucsat.G17495.T5	Cucsat.G17495.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002940	tRNA N2-guanine methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0003723	RNA binding